CN109370914A

CN109370914A - A kind of cordyceps sinensis mycelium powder and its production method

Info

Publication number: CN109370914A
Application number: CN201811431180.3A
Authority: CN
Inventors: 马兰; 陈大伟; 刘小朋
Original assignee: Individual
Current assignee: Individual
Priority date: 2018-11-28
Filing date: 2018-11-28
Publication date: 2019-02-22
Anticipated expiration: 2038-11-28
Also published as: CN109370914B

Abstract

The present invention relates to technical field of biological fermentation, disclose a kind of aweto Ophiocordyceps sinesis mycelium powder and its production method, specifically using aweto as strain, selenium-rich aweto mycelium is produced by adding inorganic selenium or/and organic selenium liquid deep layer fermenting in the medium, dry mycelial yield 28-35g/L, mycelium includes total selenium 25-1350ug/g, organic selenium accounts for total selenium mass percent w/% > 92%, also contain cordycepic acid 4-8% in two kinds of mycelium, Cordyceps sinensis polysaccharide 5-8%, the effective components such as adenosine 0.03-0.17%, mycelium powder is broken to 80-100 mesh, mycelium powder finished product is made.Experimental study proves, this product has the function of anti-oxidant, anti-cancer and cancer-preventing, antifatigue, strengthen immunity, the giving young employees remedial-courses in general knowledge and vocational skills of lung kidney, protecting liver and detoxication etc., no matter as the nutritional supplement of drug, health food and selenium all has practicability.

Description

A kind of cordyceps sinensis mycelium powder and its production method

Technical field

The present invention relates to technical field of biological fermentation, and in particular to a kind of selenium-rich cordyceps sinensis of liquid deep layer fermenting production Mycelium powder and its production method.

Background technique

Cordyceps sinensis (Cordyceps sinesis) lives in Qinghai-Tibet height above sea level 3000-5200 meters of Alpine meadows, is one Kind entomogenous fungi complex.Its entomogenous fungi relationship is that aweto (Ophiocordyceps sinesis) infects Hepialidae elder brother After the larva of worm, the intracorporal nutrition of larva body is gradated as mycelium full of entire body tissue, one layer of worm skin is only remained, It keeps worm shape constant in low temperature drying soil in winter and is known as winter worm up to the several months long, on polypide head when summer is warm and humid suitable Grow clava (stroma) and the referred to as summer grass that bassets.Aweto also once uses Hirsutella hepiali Chen et Shen (Hirsutella hepiali chen etshen), Hirsutella sinensis (Hirsutella sinensis), aweto-cephalosporin (Cepha losporium dongchongxiacaonia), Chinese synnema spore (Synnematium sinense) are named, these All it is homonymus or synonymum, has uniformly been changed to aweto (Ophiocordyceps sinesis).

Cordyceps sinensis, which has, adjusts yin-yang, the special efficacy of lung kidney giving young employees remedial-courses in general knowledge and vocational skills, is the Chinese medicine that can uniquely balance, adjust yin-yang simultaneously. Modern pharmacology it has proven convenient that cordyceps sinensis have immunological regulation, antitumor, anti-oxidant, anti-aging, antifatigue, anti allergic reaction, Reducing blood sugar and blood lipid and gonadotropic Effect etc..So aweto is by state food drug surveilance office with bat moth coat Spore name is included in " can be used for health care product fungi strain list " (prevailing for the time being in force).

Selenium is the essential trace elements of the human body, referred to as " life special effect element ".It is numerous studies have shown that selenium has anti-cancer anti- The effects of cancer, strengthen immunity is anti-oxidant, prevents cardiovascular and cerebrovascular disease, detoxifies heavy metal element, toxin expelling, protects liver.Selenium It is the important component of a variety of enzymes of human body.Currently, selenium-rich product is divided into two class of inorganic selenium-rich and organic Se-rich.The poison of inorganic selenium Property be greater than organic selenium, activity again be lower than organic selenium.Inorganic selenium-rich product main representative is sodium selenite, organic selenium-rich product Selenium compound product, microorganism selenium-rich product, plant selenium-rich product, four class of animal selenium-rich product can be divided into.

It is strain that the present invention, which is with aweto, is sent out by adding inorganic selenium or organic selenium deep liquid in the medium Ferment produces selenium-rich cordyceps sinensis mycelium powder.

Since aweto is extremely stringent to temperature requirement, suitable growth temperature be 17-20 DEG C, temperature be more than 22 DEG C just Its growth is seriously affected, temperature is more than 25 DEG C and even causes death；Moreover, aweto slow growth, deadtime are longer.? The prior art fermentation period of report is up to 15-20 days, and mycelial yield is higher to be reported as the 20.8g/L (fermentation such as Wang Yingyan Science and technology communication the 43rd phase Hirsutella sinensis present Research July in 2014 and development trend).Fermenting and producing selenium-rich Chinese caterpillar fungus bacterial filament Research achievement in terms of body is less.Zhou Jianqiao etc. uses aweto for selenium-rich carrier, and brewex's grains are matrix liquid deep layer hair Ferment, the total selenium-rich amount of cordyceps sinensis are 5808.20 μ g/L (mycelia dry mass × Se content), and dry mycelia yield is 12.28g/L, and conversion is dry The total selenium amount of mycelia is 472 μ g/g (" China's brewing " 2014).Chen Hongwei etc. is with the selenium-enriched aweto of plasm mutagenic and breeding Strain, total Se content is up to 125.8 μ g/g, thallus yield 13.445g/L (" food and fermentation industries " 2009).Hu Min etc. uses winter worm Summer grass bacterium carries out selenium-enriched liquid fermentation, thallus yield 2.52%, Se content 7.90mg/Kg (Hubei University's journal 2006.28).This The Chinese patent of aspect is also few, and CN201510992589.2 discloses a kind of rice bran liquid fermentation aweto mycelium, bacterium Silk Enriching Selenium 32.97 μ g/g, mycelia yield 4.55g/L.CN201610579616.8 discloses the winter worm of a kind of low lead of selenium-rich, arsenic The summer mycelial culture medium of grass and its inoculation method, 52.01 μ g/Kg of Se content in the mycelium of production, mycelial yield are not done Description.

In the prior art, submerged fermentation selenium-rich aweto mycelium yield is all lower, while there is also inorganic selenium benefits Defect lower with rate, that product Se content is lower and organic degree is not high.So there is presently no liquid deep layer fermentings The report of selenium-rich cordyceps sinensis mycelium powder industrialization.Applicant passes through to the screening of culture medium and the improvement of cultural method, gram The defect for having taken the prior art achieves selenium-rich aweto mycelium yield 35g/L, contains total selenium 1350ug/g, wherein organic Selenium accounts for 92% or more of total selenium amount, the research achievement of inorganic 85% or more se use efficiency.

Summary of the invention

The object of the present invention is to provide a kind of cordyceps sinensis mycelium powder and its production methods.Specifically with aweto For strain, selenium-rich aweto mycelium is produced by adding inorganic selenium or/and organic selenium liquid deep layer fermenting in the medium Powder, dry mycelial yield 28-35g/L, dry mycelium include total selenium 25-1350ug/g, organic 92% or more rate, in culture medium 85% or more the utilization rate of inorganic selenium；Also contain cordycepic acid 4-8%, Cordyceps sinensis polysaccharide 5-8%, adenosine 0.03- in dry mycelium 0.17%.The mycelium powder is broken into 80-100 mesh and is prepared into selenium-rich cordyceps sinensis mycelium powder.

Aweto (Ophiocordyceps sinesis) strain of the invention is selected from Research for Industrial Microbial Germ preservation Administrative center CICC, number 14088.

The present invention is achieved by the following technical solutions:

A kind of fermented cordyceps sinensis mycelia powder, the cordyceps sinensis mycelium powder are passed through using aweto as strain Sodium selenite or/and Se-enriched yeast liquid Submerged fermentation are added in the medium, and fermentation liquid is by separation of solid and liquid and drying Process, the dry mycelium of gained include total selenium 25-1350ug/g, and wherein organic selenium is not less than 92%；The mycelium powder is broken into 80- Mycelium powder is made in 100 mesh, and mycelium powder is powdered to yellowish-brown in lark, has micro- fishy smell free from extraneous odour, takes a small amount of plus Asia After the dyeing of first indigo plant, set that oil mirror microscopy under microscope is visible to be had every multicore mycelia.

A kind of fermented cordyceps sinensis mycelia powder, the cordyceps sinensis mycelium powder are passed through using aweto as strain Sodium selenite or/and Se-enriched yeast liquid Submerged fermentation are added in the medium, and fermentation liquid is by separation of solid and liquid and drying Process, the dry mycelium of gained include total selenium 25-1350ug/g, and wherein organic selenium is not less than 92%, also containing the cordyceps sinensis of 7-8% Acid, the adenosine of 5-8% Cordyceps sinensis polysaccharide and 0.03-0.17%；The mycelium powder is broken into 80-100 mesh, mycelium powder finished product is made, Finished product has micro- fishy smell free from extraneous odour, takes this product a small amount of in lark to yellowish-brown, after adding methylene blue staining, sets oily under microscope Mirror microscopy is visible to be had every multicore mycelia.

