200944120 九、發明說明： 【發明所屬之技術領域】 本創作係相關於牛樟芝的人工栽培方法，特β 種不需以保育類牛樟樹作為栽培介質的牛 、别是指一 法。 彈之人工栽培方 【先前技術】 材：係為珍貴的真菌類藥 材，其生長在台灣保育類樹種牛樟樹上。牛樟* 藥 眾多，其中最被重視的議題在於，三帖類化合研究 、帛重要的化學成分之-，三帖類化合物使牛樟芝：：樟芝 的苦味，牛樟芝的三帖類化合物含量遠超過靈《。、目=特 知三帖類化合物的功能在於，具有防癌、保肝、降 功效。另外，牛樟芝中所含其他化合物，例如多骑體 氧歧化酵素、'㈣、η㈣时生理活性成份，均足以。 對人體生理機能發揮均衡保健的功效。 ⑩ 时的牛樟芝人卫栽培方法，係有使用椴木栽培法、 固體栽培法及液體發酵法等。椴木栽培法係使用枯死 樟樹椴木’將牛樟芝菌體植入椴木中，使牛樟芝在椴木中 生長，藉此採取子實體。該種方法所得的牛樟芝子實體 $所含成分與野生種牛樟芝相似，“，該種栽培方式成 ^與時間均長，不利量產與推廣。特別是，牛樟芝椴木取 侍不易，使得牛樟芝相關產品價格高居不了。固體栽培法 主要是利用含有營養物質的太空包(亦即既有栽培香兹：方 法>’所得的牛樟芝其外觀與野生牛樟芝相似，然而，其三 200944120 +類化口物含量低，且該種栽培方式所得的牛樟芝子實體 主要成分與野生種牛樟芝子實體並不相同。此外，藉由接 ，牛樟芝g種進行液態發酵，雖然可在短時間内收成牛棒 之液體發酵產品，但是盆々 ,、 一疋/、主要產物為多醣類，三帖類化合 物卻無法取得，且與野生半工着^ 兴砰生午樟之子實體所含主要成份相去 甚遠。 有鐘於此，既有的牛樟芝人工栽培方法仍有不足 待進一步改良。200944120 IX. INSTRUCTIONS: [Technical field to which the invention belongs] This creation is related to the artificial cultivation method of Antrodia camphorata, and the special beta species does not need to use the conservation burdock as a cultivation medium. Artificial cultivation of bullets [Prior Art] Material: It is a precious fungus medicine that grows on the burdock tree of Taiwan's conservation tree species. Burdock * There are many medicines, among which the most important issues are the three-in-one combination research, the important chemical composition - the three-type compound makes the angustifolia: the bitterness of Antrodia camphorata, and the content of the three-stranded compound of Antrodia camphorata far exceeds spirit". The purpose of the three-type compound is to have anti-cancer, liver-protecting and lowering effects. In addition, other compounds contained in Antrodia camphorata, such as the multi-equivalent oxygen dismutase, '(4), η (4) physiologically active ingredients, are sufficient. A balanced health care function for human physiology. At 10 o'clock, the method of cultivation of the burdock is based on the use of the eucalyptus cultivation method, the solid cultivation method, and the liquid fermentation method. In the eucalyptus cultivation method, the eucalyptus eucalyptus is used to implant the burdock bacillus into the eucalyptus, and the burdock is grown in the eucalyptus, thereby taking the fruiting body. The method of the method has the same ingredients as the wild species of Antrodia camphorata. "This cultivation method has a long time and is unfavorable for mass production and promotion. In particular, it is not easy to take the burdock and arborvitae. The price of related products is high. The solid cultivation method mainly uses the space capsule containing nutrients (that is, the cultivated citron: method>', the appearance of burdock is similar to that of wild burdock, however, its three 200944120 + class mouth The content of the material is low, and the main component of the body of the Antrodia camphorata obtained by the cultivation method is not the same as the fruit body of the wild species of Antrodia camphorata. In addition, the liquid fermentation is carried out by picking up the seed of the Antrodia camphorata, although the bovine stick can be harvested in a short time. Liquid fermentation products, but pots, a cockroach /, the main products are polysaccharides, three kinds of compounds can not be obtained, and far from the wild semi-worked ^ Xing Yusheng Wuzi's main body contains a lot of ingredients. Therefore, the existing artificial cultivation method of Antrodia camphorata is still insufficient to be further improved.
