CN110637677A - Culture medium for improving cordycepin of cordyceps militaris and preparation method thereof - Google Patents
Culture medium for improving cordycepin of cordyceps militaris and preparation method thereof Download PDFInfo
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- CN110637677A CN110637677A CN201910737522.2A CN201910737522A CN110637677A CN 110637677 A CN110637677 A CN 110637677A CN 201910737522 A CN201910737522 A CN 201910737522A CN 110637677 A CN110637677 A CN 110637677A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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Abstract
The invention discloses a cordyceps militaris culture medium for increasing cordycepin content, which comprises a liquid fermentation liquid culture medium and a fruiting body solid culture medium, and is characterized in that: the liquid fermentation broth culture medium comprises the following components in percentage by mass: 15-20% of potato, 1.5-2% of white granulated sugar, 1-2% of egg white, 1-2% of beef extract, 0.1-0.2% of adenine, 0.2-0.4% of glycine and 70-80% of distilled water; the solid culture medium for the sporocarp comprises the following components: 20 to 30 percent of rice, 5 to 10 percent of crushed corn kernels, 2 to 3 percent of white granulated sugar, 1 to 2 percent of silkworm chrysalis meal, 0.5 to 1 percent of adenine, 1 to 2 percent of glutamine and 55 to 65 percent of distilled water. The invention also provides a preparation method of the cordyceps militaris culture medium for improving the cordycepin content, and the preparation method of the combination of the liquid fermentation medium and the solid fruiting body culture medium can effectively stimulate the growth of cordyceps militaris cells and absorb sugars to improve the cordycepin content.
Description
Technical Field
The invention relates to the technical field of cordyceps militaris cultivation, in particular to a culture medium for improving cordyceps militaris cordycepin, and a preparation method thereof.
Background
Cordycepin, also known as Cordyceps sinensis extract, cordycepin, and cordycepin, is the main active ingredient of Cordyceps sinensis and Cordyceps militaris; cordycepin has the effects of bacteriostasis, tumor resistance, inflammation resistance, metabolism improvement, free radical removal and the like, and at the present stage, cordycepin is becoming an extremely active field in medicinal chemistry, anti-aging, beauty treatment and health care products.
The quantity of the natural resources of the cordyceps militaris distributed worldwide is very small. A complex consisting of a stroma (i.e., the grass portion) and a sclerotium (i.e., the cadaver portion of the insect). In winter, the larva hibernates in soil, fungi are parasitized in the soil, the larva absorbs nutrition, and the larva is full of hyphae to die. In summer, seedlings grow from the bodies of the young insect corpses, are similar to grass, and are collected before and after summer. The Cordyceps militaris has edible and medicinal values.
However, in the prior art, the cordycepin content in wild cordyceps sinensis is very low, and is generally extracted from cordyceps militaris, and because the existing cordyceps militaris culture medium lacks substances for promoting cordycepin synthesis, the cordycepin content in cordyceps militaris is low, the utilization value of cordyceps militaris is directly reduced, and the input cost is increased.
Disclosure of Invention
On one hand, the technical problem to be solved by the invention is to provide the cordyceps militaris culture medium for improving the cordycepin content of cordyceps militaris, and the culture medium effectively improves the cordycepin content of cordyceps militaris.
In order to solve the technical problems, the technical scheme of the invention is as follows:
a Cordyceps militaris culture medium for increasing cordycepin content comprises a liquid fermentation medium and a fruiting body solid medium: the liquid fermentation broth culture medium comprises the following components in percentage by mass: 15-20% of potato, 1.5-2% of white granulated sugar, 1-2% of egg white, 1-2% of beef extract, 0.1-0.2% of adenine, 0.2-0.4% of glycine and 70-80% of distilled water; the solid culture medium for the sporocarp comprises the following components: 20 to 30 percent of rice, 5 to 10 percent of crushed corn kernels, 2 to 3 percent of white granulated sugar, 1 to 2 percent of silkworm chrysalis meal, 0.5 to 1 percent of adenine, 1 to 2 percent of glutamine and 55 to 65 percent of distilled water.
Preferably, the liquid fermentation broth culture medium comprises the following components in percentage by mass: 15-17% of potato, 1.5-1.8% of white granulated sugar, 1-1.5% of egg white, 1-1.5% of beef extract, 0.1-0.15% of adenine, 0.2-0.3% of glycine and 70-75% of distilled water; the solid culture medium for the sporocarp comprises the following components: 20 to 25 percent of rice, 5 to 8 percent of crushed corn kernels, 2 to 2.5 percent of white granulated sugar, 1 to 1.5 percent of silkworm chrysalis meal, 0.5 to 0.75 percent of adenine, 1 to 1.5 percent of glutamine and 55 to 60 percent of distilled water.
