CN106258999B - A kind of thickness pleat Aode mushroom strain, cultural method and application thereof - Google Patents
A kind of thickness pleat Aode mushroom strain, cultural method and application thereof Download PDFInfo
- Publication number
- CN106258999B CN106258999B CN201610658412.3A CN201610658412A CN106258999B CN 106258999 B CN106258999 B CN 106258999B CN 201610658412 A CN201610658412 A CN 201610658412A CN 106258999 B CN106258999 B CN 106258999B
- Authority
- CN
- China
- Prior art keywords
- pleat
- thick
- aode mushroom
- aode
- mushroom
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 126
- 238000000034 method Methods 0.000 title abstract description 18
- 230000003064 anti-oxidating effect Effects 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 51
- 239000000284 extract Substances 0.000 claims description 46
- 239000012530 fluid Substances 0.000 claims description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 11
- 230000003078 antioxidant effect Effects 0.000 claims description 11
- 239000003963 antioxidant agent Substances 0.000 claims description 9
- 239000003814 drug Substances 0.000 claims description 8
- 235000013305 food Nutrition 0.000 claims description 6
- 150000002148 esters Chemical class 0.000 claims description 2
- 150000002576 ketones Chemical class 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 abstract description 52
- 241000233866 Fungi Species 0.000 abstract description 11
- 238000012360 testing method Methods 0.000 abstract description 6
- 241000993288 Oudemansiella Species 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 4
- 239000000796 flavoring agent Substances 0.000 abstract description 3
- 235000019634 flavors Nutrition 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000012364 cultivation method Methods 0.000 abstract description 2
- 239000000470 constituent Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 description 81
- 238000004519 manufacturing process Methods 0.000 description 36
- 235000015099 wheat brans Nutrition 0.000 description 27
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 26
- 240000008397 Ganoderma lucidum Species 0.000 description 22
- 235000001637 Ganoderma lucidum Nutrition 0.000 description 22
- -1 and after testing Substances 0.000 description 21
- 239000001963 growth medium Substances 0.000 description 20
- 239000001965 potato dextrose agar Substances 0.000 description 18
- 239000000469 ethanolic extract Substances 0.000 description 16
- 239000004743 Polypropylene Substances 0.000 description 15
- 229920001155 polypropylene Polymers 0.000 description 15
- 229920000742 Cotton Polymers 0.000 description 13
- 229910000019 calcium carbonate Inorganic materials 0.000 description 13
- 239000007788 liquid Substances 0.000 description 13
- 150000003254 radicals Chemical class 0.000 description 13
- 241000894007 species Species 0.000 description 13
- 230000000694 effects Effects 0.000 description 12
- 238000002360 preparation method Methods 0.000 description 12
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 11
- 238000003306 harvesting Methods 0.000 description 11
- 239000002609 medium Substances 0.000 description 11
- 229920001817 Agar Polymers 0.000 description 10
- 241000894006 Bacteria Species 0.000 description 10
- 239000008272 agar Substances 0.000 description 10
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 9
- 238000003556 assay Methods 0.000 description 9
- 238000002955 isolation Methods 0.000 description 9
- 238000000926 separation method Methods 0.000 description 9
- 239000002023 wood Substances 0.000 description 9
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 8
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 8
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 244000061456 Solanum tuberosum Species 0.000 description 7
- 235000002595 Solanum tuberosum Nutrition 0.000 description 7
- 235000006708 antioxidants Nutrition 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 241000315067 Hymenopellis radicata Species 0.000 description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 6
- 238000012258 culturing Methods 0.000 description 6
- 238000000605 extraction Methods 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 238000005286 illumination Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 4
- 108010000912 Egg Proteins Proteins 0.000 description 4
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 229910052742 iron Inorganic materials 0.000 description 4
- 239000011777 magnesium Substances 0.000 description 4
- 229910052749 magnesium Inorganic materials 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 210000004681 ovum Anatomy 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 230000005070 ripening Effects 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 229930003451 Vitamin B1 Natural products 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 3
- 235000019341 magnesium sulphate Nutrition 0.000 description 3
- 230000013011 mating Effects 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 229960003495 thiamine Drugs 0.000 description 3
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 3
- 239000011691 vitamin B1 Substances 0.000 description 3
- 235000010374 vitamin B1 Nutrition 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 206010002660 Anoxia Diseases 0.000 description 2
- 241000976983 Anoxia Species 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 206010021143 Hypoxia Diseases 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 230000007953 anoxia Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 210000000476 body water Anatomy 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 206010012601 diabetes mellitus Diseases 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 239000011121 hardwood Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000036542 oxidative stress Effects 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 241000222485 Agaricales Species 0.000 description 1
- 241001660920 Agrocybe chaxingu Species 0.000 description 1
- 201000009030 Carcinoma Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241001313708 Dictyophora phalloidea Species 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 241000446313 Lamella Species 0.000 description 1
- JSCQSBGXKRTPHZ-UHFFFAOYSA-N Mucidin Natural products COC=C(C(=O)OC)C(C)=CC=CC1=CC=CC=C1 JSCQSBGXKRTPHZ-UHFFFAOYSA-N 0.000 description 1
- 241000993286 Mucidula mucida Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 208000019155 Radiation injury Diseases 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 208000006268 Sarcoma 180 Diseases 0.000 description 1
- JSCQSBGXKRTPHZ-WMSPMLHYSA-N Strobilurin A Natural products COC=C(/C(=C/C=C/c1ccccc1)/C)C(=O)OC JSCQSBGXKRTPHZ-WMSPMLHYSA-N 0.000 description 1
- 241000434037 Volva Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000000496 anti-anoxic effect Effects 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000006356 dehydrogenation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- JSCQSBGXKRTPHZ-SYKZHUKTSA-N mucidin Chemical compound CO\C=C(\C(=O)OC)/C(/C)=C\C=C\C1=CC=CC=C1 JSCQSBGXKRTPHZ-SYKZHUKTSA-N 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 210000004493 neutrocyte Anatomy 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 230000003617 peroxidasic effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000003642 reactive oxygen metabolite Substances 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical class OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H15/00—Fungi; Lichens
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Epidemiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Biotechnology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Developmental Biology & Embryology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The present invention relates to a kind of new edible fungus species and its cultivation more particularly to a kind of thick pleat Aode mushroom novel bacterial, cultural method and application thereof.The thickness pleat Aode mushroom novel bacterial is thickness pleat Aode mushroom Oudemansiella crassifolia HMGIM S140148.The beneficial effects of the invention are as follows：The present invention provides a kind of thick pleat Aode mushroom novel bacterial, and additionally provides corresponding artificial cultivation method.The artificial cultivation of the thickness pleat Aode mushroom is stable, cultivating rate is high, delicious flavour, and after testing, active constituent content is abundant, and antioxidation is strong, has good economic benefit and social benefit.
