CN104350953B - A kind of breeding method of blood Ganoderma - Google Patents
A kind of breeding method of blood Ganoderma Download PDFInfo
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- CN104350953B CN104350953B CN201410697916.7A CN201410697916A CN104350953B CN 104350953 B CN104350953 B CN 104350953B CN 201410697916 A CN201410697916 A CN 201410697916A CN 104350953 B CN104350953 B CN 104350953B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
The invention discloses the breeding method of a kind of blood Ganoderma, it includes that four sport technique segments cultivated by slant culture, shake-flask culture and fermentor cultivation, bacterium rod, each sport technique segment the most all includes the preparation of formula, culture medium and cultural method, especially discloses bacterium rod culture medium prescription and is prepared from by Rhizobium spp.Acacia granule 10 16%, Qinggang tree and/or Betula wood particle 7 12%, Caulis Miscanthis floriduli grass meal 4 8%, Semen Maydis powder 3 5%, wheat flour 3 5%, analysis for soybean powder 1.5 2.5%, Testa Tritici 1.4 2.0%, sucrose 0.6 0.9%, Calx 0.6 0.9% and excess water；Comparing with existing method, whole breeding method is simple, be prone to scale operation, while reducing cost to greatest extent, improves blood Ganoderma output capacity about 30%.
Description
Technical field
The present invention relates to a kind of culturing method for edible mushrooms, the breeding method of a kind of blood Ganoderma.
Background technology
Blood Ganoderma, also known as red ganoderma, wrinkle lid Amauroderma macer, Amauroderma ruda (Berk) Pat, latin name Amauroderma rude.
Belonging to Eumycota, Hymenomycetes, Aphyllophorales, Amauroderma ruda (Berk) Pat belongs to.Because of juice such as blood interior when it grows, touch with hard thing and draw
After there will be strong color, just as drop of blood, thus blood Ganoderma of gaining the name.The medical value ratio of blood Ganoderma
Other lucidum variety is well a lot, and effect is very comprehensive, is the superfine product in Ganoderma, and it has strong essence diuresis, YIN nourishing
Tonifying YANG, stasis-dispelling and pain-killing, antiphlogistic antibacterial, the clean blood of removing toxic substances, sleeping help digestion, consolidate and hold up unit, opposing pathogenic factor, anti-
The magical effect such as the most anti-ageing, skin maintenance, life lengthening.
Wild blood Ganoderma is mainly distributed on the Wuzhou in Guangxi, He Prefecture, Xiangzhou, Pingnan County in China, the most especially with
Ganoderma near Dayao Mountain is most, and additionally also there is a small amount of distribution the Yunfu in Guangdong, and natural production is the dilutest
Few, excessively pluck because of long-term again, cause wild resource to be on the verge of exhaustion.
At present, blood Ganoderma artificial culture is also in the starting stage, owing to it belongs to the product that miniclimate is derived
Kind, growth conditions is the harshest, lacks the breeding method of ripe supporting high-yield and high-efficiency.
Summary of the invention
It is an object of the invention to provide a kind of low cost, yield height, the most producible blood in greenhouse
The breeding method of Ganoderma.
The technical scheme is that the breeding method of a kind of blood Ganoderma, comprise the steps:
(1) slant culture
1. slant culture based formulas: by weight percentage, by Rhizoma Solani tuber osi 12-20%, glucose 1.2-2.0%,
Agar 1.2-2.0% and surplus Rhizobium spp.Acacia infusion are prepared from；
2. prepared by slant medium: weighs the Rhizoma Solani tuber osi being cut into small pieces after cleaning peeling in proportion, adds in proportion
Enter Rhizobium spp.Acacia infusion, decocting in water, filtration, prepare potato juice；Potato juice is proportionally added into
Agar and glucose, boil molten, filtration, prepare slant culture based sols, be dispensed into by slant culture based sols
In test tube, autoclave sterilization, prepare test tube slant culture medium；
3. slant strains is cultivated: the female kind of blood Ganoderma being inoculated in test tube slant culture medium, mycelia is covered with tiltedly
Face, selects the inclined-plane mycelium of cleaning-less bacteria infection, prepares shaking flask strain；
(2) shake-flask culture
1. shake-flask culture based formulas: by weight percentage, by Semen Maydis powder 0.8-1.2%, sucrose 1.6-2.4%,
Peptone 0.16-0.24%, yeast powder 0.24-0.36%, potassium dihydrogen phosphate 0.08-0.12%, magnesium sulfate
0.05-0.07% and excess water are prepared from；
2. prepared by Shake flask medium: weighs Semen Maydis powder in proportion and adds a small amount of water and stir, decocting in water, filtration,
