CN106929463A - A kind of high temperature resistant wild-type champignon strain artificial acclimation method - Google Patents
A kind of high temperature resistant wild-type champignon strain artificial acclimation method Download PDFInfo
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Abstract
The invention discloses a kind of high temperature resistant wild-type champignon strain artificial acclimation method, belong to edible mushroom domestication's technical field.In order to solve non-refractory during wild-type champignon artificial domesticating cultivation, the not enough problem of summer supply, the invention provides a kind of high temperature resistant wild-type champignon strain artificial acclimation method, the method is utilized and cultivates mushroom parent species, the various physiological properties of mushroom prepare different culture mediums when original seed and cultigen, and make full use of ultraviolet light and combine the care treatment that relents, so that the wild-type champignon bacterial strain for finally giving is heat-resist, the speed of growth is accelerated in hot environment, hair bacterium is good, improve the yield of wild-type champignon, the obstacle that mushroom lists particular season is broken, so that summer supply is increased substantially.
Description
Technical field
The invention belongs to edible mushroom domestication's technical field, specifically a kind of high temperature resistant wild-type champignon strain is artificial
Acclimation method.
Background technology
Mushroom (Lentinula edodes) yield occupies second in world artificial cultivation edible mushroom.With quality of the life
Raising, people start gradually to recognize the nutritive value of mushroom, due to its unique local flavor and enhancing immune function of human body
Effect, as one of favorite healthy food of people.
In recent years, because the supply in wild-type champignon market is not asked, therefore it is people that mushroom is grown into progressively change naturally from field
Work domesticating and cultivating, therefore the yield of mushroom is greatly improved, in order to more meet the growing market demand, then increasingly
Many Planting households are in order to carry the various chemical fertilizer of throughput applications and agricultural chemicals so that although mushroom production increase mushroom flavor and
Wild-type champignon farther out, healthy living theory is constantly pursued with people compared to difference, and vast consumer is more favored in wild
Mushroom, therefore wild-type champignon resource is seriously damaged on a large scale.
It is edible safer without any chemical contamination due to wild-type champignon pure natural growth under field conditions (factors), separately
On the trunk under natural environment, the amino acid that it contains more enriches one side wild-type champignon, and the distinctive local flavor of mushroom is also more
Enriching is strongly fragrant.Number of patent application discloses a kind of " mating system of the wild-type champignon " invention imitative wild for 201610715136X
Bred under environment, without any chemical substance, the wild-type champignon nutritive value bred out is higher.But it is wild under natural conditions
Mushroom fruiting body is main to be occurred to spring in autumn, belongs to middle low temperature type edible fungus, its fructification occurrence temperature under natural conditions
It it is 5-24 DEG C, for 8-20 DEG C, most mushroom main producing region summer temp is significantly larger than 25 DEG C to optimum temperature, causes summer fresh
Mushroom is insufficient, and this high temperature can cause serious injury to fragrant Xue, to all restricted effect of mycelia and sporophore growth.
Mainly it is presented with：Bacterium phenomenon is burnt, mycelia jaundice even stops growing under high temperature；Bacterium rod pollution rate rotten rod high, has a strong impact on mushroom
Yield；The economical character of mushroom fruiting body is deteriorated, and mushroom is small, cap is thin, be difficult Jian umbrellas, and although the invention solves wild-type champignon people
The problem of work domestication, but for non-refractory, the not enough problem of summer supply is not yet resolved.
The content of the invention
It is an object of the invention in view of the shortcomings of the prior art or defect, there is provided a kind of high temperature resistant wild Lentinus Edodes
Plant artificial acclimation method.