A kind of fermented cordyceps sinensis mycelia powder, the cordyceps sinensis mycelium powder are passed through using aweto as strain Sodium selenite or/and Se-enriched yeast liquid Submerged fermentation are added in the medium, and fermentation liquid is by separation of solid and liquid and drying Process, the dry mycelium of gained include total selenium 500-1350ug/g, and wherein organic selenium is not less than 92%, also containing the cordyceps sinensis of 7-8% The mycelium powder is broken into 80-100 mesh and mycelium powder finished product is made by acid, the adenosine of 5-8% Cordyceps sinensis polysaccharide and 0.03-0.17%, Finished product is powdered to yellowish-brown in lark, has micro- fishy smell, free from extraneous odour takes this product a small amount of, after adding methylene blue staining, sets micro- Oil mirror microscopy is visible under mirror has every multicore mycelia.

A kind of fermented cordyceps sinensis mycelia powder, the cordyceps sinensis mycelium powder are passed through using aweto as strain Sodium selenite or/and Se-enriched yeast liquid Submerged fermentation are added in the medium, fermentation liquid passes through separation of solid and liquid and drying, The dry mycelium of gained includes total selenium 1000-1350ug/g, and wherein organic selenium is not less than 92%, also containing the cordycepic acid of 7-8%, 5- The mycelium powder is broken into 80-100 mesh and mycelium powder finished product, finished product is made by the adenosine of 8% Cordyceps sinensis polysaccharide and 0.03-0.17% Physical behavior has micro- fishy smell in lark to yellowish-brown, and free from extraneous odour takes this product a small amount of, after adding methylene blue staining, sets microscope Lower oil mirror microscopy is visible to be had every multicore mycelia.

The cultural method of above-described cordyceps sinensis mycelium powder, comprising: slant strains culture, shake-flask seed culture, First class seed pot culture, secondary seed tank culture, fermentation tank culture are separated by solid-liquid separation, dry, detection, the technical process of crushing.Tool Steps are as follows for body:

(1) slant strains culture

Slant strains culture selects Chinese industrial Microbiological Culture Collection administrative center DCH culture medium, by weight volume ratio (%) meter: sucrose 5.0%, yeast powder 0.3%, tryptone 1.0%, agar powder 1.0%, inorganic ionic solution pH 5.6-6.0 (0.5 gram of magnesium sulfate, 1 gram of potassium dihydrogen phosphate, 1 gram of sodium chloride, 0.3 gram of calcium chloride, 0.01 gram of manganese sulfate, 0.01 gram of zinc sulfate, sulphur Sour ferrous iron 0.01 gram, 0.01 gram of copper sulphate, 1000 milliliters of distilled water constant volume).121 DEG C of sterilizings after packing, incline inclined-plane processed, will pass through The strain of activation is inoculated in slant medium, 15-18 DEG C culture 30-40 days.

(2) shake-flask seed culture

Shake flask medium: volume ratio (%) is counted by weight: glucose 1.0-2.0%, corn flour 1.0-2.5%, molasses 0.5-1.5%, potato 1.0-6.0%；Dried silkworm chrysalis meal 0-3.0%, silkworm chrysalis 0-6.0% living, soybean powder 0-1.5%, yeast powder 0.2-1.0%, wheat bran 0.5-1.5%；Magnesium sulfate 0.01-0.05%, potassium dihydrogen phosphate 0.02-0.1%, remaining is water.Culture After base packing, cultured inclined-plane seed is inoculated in Shake flask medium on shaking table after cooling, temperature 17-19 by 121 DEG C of sterilizings DEG C, revolving speed 100-150rpm is cultivated 12-18 days.

(3) first class seed pot seed culture

First class seed pot culture medium: volume ratio (%) is counted by weight: glucose 1.0-2.0%, corn flour 1.5-2.5%, Molasses 0.5-1.5%, potato 0-2.0%, starch 0-2.0%；Dried silkworm chrysalis meal 1.5-3.5%, soybean cake powder 0-1.5%, yellow bean sprout Powder 0-1.5%, yeast powder 0.2-1.0%, wheat bran 0.5-1.5%, peptone 0-1.0%；Magnesium sulfate 0.01-0.05%, phosphoric acid Potassium dihydrogen 0.02-0.1%, sodium selenite 0-0.001%, remaining is water.After the sterilized cooling of first class seed pot culture medium, it will shake Bottle seed is inoculated in first class seed pot with 5-10% inoculum concentration, controls 17-19 DEG C of temperature, and ventilate 1:0.2-0.4vvm, cultivates 6-8 It.

(4) secondary seed tank seed culture

Secondary seed tank culture medium: volume ratio (%) is counted by weight: glucose 1.0-2.0%, corn flour 1.5-2.5%, Molasses 0.5-1.5%, potato 0-2.0%, starch 0-2.0%；Dried silkworm chrysalis meal 1.5-3.5%, soybean cake powder 0-1.5%, yellow bean sprout Powder 0-1.5%, yeast powder 0.2-1.0%, wheat bran 0.5-1.5%, peptone 0-1.0%；Magnesium sulfate 0.01-0.05%, phosphoric acid Potassium dihydrogen 0.02-0.1%, sodium selenite 0-0.002%, remaining is water.After the sterilized cooling of secondary seed tank culture medium, by one Grade seeding tank seed is inoculated in secondary seed tank with 10-20% inoculum concentration, controls 17-19 DEG C of temperature, and ventilate 1:0.2-0.4vvm, Culture 6-8 days.

(5) fermentation tank culture

Fermentation tank culture medium: volume ratio (%) is counted by weight: glucose 1.0-2.0%, corn flour 1.5-2.5%, molasses 0.5-1.5%, potato 0-2.0%, starch 0-2.0%；Dried silkworm chrysalis meal 1.5-3.5%, soybean cake powder 0-1.5%, soybean powder 0- 1.5%, yeast powder 0-1.0%, Se-enriched yeast powder 0-2.5%, wheat bran 0.5-1.5%, peptone 0-1.0%；Magnesium sulfate 0.01- 0.05%, potassium dihydrogen phosphate 0.02-0.1%, sodium selenite 0-0.01%, remaining is water.Fermentation tank culture medium is by disinfection, drop Wen Hou accesses secondary seed tank seed with 10-30% inoculum concentration, controls 17-19 DEG C of temperature, and ventilate 1:0.2-0.4vvm, culture 8-10 days；

(6) it is separated by solid-liquid separation, is dry, detecting and crushing

Tank fermentation liquid will be put to be separated by solid-liquid separation, solid will be dried, component content in the dry mycelium of detection gained: total selenium 25-1350ug/g (detection method: GB 5009.93), organic selenium account for total selenium mass percent w/% >=92% (detection method: In GB1903.22-2016 appendix A A.2), there are also the cordycepic acid of 4-8% (detection methods: Bailing capsule standards of pharmacopoeia version in 2015 1.6-1.6.1), the adenosine (detection method: hundred of 5-8% Cordyceps sinensis polysaccharide (detection method: phend-sulphuric acid) and 0.03-0.17% Enable capsule standards of pharmacopoeia version 1.6-1.6.2 in 2015)；The mycelium powder is broken into 80-100 mesh, mycelium powder finished product is made, at Product physical behavior: it is powdered to yellowish-brown in lark, there is micro- fishy smell, free from extraneous odour takes this product a small amount of, adds methylene blue staining Afterwards, set that oil mirror microscopy under microscope is visible to be had every multicore mycelia.

The present invention has the positive effect that:

1. trace elements of selenium and aweto are organically combined, the selenium-rich for having both cordyceps sinensis and selenium good characteristic is obtained Cordyceps sinensis mycelium powder, this product not only containing the physiological activator of cordyceps sinensis, also containing 0.1% or more it is organic Selenium.It was demonstrated that compared with common aweto product, health care or therapeutic effect are greater than aweto mycelium for numerous researchs Powder single-item and organic selenium single-item.Can be one while playing the physiological action that cordyceps sinensis and selenium are intrinsic and cooperate with.No matter Nutritional supplement as drug, health food and selenium all has practicability.

2. the present invention in selenium-rich aweto mycelium incubation, using seeding tank plus sodium selenite domestication culture by Step adapts to technique, and the sodium selenite amount in culture medium that overcomes increases the inhibition grown to aweto, realizes mycelia for the first time Body yield close to mycelium in the case where 3% (embodiment 12) containing total selenium amount up to 1350 μ g/g, realize the fermentation selenium-rich winter for the first time Worm summer grass bacterium is more than 85% to the utilization rate of inorganic selenium, and organic degree is more than 92%.The defect of the prior art is overcome, is suitble to Industrialization production.