【發明内容】 由於既有牛樟芝人工栽培方法無法提供簡易、不需牛 樟樹作為栽培介質、及富含三帖類化合物等優點，因此， 本發明主要目的在於提供一種新穎的牛樟芝人工栽培方 法，藉此以達到提供品質優良的牛樟芝。 為達成前述目的，本發明所提供的一種牛樟芝的人工 栽培方法’其係包含： 人提供適當培養I，該培養基係包含一種或一種以上包 含白米、小米、高梁或小麥的五穀雜糧’ 1。％至35%(v/v) 椰子水，根莖類作物、10%至3〇%(w/v)糖；其中， 五榖雜糧的比例約為彳升的培養基約使用〇 5至，5 公斤的五榖雜糧；根莖類作物的比例約為彳升的培養基約 使用1 0至50公克根莖類作物； 接種牛樟芝菌種，並使其於23.0〜27 〇<t的培養條件 下生長； ' 待培養基完全為牛樟芝菌種所覆蓋後，調整培養溫度 5 200944120 為28·0 32_〇c繼續培養至長出子實體；及 收取牛樟芝產物》 在本發明一較佳具體實施例中，五穀雜糧的比例為1 升的培養基使用〇·8至12公斤的五穀雜糧；較佳的是， i毅雜糧的比例為1升的培養基使用1公斤的五穀雜糧。 在本發明一較佳具體實施例中，根莖類作物的比例為 升的培養基使用15至30公克根莖類作物；較佳的是， 響 ❹ 根莖類作物的比例兔i 丨為1升的培養基使用20公克根莖類作 物0 本發月較佳具體實施例中，培養基中進而包含膠 冑原蛋白的量為]升的培養基約添加G刚公克 至2.0公克膠原蛋白； ^ ^ 蛋白，膠原蛋白的量為Π 含膠原 公克勝原蛋白。 升的培養基約添加。·5公克至 培養：本佳具體實施例中，已接種牛樟芝菌種的 Λ " 、； 5.0t的培養條件下生長，至培養基完全 為菌絲覆蓋後，調整培養溫度為30t。 2由前述技術手段，本發明所達成的功效在於·· 養源，而毋須使用昂貴:::=資材作為牛樟芝的營 時牛樟芝生長其可有效心貝=牛掉樹或牛樟樹椴木，同 要的是，栽培所得的產：：栽培時間與成本，更重 含量高，與野生種牛帖類與多塘體化合物 與推廣價值。 心，、、異，而更能提昇牛樟芝的應用 6 200944120 【實施方式】 本發明係相關於-種牛樟芝的人工栽培方法，主要係 藉由五穀雜糧提供牛樟芝生長所需的營養源’而可具有快 速培養與大量培養的優點’同時其主要具功效的三帖類化 合物與多醣體等成分’並不會因此減少。 專有名詞『適#培養基』意、指使牛樟芝培養於人工培 養基中，該人工培養基係適合牛樟芝生長，且足以維持其 藥理活性，例如含有一定量的三帖類化合物及多醣體等。 ® 本發明適合使㈣培養基種類含但不限於，富含殿粉 類的五穀雜糧，例如：白米、小米、高梁、小麥、豆類、 玉米等一種或一種以上的五縠雜糧，膠原蛋白、椰子水、 富含澱粉的塊根或塊莖類作物（根莖類作物），例如：馬鈐 薯、蕃薯、蓮藕等，及糖等；膠原蛋白的種類，包含但不 限於，取自魚皮、豬皮或牛皮等之膠原蛋白；使該等原料 溶解在1公升的水中’以作為牛樟芝的營養源。 其中，五穀雜糧的比例約為，配製i升的培養基約使 ® 用公斤的五榖雜糧，較佳係為配製]升的培養基 約使用0·8~1·2公斤的五穀雜糧，最佳係為配製’升的培 養基約使用1公斤的五榖雜糧；是需要可添加或不添加膠 原蛋白，膠原蛋白添加量為1升的培養基約添加〇 〇〇 iWO 公克膠原蛋白’較佳係為1升的培養基約使用0.5〜ι·5公 克膠原蛋白，最佳係為1升的培養基約使用彳公克膠原蛋 白；所使用富含殿粉的塊根或塊莖類作物比例，為1升的 培養基約使用1〇〜50公克塊根或塊莖類作物，較佳係為] 7 200944120 ^的培養基約使用15,公克塊根或塊莖類作物最佳係 升的培養基約使用20公克塊根或塊莖類作物 要可添加挪子水，添加比…升的培養基中約含有1〇: 至35%(V/V)椰子水，較佳係為1升的培養基約含有 (y/V)椰子水；糖的比例約為1升的培養基約使用10% 至3〇%(W/V)糖，較佳係為20%(W/V)糖。 所使用的牛樟芝菌種不限於既有的 新分離的牛樟芝菌種^ 次疋 ΦSUMMARY OF THE INVENTION Since the artificial cultivation method of Antrodia camphorata cannot provide simple, no need of burdock as a cultivation medium, and is rich in three-type compounds, the main object of the present invention is to provide a novel artificial cultivation method of Antrodia camphorata. This is to provide the quality of the Antrodia camphorata. In order to achieve the foregoing object, the present invention provides an artificial cultivation method for Antrodia camphorata, which comprises: providing a suitable culture I, the medium comprising one or more kinds of whole grains '1 containing white rice, millet, sorghum or wheat. % to 35% (v/v) coconut water, rhizome crops, 10% to 3% (w/v) sugar; among them, the proportion of glutinous grains is about 彳5 to 5 kg The amount of the five-grain crop; the proportion of the rhizome crop is about 10 to 50 grams of rhizome crops in the soaring medium; the strain is inoculated with the strain of Antrodia camphorata and grown under the culture condition of 23.0~27 〇<t; After the culture medium is completely covered by the strain of Antrodia camphorata, the culture temperature is adjusted to 5, 2009, and the culture is continued to grow to the growing fruiting body; and the product is collected from the Antrodia camphorata. In a preferred embodiment of the present invention, the whole grain is The ratio of 1 liter of medium is 8·8 to 12 kg of whole grains; preferably, 1 liter of medium is used in 1 liter of medium using 1 kg of whole grains. In a preferred embodiment of the present invention, the ratio of rhizomatous crops is 15 to 30 grams of rhizome crops in liter medium; preferably, the ratio of rhizome to rhizome is 1 liter of medium. 