CN109566269A - A kind of culture medium improving cordycepin content in Cordyceps militaris - Google Patents
A kind of culture medium improving cordycepin content in Cordyceps militaris Download PDFInfo
- Publication number
- CN109566269A CN109566269A CN201811522545.3A CN201811522545A CN109566269A CN 109566269 A CN109566269 A CN 109566269A CN 201811522545 A CN201811522545 A CN 201811522545A CN 109566269 A CN109566269 A CN 109566269A
- Authority
- CN
- China
- Prior art keywords
- parts
- cordyceps militaris
- culture medium
- content
- cordycepin content
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The present invention provides a kind of culture medium for improving cordycepin content in Cordyceps militaris, belongs to Cordyceps militaris cultural technique field.The present invention is a kind of culture medium for improving cordycepin content in Cordyceps militaris, by weight includes following components: 12-16 parts of sucrose, 15-19 parts of potato starch, 15-20 parts of glucose, 13-16 parts of peptone, 0.4-0.7 parts of magnesium sulfate, 0.01-0.012 parts of vitamin B1,1.5-2.1 parts of adenine, 5-18 parts of amino acid 1,4-7 parts of diammonium hydrogen phosphate.Adenine, organic nitrogen source and inorganic nitrogen-sourced combination in culture medium of the invention, can be good at the Proliferation, Differentiation for promoting plant cell, and monosaccharide and polysaccharide composite meet the habit of Cordyceps militaris using can satisfy demand of the Cordyceps militaris to carbohydrate.Amino acids substance is that plant cell is easy to the nitrogen source absorbed, can directly be utilized, it is possible to which the growth of effective stimulus Cordyceps militaris cell and the intake to carbohydrate improve the content of internal polysaccharide, and then improve the content of cordycepin, cordycepic acid.
Description
Technical field
The invention belongs to Cordyceps militaris cultural technique fields, and in particular to a kind of culture for improving cordycepin content in Cordyceps militaris
Base.
Background technique
Cordyceps militaris also known as northern Chinese caterpillar Fungus are to belong to xenogenesis, cordycepin content with China's rare traditional Chinese medicine cordyceps sinensis
It is about 3 to 6 times of cordycepin height in cordyceps sinensis.Cordycepin is one of cordyceps sinensis adenosine class analogue, molecular formula
C10H13O3N5, cordycepin is proved have the effects that antitumor, antiviral, antibacterial, with cytotoxicity and immunoregulation effect.
Cordycepin in Cordyceps militaris is nucleoside antibiotic, and cordycepic acid is a kind of D type mannitol.Improve both substances in Cordyceps militaris
Content can greatly promote Cordyceps militaris to insomnia, rheumatism, rheumatism, the alleviation of cardiovascular disease and adjuvant treatment
Effect.
Currently, the sterling price of cordycepin is 25000 dollars/g in the international market.China's cordyceps militaris plantation is universal, but
Mostly it is used as medicine in the form of mycelium or fructification.
The worldwide distribution natural resources quantity of Cordyceps militaris is seldom.By stroma (i.e. careless part) and sclerotium (the i.e. corpse portion of worm
Point) complex dimerous.Winter larva lives in seclusion in soil, and mushroom parasitism wherein, draws nutrition, is full of mycelia in larva body
And it is dead.To summer, seedling is born from larva corpse, likeness in form grass is acquired before and after the Summer Solstice and obtained.Cordyceps militaris have it is edible and
Medical value.
The artificial cultivation technique of Cordyceps militaris is not perfect at present, and the cordycepin, cordycepic acid content in Cordyceps militaris are relatively low.It is existing
The content of cordycepin is low in the Cordyceps militaris of artificial growth in technology, and effective medical substance that same amount of cordyceps sinensis extracts is relatively
It is few.
Summary of the invention
In view of this, the present invention provides a kind of culture medium for improving cordycepin content in Cordyceps militaris, Cordyceps militaris can be improved
The content of middle cordycepin makes the content of Cordyceps militaris reach 4500-5000mg/kg, and the content of cordycepic acid is in 13-17%.
The present invention is a kind of culture medium for improving cordycepin content in Cordyceps militaris, by weight includes following components: sugarcane
12-16 parts sugared, 15-19 parts of potato starch, 15-20 parts of glucose, 13-16 parts of peptone, 0.4-0.7 parts of magnesium sulfate, vitamin
0.01-0.012 parts of B1,1.5-2.1 parts of adenine, 5-18 parts of amino acid 1,4-7 parts of diammonium hydrogen phosphate.