Description
Technical field
The present invention relates to a kind of new edible fungus species and its cultivation more particularly to a kind of thick pleat Aode mushroom novel bacterial, plant
Culture method and application thereof.
Background technology
Thick pleat Aode mushroomOudemansiella crassifolia, also known as wide pleat oudemansiella radicata belongs to mycota, load
Bacterium door, Agaricales, bubble head Cordycepps, Genus Oudeman Siella Spec.Thick pleat Aode mushroomOudemansiella crassifolia By Corner
It acquired and delivered for the first time in Malaysia in 1994（Corner, Gdns'Bull., Singapore, 1994）, then exist
China was found and was recorded in 2009 by Yang Zhu is good.
The industry development of edible mushroom is swift and violent at present, and the annual yield of China edible mushroom reaches 20,000,000 tons or more, accounts for the whole world
70% or more.In today that mushroom industry flourishes, more and more Rare edible fungus kinds progress into regarding for people
Open country, many original rare kinds are gradually tamed, such as dictyophora phalloidea, Agrocybe chaxingu, from pleat umbrella.Genus Oudeman Siella Spec has a product more than 30
Kind, wherein it is most edible, and it is rich in nutritional ingredient and functional component, individual plants such as viscous oudemansiella radicata (O. mucida) can generate viscous mushroom rhzomorph (mucidin), Antagonistic Fungus, the inhibiting rate to small white mouse sarcoma 180, ehrlich carcinoma is respectively
80% and 90%, significant effect.Therefore Genus Oudeman Siella Spec has in edible mushroom, medicinal fungus domesticating and cultivating and health products developmental research
Very wide foreground.Thick pleat Aode mushroom belongs to meat agaric, and delicious flavour is crisp in taste, and development prospect is preferable.Meanwhile
It belongs to the raw bacterium of wood, and yield is higher, cultivated character is stablized, the thicker shelf-stable of meat, and industrialization prospect is good.
Invention content
In a first aspect, the present invention provides a kind of thick pleat Aode mushroom novel bacterial, which acquires from Zhaoqing Guangdong Dinghu Hill state
Family's grade nature reserve area, is identified as thick pleat Aode mushroom novel bacterial, is named as thick pleat Aode mushroomOudemansiella crassifoliaHMGIM-S140148 was preserved in China typical culture collection center on March 7th, 2016（Referred to as
CCTCC, address are：Wuhan City, Hubei Province Wuchang District Bayi Road Luo Jia Shan）, deposit number is CCTCC M 2016088.
Second aspect, the present invention provide a kind of cultural method of above-mentioned thick pleat Aode mushroom novel bacterial, including tissue separation bacterium
Kind, parent species are made, original seed is made, make production kind, cultivation culture and management of producing mushroom.
The culture medium of the tissue separation strain of the present invention is isolation medium of the inventor through groping repeatedly, according to matter
It is sawdust wheat bran juice 18-22% to measure percentage, preferably 20%, agar 1.8-2.2%, preferably 2%, remaining is water.
Wherein, the preparation method of sawdust wheat bran juice is：By sawdust：Wheat bran：Water is according to volume ratio 1.5-2.5:0.8-1.2:
9-11 is mixed, preferred volume ratio 2：1：10, it boils 10-30 minutes, obtains sawdust wheat bran juice, sawdust is more preferably broad leaf tree
Sawdust.
The third aspect, the present invention also provides above-mentioned thick pleat Aode mushroom novel bacterials to prepare anti-oxidation medicine/antioxidant food
In purposes, the extract or zymotic fluid of especially thick pleat Aode mushroom novel bacterial are in preparing anti-oxidation medicine/antioxidant food
Purposes.
The beneficial effects of the invention are as follows：The present invention provides a kind of thick pleat Aode mushroom novel bacterial, and additionally provides corresponding
Artificial cultivation method.The artificial cultivation of the thickness pleat Aode mushroom is stable, cultivating rate is high, delicious flavour, and after testing, active ingredient
Rich content, antioxidation is strong, has good economic benefit and social benefit.
Specific implementation mode
Thick pleat Aode mushroom novel bacterial
A kind of thick pleat Aode mushroom novel bacterial provided by the invention, is isolated from the national level conservation of nature of Zhaoqing Guangdong Dinghu Hill
Area is thick pleat Aode mushroom novel bacterial through molecular biology identification and Morphological Identification, by organizing isolated original strain, life
Entitled thickness pleat Aode mushroomOudemansiella crassifoliaHMGIM-S140148, on March 7th, 2016 is preserved in
State's Type Tissue Collection（Abbreviation CCTCC, address are：Wuhan City, Hubei Province Wuchang District Bayi Road Luo Jia Shan）, preservation volume
Number be CCTCC M 2016088.
The DNA of fruiting body genome of the thick pleat Aode mushroom novel bacterial CCTCC M 2016088 of the present invention is extracted,
Identification is compared in PCR amplification ITS sequence, finds and thick pleat Aode mushroomOudemansiella crassifoliaSimilitude is high
Up to 95% or more, by Morphological Identification, qualification result is thick pleat Aode mushroomOudemansiella crassifoliaNovel bacterial.
The mode of appearance of the thick pleat Aode mushroom novel bacterial CCTCC M 2016088 of the present invention is characterized as：Fructification as low as in
It is round Deng, bacteria cover diameter 18-38mm, start slightly to put down to protrusion, it is rear slight flat, it is sometimes slightly concave.Cap sallow is to yellowish-brown
Color, edge are in yellowish-white to lark, are glued when moist.Bacterial context yellow-white, cap center thickness 1.5-3 mm.Lamella edge omits bulging,
Nearly growing straight, yellowish white, 4-6 pieces per cm arrange closely to slightly sparse.Stem 12-30 × 1.5-5mm, it is cylindrical, it is accidental close
Cylinder, raw in stem, threadiness, bending, surface yellow-white to orange grey, asepsis ring, stem base portion slightly expand, no volva.
The amino acid content of the thick pleat Aode mushroom novel bacterial CCTCC M 2016088 of the present invention is high, and magnesium, iron and Zn content are rich
It is rich.
The cultural method of thick pleat Aode mushroom novel bacterial
The present invention also provides the cultural methods of thickness pleat Aode mushroom novel bacterial CCTCC M 2016088 a kind of, including tissue point
From strain, parent species are made, make original seed, make production kind, cultivation culture and management of producing mushroom.