Realize that the purpose of the present invention is achieved through the following technical solutions：
A kind of high temperature resistant wild-type champignon strain artificial acclimation method, including step in detail below：
1. parent species are cultivated：Collection nature field grown 2500-2800 meter Loess Plateau of Gansu Province's height above sea level piece greatly
Then the wild-type champignon of lid thickness, short handle, 80to 90mature and non-parachute-opening makes parent species as kind of a mushroom using tissue isolation, by weight
Amount number chooses raw material：Eucalyptus consider to be worth doing 15-30 parts, Chinese chestnut bud husk 10-20 parts, glucose 5-10 parts, vitamin B2 1.5-4.5 parts,
Vitamin B6 2.5-4.5 parts, -4 parts of vitamin E2, vitamin C 1-3 parts, urea 3-6 parts, corn bran 6-11 parts, bone meal 7-12
Part, waste potato dregs 5-7 part, bean dregs 12-15 part, jasmonic 0.5-1.5 parts, expect water weight ratio be 1:1.2；The raw material is mixed
Conjunction is prepared into slant medium, is then aseptically inoculated with wild-type champignon parent species, first through culture 5 days under 25 DEG C of constant temperature, so
Heat shock culture 1-1.5 hours afterwards, then ultraviolet treatment 30-60min, 25 DEG C of renewal cultivations are replaced in after the completion for the treatment of, culture is extremely
Cover with inclined-plane；
2. original seed is cultivated：Raw material is chosen by weight：Maize pulp 30-50 parts, coronatine 3-5 parts, trehalose 1-2 parts,
Shitosan 1.5-2.5 parts, proline-4 .5-9.8 parts, epiphysin 2.4-5.2 parts, material water weight ratio is 1: 1.2；By the raw material
Pedigree seed culture medium is prepared by mixing into, aseptically, one-level parent species, every one-level parent species 5-7 bottles of original seed of switching, through 22 is accessed
5 days are cultivated in DEG C constant temperature dark, then Fluctuation temperature culture, until covering with bottle is two grades of kinds；
3. cultigen is cultivated：Raw material is chosen by weight：Lactobacillus plantarum 0.5-1.5 parts, calcium gluconae 2-5 parts,
Oxalic acid 1-3 parts, gypsum 3-9 parts, glycine betaine 1.5-2.5 parts, cotton seed hulls 8-10 parts, AMS 0.5-1 parts, expect that water weight ratio is
1: 1.2, the raw material is prepared by mixing into Cultivar culture medium, two grades of kinds are aseptically accessed, every two grades of parent species turn
5-7 bottles of cultigen is connect, through culture under 25-30 DEG C of steady temperature and illumination until covering with bottle as cultigen.
3. middle stationary temperature and illumination are respectively 28 DEG C and 4000lux to described step.
Dark culturing temperature is incremented to 44 DEG C by described Fluctuation temperature culture from 28 DEG C with 2 DEG C/h, and maintains 1- at 44 DEG C
1.5h, is replaced in 22 DEG C of renewal cultivations after the completion for the treatment of.
The temperature range of described heat shock culture is 47-50 DEG C.
Cultivation temperature constant range is 30-32 DEG C during described ultraviolet treatment.
The beneficial effects of the present invention are：Mushroom is various when mushroom parent species, original seed and cultigen are cultivated in present invention utilization
Physiological property prepares different culture mediums, and makes full use of ultraviolet light and combine the care treatment that relents so that finally give
Wild-type champignon bacterial strain it is heat-resist, the speed of growth is accelerated in hot environment, and hair bacterium is good, improves the product of wild-type champignon
Amount, has broken the obstacle that mushroom lists particular season so that summer supply is increased substantially.
Specific embodiment
Further describe the present invention by the following examples, it should be understood that these embodiments are only used for illustration
Purpose, is not limited to the present invention.