A kind of more preferred scheme is, by weight comprising following components: 15 parts of sucrose, 16 parts of potato starch, Portugal
Grape sugar 16 parts, 15 parts of peptone, 0.5 part of magnesium sulfate, 0.011 part of vitamin B1,2 parts of adenine, 6 parts of amino acid 1, phosphoric acid hydrogen
6 parts of diammonium.
Wherein, amino acid can be one of glycine, serine, glutamine, asparagine or a variety of.More preferably
Amino acid be glycine.Peptone, can greatly be beneficial for Cordyceps militaris growth by potato starch as main nitrogen source,
Sucrose and glucose provide nutrition as carbon source main in culture medium for tissue growth, and Cordyceps militaris is trained during culture
Feeding body can directly absorb the glucose as monosaccharide, while sucrose, for feeding nutrient in due course, Cordyceps militaris mushroom hobby sugar is sufficient
Sugar can promote its growth and breeding.Adenine is added as the basic element of cell division simultaneously, promotes the formation of bud, at this moment cell division
Element plays leading role, promotes the Proliferation, Differentiation of cell.In addition, amino acid, as important organic nitrogen source, diammonium hydrogen phosphate is
Inorganic nitrogen-sourced, organic nitrogen source, inorganic nitrogen-sourced comprehensive function can promote the growth of culture to greatest extent, make jointly with carbohydrate
Promote Cordyceps militaris fungus grown, the effect of differentiation well with can be realized, is mentioned conducive to cordycepin content in Cordyceps militaris mushroom
It is high.
Preferably, by weight further including 0.8-1.2 parts of potassium dihydrogen phosphate, 0.005-0.01 parts of auxin, life
Long element can be one or both of heteroauxin, methyl α-naphthyl acetate.For potassium dihydrogen phosphate as buffer, Cordyceps militaris is that slant acidity is true
Bacterium, mycelia growth and development optimal pH are 5.2-6.8, and buffer substance, which is added, can simulate the natural life cycle of Cordyceps militaris mushroom
Border provides a good growing environment for it, but the additive amount of buffer substance can not be excessive or very few, otherwise can make pH
Value goes beyond the scope, and inhibits the breeding of plant cell；In tissue culture, the effect of auxins is the formation of evoked callus, embryo shape
The generation of body, suitable auxin can promote the differentiation of incubation plant cell well.
It can also add, inositol 0.08-0.12 parts by weight, 0.006-0.01 parts of antibiotic.Inositol is called hexamethylene
Six alcohol play an important role in the mutually converting of carbohydrate, and suitably use inositol, can promote growth and the embryoid of callus
With the formation of bud.There is facilitation to breeding, the differentiation of tissue and cell, also has effect to the formation of cell wall.Antibiotic
Addition can prevent Browning, reduce the loss of material in culture.
The specific cultural method of Cordyceps militaris based on culture medium of the invention is as follows: use bacterial strain deposit number for
The Cordyceps militaris spawn of CGMCC3.4655 is inoculated on PDA plate culture medium and is activated, then the bacterial strain after activation is connected to kind
In sub- culture medium, seed liquor is obtained within liquid deep layer fermenting culture 3 days.Then seed liquor is inoculated into fermentation medium, liquid
Cordycepin precursor mixing additive adenine and glycine are added after Submerged fermentation 5 days, is cultivated for terminating for 5 days.It adopts
Submerged fermentation is carried out with the fluid nutrient medium.
Culture medium addition adenine, organic nitrogen source and inorganic nitrogen-sourced combination of the invention can be good at promoting plant cell
Proliferation, Differentiation, monosaccharide and polysaccharide composite meet the habit of Cordyceps militaris using can satisfy demand of the Cordyceps militaris to carbohydrate.
Amino acids substance is that plant cell is easy to the nitrogen source absorbed, can directly be utilized, it is possible to which effective stimulus Cordyceps militaris is thin
The growth of born of the same parents and intake to carbohydrate improve the content of internal polysaccharide, and then improve the content and cordycepic acid of cordycepin, cordycepic acid
Content.
Specific embodiment
The present invention will be described in detail With reference to embodiment.
Embodiment one
The present embodiment is a kind of culture medium for improving cordycepin content in Cordyceps militaris, by weight includes following components: sucrose
15 parts, 16 parts of potato starch, 16 parts of glucose, 15 parts of peptone, 0.5 part of magnesium sulfate, 0.011 part of vitamin B1, adenine 2
Part, 16 parts of glycine, 6 parts of diammonium hydrogen phosphate, 1 part of potassium dihydrogen phosphate, 0.008 part of heteroauxin, 0.10 part of inositol, antibiotic
0.008 part.