Tissue separation strain is the first step for carrying out novel bacterial cultivation, and the culture medium for carrying out tissue separation strain detaches training
Foster base is that inventor's repetition test obtains, and isolation medium is sawdust wheat bran juice 18-22% according to mass percent, preferably
20%, agar 1.8-2.2%, preferably 2%, remaining is water.
Sawdust wheat bran juice in above-mentioned isolation medium is to be boiled to obtain using sawdust, wheat bran and water, excellent at some
In the embodiment of choosing, the preparation of sawdust wheat bran juice is by by sawdust：Wheat bran：Water is according to volume ratio 1.5-2.5:0.8-1.2:
9-11 is mixed, preferred volume ratio 2：1：10,10-30 minutes, such as 20 minutes are boiled, sawdust wheat bran juice is obtained, sawdust is further
Preferably hardwood sawdust, such as Qinggang sawdust.
In one preferred embodiment, the preparation of isolation medium passes through：By hardwood sawdust such as Qinggang sawdust with
Wheat bran and water are according to volume ratio 1.5-2.5:0.8-1.2:9-11 is mixed, preferred volume ratio 2：1：10, after boiling 10-30 minutes,
Agar is added and is dissolved, sterilizing obtains isolation medium.
After obtaining isolation medium, strain is seeded to isolation medium, in 25 DEG C of ± 1 DEG C of constant temperature incubations, waits for mycelia
Tissue separation strain is completed after covering with inclined-plane, the making parent species step of next step can be carried out.
In making parent species step, the culture medium for making parent species is comprehensive PDA culture medium, comprehensive PDA culture medium Potato
Dextrose Agar（Medium）It is the abbreviation of potato dextrose agar, is a kind of common culture medium, Ke Yi
It is commercially available on the market, a kind of comprehensive PDA culture medium of present invention offer is potato 18-20%, glucose by mass percentage
1.5-2%, agar 2-2.5%, potassium dihydrogen phosphate 0.15-0.3%, magnesium sulfate 0.05-0.15%, vitamin B1 are micro, remaining is water.
In one preferred embodiment, comprehensive PDA culture medium is potato 20%, glucose by mass percentage
2%, agar 2%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, vitamin B1 are micro, remaining is water.
After making comprehensive PDA culture medium, the strain of separation is accessed in comprehensive PDA culture medium, it is dark in 25 DEG C of ± 1 DEG C of constant temperature
Culture, completes the production parent species, it is generally the case that mycelia covers with the time of PDA culture medium 7 after mycelia covers with PDA culture medium
It is between -15 days.
After parent species complete, need to carry out original seed making, the original seed material that original seed makes is cotton according to mass percent
Seed shell 38-43%, preferably 40%, sawdust 35-40%, preferably 38%, wheat bran 18-20%, preferably 20%, calcium carbonate 1-2%, preferably 2%.
In one preferred embodiment, the group of original seed material is divided into：According to 40% cotton seed hulls of mass percent meter, 38% wood
Bits, 20% wheat bran, 2% calcium carbonate.
Original seed make need the parent species of preparation are seeded in original seed material bag, original seed material bag by by original seed material at packing
Enter in strain bag, such as in polypropylene strain bag, burrow in original seed material, burrows in original seed material for example, by using small wood,
Sack lantern ring and lid obtain original seed material bag.
It is routine operation in edible fungus culturing to prepare original seed material bag using original seed material, and cultivating method for edible fungi may be used
In some other routine the step of preparing original seed material bag carry out.
In one preferred embodiment, a kind of method making original seed material bag is provided, according to original seed material component ratio
Cotton seed hulls is weighed, it is wet through bubble, for example (,) soaked overnight, then it is mixed into sawdust, wheat bran, calcium carbonate by component ratio, it is packed into polypropylene
It in strain bag, is burrowed in Bag Material with small wood after installing material, hole is deep to a bag bottom, then covers upper plastic hoop in sack, buckles and match
The lid of set, sterilizing, obtains original seed material bag.
In one preferred embodiment, the polypropylene strain bag is the heat safe polypropylene bacterium of 13cm × 25cm
Bag is planted, the amount that original seed material is packed into each strain bag is equivalent siccative 250g-300g.
The parent species of making are inoculated in original seed material, it is complete after mycelia covers with original seed material in 25 DEG C of ± 1 DEG C of constant temperature light cultures
It is made at original seed is made, in one preferred embodiment, mycelia covers with the time of original seed material between -35 days 25 days.
After original seed completes, production kind can be carried out and made, the production kind material that production kind makes is according to quality percentage
Than for cotton seed hulls 38-43%, preferably 40%, sawdust 35-40%, preferably 38%, wheat bran 18-20%, preferably 20% are calcium carbonate 1-2%, excellent
Select 2%.
In one preferred embodiment, the group of production kind material is divided into：40% cotton seed hulls, 38% wood by mass percentage
Bits, 20% wheat bran, 2% calcium carbonate.
Production kind makes needs and the original seed of preparation is seeded to production kind material bag, the preparation of production kind material bag and original seed material bag
Preparation method it is almost the same, by will produce kind material at being distributed into strain bag, such as in polypropylene strain bag, producing
It burrows in kind material, burrows in production kind material for example, by using small wood, in sack lantern ring and lid, obtain producing kind of a material bag.
It is routine operation in edible fungus culturing to prepare production kind material bag using production kind material, and edible fungus culturing may be used
Some other conventional the step of preparing production kind material bag in method, carries out.
In one preferred embodiment, cotton seed hulls is weighed according to production kind material component ratio, it is wet through bubble, such as soak
Bubble overnight, then is mixed into sawdust, wheat bran, calcium carbonate by component ratio, is fitted into polypropylene strain bag, is existed with small wood after installing material
It burrows in Bag Material, hole is deep to a bag bottom, then covers upper plastic hoop in sack, buckles mating lid, sterilize, obtains production kind bag
Material.
In one preferred embodiment, the polypropylene strain bag is the heat safe polypropylene bacterium of 15cm × 30cm
Kind of bag, it is equivalent siccative 350g-400g to be packed into an amount for production kind material in each strain bag.
The original seed of making is inoculated in production kind material, in 25 DEG C of ± 1 DEG C of constant temperature light cultures, waits for that mycelia covers with production kind material
Afterwards complete production kind make, in one preferred embodiment, mycelia cover with production kind material time -35 days 25 days it
Between.
