CN104087631A - Method for producing Antrodia camphorata extracellular polysaccharides by deep liquid fermentation - Google Patents
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Abstract
The invention discloses a method for producing Antrodia camphorata extracellular polysaccharides by deep liquid fermentation, particularly an industrial-scale production method of Antrodia camphorata extracellular polysaccharides. The method comprises the following steps: inoculating activated Antrodia camphorata strain into a seed tank to perform amplification culture, inoculating the liquid seed into a purpose-made polysaccharide-producing liquid culture medium to perform fermentation, separating the fermentation liquid, and extracting the extracellular polysaccharides. In order to provide the yield of the extracellular polysaccharides, the method optimizes the formula of the fermentation culture medium, and adopts the compound formula against the carbon source part, thereby ensuring the biomass of the earlier-stage thallus and also ensuring the yield of the later-stage metabolites. The method can be used for preparing high-quality polysaccharides with different contents, and the polysaccharide content can be 20-50%.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of method that camphor tree sesame exocellular polysaccharide is produced in deep layer liquid state fermentation.
Background technology
Camphor tree sesame has another name called the raw Antrodia of camphor tree, Cinnamomum kanahirai hay mushroom, Antrodia camphorata, red camphor tree sesame, camphor tree mushroom, camphor tree wild rice, wild rice in camphor tree cave, red camphor tree, is subordinate to Mycophyta, Basidiomycotina, Hymenomycetes, polyporaceae, Antrodia belongs to perennial Mushrooms, is peculiar a kind of rare, the undeveloped famous and precious pharmaceutical fungi and edible fungi still in Taiwan.Only be grown in the rotten heartwood inwall of distinctive Cinnamomum kanahirai hay tree trunk between 450～2000 meters of mountain area, Taiwan height above sea level, or the surface of the dark humidity of the Cinnamomum kanahirai hay timber of withered lodging, find really difficult.Camphor tree sesame is as the distinctive bacterial classification in Taiwan, and in physical environment, Cinnamomum kanahirai hay tree is its unique host, and general fungi can not grow thereon.Because camphor tree sesame can cause Cinnamomum kanahirai hay tree cavity, center rotten, add that people sets excessive and random lumbering to Cinnamomum kanahirai hay, cause Cinnamomum kanahirai hay tree quantity quite rare, be listed at present one-level child care seeds.In the last hundred years, Cinnamomum kanahirai hay tree is rare to see, can grow the Cinnamomum kanahirai hay tree of Antrodia camphorata still less, and its result causes wild camphor tree sesame resource critical shortage.Therefore camphor tree sesame is very precious, is called as " refreshing sesame " in Hong Kong, Macao and Taiwan, and Taiwan is among the people claims camphor tree sesame for " ruby in forest ".
In recent years, research learns that camphor tree sesame can effectively treat hepatopathy and other side as antitumor, antiviral, antianaphylaxis, reducing blood-fat, hypoglycemic disease.In camphor tree sesame, contain multiple physiologically active ingredient, as polysaccharide, triterpene compound, sudismase (SOD), adenosine, protein (containing immune protein), multivitamin, trace element (calcium, phosphorus), nucleic acid, lectin, amino acid, cholesterol, xylogen, blood pressure stabilization material etc.Research is found, camphor tree sesame polysaccharide is the main active substances in camphor tree sesame, its content in camphor tree sesame fermented supernatant fluid is up to 3%, there is the physiologically actives such as immune physiologically active, prevention and control tumour, main Han β ?D ?glucan (β ?D ?dextran)) its effect is to see through to excite scavenger cell, T lymphocyte, bone-marrow-derived lymphocyte and natural killer cell etc. to strengthen immunologic function, and then reaches antineoplastic effect.In addition camphor tree sesame polysaccharide also has the effects such as cardiac stimulant, hypotensive, hypoglycemic, antithrombotic.
