KR20050103889A - Method for germination and cultivation of plants using chaga mushroom and/or the extract - Google Patents

Abstract

The present invention relates to a method of allowing a large amount of beta glucan to accumulate in a plant by immersing in a chaga mushroom extract during germination of the plant, germinating by germinating, and fertilizing the chaga powder or extract thereof in the growing plant. In addition, it is possible to obtain an immune enhancing effect of beta glucan by simply ingesting a plant by a human. The present invention is characterized in that, first, the seeds are immersed in chaga extract to germinate, and second, the plants absorb the beta-glucan contained in chaga extract by fermenting chaga extract in solid or liquid to the plants that germinate and grow. It is characterized by that it accumulates itself. The present invention is characterized by providing a method for the beta-glucan to be contained in excess in the plant itself, and by simply ingesting the plant as a food intake at the same time beta-glucan contained in a large amount to help the immune system This can greatly contribute to the improvement of national health.

Description

Method for germination and cultivation of plants using Chaga mushroom and / or the extract}

The present invention immersed seeds in chaga extract, germinated, seeded, seeded, transplanted and cultivated in the process of cultivating again chaga mushroom powder or its extract in liquid or solid phase containing a large amount of beta glucan in the plant and its fruit It is characterized in that. Conventional techniques have been characterized in that the transplantation after germination, and to promote growth by foliar spraying of suitable fertilizers or fungicides, insecticides and the like, and to contain a large amount of a specific substance Jang Kwon Il It is unique to present a technique for cultivating high calcium-containing rice by foliar application of an ionized calcium solution prepared using the shell shown in 2004-0080047 as a powder. Kim Sang-eun proposes a technology characterized in that the soil is modified with a functional fertilizer composition containing sodium oxide and potassium oxide in Korean Patent Application No. 10-2003-0023647, Keimyung Christian Academy 10-2002- 0024054 proposes a method of increasing the immunity by foliar fertilization of microbial fertilizer using microorganisms and humic acid, and Yoon Seok-moon in Korea Patent Application 10-1999-0049627 describes a technique for preparing axal calcium fertilizer using wood vinegar and eggs and tangerine peel Suggesting.

However, these techniques are all aimed at promoting plant growth by simple foliar fertilization or preventing disease by increasing immunity.In the germination and growth process, the technique of increasing the accumulation of beta glucan in the plant or its fruits by fertilization It has not been introduced in any country.

Therefore, the present inventors have completed the present invention by developing a technique for containing a large amount of beta glucan in the plant obtained by administering chaga extract in the germination and growth stages as a method of increasing the content of beta glucan.