Prepare corn juice, in proportion by sucrose, peptone, yeast powder, potassium dihydrogen phosphate, magnesium sulfate and remaining
Water joins mixing and stirring in corn juice, prepares Shake flask medium mixed liquor；
3. shaking flask spawn culture: shaking flask strain uses two grades of cultivations, is inoculated into shaking flask strain and trains equipped with shaking flask
Support in the first order seed bottle of base mixed liquor, be placed in shaken cultivation on bottle swingging machine；Then by long in first order seed bottle
The seed liquor of full mycelia is poured in secondary seed bottle, is placed in shaken cultivation on bottle swingging machine, when mycelia covers with branch,
Seeing bud head clamp connection on mycelia wall, mycelium pellet is many, fermentation liquid delicate fragrance, and slightly thickness, microscopy without miscellaneous bacteria,
Prepare fermentation tank strain；
(3) fermentor cultivation
1. fermentor cultivation based formulas: by weight percentage, by Semen Maydis powder 0.8-1.2%, sucrose
1.6-2.4%, yeast powder 0.15-0.25%, potassium dihydrogen phosphate 0.15-0.25%, vegetable oil 0.08-0.18%
It is prepared from excess water；
2. prepared by fermentation tank culture medium: weighs Semen Maydis powder in proportion and adds a small amount of water and stir, decocting in water, filtration,
Prepare corn juice, in proportion sucrose, yeast powder, potassium dihydrogen phosphate, vegetable oil and remaining water are joined
Mixing and stirring in corn juice, prepares fermentation tank culture medium mixed liquor；
3. fermentation tank spawn culture: fermentation tank strain use two grades of cultivations, fermentation tank strain is inoculated into equipped with
Cultivate in the first order seed fermentation tank of fermentation tank culture medium mixed liquor；Then cover with in first order seed fermentation tank
The seed liquor of mycelia is cultivated, when seed liquor is by dilute retrogradation, mycelium or mycelia in pouring secondary seed fermentation tank into
Ball is many by few change, and mycelia has clamp connection, fermentation liquid to become faint yellow, and is gradually become clear by muddiness, without miscellaneous
Bacterium pollutes, and prepares bacterium rod strain；
(4) bacterium rod is cultivated
1. bacterium rod culture medium prescription: by weight percentage, by Rhizobium spp.Acacia granule 10-16%, Qing Gangshu
And/or Betula wood particle 7-12%, Caulis Miscanthis floriduli grass meal 4-8%, Semen Maydis powder 3-5%, wheat flour 3-5%, Semen Glycines
Powder 1.5-2.5%, Testa Tritici 1.4-2.0%, sucrose 0.6-0.9%, Calx 0.6-0.9% and excess water are prepared from；
2. prepared by bacterium rod culture medium: in proportion by Rhizobium spp.Acacia granule, Qinggang tree and/or Betula timber
Grain, Caulis Miscanthis floriduli grass meal, Semen Maydis powder, wheat flour, analysis for soybean powder, Testa Tritici, sucrose, Calx and water mix and blend
Uniformly, load in tubular plastic bag, high temperature sterilize, prepare solid bacterium rod culture medium；
3. bacterium ear of maize entity is cultivated: be inoculated into by bacterium rod strain in solid bacterium rod culture medium, postvaccinal bacterium rod
Putting into culturing room, be 24 DEG C-28 DEG C in temperature, humidity is cultivation under conditions of 50-85%, timely collecting,
Obtain blood Ganoderma.
Further, described slant strains is cultivated, and cultivation temperature is 26 DEG C-28 DEG C, and incubation time is 6-8
My god.
Further, described shaking flask strain uses two grades of cultivations, and first order seed bottle is 400-600 milliliter triangular flask,
Dress Shake flask medium mixed liquor 80-120 milliliter, secondary seed bottle is 4000-6000 milliliter triangular flask, and dress shakes
Bottle culture medium mixed liquor 580-850 milliliter.
Further, described shaking flask strain uses two grades of cultivations, and first order seed bottle and secondary seed bottle are all placed in 26
Shaken cultivation at a temperature of DEG C-28 DEG C, incubation time is all 4-7 days.
Further, described fermentation tank strain uses two grades of cultivations, and first order seed fermentation tank is that 30-50 rises not
Rust cylinder of steel, fills fermentation tank culture medium mixed liquor 20-30 liter, and secondary seed fermentation tank is that 400-600 rises rustless steel
Tank, fills fermentation tank culture medium mixed liquor 240-360 liter.
Further, described fermentation tank strain uses two grades of cultivations, and first order seed fermentation tank and secondary seed are sent out
The condition of culture of ferment tank also includes stirring, ventilation, Stress control, temperature and time, and mixing speed is 220
Rev/min, ventilation is 0.5-1vvm, and tank pressure is 0.04-0.05 MPa, and temperature is 28 DEG C-30 DEG C, time
Between 40-48 hour.