Embodiment 1
A kind of high temperature resistant wild-type champignon strain artificial acclimation method, including step in detail below：
1. parent species are cultivated：Piece big lid of the collection nature field grown in 2500 meters of Loess Plateaus of Gansu Province's height above sea level be thick,
Handle is short, the wild-type champignon of 80to 90mature and non-parachute-opening as kind of a mushroom, parent species are then made using tissue isolation, by weight
Choose raw material：Eucalyptus consider to be worth doing 15-30 parts, Chinese chestnut bud husk 10-20 parts, 5 parts of glucose, 1.5 parts of vitamin B2,2.5 parts of vitamin B6,
Vitamin E2 part, 1 part of vitamin C, 3 parts of urea, 6 parts of corn bran, 7 parts of bone meal, 5 parts of waste potato dregs, 12 parts of bean dregs, jasmine
0.5 part of acid, material water weight ratio are 1:1.2；The raw material is prepared by mixing into slant medium, is then aseptically inoculated with
Wild-type champignon parent species, first through culture 5 days under 25 DEG C of constant temperature, then heat shock culture 1-1.5 hours, then in temperature is 30 DEG C of environment
Ultraviolet treatment 30min, is replaced in 25 DEG C of renewal cultivations after the completion for the treatment of, culture is to covering with inclined-plane；
2. original seed is cultivated：Raw material is chosen by weight：30 parts of maize pulp, 3 parts of coronatine, 1 part of trehalose, shitosan
1.5 parts, proline-4 .5 parts, 2.4 parts of epiphysin, material water weight ratio are 1: 1.2；The raw material is prepared by mixing into Primary spawn
Base, aseptically, accesses one-level parent species, every one-level parent species 5-7 bottles of original seed of switching, through cultivating 5 in 22 DEG C of constant temperature dark
My god, then Fluctuation temperature culture, until covering with bottle is two grades of kinds；Described Fluctuation temperature culture is by dark culturing temperature from 2 DEG C of h-1Pass
Increase to 44 DEG C, and 1h is maintained at 44 DEG C, 22 DEG C of renewal cultivations are replaced in after the completion for the treatment of.
3. cultigen is cultivated：Raw material is chosen by weight：0.5 part of Lactobacillus plantarum, 2 parts of calcium gluconae, oxalic acid 1
Part, 3 parts of gypsum, 1.5 parts of glycine betaine, 8 parts of cotton seed hulls, 0.5 part of AMS, material water weight ratio is 1: 1.2, and the raw material is mixed
Conjunction is prepared into Cultivar culture medium, aseptically accesses two grades of kinds, every two grades of parent species 5-7 bottles of cultigen of switching, through 25
Culture under DEG C steady temperature and illumination until cover with bottle as cultigen, described stationary temperature and illumination be respectively 28 DEG C and
4000lux。
Embodiment 2
A kind of high temperature resistant wild-type champignon strain artificial acclimation method, including step in detail below：
1. parent species are cultivated：Piece big lid of the collection nature field grown in 2800 meters of Loess Plateaus of Gansu Province's height above sea level be thick,
Handle is short, the wild-type champignon of 80to 90mature and non-parachute-opening as kind of a mushroom, parent species are then made using tissue isolation, by weight
Choose raw material：30 parts of Eucalyptus bits, 20 parts of Chinese chestnut bud husk, 10 parts of glucose, 4.5 parts of vitamin B2,4.5 parts of vitamin B6, dimension life
Plain E4 parts, 3 parts of vitamin C, 6 parts of urea, 11 parts of corn bran, 12 parts of bone meal, 7 parts of waste potato dregs, 15 parts of bean dregs, jasmonic
1.5 parts, material water weight ratio be 1:1.2；The raw material is prepared by mixing into slant medium, is then aseptically inoculated with wild
Raw mushroom parent species, are first cultivated 5 days through under 25 DEG C of constant temperature, and then heat shock culture 1.5 hours, then ultraviolet treatment 60min, treatment is completed
After be replaced in 25 DEG C of renewal cultivations, culture is to covering with inclined-plane；The temperature of described heat shock culture is 50 DEG C；Described purple
Cultivation temperature is 32 DEG C during outer treatment.