20 g of rhizome crops. In a preferred embodiment of the present month, the medium further contains a gadolinogen protein in a medium of about liters to about 2.0 g of collagen; ^ ^ protein, amount of collagen It is a collagen containing gram of protein. The liter medium is about added. · 5 gram to culture: In the specific embodiment of the present invention, it has been inoculated with Λ quot ",; 5.0t culture conditions, until the medium is completely covered with hyphae, the culture temperature is adjusted to 30t. 2 By the foregoing technical means, the effect achieved by the present invention lies in the maintenance of the source, and it is not necessary to use expensive:::=Materials as the camphor of Ox sinensis. The growth of O. chinensis can be effective for the heartbeat = cattle drop tree or burdock tree The same thing is that the yield of cultivation:: cultivation time and cost, higher content, and the value of wild type cattle and multi-tang compound and promotion. Heart,, and different, and more effective to enhance the application of Antrodia camphorata 6 200944120 [Embodiment] The present invention relates to an artificial cultivation method related to a species of Antrodia camphorata, mainly providing a nutrient source for growth of Antrodia camphorata by means of whole grains. However, it has the advantages of rapid culture and large-scale culture, and at the same time, its three-component compounds and polysaccharides, which are mainly effective, are not reduced. The proper term "suitable for the medium" means that the anthraquinone is cultivated in an artificial culture medium which is suitable for the growth of Antrodia camphorata and is sufficient to maintain its pharmacological activity, for example, containing a certain amount of three-label compound and polysaccharide. ® The present invention is suitable for the (4) medium type including, but not limited to, grain-rich grains, such as white rice, millet, sorghum, wheat, beans, corn, etc., one or more kinds of five grains, collagen, coconut water , starch-rich roots or tuberous crops (root crops), such as: horseshoe, potato, lotus root, etc., and sugar; collagen types, including but not limited to, from fish skin, pig skin or Collagen such as cowhide; dissolves these materials in 1 liter of water' as a nutrient source for Antrodia camphorata. Among them, the proportion of grains and grains is about the same, and the medium for formulating i liters is about to make kilograms of five grains of cereals, preferably for the preparation of the medium. The medium is about 0. 8~1. 2 kilograms of whole grains, the best system. About 1 kg of glutinous grains are used to prepare 'liter medium; it is required to add or not add collagen, and the amount of collagen added is 1 liter. About 〇〇〇iWO gram of collagen is added. The medium is about 0.5~1·5g of collagen, and the best one is about 1 liter of medium. About gram of collagen is used. The proportion of roots or tubers that are rich in temple powder is about 1 liter of medium. 〇~50g root or tuber crop, preferably ]7 200944120^The medium is about 15, the gram of roots or tubers is the best cultivating medium. About 20 grams of roots or tubers can be added. Water, adding more than 1 liter: to 35% (V / V) of coconut water, preferably 1 liter of medium containing about (y / V) coconut water; the ratio of sugar is about 1 liter The medium uses about 10% to 3% (w/v) sugar, Best lines of 20% (W / V) glucose. The strain of Antrodia camphorata used is not limited to the existing newly isolated strain of Antrodia camphorata.