Ingatus (Inonotus obliquus) is the genus Basidiomycotina, Eubasidiomydetes, Hymenomycetidae, Aphyllophorales, Hynochachadeadeae Belongs to. Chaga can be found in alpine birch fields and is found in cold regions such as northern Japan, Russia and Mongolia. Chaga has long been used as a folk remedy for its pharmacological effects. The main pharmacological effects are gastric cancer and colorectal cancer and gastrointestinal cancer cells (Cha Jae Young et al., J. Korean Soc. Appl. Biol. Chem. 47 (2), 202-7, 2004; Cha Jae Young et al., J. Korean Soc. 33 (4), 633-40, 2004; Hwang, Yong-Ju et al., Korean Journal of Nutrition, 36 (1), 18-23, 2003) Inhibition of cell growth and glycemic control in diabetic patients (Ra Jung-chan et al., J. Korean Soc) food Sci.Nutr. 32 (4) 614-20, 2003) or antimutagenic activity (J. Korean Soc.food Sci.Nutr. 32 (7), 1088-94, 2003), antioxidant activity And genotoxic inhibitory effects (Ham Seung-si et al., J. Korean Soc. Food Sci. Nutr. 32 (7), 1071-75, 2003), transcription factors NF, which are known to play a major role in causative gene regulation in tumor, inflammation, and immune systems Effects on the inhibition of -kB activity (Song Hee-soon et al., Journal of Pharmacognosy, 35 (1), 92-7, 2004) have been reported. Lee Jong-hoon is a health food for preventing and treating diabetes in Korean patent application 10-2004-0018169, Hallym University is an anti-cholesterol composition in Korean Patent Application No. 10-2003-0057209, Bioneer is a Korean patent application 10-2003- In the method of manufacturing and processing mushroom mycelium at 0053565, Lee, Hyang-Beom is a method for preparing chaga mushroom extract composition for hypoglycemic and anti-inflammatory in Korean patent applications 10-2004-0008190, 10-2004-0008188, 10-2004-0008189, Doosan Corporation presents chaga mushroom composition for preventing prostate hyperplasia and constipation in Korean patent application 10-2002-0006062, and Kim Il-hwan presents chaga mushroom extract as animal feed composition in Korean patent application 10-2003-0033874. The Korean patent application 10-2003-0038973 is a supplement for preventing adult diseases and diabetes treatment, and Jaejae Green is a fermentation composition of chaga having anticancer activity in Korean patent application 10-2003-0065362 Hebentech has presented chaga mushroom composition as a gastritis treatment and anti-cancer, anti-inflammatory agent in Korean patent applications 10-2002-0021706, 10-2002-0021707, and Han Young-hwan has proposed Korean patent application 10-2003 -0073323 proposes the use of chaga extract as a cosmetic composition, ABL LL in Korea patent application 10-2005-000802 proposes a plant growth regulator using a mushroom culture medium containing chaga as an environmentally friendly agricultural supplement . In addition, beta glucan has been known to have an immune enhancing effect since the 1940s, and this immune enhancing effect is attributable to enhancing phagocytosis of macrophages when injected into a living body and is expected to be effective as an anticancer agent as well as sepsis treatment (Bohn, J). et.al., Carbohydrate polymers 28, 3-14, 1995). NC Biotech Co., Ltd. has proposed the use of mushroom mycelium as a functional feed additive for seawater fish, freshwater fish, and lobster in Korean patent application 10-2002-0072167. Until now, chaga extract has been mainly used for the treatment of diseases caused by the immune enhancing effect of human ingestion, and chaga for the purpose of accumulating beta glucan due to the germination and fertilization of plants pursued by the present invention. The use of mushroom extraction has not been reported.

Beta-glucan is bound to the polymer phase by using glucose as a basic unit, and classified into beta or alpha according to the binding form. Alpha glucan is a cellulose, starch, dextrin, etc., beta glucan is present in a small amount in the plant is known to be absorbed by the human body to enhance the immune function, the situation of beta glucan is known to have excellent anti-cancer activity.

The present invention is to immerse seeds in chaga extract, germinate, and so that the plants and their fruit by containing a large amount of beta glucan by fertilizing the chaga mushroom powder or its extract in a liquid or solid phase again. It is said that it is main contents of the technology.

An object of the present invention is to supply appropriate nutrients to plants when germinating and growing plants so that beta glucan accumulates in the plants. Therefore, the present invention is constructed as follows. First, the preparation of chaga extract, second, germination by immersion of seeds, third, fertilization of foliar fertilization or solid phase of the liquid phase at the appropriate interval during the growth of germinated sprouts to beta-glucan component by leaf or root It is absorbed and allowed to accumulate in large quantities in plants.

The present invention is based on the hypothesis that the organism including the plant remembers its surrounding environment during germination or the first growing season, germinates with a high content of betaglucan during germination, and then fertilizes it again during the growth process. The present invention relates to a method in which a plant receives beta glucan and accumulates in the body or fruit. In the present invention, since the beta glucan enhances the body's immune function to increase anticancer activity, the plant of the present invention can be used as an anticancer food. Chaga extract contains a large amount of beta glucan, the content of which is 80% to 90% (w / w) of the extract's solid content as a crude betaglucan based on the extract.

Hereinafter, the present invention will be described in detail.