Adenine acts as a cytokinin to promote the formation of buds, and at this time, the cytokinin plays a leading role to promote the proliferation and differentiation of cells.
Glycine and glutamine are nitrogen sources that plant cells can easily absorb and can be directly utilized.
More preferably, the liquid fermentation broth culture medium comprises the following components in percentage by mass: 17-20% of potato, 1.8-2% of white granulated sugar, 1.5-2% of egg white, 1.5-2% of beef extract, 0.15-0.2% of adenine, 0.3-0.4% of glycine and 75-80% of distilled water; the solid culture medium for the sporocarp comprises the following components: 25 to 30 percent of rice, 8 to 10 percent of crushed corn kernels, 2.5 to 3 percent of white granulated sugar, 1.5 to 2 percent of silkworm chrysalis meal, 0.75 to 1 percent of adenine, 1.5 to 2 percent of glutamine and 60 to 65 percent of distilled water.
Particularly preferably, the liquid fermentation broth culture medium comprises the following components in percentage by mass: 17% of potatoes, 1.8% of white granulated sugar, 1.5% of egg white, 1.5% of beef extract, 0.15% of adenine, 0.3% of glycine and 75% of distilled water; the solid culture medium for the sporocarp comprises the following components: 25% of rice, 8% of crushed corn kernels, 2.5% of white granulated sugar, 1.5% of silkworm chrysalis meal, 0.75% of adenine, 1.5% of glutamine and 60% of distilled water.
The invention also provides a preparation method of the cordyceps militaris culture medium for improving the cordycepin content, which comprises the following steps: the method comprises the following steps:
s1) raw material preparation: preparing raw materials of a liquid fermentation liquid culture medium according to mass percentage;
s2) dissolving and subpackaging the potatoes: peeling potato, squeezing, mixing with the above materials, dissolving, packaging into 500ml triangular flask, sterilizing at 121 deg.C under high pressure, and cooling;
s3) inoculating strains: inoculating slant strain into cooled liquid fermentation culture medium, and culturing at 22-25 deg.C for 7-10 days;
s4) preparing raw materials of the fruiting body solid culture medium: preparing raw materials of a sporophore solid culture medium according to mass percentage;
s5) white sugar, adenine and glutamine miscibility: mixing white granulated sugar, adenine, glutamine and distilled water for dissolving;
s6) mixing and stirring starch: and (3) uniformly mixing and stirring rice, crushed corn kernels and silkworm chrysalis meal, adding the mixture into the step S5), continuously stirring and mixing, carrying out high-pressure sterilization at 121 ℃, and cooling to obtain the culture medium.
Preferably, the culture temperature and the culture time are 25 ℃ and 10 days, respectively.
By adopting the technical scheme, the culture medium is added with adenine, so that the proliferation and differentiation of plant cells can be well promoted, the compound use of monosaccharide and polysaccharide can meet the requirement of cordyceps militaris on saccharides, the growth habit of cordyceps militaris can be met, the growth of cordyceps militaris cells and the uptake of saccharides can be effectively stimulated, the content of polysaccharides in vivo is increased, and the content of cordycepin and cordycepic acid and the content of cordycepic acid are increased. Furthermore, adenine acts as a cytokinin to promote the proliferation and differentiation of cells. In addition, glycine and glutamine are nitrogen sources which are easily absorbed by plant cells and can be directly utilized, so that the growth of cordyceps militaris cells and the intake of saccharides can be effectively stimulated, the content of polysaccharide in vivo is increased, and the content of cordycepin is further increased.
Detailed Description
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Example 1
The invention provides a cordyceps militaris culture medium for increasing cordycepin content, which comprises a liquid fermentation liquid culture medium and a fruiting body solid culture medium: the liquid fermentation broth culture medium comprises the following components in percentage by mass: 15% of potatoes, 1.5% of white granulated sugar, 1% of egg white, 1% of beef extract, 0.1% of adenine, 0.2% of glycine and 70% of distilled water; the solid culture medium for the sporocarp comprises the following components: 20% of rice, 5% of crushed corn kernels, 2% of white granulated sugar, 1% of silkworm chrysalis meal, 0.5% of adenine, 1% of glutamine and 55% of distilled water.