牛樟芝菌種散發強烈黃樟香氣味，呈平板狀具黃橘色 且具有強烈味道。在料巾藥巾，牛樟芝通常作為解毒、 :肝、抗癌等用途。如同一般食用真菌或醫用蕈類，牛樟 之係具有多種營養物質，包含多醣體、三帖類、超氧化雙 歧酵素、維生素、蛋白質（免疫球蛋白)、微量元素（鈣、 碟及鍺等）、核酸、凝集素（agg|_in)、胺基酸、固醇 類、木質f (lignins)及其類似物等…般相信此些生物 活性成分可展現，抗腫瘤、增強免疫能力、抗過敏、抑制 血J、板凝集、抗病毒、抗菌、抗高血壓、降血糖、降膽固 醇、保肝等或類似功效。 三帖類為多數牛樟芝最廣被研究的成分。三帖類係為 -含有3 〇個碳原子的五環類（pentacycHc)或六環類 (hexacyclic)結構的化合物。 由於本發明所提供以五穀雜糧栽培牛樟芝的人工栽拉 方法’可大量且快速培養出富含三帖類及多醣類化合物， 皴人供栽培方法所得的成分物質，與野生種無異，因此， 8 200944120 本發明所使用的牛樟芝人工 進而可降低成本。 m，可有效用於量產， 牛圖所示’本發明的主要流程係藉由將部分 ) ’植菌在含有適當培養基的培養瓶 I 2 〇)内，而後經—適當時 出半棲-甚σ . 週田時間培養後，使培養基中生長 或為典：美：’ 5亥產品可為在培養基中佈滿菌絲的狀態， 乾燥：30、&長出子實體的狀態’將該培養基進行低溫 、 之後經磨粉已獲取牛樟芝產品（4 〇 ) » 為能詳細瞭解本創作的技術特徵及實用功效，並可依 照說明書的内容來實施， 明如後： ^步以較佳實㈣，詳細說 實施例 實施例一：牛樟芝的人工栽培方法 菌種來源： 既有任—種牛樟芝菌種。 培養基：The burdock strain has a strong yellow musk scent and is flat yellowish orange with a strong taste. In the towel wipes, Antrodia camphorata is usually used for detoxification, liver, and cancer. Like general edible fungi or medical mites, the burdock has a variety of nutrients, including polysaccharides, triads, superoxide dismutase, vitamins, proteins (immunoglobulins), trace elements (calcium, saucer and sputum). , nucleic acids, lectins (agg|_in), amino acids, sterols, wood f (lignins) and their analogues... I believe that these bioactive ingredients can exhibit anti-tumor, immune-enhancing, anti-allergic, It inhibits blood J, plate agglutination, antiviral, antibacterial, antihypertensive, hypoglycemic, cholesterol lowering, liver protection, etc. or similar effects. The three-poster is the most widely studied component of most cattle. The three-type is a compound of a pentacyclic cH or a hexacyclic structure containing 3 carbon atoms. Since the artificial planting method for cultivating Antrodia camphorata with whole grains can provide a large amount and rapid cultivation of a compound rich in triads and polysaccharides, the ingredients obtained by the method of cultivation are the same as those of wild species. , 8 200944120 The artificial method of the Antrodia camphorata used in the present invention can further reduce the cost. m, can be effectively used for mass production, the cow's diagram shows 'the main flow of the invention is by planting part of the 'in the culture bottle I 2 含有 containing the appropriate medium), and then - when appropriate - semi-habited - Σσ. After culturing in Zhoutian time, the medium is grown or used as a code: US: '5 hai products can be in the state of mycelium in the medium, dry: 30, & grows the state of the fruiting body' The culture medium is subjected to low temperature, and