Chaga mushroom extract of the first step is to cut the chaga mushroom and crushed to less than 5mm thickness, and then added to the water of 5 times to 30 times the weight of chaga, and extracted for 1 to 24 hours at 70 ~ 120 degrees Celsius . After extraction, the filtrate is separated by filtration, and 5 ~ 20 times of water is added to the remaining solid, and it is ground with a mixer, completely crushed, and extracted for 1 to 24 hours at 60 to 120 degrees Celsius. After extraction, the filtrate is filtered to remove solids. The resulting extract is a black liquid with a characteristic odor of chaga. Chaga mushroom extract thus obtained is used for seed germination and foliar fertilization. When extracting less than 5 times the water extraction, the extraction yield is economically low, and when higher than 30 times, the concentration of the extract is too low. In addition, when the extraction temperature is lower than 70 degrees, the extraction yield is significantly reduced, and if the extraction temperature is higher than 120 degrees, there may be component destruction due to high temperature. Extracting to a temperature higher than 100 degrees in the process of adding water means applying pressure. To be 120 degrees, the pressure of the container containing the extract should be 1 atm or higher. Applying pressure can also be applied when extracting below 100 degrees. In general, it is found that the extraction yield and the extraction efficiency are economical when maintaining the pressure of 0.9 at the relative pressure by applying air or nitrogen at 90 degrees. Extraction time can be extracted within 1 ~ 24 hours depending on the size of the pulverized particles, if less than 1 hour, the extraction yield is severely degraded, if longer than 24 hours will cause unnecessary time consumption and cost increase. The chaga extract obtained in this way has a solid content of 0.1% (wv) ~ 3.5% (w / v) and the solid content of chaga extract used for germination is 0.1 ~ 2.5% (w / v). If the concentration of the extract is higher than 2.5% (w / v), the viscosity of the liquid is increased, so that the fluidity is lowered when immersing seeds is not suitable. If the concentration of the extract is too high, after dilution with water to obtain a solid concentration of 0.1 ~ 2.5% (w / v). The remaining solids after extraction of the extract are blown into the water to increase the size, so it can be diluted with water and crushed by a mixer to be fertilized near the root of the plant.

The second step, immersion of seeds, differs in temperature and conditions depending on the type of seed. In the case of rice, barley and wheat, which are generally used as food, rice is sprouted, and then grows into a mosquito seed, and grows it by planting it, which is very suitable for applying the method of the present invention. First, treat rice seed with disinfectant to remove pathogens, spores, etc. buried in rice seed, and use salt water to screen poor rice seed generally at low temperature so that it has a specific gravity of about 1.08 ~ 1.15, and then add chaga extract The total chaga extract solid content is 0.1 ~ 2.5% (w / v), and then salt is added so that the final specific gravity is 1.08 ~ 1.15, which is the range generally used. . After dipping, remove excess liquid and germinate seeds in 30 degrees or more for 24 hours. Dab seeds germinated from chaga extract are transferred to the bed and grown until planted. Remove the plastic house before planting and fertilize Chaga mushroom extract with solid content of 0.1 ~ 1.5% (w / v). At this time, too high concentration of chaga extract may be deposited on the leaves to reduce photosynthesis, so excessive concentration should be avoided. 1 ~ 3 times a month after fertilization, especially during fertilization ~ 3 months, when rice grows vigorously, fertilize chaga extract with 0.1 ~ 1.5% (w / v). When the number of foliar fertilization is more than 4 times a month, it may cause excessive cost increase and may cause photosynthetic degradation due to foliar fertilized chaga extract, but the number of times is better within the limit that does not interfere with the growth of rice. The foliar fertilization period is until the flowers bloom and the ears run. In Korea, it is generally considered to be the end of August in the central region and to the middle of September in the southern region. In this way, the harvested rice has a beta-glucan content of 2 to 7 times higher than that of rice. This means that by simply ingesting rice, the immune system can be obtained by inducing beta glucan, and thus can contribute to improving farm household income and public health as functional rice. Since rice is cultivated in water, fertilization of solid phase is naturally extracted by paddy field, so there is no difference between foliar fertilization.

In the case of rice, the roots are always close to the water while the rice is growing, so it is possible to simply crush and fertilize chaga without extracting it with water. Crush the chaga with a pulverizer to make a fine powder, sift it with a 100 mesh eye sieve, re-pulverize larger ones, collect only what passes through a 100 mesh sieve, and dissolve it in water at a concentration of 0.2-10% (w / v) Evenly spread the chaga mushroom powder on rice by mixing and spraying, and the powder is sprinkled with the standing rice water and is extracted naturally to make the absorption of rice extract. It is possible. However, in this case, the efficiency is somewhat lowered because the extraction yield is low, but the chaga mushroom powder submerged in the water is gradually extracted, so that the chaga mushroom can be continuously absorbed through the roots of rice, so the sustainability is excellent. When spraying chaga powder, the concentration of powder below 0.2% (w / v) or above 10% (w / v) is disadvantageous in terms of efficiency, and outside this range does not mean that the effect is lost. . Furthermore, depending on the crop, spraying such as manure only powder without mixing with water can increase the content of beta glucan in the crop.