To the research of camphor tree sesame, be not also very thorough both at home and abroad at present, the main culture method of taking is solid fermentation method.Because this method fermentation period is long, labour intensity is large, and floor space is wide, is subject to seasonal restrictions, and easily pollutes, and should not carry out the shortcomings such as pure-blood ferment, causes camphor tree sesame fermentation scale to can not get expanding always.For this reason, with biotechnological means, carry out natural camphor tree sesame liquid submerged fermentation cultivation and can shorten incubation time, obtain a large amount of and pure mycelium and polysaccharide, cost is low, can realize serialization, the automatization of fermentation industry, promote productivity effect, become the approach of very worth exploitation.Also there is the investigator of a small amount of universities and colleges to carry out research to the submerged fermentation of camphor tree sesame both at home and abroad; but often its main special emphasis is how to obtain higher biomass; only have small part investigator to pay close attention to the research of the functional component polysaccharide of camphor tree sesame; but be often all only confined to the shake flask test of lab scale; belong to phase of basic research; and the outer sugared yield of its biomass and active result born of the same parents is lower; production cost is too expensive; its technique needs further to promote, if will reach the production camphor tree sesame polysaccharide of mass-producing and industrialization, also needs to carry out a large amount of research and cut-and-try work.
Summary of the invention
The object of the invention is in order to solve the technical bottleneck of commercial scale production camphor tree sesame polysaccharide, adopt liquid state fermentation to produce camphor tree sesame polysaccharide and overcome that the solid state fermentation cycle is long, investment is large, be subject to seasonal restrictions, be difficult to the drawbacks such as industrialization, adopt this invention can be continuously, automatization, industrialization produce high-quality camphor tree sesame polysaccharide.
Object of the present invention is achieved through the following technical solutions:
A method of preparing camphor tree sesame fermented liquid, the method comprises the steps:
Liquid fermentation medium after camphor tree sesame seed culture fluid access sterilizing is carried out to deep layer liquid state fermentation and obtain camphor tree sesame fermentation culture; Described deep layer liquid state fermentation inoculum size is 10～20% (g/100g, lower with), ventilating ratio be 1:0.3 ?1.0vvm, tank pressure 0.02～0.05Mpa cultivates 4～7 days at 22～30 ℃; Described liquid fermentation medium is: the high temperature resistant α-amylase 0.001-0.007% (v/v) of glucose 1-3%, starch 1-3%, peptone 0.1-0.5%, wheat bran 0.1-0.5%, potassium primary phosphate 0.1-0.5%, magnesium sulfate heptahydrate 0.1-0.5%, defoamer 0.01-0.05%, 20000u/ml, regulates pH value 4.8.During above-mentioned substratum forms, except high-temperatureα-amylase, remaining " % " all represents g/100ml.
Described defoamer can be the materials such as vegetables oil (soya-bean oil, rapeseed oil) class, organic ethers.
Wherein said camphor tree sesame seed culture fluid is preferably cultivated acquisition by secondary, and concrete grammar is as follows:
1) first order seed is cultivated: by the liquid seed culture medium after activated shaking flask bacterial classification access sterilizing, inoculum size is 0.1～2%, ventilating ratio be 1:0.3 ?1.0vvm, tank pressure 0.02～0.05Mpa, at 22～30 ℃, cultivate 6～8 days, obtain camphor tree sesame first order seed nutrient solution, standby;
2) secondary seed cultivation: by the step 1) liquid seed culture medium after the first order seed nutrient solution access sterilizing of gained, inoculum size is 10～20%, ventilating ratio be 1:0.3 ?1.0vvm, tank pressure 0.02～0.05Mpa, at 22～30 ℃, cultivate 4～7 days, obtain described camphor tree sesame seed culture fluid.
Described liquid seed culture medium is preferably: glucose 2-4%, peptone 0.1-0.5%, yeast powder 1-3%, potassium primary phosphate 0.1-0.5%, magnesium sulfate heptahydrate 0.1-0.5%, defoamer 0.01-0.05%, regulate pH value 4.8.The volume of first class seed pot is 100-500L, and the volume of secondary seed tank is 1000-5000L, and loadings is 70-90%." % " during above-mentioned substratum forms all represents g/100ml.
Fermented liquid is cultivated endpoint standard: fermented liquid gives off a strong fragrance, bacterium ball becomes broken, filtrate is muddy; PH value, reducing sugar are down to minimum and slightly fluctuation, and pH value 3.0-4.0, below reducing sugar 1.0% (g/100g); Microscopy mycelia is in small, broken bits, and a large amount of spores, without living contaminants.
The method that camphor tree sesame exocellular polysaccharide extracts, comprises the steps:
1) separation: the camphor tree sesame separation of fermentative broth by obtaining according to the method described above, obtains camphor tree sesame filtrate;
2) concentrated: by step 1) filtrate of gained adopts secondary cryoconcentration, and obtaining concentrated proportion is the thickened pulp of 1.25～1.40 (50 ℃ of heat are surveyed).