Specific embodiment

Embodiment 1

The preparation of inclined-plane seed: DCH slant medium: sucrose 5.0%, yeast powder 0.3%, tryptone 1.0%, agar Powder 1.0%, with the inorganic ionic solution of pH 5.6-6.0 (0.5 gram of magnesium sulfate, 1 gram of potassium dihydrogen phosphate, 1 gram of sodium chloride, calcium chloride 0.3 gram, 0.01 gram of manganese sulfate, 0.01 gram of zinc sulfate, 0.01 gram of ferrous sulfate, 0.01 gram of copper sulphate, 1000 milli of distilled water constant volume Rise) it prepares.Culture medium is prepared, is dispensed, after disinfection, and incline inclined-plane processed.The aweto of the CICC number 14088 of activation will be passed through Kind is inoculated in slant medium, cultivates 40 days at 15-16 DEG C.

The preparation of shake-flask seed: Shake flask medium: glucose 1.5%, corn flour 2.0%, molasses 0.5%, potato 6.0%；Dried silkworm chrysalis meal 2.5%, soybean powder 1.0%, yeast powder 0.5%, wheat bran 1.0%；Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.02%, remaining is water.Potato is cut into fritter, dried silkworm chrysalis meal, corn flour, wheat bran and the slightly boiled extraction 30min of suitable quantity of water, filtering first After take filtrate, other ingredients are added, sterilize after packing, slant strains are connected to Shake flask medium on shaking table after cooling, temperature 17-19 DEG C, 100-130rpm is cultivated 17 days.

The preparation of seeding tank seed: culture medium is glucose 1.5%, molasses 1.0%, starch 1.5%, dried silkworm chrysalis meal 3.0%, Huang Bean sprouts powder 0.5%, yeast powder 0.5%, peptone 0.5%, wheat bran 1.5%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, Remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction 30min of suitable quantity of water, filtrate is taken after filtering, It is added other ingredients, after sterilizing cooling, shake-flask seed is inoculated with 10% inoculum concentration, 17-19 DEG C of temperature, speed of agitator 100- 120rpm, ventilate 1:0.2-0.4vvm, cultivates 8 days.

Fermentation tank culture: culture medium is glucose 1.5%, molasses 1.0%, starch 1.5%, dried silkworm chrysalis meal 3.0%, soyabean cake Powder 0.5%, yeast powder 0.5%, peptone 0.5%, wheat bran 1.5%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, sub- selenium Sour sodium 0.0002%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean cake powder and the slightly boiled extraction 30min of suitable quantity of water, Filtrate is taken after filtering, other ingredients are added, after sterilizing cooling, with 10% inoculum concentration access seeding tank seed in fermentor, temperature 17-19 DEG C, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, and it is thick to cultivate growth in 7 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, solid is dried, and dry mycelial yield is 34.2 kg/ton Fermentation liquid.Total Se content is 25ug/g, organic selenium 24ug/g (inorganic se use efficiency 93.9%, organic rate in dry mycelium 96%), cordycepic acid 71.60mg/g, Cordyceps sinensis polysaccharide 72.83mg/g, adenosine 0.41mg/g.The dry mycelium be crushed into 80-100 Cordyceps sinensis mycelium powder is made in mesh, and mycelium powder is powdered to yellowish-brown in lark, has micro- fishy smell, and free from extraneous odour takes On a small quantity, after adding methylene blue staining, set that oil mirror microscopy under microscope is visible to be had every multicore mycelia.

Embodiment 2

The preparation of inclined-plane seed: DCH slant medium: sucrose 5.0%, yeast powder 0.3%, tryptone 1.0%, agar Powder 1.0%, with the inorganic ionic solution of pH 5.6-6.0 (0.5 gram of magnesium sulfate, 1 gram of potassium dihydrogen phosphate, 1 gram of sodium chloride, calcium chloride 0.3 gram, 0.01 gram of manganese sulfate, 0.01 gram of zinc sulfate, 0.01 gram of ferrous sulfate, 0.01 gram of copper sulphate, 1000 milli of distilled water constant volume Rise) it prepares.Culture medium is prepared, is dispensed, after disinfection, and incline inclined-plane processed, by the aweto of the CICC number 14088 through overactivation Kind is inoculated in slant medium, cultivates 35 days at 17-18 DEG C.

The preparation of shake-flask seed: Shake flask medium: glucose 1.5%, corn flour 2.0%, molasses 1.0%, potato 6.0%；Dried silkworm chrysalis meal 2.5%, soybean powder 1.0%, yeast powder 0.5%, wheat bran 1.0%；Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.02%, remaining is water.Potato is cut into fritter first, adds dried silkworm chrysalis meal, corn flour, wheat bran and the slightly boiled extraction 30min of suitable quantity of water, mistake Filtrate is taken after filter, other ingredients are added, sterilize after packing, and slant strains are inoculated in Shake flask medium on shaking table after cooling, 17-19 DEG C of temperature, 100-130rpm is cultivated 16 days.

Seeding tank seed preparation: culture medium are as follows: glucose 1.5%, molasses 1.0%, corn flour 1.5%, dried silkworm chrysalis meal 3.0%, soybean powder 0.5%, yeast powder 0.5%, peptone 0.3%, wheat bran 2.0%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.03%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction 30min of suitable quantity of water, after filtering Filtrate is taken, other ingredients are added, after sterilizing cooling, shake-flask seed is inoculated with 10% inoculum concentration, 17-19 DEG C of temperature, stirring turns Fast 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 8 days.

Fermentation tank culture: culture medium is glucose 1.5%, molasses 1.0%, corn flour 1.5%, dried silkworm chrysalis meal 3.0%, soya bean Cake powder 0.5%, yeast powder 0.5%, peptone 0.3%, wheat bran 2.0%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.03% are sub- Sodium selenate 0.0005%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean cake powder and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, after sterilizing cooling, with 10% inoculum concentration inoculation seeding tank seed in fermentation Tank, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, and it is thick to cultivate growth in 8 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, solid is dried, and dry mycelial yield is 33.5 kg/ton Fermentation liquid.Total Se content is 63ug/g, organic selenium 60ug/g (inorganic se use efficiency 92.6%, organic rate in dry mycelium 95.2%), cordycepic acid 76.44mg/g, Cordyceps sinensis polysaccharide 73.13mg/g, adenosine 0.36mg/g.The dry mycelium is crushed, 80- is crossed Cordyceps sinensis mycelium powder is made in 100 meshes, and mycelium powder is powdered to yellowish-brown in lark, has micro- fishy smell, is no different Taste, takes a small amount of, after adding methylene blue staining, sets that oil mirror microscopy under microscope is visible to be had every multicore mycelia.

Embodiment 3

The preparation of inclined-plane seed: DCH slant medium: sucrose 5.0%, yeast powder 0.3%, tryptone 1.0%, agar Powder 1.0%, with the inorganic ionic solution of pH 5.6-6.0 (0.5 gram of magnesium sulfate, 1 gram of potassium dihydrogen phosphate, 1 gram of sodium chloride, calcium chloride 0.3 gram, 0.01 gram of manganese sulfate, 0.01 gram of zinc sulfate, 0.01 gram of ferrous sulfate, 0.01 gram of copper sulphate, 1000 milli of distilled water constant volume Rise) it prepares.Culture medium is prepared, is dispensed, after sterilizing, and incline inclined-plane processed, by the aweto of the CICC number 14088 through overactivation Kind is inoculated in slant medium, cultivates 35 days at 17-18 DEG C.

The preparation of shake-flask seed: Shake flask medium: glucose 1.8%, corn flour 2.0%, molasses 0.5%, potato 6.0%；Silkworm chrysalis 6.0% living, soybean powder 1.0%, yeast powder 0.5%, wheat bran 1.0%；Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.04%, remaining is water.Potato is cut into fritter first, silkworm chrysalis living shreds, and adds corn flour, wheat bran and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, sterilize after packing, after cooling by slant strains be inoculated in Shake flask medium in On shaking table, 17-19 DEG C of temperature, 100-130rpm is cultivated 14 days.

The preparation of seeding tank seed: culture medium is glucose 1.5%, molasses 1.0%, starch 1.5%, dried silkworm chrysalis meal 3.5%, Huang Bean sprouts powder 0.5%, yeast powder 0.5%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, Remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction 30min of suitable quantity of water, filtrate is taken after filtering, It is added other ingredients, after sterilizing cooling, shake-flask seed is inoculated with 10% inoculum concentration, 17-19 DEG C of temperature, speed of agitator 100- 120rpm, ventilate 1:0.2-0.4vvm, cultivates 7 days.

