CN104087631B - Method for producing Antrodia camphorata extracellular polysaccharides by deep liquid fermentation - Google Patents
Method for producing Antrodia camphorata extracellular polysaccharides by deep liquid fermentation Download PDFInfo
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Abstract
The invention discloses a method for producing Antrodia camphorata extracellular polysaccharides by deep liquid fermentation, particularly an industrial-scale production method of Antrodia camphorata extracellular polysaccharides. The method comprises the following steps: inoculating activated Antrodia camphorata strain into a seed tank to perform amplification culture, inoculating the liquid seed into a purpose-made polysaccharide-producing liquid culture medium to perform fermentation, separating the fermentation liquid, and extracting the extracellular polysaccharides. In order to provide the yield of the extracellular polysaccharides, the method optimizes the formula of the fermentation culture medium, and adopts the compound formula against the carbon source part, thereby ensuring the biomass of the earlier-stage thallus and also ensuring the yield of the later-stage metabolites. The method can be used for preparing high-quality polysaccharides with different contents, and the polysaccharide content can be 20-50%.
Description
Technical field
The invention belongs to microbial technology field, and in particular to a kind of deep layer liquid fermentation produces the side of Antrodia camphorata extracellular polysaccharide
Method.
Background technology
Antrodia camphorata also known as Camphor tree life Antrodia, Antrodia camphorata, Antrodia Camphorata, red Antrodia camphorata, Camphor tree mushroom, Camphor tree wild rice, wild rice in Camphor tree cave, red Camphor tree is subordinate to
Eumycota, Basidiomycotina, Hymenomyceteses, Polyporaceae, the perennial Mushrooms of antrodia karst are that the peculiar one kind in Taiwan is rare, not yet
The famous and precious pharmaceutical fungi and edible fungi of exploitation.Only it is grown in distinctive cinnamomum kanehirai trunk between 450～2000 meters of Taiwan mountain area height above sea level rotten
Heartwood inwall, or withered lodging Cinnamomum kanahirai hay timber darkness humidity surface, find be difficult really.Antrodia camphorata is distinctive as Taiwan
Strain, cinnamomum kanehirai is its unique host in natural environment, and general funguses can not grow thereon.Because Antrodia camphorata can cause Cinnamomum kanahirai hay
Tree central cavity is rotten, adds people to cinnamomum kanehirai excessive and random lumbering, causes cinnamomum kanehirai quantity quite rare, has been listed in one-level at present
Child care seeds.In the last hundred years, cinnamomum kanehirai is rare to see, and the cinnamomum kanehirai that can grow Antrodia Camphorata is then less, and its result causes open country
Raw Antrodia camphorata resource critical shortage.Therefore Antrodia camphorata is very precious, and in Hong Kong, Macao and Taiwan " Ganoderma " is referred to as, and Taiwan is among the people, and Antrodia camphorata is called
" ruby in forest ".
In recent years, research learns Antrodia camphorata energy effectively treatment hepatopathy and other side such as：Antitumor, antiviral, antiallergic, drop
Blood fat, hypoglycemic disease.Contain various physiologically active ingredients, such as polysaccharide, triterpenoid compound, sudismase in Antrodia camphorata
(SOD), adenosine, protein (contain immune protein), multivitamin, trace element (calcium, phosphorus), nucleic acid, agglutinin, aminoacid,
Cholesterol, lignin, blood pressure stabilization material etc..Research finds that Antrodia camphorata polysaccharide is the main active substances in Antrodia camphorata, and it is in Antrodia camphorata
Content in fermented supernatant fluid is up to 3%, with immune physiologically active, prevention and the control physiologically active such as tumor, mainly containing β-
D-glucan (callose)) its effect is through exciting macrophage, T lymphocytes, bone-marrow-derived lymphocyte and natural killer
Cell etc. reaches antineoplastic effect strengthening immunologic function.In addition Antrodia camphorata polysaccharide also have heart tonifying, blood pressure lowering, blood sugar lowering,
The effects such as antithrombotic.
