CN106550764A - A kind of SOYBEAN IN HIGH-YIELD BREEDING method of Cordyceps sinensis - Google Patents
A kind of SOYBEAN IN HIGH-YIELD BREEDING method of Cordyceps sinensis Download PDFInfo
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- CN106550764A CN106550764A CN201510634134.3A CN201510634134A CN106550764A CN 106550764 A CN106550764 A CN 106550764A CN 201510634134 A CN201510634134 A CN 201510634134A CN 106550764 A CN106550764 A CN 106550764A
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Abstract
The present invention is a kind of SOYBEAN IN HIGH-YIELD BREEDING method of Cordyceps sinensis, and biomedicine field plants a kind of method of medicinal material, having on present society to larva carries out daily 2 time in Chan Chong areas with sprinkler by aweto strain liquid, the sprinkling of 3 times altogether, Chan Chong areas bat moth larvae can not be put together again, preferably go out 2 excellent instar larvaes of sturdy obesity.For the problem, the present invention is sprayed at excellent species liquid on mulberry leaf, the silkworm of stable breeding is allowed to eat, when the silkworm of Deng Chanchong areas bat moth larvae and stable breeding grew to for 2 age, preferably go out 2 excellent age silkworm larvaes of sturdy obesity in the silkworm of stable breeding and excellent species liquid is sprayed to which, then in Chan Chong areas by per 1m2Put 52 excellent age silkworm larvaes, carry out naturally infection diffusion, adjacent larva mutually infects, mycelium is formed as early as possible in polypide, spore is sprouted after invading living worm body, worm is pierced in soil, forms sclerotium in very long winter, and sclerotium front end is born stroma again and passes Zinc fractions little Hua i.e. ascus ascospore.
Description
First, technical field：The present invention is a kind of SOYBEAN IN HIGH-YIELD BREEDING method of Cordyceps sinensis, is a kind of breeding method of plantation medicinal material in biomedicine field.
2nd, background technology：The Cordyceps sinensis breeding method adopted on present society is：Spore suspension and strain separating and pure medium liquid are configured to into strain liquid jointly and load sprayer, then daily 2 times are carried out to 2 instar larvae of worm area, strain liquid sprinkling in 3 days altogether, unfortunately worm area is sprayed 6 times, the excellent species liquid for wasting valuable rareness cannot also be concentrated and preferentially select fat sturdy 2 excellent instar larvaes which is sprayed.
3rd, the content of the invention, in order to solve above deficiency problem, therefore invented a kind of SOYBEAN IN HIGH-YIELD BREEDING method of Cordyceps sinensis：With excellent strain liquid sprinkling mulberry leaf, the silkworm of stable breeding is allowed to eat, when Deng Chanchong areas bat moth grew to for 2 age with stable breeding silkworm, preferably go out 2 excellent age silkworm larvaes of sturdy obesity in stable breeding silkworm carries out the sprinkling of excellent species liquid, the excellent Cordyceps sinensis of the sturdy obesity of output to which.
4th,Accompanying drawingExplanation：Figure 1It is, from the Cordyceps sinensis for newly digging out, to pick out the Cordyceps sinensis of the sturdy no disease and pests harm of physique, obtains the spore in the shell of ascus on cordyceps militaris sporocarp, the spore suspension for making 5%-10% concentration is separated with cordyceps species and pure medium：10 grams of many property peptones、30 grams of glucose、1 gram of yeast extract、0.55 gram of magnesium sulfate、1 gram of potassium dihydrogen phosphate、20 grams of agar、5 milliliters of auxin adds water 1000 milliliters，It is made into jointly excellent strain liquid，Load injector，First excellent species liquid is injected on mulberry leaf，Stable breeding silkworm is allowed to eat，When Deng Chanchong areas bat moth larvae grew to for 2 age with stable breeding silkworm larvae，The fresh and alive excellent larva of sturdy obesity is selected from stable breeding silkworm，Put together carries out the sprinkling of excellent species liquid to which，Then 5 outstanding 2 age silkworm larvaes are put by each square metre in Chan Chong areas，Excellent 2 age silkworm larvae is allowed to spread in Chan Chong areas self-assembling formation，Adjacent larva mutually infects，Mycelium is formed as early as possible in larva body，Spore is sprouted after invading larva live body、Worm is pierced in soil，Sclerotium is formed in very long winter，A kind of high yield Cordyceps sinensis that stroma passes Zinc fractions little Hua is born again in sclerotium front endFigure。Figure 2-1Pierce polypide in heaven and head in soil and grow sclerotiumFigure。Figure 2-2It is ascus ascospore that stroma passes Zinc fractions little HuaFigure。
5th, specific embodiment：The present invention is a kind of SOYBEAN IN HIGH-YIELD BREEDING method of Cordyceps sinensis, and simple to operate easy to learn, the cost of material is low, is easily promoted.