Be prepared production kind after, can will production kind access a cultivation material bag carry out cultivation culture, 25 DEG C ± 1 DEG C,
Relative air humidity 60-70% is protected from light culture, after the mycelia in cultivation material bag covers with culture material, continues shading After-mature cultivation extremely
Mycelia completes latter stage of ripening.
In one preferred embodiment, between the incubation time that the mycelia covers with culture material is about -35 days 25 days,
Continuation shading After-mature cultivation completion latter stage of ripening, is characterized as that bacteria stick starts to switch to band elasticity, and mycelia has the small former base of kink to start
Existing, the time of the shading After-mature cultivation is about -18 days 12 days, further for example, -15 days 14 days.
The production method of cultivation material bag is identical as the production method of original seed material bag, by by culture material at being distributed into strain
It in bag, such as in polypropylene strain bag, burrows, burrows in production kind material for example, by using small wood, in sack in culture material
Lantern ring and lid, obtain cultivation material bag.
Culture material is cotton seed hulls 48-52%, preferably 50%, sawdust 25-30%, preferably 30%, wheat bran 18- according to mass percent
20%, preferably 18%, calcium carbonate 1-2%, preferably 1%, calcium sulfate 1-2%, preferably 1%.
In one preferred embodiment, the group of culture material is divided into：50% cotton seed hulls, 30% wood by mass percentage
Bits, 18% wheat bran, 1% calcium carbonate, 1% calcium sulfate.
It is routine operation in edible fungus culturing to prepare cultivation material bag using culture material, and cultivating method for edible fungi may be used
In some other routine the step of preparing cultivation material bag carry out.
In one preferred embodiment, cotton seed hulls is weighed according to culture material component ratio, it is wet through bubble, such as impregnate
Overnight, then it is mixed into sawdust, wheat bran, calcium carbonate, calcium sulfate by component ratio, be fitted into polypropylene strain bag, installed after expecting with small
Wooden stick burrows in Bag Material, and hole is deep to a bag bottom, then covers upper plastic hoop in sack, buckles mating lid, sterilize, cultivated
Material bag.
In one preferred embodiment, the polypropylene strain bag is the heat safe polypropylene bacterium of 17cm × 35cm
Kind of bag, it is equivalent siccative 450g-500g to be packed into an amount for production kind material in each strain bag.
After the completion of After-mature cultivation, so that it may to carry out management of producing mushroom, adjustment temperature 25-28 to thick pleat Aode mushroom novel bacterial
DEG C, relative humidity 85-90%, daily illumination 10 hours, intensity of illumination 300-500lx, and keep the carbon dioxide concentration in air small
In 2%, keep with the humid air, when growing young mushroom, spray water 1-2 time to young mushroom daily, up to fructification cap from it is interior receive it is flat
It stretches, harvests.
In one preferred embodiment, the cultivating bag after harvesting can also continue to shading After-mature cultivation until young mushroom again
It is secondary to grow and harvest, continue shading After-mature cultivation until the condition of culture that young mushroom grows and harvests again was cultivated and adopted with first time
It is identical or different to receive thick pleat Aode mushroom, for example, the cultivation material bag after harvesting is positioned over 25 DEG C ± 1 DEG C, relative air humidity 60-
After-mature cultivation is completed in shading in 70% culturing room, then adjusts 25-28 DEG C of temperature, relative humidity 85-90%, and daily illumination 10 is small
When, intensity of illumination 300-500lx, and the carbon dioxide concentration in air is kept to be less than 2%, keep with the humid air, when growing young mushroom
When, it sprays water 1-2 times to young mushroom daily, until fructification cap receives flattened, harvesting from interior.
In one preferred embodiment, the cultivation material bag for completing After-mature cultivation needs the lid for opening cultivation material bag,
And cultivation material bag vertical setting of types is placed, management of producing mushroom is carried out.
In one preferred embodiment from young mushroom is grown to fructification maturation（Fructification cap from it is interior receive it is flattened）
About pass through 2-6 days.
The purposes of thick pleat Aode mushroom novel bacterial
The present invention also provides a kind of purposes of thick pleat Aode mushroom novel bacterial, are used for anti-oxidant or anti anoxia.
Tests prove that thick pleat Aode mushroom novel bacterial has antioxidant activity, including strong reducing power, hydroxy radical outstanding
(- OH) cleaning performance and superoxide radical O2Rejection, for example, thick pleat Aode mushroom novel bacterial extract, thick pleat Aode mushroom
The zymotic fluid of novel bacterial.
The extract of thick pleat Aode mushroom novel bacterial, the zymotic fluid of thickness pleat Aode mushroom novel bacterial may be used to anti-oxidant or anti-
Anoxic specifically can be used for treating oxidation resistant disease or the disease of anti anoxia, such as be used to prepare treatment antioxidant disease
Drug or for oxidation resistant food/health products, such as angiocardiopathy, diabetes etc..
The extract of thick pleat Aode mushroom novel bacterial can be the mycelial extract of thick pleat Aode mushroom novel bacterial, using solvent
Thick pleat Aode mushroom novel bacterial mycelium is extracted to obtain, solvent can select alcohol（Such as methanol, ethyl alcohol, isopropanol）, water, ester
（Such as ethyl acetate）, ketone（Such as acetone）Deng or these solvents mixture.
In one preferred embodiment, a kind of mycelial water extract of thick pleat Aode mushroom novel bacterial is provided,
By thick pleat Aode mushroom novel bacterial mycelium and water according to mycelium dry weight quality and water volume ratio 1:30 ratio, 80 DEG C of temperature
It extracts, obtains the mycelial water extract of thick pleat Aode mushroom novel bacterial.
In another preferred embodiment, a kind of mycelial ethyl alcohol extraction of thick pleat Aode mushroom novel bacterial is provided
Object, by thick pleat Aode mushroom novel bacterial mycelium and absolute ethyl alcohol according to mycelium dry weight quality and absolute ethyl alcohol volume ratio 1:30
Ratio, 70 DEG C of temperature extract, and obtain the mycelial ethanol extract of thick pleat Aode mushroom novel bacterial.
In another preferred embodiment, the mycelium and zymotic fluid of a kind of thick pleat Aode mushroom novel bacterial are provided
Mode is obtained, thick pleat Aode mushroom novel bacterial is seeded in PDA solid mediums, 28 DEG C of ± 1 DEG C of constant temperature incubation to mycelia are covered with
Mycelium is transferred in PDA culture solutions again after culture medium, after 25 DEG C of ± 1 DEG C of constant-temperature shaking cultures to zymotic fluid are clarified, filtering,
Respectively obtain the zymotic fluid of the mycelium and thick pleat Aode mushroom novel bacterial of thick pleat Aode mushroom novel bacterial.