3) alcohol precipitation, oven dry: 95% alcohol that adds thickened pulp weight 3-5 doubly to measure, with Ebullioscope, detect the about 65-80% of mixed solution alcoholic strength (v/v), standing 8-16h, carries out vacuum drying by throw out, temperature 50-75 ℃, vacuum tightness is not less than 0.085MPa; Drying to being dried thing moisture content is 3～8% (g/100g), obtains the outer sugar of born of the same parents.
Described separation preferably adopts horizontal screw centrifuge to carry out, rotating speed be controlled at 2500 ?3500r/min.
Step 2) the secondary simmer down to adopting: one-level adopt 1 ?3t double-effect evaporator concentrated, by fermented liquid be concentrated to proportion be 1.15 ?1.20 (50 ℃ heat survey), secondary adopt 1 ?3t haplo-effect concentrator concentrated, be about to proportion and be 1.1 ?the concentrated solution of 1.20 (50 ℃ of heat are surveyed) continue to be concentrated to the thickened pulp that proportion is 1.25～1.40 (50 ℃ of heat are surveyed); Concentration process all requires to control temperature not higher than 75 ℃, and vacuum tightness is not less than 0.085MPa.
In the present invention alcohol precipitation be adopt 1 ?3t Alcohol-settling tank, alcohol precipitation number of times is more, the content of the outer sugar of born of the same parents is higher.The vacuum drying oven that vacuum drying adopts, the outer sugared content of born of the same parents that the present invention extracts 20 ?50%.
The present invention has advantages of following outstanding:
For being amplified to by lab scale, the technical barrier that runs in suitability for industrialized production studies just in the present invention, at lab scale and pilot scale, continue to optimize fermention medium (especially carbon source), promote on the basis of biomass and exocellular polysaccharide, the difficult problems such as the Fermentation Process of Parameter control that runs in amplification process, extraction equipment type selecting, optimization have been solved, final successful grafting to 10 ?20t fermentor tank carry out industrialization production, and produce smoothly high-quality camphor tree sesame polysaccharide, really realized continuously, the production of automatization, industrialization.
In order to improve the output of object product exocellular polysaccharide, the present invention is optimized and combines in the carbon source of fermention medium: the compound prescription that adopts fugitive glucose and long-acting starch.Earlier fermentation is with growth mycelium, and it is main improving biological content, so in substratum, has added the glucose carbon source that thalline is preferentially selected, rapidly propagation; The fermentation later stage will be improved the content of object product, so carbon source provides thalline relatively to utilize slower starch, and in substratum, adds appropriate amount of starch enzyme, controls thalline quantity in suitable scale, the outer sugar of the raw metabolite born of the same parents of not stopping pregnancy.
For born of the same parents are provided the output of outer sugar, the present invention optimizes the formula of fermention medium, for carbon source, has partly adopted compound prescription, has both guaranteed the biomass of thalline in early stage, guarantees again the output of later stage meta-bolites.
Adopt the present invention to make the high-quality polysaccharide of different content according to the market requirement and product standard, polysaccharide content can 20 ?50%.
Embodiment
By specific embodiment, further illustrate the present invention below.But the detail of embodiment only, for explaining the present invention, should not be construed as limited overall technical solution.
The camphor tree sesame bacterial strain that following examples are used is bought in institute of microbiology of Chinese Academy of Sciences common micro-organisms center, bacterial classification coding CGMCC NO.0543, and take this strain bacterium is example, describes concrete scheme of the present invention in detail.But the inventive method is common to all crow spirit ginseng bacterium, is not limited in this bacterial strain.