Advantages of the present invention is:
The breeding method of a kind of blood Ganoderma of the present invention, including slant culture, shake-flask culture, fermentor cultivation
Cultivating four sport technique segments with bacterium rod, each sport technique segment the most all includes the preparation of formula, culture medium and cultivation side
Method, versatility, formula and method are unique, have broken cost in existing Cultivating techniques and can not reduce further,
The limitation that yield is relatively low, compares with existing method, and whole breeding method is simple, be prone to scale operation,
While reducing cost to greatest extent, improve blood Ganoderma output capacity about 30%.
Detailed description of the invention
Below by embodiment, the present invention is described in further detail:
Embodiment 1: the breeding method of a kind of blood Ganoderma, comprises the steps:
(1) slant culture
1. slant culture based formulas: by weight percentage, by Rhizoma Solani tuber osi 16%, glucose 1.6%, agar
1.6% and surplus Rhizobium spp.Acacia infusion be prepared from；
2. prepared by slant medium: weighs the Rhizoma Solani tuber osi being cut into small pieces after cleaning peeling in proportion, adds in proportion
Enter Rhizobium spp.Acacia infusion, decocting in water, filtration, prepare potato juice；Potato juice is proportionally added into
Agar and glucose, boil molten, filtration, prepare slant culture based sols, be dispensed into by slant culture based sols
In test tube, autoclave sterilization, prepare test tube slant culture medium；
3. slant strains is cultivated: the female kind of blood Ganoderma is inoculated in test tube slant culture medium, is 27 in temperature
Under conditions of DEG C, cultivate 7 days, treat that mycelia covers with inclined-plane, select the inclined-plane mycelium of cleaning-less bacteria infection, system
Obtain shaking flask strain；
(2) shake-flask culture
1. shake-flask culture based formulas: by weight percentage, by Semen Maydis powder 1%, sucrose 2%, peptone 0.2%,
Yeast powder 0.3%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.06% and excess water are prepared from；
2. prepared by Shake flask medium: weighs Semen Maydis powder in proportion and adds a small amount of water and stir, decocting in water, filtration,
Prepare corn juice, in proportion by sucrose, peptone, yeast powder, potassium dihydrogen phosphate, magnesium sulfate and remaining
Water joins mixing and stirring in corn juice, prepares Shake flask medium mixed liquor；
3. shaking flask spawn culture: shaking flask strain uses two grades of cultivations, and first order seed bottle is 500 milliliters of triangular flasks,
Dress Shake flask medium mixed liquor 100 milliliters, secondary seed bottle is 5000 milliliters of triangular flasks, fills Shake flask medium
Mixed liquor 700 milliliters；Shaking flask strain is inoculated into equipped with in the first order seed bottle of Shake flask medium mixed liquor,
First order seed bottle is placed on bottle swingging machine, shaken cultivation 6 days at a temperature of 27 DEG C；Then by first order seed bottle
The seed liquor inside covering with mycelia is poured in secondary seed bottle, and secondary seed bottle is placed on bottle swingging machine, at 27 DEG C
At a temperature of shaken cultivation 6 days；When mycelia covers with branch, mycelia wall being shown in bud head clamp connection, mycelium pellet is many,
Fermentation liquid delicate fragrance, and slightly thickness, microscopy, without miscellaneous bacteria, prepares fermentation tank strain；
(3) fermentor cultivation
1. fermentor cultivation based formulas: by weight percentage, by Semen Maydis powder 1%, sucrose 2%, yeast powder 0.2%,
Potassium dihydrogen phosphate 0.2%, Oleum Glycines 0.1% and excess water are prepared from；
2. prepared by fermentation tank culture medium: weighs Semen Maydis powder in proportion and adds a small amount of water and stir, decocting in water, filtration,
Prepare corn juice, in proportion sucrose, yeast powder, potassium dihydrogen phosphate, Oleum Glycines and water are joined in corn juice
Mixing and stirring, prepares fermentation tank culture medium mixed liquor；
3. fermentation tank spawn culture: fermentation tank strain use two grades of cultivations, first order seed fermentation tank be 40 liters not
Rust cylinder of steel, fills fermentation tank culture medium mixed liquor 24 liters, and secondary seed fermentation tank is 500 liters of stainless cylinder of steels, dress