The domestic and international research to Antrodia camphorata at present is not also very thorough, and the main culture method taken is solid fermentation method.Due to
This method fermentation period is long, high labor intensive, and floor space is wide, is subject to seasonal restrictions, and easily pollutes, and should not carry out pure-blood ferment
The shortcomings of, cause Antrodia camphorata fermentation-scale to expand always.For this purpose, carrying out natural Antrodia camphorata deep liquid with biological technique method
Fermentation culture can shorten incubation time, obtain a large amount of and pure mycelium and polysaccharide, and low cost is capable of achieving fermentation industry
Serialization, automatization, lift productivity effect, become the approach of very worth exploitation.Also there is grinding for a small amount of universities and colleges both at home and abroad
Submerged fermentation of the person of studying carefully to Antrodia camphorata carried out research, but often its main emphasis is how to obtain higher Biomass, only
The research of the functional component polysaccharide of small part researcher concern Antrodia camphorata, but the shake flask test of lab scale is often all limited only to, belong to
Phase of basic research, and its Biomass and the extracellular sugared yield of activated product, than relatively low, production cost is prohibitively expensive, and its technique is needed
Further lifted, the production Antrodia camphorata polysaccharide to reach scale and industrialization also needs to carry out substantial amounts of research and experiment work
Make.
The content of the invention
The invention aims to solve the technical bottleneck of industrial-scale production Antrodia camphorata polysaccharide, given birth to using liquid fermentation
Produce that Antrodia camphorata polysaccharide overcomes solid fermentation cycle length, investment is big, be subject to seasonal restrictions, be difficult to the drawbacks such as industrialization, using this invention
Can continuously, automatization, the Antrodia camphorata polysaccharide for producing high-quality of industrialization.
The purpose of the present invention is achieved through the following technical solutions：
A kind of method for preparing Antrodia camphorata fermentation liquid, the method comprises the steps：
The liquid fermentation medium that Antrodia camphorata seed culture fluid is accessed after sterilizing is carried out into deep layer liquid fermentation and obtains Antrodia camphorata
Ferment culture fluid；Described deep layer liquid fermentation inoculum concentration is 10～20% (g/100g, similarly hereinafter), and ventilating ratio is 1：0.3‐
1.0vvm, 0.02～0.05Mpa of tank pressure, cultivate 4～7 days at 22～30 DEG C；Described liquid fermentation medium is：Glucose
1-3%, starch 1-3%, peptone 0.1-0.5%, wheat bran 0.1-0.5%, potassium dihydrogen phosphate 0.1-0.5%, Magnesium sulfate heptahydrate
The Thermostable α-Amylase 0.001-0.007% (v/v) of 0.1-0.5%, defoamer 0.01-0.05%, 20000u/ml, is adjusted
PH value 4.8.Remaining " % " represents g/100ml in addition to high-temperatureα-amylase in above-mentioned culture medium composition.
Described defoamer can be the materials such as vegetable oil (Oleum Glycines, Oleum Brassicae campestriss) class, material of organic ethers.
Wherein described Antrodia camphorata seed culture fluid is preferably obtained by two grades of cultures, and concrete grammar is as follows：
1) first order seed culture：Activated shaking flask strain is accessed into the liquid seed culture medium after sterilizing, inoculum concentration is 0.1
～2%, ventilating ratio is 1：0.3-1.0vvm, 0.02～0.05Mpa of tank pressure, cultivate 6～8 days at 22～30 DEG C, obtain Antrodia camphorata
First order seed culture fluid, it is standby；
2) secondary seed culture：By step 1) obtained by first order seed culture fluid access sterilizing after liquid seeds culture
Base, inoculum concentration is 10～20%, and ventilating ratio is 1：0.3-1.0vvm, 0.02～0.05Mpa of tank pressure, at 22～30 DEG C 4 are cultivated
～7 days, obtain described Antrodia camphorata seed culture fluid.