Embodiment 1
Industrialization deep layer liquid state fermentation of the present invention is produced the method for camphor tree sesame exocellular polysaccharide and is carried out in the steps below:
1, the preparation of camphor tree sesame fermented liquid: the camphor tree sesame shaking flask bacterial strain having activated is inoculated in to seed culture medium (300L) by inoculum size 1%, wherein seed culture medium consists of glucose 3%, peptone 0.2%, yeast powder 1%, potassium primary phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, with dilute sulphuric acid, regulate pH value 4.8, ventilating ratio is 1:0.3vvm, tank pressure 0.03Mpa, 26～28 ℃ of shaking culture obtain camphor tree sesame primary seed solution for 7 days; Primary seed solution is accessed to secondary seed medium (3000L) by inoculum size 15%, wherein secondary seed medium consists of glucose 3%, peptone 0.2%, yeast powder 1%, potassium primary phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, with dilute sulphuric acid, regulate pH value 4.8, ventilating ratio is 1:0.3vvm, tank pressure 0.03Mpa, cultivates for 26～28 ℃ and within 5 days, obtains camphor tree sesame secondary seed solution; Secondary seed solution is accessed to fermention medium (7500L) by inoculum size 18%, wherein fermention medium consists of glucose 2%, starch 2%, peptone 0.3%, wheat bran 0.3%, potassium primary phosphate 0.2%, magnesium sulfate heptahydrate 0.2%, soya-bean oil 0.03%, Nai Gaowen α ?amylase (Zaozhuang biological company limited of outstanding promise enzyme, 20000u/ml) 0.002% (v/v), regulates pH value 4.8 with dilute sulphuric acid; Ventilating ratio is 1:0.5vvm, and tank pressure 0.03Mpa cultivates 7 days for 26～28 ℃, ferment to fermented liquid give off a strong fragrance (milk fragrant smell), bacterium ball becomes broken, filtrate is muddy; PH value is down to minimum 3.2 also slightly fluctuations, and reducing sugar is down to minimum 0.6 also slightly fluctuation; Microscopy mycelia is in small, broken bits, and a large amount of spores, without living contaminants, obtain camphor tree sesame fermented liquid 8850Kg.
2, camphor tree sesame separation of fermentative broth: above-mentioned camphor tree sesame fermented liquid 8850Kg is carried out to separation by horizontal screw centrifuge, obtain the wet mycelium (water content is 75%) of camphor tree sesame filtrate 8400Kg and 350Kg, wet mycelium directly cryodrying pulverizing makes fermentation camphor tree sesame bacterium powder.Concrete technology condition is: rotating speed is controlled at 3000r/min, and disengaging time is at 2h.
4, cryoconcentration: by the camphor tree sesame filtrate 8400Kg vacuum transfer of step 2 gained to 3t double-effect evaporator, in vacuum tightness, be 0.085MPa, thickening temperature is under 65 ℃ of conditions, to be concentrated into proportion (the 50 ℃ of heat are surveyed) concentrated solution that is 1.20, again by concentrated solution vacuum transfer to 1.5t single-action ball-type thickener, in vacuum tightness, be 0.09MPa, thickening temperature is under 60 ℃ of conditions, to continue to be concentrated into the thickened pulp 300kg that proportion is 1.30 (50 ℃ of heat are surveyed).
5, alcohol precipitation, oven dry: to filling 95% alcohol that adds weight 1000kg in the Alcohol-settling tank of 300kg thickened pulp, with Ebullioscope, detect mixed solution alcoholic strength 72% (v/v), standing 12h, dries the separation of bottom settlings thing with vacuum drying oven, temperature 60 C, vacuum tightness 0.09MPa; Dry 15h and obtain the outer sugared 75kg of born of the same parents.Detecting moisture content is 5%, and polysaccharide content is 32%.
Embodiment 2
1, the preparation of camphor tree sesame fermented liquid: the camphor tree sesame shaking flask bacterial strain having activated is inoculated in to seed culture medium (300L) by inoculum size 1.5%, wherein seed culture medium consists of glucose 3%, peptone 0.2%, yeast powder 1%, potassium primary phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, with dilute sulphuric acid, regulate pH value 4.8, ventilating ratio is 1:0.3vvm, tank pressure 0.03Mpa, 26～28 ℃ of shaking culture obtain camphor tree sesame primary seed solution for 8 days; Primary seed solution is accessed to secondary seed medium (3000L) by inoculum size 15%, wherein secondary seed medium consists of glucose 3%, peptone 0.2%, yeast powder 1%, potassium primary phosphate 0.3%, magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, with dilute sulphuric acid, regulate pH value 4.8, ventilating ratio is 1:0.3vvm, tank pressure 0.03Mpa, cultivates for 26～28 ℃ and within 6 days, obtains camphor tree sesame secondary seed solution; Secondary seed solution is accessed to fermention medium (15000L) by inoculum size 18%, wherein fermention medium consists of glucose 2%, starch 2%, peptone 0.3%, wheat bran 0.3%, potassium primary phosphate 0.2%, magnesium sulfate heptahydrate 0.2%, rapeseed oil 0.03%, Nai Gaowen α ?amylase (Zaozhuang biological company limited of outstanding promise enzyme, 20000u/ml) 0.002% (v/v), regulates pH value 4.8 with dilute sulphuric acid; Ventilating ratio is 1:0.5vvm, and tank pressure 0.04Mpa cultivates 7 days for 26～28 ℃, ferment to fermented liquid give off a strong fragrance (having obvious milk fragrant smell), bacterium ball becomes broken, filtrate is muddy; PH value is down to minimum 3.0 also slightly fluctuations, and reducing sugar is down to minimum 0.4 also slightly fluctuation; Microscopy mycelia is in small, broken bits, and a large amount of spores, without living contaminants, had both obtained camphor tree sesame fermented liquid 17700Kg.