Fermentation tank culture medium mixed liquor 300 liters；Fermentation tank strain is inoculated into equipped with fermentation tank culture medium mixed liquor
Cultivating in first order seed fermentation tank, mixing speed 220 revs/min, ventilation is 0.8vvm, and tank pressure is 0.04
MPa, temperature is 29 DEG C, incubation time 45 hours；Then the kind of mycelia will be covered with in first order seed fermentation tank
Sub-liquid is cultivated in pouring secondary seed fermentation tank into, and mixing speed 220 revs/min, ventilation is 0.8vvm, tank
Pressure is 0.05 MPa, and temperature is 29 DEG C, incubation time 45 hours；When seed liquor is by dilute retrogradation, mycelium or
Mycelium pellet is many by few change, and mycelia has clamp connection, fermentation liquid to become faint yellow, and is gradually become clear by muddiness,
Without living contaminants, prepare bacterium rod strain；
(4) bacterium rod is cultivated
1. bacterium rod culture medium prescription: by weight percentage, by Rhizobium spp.Acacia granule 13%, Qinggang trees
Material granule 9%, Caulis Miscanthis floriduli grass meal 6%, Semen Maydis powder 4%, wheat flour 4%, analysis for soybean powder 2%, Testa Tritici 1.7%, sucrose
0.8%, Calx 0.8% and excess water are prepared from；
2. prepared by bacterium rod culture medium: in proportion by Rhizobium spp.Acacia granule, Qinggang tree wood particle, Caulis Miscanthis floriduli
Grass meal, Semen Maydis powder, wheat flour, analysis for soybean powder, Testa Tritici, sucrose, Calx and water mixing and stirring, load
In tubular plastic bag, high temperature sterilize, prepare solid bacterium rod culture medium；
3. bacterium ear of maize entity is cultivated: be inoculated into by bacterium rod strain in solid bacterium rod culture medium, postvaccinal bacterium rod
Putting into culturing room, be 26 DEG C in temperature, humidity is cultivation under conditions of 65%, timely collecting, it is thus achieved that blood
Ganoderma.
Embodiment 2: the breeding method of a kind of blood Ganoderma, comprises the steps:
(1) slant culture
1. slant culture based formulas: by weight percentage, by Rhizoma Solani tuber osi 13%, glucose 1.8%, agar
1.5% and surplus Rhizobium spp.Acacia infusion be prepared from；
2. prepared by slant medium: weighs the Rhizoma Solani tuber osi being cut into small pieces after cleaning peeling in proportion, adds in proportion
Enter Rhizobium spp.Acacia infusion, decocting in water, filtration, prepare potato juice；Potato juice is proportionally added into
Agar and glucose, boil molten, filtration, prepare slant culture based sols, be dispensed into by slant culture based sols
In test tube, autoclave sterilization, prepare test tube slant culture medium；
3. slant strains is cultivated: the female kind of blood Ganoderma is inoculated in test tube slant culture medium, is 26 in temperature
Under conditions of DEG C, cultivate 8 days, treat that mycelia covers with inclined-plane, select the inclined-plane mycelium of cleaning-less bacteria infection, system
Obtain shaking flask strain；
(2) shake-flask culture
1. shake-flask culture based formulas: by weight percentage, by Semen Maydis powder 0.8%, sucrose 2.2%, peptone
0.18%, yeast powder 0.35%, potassium dihydrogen phosphate 0.08%, magnesium sulfate 0.07% and excess water are prepared from；
2. prepared by Shake flask medium: weighs Semen Maydis powder in proportion and adds a small amount of water and stir, decocting in water, filtration,
Prepare corn juice, in proportion by sucrose, peptone, yeast powder, potassium dihydrogen phosphate, magnesium sulfate and remaining
Water joins mixing and stirring in corn juice, prepares Shake flask medium mixed liquor；
3. shaking flask spawn culture: shaking flask strain uses two grades of cultivations, and first order seed bottle is 400 milliliters of triangular flasks,
Dress Shake flask medium mixed liquor 80 milliliters, secondary seed bottle is 4000 milliliters of triangular flasks, and dress Shake flask medium mixes
Close liquid 580 milliliters；Shaking flask strain is inoculated into equipped with in the first order seed bottle of Shake flask medium mixed liquor, one
Level seed bottle is placed on bottle swingging machine, shaken cultivation 7 days at a temperature of 26 DEG C；Then by first order seed bottle
The seed liquor covering with mycelia is poured in secondary seed bottle, and secondary seed bottle is placed on bottle swingging machine, the temperature of 26 DEG C