The invention also provides a preparation method of the cordyceps militaris culture medium, which comprises the following steps:
s1) raw material preparation: preparing raw materials of a liquid fermentation liquid culture medium according to mass percentage;
s2) dissolving and subpackaging the potatoes: peeling potato, squeezing, mixing with the above materials, dissolving, packaging into 500ml triangular flask, sterilizing at 121 deg.C under high pressure, and cooling;
s3) inoculating strains: inoculating slant strains into the cooled liquid fermentation medium, and culturing at 25 deg.C for 10 days;
s4) preparing raw materials of the fruiting body solid culture medium: preparing raw materials of a sporophore solid culture medium according to mass percentage;
s5) white sugar, adenine and glutamine miscibility: mixing white granulated sugar, adenine, glutamine and distilled water for dissolving;
s6) mixing and stirring starch: and (3) uniformly mixing and stirring rice, crushed corn kernels and silkworm chrysalis meal, adding the mixture into the step S5), continuously stirring and mixing, carrying out high-pressure sterilization at 121 ℃, and cooling to obtain the culture medium.
Example 2
A Cordyceps militaris culture medium for increasing cordycepin content comprises a liquid fermentation medium and a fruiting body solid medium: the liquid fermentation broth culture medium comprises the following components in percentage by mass: 17% of potatoes, 1.8% of white granulated sugar, 1.5% of egg white, 1.5% of beef extract, 0.15% of adenine, 0.3% of glycine and 75% of distilled water; the solid culture medium for the sporocarp comprises the following components: 25% of rice, 8% of crushed corn kernels, 2.5% of white granulated sugar, 1.5% of silkworm chrysalis meal, 0.75% of adenine, 1.5% of glutamine and 60% of distilled water.
The cordyceps militaris culture medium of example 2 is prepared according to the preparation method of example 1.
Example 3
A Cordyceps militaris culture medium for increasing cordycepin content comprises a liquid fermentation medium and a fruiting body solid medium: the liquid fermentation broth culture medium comprises the following components in percentage by mass: 20% of potato, 2% of white granulated sugar, 2% of egg white, 2% of beef extract, 0.2% of adenine, 0.4% of glycine and 80% of distilled water; the solid culture medium for the sporocarp comprises the following components: 30% of rice, 10% of crushed corn kernels, 3% of white granulated sugar, 2% of silkworm chrysalis meal, 1% of adenine, 2% of glutamine and 65% of distilled water.
The cordyceps militaris culture medium described in example 3 was prepared according to the preparation method of example 1.
The cordycepin content of examples 1-3 was increased as described in Table one
The embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.
Claims (6)
1. A cordyceps militaris culture medium for increasing cordycepin content of cordyceps militaris comprises a liquid fermentation liquid culture medium and a fruiting body solid culture medium, and is characterized in that: the liquid fermentation broth culture medium comprises the following components in percentage by mass: 15-20% of potato, 1.5-2% of white granulated sugar, 1-2% of egg white, 1-2% of beef extract, 0.1-0.2% of adenine, 0.2-0.4% of glycine and 70-80% of distilled water; the solid culture medium for the sporocarp comprises the following components: 20 to 30 percent of rice, 5 to 10 percent of crushed corn kernels, 2 to 3 percent of white granulated sugar, 1 to 2 percent of silkworm chrysalis meal, 0.5 to 1 percent of adenine, 1 to 2 percent of glutamine and 55 to 65 percent of distilled water.
2. The cordyceps militaris culture medium according to claim 1, wherein: the liquid fermentation broth culture medium comprises the following components in percentage by mass: 15-17% of potato, 1.5-1.8% of white granulated sugar, 1-1.5% of egg white, 1-1.5% of beef extract, 0.1-0.15% of adenine, 0.2-0.3% of glycine and 70-75% of distilled water; the solid culture medium for the sporocarp comprises the following components: 20 to 25 percent of rice, 5 to 8 percent of crushed corn kernels, 2 to 2.5 percent of white granulated sugar, 1 to 1.5 percent of silkworm chrysalis meal, 0.5 to 0.75 percent of adenine, 1 to 1.5 percent of glutamine and 55 to 60 percent of distilled water.