2. original seed is cultivated：Raw material is chosen by weight：50 parts of maize pulp, 5 parts of coronatine, 2 parts of trehalose, shitosan
2.5 parts, 9.8 parts of proline, 5.2 parts of epiphysin, material water weight ratio be 1: 1.2；The raw material is prepared by mixing into Primary spawn
Base, aseptically, access one-level parent species, every one-level parent species 7 bottles of original seeds of switching, through being cultivated 5 days in 22 DEG C of constant temperature dark,
Then Fluctuation temperature culture, until covering with bottle is two grades of kinds；Described Fluctuation temperature culture is by dark culturing temperature from 2 DEG C of h-1It is incremented to
44 DEG C, and 1.5h is maintained at 44 DEG C, 22 DEG C of renewal cultivations are replaced in after the completion for the treatment of.
3. cultigen is cultivated：Raw material is chosen by weight：1.5 parts of Lactobacillus plantarum, 5 parts of calcium gluconae, oxalic acid 3
Part, 9 parts of gypsum, 2.5 parts of glycine betaine, 10 parts of cotton seed hulls, 1 part of AMS, material water weight ratio is 1: 1.2, and the raw material is mixed
Conjunction is prepared into Cultivar culture medium, aseptically accesses two grades of kinds, every two grades of parent species, 7 bottles of cultigens of switching, through 30 DEG C
Culture is until cover with bottle as cultigen under steady temperature and illumination；Described stationary temperature and illumination be respectively 28 DEG C and
4000lux。
Embodiment 3
A kind of high temperature resistant wild-type champignon strain artificial acclimation method, including step in detail below：
1. parent species are cultivated：Piece big lid of the collection nature field grown in 2650 meters of Loess Plateaus of Gansu Province's height above sea level be thick,
Handle is short, the wild-type champignon of 80to 90mature and non-parachute-opening as kind of a mushroom, parent species are then made using tissue isolation, by weight
Choose raw material：22.5 parts of Eucalyptus bits, 15 parts of Chinese chestnut bud husk, 7.5 parts of glucose, vitamin B23 parts, vitamin B63.5 parts, dimension life
Plain E3 parts, Catergen part, 4.5 parts of urea, 8.5 parts of corn bran, 9.5 parts of bone meal, 6 parts of waste potato dregs, 13.5 parts of bean dregs,
1 part of jasmonic, material water weight ratio are 1:1.2；The raw material is prepared by mixing into slant medium, is then aseptically connect
Wild-type champignon parent species are planted, is first cultivated 5 days through under 25 DEG C of constant temperature, then heat shock culture 75min, then ultraviolet treatment 45min has been processed
It is replaced in 25 DEG C of renewal cultivations after, culture is to covering with inclined-plane；The temperature of described heat shock culture is 48.5 DEG C；It is described
Ultraviolet treatment when cultivation temperature be 31 DEG C.
2. original seed is cultivated：Raw material is chosen by weight：40 parts of maize pulp, 4 parts of coronatine, 1.5 parts of trehalose, shitosan
2 parts, 7.2 parts of proline, 3.8 parts of epiphysin, material water weight ratio be 1: 1.2；The raw material is prepared by mixing into pedigree seed culture medium,
Aseptically, one-level parent species are accessed, every one-level parent species are transferred 6 bottles of original seeds, through cultivating 5 days in 22 DEG C of constant temperature dark, so
Fluctuation temperature culture afterwards, until covering with bottle is two grades of kinds；Described Fluctuation temperature culture is by dark culturing temperature from 2 DEG C of h-1It is incremented to 44
DEG C, and 75min is maintained at 44 DEG C, 22 DEG C of renewal cultivations are replaced in after the completion for the treatment of.