In the case of barley and wheat, germination does not germinate when sowing, but disinfects it with a disinfectant and sowing it directly into the paddy field. Thus, in the process of seed treatment for germination, 0.1 ~ 2.5% chaga extract is extracted as in the case of the rice seed. By soaking in water so as to be w / v), the barley and wheat harvested the following year can increase the content of beta glucan by 1.1 to 6 times. However, in the case of barley and wheat, foliar fertilization is rarely used, but in order to maximize the effect of the present invention, the first year after planting the chaga extract of solid content of 0.1-1.5% (w / v) Fertilize foliar 1 ~ 3 times a month for up to 1 month after flowering. The higher the number of foliar fertilization, the better the range does not interfere with the growth of plants due to the black color of chaga extract. However, foliar fertilization has a disadvantage in that it is cumbersome and requires a lot of hands, so the cost rises. As an alternative, chaga mushrooms can be powdered and fertilized in the same way as fertilizer. In this case, chaga is powdered and its particle size is 5 mm and fertilized in the form of a solid. In this case, when the particle size is smaller than 0.1mm, too many fine powders are blown during the fertilizing process, and when the particle size is larger than 5mm, the effective ingredient may take a long time to leak, which may reduce the effect.

Eggplant crops such as red pepper and eggplant are germinated by dipping in chaga extract 0.1 ~ 2.5% (w / v) at the germination stage and fertilized continuously in liquid or solid phase at the growth stage after germination. Particularly, in the stage of flowering and fertilization, the fruit is opened and the fruit grows, it is often fertilized in liquid or solid phase as soon as the flower blooms to open the fruit. The concentration of chaga mushroom extract in liquid foliar fertilization is 0.1 ~ 1.5% (w / v) 1 ~ 5 times per month foliar fertilization. The beta glucan content in the red pepper and eggplant harvested in this way was 3 to 11 times higher than the other cases, showing a very high beta glucan content. When fertilizing in solid phase, chaga powder is ground to a size of 0.1 ~ 5mm and fertilized at the root of individual crops. Fertilization is in excess of 0.5 to 50 g per individual abandonment, which does not interfere with crop growth. Too much, however, is a factor of cost increase in terms of economics and does not negatively affect the effect of the present invention.

The method of increasing the content of beta glucan in crops by germination and fertilization using chaga extract of the present invention can be applied to fruit. In general, the fruit is a perennial, so it can not be immersed in the germination stage can be applied only by solid phase or liquid fertilization method. Applicable fruits include grapes, apples, peaches and pears, and the increase in beta-glucan content is 1.1-7 times in grapes, 1.1-3 times in apples, 1.1-5 times in peaches, 1.1-3 in pears. It was a ship.

In the case of watermelon and melon, which belongs to the fruit family, it is applicable to germination and fertilization because it is a plant grown annually by germinating from seeds. In the case of watermelon and melon, germination and growth were carried out in the same manner as in the case of the above pepper, and the fruit was obtained. As a result, the content of beta glucan was 1.1-8 times higher than that of the conventional fruit, and 1.1-6 in melon. Fold increased.

In addition, it was also applicable to vegetables. In the case of vegetables, tomatoes, cabbage, carrots, potatoes, green onions, spinach, garlic, lettuce, sesame, perilla, radish, cabbage, onions, sweet potatoes, cucumber, pumpkin, broccoli, beans also apply vegetables of the present invention The content of beta glucan contained in the decorating site of was able to significantly increase. In the case of tomatoes and pumpkins, as in the case of pepper, the beta-glucan content of tomatoes, cucumbers and pumpkins could be increased by 1.1 to 6 times by immersion of seeds and foliar fertilization, and cabbage, leeks, spinach, cabbage, In the case of broccoli, the content of beta glucan was increased 1.2 ~ 4 times by immersion of seeds and foliar fertilization.In the case of potatoes, sweet potatoes, onions, and garlic, seeds were not easily immersed, which resulted in solid and liquid fertilization. In that case, it was possible to increase the content of beta glucan 1.1 ~ 4 times in the final edible portion.

As an additional effect of the present invention, in the case of rice, chaga mushroom extract of the present invention is foliar fertilization, compared with the case of no otherwise strong resistance to the need to apply a separate pesticides and fungicides can be an environmentally friendly organic method Got. The same effect was obtained in the case of red pepper and green onion, and the effect was relatively insignificant when the fruit was in the ground such as potatoes, sweet potatoes and onions.

