CN107455748A - One kind has antioxidation white fungus bird's nest enzyme liquid and preparation method thereof - Google Patents
One kind has antioxidation white fungus bird's nest enzyme liquid and preparation method thereof Download PDFInfo
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- CN107455748A CN107455748A CN201710828926.3A CN201710828926A CN107455748A CN 107455748 A CN107455748 A CN 107455748A CN 201710828926 A CN201710828926 A CN 201710828926A CN 107455748 A CN107455748 A CN 107455748A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention, which provides one kind, has antioxidation white fungus bird's nest enzyme liquid and preparation method thereof, wherein, there is antioxidation white fungus bird's nest enzyme liquid, include the component of following parts by weight：30 50 parts of 30 50 parts of white fungus, 20 30 parts of bird's nest and Opuntia ficus' fruit.The present invention is fermentation raw material from white fungus, the bird's nest rich in animal glycoprotein and Opuntia ficus' fruit rich in vegetable protein rich in fungi polysaccharide, and obtained white fungus bird's nest enzyme liquid has good antioxidation.At the same time, enzyme liquid nutrition made from the fermentation of several material matchings more comprehensively, the more rich uniqueness of taste.
Description
Technical field
The present invention relates to field of functional food, more particularly to it is a kind of have antioxidation white fungus bird's nest enzyme liquid and its
Preparation method.
Background technology
The apoptosis of cell refers to cell programmed death under the regulation and control of gene, and caused active chalcogen is as surpassed in human body
Oxygen anion, hydrogen peroxide and hydroxyl radical free radical etc. are by response to oxidative stress damaging cells DNA, albumen and fat, so as to lead
Cell viability is caused to reduce even apoptosis.
Free radical is supported recently as caused by the increase of people's operating pressure and deterioration of living environment et al. in vivo not
It is disconnected to increase and then cause the speed of function of human body degradation constantly to be accelerated.Research shows that caused oxyradical exists in body
Cellular damage is caused to cause Apoptosis and vigor to decline, while accelerating organism aging process, or coronary heart disease, encephalatrophy and liver are hard
The indirect induced factor of the diseases such as change.Research show due to cardiac muscle cell's aging apoptosis caused by body oxidative damage be cardiac muscle by
The important pathologic process of damage, and the reduction of brain cell and cardiac muscle cell and then the most treasure official generation that result in such cell composition
Such as senile progressive pathologic process of cortex atrophy, myocardial remodelling.At the same time the skin aging that modern people are run into
Trichomadesis etc., there is certain relevance with the increase of vivo oxidation free-radical contents.Oxidation resistant product is mostly in the market
Exogenous antioxidant, it is ineffective and there is certain side effect.
The content of the invention
To solve the above problems, the present invention, which provides one kind, has antioxidation white fungus bird's nest enzyme liquid, including it is following heavy
Measure the component of part：White fungus 40-60 parts, bird's nest 10-30 parts and Opuntia ficus' fruit 10-20 parts.
Further, there is antioxidation white fungus bird's nest enzyme liquid to include following parts by weight：50 parts of white fungus, 20 parts of bird's nest
With 15 parts of Opuntia ficus' fruit.
The present invention also provides a kind of preparation method as described above with inoxidizability effect white fungus bird's nest enzyme liquid, bag
Include following steps：
A, white fungus being ground into Tremella micropowder, the Tremella micropowder toward 1 part of quality adds the water of 40 parts of volumes, soaks 1-2h,
Obtain white fungus soak；
B, the white fungus soak obtained in step A is placed in cooker, at 110-121 DEG C, 0.045-0.105MPa soaks
0.5-1h is carried, obtains leaching liquid of Tremella fuciformims Berk；
C, by fresh Opuntia ficus' fruit deburring remove seed, fresh Opuntia ficus' fruit toward 1 part of quality adds 5 parts of bodies
Long-pending water, the homogenate of Opuntia ficus' fruit is polished into, is mixed with the step B leaching liquid of Tremella fuciformims Berk obtained, obtain white fungus-the operatic circle celestial being
Slap fruit mixed liquor；
D, the white fungus obtained toward step C-Opuntia ficus' fruit mixed liquor adds 10wt%-20wt% sucrose, 110-121
DEG C sterilizing cooling after according to active bacterium number be 1 × 109Cfu/g, inoculum concentration are 50-100g/t inoculating lactic acid bacteriums, in 28-34 DEG C of hair
Ferment 2 months；
E, white fungus dry weight and Opuntia ficus' fruit dry weight ratio contained by accounting for are added in step D into liquid obtained by step D is
The papain of 0.5wt%-1wt% cellulase, 0.5wt%-1wt% pectase and 0.5wt%-1wt%, in