In another preferred embodiment, the PDA of the mycelium and zymotic fluid that prepare thick pleat Aode mushroom novel bacterial is solid
Body culture medium is containing 200 g of potato, 20 g of glucose, 20 g of agar, 1000 mL of water；PDA culture solutions are containing 200 g of potato, Portugal
20 g of grape sugar, 1000 mL water.
The extract of thick pleat Aode mushroom novel bacterial, the zymotic fluid of thickness pleat Aode mushroom novel bacterial can be used for preparing anti-oxidant disease
Medicine/food/health products, such as a kind of drug of antioxidant disease, including the extract of thick pleat Aode mushroom novel bacterial and/or
The zymotic fluid of thick pleat Aode mushroom novel bacterial and a kind of pharmaceutically acceptable carrier.
Oxidative stress refers to vivo oxidation and antioxidation is unbalance tends to aoxidize, and neutrophil leucocyte inflammatory is caused to soak
Profit, protease secretion increase, and generate a large amount of intermediate oxidation products.Oxidative stress is that one kind for being generated in vivo by free radical is negative
Effect, and be considered as a key factor for leading to aging and disease.Meanwhile the unbalance of vivo oxidation reaction is also to cause to be permitted
The key reason of more diseases such as cardiovascular and cerebrovascular disease, diabetes.
Below in conjunction with specific embodiment, invention is further explained.
Embodiment 1：
The cultural method of thick pleat Aode mushroom novel bacterial CCTCC M 2016088 and obtained fructification
One, cultural method is as follows：
1, matrix manufacturing is cultivated：
（1）Isolation medium is：According to mass percent be Qinggang sawdust wheat bran juice it is 20%, agar 2%, remaining is
Water；
The preparation of isolation medium：By Qinggang sawdust and wheat bran and water according to volume ratio 2：1：10 mixing, boil 20 minutes
Afterwards, agar is added and is dissolved, packing test tube is divided in 0.11MPa atmospheric pressure, 121 DEG C of high temperature and pressure moist heat sterilization 30min
From culture medium.
（2）Mother culture media is：According to mass percent meter potato 20%, glucose 2%, agar 2%, potassium dihydrogen phosphate
0.3%, magnesium sulfate 0.15%, vitamin B1 are micro, remaining is water；
（3）Original seed material is：According to 40% cotton seed hulls of mass percent meter, 38% sawdust, 20% wheat bran, 2% calcium carbonate；
The preparation process of original seed material bag is：Cotton seed hulls is weighed, it is wet overnight through bubble, it is mixed into sawdust, wheat bran, carbonic acid in proportion
Calcium is fitted into the heat safe transparent polypropylene strain bags of 13cm × 25cm, is converted into per packed siccative 250-300g, install after material
Sack puts on plastic hoop, buckles mating lid, cold in 0.147MPa atmospheric pressure, 128 DEG C of high temperature and pressure moist heat sterilization 90min
Spare, obtain the original seed material bag that can directly use；
（4）Production kind is expected：40% cotton seed hulls, 38% sawdust, 20% wheat bran, 2% calcium carbonate by mass percentage；
The manufacturing process of production kind material bag is substantially identical as original seed material bag, but strain bag used is 15cm × 30cm high temperature resistants
Transparent polypropylene strain bag.Equivalent every packed siccative 350-400g；
（5）Culture material is：50% cotton seed hulls, 30% sawdust, 18% wheat bran, 1% calcium carbonate, 1% sulfuric acid by mass percentage
Calcium；
The manufacturing process of cultivation material bag is substantially identical as original seed material bag, but in formula when being mixed into sawdust, wheat bran, calcium carbonate
It is mixed into calcium sulfate together in proportion, strain bag used uses the heat safe transparent polypropylene strain bags of 17cm × 35cm, converts into every
Packed siccative 450-500g；
2, tissue separation strain：Thick 2016088 fructifications of pleat Aode mushroom novel bacterial CCTCC M are seeded to and are separately cultured
25 DEG C of ± 1 DEG C of light cultures of base cover with culture medium to mycelia and obtain the strain of tissue separation substantially；
3, the strain by tissue separation is seeded to mother culture media, and 25 DEG C of ± 1 DEG C of light cultures cover with culture to mycelia substantially
Base obtains parent species, and the time that parent species cover with about needs 7 days to 15 days；
4, parent species are seeded in original seed material bag, it is ensured that parent species are embedded in original seed material, 25 DEG C of ± 1 DEG C of light cultures to mycelia base
Originally it eats full material and obtains original seed, which about needs 30 days or so；
5, original seed is seeded in production material bag, it is ensured that in original seed embedment production material, 25 DEG C of ± 1 DEG C of light cultures to mycelia base
Originally it eats full material and obtains production kind, which about needs 30 days or so；
6, production kind is seeded in cultivation material bag, is protected from light culture in 25 DEG C ± 1 DEG C, relative humidity 60-70%, waits for mycelia
After covering with culture material（The process about needs 30 days or so）, continue shading After-mature cultivation to mycelia and complete latter stage of ripening（The process is about
Need 15 days or so）, completing latter stage of ripening is characterized as that bacteria stick starts to switch to band elasticity, and mycelia has the small former base of kink to start to occur；
7, open cultivating bag lid, the placement of cultivating bag vertical setting of types, between bag and bag there are gap, 25-28 DEG C, it is relatively wet
It spends in the environment of 85-90%, daily illumination 10 hours, intensity of illumination 300-500lx, and keeps the carbon dioxide concentration in air small
It in 2%, keeps with the humid air, when growing young mushroom, sprays water mist 1-2 times to young mushroom daily, until fructification cap is received from interior
It is flattened, illustrate that fructification has become ripe, can harvest at this time, about passes through -5 days 3 days to fructification maturation from young mushroom is grown.
8, the cultivating bag after harvesting can also continue to shading After-mature cultivation until young mushroom grows and harvests again, after harvesting
Cultivation material bag be positioned over 25 DEG C ± 1 DEG C, After-mature cultivation is completed in shading in the culturing room of relative air humidity 60-70%, repeat
Step 7 is to harvesting.
Two, result is cultivated：
1, fruiting phase：The fruiting phase of the strain is up to 2 months, can 3 tide of fruiting；
2, yield：Each mushroom bag is per damp 100 grams or so of fruiting, and overall biological conversion ratio is about 80% or so；
3, fructification character：There is mucus on fructification cap surface, early period dark brown, later stage lighter, handle is 5-10 lis long
Rice, cap are in umbrella shape, and meat is thick, more fertile tender.
