A kind of Phellinus health protection tea and preparation method thereof
Technical field
The invention belongs to technical field of health care food, and in particular to a kind of Phellinus health protection tea and preparation method thereof.
Background technique
Phellinus is also known as Sang Chen, mulberry ear, belong to Basidiomycota (Basidiomycota), agaric guiding principle (Agaricomycetes), rust
Leather hole Zoopagales (Hymenochaetales), Hymenochaetaceae (Hymenochaetaceae), fine hole Pseudomonas (Inonotus), because
It is usually grown on Mulberry plant, and fructification is gained the name in yellowish-brown.The earliest medicinal record of Phellinus can trace back to Han dynasty
Classical Chinese medicine Shennong's Herbal just has in the Ming Dynasty's Compendium of Material Medica Phellinus " relieving the five internal organs declare flatus, toxin expelling gas " etc. is medicinal to retouch
It states.Modern research shows that Phellinus polysaccharide is principle active component in Phellinus, has and significantly inhibit growth and metastasis of tumours effect.
In addition, Phellinus also has immunological regulation, liver protection and anti-cirrhosis, anti peroxidation of lipid, anti-mutagenesis, hypoglycemic, anti-pneumonia, antibacterial
And antiinflammation.And Phellinus even if taking for a long time, also makees human body adverse reaction very little to people or experimental animal without any poison pair
With.Due to the huge market demand, the wild Phellinus resource of self-assembling formation faces exhaustion, and the difference of Phellinus and most of edible and medical fungis
It is not slow growth, the artificial culture biological efficiency of Phellinus is relatively low at present, it is difficult to realize large-scale planting.
The invention declared at present is matched such as application No. is 201810571321.5 " a kind of yellow mulberry tea and preparation method thereof "
Fang Chengfen are as follows: 2-6 parts of Phellinus, 1-3 parts of fructus lycii, 1-3 parts of jujube, 1-3 parts of mulberry leaf, 1-3 parts of dried orange peel, 0.5-2 parts of Cordyceps militaris.It should
Patent using Phellinus fructification as primary raw material, there is a problem of it is at high cost, expensive, and each component raw material drying crush after
Finished product is mixed, when edible in this way, by simply brewing, the effective component in Phellinus and Cordyceps militaris can not be made full use of,
Cause the waste of raw material.
Summary of the invention
The purpose of the present invention is to solve above-mentioned technical problems, and a kind of Phellinus health protection tea is provided and preparation method thereof.
The object of the present invention is achieved like this: a kind of Phellinus health protection tea, which is characterized in that in mass ratio by following components
Composition: 10-20 parts of mixed fermentation product of phellinus igniarius mycelium and mulberry leaf, 5-15 parts of phellinus igniarius mycelium extract, Cordyceps militaris extraction
5-10 parts of object, 5-15 parts of vine tea powder.
Further, a kind of Phellinus health protection tea is made of the present invention in mass ratio following components: phellinus igniarius mycelium and
15 parts of the mixed fermentation product of mulberry leaf, 10 parts of phellinus igniarius mycelium extract, 8 parts of Cordyceps militaris extract, 10 parts of vine tea powder.
The invention also includes a kind of above-mentioned Phellinus health protection teas, are made of following steps:
Step 1: phellinus igniarius mycelium and mulberry leaf mixed fermentation
(1) actication of culture
Take Phellinus parent species strain transfer on PDA slant medium, 28 DEG C of incubator dark culture 10d take the growth at edge vigorous
Mycelia is inoculated on new slant medium, transfers 3-4 generation, saves backup;The vigorous mycelia of the growth of inclined-plane top edge is taken to connect again
It plants in PDA fluid nutrient medium, 25 DEG C, to get seed liquor after dark culture 6d under 160 r/min oscillating conditions.
(2) liquid fermentation medium is prepared
Prepare mulberry leaf leaching liquor: the mulberry leaf after taking drying are added to the water according to solid-liquid ratio 1:20, and small fire decocts 30min, filtering
After take filtrate, by filter residue repeat extract 1 time, merge 2 filtrates, be made mulberry leaf leaching liquor;
It prepares culture medium: mulberry leaf leaching liquor being substituted into water, Phellinus liquid fermentation basal medium is prepared, is fitted into fermentor, is filled
Liquid measure is the 60%-70% of tank body volume, spare after sterilization and cooling.