2-5h is digested at a temperature of 45-55 DEG C；
F, enzymolysis liquid obtained by step E is filtered to remove filter residue and maintains 5-10min to make enzyme sudden at 95-100 DEG C filtrate
Go out；
G, bird's nest is ground into (0.5-1.5) cm × (0.5-1.5) cm chips, it is broken obtains bird's nest；Toward the bird's nest of 1 part of quality
The broken middle water for adding 20 parts of volumes, soaks 5-8h at 35-45 DEG C, is filtered to remove not by the moisture of bird's nest absorption and chooses removal of impurities
Matter；
H, in step G resulting materials, the water for the bird's nest 10 parts of volumes of broken middle addition for having absorbed moisture toward 1 part of quality, mixing
Uniformly, the broken dry weight 1wt%-3wt% papains of bird's nest are added, 2-5h is digested at 45-55 DEG C；
I, 10wt%-15wt% sucrose is added into liquid obtained by step H, continuing 5-15min at 101-110 DEG C is gone out
Bacterium, after cooling according to active bacterium number be 1 × 109Cfu/g, inoculum concentration are 50-100g/t inoculating lactic acid bacteriums, in 28-34 DEG C of fermentation
1-2 months, obtain the zymotic fluid containing bird's nest；
J, gained liquid in step I is well mixed to obtain containing the broken white fungus bird's nest ferment of bird's nest with gained liquid in step F
Liquid；
K, 5wt%-10wt% FOSs are added into liquid obtained by step J, are well mixed；
L, it is liquid obtained by step K is filling in carrying out, and duration 10min is sterilized at 101-110 DEG C, packaging,
It is made.
White fungus is Basidiomycetes, and Tremella fungus sporophore, it is praised since ancient times as China traditional medicine feeding habits fungi
For " hat of mushroom ", be many historiography pharmacopeia in China such as《Sheng Nong's herbal classic》、《Compendium of Materia Medica》Deng described in, there is " enriching yin profit
Lung " and other effects, it is first-class excellent tonic product.Modern pharmacology research shows that white fungus is nutritious, and rich in tremella polysaccharides, it can be
Body free radical is removed to a certain extent to slow down body aging, improve body immunity, have radiation injury protective effect and nothing
Side effect.In addition white fungus includes a variety of amino acid needed by human body, and ratio and human body proximity, belongs to high-quality protein
Matter source.White fungus is also rich in multivitamin and mineral element, wherein about containing calcium 350mg, phosphorus in per 100g white fungus dry products
250mg, iron 180mg etc., there is higher edible, medical value.
Bird's nest be the saliva that secrets out of of a variety of birds of Apodidae aerodramus with its eider down be mixed and condensed in grotto depths and
The nest built up, natural bird's nest contain abundant nutriment, including a variety of water-solubility proteins, carbohydrate and calcium, phosphorus,
A variety of mineral elements such as iron, iodine.Bird's nest is considered as a kind of natural rare Chinese medicine since ancient times, has and promotes cell growth, enhancing
Immunity and prevent the multiple efficacies such as influenza virus.Research shows that natural bird's nest is rich in water-solubility protein, and content is up to 59.8-
65.4%, and contain all human essential amino acids, wherein threonine, serine, aspartic acid, proline, glutamic acid, different bright
Propylhomoserin, valine and tyrosine content are generally higher.
Opuntia ficus' fruit is the fruit of opuntia, and it is rich in multiple proteins, amino acid, flavonoid
Various plants nutrient and the antioxidants such as thing, alkaloid, a variety of organic acids and microorganism, its antioxygen viability is compared with vitamin C
It is higher by 7 times.China traditional Chinese medical science pharmacopeia, which records cactus fruit, has the promoting flow of qi and blood circulation, clearing heat and detoxicating, control heart and stomach pain, malaria, ruffian it is swollen,
The multiple efficacies such as burn and scald and snakebite.Clinical trial shows that Opuntia ficus' fruit also has indication wide, evident in efficacy and malicious
The advantages that Small side effects, available for treatment mastitis, parotitis, diabetes, ulcer etc.；Modern pharmacology research shows cactus
Fruit extract has accelerating wound healing, the flexible exquisiteness of skin prevented dry skin, reduced wrinkle and other effects, and it can promote
The diffusion regeneration of human normal fibrocyte, the healing to body interior and superficial wound are all very helpful.
The present invention is from white fungus, the bird's nest rich in animal glycoprotein and the operatic circle rich in vegetable protein rich in fungi polysaccharide
Actually fermentation raw material, obtained white fungus bird's nest enzyme liquid have good antioxidation to prickly pear.At the same time, Ji Zhongyuan
Material arranges in pairs or groups enzyme liquid nutrition made from fermentation more comprehensively, the more rich uniqueness of taste.