Claims (1)
1. a kind of SOYBEAN IN HIGH-YIELD BREEDING method of Cordyceps sinensis of present subject matter title, its feature are claimed
It is：The Cordyceps sinensis of the sturdy no disease and pests harm of physique from the Cordyceps sinensis for newly digging out, is picked out, Chinese caterpillar fungus is obtained
Spore in fructification in the shell of ascus, the spore suspension for making 5%-10% concentration are separated with cordyceps species and pure
Change culture medium：10 grams of many property peptones, 30 grams of glucose, 1 gram of yeast extract, thin 0.55 gram of magnesium of acid, sulfuric acid two
Hydrogen first, 1 gram, 20 grams of agar, 5 milligrams of auxin, 1000 milliliters are added water, be made into jointly excellent species liquid, first
Excellent species liquid is sprayed on mulberry leaf, allows stable breeding silkworm to eat, Deng Chanchong areas bat moth larvae is young with stable breeding silkworm
When worm grew to for 2 age, then the fresh and alive excellent silkworm larvae of sturdy obesity is picked out from 2 instar larvae of stable breeding silkworm,
Put together carries out the sprinkling of excellent species liquid to which, and then 5 outstanding 2 are put by each square metre in Chan Chong areas
Age silkworm larvae, allows excellent silkworm larvae to spread in Chan Chong areas self-assembling formation, and adjacent larva mutually infects, larva
Form mycelium in vivo as early as possible, sprout after the live body of spore 2 instar larvaes of intrusion, worm pierces (1) in soil, very long
Winter formed sclerotium (2), the front end of sclerotium is born stroma again and passes Zinc fractions little Hua i.e. ascus and ascus spore
Sub (3).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510634134.3A CN106550764A (en) | 2015-09-30 | 2015-09-30 | A kind of SOYBEAN IN HIGH-YIELD BREEDING method of Cordyceps sinensis |
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| CN106550764A true CN106550764A (en) | 2017-04-05 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108496694A (en) * | 2018-03-28 | 2018-09-07 | 全家百（苏州）生物科技有限公司 | By generative propagation strain by silkworm cultivation at the method for silkworm grass |
| CN108739044A (en) * | 2017-12-29 | 2018-11-06 | 谢远泰 | A kind of silkworm larva infection parent species bacterial strain and artificial cultivating method for north Chinese caterpillar fungus |
| CN109566269A (en) * | 2018-12-13 | 2019-04-05 | 湖州柳荫生物科技有限公司 | A kind of culture medium improving cordycepin content in Cordyceps militaris |
| CN109618811A (en) * | 2018-12-25 | 2019-04-16 | 长兴制药股份有限公司 | Industrialization cordyceps sinensis artificial culturing method |
Citations (5)
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| JPH11103666A (en) * | 1997-10-07 | 1999-04-20 | Yoshii Kingaku Kenkyusho:Kk | Culture of cordyceps sinensis sacc. |
| CN1970734A (en) * | 2006-12-04 | 2007-05-30 | 中山大学 | Method for producing aweto |
| CN101707966A (en) * | 2009-04-14 | 2010-05-19 | 徐正华 | Living Chinese caterpillar fungi artificially cultivated by using larvae as hosts and method for cultivating same |
| CN102792855A (en) * | 2012-09-13 | 2012-11-28 | 重庆市中药研究院 | Cordyceps sinensis host infection strain material and method |
| CN103299821A (en) * | 2012-03-08 | 2013-09-18 | 黄友鹰 | Original ecological cordyceps sinensis high yielding and breeding method |
-
2015
- 2015-09-30 CN CN201510634134.3A patent/CN106550764A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11103666A (en) * | 1997-10-07 | 1999-04-20 | Yoshii Kingaku Kenkyusho:Kk | Culture of cordyceps sinensis sacc. |
| CN1970734A (en) * | 2006-12-04 | 2007-05-30 | 中山大学 | Method for producing aweto |
| CN101707966A (en) * | 2009-04-14 | 2010-05-19 | 徐正华 | Living Chinese caterpillar fungi artificially cultivated by using larvae as hosts and method for cultivating same |
| CN103299821A (en) * | 2012-03-08 | 2013-09-18 | 黄友鹰 | Original ecological cordyceps sinensis high yielding and breeding method |
| CN102792855A (en) * | 2012-09-13 | 2012-11-28 | 重庆市中药研究院 | Cordyceps sinensis host infection strain material and method |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108739044A (en) * | 2017-12-29 | 2018-11-06 | 谢远泰 | A kind of silkworm larva infection parent species bacterial strain and artificial cultivating method for north Chinese caterpillar fungus |
| CN108496694A (en) * | 2018-03-28 | 2018-09-07 | 全家百（苏州）生物科技有限公司 | By generative propagation strain by silkworm cultivation at the method for silkworm grass |
| CN109566269A (en) * | 2018-12-13 | 2019-04-05 | 湖州柳荫生物科技有限公司 | A kind of culture medium improving cordycepin content in Cordyceps militaris |
| CN109618811A (en) * | 2018-12-25 | 2019-04-16 | 长兴制药股份有限公司 | Industrialization cordyceps sinensis artificial culturing method |
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