The lower shaken cultivation of degree 7 days；When mycelia covers with branch, mycelia wall being shown in bud head clamp connection, mycelium pellet is many,
Fermentation liquid delicate fragrance, and slightly thickness, microscopy, without miscellaneous bacteria, prepares fermentation tank strain；
(3) fermentor cultivation
1. fermentor cultivation based formulas: by weight percentage, by Semen Maydis powder 1.2%, sucrose 2.2%, yeast
Powder 0.18%, potassium dihydrogen phosphate 0.22%, Semen Maydis oil 0.12% and excess water are prepared from；
2. prepared by fermentation tank culture medium: weighs Semen Maydis powder in proportion and adds a small amount of water and stir, decocting in water, filtration,
Prepare corn juice, in proportion sucrose, yeast powder, potassium dihydrogen phosphate, Semen Maydis oil and water are joined corn juice
Middle mixing and stirring, prepares fermentation tank culture medium mixed liquor；
3. fermentation tank spawn culture: fermentation tank strain use two grades of cultivations, first order seed fermentation tank be 30 liters not
Rust cylinder of steel, fills fermentation tank culture medium mixed liquor 20 liters, and secondary seed fermentation tank is 400 liters of stainless cylinder of steels, dress
Fermentation tank culture medium mixed liquor 240 liters；Fermentation tank strain is inoculated into equipped with fermentation tank culture medium mixed liquor
Cultivating in first order seed fermentation tank, mixing speed 240 revs/min, ventilation is 0.5vvm, and tank pressure is 0.04
MPa, temperature is 28 DEG C, incubation time 40 hours；Then the kind of mycelia will be covered with in first order seed fermentation tank
Sub-liquid is cultivated in pouring secondary seed fermentation tank into, and mixing speed 200 revs/min, ventilation is 0.5vvm, tank
Pressure is 0.05 MPa, and temperature is 30 DEG C, incubation time 40 hours；When seed liquor is by dilute retrogradation, mycelium or
Mycelium pellet is many by few change, and mycelia has clamp connection, fermentation liquid to become faint yellow, and is gradually become clear by muddiness,
Without living contaminants, prepare bacterium rod strain；
(4) bacterium rod is cultivated
1. bacterium rod culture medium prescription: by weight percentage, by Rhizobium spp.Acacia granule 12%, Betula timber
Granule 11%, Caulis Miscanthis floriduli grass meal 8%, Semen Maydis powder 5%, wheat flour 3%, analysis for soybean powder 1.8%, Testa Tritici 1.6%, sugarcane
Sugar 0.9%, Calx 0.6% and excess water are prepared from；
2. prepared by bacterium rod culture medium: in proportion by Rhizobium spp.Acacia granule, Betula wood particle, Caulis Miscanthis floriduli grass
Powder, Semen Maydis powder, wheat flour, analysis for soybean powder, Testa Tritici, sucrose, Calx and water mixing and stirring, load cylinder
In shape plastic bag, high temperature sterilize, prepare solid bacterium rod culture medium；
3. bacterium ear of maize entity is cultivated: be inoculated into by bacterium rod strain in solid bacterium rod culture medium, postvaccinal bacterium rod
Putting into culturing room, be 25 DEG C in temperature, humidity is cultivation under conditions of 60%, timely collecting, it is thus achieved that blood
Ganoderma.
Embodiment 3: the breeding method of a kind of blood Ganoderma, comprises the steps:
(1) slant culture
1. slant culture based formulas: by weight percentage, by Rhizoma Solani tuber osi 18%, glucose 1.3%, agar
1.7% and surplus Rhizobium spp.Acacia infusion be prepared from；
2. prepared by slant medium: weighs the Rhizoma Solani tuber osi being cut into small pieces after cleaning peeling in proportion, adds in proportion
Enter Rhizobium spp.Acacia infusion, decocting in water, filtration, prepare potato juice；Potato juice is proportionally added into
Agar and glucose, boil molten, filtration, prepare slant culture based sols, be dispensed into by slant culture based sols
In test tube, autoclave sterilization, prepare test tube slant culture medium；
3. slant strains is cultivated: the female kind of blood Ganoderma is inoculated in test tube slant culture medium, is 28 in temperature
Under conditions of DEG C, cultivate 6 days, treat that mycelia covers with inclined-plane, select the inclined-plane mycelium of cleaning-less bacteria infection, system
Obtain shaking flask strain；
(2) shake-flask culture
1. shake-flask culture based formulas: by weight percentage, by Semen Maydis powder 1.2%, sucrose 1.8%, peptone