A kind of preparation method of white fungus bird's nest enzyme liquid with antioxidation provided by the invention, operating procedure letter
Single, raw material has a variety of biological effects, and raw material ferment excellent taste, is adapted to daily drunk, tool through scientific disposal and rational allocation
Wheat Protein, strengthen immunity of organisms, promote health.
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical scheme be clearly and completely described, it is clear that described embodiment is part of the embodiment of the present invention, rather than
Whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not making creative work premise
Lower obtained every other embodiment, belongs to the scope of protection of the invention.
The embodiment of the present invention, which provides one kind, has antioxidation white fungus bird's nest enzyme liquid, includes the group of following parts by weight
Point：White fungus 40-60 parts, bird's nest 10-30 parts and Opuntia ficus' fruit 10-20 parts.
Further, there is antioxidation white fungus bird's nest enzyme liquid to include following parts by weight：50 parts of white fungus, 20 parts of bird's nest
With 15 parts of Opuntia ficus' fruit.
The embodiment of the present invention also provides a kind of preparation as described above with inoxidizability effect white fungus bird's nest enzyme liquid
Method, comprise the following steps：
A, white fungus being ground into Tremella micropowder, the Tremella micropowder toward 1 part of quality adds the water of 40 parts of volumes, soaks 1-2h,
Obtain white fungus soak；
After white fungus is ground into Tremella micropowder, the relative contact after Tremella micropowder is soaking in stepb with hot water accumulates
White fungus surface lipopolysaccharides and other intracellular beneficiating ingredient quilts of white fungus can be so more beneficial for than not crushing white fungus big index times
It is leached out, extracting efficiency greatly promotes；
B, the white fungus soak obtained in step A is placed in cooker, at 110-121 DEG C, 0.045-0.105MPa soaks
0.5-1h is carried, obtains leaching liquid of Tremella fuciformims Berk；
Hot dipping carries that middle temperature is higher, more violent when being incorporated in the molecular motion of raw material or constitutive material than low temperature, so
It is more beneficial in raw material discharging into water beneficial to molecular dissociation or tremella polysaccharides；
C, by fresh Opuntia ficus' fruit deburring remove seed, fresh Opuntia ficus' fruit toward 1 part of quality adds 5 parts of bodies
Long-pending water, the homogenate of Opuntia ficus' fruit is polished into, is mixed with the step B leaching liquid of Tremella fuciformims Berk obtained, obtain white fungus-the operatic circle celestial being
Slap fruit mixed liquor；
Cactus fruit surface has villiform spinelet, removes not in time, and villiform spinelet is mixed into product with potential
It is dangerous；The purpose of remove seed is conveniently to be beaten fruit, the harder easy damaged mashing machine knife blade of seed；Homogenate principle is broken into step B；
D, the white fungus obtained toward step C-Opuntia ficus' fruit mixed liquor adds 10wt%-20wt% sucrose, 110-121
According to active bacterium number it is 1 × 10 after the cooling of DEG C autoclave sterilization9Cfu/g, inoculum concentration are 50-100g/t inoculating lactic acid bacteriums, in
28-34 DEG C ferments 2 months；
E, white fungus dry weight and Opuntia ficus' fruit dry weight ratio contained by accounting for are added in step D into liquid obtained by step D is
The papain of 0.5wt%-1wt% cellulase, 0.5wt%-1wt% pectase and 0.5wt%-1wt%, in
2-5h is digested at a temperature of 45-55 DEG C
The use of the purpose of cellulase is that cactus fruit contains cellulose (long-chain, molecule are larger), adds cellulase drop
Cellulose is monose (small-molecule substance) in hydrolysis and fermentation liquid, prevents subsequent product because fiber flocculation, which produces precipitation, influences product sense
Official's mass；Use pectase purpose like fibrous element enzyme；The use of the purpose of papain is that bed blending is micro- in degradable fermented liquid
Protein caused by biofermentation (macromolecular, need to could be absorbed, and the later stage easily high-temperature denatured flocculates through enteral digestion
Producing precipitation influences product sensory) it is small molecule amino acid (can be directly absorbed by the body for nutriment).