Hereinafter, the present invention will be described in detail through the following examples. However, the scope of the present invention is not limited by the following examples.

Example 1 In case of dipping rice seed and foliar fertilization

          At the end of March, 100 g of rice seed was treated with disinfectant, and 300 ml of chaga extract with a solid content of 0.8% (w / v) was added to it, and salt was added to adjust the specific gravity to 1.12 and soak for 8 hours at 15 degrees Celsius. After the excess solution was poured out and germinated by treating it for 24 hours at 32 degrees Celsius. It was planted in nails prepared in advance and grown into a plastic house. At the end of May, the vinyl was peeled off, and chaga mushroom extract was foliar fertilized with a nebulizer at a solid concentration of 0.9% (w / v). Foliar fertilization was carried out twice a week while in the bed, and a total of four times before seeding. Planting was carried out in early June, and after planting, foliar application was done twice a month until the middle of September. The concentration of foliar fertilized chaga extract was 0.6% (w / v). The number of foliar fertilization after planting was 7 times. The ripened rice was harvested in early October and the contents of beta glucan were analyzed and compared. The analysis of beta glucan is the primary method of crushing harvested rice seed, extracted with water at 90 ° C, filtered to remove solids, and then concentrated to reduce the volume to 1/10, and then ethanol is added to ethanol The concentration of is 90%, and then the precipitated material is recovered by centrifugation. The precipitate is dissolved in water and filtered. The filtrate is concentrated to 1/10, and then ethanol is added to the precipitated material. After drying, the weight thereof was measured. In order to compare the content of beta glucan, relative comparison was made based on rice seed not subjected to the dipping and foliar fertilization process of the present invention. As a result, the sample of the present invention was found to be more than 5 times the content of beta glucan compared to the reference sample.

       Comparison of Beta Glucan Content in Rice Seeds sample Beta Glucan Content (%, w / w) Sample of the Invention 3.5 Sample 0.68

The method in Table 1 compares the content of Crude Beta Glucan, so the results were compared through a more precise analysis.

The analysis method is as follows.

1) 10% of 2% caustic soda solution is added and reacted at 90 ° for 5 hours. After cooling, the supernatant is recovered by centrifugation (3000g, 10 minutes).

2) The supernatant is neutralized with 2M acetic acid and 3 volumes of ethanol are added and left for 24 hours to recover the precipitate. The precipitate is obtained by washing three times with ethanol: water = 3: 1 (v / v) and centrifuging.

3) The recovered precipitate was treated with 3% acetic acid at 0.1 g / ml (w / v) and then centrifuged to obtain a supernatant and neutralized with 2M caustic soda.

4) Eluate with NaCl (0-1M) in 0.01M Tris buffer (pH 8.0) by DEAE-ion exchange chromatography. In this process, the mannan protein is removed.

5) The sample recovered in the process of 4) was precipitated with 90% ethanol to recover beta glucan, dried and the weight thereof was measured.

The results are shown in the following table.

       Comparison of Purified Beta Glucan Content sample Beta Glucan Content (%, w / w) Sample of the Invention 2.3 Sample 0.4

As a result, the sample of the present invention showed a beta glucan content of 5.9 times, it was confirmed that the content of the beta glucan significantly increased.

Example 2. Comparison of Beta Glucan Content in Red Pepper Seeds

Red pepper seeds were immersed in the same manner as in Example 1, and the seeds were grown by direct seeding. As in the case of rice seedlings, when fertilized and grown more than 3cm, 0.7% (w / v) chaga extract was foliarly fertilized twice a week. After planting the seedlings were foliar fertilized twice a month. In addition, 2 g each was fertilized within a radius of 5Cm around the roots, as the chaga mushroom powder was passed through 50 mesh after each aeration. In addition, the solids prepared chaga extract was completely crushed with a mixer and put it in water and fertilized at the root of the red pepper. After the red pepper was opened, the red pepper was harvested in the state of green pepper, and the content of zbetaglucan was measured in the same manner as in Example 1. The results are shown in the following table.

        Comparison of Zobetaglucan Content in Red Pepper sample Zobeta carotene content (%, w / w) Sample of the Invention 1.2 Sample 0.14

As a result, the sample of the present invention was 8 times higher in the content of jobetaglucan than the reference sample.

