CN102021212A - Preparation method of ganoderma polysaccharide - Google Patents
Preparation method of ganoderma polysaccharide Download PDFInfo
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- CN102021212A CN102021212A CN200910176885XA CN200910176885A CN102021212A CN 102021212 A CN102021212 A CN 102021212A CN 200910176885X A CN200910176885X A CN 200910176885XA CN 200910176885 A CN200910176885 A CN 200910176885A CN 102021212 A CN102021212 A CN 102021212A
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- fermentation
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- ganoderma
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Abstract
The invention relates to the technical field of fungal fermentation. Domestic funguses and other microbes are cultured in liquid nutrient solution, so deep culture is also known as sinking culture or liquid fermentation. The fermentation production method is carried out in large-scale fermentation cylinder, and large numbers of fungi mycelium can be obtained in short time through adjusting medium composition, fermentation temperature, fermentation time and so on. Ganoderma strains in the invention are subjected to seed culture, shake culture, and fermentor culture, fermentation broth is separated by high-speed centrifugal, filtrate obtained from fermentation broth after centrifugation is filtered by filter membrane, anhydrous alcohol is added after the resulting filtrate is condensed the solution is allowed to stand overnight under 4 DEG C to precipitate, and ethanol precipitation liquid is separated by high speed centrifugation to obtain the precipitate ganoderma polysaccharide.
Description
Technical field
The present invention relates to the fungi fermentation field.Because microorganisms such as edible mushrooms grow in liquid medium, so deep layer is cultivated, and having another name called sinks cultivates or liquid fermenting.The method of cultivating the fungi entity with traditional farmer's law is different, and fermentative Production is to carry out in large fermentation tank, can obtain a large amount of radicula byssoideas at short notice by regulating substratum composition, leavening temperature and time etc.
Background technology
The submerged fermentation of edible fungus grows up on the antibiotic fermentation technical foundation.1948, the non-the first chief executive of Special Administrative Region of the Durham of the U.S. proposed earlier to cultivate mushroom mycelium with the deep layer method.Non-spy of 1948-1954 Durham and his colleagues have selected three strains and have been fit to the mushroom mycopremna that deep layer is cultivated.Nineteen fifty-three, doctor Bu Luoke of the U.S. has cultivated wild mushroom with useless mandarin orange juice deep layer.1958, picogram this first was turned out the morel mycelium pellet in fermentor tank, and had made the production test of 25000 gallons of scales.From then on the cultivation of edible mushrooms begins to have stepped into industrial field from agriculture production.China is edible or medicinal fungus since the production of wide-scale adoption at end of the sixties tank fermentation method, mainly is used on the medicine industry.Over nearly 20 years, increasingly mature along with this respect technology begins to be used for producing functional foodstuff gradually.
At present, the Ganoderma mycelium of liquid towards cultivation both at home and abroad all has bibliographical information.Tang Yajie[3] etc. the people carbon source influenced glossy ganoderma mycelium fermentation output study, they discover by batch feeding and cultivate the polysaccharide and the Ganodenic acid that can obtain high yield, their starting point concentration that studies have shown that carbon source and sugar is bigger to the influence of Ganodenic acid and polysaccharide yield, sucrose can be used as the production that carbon source is used for exocellular polysaccharide in addition, but sucrose is unfavorable to the growth of Ganoderma mycelium, lactose also can be used as carbon source and is used for the Ganoderma lucidum mycelium bulk-growth, and lactose is to not influence of the growth of Ganoderma mycelium, and the output of Ganodenic acid and intracellular polyse is also higher; When the starting point concentration of lactose surpassed 35g/L, the output of Ganodenic acid will descend, but when the remaining quantity of lactose during at 10-5g/L, carried out the output that pulse supply lactose can significantly improve Ganodenic acid; During the fermentation, dissolved oxygen is controlled between the 20-35%, can obtains the highest Ganoderma mycelium output; The amount of exo polysaccharides and Ganodenic acid is different in the Ganoderma mycelium that stir culture and shake-flask culture obtain, the born of the same parents.
Development along with ganoderma lucidum liquid submerged fermentation technology, the fermentative Production of carrying out Ganoderma mycelium with large fermentation tank and cheap substratum can reduce the production cost of glossy ganoderma greatly, cultivate multiple ganoderma health-care food of products production and the medicine that obtains with fermentation method, be expected to make glossy ganoderma to become popular daily health products.
Summary of the invention
This patent is got fermented liquid at high speed centrifugation after Ganderma lucidum strain is carried out seed culture, shake-flask culture and fermentor cultivation, and the centrifugal back of fermented liquid gained filtrate is advanced membrane filtration, after concentrating, add dehydrated alcohol, 4 ℃ of precipitations of spending the night, the high speed centrifugation precipitation solution, collecting precipitation is ganoderan.
Embodiment
1, seed culture:
The liquid seeds cultural method: the fritter that slant strains is divided into broad bean grain size is inoculated the 2-3 piece in liquid seed culture medium, and 250mL triangular flask liquid amount 50mL places on the rotary shaking table, and 160r/min, 30 ℃ of cultivation 3-4d are standby.