F, enzymolysis liquid obtained by step E is filtered to remove filter residue and maintains 5-10min to make enzyme sudden at 95-100 DEG C filtrate
Go out；Enzyme denaturation is inactivated under high temperature, prevents enzyme from excessively digesting；The enzyme being quenched includes cellulase, pectase and Papain
Enzyme；
G, bird's nest is ground into (0.5-1.5) cm × (0.5-1.5) cm chips, it is broken obtains bird's nest；Toward the bird's nest of 1 part of quality
The broken middle water for adding 20 parts of volumes, soaks 5-8h at 35-45 DEG C, is filtered to remove not by the moisture of bird's nest absorption and chooses removal of impurities
Matter；
H, into step G resulting materials, the water for the bird's nest 10 parts of volumes of broken middle addition for having absorbed moisture toward 1 part of quality, mix
Uniform, the addition broken dry weight 1wt%-3wt% papains of bird's nest are closed, 2-5h is digested at 45-55 DEG C；
I, 10wt%-15wt% sucrose is added into liquid obtained by step H, continuing 5-15min at 101-110 DEG C is gone out
Bacterium, after cooling according to active bacterium number be 1 × 109Cfu/g, inoculum concentration are 50-100g/t inoculating lactic acid bacteriums, in 28-34 DEG C of fermentation
1-2 months, obtain the zymotic fluid containing bird's nest；
J, gained liquid in step I is well mixed to obtain containing the broken white fungus bird's nest ferment of bird's nest with gained liquid in step F
Liquid；
K, 5wt%-10wt% FOSs are added into liquid obtained by step J, are well mixed；
The breaks down proteins in raw material (bird's nest) are not only able to pass through into small molecules such as amino acid using papain
Enzymolysis makes bird's nest become softer more smooth, the type and quantity of small molecule amino acid is added also by enzymolysis, after fermentation
Nutrition and fragranced are more prominent.
L, it is liquid obtained by step K is filling in carrying out, and duration 10min is sterilized at 101-110 DEG C, packaging,
It is made.
Embodiment one
One kind has antioxidation white fungus bird's nest enzyme liquid, and its detailed preparation method comprises the following steps：
A, auricle color and luster is selected in golden yellow, glossy, substantially light loose, fleshy hypertrophy, a tough and tensile and flexible silver
Ear 50g is ground into micro mist, crosses 50 mesh sieves and removes bulky grain, accurately weighs 40g micro mists and adds 1600mL distilled water immersions 1h；
B, by gains in step A, hot dipping carries 0.5h under 110 DEG C, 0.105MPa HTHPs, and boiling is mended during extracting
Add appropriate distilled water, mended after extraction and be filled with water to original volume；
C, fresh Opuntia ficus' fruit deburring remove seed is selected, accurately weighs the fresh Opuntia ficus' fruits of 20g, is added
100mL distilled water is polished into homogenate, and polishing forms the homogenate of Opuntia ficus' fruit, mixed with the leaching liquid of Tremella fuciformims Berk in step B
It is even, obtain white fungus-Opuntia ficus' fruit mixed liquor；
D, 200g sucrose is added into liquid obtained by step C, 110 DEG C of autoclave sterilization 15min, is subsequently cooled to room temperature
It is afterwards 1 × 10 by 100g/t inoculum concentration inoculating active bacterium number9Cfu/g active microbe powder, is stirred and evenly mixed, sealing, 34 DEG C of conditions
Lower fermentation 2 months；
E, 0.3g cellulase, 0.3g pectases and 0.3g papains is added into zymotic fluid obtained by step D, 50
2h is digested at DEG C；
F, enzymolysis after fermentation liquid obtained by step E is crossed into 200 mesh screens and removes filter residue, filtrate is heated to 95 DEG C and continued
10min, the cellulase, pectase and papain for making to add in step E inactivate, enzymolysis reaction；
G, the bird's nest 40g that swallow silk is fine and closely woven, small cup type is big and body is thick is selected, it is broken to be ground into 0.5cm × 0.5cm bird's nests, accurate to claim
Take 30g bird's nests broken, add 600mL distilled water and soak 5h at 45 DEG C, be filtered to remove not by the moisture of bird's nest absorption and choose removal of impurities
Matter, form 300g and be soaked the bird's nest of expansion；
H, be soaked the bird's nest of expansion toward 300g in step G, adds 3000mL distilled water, is well mixed, and adds 0.3g pawpaws
Protease, 5h is digested at 45 DEG C；
I, 330g sucrose is added into liquid obtained by step H, sterilize 15min at 101 DEG C, is subsequently cooled to after room temperature by 50g/
T inoculum concentration inoculating active bacterium number is 1 × 109Cfu/g active microbe powder, is stirred and evenly mixed, sealing, is fermented 1 under the conditions of 34 DEG C
Month；
J, gained liquid in gained liquid in step I and step F is well mixed, obtains the white fungus bird's nest fermentation containing bird's nest
Liquid；
K, 210g FOS is added into liquid obtained by step J, is well mixed；
L, liquid obtained by step K is filling in carrying out, sterilize 10min at 110 DEG C, packaging, zymotic fluid finished product is made.