Experimental example 1：
The nutrient component determining of thick 2016088 fructifications of pleat Aode mushroom CCTCC M of artificial cultivation
Thick 2016088 fructifications of pleat Aode mushroom CCTCC M and ovum spore oudemansiella radicata obtained to artificial cultivation of the present invention point
Nutrient component determining, including hydrolysis amino acid, polysaccharide, protein and trace element magnesium, iron, Direct spectrophotometry, amino acid are not carried out
Assay method with reference to GB/T 5009.124-2003 methods, the assay method of Thick many candies is with reference to NY/T 1676-2008/7, egg
The assay method of white matter is with reference to GB/T the first methods of 5009.5-2010/, the assay method reference GB/T 5009.90- of magnesium and iron
2003, Direct spectrophotometry method is with reference to GB/T the first methods of 5009.14-2003/, and the results are shown in Table 1 for measurement.
Table 1：The nutritional ingredient of thick 2016088 fructifications of pleat Aode mushroom CCTCC M and ovum spore oudemansiella radicata fructification compares
Table
By the measurement result of table 1 it is found that isolating protein content quite other than, thick pleat Aode mushroom CCTCC M's 2016088
Nutrient composition content is all higher than the nutrient composition content of ovum spore oudemansiella radicata, especially thickness pleat Aode mushroom CCTCC M substantially
2016088 hydrolysis amino acid summation is much larger than ovum spore oudemansiella radicata, abundant containing magnesium, iron and Zn content.
Experimental example 2：
The reducing power of thick pleat Aode mushroom novel bacterial CCTCC M 2016088 measures
The reducing power of thick pleat Aode mushroom CCTCC M 2016088 and ganoderma lucidum to artificial cultivation of the present invention are measured.
One, assay method
1, prepared by thick pleat Aode mushroom and the zymotic fluid of ganoderma lucidum, extract
（1）Culture medium：PDA solid mediums are containing 200 g potatos, 20 g glucose, 20 g agar, 1000 mL water；
PDA liquid medium is containing 200 g potatos, 20 g glucose, 1000 mL water；
（2）Zymotic fluid and mycelial preparation：Thick pleat Aode mushroom CCTCC M 2016088 and ganoderma lucidum are transferred to respectively
In PDA solid mediums, 28 DEG C of constant temperature incubations are placed in after mycelia is covered with（About 5-7 days）, it is inoculated with 5 pieces respectively and cultivates consolidating for mycelia
In body culture medium (cm of 0.5 cm × 0.5) to 250 mL bottles containing 100mL PDA liquid mediums, it is placed in 25 DEG C of constant temperature and shakes
In bed, in 110 r min-1Shaken cultivation is taken out after zymotic fluid clarification, respectively obtains fermentation with 100 mesh filter-cloth filterings respectively
Liquid and mycelium；
（3）The preparation of water extract：The mycelium of thick pleat Aode mushroom CCTCC M 2016088 and ganoderma lucidum are blotted into water respectively
Point and drying to constant weight in 60 DEG C, after liquid nitrogen grinding, take 1.5g distillation floodings respectively, extraction conditions are that solid-liquid ratio is 1:
30（The volume ratio of mass volume ratio, i.e. mycelium dry weight and distilled water）, 80 DEG C of Extracting temperature, 2 hours extraction times, be settled to
50 mL respectively obtain the water extract of thick pleat Aode mushroom CCTCC M 2016088 mycelial water extract and ganoderma lucidum；
（4）The preparation of ethanol extract：The mycelium of thick pleat Aode mushroom CCTCC M 2016088 and ganoderma lucidum are blotted respectively
Moisture and drying to constant weight in 60 DEG C, after liquid nitrogen grinding, takes 1.5g to be extracted with absolute ethyl alcohol, extraction conditions are solid-liquid ratio respectively
It is 1: 30（The volume ratio of mass volume ratio, i.e. mycelium dry weight and absolute ethyl alcohol）, 80 DEG C of Extracting temperature, extraction time 2 is small
When, 50 mL are settled to, the second of thick pleat Aode mushroom CCTCC M 2016088 mycelial ethanol extract and ganoderma lucidum is respectively obtained
Alcohol extracting thing.
2, the measurement of reducing power
Assay method：Take 0.2 mol L-1Phosphate buffer (pH6.8) and 1% K3Fe(CN)6Each 2.5 mL is added 2
The certain density extract of mL（Prepare liquid）, in 50 DEG C of 30 min of water-bath after shaking up, 2.5 mL10% trichloroacetic acids are added, in
4000 r• min-110 min are centrifuged, supernatant 2.5mL and 2.5 mL distilled water and 0.5 mL0.1% FeCl are taken3Mixing, in
Optical density D is measured at 700 nm.
It is calculated by the following formula to obtain the reducing power of extract：
The reducing power of extract=DX-DX0-D0
Wherein, the D values of DX- extracts, the background D values of DX0- extracts（The D of the untreated extract directly measured
Value）, the D values of D0- blank control liquid（Make blank control liquid with phosphate buffer）.
Two, measurement result
According to above-mentioned reducing power assay method, measures obtain the thick pleat Aode mushroom CCTCC M of various concentration respectively
2016088 mycelium water extracts, 2016088 mycelium ethanol extracts of thickness pleat Aode mushroom CCTCC M, thick pleat Aode mushroom
The reduction of CCTCC M 2016088 zymotic fluid and ganoderma lucidum mycelium water extract, Ganoderma lucidum mycelium ethanol extract, ganoderma lucidum fermented liquid
Power, as shown in table 2- tables 3.
The reducing power of thick the pleat Aode mushroom and ganoderma lucidum of 2 6mg/ml of table
| Sample | Reducing power | Sample | Reducing power |
| The thick 2016088 mycelium water extracts of pleat Aode mushroom CCTCC M of 6mg/ml | 2.77 | The ganoderma lucidum mycelium water extract of 6mg/ml | 1.375 |
| The thick 2016088 mycelium ethanol extracts of pleat Aode mushroom CCTCC M of 6mg/ml | 1.959 | The ganoderma lucidum mycelium ethanol extract of 6mg/ml | 0.907 |
| Thick 2016088 zymotic fluids of pleat Aode mushroom CCTCC M of 6mg/ml | 0.511 | The ganoderma lucidum fermented liquid of 6mg/ml | 0.137 |
From table 2 it can be seen that either mycelium water extract, ethanol extract or the zymotic fluid of same concentrations, thick
Pleat Aode mushroom CCTCC M 2016088 are significantly larger than the reducing power of ganoderma lucidum, especially thickness pleat Aode mushroom CCTCC M 2016088
The reducing power of mycelium water extract is most strong.