2, the separation of camphor tree sesame fermented liquid: above-mentioned camphor tree sesame fermented liquid 17700Kg is carried out to separation by horizontal screw centrifuge, obtain the wet mycelium (water content is 78%) of camphor tree sesame filtrate 16750Kg and 800Kg, wet mycelium directly cryodrying pulverizing makes fermentation camphor tree sesame bacterium powder.Concrete technology condition is: rotating speed is controlled at 3200r/min, and disengaging time is at 4.5h.
4, cryoconcentration: by the camphor tree sesame filtrate 16750Kg vacuum transfer of step 2 gained to 3t double-effect evaporator, in vacuum tightness, be 0.09MPa, thickening temperature is under 63 ℃ of conditions, to be concentrated into proportion (the 50 ℃ of heat are surveyed) concentrated solution that is 1.18, again by concentrated solution vacuum transfer to 1.5t single-action ball-type thickener, in vacuum tightness, be 0.09MPa, thickening temperature is under 65 ℃ of conditions, to be concentrated into the thickened pulp 550kg that proportion is 1.35 (50 ℃ of heat are surveyed).
5, alcohol precipitation, oven dry: to filling 95% alcohol that adds weight 2200kg in the Alcohol-settling tank of 550kg thickened pulp, with Ebullioscope, detect mixed solution alcoholic strength 70% (v/v), standing 10h, dries the separation of bottom settlings thing with vacuum drying oven, 65 ℃ of temperature, vacuum tightness-0.09MPa; Dry 18h and obtain the outer sugared 132kg of born of the same parents.Detecting moisture content is 4%, and polysaccharide content is 35%.
Embodiment 3
For the compound prescription of fugitive glucose and long-acting starch in better checking the present invention, in industrialization, produce the superiority of camphor tree sesame polysaccharide, done again several simultaneous tests.In test, only adjusted carbon source glucose in liquid fermentation medium or (with) consumption of starch, in comparative example 1, do not contain starch, glucose concn is 2.0%; In comparative example 2, do not contain glucose, starch concentration is 2.5%, and in comparative example 3, glucose content is 2.5%, and starch content is 0.5%; All the other conditions are all with the embodiment of the present invention 1, and test-results is in Table 1:
Table 1
Claims (7)
1. a method of preparing camphor tree sesame fermented liquid, is characterized in that the method comprises the steps:
Liquid fermentation medium after camphor tree sesame seed culture fluid access sterilizing is carried out to deep layer liquid state fermentation and obtain camphor tree sesame fermentation culture; Described deep layer liquid state fermentation inoculum size is 10～20% (g/100g), and ventilating ratio is 1:0.3-1.0vvm, and tank pressure 0.02～0.05Mpa cultivates 4～7 days at 22～30 ℃; Described liquid fermentation medium is: the high temperature resistant α-amylase 0.001-0.007% (v/v) of glucose 1-3%, starch 1-3%, peptone 0.1-0.5%, wheat bran 0.1-0.5%, potassium primary phosphate 0.1-0.5%, magnesium sulfate heptahydrate 0.1-0.5%, defoamer 0.01-0.05%, 20000u/ml, regulates pH value 4.8.
2. the method for preparing camphor tree sesame fermented liquid according to claim 1, is characterized in that described camphor tree sesame seed culture fluid cultivates acquisition by secondary, and concrete grammar is as follows:
1) first order seed is cultivated: by the liquid seed culture medium after activated shaking flask bacterial classification access sterilizing, inoculum size is 0.1～2%, ventilating ratio be 1:0.3 ?1.0vvm, tank pressure 0.02～0.05Mpa, at 22～30 ℃, cultivate 6～8 days, obtain camphor tree sesame first order seed nutrient solution, standby;
2) secondary seed cultivation: by the step 1) liquid seed culture medium after the first order seed nutrient solution access sterilizing of gained, inoculum size is 10～20%, ventilating ratio be 1:0.3 ?1.0vvm, tank pressure 0.02～0.05Mpa, at 22～30 ℃, cultivate 4～7 days, obtain described camphor tree sesame seed culture fluid.