Embodiment two
A, auricle color and luster is selected in golden yellow, glossy, substantially light loose, fleshy hypertrophy, a tough and tensile and flexible silver
Ear 70g is ground into micro mist, crosses 50 mesh sieves and removes bulky grain, accurately weighs 60g micro mists and adds 2400mL distilled water immersions 2h；
B, by gains in step A, hot dipping carries 1h under 121 DEG C, 0.105MPa, and boiling adds appropriate distillation during extracting
Water, mended after extraction and be filled with water to original volume；
C, fresh Opuntia ficus' fruit deburring remove seed is selected, accurately weighs 10g, addition 50mL distilled water is polished into even
Slurry, is well mixed with the leaching liquid of Tremella fuciformims Berk obtained in step B, obtains white fungus-Opuntia ficus' fruit mixed liquor；
D, add 250g sucrose into liquid obtained by step C, 121 DEG C of high-temperature sterilization 10min, be subsequently cooled to after room temperature by
100g/t inoculum concentration inoculating active bacterium number is 1 × 109Cfu/g active microbe powder, is stirred and evenly mixed, and sealing, 28 DEG C of conditions issue
Ferment 2 months；
E, addition 0.7g cellulases, 0.7g pectases and 0.7g papains into zymotic fluid obtained by step D, 55 DEG C
Lower enzymolysis 2h；
F, enzymolysis after fermentation liquid obtained by step E is crossed into 200 mesh screens and removes filter residue, filtrate is heated to 100 DEG C and continued
2min, the cellulase, pectase and papain for making to add in step E inactivate, enzymolysis reaction：
G, the bird's nest 20g that swallow silk is fine and closely woven, small cup type is big and body is thick is selected, it is broken to be ground into 1 × 1cm, and it is broken accurately to weigh 10g bird's nests
Bits, add 200mL distilled water and soak 8h at 35 DEG C, be filtered to remove not by the moisture of bird's nest absorption and choose the removal of impurity, formed
100g is soaked the bird's nest of expansion；
H, 1000mL distilled water is added toward the be soaked bird's nests of expansion of 100g in step G, is well mixed, adds 0.3g pawpaw
Protease, 2h is digested at 55 DEG C；；
I, 110g sucrose is added into liquid obtained by step H, sterilize 5min at 110 DEG C, is subsequently cooled to after room temperature by 50g/t's
Inoculum concentration inoculating active bacterium number is 1 × 109Cfu/g active microbe powder, is stirred and evenly mixed, sealing, is fermented 1 month under the conditions of 34 DEG C；
J, the well mixed white fungus bird's nest hair obtained containing bird's nest of gained liquid in liquid step F obtained by step I is accurately weighed
Zymotic fluid.
K, 420g FOSs are added into liquid obtained by step J, are well mixed；
N, liquid obtained by step M is filling in carrying out, sterilize 10min at 110 DEG C, packaging, zymotic fluid finished product is made.
Embodiment three
A, auricle color and luster is selected in golden yellow, glossy, substantially light loose, fleshy hypertrophy, a tough and tensile and flexible silver
Ear 60g is ground into micro mist, crosses 50 mesh sieves and removes bulky grain, accurately weighs 50g micro mists and adds 2000mL distilled water immersions 1.5h；
B, by gains in step A, hot dipping carries 0.8h under 115 DEG C, 0.08MPa, and boiling adds appropriate distillation during extracting
Water, mended after extraction and be filled with water to original volume；
C, select fresh Opuntia ficus' fruit deburring to remove son, accurately weigh the fresh Opuntia ficus' fruits of 15g, add
75mL distilled water is polished into homogenate, and polishing forms the homogenate of Opuntia ficus' fruit, is well mixed with leaching liquid of Tremella fuciformims Berk in step B,
Obtain white fungus-Opuntia ficus' fruit mixed liquor；
D, add 320g sucrose into liquid obtained by step C, 115 DEG C of high-temperature sterilization 12min, be subsequently cooled to after room temperature by
50g/t inoculum concentration inoculating active bacterium number is 1 × 109Cfu/g active microbe powder, is stirred and evenly mixed, sealing, is fermented under the conditions of 34 DEG C
2 months；
E, 0.5g cellulase, 0.5g pectases and 0.5g papains is added into zymotic fluid obtained by step D, 50
5h is digested at DEG C；
F, enzymolysis after fermentation liquid obtained by step E is crossed into 200 mesh screens and removes filter residue, filtrate is heated to 98 DEG C and continued
8min, the cellulase, pectase and papain for making to add in step E inactivate, enzymolysis reaction：
G, the bird's nest 30g that swallow silk is fine and closely woven, small cup type is big and body is thick is selected, it is broken to be ground into 1cm × 1cm bird's nests, accurately weighs 20g
Bird's nest is broken, adds 400mL distilled water and soaks 5h at 45 DEG C, is filtered to remove not by the moisture of bird's nest absorption and chooses the removal of impurity, shape
Be soaked the bird's nest of expansion into 200g；；
H, toward 200g in step G be soaked expansion bird's nest add 2000mL distilled water, be well mixed, add bird's nest dry weight
0.6g papain, 3h is digested at 50 DEG C..