Fermentation tank culture: culture medium are as follows: glucose 1.5%, molasses 1.0%, starch 1.5%, dried silkworm chrysalis meal 3.5%, soya bean Bud powder 0.5%, yeast powder 0.5%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02% are sub- Sodium selenate 0.001%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, after disinfection cooling, with 10% inoculum concentration inoculation seeding tank seed in fermentation Tank controls 17-19 DEG C of temperature, speed of agitator 100-120rpm, and ventilate 1:0.2-0.4vvm, and it is thick to cultivate growth in 8 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, solid is dried, and dry mycelial yield is 34.1 kg/ton Fermentation liquid.Total Se content is 125ug/g, organic selenium 117ug/g (inorganic se use efficiency 94.24%, organic rate in dry mycelium 93.6%), cordycepic acid 69.07mg/g, Cordyceps sinensis polysaccharide 78.45mg/g, adenosine 0.50mg/g.The dry mycelium is crushed, 80- is crossed Cordyceps sinensis mycelium powder is made in 100 meshes, and mycelium powder is powdered to yellowish-brown in lark, has micro- fishy smell, is no different Taste, takes a small amount of, after adding methylene blue staining, sets that oil mirror microscopy under microscope is visible to be had every multicore mycelia.

Embodiment 4

The preparation of inclined-plane seed: DCH slant medium: sucrose 5.0%, yeast powder 0.3%, tryptone 1.0%, agar Powder 1.0%, with the inorganic ionic solution of pH 5.6-6.0 (0.5 gram of magnesium sulfate, 1 gram of potassium dihydrogen phosphate, 1 gram of sodium chloride, calcium chloride 0.3 gram, 0.01 gram of manganese sulfate, 0.01 gram of zinc sulfate, 0.01 gram of ferrous sulfate, 0.01 gram of copper sulphate, 1000 milli of distilled water constant volume Rise) it prepares.It will be inoculated in slant medium by the aweto strain of the CICC number 14088 activated, is cultivated at 15-18 DEG C 36 days.

The preparation of shake-flask seed: Shake flask medium: glucose 1.8%, corn flour 2.0%, molasses 0.5%, potato 6.0%；Dried silkworm chrysalis meal 2.5%, soybean powder 1.0%, yeast powder 0.5%, wheat bran 1.0%；Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.04%, remaining is water.Potato is cut into fritter first, adds dried silkworm chrysalis meal, corn flour, wheat bran and the slightly boiled extraction 30min of suitable quantity of water, mistake Filtrate is taken after filter, other ingredients are added, sterilize after packing, and slant strains are inoculated in Shake flask medium on shaking table after cooling, 17-19 DEG C of temperature, 100-130rpm is cultivated 16.5 days.

The preparation of seeding tank seed: culture medium is glucose 1.5%, molasses 1.0%, starch 1.5%, dried silkworm chrysalis meal 3.5%, Huang Bean sprouts powder 0.5%, yeast powder 0.5%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, Sodium selenite 0.001%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, and after sterilizing cooling, is inoculated with shake-flask seed, temperature 17- with 10% inoculum concentration 19 DEG C, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 7 days.

Fermentation tank culture: culture medium is glucose 1.5%, molasses 1.0%, starch 1.5%, dried silkworm chrysalis meal 3.5%, soyabean cake Powder 0.5%, yeast powder 0.5%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, sub- selenium Sour sodium 0.003%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean cake powder and the slightly boiled extraction 30min of suitable quantity of water, Filtrate is taken after filtering, is added other ingredients, after sterilizing cooling, with 20% inoculum concentration inoculation secondary seed tank seed in fermentor, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, and it is thick to cultivate growth in 8 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, and by solid drying, crushes into Chinese caterpillar fungus bacterial filament powder.It is dry Mycelial yield is 33.6 kg/ton of fermentation liquids.The dry total Se content of mycelium is 365ug/g, organic selenium 340ug/g, cordycepic acid 63.77mg/g, Cordyceps sinensis polysaccharide 75.43mg/g, adenosine 0.32mg/g.Inorganic se use efficiency 89.7%, organic rate 93.2%.It will The dry mycelium crushed 80-100 mesh and cordyceps sinensis mycelium powder be made.Mycelium powder is in lark to yellowish-brown powder Shape, has micro- fishy smell, free from extraneous odour, takes a small amount of, after adding methylene blue staining, sets that oil mirror microscopy under microscope is visible to be had every multicore bacterium Silk.

Embodiment 5

The preparation of inclined-plane seed: DCH slant medium: sucrose 5.0%, yeast powder 0.3%, tryptone 1.0%, agar Powder 1.0%, with the inorganic ionic solution of pH 5.6-6.0 (0.5 gram of magnesium sulfate, 1 gram of potassium dihydrogen phosphate, 1 gram of sodium chloride, calcium chloride 0.3 gram, 0.01 gram of manganese sulfate, 0.01 gram of zinc sulfate, 0.01 gram of ferrous sulfate, 0.01 gram of copper sulphate, 1000 milli of distilled water constant volume Rise) it prepares.After sterilizing cooling, it will be inoculated in slant medium by the aweto strain of the CICC number 14088 activated, 15-18 DEG C is cultivated 35 days.

The preparation of shake-flask seed: Shake flask medium: glucose 1.0%, corn flour 2.5%, molasses 1.0%, potato 2.0%；Silkworm chrysalis 6.0% living, soybean powder 0.5%, yeast powder 0.2%, wheat bran 1.0%；Magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.1%, remaining is water.Potato is cut into fritter first, silkworm chrysalis living shreds, and adds corn flour, wheat bran and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, sterilize after packing, after cooling by slant strains be inoculated in Shake flask medium in On shaking table, 17-19 DEG C of temperature, 100-130rpm is cultivated 12 days.

First class seed pot seed preparation: culture medium are as follows: glucose 1.0%, corn flour 2.5%, molasses 1.5%；Dried silkworm chrysalis meal 1.5%, soybean cake powder 1.5%, yeast powder 0.5%, wheat bran 0.5%, peptone 1.0%；Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.05%；Remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean cake powder and the slightly boiled extraction 30min of suitable quantity of water, after filtering Filtrate is taken, is added other ingredients, after sterilizing cooling, shake-flask seed is accessed with 5% inoculum concentration, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 8 days.

Secondary seed tank seed culture: culture medium is glucose 1.0%, corn flour 2.5%, molasses 1.5%；Dried silkworm chrysalis meal 1.5%, soybean cake powder 1.5%, yeast powder 0.5%, wheat bran 0.5%, peptone 1.0%；Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.05%, sodium selenite 0.001%；Remaining is water.It is first that dried silkworm chrysalis meal, corn flour, wheat bran, soybean cake powder and suitable quantity of water is slightly boiled 30min is extracted, takes filtrate after filtering, other ingredients are added, after sterilizing cooling, first class seed pot kind is accessed with 10% inoculum concentration Son, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 8 days.

Fermentation tank culture: culture medium are as follows: glucose 1.0%, corn flour 2.5%, molasses 1.5%；Dried silkworm chrysalis meal 1.5%, it is yellow Beancake powder 1.5%, yeast powder 0.5%, wheat bran 0.5%, peptone 1.0%；Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.05%, Sodium selenite 0.0045%；Remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean cake powder and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, and after sterilizing cooling, is inoculated with secondary seed tank seed with 20% inoculum concentration, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 9 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, and by solid drying, crushes into selenium-rich Chinese caterpillar fungus bacterium powder. Mycelial yield is 28.6 kg/ton of fermentation liquids.Bacterium powder Se content is 625ug/g, organic selenium 585ug/g, cordycepic acid 83.66mg/g, Cordyceps sinensis polysaccharide 83.66mg/g, adenosine 0.30mg/g.The utilization rate 87.2% of selenium, organic rate 93.6%.It should Dry mycelium crushed 80-100 mesh and cordyceps sinensis mycelium powder be made.Mycelium powder is powdered to yellowish-brown in lark, With micro- fishy smell, free from extraneous odour takes a small amount of, after adding methylene blue staining, sets that oil mirror microscopy under microscope is visible to be had every multicore mycelia.

Embodiment 6

The preparation of inclined-plane seed: DCH slant medium: sucrose 5.0%, yeast powder 0.3%, tryptone 1.0%, agar Powder 1.0%, with the inorganic ionic solution of pH 5.6-6.0 (0.5 gram of magnesium sulfate, 1 gram of potassium dihydrogen phosphate, 1 gram of sodium chloride, calcium chloride 0.3 gram, 0.01 gram of manganese sulfate, 0.01 gram of zinc sulfate, 0.01 gram of ferrous sulfate, 0.01 gram of copper sulphate, 1000 milli of distilled water constant volume Rise) it prepares.It will be inoculated in slant medium by the aweto strain of the CICC number 14088 activated, is cultivated at 15-18 DEG C 40 days.