The specific cultural method of the Cordyceps militaris of culture medium based on the present embodiment is as follows: use bacterial strain deposit number for
The Cordyceps militaris spawn of CGMCC3.4655 is inoculated on PDA plate culture medium and is activated, then the bacterial strain after activation is connected to kind
In sub- culture medium, seed liquor is obtained within liquid deep layer fermenting culture 3 days.Then seed liquor is inoculated into fermentation medium, liquid
Cordycepin precursor mixing additive adenine and glycine are added after Submerged fermentation 5 days, is cultivated for terminating for 5 days.It adopts
Submerged fermentation is carried out with the fluid nutrient medium.The biomass of Cordyceps militaris mycelia is observed after the completion of culture, measures cordycepin
The content of yield and cordycepic acid.
Embodiment two
The present embodiment is a kind of culture medium for improving cordycepin content in Cordyceps militaris, by weight includes following components: sucrose
12 parts, 15 parts of potato starch, 15 parts of glucose, 13 parts of peptone, 0.4 part of magnesium sulfate, 0.01 part of vitamin B1, adenine 1.5
Part, 15 parts of serine, 4 parts of diammonium hydrogen phosphate, 0.8 part of potassium dihydrogen phosphate, 0.005 part of heteroauxin, 0.08 part of inositol, antibiotic
0.006 part.
The specific cultural method of the Cordyceps militaris of culture medium based on the present embodiment is as follows: use bacterial strain deposit number for
The Cordyceps militaris spawn of CGMCC3.4655 is inoculated on PDA plate culture medium and is activated, then the bacterial strain after activation is connected to kind
In sub- culture medium, seed liquor is obtained within liquid deep layer fermenting culture 3 days.Then seed liquor is inoculated into fermentation medium, liquid
Cordycepin precursor mixing additive adenine and glycine are added after Submerged fermentation 5 days, is cultivated for terminating for 5 days.It adopts
Submerged fermentation is carried out with the fluid nutrient medium.The biomass of Cordyceps militaris mycelia is observed after the completion of culture, measures cordycepin
The content of yield and cordycepic acid.
Embodiment three
The present embodiment is a kind of culture medium for improving cordycepin content in Cordyceps militaris, by weight includes following components: sucrose
16 parts, 19 parts of potato starch, 20 parts of glucose, 16 parts of peptone, 0.7 part of magnesium sulfate, 0.012 part of vitamin B1, adenine
2.1 parts, 18 parts of glutamine, 7 parts of diammonium hydrogen phosphate, 1.2 parts of potassium dihydrogen phosphate, 0.01 part of methyl α-naphthyl acetate, 0.12 part of inositol, antibiosis
0.01 part of element.
The specific cultural method of the Cordyceps militaris of culture medium based on the present embodiment is as follows: use bacterial strain deposit number for
The Cordyceps militaris spawn of CGMCC3.4655 is inoculated on PDA plate culture medium and is activated, then the bacterial strain after activation is connected to kind
In sub- culture medium, seed liquor is obtained within liquid deep layer fermenting culture 3 days.Then seed liquor is inoculated into fermentation medium, liquid
Cordycepin precursor mixing additive adenine and glycine are added after Submerged fermentation 5 days, is cultivated for terminating for 5 days.It adopts
Submerged fermentation is carried out with the fluid nutrient medium.The biomass of Cordyceps militaris mycelia is observed after the completion of culture, measures cordycepin
The content of yield and cordycepic acid.
Example IV
The present embodiment is a kind of culture medium for improving cordycepin content in Cordyceps militaris, by weight includes following components: sucrose
15 parts, 16 parts of potato starch, 19 parts of glucose, 15 parts of peptone, 0.7 part of magnesium sulfate, 0.012 part of vitamin B1, adenine
2.1 parts, 18 parts of asparagine, 7 parts of diammonium hydrogen phosphate, 1.1 parts of potassium dihydrogen phosphate, 0.12 part of inositol, 0.007 part of antibiotic.
The specific cultural method of the Cordyceps militaris of culture medium based on the present embodiment is as follows: use bacterial strain deposit number for
The Cordyceps militaris spawn of CGMCC3.4655 is inoculated on PDA plate culture medium and is activated, then the bacterial strain after activation is connected to kind
In sub- culture medium, seed liquor is obtained within liquid deep layer fermenting culture 3 days.Then seed liquor is inoculated into fermentation medium, liquid
Cordycepin precursor mixing additive adenine and glycine are added after Submerged fermentation 5 days, is cultivated for terminating for 5 days.It adopts
Submerged fermentation is carried out with the fluid nutrient medium.The biomass of Cordyceps militaris mycelia is observed after the completion of culture, measures cordycepin
The content of yield and cordycepic acid.