I, 220g sucrose is added into liquid obtained by step H, sterilize 10min at 105 DEG C, is subsequently cooled to after room temperature by 50g/t
Inoculum concentration inoculating active bacterium number be 1 × 109Cfu/g active microbe powder, is stirred and evenly mixed, sealing, is fermented 1 under the conditions of 34 DEG C
Month.
J, gained liquid in gained liquid in step I and step F is well mixed and obtains the white fungus bird's nest zymotic fluid containing bird's nest；
K, the FOS of the 515g of liquid quality obtained by step L is added into liquid obtained by step J, is well mixed.
N, liquid obtained by step M is filling in carrying out, sterilize 10min at 105 DEG C, packaging, zymotic fluid finished product is made.
The embodiment of the present invention three is obtained with the progress zoopery of antioxidation white fungus bird's nest enzyme liquid, detect it
Antioxidant effect, concretely comprise the following steps：
(1) from the ICR mouse that body weight is (20 ± 2) g, male and female half and half, adapt to raising observation in one week and have no adverse reaction.
(2) healthy ICR mouse are randomly divided into normal group, positive controls, D- galactolipins model control group and ferment to do
Pre- group, every group 10, male and female have.In addition to normal group and positive controls feeding standard feed, D- galactolipin model control groups
100mg/ (Kgbw) D- galactolipin normal saline solutions are subcutaneously injected in neck with the daily timing of ferment intervention group mouse, normally
Group and positive controls injection 0.05mL/10g sterile salines.
(3) positive controls and the daily gavage white fungus bird's nest enzyme liquid 1mL/100g of ferment intervention group from modeling first day,
Gavage gives respective volume physiological saline respectively daily for normal group and D- galactolipin model control groups mouse.
(4) terminate fasted for one day prior in experiment, blood sampling, 3500r/min centrifugation 10min, separate serum and use kit
SOD, CAT, GSH-Px, MDA and T-AOC content in mice serum are determined, is shown in Table
1.The each group of table 1 influences on mouse antioxidation activity
| SOD(U/mL) | CAT(U/mL) | GSH-Px(U/mL) | MDA(nmol/mgprot) | T-AOC(U/mL) | |
| Normal group | 96.3±1.17 | 2.45±0.87 | 134.8±2.43 | 5.81±0.13 | 6.36±0.11 |
| Positive controls | 103.1±0.96 | 2.66±0.51 | 143.7±1.09 | 5.01±0.09 | 7.25±0.18 |
| D- galactolipin model group model groups | 73.6±1.37 | 1.18±0.10 | 83.6±2.86 | 9.65±0.58 | 4.03±0.13 |
| Ferment intervention group | 83.7±1.40 | 1.65±0.07 | 93.4±1.39 | 7.66±0.13 | 4.72±0.06 |
As shown in Table 1 in ferment intervention group mice serum SOD, CAT, GSH-Px, T-AOC content apparently higher than D- galactolipins
Model group, MAD contents are substantially relatively low compared with D- galactolipins model group；SOD, CAT, GSH-Px, T- in positive controls mice serum
AOC contents are apparently higher than normal group, and MAD contents are substantially relatively low compared with normal group；Result above shows white fungus prepared by the present invention
Bird's nest enzyme liquid has the function that very strong for enhancing mouse inoxidizability.Therefore, the daily drunk ferment is for improving body
Firm oxidisability, anti-aging, which has, greatly to help.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, either which part or all technical characteristic are entered
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme.
Claims (3)
1. one kind has antioxidation white fungus bird's nest enzyme liquid, its spy is just, includes the component of following parts by weight：White fungus
40-60 parts, bird's nest 10-30 parts and Opuntia ficus' fruit 10-20 parts.
2. according to claim 1 have antioxidation white fungus bird's nest enzyme liquid, it is characterised in that including following weight
Part：15 parts of 50 parts of white fungus, 20 parts of bird's nest and Opuntia ficus' fruit.