The preparation of shake-flask seed: Shake flask medium: glucose 1.5%, corn flour 1.5%, molasses 1.5%, potato 3.0%；Silkworm chrysalis 4.0% living, soybean powder 1.0%, yeast powder 1.0%, wheat bran 1.5%；Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.05%, remaining is water.Potato is cut into fritter first, silkworm chrysalis living shreds, and adds corn flour, wheat bran and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, sterilize after packing, after cooling by slant strains be inoculated in Shake flask medium in On shaking table, 17-19 DEG C of temperature, 100-130rpm is cultivated 15 days.

The preparation of first class seed pot seed: culture medium is glucose 1.5%, corn flour 2.0%, molasses 1.5%；Dried silkworm chrysalis meal 2.5%, soybean powder 0.5%, yeast powder 1.0%, wheat bran 1.0%, peptone 1.0%；Magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, sodium selenite 0.001%；Remaining is water.It is first that dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and suitable quantity of water is slightly boiled 30min is extracted, takes filtrate after filtering, other ingredients are added, after sterilizing cooling, shake-flask seed, temperature are accessed with 10% inoculum concentration 17-19 DEG C, speed of agitator 100-120rpm of degree, ventilate 1:0.2-0.4vvm, cultivates 7 days.

Secondary seed tank seed culture: culture medium is glucose 1.5%, corn flour 2.0%, molasses 1.5%；Dried silkworm chrysalis meal 2.5%, soybean powder 0.5%, yeast powder 1.0%, wheat bran 1.0%, peptone 1.0%；Magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, sodium selenite 0.002%；Remaining is water.It is first that dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and suitable quantity of water is slightly boiled 30min is extracted, takes filtrate after filtering, other ingredients are added, after sterilizing cooling, first order seed kind is accessed with 15% inoculum concentration Son, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 8 days.

Fermentation tank culture: culture medium is glucose: 1.5%, corn flour 2.0%, and molasses 1.5%；Dried silkworm chrysalis meal 2.5%, it is yellow Bean sprouts powder 0.5%, yeast powder 1.0%, wheat bran 1.0%, peptone 1.0%；Magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1% are sub- Sodium selenate 0.006%；Remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, and after sterilizing cooling, accesses secondary seed tank seed with 20% inoculum concentration. 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, and it is thick to cultivate growth in 8 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, and by solid drying, crushes into Chinese caterpillar fungus bacterium powder.Bacterium powder Yield is 32.1 kg/ton of fermentation liquids.Bacterium powder Se content is 760ug/g, organic selenium 710ug/g, cordycepic acid 71.60mg/g, worm Grass polysaccharide 53.0mg/g, adenosine 1.05mg/g.The utilization rate 89.2% of inorganic selenium, organic rate 93.4%.By the dry mycelium It crushed 80-100 mesh and cordyceps sinensis mycelium powder be made.Mycelium powder is powdered to yellowish-brown in lark, has micro- raw meat Taste, free from extraneous odour takes a small amount of, after adding methylene blue staining, sets that oil mirror microscopy under microscope is visible to be had every multicore mycelia.

Embodiment 7

The preparation of inclined-plane seed: DCH slant medium: sucrose 5.0%, yeast powder 0.3%, tryptone 1.0%, agar Powder 1.0%, with the inorganic ionic solution of pH 5.6-6.0 (0.5 gram of magnesium sulfate, 1 gram of potassium dihydrogen phosphate, 1 gram of sodium chloride, calcium chloride 0.3 gram, 0.01 gram of manganese sulfate, 0.01 gram of zinc sulfate, 0.01 gram of ferrous sulfate, 0.01 gram of copper sulphate, 1000 milli of distilled water constant volume Rise) it prepares.It will be inoculated in slant medium by the aweto strain of the CICC number 14088 activated, is cultivated at 15-18 DEG C 38 days.

The preparation of shake-flask seed: Shake flask medium: glucose 1.5%, corn flour 2.0%, molasses 1.0%, potato 5.0%；Silkworm chrysalis 5.0% living, soybean powder 1.0%, yeast powder 0.4%, wheat bran 1.5%；Magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, remaining is water.Potato is cut into fritter first, silkworm chrysalis living shreds, and adds corn flour, wheat bran and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, sterilize after packing, after cooling by slant strains be inoculated in Shake flask medium in On shaking table, 17-19 DEG C of temperature, 100-130rpm is cultivated 16 days.

The preparation of first class seed pot seed: culture medium is glucose 1.5%, molasses 1.0%, 2.0% dried silkworm chrysalis meal of corn flour 2.5%, soybean powder 1.0%, yeast powder 0.5%, peptone 1.0%, wheat bran 1.0%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, sodium selenite 0.001%, remaining is water.It is first that dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and suitable quantity of water is slightly boiled 30min is extracted, takes filtrate after filtering, other ingredients are added, after sterilizing cooling, with 8% inoculum concentration inoculation shake-flask seed in level-one Seeding tank, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 8 days.

Secondary seed tank seed preparation: culture medium are as follows: glucose 1.5%, molasses 1.0%, corn flour 2.0%, dried silkworm chrysalis meal 2.5%, soybean powder 1.0%, yeast powder 0.5%, peptone 1.0%, wheat bran 1.0%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, sodium selenite 0.002%, remaining is water.It is first that dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and suitable quantity of water is slightly boiled 30min is extracted, takes filtrate after filtering, other ingredients are added, after sterilizing cooling, first class seed pot kind is inoculated with 20% inoculum concentration Son, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 6 days.

Fermentation tank culture: culture medium are as follows: glucose 1.5%, molasses 1.0%, 2.0% dried silkworm chrysalis meal 2.5% of corn flour, soya bean Bud powder 1.0%, yeast powder 0.5%, peptone 1.0%, wheat bran 1.0%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02% are sub- Sodium selenate 0.007%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, and after sterilizing cooling, is inoculated with secondary seed tank seed, temperature with 25% inoculum concentration 17-19 DEG C, speed of agitator 100-120rpm of degree, ventilate 1:0.2-0.4vvm, cultivates 10 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, and by solid drying, crushes into Chinese caterpillar fungus bacterial filament powder.It is dry Mycelial yield is 30.4 kg/ton of fermentation liquids, and the dry total Se content of mycelium is 855ug/g, organic selenium 830ug/g, cordycepic acid 48.79mg/g, Cordyceps sinensis polysaccharide 78.53mg/g, adenosine 0.88mg/g.Inorganic se use efficiency 94.05%, organic rate 97.1%. The dry mycelium be crushed into 80-100 mesh, cordyceps sinensis mycelium powder is made.Mycelium powder is in lark to yellowish-brown powder Shape, has micro- fishy smell, free from extraneous odour, takes a small amount of, after adding methylene blue staining, sets that oil mirror microscopy under microscope is visible to be had every multicore bacterium Silk.

Embodiment 8

The preparation of inclined-plane seed: DCH slant medium: sucrose 5.0%, yeast powder 0.3%, tryptone 1.0%, agar Powder 1.0%, with the inorganic ionic solution of pH 5.6-6.0 (0.5 gram of magnesium sulfate, 1 gram of potassium dihydrogen phosphate, 1 gram of sodium chloride, calcium chloride 0.3 gram, 0.01 gram of manganese sulfate, 0.01 gram of zinc sulfate, 0.01 gram of ferrous sulfate, 0.01 gram of copper sulphate, 1000 milli of distilled water constant volume Rise) it prepares.It will be inoculated in slant medium by the aweto strain of the CICC number 14088 activated, is cultivated at 15-18 DEG C 37 days.

The preparation of shake-flask seed: Shake flask medium: glucose 2.0%, corn flour 2.0%, molasses 0.5%, potato 6.0%；Dried silkworm chrysalis meal 2.5%, soybean powder 1.0%, yeast powder 0.5%, wheat bran 1.0%；Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.04%, remaining is water.Potato is cut into fritter, dried silkworm chrysalis meal, corn flour, wheat bran and the slightly boiled extraction 30min of suitable quantity of water, filtering first After take filtrate, other ingredients are added, sterilize after packing, slant strains are inoculated in Shake flask medium on shaking table after cooling, temperature 17-19 DEG C of degree, 100-130rpm are cultivated 18 days.

First class seed pot seed preparation: culture medium are as follows: glucose 1.5%, molasses 1.0%, starch 1.5%, dried silkworm chrysalis meal 3.5%, soybean powder 0.5%, yeast powder 0.5%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, sodium selenite 0.001%, remaining is water.It is first that dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and suitable quantity of water is slightly boiled 30min is extracted, takes filtrate after filtering, other ingredients are added, after sterilizing cooling, with 10% inoculum concentration inoculation shake-flask seed in one Grade seeding tank, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 6 days.