The reducing power of the thick pleat Aode mushroom of 3 3mg/ml of table and the thick pleat Aode mushroom of 2mg/ml
| Sample | Reducing power | Sample | Reducing power |
| The thick 2016088 mycelium water extracts of pleat Aode mushroom CCTCC M of 3mg/ml | 1.167 | The thick 2016088 mycelium water extracts of pleat Aode mushroom CCTCC M of 2mg/ml | 0.901 |
| The thick 2016088 mycelium ethanol extracts of pleat Aode mushroom CCTCC M of 3mg/ml | 1.027 | The thick 2016088 mycelium ethanol extracts of pleat Aode mushroom CCTCC M of 2mg/ml | 0.684 |
| Thick 2016088 zymotic fluids of pleat Aode mushroom CCTCC M of 3mg/ml | 0.207 | Thick 2016088 zymotic fluids of pleat Aode mushroom CCTCC M of 2mg/ml | 0.136 |
From table 3 it can be seen that under same concentrations, thick 2016088 mycelium water extracts of pleat Aode mushroom CCTCC M are also
Former power is most strong, and the reducing power of thick 2016088 mycelium ethanol extracts of pleat Aode mushroom CCTCC M takes second place, thick pleat Aode mushroom
The reducing power of 2016088 zymotic fluids of CCTCC M is lower, and certain positive correlation, identical extraction is presented with concentration in reducing power
Object or zymotic fluid, concentration is higher, and reducing power is stronger.
Experimental example 3:
The measurement of the inhibition hydroxyl radicals of thick pleat Aode mushroom novel bacterial CCTCC M 2016088
Assay method：Using hydroxy radical assay kit（Nanjing is built up）, difference thickness measuring pleat Aode mushroom CCTCC M
2016088 mycelium water extracts, 2016088 mycelium ethanol extracts of thickness pleat Aode mushroom CCTCC M, thick pleat Aode mushroom
The inhibition of CCTCC M 2016088 zymotic fluid and ganoderma lucidum mycelium water extract, Ganoderma lucidum mycelium ethanol extract, ganoderma lucidum fermented liquid
Hydroxyl radicals, the results are shown in Table 4.
Table 4 inhibits hydroxyl radicals result
| Sample | Inhibit hydroxyl radicals (U/ml) | Sample | Inhibit hydroxyl radicals (U/ml) |
| Thick 2016088 mycelium water extracts of pleat Aode mushroom CCTCC M | 1520.76 | Ganoderma lucidum mycelium water extract | 1458.45 |
| Thick 2016088 mycelium ethanol extracts of pleat Aode mushroom CCTCC M | 129.71 | Ganoderma lucidum mycelium ethanol extract | 77.61 |
From table 4, it can be seen that the mycelium water extract and ethanol extract of thick pleat Aode mushroom all have strong removing
The ability of hydroxy radical, elimination effect are even better than ganoderma lucidum.Hydroxy radical（-OH）For high response free radical, electricity can be passed through
The modes such as son transfer, addition and dehydrogenation are acted on the different kinds of molecules in organism, cause carbohydrate, amino acid, protein, nucleic acid
With the oxidative damage of the substances such as lipid, make meronecrosis or mutation.- OH is also gulped down with aging, tumour, radiation injury and cell
It bites etc. related, inhibits hydroxy radical（-OH）Ability is to reflect the important indicator of antioxidation of drug.
Embodiment 4:
The measurement of the superoxide anion resisitance Free Radical Activity unit of thick pleat Aode mushroom novel bacterial CCTCC M 2016088
Assay method：Using superoxide anion resisitance free radical and generate ultra-oxygen anion free radical testing cassete（Nanjing is built up）
It is detected experiment, and calculates superoxide anion resisitance Free Radical Activity unit, to thick 2016088 mycelia of pleat Aode mushroom CCTCC M
The superoxide anion resisitance Free Radical Activity unit of body water extract and 2016088 zymotic fluids of thickness pleat Aode mushroom CCTCC M is surveyed
It is fixed.In reaction system, every liter of sample reacts 40 minutes ultra-oxygen anion free radicals inhibited at 37 DEG C and is equivalent to the dimension of 1mg
The changing value for the ultra-oxygen anion free radical that raw element C is inhibited is a unit of activity.The results are shown in Table 5.
Table 5：Superoxide anion resisitance Free Radical Activity unit results
| Sample | Superoxide anion resisitance Free Radical Activity unit（U/L） |
| Thick 2016088 mycelium water extracts of pleat Aode mushroom CCTCC M | 100.64 |
| Thick 2016088 zymotic fluids of pleat Aode mushroom CCTCC M | 180.69 |
Table 5 the result shows that, superoxide anion resisitance Free Radical Activity unit value is higher, illustrate super oxygen the moon that it is inhibited from
The ability of sub- changes of free radicals is stronger, is a stronger performance of inoxidizability, thick 2016088 mycelia of pleat Aode mushroom CCTCC M
Body water extract and zymotic fluid all have superoxide anion resisitance free radical activity outstanding.
In biological vivo oxidation reduction reaction, substantially there is 2%~5% oxygen to will produce ultra-oxygen anion free radical, O2
Electronq donor is made, and can receive electronics, chemical property is very active.O2It goes back decomposable asymmetric choice net and forms stronger reactive oxygen species, such as
Singlet oxygen and-OH generate peroxidatic reaction of lipid, moreover it is possible to H2O2Form generation-OH precursor, to causing fat indirectly
Matter peroxidating.So it is also the antioxidative big event of evaluation to inhibit the ability of superoxide radical.
In conclusion the thick pleat Aode mushroom CCTCC M 2016088 of the present invention have strong anti-oxidation, especially mycelium
The reducing power of water extract and the ability of scavenging hydroxyl, superoxide anion resisitance Free Radical Activity are very prominent, in edible medicinal
Bacterium even has exceeded the strong ganoderma lucidum of inoxidizability.
The above, only preferable specific embodiment of the invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Design is subject to equivalent substitution or change, should all cover within the scope of the present invention.
Claims (4)
1. a kind of thickness pleat Aode mushroom strain, which is characterized in that the thickness pleat Aode mushroom strain is thick pleat Aode mushroomOudemansiella crassifoliaHMGIM-S140148, deposit number are CCTCC NO：M 2016088.
2. a kind of use of thick pleat Aode mushroom strain according to claim 1 in preparing anti-oxidation medicine/antioxidant food
On the way.