3. The cordyceps militaris culture medium according to claim 1, wherein: the liquid fermentation broth culture medium comprises the following components in percentage by mass: 17-20% of potato, 1.8-2% of white granulated sugar, 1.5-2% of egg white, 1.5-2% of beef extract, 0.15-0.2% of adenine, 0.3-0.4% of glycine and 75-80% of distilled water; the solid culture medium for the sporocarp comprises the following components: 25 to 30 percent of rice, 8 to 10 percent of crushed corn kernels, 2.5 to 3 percent of white granulated sugar, 1.5 to 2 percent of silkworm chrysalis meal, 0.75 to 1 percent of adenine, 1.5 to 2 percent of glutamine and 60 to 65 percent of distilled water.
4. The cordyceps militaris culture medium according to claim 1, wherein: the liquid fermentation broth culture medium comprises the following components in percentage by mass: 17% of potatoes, 1.8% of white granulated sugar, 1.5% of egg white, 1.5% of beef extract, 0.15% of adenine, 0.3% of glycine and 75% of distilled water; the solid culture medium for the sporocarp comprises the following components: 25% of rice, 8% of crushed corn kernels, 2.5% of white granulated sugar, 1.5% of silkworm chrysalis meal, 0.75% of adenine, 1.5% of glutamine and 60% of distilled water.
5. A method for preparing the Cordyceps militaris culture medium as claimed in any one of claims 1 to 4, comprising the steps of:
s1) raw material preparation: preparing raw materials of a liquid fermentation liquid culture medium according to mass percentage;
s2) dissolving and subpackaging the potatoes: peeling potato, squeezing, mixing with the above materials, dissolving, packaging into 500ml triangular flask, sterilizing at 121 deg.C under high pressure, and cooling;
s3) inoculating strains: inoculating slant strain into cooled liquid fermentation culture medium, and culturing at 22-25 deg.C for 7-10 days;
s4) preparing raw materials of the fruiting body solid culture medium: preparing raw materials of a sporophore solid culture medium according to mass percentage;
s5) white sugar, adenine and glutamine miscibility: mixing white granulated sugar, adenine, glutamine and distilled water for dissolving;
s6) mixing and stirring starch: and (3) uniformly mixing and stirring rice, crushed corn kernels and silkworm chrysalis meal, adding the mixture into the step S5), continuously stirring and mixing, carrying out high-pressure sterilization at 121 ℃, and cooling to obtain the culture medium.
6. The preparation method of the cordyceps militaris culture medium according to claim 5, wherein the preparation method comprises the following steps: step S3), the strain is inoculated, and the culture temperature and the culture time are respectively 25 ℃ and 10 days.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111826410A (en) * | 2020-08-10 | 2020-10-27 | 辽东学院 | Method for obtaining high-yield cordycepin by using solid culture medium |
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| US20160345504A1 (en) * | 2015-05-29 | 2016-12-01 | Tunghai University | Method for culturing ophiocordyceps sinensis |
| JP2017042125A (en) * | 2015-08-28 | 2017-03-02 | 株式会社ウエルネス四万十研究所 | Cordyceps militaris having various efficacy, and production method thereof |
| CN109566269A (en) * | 2018-12-13 | 2019-04-05 | 湖州柳荫生物科技有限公司 | A kind of culture medium improving cordycepin content in Cordyceps militaris |
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- 2019-08-12 CN CN201910737522.2A patent/CN110637677A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1445360A (en) * | 2002-03-20 | 2003-10-01 | 中国科学院沈阳应用生态研究所 | Method of artificial planting northerly Chinese caterpillar fungus for increasing content of physiologically active substance |
| CN101707966A (en) * | 2009-04-14 | 2010-05-19 | 徐正华 | Living Chinese caterpillar fungi artificially cultivated by using larvae as hosts and method for cultivating same |
| US20160345504A1 (en) * | 2015-05-29 | 2016-12-01 | Tunghai University | Method for culturing ophiocordyceps sinensis |
| JP2017042125A (en) * | 2015-08-28 | 2017-03-02 | 株式会社ウエルネス四万十研究所 | Cordyceps militaris having various efficacy, and production method thereof |
| CN105505784A (en) * | 2015-12-11 | 2016-04-20 | 辽宁省农业科学院食用菌研究所 | Cordyceps militaris culture medium capable of improving cordycepin content, and preparation method of cordyceps militaris culture medium |
| CN109566269A (en) * | 2018-12-13 | 2019-04-05 | 湖州柳荫生物科技有限公司 | A kind of culture medium improving cordycepin content in Cordyceps militaris |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111826410A (en) * | 2020-08-10 | 2020-10-27 | 辽东学院 | Method for obtaining high-yield cordycepin by using solid culture medium |
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