Secondary seed tank seed preparation: culture medium are as follows: glucose 1.5%, molasses 1.0%, starch 1.5%, dried silkworm chrysalis meal 3.5%, soybean powder 0.5%, yeast powder 0.5%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, sodium selenite 0.002%, remaining is water.It is first that dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and suitable quantity of water is slightly boiled 30min is extracted, takes filtrate after filtering, other ingredients are added, after sterilizing cooling, with 20% inoculum concentration inoculation first order seed in second level Seeding tank, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 8 days.

Ferment tank culture: culture medium is glucose: 1.5%, molasses 1.0%, and starch 1.5%, dried silkworm chrysalis meal 3.5%, Soybean powder 0.5%, yeast powder 0.5%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, sodium selenite 0.008%, remaining is water.It is first that dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and suitable quantity of water is slightly boiled 30min is extracted, takes filtrate after filtering, other ingredients are added, after sterilizing cooling, secondary seed tank seed is inoculated with 30% inoculum concentration In fermentor, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, and it is thick to cultivate growth in 9.5 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, and by solid drying, crushes into cordyceps sinensis mycelium powder. Dry mycelial yield is 29.8 kg/ton of fermentation liquids, and the dry total Se content of mycelium is 1120ug/g, organic selenium 1031ug/g, worm Oxalic acid 50.00mg/g, Cordyceps sinensis polysaccharide 80.20mg/g, adenosine 0.89mg/g.Inorganic se use efficiency 91.5%, organic rate 92.1%.The dry mycelium be crushed into 80-100 mesh, cordyceps sinensis mycelium powder is made.Mycelium powder is in lark to Huang Brown powder shape, has micro- fishy smell, free from extraneous odour, take it is a small amount of, after adding methylene blue staining, set oil mirror microscopy under microscope it is visible have every Multicore mycelia.

Embodiment 9

The preparation of first class seed pot seed: culture medium is glucose 1.5%, molasses 1.0%, 2.0% dried silkworm chrysalis meal of corn flour 2.5%, soybean powder 1.0%, yeast powder 0.5%, peptone 1.0%, wheat bran 1.0%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction 30min of suitable quantity of water, after filtering Filtrate is taken, other ingredients are added, after sterilizing cooling, with 8% inoculum concentration inoculation shake-flask seed in first class seed pot, temperature 17-19 DEG C, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 8 days.

Secondary seed tank seed preparation: culture medium are as follows: glucose 1.5%, molasses 1.0%, corn flour 2.0%, dried silkworm chrysalis meal 2.5%, soybean powder 1.0%, yeast powder 0.5%, peptone 1.0%, wheat bran 1.0%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, sodium selenite 0.002%, remaining is water.It is first that dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and suitable quantity of water is slightly boiled 30min is extracted, takes filtrate after filtering, other ingredients are added, after sterilizing cooling, first class seed pot kind is inoculated with 20% inoculum concentration Son, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 8 days.

Fermentation tank culture: culture medium are as follows: glucose 1.5%, molasses 1.0%, 2.0% dried silkworm chrysalis meal 2.5% of corn flour, soya bean Bud powder 1.0%, yeast powder 0.5%, peptone 1.0%, wheat bran 1.0%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02% are sub- Sodium selenate 0.009%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, and after sterilizing cooling, is inoculated with secondary seed tank seed, temperature with 25% inoculum concentration 17-19 DEG C, speed of agitator 100-120rpm of degree, ventilate 1:0.2-0.4vvm, cultivates 9.5 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, and by solid drying, crushes into Chinese caterpillar fungus bacterial filament powder.It is dry Mycelial yield is 28.2 kg/ton of fermentation liquids, and the dry total Se content of mycelium is 1250ug/g, organic selenium 1156ug/g, cordyceps sinensis Sour 60.79mg/g, Cordyceps sinensis polysaccharide 76.05mg/g, adenosine 0.68mg/g.Inorganic se use efficiency 85.9%, organic rate 92.48%.The dry mycelium be crushed into 80-100 mesh, cordyceps sinensis mycelium powder is made.Mycelium powder is in lark to Huang Brown powder shape, has micro- fishy smell, free from extraneous odour, take it is a small amount of, after adding methylene blue staining, set oil mirror microscopy under microscope it is visible have every Multicore mycelia.

Embodiment 10

The preparation of shake-flask seed: Shake flask medium: glucose 2.0%, corn flour 2.0%, molasses 0.5%, potato 6.0%；Dried silkworm chrysalis meal 2.5%, soybean powder 1.0%, yeast powder 0.5%, wheat bran 1.0%；Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.04%, remaining is water.Potato is cut into fritter first, adds dried silkworm chrysalis meal, corn flour, wheat bran and the slightly boiled extraction 30min of suitable quantity of water, mistake Filtrate is taken after filter, other ingredients are added, sterilize after packing, and slant strains are inoculated in Shake flask medium on shaking table after cooling, 17-19 DEG C of temperature, 100-130rpm is cultivated 16 days.

First class seed pot seed preparation: culture medium are as follows: glucose 1.5%, molasses 1.0%, starch 1.5%, dried silkworm chrysalis meal 3.5%, soybean powder 0.5%, yeast powder 0.5%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, sodium selenite 0.001%, remaining is water.It is first that dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and suitable quantity of water is slightly boiled 30min is extracted, takes filtrate after filtering, other ingredients are added, after sterilizing cooling, with 10% inoculum concentration inoculation shake-flask seed in one Grade seeding tank, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 7 days.

Ferment tank culture: culture medium is glucose: 1.5%, molasses 1.0%, and starch 1.5%, dried silkworm chrysalis meal 3.5%, Soybean powder 0.5%, yeast powder 0.5%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, sodium selenite 0.01%, remaining is water.It is first that dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and suitable quantity of water is slightly boiled 30min is extracted, takes filtrate after filtering, other ingredients are added, after sterilizing cooling, secondary seed tank seed is inoculated with 30% inoculum concentration In fermentor, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, and it is thick to cultivate growth in 10 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, and by solid drying, crushes into Chinese caterpillar fungus bacterial filament powder.It is dry Mycelial yield is 28.7 kg/ton of fermentation liquids, and the dry total Se content of mycelium is 1350ug/g, organic selenium 1251ug/g, cordyceps sinensis Sour 61.00mg/g, Cordyceps sinensis polysaccharide 70.20mg/g, adenosine 0.78mg/g.Inorganic se use efficiency 85.2%, organic rate 92.7%. The dry mycelium be crushed into 80-100 mesh, cordyceps sinensis mycelium powder is made.Mycelium powder is in lark to yellowish-brown powder Shape, has micro- fishy smell, free from extraneous odour, takes a small amount of, after adding methylene blue staining, sets that oil mirror microscopy under microscope is visible to be had every multicore bacterium Silk.

Embodiment 11

The preparation of inclined-plane seed: DCH slant medium: sucrose 5.0%, yeast powder 0.3%, tryptone 1.0%, agar Powder 1.0%, with the inorganic ionic solution of pH 5.6-6.0 (0.5 gram of magnesium sulfate, 1 gram of potassium dihydrogen phosphate, 1 gram of sodium chloride, calcium chloride 0.3 gram, 0.01 gram of manganese sulfate, 0.01 gram of zinc sulfate, 0.01 gram of ferrous sulfate, 0.01 gram of copper sulphate, 1000 milli of distilled water constant volume Rise) it prepares.Culture medium is prepared, is dispensed, after disinfection, and incline inclined-plane processed, will pass through the aweto of the CICC number 14088 of activation Kind is inoculated in slant medium, cultivates 35 days at 17-18 DEG C.

The preparation of shake-flask seed: Shake flask medium: glucose 1.6%, corn flour 2.0%, molasses 0.8%, potato 6.0%；Silkworm chrysalis 5.0% living, soybean powder 1.0%, yeast powder 0.5%, wheat bran 1.0%；Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.04%, remaining is water.Potato is cut into fritter first, silkworm chrysalis living shreds, and adds corn flour, wheat bran and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, sterilize after packing, after cooling by slant strains be inoculated in Shake flask medium in On shaking table, 17-19 DEG C of temperature, 100-130rpm is cultivated 17 days.

The preparation of seeding tank seed: culture medium is glucose 1.5%, molasses 1.0%, starch 1.5%, dried silkworm chrysalis meal 3.5%, Huang Bean sprouts powder 0.5%, yeast powder 0.5%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, Remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction 30min of suitable quantity of water, filtrate is taken after filtering, It is added other ingredients, after sterilizing cooling, shake-flask seed is inoculated with 10% inoculum concentration, 17-19 DEG C of temperature, speed of agitator 100- 120rpm, ventilate 1:0.2-0.4vvm, cultivates 8 days.