Described liquid seed culture medium is preferably：Glucose 2-4%, peptone 0.1-0.5%, yeast powder 1-3%, phosphorus
Acid dihydride potassium 0.1-0.5%, Magnesium sulfate heptahydrate 0.1-0.5%, defoamer 0.01-0.05%, adjust pH value 4.8.First order seed
The volume of tank is 100-500L, and the volume of secondary seed tank is 1000-5000L, and loadings are 70-90%.Above-mentioned culture is basis set
" % " in represents g/100ml.
Fermentation liquid culture endpoint standard：It is muddy that fermentation liquid gives off a strong fragrance, fungus ball becomes broken, filtrate；PH value, reducing sugar drop
To minimum and slightly fluctuate, pH value 3.0-4.0, reducing sugar 1.0% (g/100g) is below；Microscopy mycelia is in small, broken bits, a large amount of spores, nothing
Living contaminantses.
A kind of method that Antrodia camphorata extracellular polysaccharide is extracted, comprises the steps：
1) separate：By the Antrodia camphorata separation of fermentative broth for obtaining according to the method described above, Antrodia camphorata filtrate is obtained；
2) concentrate：By step 1) obtained by filtrate carry out using two grade low-temps concentrate, obtain concentrate proportion be 1.25～
The concentrated pulp of 1.40 (50 DEG C of heat are surveyed).
3) precipitate with ethanol, drying：95% ethanol for adding concentrated pulp weight 3-5 times to measure, with alcohol meter mixed liquor alcoholic strength is detected
About 65-80% (v/v), stands 8-16h, and precipitate is carried out into vacuum drying, and temperature 50-75 DEG C, vacuum is not less than
0.085MPa；It is 3～8% (g/100g) to dry to dried object moisture, obtains final product extracellular sugar.
Described separation is preferably carried out using horizontal screw centrifuge, and rotating speed is controlled in 2500-3500r/min.
Step 2) secondary concentration that adopted for：One-level is concentrated using 1-3t double effect evaporators, and fermentation liquid is concentrated to into ratio
Weight is 1.15-1.20 (50 DEG C heat survey), two grades using the concentration of 1-3t haplo-effect concentrators, will proportion be 1.1-1.20 (50 DEG C of heat
Survey) concentrated solution continue to be concentrated to proportion for 1.25～1.40 (50 DEG C of heat are surveyed) concentrated pulp；Concentration process is required to control temperature
Not higher than 75 DEG C of degree, vacuum is not less than 0.085MPa.
Precipitate with ethanol is that, using 1-3t Alcohol-settling tanks, precipitate with ethanol number of times is more in the present invention, and the content of extracellular sugar is higher.Vacuum drying is adopted
Vacuum drying oven, the extracellular sugared content that the present invention is extracted is in 20-50%.
The present invention has the advantages that following protrusion：
The present invention is exactly directed to and is amplified to what the technical barrier run in industrialized production was studied by lab scale, in lab scale
Continue to optimize fermentation medium (especially carbon source) with pilot scale, lifted on the basis of Biomass and extracellular polysaccharide, solve amplification
During the difficult problem such as the Fermentation Process of Parameter control, extraction equipment type selecting, the optimization that run into, be finally successfully grafted onto 10-20t fermentations
Tank carries out industrialization production, and smoothly produce the Antrodia camphorata polysaccharide of high-quality, is truly realized continuous, automatization, industrialization
Production.
In order to improve the yield of purpose product extracellular polysaccharide, the present invention is optimized and group in the carbon source of fermentation medium
Close:Using short-acting glucose and the compound prescription of long-acting starch.Earlier fermentation is improved based on biological content with growing mycelium,
Therefore the glucose carbon source that thalline is preferentially selected, rapid propagation are with the addition of in culture medium；The fermentation later stage will improve purpose product
Content, therefore carbon source provides thalline relatively using slower starch, and add appropriate amount of starch enzyme in the medium, control thalline number
Amount gives birth to the extracellular sugar of metabolite in proper size, not pregnancy ceased.