Example 3. Comparison of Beta-Glucan Content When Treated with Garlic

Garlic is usually sliced and straight cut into whole garlic. Garlic was soaked in 0.6% (w / v) chaga extract before sowing, germinated and sown. After germination, when the sprouts of garlic grew more than 3Cm, the chaga mushroom extract of 0.7% (w / v) was applied once a month. In addition to this, only chaga mushroom powdered and screened through a sieve of 30 mesh was applied once a month at a concentration of 10 g / m ^ 2 per garlic growing area. As shown in Table 4, the beta glucan content of the harvested garlic showed a beta glucan content of 3.2 times higher than the standard sample.

 Comparison of Contents of Zobetaglucan in Garlic sample Zobetaglucan content (%, w / w) Sample of the Invention 1.2 Sample 0.31

Example 4 Effects on Other Crops

Barley, wheat, eggplant, cucumber, pumpkin, broccoli, beans, apples, grapes, pears, peaches, tomatoes, cabbage, carrots, potatoes, green onions, spinach, radish, cabbage When seeding seeds, onions, sweet potatoes, melons, watermelons, perilla, sesame seeds, lettuce, treated and germinated as in Example 1, seeding, foliar fertilization and powder fertilization, apples, pears, grapes, peaches, etc. In the case of perennial trees as in the fruit tree of the fertilization method was the same as the fertilization method of Example 2 powder fertilization and foliar fertilization. As a result, the beta glucan content of the edible portion used for food was shown in the following table.

Comparison of Zobetaglucan Content in Different Crops (%, w / w) sample barley wheat Branch cucumber pumpkin Broccoli bean tomato cabbage Sample of the Invention 6.3 5.3 1.4 0.7 2.2 5.3 1.9 1.9 2.4 Sample 1.1 2.1 0.3 0.2 0.9 1.3 1.1 0.7 1.5

sample carrot potato wave spinach radish cabbage onion sweet potato Sesame Sample of the Invention 2.1 0.6 0.5 0.7 0.12 0.34 0.99 1.2 1.9 Sample 1.0 0.4 0.2 0.1 0.1 0.2 0.6 0.9 0.9

sample Perilla Lettuce Apple grape ship peach Sample of the Invention 2.2 1.9 1.2 1.8 1.5 2.1 Sample 1.5 1.2 0.5 0.9 1.1 0.7

As can be seen in Table 5 above, the method of the present invention was able to obtain significant results that can significantly increase the content of beta glucan in most edible crops and fruit trees.

In addition to the increase in beta glucan, lettuce, spinach, Chinese cabbage, cucumber, tomato, etc., the growth of the plant was vigorous, less pests, and the ratio of fruit to fruit was relatively small. In the case of lettuce and spinach, the growth rate was short, and the time to the final harvest time was shortened, and the plant height was increased by 20 ~ 35%.

The present invention relates to a method for significantly increasing the content of beta glucan contained in the harvest (edible part) obtained by fermenting chaga mushroom extract in fermented germination and / or liquid or solid phase in fruits, vegetables and grains. As the content of glucan increases, the intake of beta-glucan, which has an immune enhancing effect by the simple intake of food, can increase the prevention and defense against various diseases, and beta-glucan is recognized as an anti-cancer food and suffers from cancer. By providing easy anti-cancer food to people, it can make a great contribution to the health and health promotion of the people, and can contribute to the income increase of farmers by producing functional vegetables, fruits and grains.

Claims (4)

Plants are grown by immersing and germinating plant seeds with Chaga extract as a main ingredient, and fertilize chaga or its extract in liquid or solid state, or fertilize chaga or its extract in liquid or solid phase in previously grown plants. Method for increasing the content of beta glucan of the decorative part of the plant obtained by the method. Chaga mushroom extract used for immersion of seeds in claim 1 is obtained by adding 5 to 30 times the water to chaga mushroom, extracted by 1 to 24 hours at 70-120 degrees Celsius, the content of solids is 0.1 for germination ˜2.5% (w / v), the foliar cost is a method characterized in that the content of solids is 0.1 ~ 1.5% (w / v). As a plant in claim 1, rice, barley, wheat, pepper, eggplant, cucumber, pumpkin, broccoli, soybean, apple, grape, pear, peach, tomato, cabbage, carrot, potato, leek, spinach, garlic, radish, cabbage, Method comprising the onion, sweet potato, watermelon, melon, sesame, perilla, lettuce, etc. When the fertilization in the solid phase in claim 1, the size of chaga powder is characterized in that less than 5mm.