0.22%, yeast powder 0.26%, potassium dihydrogen phosphate 0.12%, magnesium sulfate 0.05% and excess water are prepared from；
2. prepared by Shake flask medium: weighs Semen Maydis powder in proportion and adds a small amount of water and stir, decocting in water, filtration,
Prepare corn juice, in proportion by sucrose, peptone, yeast powder, potassium dihydrogen phosphate, magnesium sulfate and remaining
Water joins mixing and stirring in corn juice, prepares Shake flask medium mixed liquor；
3. shaking flask spawn culture: shaking flask strain uses two grades of cultivations, and first order seed bottle is 600 milliliters of triangular flasks,
Dress Shake flask medium mixed liquor 120 milliliters, secondary seed bottle is 6000 milliliters of triangular flasks, fills Shake flask medium
Mixed liquor 850 milliliters；Shaking flask strain is inoculated into equipped with in the first order seed bottle of Shake flask medium mixed liquor,
First order seed bottle is placed on bottle swingging machine, shaken cultivation 5 days at a temperature of 28 DEG C；Then by first order seed bottle
The seed liquor inside covering with mycelia is poured in secondary seed bottle, and secondary seed bottle is placed on bottle swingging machine, at 28 DEG C
At a temperature of shaken cultivation 5 days；When mycelia covers with branch, mycelia wall being shown in bud head clamp connection, mycelium pellet is many,
Fermentation liquid delicate fragrance, and slightly thickness, microscopy, without miscellaneous bacteria, prepares fermentation tank strain；
(3) fermentor cultivation
1. fermentor cultivation based formulas: by weight percentage, by Semen Maydis powder 0.8%, sucrose 1.8%, yeast
Powder 0.22%, potassium dihydrogen phosphate 0.18%, sunflower oil 0.16% and excess water are prepared from；
2. prepared by fermentation tank culture medium: weighs Semen Maydis powder in proportion and adds a small amount of water and stir, decocting in water, filtration,
Prepare corn juice, in proportion sucrose, yeast powder, potassium dihydrogen phosphate, sunflower oil and water are joined corn juice
Middle mixing and stirring, prepares fermentation tank culture medium mixed liquor；
3. fermentation tank spawn culture: fermentation tank strain use two grades of cultivations, first order seed fermentation tank be 50 liters not
Rust cylinder of steel, fills fermentation tank culture medium mixed liquor 30 liters, and secondary seed fermentation tank is 600 liters of stainless cylinder of steels, dress
Fermentation tank culture medium mixed liquor 360 liters；Fermentation tank strain is inoculated into equipped with fermentation tank culture medium mixed liquor
Cultivating in first order seed fermentation tank, mixing speed 200 revs/min, ventilation is 1vvm, and tank pressure is 0.05
MPa, temperature is 30 DEG C, incubation time 48 hours；Then the kind of mycelia will be covered with in first order seed fermentation tank
Sub-liquid is cultivated in pouring secondary seed fermentation tank into, and mixing speed 240 revs/min, ventilation is 1vvm, tank pressure
Being 0.04 MPa, temperature is 28 DEG C, incubation time 48 hours；When seed liquor is by dilute retrogradation, mycelium or bacterium
Pompon is many by few change, and mycelia has clamp connection, fermentation liquid to become faint yellow, and is gradually become clear by muddiness, nothing
Living contaminants, prepares bacterium rod strain；
(4) bacterium rod is cultivated
1. bacterium rod culture medium prescription: by weight percentage, by Rhizobium spp.Acacia granule 14%, Qinggang trees
Material granule 4%, Betula wood particle 4%, Caulis Miscanthis floriduli grass meal 4%, Semen Maydis powder 3%, wheat flour 5%, analysis for soybean powder 2.2%,
Testa Tritici 1.8%, sucrose 0.6%, Calx 0.9% and excess water are prepared from；
2. prepared by bacterium rod culture medium: in proportion by Rhizobium spp.Acacia granule, Qinggang tree wood particle, birchwood
Material granule, Caulis Miscanthis floriduli grass meal, Semen Maydis powder, wheat flour, analysis for soybean powder, Testa Tritici, sucrose, Calx and water mixing
Stir, load in tubular plastic bag, high temperature sterilize, prepare solid bacterium rod culture medium；
3. bacterium ear of maize entity is cultivated: be inoculated into by bacterium rod strain in solid bacterium rod culture medium, postvaccinal bacterium rod
Putting into culturing room, be 27 DEG C in temperature, humidity is cultivation under conditions of 70%, timely collecting, it is thus achieved that blood
Ganoderma.