The content of the cordycepin for the Cordyceps militaris that embodiment one is cultivated into example IV is 4500-5000mg/kg, cordycepic acid
Content 13-17%.
Culture medium addition adenine, organic nitrogen source and inorganic nitrogen-sourced combination of the invention can be good at promoting plant cell
Proliferation, Differentiation, monosaccharide and polysaccharide composite meet the habit of Cordyceps militaris using can satisfy demand of the Cordyceps militaris to carbohydrate.
Amino acids substance is that plant cell is easy to the nitrogen source absorbed, can directly be utilized, it is possible to which effective stimulus Cordyceps militaris is thin
The growth of born of the same parents and intake to carbohydrate improve the content of internal polysaccharide, and then improve the content of cordycepin, cordycepic acid.Add simultaneously
Some substances for promoting plant cell growth are added to improve the differentiation speed of cell, the method for the present invention technological operation is simple, and the period is short,
It is suitble to large-scale production.
The present invention is not limited to above-mentioned specific embodiment, and the invention may be variously modified and varied.All foundations
Technical spirit of the invention should be included in the present invention to embodiment of above any modification, equivalent replacement, improvement and so on
Protection scope.
Claims (8)
1. a kind of culture medium for improving cordycepin content in Cordyceps militaris, which is characterized in that by weight include following components: sugarcane
12-16 parts sugared, 15-19 parts of potato starch, 15-20 parts of glucose, 13-16 parts of peptone, 0.4-0.7 parts of magnesium sulfate, vitamin
0.01-0.012 parts of B1,1.5-2.1 parts of adenine, 5-18 parts of amino acid 1,4-7 parts of diammonium hydrogen phosphate.
2. a kind of culture medium for improving cordycepin content in Cordyceps militaris according to claim 1, which is characterized in that by weight
Part meter includes following components: 15 parts of sucrose, 16 parts of potato starch, 16 parts of glucose, 15 parts of peptone, 0.5 part of magnesium sulfate, dimension life
0.011 part of plain B1,2 parts of adenine, 6 parts of amino acid 1,6 parts of diammonium hydrogen phosphate.
3. a kind of culture medium for improving cordycepin content in Cordyceps militaris according to claim 1, which is characterized in that by weight
Part meter further includes 0.8-1.2 parts of potassium dihydrogen phosphate.
4. a kind of culture medium for improving cordycepin content in Cordyceps militaris according to claim 1, which is characterized in that by weight
Part meter further includes 0.005-0.01 parts of auxin.
5. a kind of culture medium for improving cordycepin content in Cordyceps militaris according to claim 4, which is characterized in that the life
Long element is one or both of heteroauxin, methyl α-naphthyl acetate.
6. a kind of culture medium for improving cordycepin content in Cordyceps militaris according to claim 1, which is characterized in that by weight
Part meter further includes 0.08-0.12 parts of inositol, 0.006-0.01 parts of antibiotic.
7. a kind of culture medium for improving cordycepin content in Cordyceps militaris according to claim 1, which is characterized in that the ammonia
Base acid is one of glycine, serine, glutamine, asparagine or a variety of.
8. a kind of culture medium for improving cordycepin content in Cordyceps militaris according to claim 1, which is characterized in that the ammonia
Base acid is glycine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811522545.3A CN109566269A (en) | 2018-12-13 | 2018-12-13 | A kind of culture medium improving cordycepin content in Cordyceps militaris |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811522545.3A CN109566269A (en) | 2018-12-13 | 2018-12-13 | A kind of culture medium improving cordycepin content in Cordyceps militaris |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109566269A true CN109566269A (en) | 2019-04-05 |
Family
ID=65928358
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811522545.3A Pending CN109566269A (en) | 2018-12-13 | 2018-12-13 | A kind of culture medium improving cordycepin content in Cordyceps militaris |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109566269A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110447463A (en) * | 2019-08-12 | 2019-11-15 | 江门市山海堂虫草有限公司 | A kind of preparation method of cordyceps sporophore |
| CN110637677A (en) * | 2019-08-12 | 2020-01-03 | 江门市山海堂虫草有限公司 | Culture medium for improving cordycepin of cordyceps militaris and preparation method thereof |