3. a kind of camphor tree sesame fermented liquid method of preparing according to claim 2, it is characterized in that described liquid seed culture medium is: glucose 2-4%, peptone 0.1-0.5%, yeast powder 1-3%, potassium primary phosphate 0.1-0.5%, magnesium sulfate heptahydrate 0.1-0.5%, defoamer 0.01-0.05%, regulate pH value 4.8.
4. a kind of camphor tree sesame fermented liquid method of preparing according to claim 1, is characterized in that the cultivation terminal of deep layer liquid state fermentation: fermented liquid gives off a strong fragrance, bacterium ball becomes broken, filtrate is muddy; PH value, reducing sugar are down to minimum and slightly fluctuation, and pH value 3.0-4.0, below reducing sugar 1.0% (g/100g); Microscopy mycelia is in small, broken bits, and a large amount of spores, without living contaminants.
5. the method that camphor tree sesame exocellular polysaccharide extracts, is characterized in that the method comprises the steps:
1) separation: the camphor tree sesame fermented liquid of claim 1 gained is carried out to separation, obtain camphor tree sesame supernatant liquor;
2) concentrated: by step 1) filtrate of gained adopts secondary cryoconcentration, obtains the thickened pulp that concentrated proportion is 1.25～1.40;
3) alcohol precipitation, oven dry: 95% alcohol that adds thickened pulp weight 3-5 doubly to measure, with Ebullioscope, detect the about 65-80% of mixed solution alcoholic strength, standing 8-16h, carries out vacuum drying by throw out, temperature 50-75 ℃, vacuum tightness is not less than 0.085MPa; Drying to being dried thing moisture content is 3～8% (g/100g), obtains the outer sugar of born of the same parents.
6. the method that camphor tree sesame exocellular polysaccharide according to claim 5 extracts, is characterized in that described step 1) described in separation adopt horizontal screw centrifuge to carry out, rotating speed be controlled at 2500 ?3500r/min.
7. the method that camphor tree sesame exocellular polysaccharide according to claim 5 extracts, it is characterized in that described step 2) in the secondary simmer down to that adopts: one-level adopts double-effect evaporator concentrated, by fermented liquid be concentrated to proportion be 1.15 ?1.20, secondary adopts haplo-effect concentrator concentrated, be about to proportion and be 1.1 ?1.20 concentrated solution to continue to be concentrated to proportion be 1.25～1.40 thickened pulp; Concentration process all requires to control temperature not higher than 75 ℃, and vacuum tightness is not less than 0.085MPa.
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| CN105802855A (en) * | 2016-03-15 | 2016-07-27 | 江苏神华药业有限公司 | Method for rapidly producing antrodia comphorata powder through deep liquid state fermentation |
| CN108103124A (en) * | 2018-03-01 | 2018-06-01 | 江南大学 | A kind of liquid state fermentation of Antrodia camphorata exocellular polysaccharide and purification process |
| CN114276973A (en) * | 2021-12-03 | 2022-04-05 | 扬州大学 | Method for promoting antrodia camphorata submerged fermentation spore production and sugar production by adding antrodia camphorata extract |
| CN114276973B (en) * | 2021-12-03 | 2024-03-26 | 扬州大学 | Method for promoting deep fermentation of Antrodia camphorata to produce spores and sugar by adding Antrodia camphorata extract |
| CN114686542A (en) * | 2021-12-24 | 2022-07-01 | 上海理工大学 | Low-molecular-weight antrodia camphorata exopolysaccharide and preparation and application thereof |
| CN114686542B (en) * | 2021-12-24 | 2024-04-26 | 上海理工大学 | Low molecular weight antrodia camphorate extracellular polysaccharide and preparation and application thereof |
| CN114574420A (en) * | 2022-03-31 | 2022-06-03 | 扬州大学 | Method for promoting asexual spore production of antrodia camphorata by submerged fermentation, culture medium and application thereof |
| CN114574420B (en) * | 2022-03-31 | 2023-11-21 | 扬州大学 | Method for promoting asexual spore production of Antrodia camphorata through submerged fermentation and culture medium and application thereof |
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