then the powder has been obtained from the Antrodia camphorata product (4 〇) » In order to understand the technical characteristics and practical effects of the creation in detail, and in accordance with the contents of the manual, it is as follows: ^Step to better (4) DETAILED DESCRIPTION OF THE EXAMPLES Example 1: Artificial cultivation method of Antrodia camphorata Source of the strain: There is a species of any species of Antrodia camphorata. Medium:
含有五穀雜糧的培養基。該培養基的成分，係為以i 升的水’加人1克膠原蛋白、25%(v/v>馬料、2Q%( 五威雜糧，其巾膠原蛋自來源，包含^限制，為來自魚 士或牛皮’五榖雜糧種類，包含但不限制，為白米、小米、 咼梁、小麥等任一種或一種以上的組合。 培養條件： 植菌後， 室培養約30 將含有菌種的培養基存放在25<>c左右的培養 天左右，至完全成熟待出菇時，意即培養基 9 200944120 • 完全為牛樟芝菌絲所覆蓋時，溫度則變為3〇°c，以刺激出 兹’再歷時約30天即可使培養基有效分解出三帖類及多 醣體（請參看附件一，其係經過搖瓶培養兩個月之情形）。 同時，所含的三帖類及多醣體與市售產品或野生牛樟芝子 實體無異。 加工條件： 將接種後經約60天培養的培養基取出，以低溫乾燥 方式使佈滿菌絲的培養基乾燥，經過研磨成粉後，則可獲 取所欲的牛樟芝成分。 實施例二：人工栽培之牛樟芝的成分分析 經由上述栽培方式所獲取的牛樟之子實體，委託財團 法人食品工業發展研究所進一步鑑定，所得鑑定結果為Medium containing whole grains. The composition of the medium is 1 liter of collagen in water, 25% (v/v > horse material, 2Q% (wuwei miscellaneous grains, and the collagen egg from the source, including ^ limit, from Fish or cowhide 'five grains type, including but not limited to, one or more combinations of white rice, millet, glutinous rice, wheat, etc. Culture conditions: After germination, room culture is about 30 medium containing strains Stored at about 25<>c around the culture day, until it is fully ripe for the mushroom, meaning medium 9 200944120 • When it is completely covered by the mycelium of A. glabrata, the temperature becomes 3〇°c to stimulate The medium can be effectively decomposed into three types and polysaccharides in about 30 days (please refer to Appendix I, which is shaken for two months). At the same time, the three types of polysaccharides and polysaccharides are included in the market. The product or the wild A. angustifolia fruit body is the same. Processing conditions: The medium cultured after about 60 days of inoculation is taken out, and the mycelium-cultured medium is dried by low-temperature drying method, and after grinding into powder, the desired content can be obtained. Antrodia camphorata ingredients. Two: Antrodia component analysis of artificial cultivation of fruiting bodies of Antrodia acquired via the cultivation methods, commissioned by Research Institute for Food Industry Development Consortium further identification obtained identification results