(3) it is inoculated with
Seed liquor, inoculum concentration 10%-15% is added.
(4) it ferments
Fermentation temperature is 28 DEG C, speed of agitator 200r/min, ventilation quantity 0.5VVM, cultivates stopping fermentation after 5d.
(5) separation and fermentation product
Above-mentioned tunning is filtered, collects phellinus igniarius mycelium and fermentation liquid respectively, it is spare.
Step 2: preparing the mixed fermentation product of phellinus igniarius mycelium and mulberry leaf
Fermentation liquid is spray-dried, the mixed fermentation product of phellinus igniarius mycelium and mulberry leaf is obtained, it is spare.
Step 3: preparing phellinus igniarius mycelium extract
After the phellinus igniarius mycelium that liquid fermentation and culture is obtained is broken, water is added, the solution that concentration is 300g/L is made, then plus
Enter the complex enzyme that mass fraction is 3%-7%, after mixing, be added in ultrasonic extractor, selects ultrasonic power 300W, processing temperature
50 DEG C of degree handles time 60min, and to treated, solution is spray-dried, and phellinus igniarius mycelium extract is made, spare.
Step 4: preparation Cordyceps militaris extract
Fruiting bodies of cordyceps militaris grind into powder is taken, 60 meshes is crossed, is added to the water with solid-liquid ratio for the ratio of 80g/L, solution is made,
Then 90 DEG C of water-baths extract 2h, and to treated, solution is spray-dried, and Cordyceps militaris extract is made, spare.
Step 5: preparing vine tea powder
Vine tea grind into powder is taken, 60 meshes are crossed, vine tea powder is made, it is spare.
Step 6: finished product
By the mixed fermentation product of phellinus igniarius mycelium obtained above and mulberry leaf, phellinus igniarius mycelium extract, Cordyceps militaris extract,
Vine tea powder is put into batch mixer, after mixing, is fitted into tea bag, is sterilized, and packaging obtains finished product.
Further, the group of complex enzyme becomes cellulase, papain and pectase to the present invention in the step 3,
Mass ratio is 2:2:1.
Further, the constituent of the fluid nutrient medium is sucrose 30g/L, 20 g/L of corn flour, soya bean to the present invention
10 g/L of powder, potassium dihydrogen phosphate 0.5g/L, magnesium sulfate 0.5g/L, sodium chloride 1g/L, pH value are natural.
The invention has the following beneficial effects:
1, Phellinus fructification is replaced with phellinus igniarius mycelium, alleviates the crisis of Phellinus scarcity of resources, reduce production cost;
2, phellinus liteus liquid fermentation medium is prepared with mulberry leaf leaching liquor substitution water, the production of phellinus igniarius mycelium not only can be improved
Amount, and the active constituent in mulberry leaf can be retained into tunning;
3, phellinus igniarius mycelium and Cordyceps militaris functional polysaccharide extraction process are added, allow the active constituent of Phellinus and Cordyceps militaris by
Effective use;
4, mixed fermentation product, phellinus igniarius mycelium extract, Cordyceps militaris extract and vine tea comprising phellinus igniarius mycelium and mulberry leaf
Powder, compatibility is reasonable, so that the present invention is had hypoglycemic, antitumor, antibacterial anti-inflammatory, oxidation resistant function, immunity of organism can be enhanced
Power improves inferior health, repairs hepar damnification, and without any side effects;
5, phellinus igniarius mycelium fluid nutrient medium of the present invention selects corn flour and sucrose is carbon source, and selections soybean powder is nitrogen source, can be with
The peculiar smell for reducing fermentation liquid makes product have preferable mouthfeel.
Detailed description of the invention
The experimental result picture of Fig. 1 experimental example.
Specific embodiment
The present invention will be further explained with reference to the examples below.
Embodiment 1
A kind of Phellinus health protection tea, is made of in mass ratio following components: 10 parts of mixed fermentation product of phellinus igniarius mycelium and mulberry leaf,
5 parts of phellinus igniarius mycelium extract, 5 parts of Cordyceps militaris extract, 5 parts of vine tea powder.