Claims (6)
1. a breeding method for blood Ganoderma, comprises the steps:
(1) slant culture
1. slant culture based formulas: by weight percentage, by Rhizoma Solani tuber osi 12-20%, glucose 1.2-2.0%,
Agar 1.2-2.0% and surplus Rhizobium spp.Acacia infusion are prepared from；
2. prepared by slant medium: weighs the Rhizoma Solani tuber osi being cut into small pieces after cleaning peeling in proportion, adds in proportion
Enter Rhizobium spp.Acacia infusion, decocting in water, filtration, prepare potato juice；Potato juice is proportionally added into
Agar and glucose, boil molten, filtration, prepare slant culture based sols, be dispensed into by slant culture based sols
In test tube, autoclave sterilization, prepare test tube slant culture medium；
3. slant strains is cultivated: the female kind of blood Ganoderma being inoculated in test tube slant culture medium, mycelia is covered with tiltedly
Face, selects the inclined-plane mycelium of cleaning-less bacteria infection, prepares shaking flask strain；
(2) shake-flask culture
1. shake-flask culture based formulas: by weight percentage, by Semen Maydis powder 0.8-1.2%, sucrose 1.6-2.4%,
Peptone 0.16-0.24%, yeast powder 0.24-0.36%, potassium dihydrogen phosphate 0.08-0.12%, magnesium sulfate
0.05-0.07% and excess water are prepared from；
2. prepared by Shake flask medium: weighs Semen Maydis powder in proportion and adds a small amount of water and stir, decocting in water, filtration,
Prepare corn juice, in proportion by sucrose, peptone, yeast powder, potassium dihydrogen phosphate, magnesium sulfate and remaining
Water joins mixing and stirring in corn juice, prepares Shake flask medium mixed liquor；
3. shaking flask spawn culture: shaking flask strain uses two grades of cultivations, is inoculated into shaking flask strain and trains equipped with shaking flask
Support in the first order seed bottle of base mixed liquor, be placed in shaken cultivation on bottle swingging machine；Then by long in first order seed bottle
The seed liquor of full mycelia is poured in secondary seed bottle, is placed in shaken cultivation on bottle swingging machine, when mycelia covers with branch,
Seeing bud head clamp connection on mycelia wall, mycelium pellet is many, fermentation liquid delicate fragrance, and slightly thickness, microscopy without miscellaneous bacteria,
Prepare fermentation tank strain；
(3) fermentor cultivation
1. fermentor cultivation based formulas: by weight percentage, by Semen Maydis powder 0.8-1.2%, sucrose
1.6-2.4%, yeast powder 0.15-0.25%, potassium dihydrogen phosphate 0.15-0.25%, vegetable oil 0.08-0.18%
It is prepared from excess water；
2. prepared by fermentation tank culture medium: weighs Semen Maydis powder in proportion and adds a small amount of water and stir, decocting in water, filtration,
Prepare corn juice, in proportion sucrose, yeast powder, potassium dihydrogen phosphate, vegetable oil and remaining water are joined
Mixing and stirring in corn juice, prepares fermentation tank culture medium mixed liquor；
3. fermentation tank spawn culture: fermentation tank strain use two grades of cultivations, fermentation tank strain is inoculated into equipped with
Cultivate in the first order seed fermentation tank of fermentation tank culture medium mixed liquor；Then cover with in first order seed fermentation tank
The seed liquor of mycelia is cultivated, when seed liquor is by dilute retrogradation, mycelium or mycelia in pouring secondary seed fermentation tank into
Ball is many by few change, and mycelia has clamp connection, fermentation liquid to become faint yellow, and is gradually become clear by muddiness, without miscellaneous
Bacterium pollutes, and prepares bacterium rod strain；
(4) bacterium rod is cultivated
1. bacterium rod culture medium prescription: by weight percentage, by Rhizobium spp.Acacia granule 10-16%, Qing Gangshu
And/or Betula wood particle 7-12%, Caulis Miscanthis floriduli grass meal 4-8%, Semen Maydis powder 3-5%, wheat flour 3-5%, Semen Glycines
Powder 1.5-2.5%, Testa Tritici 1.4-2.0%, sucrose 0.6-0.9%, Calx 0.6-0.9% and excess water are prepared from；
2. prepared by bacterium rod culture medium: in proportion by Rhizobium spp.Acacia granule, Qinggang tree and/or Betula timber
Grain, Caulis Miscanthis floriduli grass meal, Semen Maydis powder, wheat flour, analysis for soybean powder, Testa Tritici, sucrose, Calx and water mix and blend
Uniformly, load in tubular plastic bag, high temperature sterilize, prepare solid bacterium rod culture medium；
3. bacterium ear of maize entity is cultivated: be inoculated into by bacterium rod strain in solid bacterium rod culture medium, postvaccinal bacterium rod
Putting into culturing room, be 24 DEG C-28 DEG C in temperature, humidity is cultivation under conditions of 50-85%, timely collecting,
Obtain blood Ganoderma.
The breeding method of blood Ganoderma the most according to claim 1, it is characterised in that: described inclined-plane bacterium
Planting and cultivate, cultivation temperature is 26 DEG C-28 DEG C, and incubation time is 6-8 days.
The breeding method of blood Ganoderma the most according to claim 1 and 2, it is characterised in that: described shaking flask
Strain uses two grades of cultivations, and first order seed bottle is 400-600 milliliter triangular flask, fills Shake flask medium mixed liquor
80-120 milliliter, secondary seed bottle is 4000-6000 milliliter triangular flask, fills Shake flask medium mixed liquor
580-850 milliliter.
The breeding method of blood Ganoderma the most according to claim 3, it is characterised in that: described shaking flask strain
Using two grades of cultivations, first order seed bottle and secondary seed bottle are all placed in shaken cultivation at a temperature of 26 DEG C-28 DEG C,
Incubation time is all 4-7 days.