Ant「odia cinnamomea Τ·Τ· Chang & w. N· Chou。内容物 如下表所列： 表一、水解胺基酸含量表 樣品名稱 ------ iA芝子實體 Sample w.t% -^----- 菌絲體 S 3 ΓΠ π Ιο \λ/ + 〇/ 按基酸（Amino acid) 1 TAU牛績酸 0 -i σ W. 17〇 n 2 ASP天門冬胺酸 0.76 ----u 0 7C 3 THR息寧胺酸 0.41 --—* o 1 A7 4 S E R絲胺酸 0.49 ----L ·峙广 1 R1 5 GLU麩胺酸 0.85 ------- » O I 0 op 6 PRO脯胺酸 0.37 —'— (· o o 1 ςπ 7 GLY甘胺酸 0.40 ----*»o u ---1.37 200944120 8 ALA 胺基丙酸 0.51 1.95 9 CYS 胱胺酸 0.03 0.15 10 VAL 擷胺酸 0.38 1.70 11 MET 甲硫胺酸 0 0.22 12 ILE 異白胺酸 0.30 1.34 13 LEU 白胺酸 0.50 2.26 14 TYR 酥胺酸 0.46 1.13 15 PHE 苯胺基丙酸 0.73 1.39 16 HIS 組織胺酸 0.16 0.68 17 LYS 離胺酸 0.37 1.34 18 nh3 氨 1.24 2.76 19 ARG 魚精胺酸 0.41 2.09 表二 、游離胺基酸含量表 樣品名稱 牛樟芝子實體 牛樟芝菌絲體 胺基酸（Amino acid) Sample w.t% Sample w.t% 1 TAU 牛績酸 22.1 172 2 HYP 羥脯胺酸 0 28.0 3 THR 息寧胺酸 26.9 69.4 4 SER 絲胺酸 29.8 99.4 5 ASN 門冬醯胺 18.4 83.0 6 GLU 麩胺酸 26.0 83.4 7 GLN 麩胺醯胺 26.6 188 8 PRO 脯胺酸 10.2 72.1 11 200944120Ant "odia cinnamomea Τ·Τ· Chang & w. N· Chou. The contents are listed in the following table: Table 1. Hydrolyzed Amino Acid Content Table Sample Name ------ iA Chia Body Sample wt% -^ ----- Mycelium S 3 ΓΠ π Ιο \λ/ + 〇 / by amino acid (Amino acid) 1 TAU bovine acid 0 -i σ W. 17〇n 2 ASP aspartic acid 0.76 --- -u 0 7C 3 THR siminic acid 0.41 ---* o 1 A7 4 SER urine 0.49 ----L · 峙广 1 R1 5 GLU glutamic acid 0.85 ------- » OI 0 Op 6 PRO valine 0.37 —'— (· oo 1 ςπ 7 GLY glycine 0.40 ----*»ou ---1.37 200944120 8 ALA Alanine propionate 0.51 1.95 9 CYS Cystamine 0.03 0.15 10 VAL Proline 0.38 1.70 11 MET Methionine 0 0.22 12 ILE Isoleamic acid 0.30 1.34 13 LEU leucine 0.50 2.26 14 TYR lysine 0.46 1.13 15 PHE Anilinopropionic acid 0.73 1.39 16 HIS Histidine 0.16 0.68 17 LYS lysine 0.37 1.34 18 nh3 ammonia 1.24 2.76 19 ARG fish arginine 0.41 2.09 Table 2, free amino acid content table sample name Antrodia camphorata fruit body Astragalus membranaceus Amino acid Sample (%) Sample wt% 1 TAU Cattle acid 22.1 172 2 HYP Hydroxyproline 0 28.0 3 THR Glycine 26.9 69.4 4 SER Serine 29.8 99.4 5 ASN Aspartame 18.4 83.0 6 GLU Glutamine 26.0 83.4 7 GLN Glutenin amide 26.6 188 8 PRO proline 10.2 72.1 11 200944120
特徵的範圍内。 【圖式簡單說明】 第囷係為本發明的流程圖。 【附件說明】 附件一係為本發明牛樟芝經搖瓶培養兩個月之發酵情 形的照片。 【主要元件符號說明】 (1 0)牛樟芝接種源 (2 〇)含有適當培養基的培養瓶 (3 〇 )低溫乾燥 (4 〇 )牛樟芝產品 12Within the scope of the feature. BRIEF DESCRIPTION OF THE DRAWINGS The third embodiment is a flowchart of the present invention. [Description of Attachment] Attachment 1 is a photograph of the fermentation condition of the Antrodia camphorata cultured in shake flask for two months. [Explanation of main component symbols] (1 0) Inoculum of Antrodia camphorata (2 〇) Culture flask containing appropriate medium (3 〇) Low temperature drying (4 〇) Antrodia camphorata products 12