In order to provide the yield of extracellular sugar, the formula of present invention optimization fermentation medium is employed multiple for carbon source part
Charge-coupled side, both ensure that the Biomass of early stage thalline, and the yield of later stage metabolite is ensured again.
The polysaccharide of the high-quality of different content according to the market demand and product standard, can be obtained using the present invention, polysaccharide contains
Amount can be in 20-50%.
Specific embodiment
The present invention is further illustrated below by way of specific embodiment.But the detail of embodiment is only used for explaining this
It is bright, it should not be construed as limited overall technical solution.
The Antrodia camphorata bacterial strain that following examples are used is bought in institute of microbiology of Chinese Academy of Sciences common micro-organisms center, and strain is compiled
Code CGMCC NO.0543, by taking this plant of bacterium as an example, describe the concrete scheme of the present invention in detail.But the inventive method is common to institute
Some Wu Ling ginseng bacterium, are not limited in the bacterial strain.
Embodiment 1
The method of industrialization deep layer liquid fermentation production Antrodia camphorata extracellular polysaccharide of the present invention is carried out in the steps below：
1st, the preparation of Antrodia camphorata fermentation liquid：The Antrodia camphorata shaking flask bacterial strain for having activated is inoculated in into seed culture medium by inoculum concentration 1%
(300L), wherein seed culture medium consists of glucose 3%, peptone 0.2%, yeast powder 1%, potassium dihydrogen phosphate 0.3%, seven
Water magnesium sulfate 0.15%, defoamer 0.02%, with dilute sulfuric acid pH value 4.8 is adjusted, and ventilating ratio is 1：0.3vvm, tank pressure 0.03Mpa,
26～28 DEG C of shaken cultivation obtain final product Antrodia camphorata primary seed solution in 7 days；Primary seed solution is accessed into secondary seed training by inoculum concentration 15%
Foster base (3000L), wherein secondary seed medium consist of glucose 3%, peptone 0.2%, yeast powder 1%, biphosphate
Potassium 0.3%, Magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, with dilute sulfuric acid pH value 4.8 is adjusted, and ventilating ratio is 1：0.3vvm, tank
Pressure 0.03Mpa, 26～28 DEG C of cultures obtain final product Antrodia camphorata secondary seed solution in 5 days；Secondary seed solution is accessed into fermentation by inoculum concentration 18%
Culture medium (7500L), wherein fermentation medium consist of glucose 2%, starch 2%, peptone 0.3%, wheat bran 0.3%, phosphorus
Acid dihydride potassium 0.2%, Magnesium sulfate heptahydrate 0.2%, Oleum Glycines 0.03%, Thermostable α-Amylase (the biological limited public affairs of Zaozhuang outstanding person's promise enzyme
Department, 20000u/ml) 0.002% (v/v), adjust pH value 4.8 with dilute sulfuric acid；Ventilating ratio is 1：0.5vvm, tank pressure 0.03Mpa, 26
～28 DEG C are cultivated 7 days, ferment to fermentation liquid give off a strong fragrance (milk fragrant smell), that fungus ball becomes broken, filtrate is muddy；PH value is minimized
3.2 and slightly fluctuate, reducing sugar minimizes 0.6 and slightly fluctuation；Microscopy mycelia is in small, broken bits, a large amount of spores, without living contaminantses, i.e.,
Obtain Antrodia camphorata fermentation liquid 8850Kg.
2nd, Antrodia camphorata separation of fermentative broth：Above-mentioned Antrodia camphorata fermentation liquid 8850Kg is separated by horizontal screw centrifuge, Camphor tree is obtained
The wet mycelium (water content is 75%) of sesame filtrate 8400Kg and 350Kg, wet mycelium directly cold drying can crush prepared sending out
Ferment Antrodia camphorata mycopowder.Concrete technology condition is：Rotating speed is controlled in 3000r/min, and disengaging time is in 2h.