The breeding method of blood Ganoderma the most according to claim 4, it is characterised in that: described fermentation tank
Strain uses two grades of cultivations, and first order seed fermentation tank is that 30-50 rises stainless cylinder of steel, and dress fermentation tank culture medium is mixed
Closing liquid 20-30 liter, secondary seed fermentation tank is that 400-600 rises stainless cylinder of steel, fills fermentation tank culture medium mixed liquor
240-360 liter.
The breeding method of blood Ganoderma the most according to claim 5, it is characterised in that: described fermentation tank
Strain uses two grades of cultivations, and the condition of culture of first order seed fermentation tank and secondary seed fermentation tank also includes stirring
Mix, ventilate, Stress control, temperature and time, mixing speed is 220 revs/min, and ventilation is 0.5-1vvm,
Tank pressure is 0.04-0.05 MPa, and temperature is 28 DEG C-30 DEG C, and the time is 40-48 hour.
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| CN108901608A (en) * | 2017-03-21 | 2018-11-30 | 湖北悟芝堂生物科技有限公司 | The implantation methods of ganoderma lucidum |
| CN107047061B (en) * | 2017-04-21 | 2020-10-27 | 广西壮族自治区农业科学院微生物研究所 | Efficient method for cultivating lucid ganoderma and culture medium thereof |
| CN108934769B (en) * | 2018-06-26 | 2021-01-29 | 广东省微生物研究所（广东省微生物分析检测中心） | Submerged fermentation medium for fomes ruditapes, and preparation and application methods thereof |
| CN109429893A (en) * | 2018-10-18 | 2019-03-08 | 瀹垮饯 | A kind of breeding method of blood ganoderma lucidum |
| CN113767815A (en) * | 2021-09-15 | 2021-12-10 | 福州美利莎生物科技有限公司 | Ganoderma cockle seed ball, preparation method and cultivation method of ganoderma cockle |
| CN115152529A (en) * | 2022-08-18 | 2022-10-11 | 夏兴林 | Planting and cultivating method of ganoderma lucidum |
| CN115530013A (en) * | 2022-10-18 | 2022-12-30 | 广东粤微食用菌技术有限公司 | Production method of ganoderma leucocontextum liquid strain |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101692772A (en) * | 2009-10-22 | 2010-04-14 | 上海交通大学 | Glossy ganoderma cell culturing method for improving ganoderic acid content in cells |
| CN102067788A (en) * | 2010-10-25 | 2011-05-25 | 江苏安惠生物科技有限公司 | Method for breeding ganoderma lucidum strain by low-energy N<+> implantation induced mutation and bred strain thereof |
| CN103393094A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Preparation method of ganoderma lucidum mycelium slices |
| CN103650920A (en) * | 2013-12-20 | 2014-03-26 | 凌中鑫 | Method for fermenting ganoderma lucidum mycelia liquid rich in microelements |
| CN103694016A (en) * | 2013-12-05 | 2014-04-02 | 何庚挺 | Ganoderma lucidum culture medium and culture method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0947148A (en) * | 1995-08-08 | 1997-02-18 | Toshifumi Izawa | Artificial cultivation of cortinellus shiitake |
| KR101199763B1 (en) * | 2012-02-27 | 2012-11-08 | 전라남도 | A Method of Cultivation OF Sparassis crispa wiht high ßglucan |
| JP6265604B2 (en) * | 2013-02-20 | 2018-01-24 | 株式会社日健総本社 | Cultivation method of Mannentake with new physiological activity |
-
2014
- 2014-11-19 CN CN201410697916.7A patent/CN104350953B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101692772A (en) * | 2009-10-22 | 2010-04-14 | 上海交通大学 | Glossy ganoderma cell culturing method for improving ganoderic acid content in cells |
| CN102067788A (en) * | 2010-10-25 | 2011-05-25 | 江苏安惠生物科技有限公司 | Method for breeding ganoderma lucidum strain by low-energy N<+> implantation induced mutation and bred strain thereof |
| CN103393094A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Preparation method of ganoderma lucidum mycelium slices |
| CN103694016A (en) * | 2013-12-05 | 2014-04-02 | 何庚挺 | Ganoderma lucidum culture medium and culture method thereof |
| CN103650920A (en) * | 2013-12-20 | 2014-03-26 | 凌中鑫 | Method for fermenting ganoderma lucidum mycelia liquid rich in microelements |
Non-Patent Citations (2)
| Title |
|---|
| 深层发酵生产灵芝菌丝体多糖研究;魏赛金等;《江西科学》;20070615;第25卷(第3期);第292～294、301页 * |
| 灵芝多糖液体发酵条件优化;王慧杰等;《食用菌学报》;20070215;第14卷(第1期);第37～42页 * |
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