Fermentation tank culture: culture medium is glucose 1.5%, molasses 1.0%, starch 1.5%, dried silkworm chrysalis meal 3.5%, yellow bean sprout Powder 0.5%, Se-enriched yeast powder (total selenium 1000ug/g) 0.5%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, phosphoric acid Potassium dihydrogen 0.02%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction 30min of suitable quantity of water, Filtrate is taken after filtering, other ingredients are added, after sterilizing cooling, with 20% inoculum concentration access seeding tank seed in fermentor, temperature 17-19 DEG C, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, and it is thick to cultivate growth in 9 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, solid is dried, 40.2 kg/ton of dry mycelial yield hairs Zymotic fluid；The dry total Se content of mycelium is 95ug/g, organic selenium 92ug/g；The dry mycelium includes cordycepic acid 70.67mg/g, cordyceps sinensis Polysaccharide 75.80mg/g, adenosine 0.39mg/g.The dry mycelium be crushed into 80-100 mesh, cordyceps sinensis mycelium powder is made. Mycelium powder is powdered to yellowish-brown in lark, has micro- fishy smell, and free from extraneous odour takes on a small quantity, after adding methylene blue staining, sets micro- Oil mirror microscopy is visible under mirror has every multicore mycelia.

Embodiment 12

The preparation of inclined-plane seed: DCH slant medium: sucrose 5.0%, yeast powder 0.3%, tryptone 1.0%, agar Powder 1.0%, with the inorganic ionic solution of pH 5.6-6.0 (0.5 gram of magnesium sulfate, 1 gram of potassium dihydrogen phosphate, 1 gram of sodium chloride, calcium chloride 0.3 gram, 0.01 gram of manganese sulfate, 0.01 gram of zinc sulfate, 0.01 gram of ferrous sulfate, 0.01 gram of copper sulphate, 1000 milli of distilled water constant volume Rise) it prepares.Culture medium is prepared, is dispensed, after disinfection, and incline inclined-plane processed, will pass through the aweto of the CICC number 14088 of activation Kind is inoculated in slant medium, cultivates 38 days at 17-18 DEG C.

The preparation of shake-flask seed: Shake flask medium: glucose 1.6%, corn flour 2.0%, molasses 1.0%, potato 6.0%；Silkworm chrysalis 6.0% living, soybean powder 1.0%, yeast powder 0.5%, wheat bran 1.0%；Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.04%, remaining is water.Potato is cut into fritter first, silkworm chrysalis living shreds, and adds corn flour, wheat bran and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, sterilize after packing, after cooling by slant strains be inoculated in Shake flask medium in On shaking table, 17-19 DEG C of temperature, 100-130rpm is cultivated 16 days.

Seeding tank seed preparation: culture medium be glucose 1.5%, molasses 1.0%, corn flour 2.0%, dried silkworm chrysalis meal 3.5%, Soybean cake powder 0.5%, yeast powder 0.5%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.02%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction 30min of suitable quantity of water, after filtering Filtrate is taken, other ingredients are added, after sterilizing cooling, shake-flask seed is inoculated with 10% inoculum concentration, 17-19 DEG C of temperature, stirring turns Fast 100-120rpm, ventilate 1:0.2-0.4vvm, cultivates 8 days.

Fermentation tank culture: culture medium is glucose 1.5%, molasses 1.0%, corn flour 2.0%, dried silkworm chrysalis meal 3.0%, soya bean Cake powder 1.0%, Se-enriched yeast powder (total selenium 1000ug/g) 1.0%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, phosphorus Acid dihydride potassium 0.02%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, after sterilizing cooling, with 20% inoculum concentration inoculation seeding tank seed in fermentation Tank, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, and it is thick to cultivate growth in 8 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, solid is dried, 36.1 kg/ton of dry mycelial yield hairs Zymotic fluid.The dry total Se content of mycelium is 245ug/g, organic selenium 236ug/g；The dry mycelium includes cordycepic acid 59.23mg/g, worm The dry mycelium crushed 80-100 mesh and aweto mycelium is made by grass polysaccharide 63.95mg/g, adenosine 1.68mg/g Powder.Mycelium powder is powdered to yellowish-brown in lark, has micro- fishy smell, and free from extraneous odour takes on a small quantity, after adding methylene blue staining, sets Oil mirror microscopy is visible under microscope has every multicore mycelia.

Embodiment 13

Fermentation tank culture: culture medium is glucose 1.5%, molasses 1.0%, corn flour 2.0%, dried silkworm chrysalis meal 3.0%, soya bean Cake powder 1.0%, Se-enriched yeast powder (total selenium 1000ug/g) 2.0%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, phosphorus Acid dihydride potassium 0.02%, sodium selenite 0.003%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and suitable The slightly boiled extraction 30min of water is measured, takes filtrate after filtering, other ingredients are added, after sterilizing cooling, seeding tank is accessed with 15% inoculum concentration Seed is in fermentor, and 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, and it is thick to cultivate growth in 8 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, solid is dried, 36.1 kg/ton of dry mycelial yield hairs Zymotic fluid.The dry total Se content of mycelium is 740ug/g, organic selenium 715ug/g；The dry mycelium includes cordycepic acid 69.30mg/g, worm The dry mycelium crushed 80-100 mesh and aweto mycelium is made by grass polysaccharide 56.92mg/g, adenosine 1.08mg/g Powder.Mycelium powder is powdered to yellowish-brown in lark, has micro- fishy smell, and free from extraneous odour takes on a small quantity, after adding methylene blue staining, sets Oil mirror microscopy is visible under microscope has every multicore mycelia.

Embodiment 14

The preparation of shake-flask seed: Shake flask medium: glucose 1.6%, corn flour 2.0%, molasses 1.0%, potato 6.0%；Silkworm chrysalis 6.0% living, soybean powder 1.0%, yeast powder 0.5%, wheat bran 1.0%；Magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.04%, remaining is water.Potato is cut into fritter first, silkworm chrysalis living shreds, and adds corn flour, wheat bran and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, sterilize after packing, after cooling by slant strains be inoculated in Shake flask medium in On shaking table, 17-19 DEG C of temperature, 100-130rpm is cultivated 17 days.

Fermentation tank culture: culture medium is glucose 1.5%, molasses 1.0%, corn flour 2.0%, dried silkworm chrysalis meal 3.0%, soya bean Cake powder 1.0%, Se-enriched yeast powder (total selenium 1000ug/g) 2.5%, peptone 0.2%, wheat bran 1.5%, magnesium sulfate 0.01%, phosphorus Acid dihydride potassium 0.02%, remaining is water.First by dried silkworm chrysalis meal, corn flour, wheat bran, soybean powder and the slightly boiled extraction of suitable quantity of water 30min takes filtrate after filtering, and other ingredients are added, after sterilizing cooling, with 15% inoculum concentration access seeding tank seed in fermentation Tank, 17-19 DEG C of temperature, speed of agitator 100-120rpm, ventilate 1:0.2-0.4vvm, and it is thick to cultivate growth in 8 days.

Downstream processing: putting tank fermentation liquid and be separated by solid-liquid separation, solid is dried, 38.0 kg/ton of dry mycelial yield hairs Zymotic fluid.The dry total Se content of mycelium is 525ug/g, organic selenium 506ug/g；The dry mycelium includes cordycepic acid 70.35mg/g, worm The dry mycelium crushed 80-100 mesh and aweto mycelium is made by grass polysaccharide 56.09mg/g, adenosine 0.78mg/g Powder.Mycelium powder is powdered to yellowish-brown in lark, has micro- fishy smell, and free from extraneous odour takes on a small quantity, after adding methylene blue staining, sets Oil mirror microscopy is visible under microscope has every multicore mycelia.

Claims

1. a kind of fermented cordyceps sinensis mycelia powder, which is characterized in that the cordyceps sinensis mycelium powder is to be with aweto Strain, by adding sodium selenite or/and Se-enriched yeast liquid Submerged fermentation in the medium, fermentation liquid is by solid-liquid point From and drying process, the dry mycelium of gained include total selenium 25-1350ug/g, wherein organic selenium be not less than 92%, by the mycelium It is ground into 80-100 mesh and mycelium powder is made, mycelium powder has micro- fishy smell in lark to yellowish-brown, and free from extraneous odour takes a small amount of After adding methylene blue staining, set that oil mirror microscopy under microscope is visible to be had every multicore mycelia.

2. a kind of fermented cordyceps sinensis mycelia powder, which is characterized in that the cordyceps sinensis mycelium powder is to be with aweto Strain, by adding sodium selenite or/and Se-enriched yeast liquid Submerged fermentation in the medium, fermentation liquid is by solid-liquid point From and drying process, the dry mycelium of gained include total selenium 500-1350ug/g, wherein organic selenium be not less than 92%, by the mycelium It is ground into 80-100 mesh and mycelium powder is made, mycelium powder has micro- fishy smell in lark to yellowish-brown, and free from extraneous odour takes a small amount of After adding methylene blue staining, set that oil mirror microscopy under microscope is visible to be had every multicore mycelia.