3. cultigen is cultivated：Raw material is chosen by weight：Lactobacillus plantarum 0.5-1.5 parts, calcium gluconae 2-5 parts,
Oxalic acid 1-3 parts, gypsum 3-9 parts, glycine betaine 1.5-2.5 parts, cotton seed hulls 8-10 parts, AMS 0.5-1 parts, expect that water weight ratio is
1: 1.2, the raw material is prepared by mixing into Cultivar culture medium, two grades of kinds are aseptically accessed, every two grades of parent species turn
6 bottles of cultigens are connect, through culture under 27.5 DEG C of steady temperatures and illumination until covering with bottle as cultigen；Described stationary temperature
28 DEG C and 4000lux are respectively with illumination.
Test example
The mycelial growth rate determination test of wild-type champignon cultigen under hot environment
Choose the wild-type champignon cultigen of tradition culture as a control group, the wild-type champignon of the culture of the embodiment of the present invention 3 is planted
Cultivate as test group, the cultigen bacterial strain of control group and test group is inoculated on PDA plate culture medium respectively respectively, 3 times
Repeat, inoculum concentration is identical to cultivate while being placed in 40 DEG C of environment, and incubation time is 10 days, and determines mycelial growth rate.It is described
Mycelial growth rate assay method should be technology well-known to those skilled in the art.
Mycelial growth rate (cm/d)=colony radius (cm)/mycelial growth number of days (d)
The measurement result of two groups of bacterial strain mycelial growth rates is shown in Table 1
Two groups of mycelial growth rates under the hot conditions of table 1
By table 1 it will be apparent that, with tradition culture wild-type champignon cultigen bacterial strain compared with, the present invention domestication culture it is wild
Cultivating champignon kind (40 DEG C) hair bacterium in hot environment are good, and mycelial growth rate does not slow down, heat-resist, it may thus be appreciated that
Under hot conditions, the wild-type champignon cultigen of cultivation present invention domestication is obtained in that high yield, so as to break the production of mushroom
Season.
Claims (5)
1. a kind of high temperature resistant wild-type champignon strain artificial acclimation method, it is characterised in that：Including step in detail below：
1. parent species are cultivated：Collection nature field grown 2500-2800 meter Loess Plateau of Gansu Province's height above sea level a big lid thickness,
Handle is short, the wild-type champignon of 80to 90mature and non-parachute-opening as kind of a mushroom, parent species are then made using tissue isolation, by weight
Choose raw material：Eucalyptus considers 15-30 parts, Chinese chestnut bud husk 10-20 parts, glucose 5-10 parts, vitamin B2 1.5-4.5 parts, vitamin to be worth doing
B62.5-4.5 parts, -4 parts of vitamin E2, vitamin C 1-3 parts, urea 3-6 parts, corn bran 6-11 parts, bone meal 7-12 parts, horse
5-7 parts of bell potato waste residue, bean dregs 12-15 parts, jasmonic 0.5-1.5 parts, material water weight ratio be 1:1.2；The raw material is mixed and is made
It is standby to be then aseptically inoculated with wild-type champignon parent species into slant medium, first cultivated 5 days through under 25 DEG C of constant temperature, Ran Houre
Swash culture 1-1.5 hour, then ultraviolet treatment 30-60min is replaced in 25 DEG C of renewal cultivations after the completion for the treatment of, cultivate to covering with
Inclined-plane；
2. original seed is cultivated：Raw material is chosen by weight：Maize pulp 30-50 parts, coronatine 3-5 parts, trehalose 1-2 parts, shell gather
It is sugared 1.5-2.5 parts, proline-4 .5-9.8 parts, epiphysin 2.4-5.2 parts, material water weight ratio be 1: 1.2；The raw material is mixed
Pedigree seed culture medium is prepared into, aseptically, one-level parent species, every one-level parent species 5-7 bottles of original seed of switching, through 22 DEG C of perseverances is accessed
5 days are cultivated in warm dark, then Fluctuation temperature culture, until covering with bottle is two grades of kinds；
3. cultigen is cultivated：Raw material is chosen by weight：Lactobacillus plantarum 0.5-1.5 parts, calcium gluconae 2-5 parts, oxalic acid
1-3 parts, gypsum 3-9 parts, glycine betaine 1.5-2.5 parts, cotton seed hulls 8-10 parts, AMS 0.5-1 parts, material water weight ratio is 1:
1.2, the raw material is prepared by mixing into Cultivar culture medium, aseptically access two grades of kinds, every two grades of parent species switching
5-7 bottles of cultigen, through culture under 25-30 DEG C of steady temperature and illumination until covering with bottle as cultigen.