| CN112586513A (en) * | 2020-12-11 | 2021-04-02 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
| CN113980820A (en) * | 2021-11-04 | 2022-01-28 | 锬酃藏虫草生物科技(深圳)有限公司 | Method for culturing cordycepin and adenosine contents in cordyceps militaris |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105410034A (en) * | 2015-11-27 | 2016-03-23 | 湖北工业大学 | Compound promoter for cordyceps sinensis cultivation and preparation method thereof |
| CN105875182A (en) * | 2014-11-07 | 2016-08-24 | 江苏学府生物工程有限公司 | Method for producing Cordyceps militaris with high cordycepin content |
| CN106550764A (en) * | 2015-09-30 | 2017-04-05 | 黄友鹰 | A kind of SOYBEAN IN HIGH-YIELD BREEDING method of Cordyceps sinensis |
-
2018
- 2018-12-13 CN CN201811522545.3A patent/CN109566269A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105875182A (en) * | 2014-11-07 | 2016-08-24 | 江苏学府生物工程有限公司 | Method for producing Cordyceps militaris with high cordycepin content |
| CN106550764A (en) * | 2015-09-30 | 2017-04-05 | 黄友鹰 | A kind of SOYBEAN IN HIGH-YIELD BREEDING method of Cordyceps sinensis |
| CN105410034A (en) * | 2015-11-27 | 2016-03-23 | 湖北工业大学 | Compound promoter for cordyceps sinensis cultivation and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 张峰，陈丽静主编: "《细胞工程》", 31 July 2014, 中国农业大学出版社 * |
| 郑鑫，陶春雨，徐嘉潞，等: "3种生长素对蛹虫草液体发酵的影响", 《中国食用菌》 * |
| 陈若芸主编；康洁副主编: "《中国食用药用真菌化学》", 31 March 2016, 上海科学技术文献出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110447463A (en) * | 2019-08-12 | 2019-11-15 | 江门市山海堂虫草有限公司 | A kind of preparation method of cordyceps sporophore |
| CN110637677A (en) * | 2019-08-12 | 2020-01-03 | 江门市山海堂虫草有限公司 | Culture medium for improving cordycepin of cordyceps militaris and preparation method thereof |
| CN112586513A (en) * | 2020-12-11 | 2021-04-02 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
| CN112586513B (en) * | 2020-12-11 | 2021-11-19 | 辽宁省海洋水产科学研究院 | Pure natural cordyceps militaris growth regulator |
| CN113980820A (en) * | 2021-11-04 | 2022-01-28 | 锬酃藏虫草生物科技(深圳)有限公司 | Method for culturing cordycepin and adenosine contents in cordyceps militaris |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102405763B (en) | Cultivating method of aweto | |
| CN103125275B (en) | Cultivation method of cordyceps militaris | |
| CN109566269A (en) | A kind of culture medium improving cordycepin content in Cordyceps militaris | |
| CN108484314A (en) | It is a kind of promote hickory chick growth microorganism liquid seaweed extract and its application | |
| CN103299826A (en) | Cordyceps militaris method capable of improving content of cordycepin | |
| CN108450230B (en) | Oyster mushroom cultivation method | |
| CN101565689A (en) | Production method for high-density pure arbuscular mycorrhizal fungal spore | |
| CN100575475C (en) | The method of wheat cultivation Cordyceps militaris | |
| CN105085050A (en) | Nutrient medium for okra soilless culture and preparation method thereof | |
| CN109566299A (en) | A kind of high yield spinach cultural method | |
| CN1986827B (en) | Truffle polyose preparing process | |
| CN104072299B (en) | A kind of needle mushroom liquid spawn culture medium and preparation method thereof | |
| CN103250638A (en) | Chrysanthemum tissue culture method | |
| CN100438750C (en) | Application of endogenous fungus in cultivation of snow lotus seedling and snow lotus | |
| CN103005437B (en) | Method for preparing Se-enriched bran edible mushroom nutrition powder by solid cultivation | |
| CN109699394A (en) | A method of vertical stripe Xylaria sp. fungus fructification is produced using fluid nutrient medium | |
| CN100564510C (en) | A kind of substratum of liquid deep fermentation for producing truffle polysaccharide | |
| CN113330984A (en) | Artificial cultivation method for improving cordyceps sobolifera polysaccharide content in cordyceps sobolifera sporocarp | |
| TWI385248B (en) | A formula of culturing medium for cordyceps spp. | |
| CN101849478B (en) | Coprinus atramentarius and cultivation method thereof | |
| CN103981103A (en) | DSE (Dark Septate Endophyte) strain J-N3 and applications thereof in dendrobium candidum production | |
| CN107099465B (en) | Growth-promoting bacterial strain and its microorganism seedling medium for promoting cuttage tea shoot to take root | |
| CN105638368B (en) | A kind of oil introduction method of tree peony | |
| CN101407767A (en) | Method for producing Chinese caterpillar fungus by fermentation | |
| KR101063754B1 (en) | Cultivation method for Mycoleptodonoides aitchisonii |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190405 |