Embodiment 2
A kind of Phellinus health protection tea, is made of in mass ratio following components: 15 parts of mixed fermentation product of phellinus igniarius mycelium and mulberry leaf,
10 parts of phellinus igniarius mycelium extract, 8 parts of Cordyceps militaris extract, 10 parts of vine tea powder.
Embodiment 3
A kind of Phellinus health protection tea, is made of in mass ratio following components: 20 parts of mixed fermentation product of phellinus igniarius mycelium and mulberry leaf,
15 parts of phellinus igniarius mycelium extract, 10 parts of Cordyceps militaris extract, 15 parts of vine tea powder.
Embodiment 4
A kind of Phellinus health protection tea, is made of following steps:
Step 1: phellinus igniarius mycelium and mulberry leaf mixed fermentation
(1) actication of culture
Take Phellinus parent species strain transfer on PDA slant medium, 28 DEG C of incubator dark culture 10d take the growth at edge vigorous
Mycelia is inoculated on new slant medium, transfers 3-4 generation, saves backup;The vigorous mycelia of the growth of inclined-plane top edge is taken to connect again
It plants in PDA fluid nutrient medium, 25 DEG C, to get seed liquor after dark culture 6d under 160 r/min oscillating conditions.
(2) liquid fermentation medium is prepared
Prepare mulberry leaf leaching liquor: the mulberry leaf after taking drying are added to the water according to solid-liquid ratio 1:20, and small fire decocts 30min, filtering
After take filtrate, by filter residue repeat extract 1 time, merge 2 filtrates, be made mulberry leaf leaching liquor;
It prepares culture medium: mulberry leaf leaching liquor being substituted into water, sucrose 30g/L, 20 g/L of corn flour, 10 g/L of soybean powder, phosphorus is added
Acid dihydride potassium 0.5g/L, magnesium sulfate 0.5g/L, sodium chloride 1g/L, pH value is naturally, prepare Phellinus liquid fermentation basal medium.
Prepared fluid nutrient medium is fitted into fermentor, liquid amount is the 60%-70% of tank body volume, after sterilization and cooling
It is spare.
(3) it is inoculated with
Seed liquor, inoculum concentration 10%-15% is added.
(4) it ferments
Fermentation temperature is 28 DEG C, speed of agitator 200r/min, ventilation quantity 0.5VVM, cultivates stopping fermentation after 5d.
(5) separation and fermentation product
Above-mentioned tunning is filtered, collects phellinus igniarius mycelium and fermentation liquid respectively, it is spare.
Step 2: preparing the mixed fermentation product of phellinus igniarius mycelium and mulberry leaf
Fermentation liquid is spray-dried, the mixed fermentation product of phellinus igniarius mycelium and mulberry leaf is obtained, it is spare.
Step 3: preparing phellinus igniarius mycelium extract
After the phellinus igniarius mycelium that liquid fermentation and culture is obtained is broken, water is added, the solution that concentration is 300g/L is made, then plus
Enter the complex enzyme for cellulase, papain and the pectase composition that mass ratio is 2:2:1, the total quality accounting of complex enzyme is
3%-7%.It after mixing, is added in ultrasonic extractor, selects ultrasonic power 300W, ultrasonication temperature 50 C, ultrasonic wave
Time 60min is handled, solution is spray-dried to treated, and phellinus igniarius mycelium extract is made, spare.
Step 4: preparation Cordyceps militaris extract
Fruiting bodies of cordyceps militaris grind into powder is taken, 60 meshes is crossed, is added to the water with solid-liquid ratio for the ratio of 80g/L, solution is made,
Then 90 DEG C of water-baths extract 2h, and to treated, solution is spray-dried, and Cordyceps militaris extract is made, spare.
Step 5: preparing vine tea powder
Vine tea grind into powder is taken, 60 meshes are crossed, vine tea powder is made, it is spare.
Step 6: finished product
By the mixed fermentation product of phellinus igniarius mycelium obtained above and mulberry leaf, phellinus igniarius mycelium extract, Cordyceps militaris extract,
Vine tea powder is put into batch mixer, after mixing, is fitted into tea bag, is sterilized, and packaging obtains finished product.