3. a kind of preparation method as described in claim 1 or 2 with inoxidizability effect white fungus bird's nest enzyme liquid, it is special
Sign is, comprises the following steps：
A, white fungus is ground into Tremella micropowder, the Tremella micropowder toward 1 part of quality adds the water of 40 parts of volumes, soaks 1-2h, obtains
White fungus soak；
B, the white fungus soak obtained in step A is placed in cooker, at 110-121 DEG C, 0.045-0.105MPa is extracted
0.5-1h, obtain leaching liquid of Tremella fuciformims Berk；
C, by fresh Opuntia ficus' fruit deburring remove seed, fresh Opuntia ficus' fruit toward 1 part of quality adds 5 parts of volumes
Water, the homogenate of Opuntia ficus' fruit is polished into, is mixed with the step B leaching liquid of Tremella fuciformims Berk obtained, obtain white fungus-Opuntia ficus fruit
Real mixed liquor；
D, the white fungus obtained toward step C-Opuntia ficus' fruit mixed liquor adds 10wt%-20wt% sucrose, and 110-121 DEG C goes out
Bacterium cooling after according to active bacterium number be 1 × 109Cfu/g, inoculum concentration are 50-100g/t inoculating lactic acid bacteriums, in 28-34 DEG C of fermentation 2
Individual month；
E, white fungus dry weight and Opuntia ficus' fruit dry weight ratio contained by accounting for are added in step D into liquid obtained by step D is
The papain of 0.5wt%-1wt% cellulase, 0.5wt%-1wt% pectase and 0.5wt%-1wt%, in
2-5h is digested at a temperature of 45-55 DEG C；
F, enzymolysis liquid obtained by step E is filtered to remove filter residue and maintains 5-10min to be quenched enzyme at 95-100 DEG C filtrate；
G, bird's nest is ground into (0.5-1.5) cm × (0.5-1.5) cm chips, it is broken obtains bird's nest；Toward 1 part of quality bird's nest it is broken in
The water of 20 parts of volumes is added, soaks 5-8h at 35-45 DEG C, is filtered to remove not by the moisture of bird's nest absorption and chooses the removal of impurity；
H, in step G resulting materials, the water for the bird's nest 10 parts of volumes of broken middle addition for having absorbed moisture toward 1 part of quality, it is well mixed,
The broken dry weight 1wt%-3wt% papains of bird's nest are added, 2-5h is digested at 45-55 DEG C；
I, 10wt%-15wt% sucrose is added into liquid obtained by step H, continuing 5-15min at 101-110 DEG C is sterilized, cold
But after according to active bacterium number be 1 × 109Cfu/g, inoculum concentration are 50-100g/t inoculating lactic acid bacteriums, are fermented in 28-34 DEG C 1-2
Month, obtain the zymotic fluid containing bird's nest；
J, gained liquid in step I is well mixed to obtain containing the broken white fungus bird's nest enzyme liquid of bird's nest with gained liquid in step F；
K, 5wt%-10wt% FOSs are added into liquid obtained by step J, are well mixed；
L, it is liquid obtained by step K is filling in carrying out, and duration 10min is sterilized at 101-110 DEG C, is packed, and is made.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108324662A (en) * | 2018-03-09 | 2018-07-27 | 西安惠可欣微生物科技有限公司 | A kind of novel food materials ferment skin-beautifying product and preparation method thereof |
| CN108371324A (en) * | 2018-01-18 | 2018-08-07 | 浙江燕宝丽堂电子商务有限公司 | A kind of preparation process of bird's nest essence oral solution |
| CN108893511A (en) * | 2018-06-28 | 2018-11-27 | 燕生（福建）生物工程有限公司 | A kind of method that bird's nest extracts small-molecular peptides |
| CN111387489A (en) * | 2020-03-27 | 2020-07-10 | 益生活力健康科技(厦门)有限公司 | Lactobacillus bird's nest product and preparation method thereof |
| CN111418827A (en) * | 2020-03-27 | 2020-07-17 | 益生活力健康科技(厦门)有限公司 | Application of lactobacillus plantarum in improvement of bird's nest enzymolysis fermentation flavor and increase of small molecular peptides and sialic acid |
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103519171A (en) * | 2013-10-17 | 2014-01-22 | 天津市星河系科技有限公司 | Preparation method of cactus enzyme |
| CN103549385A (en) * | 2013-10-17 | 2014-02-05 | 天津市星河系科技有限公司 | Preparation method of agaric ferment |
| CN104000176A (en) * | 2014-04-28 | 2014-08-27 | 吉林华鑫菌业有限责任公司 | Preparation method for auricularia auricula ferment |
| CN105166915A (en) * | 2015-10-29 | 2015-12-23 | 苏剑锋 | Preparation method for cactus and taxus chinensis enzyme |