2. a kind of high temperature resistant wild-type champignon strain artificial acclimation method according to claim 1, it is characterised in that：Step is 1.
The temperature range of described heat shock culture is 47-50 DEG C.
3. a kind of high temperature resistant wild-type champignon strain artificial acclimation method according to claim 1, it is characterised in that：Step is 1.
Cultivation temperature constant range is 30-32 DEG C during described ultraviolet treatment.
4. a kind of high temperature resistant wild-type champignon strain artificial acclimation method according to claim 1, it is characterised in that：Step is 2.
Dark culturing temperature is incremented to 44 DEG C by described Fluctuation temperature culture from 28 DEG C with 2 DEG C/h, and maintains 1-1.5h at 44 DEG C, has been processed
22 DEG C of renewal cultivations are replaced in after.
5. a kind of high temperature resistant wild-type champignon strain artificial acclimation method according to claim 1, it is characterised in that：Described
3. middle stationary temperature and illumination are respectively 28 DEG C and 4000lux to step.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168992A (en) * | 2018-10-27 | 2019-01-11 | 太湖县金江源农业发展有限公司 | 238 mycelia of mushroom is improved in the method for cultivating seeds of bacteria phase heat resisting temperature |
| CN109601247A (en) * | 2019-02-12 | 2019-04-12 | 陕西金西生物科技发展有限公司 | A kind of high temperature resistant mushroom culture medium formula and its cultural method |
| CN109661972A (en) * | 2019-02-12 | 2019-04-23 | 陕西金西生物科技发展有限公司 | A kind of selection of high temperature resistant mushroom |
| CN111418443A (en) * | 2020-05-08 | 2020-07-17 | 青海大学 | Application of salicylic acid or proline in high-temperature cultivation of lentinus edodes |
| CN112544341A (en) * | 2020-12-15 | 2021-03-26 | 贵州丰源现代农业有限公司 | Rapid propagation method and application of winter edible fungus |
-
2017
- 2017-03-06 CN CN201710127345.7A patent/CN106929463A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168992A (en) * | 2018-10-27 | 2019-01-11 | 太湖县金江源农业发展有限公司 | 238 mycelia of mushroom is improved in the method for cultivating seeds of bacteria phase heat resisting temperature |
| CN109601247A (en) * | 2019-02-12 | 2019-04-12 | 陕西金西生物科技发展有限公司 | A kind of high temperature resistant mushroom culture medium formula and its cultural method |
| CN109661972A (en) * | 2019-02-12 | 2019-04-23 | 陕西金西生物科技发展有限公司 | A kind of selection of high temperature resistant mushroom |
| CN109661972B (en) * | 2019-02-12 | 2022-03-04 | 陕西金西生物科技发展有限公司 | Breeding method of high-temperature-resistant shiitake mushrooms |
| CN111418443A (en) * | 2020-05-08 | 2020-07-17 | 青海大学 | Application of salicylic acid or proline in high-temperature cultivation of lentinus edodes |
| CN111418443B (en) * | 2020-05-08 | 2021-11-23 | 青海大学 | Application of proline in high-temperature cultivation of lentinus edodes |
| CN112544341A (en) * | 2020-12-15 | 2021-03-26 | 贵州丰源现代农业有限公司 | Rapid propagation method and application of winter edible fungus |
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Application publication date: 20170707 |