Mycelium of the present invention replaces Phellinus fructification, alleviates the crisis of Phellinus scarcity of resources, reduces production cost.This hair
The bright middle mixed fermentation product that phellinus igniarius mycelium and mulberry leaf is added, the extracellular activity of the generation of effective use phellinus igniarius mycelium fermentation
Substance.Mulberry leaf leaching liquor substitutes water and prepares phellinus liteus liquid fermentation medium, and the yield of phellinus igniarius mycelium not only can be improved,
And the active constituent in mulberry leaf can be retained into tunning.Phellinus igniarius mycelium fluid nutrient medium of the present invention selects corn
Powder and sucrose are carbon source, and selection soybean powder is nitrogen source, it is possible to reduce the peculiar smell of fermentation liquid improves product mouthfeel.The present invention includes
The Polyose extraction step of phellinus igniarius mycelium and Cordyceps militaris, in advance extracts functional polysaccharide, and the present invention was being eaten in this way
Cheng Zhong, consumer by simply brewing and can be obtained main functional component, allow the active constituent of Phellinus and Cordyceps militaris by
Effective use.
The present invention further includes pupa worm in addition to the mixed fermentation product of phellinus igniarius mycelium and mulberry leaf, phellinus igniarius mycelium extract
Careless extract and vine tea powder.Wherein, Cordyceps militaris (Cordyceps militaris), alias northern Chinese caterpillar Fungus or Cordceps militaris belong to son
Capsule bacterium.Cordyceps militaris is one of edible medicinal cordyceps sinensis generally acknowledged at present, and cordyceps militaris sporocarp is a variety of rich in cordycepin, polysaccharide, cordycepic acid etc.
Effective component.Polysaccharides in Cultured Cordyceps militaris has heat-resisting, salt, the effect of pH is adjusted, in addition to this also just like hypoglycemic, antitumor, antibacterial
Activity, antioxidant activity, the function of enhancing mononuclear phagocyte system, strengthen macrophages phagocytic capacity, treat hepatopathy toxicity
Infection etc. improves natural kill (NK) cell activity, has bioactivity to body over fatigue, liver reparation etc..Vine tea scientific name
Ampelopsis grossdentata (Ampelopsis grossedentata), belong to Vitaceae Ampelopsis, is a kind of Wild Liane.Rattan
Tea has good health-care effect, and Popular Utilization is with a long history in south China, taste micro-sweet, hardship, cool in nature, swollen to throat is alleviated
Bitterly, liver protecting, refreshment and tranquilization etc. have good application effect.Studies have shown that the antioxygenic property of vine tea is obviously high
In green tea, this is because general flavone content may be up to 41.2% or so in vine tea, wherein dihydromyricetin cellulose content is up to 30% or so.
Modern pharmacology activity research shows that dihydromyricetin has fabulous antioxidant activity, can effectively remove free radical, maintain
Very positive effect is played in body eubolism and physiological hygiene.Therefore, the present invention has hypoglycemic, antitumor, anti-
Bacterium anti-inflammatory, improves the effect of inferior health and reparation hepar damnification at anti-oxidant, enhancing immunity of organisms.
Experimental example
Experimental group is that control group makes using the phellinus liteus fluid nutrient medium in embodiment 4, the difference of control group and experimental group
Fluid nutrient medium substitutes Mori folium extract by water, other processing are all the same.Experimental group and control group respectively set 3 in parallel in fact
It tests.Fluid nutrient medium is fitted into 250mL triangular flask, liquid amount is 100 mL, after the sterile access activation of the inoculum concentration of 10mg
Phellinus parent species mycelia, triangular flask is placed in shaking table and cultivates 6d under 28 DEG C, 160r/min oscillating condition, is centrifugated Phellinus
Mycelium with distilled water repeated flushing, is centrifuged, until cleaning solution no longer has the color of culture medium.Mycelium is placed in
It is dried to permanent quality in 40 DEG C of electric drying oven with forced convection, is weighed after cooling.Experimental result such as Fig. 1.
As seen from Figure 1, the biomass of experimental group is higher than control group, therefore Mori folium extract is conducive to phellinus igniarius mycelium
Growth.
Specific embodiment is to further explanation of the invention rather than limits, and is existed for those of ordinary skills
Further transformation is done in the case where not departing from substantive content of the present invention, and all these transformation all should belong to right appended by the present invention
It is required that protection scope.