3. the extract or zymotic fluid of a kind of thick pleat Aode mushroom strain according to claim 1 prepare anti-oxidation medicine/
Purposes in antioxidant food.
4. purposes according to claim 3, which is characterized in that the extract is the mycelial of thick pleat Aode mushroom strain
Extract, the extract are water extract, alcohol extracting thing, ester extract, ketone extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610658412.3A CN106258999B (en) | 2016-08-11 | 2016-08-11 | A kind of thickness pleat Aode mushroom strain, cultural method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610658412.3A CN106258999B (en) | 2016-08-11 | 2016-08-11 | A kind of thickness pleat Aode mushroom strain, cultural method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106258999A CN106258999A (en) | 2017-01-04 |
| CN106258999B true CN106258999B (en) | 2018-08-31 |
Family
ID=57668636
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610658412.3A Active CN106258999B (en) | 2016-08-11 | 2016-08-11 | A kind of thickness pleat Aode mushroom strain, cultural method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106258999B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110754292B (en) * | 2019-11-25 | 2021-08-17 | 广东省微生物研究所（广东省微生物分析检测中心） | White variety of oospore oudemansiella mucida and artificial cultivation method thereof |
| CN111937680B (en) * | 2020-06-17 | 2022-03-25 | 广东省微生物研究所（广东省微生物分析检测中心） | New spawn of oospore oudemansiella mucida, artificial cultivation method and application thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630481B (en) * | 2012-04-11 | 2013-07-17 | 广东省微生物研究所 | Cultivation method for oospore oudemansiella mucida |
| CN102907257B (en) * | 2012-10-31 | 2014-05-07 | 北京市农林科学院 | Cultivation method of hazel oudemansiella mucida and special culture medium thereof |
| CN103650914B (en) * | 2013-12-05 | 2016-01-20 | 上海市农业科学院 | A kind of soilless culture method of Aode mushroom |
| CN104446822A (en) * | 2014-12-19 | 2015-03-25 | 广东省微生物研究所 | Special culture medium for oudemansiella canarri (jungh.) hohnel and oudemansiella canarri (jungh.) hohnel cultivation method |
| CN105062899B (en) * | 2015-08-26 | 2017-12-05 | 海南大学 | One plant of two spore intends the extraction and application of Aode mushroom bacterial strain and its Thick many candies |
| CN105769938B (en) * | 2016-04-19 | 2019-11-08 | 北京市农林科学院 | One plant of light brown Aode mushroom and its application |
-
2016
- 2016-08-11 CN CN201610658412.3A patent/CN106258999B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN106258999A (en) | 2017-01-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103330258B (en) | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof | |
| CN106978353B (en) | Te Shi Xylaria sp. fungus and its cultural method | |
| WO2005116187A1 (en) | Industrial fermenting production process of hirsutezla hepiali chen & shen of anamorphic fungi related to chinese cordyceps sinensis | |
| CN105474995A (en) | Cultivation and domestication method of wild collybia albuminosa | |
| CN107475130B (en) | Thin handle Isaria novel bacterial and its cultural method and purposes | |
| CN104145719A (en) | Cordyceps sinensis mycelium fermentation production method | |
| CN105420115B (en) | It is a kind of for halimasch spore separation, culture culture medium and methods and applications | |
| CN109370914A (en) | A kind of cordyceps sinensis mycelium powder and its production method | |
| CN104206169A (en) | Method for preparing nutrient cereal by cordyceps militaris culture medium | |
| KR101908392B1 (en) | Manufacturing method of alcoholic beverage fermented by mushroom | |
| TWI408226B (en) | Submerged cultivation of pleurotus eryngii mycelia high in ergothioneine content | |
| KR100931526B1 (en) | Fermented food produced by fomes formentarius and a method for producing the same | |
| CN101333550A (en) | Method for preparing CYCLIC DIPEPTIDES compounds and use thereof | |
| CN106258999B (en) | A kind of thickness pleat Aode mushroom strain, cultural method and application thereof | |
| CN109906877A (en) | One kind sticking up squama mushroom novel bacterial and its domesticating cultivation method and application | |
| CN108175040B (en) | Preparation method of millet oat antrodia camphorata mycoplasm health food | |
| CN104403952B (en) | A kind of sclerotium oyster mushroom novel bacterial and its cultural method and application | |
| CN108096478A (en) | A kind of production method of the oat Phellinus paddy bacterium powder rich in flavonoids from phellinus | |
| CN108901587A (en) | A kind of solid culture method of cicada fungus | |
| CN110447457A (en) | A kind of pycnoporus samguineus new strains and its artificial cultivation method and purposes | |
| CN109762745A (en) | A kind of fermentation culture method of Pleurotus tuber-regium and the method that active constituent is extracted from culture | |
| CN102630480B (en) | Iron-rich black fungus and production method thereof | |
| CN109566272A (en) | A kind of cultural method and its culture medium of Morchella esculenta (L.) Pers mycelium | |
| CN104446822A (en) | Special culture medium for oudemansiella canarri (jungh.) hohnel and oudemansiella canarri (jungh.) hohnel cultivation method | |
| CN110117548B (en) | New strain of phellinus linteus as well as artificial cultivation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 510070 58 Guangdong Institute of Microbiology, No. 100 Xianliezhong Road, Guangzhou, Guangdong Province Co-patentee after: GUANGDONG YUEWEI EDIBLE FUNGI TECHNOLOGY Co.,Ltd. Patentee after: GUANGDONG INSTITUTE OF MICROBIOLOGY (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) Address before: 510070 58 Guangdong Institute of Microbiology, No. 100 Xianliezhong Road, Guangzhou, Guangdong Province Co-patentee before: GUANGDONG YUEWEI EDIBLE FUNGI TECHNOLOGY Co.,Ltd. Patentee before: Guangdong Institute of Microbiology |
|
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: 510070 No. 58, courtyard, No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province Patentee after: Institute of Microbiology, Guangdong Academy of Sciences Patentee after: GUANGDONG YUEWEI EDIBLE FUNGI TECHNOLOGY Co.,Ltd. Address before: 510070 58 Guangdong Institute of Microbiology, No. 100 Xianliezhong Road, Guangzhou, Guangdong Province Patentee before: GUANGDONG INSTITUTE OF MICROBIOLOGY (GUANGDONG DETECTION CENTER OF MICROBIOLOGY) Patentee before: GUANGDONG YUEWEI EDIBLE FUNGI TECHNOLOGY Co.,Ltd. |
|
| OL01 | Intention to license declared | ||
| OL01 | Intention to license declared |