4th, cryoconcentration：The Antrodia camphorata filtrate 8400Kg vacuum of step 2 gained is transferred to into 3t double effect evaporators, in vacuum
For 0.085MPa, thickening temperature is to be concentrated into proportion under the conditions of 65 DEG C for 1.20 (50 DEG C of heat are surveyed) concentrated solutions, then concentrated solution is true
Idle running move to 1.5t single-action ball-type concentrators, vacuum be 0.09MPa, thickening temperature be 60 DEG C under the conditions of continue to be concentrated into ratio
Weight is concentrated pulp 300kg of 1.30 (50 DEG C of heat are surveyed).
5th, precipitate with ethanol, drying：95% ethanol of weight 1000kg is added in the Alcohol-settling tank for filling 300kg concentrated pulp, wine is used
Essence meter detection mixed liquor alcoholic strength 72% (v/v), stands 12h, and bottom sediment separation is dried with vacuum drying oven, temperature
60 DEG C of degree, vacuum 0.09MPa；Drying 15h obtains extracellular sugared 75kg.Detection moisture is 5%, and polyoses content is 32%.
Embodiment 2
1st, the preparation of Antrodia camphorata fermentation liquid：The Antrodia camphorata shaking flask bacterial strain for having activated is inoculated in into seed culture by inoculum concentration 1.5%
Base (300L), wherein seed culture medium consist of glucose 3%, peptone 0.2%, yeast powder 1%, potassium dihydrogen phosphate 0.3%,
Magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, with dilute sulfuric acid pH value 4.8 is adjusted, and ventilating ratio is 1：0.3vvm, tank pressure
0.03Mpa, 26～28 DEG C of shaken cultivation obtain final product Antrodia camphorata primary seed solution in 8 days；Primary seed solution is accessed into two by inoculum concentration 15%
Level seed culture medium (3000L), wherein secondary seed medium consist of glucose 3%, peptone 0.2%, yeast powder 1%,
Potassium dihydrogen phosphate 0.3%, Magnesium sulfate heptahydrate 0.15%, defoamer 0.02%, with dilute sulfuric acid pH value 4.8 is adjusted, and ventilating ratio is 1：
0.3vvm, tank pressure 0.03Mpa, 26～28 DEG C of cultures obtain final product Antrodia camphorata secondary seed solution in 6 days；Secondary seed solution is pressed into inoculum concentration 18%
Fermentation medium (15000L) is accessed, wherein fermentation medium consists of glucose 2%, starch 2%, peptone 0.3%, wheat bran
0.3%th, potassium dihydrogen phosphate 0.2%, Magnesium sulfate heptahydrate 0.2%, Oleum Brassicae campestriss 0.03%, Thermostable α-Amylase (Zaozhuang outstanding person's promise enzyme
Biological company limited, 20000u/ml) 0.002% (v/v), adjust pH value 4.8 with dilute sulfuric acid；Ventilating ratio is 1：0.5vvm, tank pressure
0.04Mpa, 26～28 DEG C are cultivated 7 days, ferment to fermentation liquid give off a strong fragrance (have obvious milk fragrant smell), fungus ball becomes broken, filtrate
It is muddy；PH value minimizes 3.0 and slightly fluctuates, and reducing sugar minimizes 0.4 and slightly fluctuates；Microscopy mycelia is in small, broken bits, in a large number
Spore, without living contaminantses, had both obtained Antrodia camphorata fermentation liquid 17700Kg.
2nd, the separation of Antrodia camphorata fermentation liquid：Above-mentioned Antrodia camphorata fermentation liquid 17700Kg is separated by horizontal screw centrifuge, is obtained
The wet mycelium (water content is 78%) of Antrodia camphorata filtrate 16750Kg and 800Kg, wet mycelium directly cold drying can crush prepared
Fermentation Antrodia camphorata mycopowder.Concrete technology condition is：Rotating speed is controlled in 3200r/min, and disengaging time is in 4.5h.