3. a kind of fermented cordyceps sinensis mycelia powder, which is characterized in that the cordyceps sinensis mycelium powder is to be with aweto Strain, by adding sodium selenite or/and Se-enriched yeast liquid Submerged fermentation in the medium, fermentation liquid is by solid-liquid point From and drying process, the dry mycelium of gained include total selenium 1000-1350ug/g, wherein organic selenium be not less than 92%, by the mycelia Body is ground into 80-100 mesh and mycelium powder is made, and mycelium powder has micro- fishy smell in lark to yellowish-brown, and free from extraneous odour takes few After amount plus methylene blue staining, set that oil mirror microscopy under microscope is visible to be had every multicore mycelia.

4. a kind of fermented cordyceps sinensis mycelia powder, which is characterized in that the cordyceps sinensis mycelium powder is to be with aweto Strain, by adding sodium selenite or/and Se-enriched yeast liquid Submerged fermentation in the medium, fermentation liquid is by solid-liquid point From and drying process, the dry mycelium of gained include total selenium 25-1350ug/g, wherein organic selenium is not less than 92%, in dry mycelium Also containing the cordycepic acid of 7-8%, which is broken into 80-100 mesh by the adenosine of 5-8% Cordyceps sinensis polysaccharide and 0.03-0.17% Mycelium powder is made, mycelium powder has micro- fishy smell in lark to yellowish-brown, and free from extraneous odour takes on a small quantity, adds methylene blue staining Afterwards, set that oil mirror microscopy under microscope is visible to be had every multicore mycelia.

5. a kind of fermented cordyceps sinensis mycelia powder, which is characterized in that the cordyceps sinensis mycelium powder is to be with aweto Strain, by adding sodium selenite or/and Se-enriched yeast liquid Submerged fermentation in the medium, fermentation liquid is by solid-liquid point From and drying process, the dry mycelium of gained include total selenium 500-1350ug/g, wherein organic selenium is not less than 92%, in dry mycelium Also containing the cordycepic acid of 7-8%, which is broken into 80-100 mesh by the adenosine of 5-8% Cordyceps sinensis polysaccharide and 0.03-0.17% Mycelium powder is made, mycelium powder has micro- fishy smell in lark to yellowish-brown, and free from extraneous odour takes this product a small amount of, methylenum careuleum is added to contaminate After color, set that oil mirror microscopy under microscope is visible to be had every multicore mycelia.

6. a kind of fermented cordyceps sinensis mycelia powder, which is characterized in that the cordyceps sinensis mycelium powder is to be with aweto Strain, by adding sodium selenite or/and Se-enriched yeast liquid Submerged fermentation in the medium, fermentation liquid is by solid-liquid point From and drying process, the dry mycelium of gained include total selenium 1000-1350ug/g, wherein organic selenium be not less than 92%, dry mycelium Inside also containing the cordycepic acid of 7-8%, which is broken into 80-100 by the adenosine of 5-8% Cordyceps sinensis polysaccharide and 0.03-0.17% Mycelium powder is made in mesh, and mycelium powder has micro- fishy smell in lark to yellowish-brown, and free from extraneous odour takes this product a small amount of, adds methylenum careuleum After dyeing, set that oil mirror microscopy under microscope is visible to be had every multicore mycelia.

7. the method for producing claim 1-6 fermented cordyceps sinensis mycelia powder characterized by comprising slant strains culture, Shake-flask seed culture, first class seed pot culture, secondary seed tank culture, fermentation tank culture are separated by solid-liquid separation, dry, detection and powder Broken technical process, the specific steps are as follows:

(1) slant strains culture

Slant strains culture selects Chinese industrial Microbiological Culture Collection administrative center DCH culture medium, by weight volume ratio (%) Meter: sucrose 5.0%, yeast powder 0.3%, tryptone 1.0%, agar powder 1.0%, inorganic ionic solution pH 5.6-6.0 (sulphur 0.5 gram of sour magnesium, 1 gram of potassium dihydrogen phosphate, 1 gram of sodium chloride, 0.3 gram of calcium chloride, 0.01 gram of manganese sulfate, 0.01 gram of zinc sulfate, sulfuric acid Ferrous 0.01 gram, 0.01 gram of copper sulphate, 1000 milliliters of distilled water constant volume).121 DEG C of sterilizings after packing, incline inclined-plane processed, will be through making a living The strain of change is inoculated in slant medium, 15-18 DEG C culture 30-40 days；

(2) shake-flask seed culture

Shake flask medium: volume ratio (%) is counted by weight: glucose 1.0-2.0%, corn flour 1.0-2.5%, molasses 0.5- 1.5%, potato 1.0-6.0%；Dried silkworm chrysalis meal 0-3.0%, silkworm chrysalis 0-6.0%, soybean powder 0-1.5%, yeast powder 0.2- living 1.0%, wheat bran 0.5-1.5%；Magnesium sulfate 0.01-0.05%, potassium dihydrogen phosphate 0.02-0.1%, remaining is water；Culture medium point After dress, cultured inclined-plane seed is inoculated in Shake flask medium on shaking table after cooling by 121 DEG C of sterilizings, and 17-19 DEG C of temperature, Revolving speed 100-150rpm is cultivated 12-18 days；

(3) first class seed pot seed culture

First class seed pot culture medium: volume ratio (%) is counted by weight: glucose 1.0-2.0%, corn flour 1.5-2.5%, molasses 0.5-1.5%, potato 0-2.0%, starch 0-2.0%；Dried silkworm chrysalis meal 1.5-3.5%, soybean cake powder 0-1.5%, soybean powder 0- 1.5%, yeast powder 0.2-1.0%, wheat bran 0.5-1.5%, peptone 0-1.0%；Magnesium sulfate 0.01-0.05%, biphosphate Potassium 0.02-0.1%, sodium selenite 0-0.001%, remaining is water；After the sterilized cooling of first class seed pot culture medium, by shaking flask kind Son is inoculated in first class seed pot with 5-10% inoculum concentration, controls 17-19 DEG C of temperature, and ventilate 1:0.2-0.4vvm, cultivates 6-8 days；

(4) secondary seed tank seed culture

Secondary seed tank culture medium: volume ratio (%) is counted by weight: glucose 1.0-2.0%, corn flour 1.5-2.5%, molasses 0.5-1.5%, potato 0-2.0%, starch 0-2.0%；Dried silkworm chrysalis meal 1.5-3.5%, soybean cake powder 0-1.5%, soybean powder 0- 1.5%, yeast powder 0.2-1.0%, wheat bran 0.5-1.5%, peptone 0-1.0%；Magnesium sulfate 0.01-0.05%, biphosphate Potassium 0.02-0.1%, sodium selenite 0-0.002%, remaining is water；After the sterilized cooling of secondary seed tank culture medium, by level-one kind Sub- tank seed is inoculated in secondary seed tank with 10-20% inoculum concentration, controls 17-19 DEG C of temperature, and ventilate 1:0.2-0.4vvm, culture 6-8 days；

(5) fermentation tank culture

Fermentation tank culture medium: volume ratio (%) is counted by weight: glucose 1.0-2.0%, corn flour 1.5-2.5%, molasses 0.5- 1.5%, potato 0-2.0%, starch 0-2.0%；Dried silkworm chrysalis meal 1.5-3.5%, soybean cake powder 0-1.5%, soybean powder 0- 1.5%, yeast powder 0-1.0%, Se-enriched yeast powder 0-2.5%, wheat bran 0.5-1.5%, peptone 0-1.0%；Magnesium sulfate 0.01- 0.05%, potassium dihydrogen phosphate 0.02-0.1%, sodium selenite 0-0.01%, remaining is water；Fermentation tank culture medium is by disinfection, drop Wen Hou accesses secondary seed tank seed with 10-30% inoculum concentration, controls 17-19 DEG C of temperature, and ventilate 1:0.2-0.4vvm, culture 8-10 days；

(6) it is separated by solid-liquid separation, is dry, detecting and crushing

Tank fermentation liquid will be put to be separated by solid-liquid separation, solid will be dried, the dry mycelium of detection gained includes total selenium 25-1350ug/ G, organic selenium account for total selenium mass percent w/% >=92%, also containing the cordycepic acid of 4-8%, 5-8% Cordyceps sinensis polysaccharide and 0.03- The mycelium powder is broken into 80-100 mesh and mycelium powder is made by 0.17% adenosine, and mycelium powder is in lark to yellowish-brown toner Last shape, have micro- fishy smell, free from extraneous odour takes this product a small amount of, after adding methylene blue staining, sets oil mirror microscopy under microscope, it is seen that have every Multicore mycelia.