2, shake-flask culture:
Cultured seed liquid is inoculated in 500mL with inoculum size 10%-15% (v/v) and shakes bottle, liquid amount 150mL, 160r/min, fermentation time 5d, 30 ℃ of temperature and initial pH6.0.Optimize by experiment, maximum polysaccharide content substratum consists of: glucose 47.4g/L, KH
2PO
43.76g/L, peptone 4.39g/L.
3,5L fermentor cultivation:
Coefficient is 0.7, and inoculum size is 10%, 30 ℃ of fermentor cultivation temperature and initial pH6.0, and air flow and mixing speed are determined as required.
4, obtain ganoderan:
Get fermented liquid 4 ℃ with 10000r/min high speed centrifugation 10min, the centrifugal back of fermented liquid gained filtrate is advanced 0.45 μ m membrane filtration, after concentrating, add 3 times of volume dehydrated alcohols, vigorous stirring, 4 ℃ of precipitations of spending the night, the high speed centrifugation precipitation solution, collecting precipitation is ganoderan.
Claims (3)
1. liquid seeds cultural method: the fritter inoculation 2-3 piece that slant strains is divided into broad bean grain size is in liquid seed culture medium, and 250mL triangular flask liquid amount 50mL places on the rotary shaking table, and it is standby that 160r/min, 30 ℃ cultivate 3-4d.
2. shake-flask culture method: cultured seed liquid is inoculated in 500mL with inoculum size 10%-15% (v/v) and shakes bottle, liquid amount 150mL, 160r/min.Fermentation time 5d, 30 ℃ of temperature and initial pH6.0.Optimize by experiment, maximum polysaccharide content substratum consists of: glucose 47.4g/L, KH
2PO
43.76g/L, peptone 4.39g/L.
3. obtain ganoderan: get fermented liquid 4 ℃ with 10000r/min high speed centrifugation 10min, the centrifugal back of fermented liquid gained filtrate is advanced 0.45 μ m membrane filtration, after concentrating, add 3 times of volume dehydrated alcohols, vigorous stirring, 4 ℃ of precipitations of spending the night, the high speed centrifugation precipitation solution, collecting precipitation is ganoderan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910176885XA CN102021212A (en) | 2009-09-23 | 2009-09-23 | Preparation method of ganoderma polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910176885XA CN102021212A (en) | 2009-09-23 | 2009-09-23 | Preparation method of ganoderma polysaccharide |
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| CN102021212A true CN102021212A (en) | 2011-04-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910176885XA Pending CN102021212A (en) | 2009-09-23 | 2009-09-23 | Preparation method of ganoderma polysaccharide |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102643884A (en) * | 2012-05-04 | 2012-08-22 | 苏州百趣食品有限公司 | Method for producing polysaccharide by utilizing fermentation of ganoderma |
| CN103740783A (en) * | 2013-12-06 | 2014-04-23 | 鼎正动物药业（天津）有限公司 | Ganoderma applanatum polysaccharide preparation method |
| CN104878127A (en) * | 2015-05-12 | 2015-09-02 | 苏州葛家坞生物科技有限公司 | Light-dimming liquid culturing method for improving yield of ganoderan |
| CN109251951A (en) * | 2018-12-05 | 2019-01-22 | 郑涛 | A kind of method that semicontinuous Liquid Culture efficiently produces ganoderma lucidum polysaccharide |
| CN110923150A (en) * | 2019-12-17 | 2020-03-27 | 石家庄亚特生物科技有限公司 | Method for extracting original strain from ganoderma lucidum body and producing strain by converting liquid |
-
2009
- 2009-09-23 CN CN200910176885XA patent/CN102021212A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102643884A (en) * | 2012-05-04 | 2012-08-22 | 苏州百趣食品有限公司 | Method for producing polysaccharide by utilizing fermentation of ganoderma |
| CN103740783A (en) * | 2013-12-06 | 2014-04-23 | 鼎正动物药业（天津）有限公司 | Ganoderma applanatum polysaccharide preparation method |
| CN103740783B (en) * | 2013-12-06 | 2016-08-24 | 鼎正动物药业（天津）有限公司 | A kind of preparation method of Ganoderma applanatum polysaccharide |
| CN104878127A (en) * | 2015-05-12 | 2015-09-02 | 苏州葛家坞生物科技有限公司 | Light-dimming liquid culturing method for improving yield of ganoderan |
| CN109251951A (en) * | 2018-12-05 | 2019-01-22 | 郑涛 | A kind of method that semicontinuous Liquid Culture efficiently produces ganoderma lucidum polysaccharide |
| CN109251951B (en) * | 2018-12-05 | 2021-12-03 | 黑龙江卓健生物科技有限公司 | Method for efficiently producing ganoderma lucidum polysaccharide through semi-continuous liquid culture |
| CN110923150A (en) * | 2019-12-17 | 2020-03-27 | 石家庄亚特生物科技有限公司 | Method for extracting original strain from ganoderma lucidum body and producing strain by converting liquid |
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Application publication date: 20110420 |