4th, cryoconcentration：The Antrodia camphorata filtrate 16750Kg vacuum of step 2 gained is transferred to into 3t double effect evaporators, in vacuum
Spend for 0.09MPa, thickening temperature is to be concentrated into proportion under the conditions of 63 DEG C for 1.18 (50 DEG C of heat are surveyed) concentrated solutions, then concentrated solution is true
Idle running moves to 1.5t single-action ball-type concentrators, is 0.09MPa in vacuum, and thickening temperature is to be concentrated into proportion under the conditions of 65 DEG C
Concentrated pulp 550kg of 1.35 (50 DEG C of heat are surveyed).
5th, precipitate with ethanol, drying：95% ethanol of weight 2200kg is added in the Alcohol-settling tank for filling 550kg concentrated pulp, wine is used
Essence meter detection mixed liquor alcoholic strength 70% (v/v), stands 10h, and bottom sediment separation is dried with vacuum drying oven, temperature
65 DEG C of degree, vacuum -0.09MPa；Drying 18h obtains extracellular sugared 132kg.Detection moisture is 4%, and polyoses content is 35%.
Embodiment 3
It is the compound prescription of short-acting glucose and long-acting starch more preferably in the checking present invention in industrialization production Antrodia camphorata polysaccharide
Superiority, several contrast tests have been done again.Only have adjusted in test in liquid fermentation medium carbon source glucose or (and) form sediment
The consumption of powder, does not contain starch in comparative example 1, concentration of glucose is 2.0%；Glucose, starch concentration are not contained in comparative example 2
For 2.5%, glucose content is 2.5% in comparative example 3, and content of starch is 0.5%；Remaining condition with the embodiment of the present invention 1,
Result of the test is shown in Table 1：
Table 1
Claims (7)
1. a kind of method for preparing Antrodia camphorata fermentation liquid, it is characterised in that the method comprises the steps：
The liquid fermentation medium that Antrodia camphorata seed culture fluid is accessed after sterilizing is carried out into deep layer liquid fermentation and obtains Antrodia camphorata fermentation training
Nutrient solution；Described deep layer liquid fermentation inoculum concentration is 10 ~ 20 g/100g, and ventilating ratio is 1：0.3-1.0vvm, tank pressure 0.02～
0.05Mpa, cultivates 4 ~ 7 days at 22 ~ 30 DEG C；Described liquid fermentation medium is：Glucose 1-3%, starch 1-3%, albumen
Peptone 0.1-0.5%, wheat bran 0.1-0.5%, potassium dihydrogen phosphate 0.1-0.5%, Magnesium sulfate heptahydrate 0.1-0.5%, defoamer 0.01-
0.05%th, the Thermostable α-Amylase 0.001-0.007% v/v of 20000u/ml, adjust pH value 4.8.
2. the method for preparing Antrodia camphorata fermentation liquid according to claim 1, it is characterised in that described Antrodia camphorata seed culture fluid
Obtained by two grades of cultures, concrete grammar is as follows：
1）First order seed culture：Activated shaking flask strain is accessed into the liquid seed culture medium after sterilizing, inoculum concentration is 0.1 ~ 2%,
Ventilating ratio is 1：0.3-1.0vvm, 0.02～0.05Mpa of tank pressure, cultivate 6 ~ 8 days at 22 ~ 30 DEG C, obtain Antrodia camphorata first order seed
Culture fluid, it is standby；
2）Secondary seed culture：By step 1）The first order seed culture fluid of gained accesses the liquid seed culture medium after sterilizing, connects
The amount of kind is 10 ~ 20%, and ventilating ratio is 1：0.3-1.0vvm, 0.02～0.05Mpa of tank pressure, cultivate 4 ~ 7 days at 22 ~ 30 DEG C, obtain
Described Antrodia camphorata seed culture fluid.