| CN105249090A (en) * | 2015-09-09 | 2016-01-20 | 安徽管仲宫神生物科技有限公司 | Anti-wrinkle beauty-maintaining enzyme drink and preparation method therefor |
| CN105395468A (en) * | 2015-12-25 | 2016-03-16 | 北京工商大学 | Cactus fermented raw stock and preparation method and application thereof |
| CN105769739A (en) * | 2016-04-29 | 2016-07-20 | 厦门生之元生物科技有限公司 | Fructus Lycii fermentation liquor for delaying senescence and skin care products of Fructus Lycii fermentation liquor for delaying senescence |
| CN105768092A (en) * | 2016-04-08 | 2016-07-20 | 厦门元之道生物科技有限公司 | Comprehensive fermentation liquor with antibacterial activity and application thereof |
| CN105901716A (en) * | 2016-04-29 | 2016-08-31 | 厦门生之元生物科技有限公司 | Antioxidant enzyme with resveratrol and application of antioxidant enzyme |
| CN105942503A (en) * | 2016-05-09 | 2016-09-21 | 苏剑锋 | Preparation method of nycium and opuntia milpa alta ferment nutrition and ferment nutrition prepared by method |
| CN106262838A (en) * | 2016-08-18 | 2017-01-04 | 贵州庆福裕兴生物科技有限公司 | A kind of Fructus Rosae Normalis Hericium erinaceus (Bull. Ex Fr.) Pers. ferment and preparation method thereof |
| CN106722885A (en) * | 2017-01-16 | 2017-05-31 | 四川省大真科技有限责任公司 | A kind of fermented white fungus leaching liquor and preparation method thereof and the jelly for preparing |
-
2017
- 2017-09-14 CN CN201710828926.3A patent/CN107455748A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103519171A (en) * | 2013-10-17 | 2014-01-22 | 天津市星河系科技有限公司 | Preparation method of cactus enzyme |
| CN103549385A (en) * | 2013-10-17 | 2014-02-05 | 天津市星河系科技有限公司 | Preparation method of agaric ferment |
| CN104000176A (en) * | 2014-04-28 | 2014-08-27 | 吉林华鑫菌业有限责任公司 | Preparation method for auricularia auricula ferment |
| CN105249090A (en) * | 2015-09-09 | 2016-01-20 | 安徽管仲宫神生物科技有限公司 | Anti-wrinkle beauty-maintaining enzyme drink and preparation method therefor |
| CN105166915A (en) * | 2015-10-29 | 2015-12-23 | 苏剑锋 | Preparation method for cactus and taxus chinensis enzyme |
| CN105395468A (en) * | 2015-12-25 | 2016-03-16 | 北京工商大学 | Cactus fermented raw stock and preparation method and application thereof |
| CN105768092A (en) * | 2016-04-08 | 2016-07-20 | 厦门元之道生物科技有限公司 | Comprehensive fermentation liquor with antibacterial activity and application thereof |
| CN105769739A (en) * | 2016-04-29 | 2016-07-20 | 厦门生之元生物科技有限公司 | Fructus Lycii fermentation liquor for delaying senescence and skin care products of Fructus Lycii fermentation liquor for delaying senescence |
| CN105901716A (en) * | 2016-04-29 | 2016-08-31 | 厦门生之元生物科技有限公司 | Antioxidant enzyme with resveratrol and application of antioxidant enzyme |
| CN105942503A (en) * | 2016-05-09 | 2016-09-21 | 苏剑锋 | Preparation method of nycium and opuntia milpa alta ferment nutrition and ferment nutrition prepared by method |
| CN106262838A (en) * | 2016-08-18 | 2017-01-04 | 贵州庆福裕兴生物科技有限公司 | A kind of Fructus Rosae Normalis Hericium erinaceus (Bull. Ex Fr.) Pers. ferment and preparation method thereof |
| CN106722885A (en) * | 2017-01-16 | 2017-05-31 | 四川省大真科技有限责任公司 | A kind of fermented white fungus leaching liquor and preparation method thereof and the jelly for preparing |
Cited By (7)
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| CN108371324A (en) * | 2018-01-18 | 2018-08-07 | 浙江燕宝丽堂电子商务有限公司 | A kind of preparation process of bird's nest essence oral solution |
| CN108324662A (en) * | 2018-03-09 | 2018-07-27 | 西安惠可欣微生物科技有限公司 | A kind of novel food materials ferment skin-beautifying product and preparation method thereof |
| CN108893511A (en) * | 2018-06-28 | 2018-11-27 | 燕生（福建）生物工程有限公司 | A kind of method that bird's nest extracts small-molecular peptides |
| CN111387489A (en) * | 2020-03-27 | 2020-07-10 | 益生活力健康科技(厦门)有限公司 | Lactobacillus bird's nest product and preparation method thereof |
| CN111418827A (en) * | 2020-03-27 | 2020-07-17 | 益生活力健康科技(厦门)有限公司 | Application of lactobacillus plantarum in improvement of bird's nest enzymolysis fermentation flavor and increase of small molecular peptides and sialic acid |
| CN111418827B (en) * | 2020-03-27 | 2023-05-02 | 厦门和美科盛生物技术有限公司 | Lactobacillus plantarum with functions of improving enzymolysis and fermentation flavor of bird's nest and increasing small molecular peptides and sialic acid |
| CN112617180A (en) * | 2020-12-21 | 2021-04-09 | 重庆八零四零生物工程研究院有限公司 | Comprehensive cactus enzyme |
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