3. the method for preparing Antrodia camphorata fermentation liquid according to claim 2, is characterized in that described liquid seed culture medium is：
Glucose 2-4%, peptone 0.1-0.5%, yeast powder 1-3%, potassium dihydrogen phosphate 0.1-0.5%, Magnesium sulfate heptahydrate 0.1-0.5%, disappear
Infusion 0.01-0.05%, adjusts pH value 4.8.
4. the method for preparing Antrodia camphorata fermentation liquid according to claim 1, is characterized in that the culture terminal of deep layer liquid fermentation：
It is muddy that fermentation liquid gives off a strong fragrance, fungus ball becomes broken, filtrate；PH value, reducing sugar minimize and slightly have fluctuation, and pH value 3.0-4.0 goes back
Below the g/100g of raw sugar 1.0；Microscopy mycelia is in small, broken bits, a large amount of spores, without living contaminantses.
5. a kind of method that Antrodia camphorata extracellular polysaccharide is extracted, it is characterised in that the method comprises the steps：
1）Separate：The Antrodia camphorata fermentation liquid of claim 1 gained is separated, Antrodia camphorata supernatant is obtained；
2）Concentration：By step 1）Gained supernatant using two grade low-temps concentrate, obtain concentration proportion be 1.25～1.40 it is dense
Contracting slurry；
3）Precipitate with ethanol, drying：95% ethanol for adding concentrated pulp weight 3-5 times to measure, is 65- with alcohol meter detection mixed liquor alcoholic strength
80%, 8-16h is stood, precipitate is carried out into vacuum drying, temperature 50-75 DEG C, vacuum is not less than 0.085MPa；Dry to dry
Dry thing moisture is 3～8 g/100g, obtains final product extracellular sugar.
6. the method that Antrodia camphorata extracellular polysaccharide according to claim 5 is extracted, it is characterised in that the step 1）Described in
Separation is carried out using horizontal screw centrifuge, and rotating speed is controlled in 2500-3500r/min.
7. the method that Antrodia camphorata extracellular polysaccharide according to claim 5 is extracted, it is characterised in that the step 2）Employed in
Secondary concentration be：One-level is concentrated using double effect evaporator, and it is 1.15-1.20 that fermentation liquid is concentrated to into proportion, and two grades using single
Effect concentrator concentration, will proportion continue to be concentrated to the concentrated pulp that proportion is 1.25～1.40 for the concentrated solution of 1.1-1.20；It is dense
Compression process is required to control temperature and is not higher than 75 DEG C, and vacuum is not less than 0.085MPa.
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| CN104278064A (en) * | 2014-10-20 | 2015-01-14 | 中山安荞生物科技有限公司 | Extracting method of antrodia polysaccharides |
| CN104909873A (en) * | 2015-05-13 | 2015-09-16 | 柳州市耕青科技有限公司 | Culture solution used for culturing Antrodia camphorate hyphae through liquid fermentation method |
| CN105802855A (en) * | 2016-03-15 | 2016-07-27 | 江苏神华药业有限公司 | Method for rapidly producing antrodia comphorata powder through deep liquid state fermentation |
| CN108103124A (en) * | 2018-03-01 | 2018-06-01 | 江南大学 | A kind of liquid state fermentation of Antrodia camphorata exocellular polysaccharide and purification process |
| CN114276973B (en) * | 2021-12-03 | 2024-03-26 | 扬州大学 | Method for promoting deep fermentation of Antrodia camphorata to produce spores and sugar by adding Antrodia camphorata extract |
| CN114686542B (en) * | 2021-12-24 | 2024-04-26 | 上海理工大学 | Low molecular weight antrodia camphorate extracellular polysaccharide and preparation and application thereof |
| CN114574420B (en) * | 2022-03-31 | 2023-11-21 | 扬州大学 | Method for promoting asexual spore production of Antrodia camphorata through submerged fermentation and culture medium and application thereof |
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