CN103650920A - Method for fermenting ganoderma lucidum mycelia liquid rich in microelements - Google Patents

Method for fermenting ganoderma lucidum mycelia liquid rich in microelements Download PDF

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CN103650920A
CN103650920A CN201310728840.5A CN201310728840A CN103650920A CN 103650920 A CN103650920 A CN 103650920A CN 201310728840 A CN201310728840 A CN 201310728840A CN 103650920 A CN103650920 A CN 103650920A
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liquid
ganoderma lucidum
fermentation
micro
mycelia
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凌中鑫
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Abstract

The invention discloses a method for fermenting ganoderma lucidum mycelia liquid rich in microelements. The method comprises the first step of transplanting strains onto a slant culture medium for cultivation and activation to obtain slant strains, the second step of making the slant strains into a spore suspension, inoculating a liquid culture medium with the spore suspension and performing shake-flask culture to obtain liquid strains, the third step of performing high-temperature sterilization on a fermentation medium, cooling the sterilized fermentation medium, then inoculating the fermentation medium with the liquid strains and performing shake-flask culture to obtain fermentation broth containing mycelia, and the fourth step of separating the mycelia from the fermentation broth in a filtered mode, washing the mycelia with pure water, washing off the culture medium, collecting the mycelia and drying the mycelia to obtain dry mycelia. The method is characterized in that the microelements are added into the fermentation medium. According to the method, the content of microelements in the ganoderma lucidum mycelia can be increased. Besides, the method is easy to operate, low in cost and applicable to industrial production.

Description

The method of the micro-ganoderma lucidum mycelium liquid fermentation of a kind of richness
Technical field
The present invention relates to fungi fermentation engineering, in particular to the micro-liquid fermentation of Absorption of Ganoderma lucidum, produce mycelial production method.
Background technology
Glossy ganoderma claims again polyporus lucidus, refreshing sesame, sesame grass, celestial grass, auspicious grass, is the complete stool of the red sesame of Polyporaceae plant or purple sesame.Take Ganoderma Sinense drug effect as best, glossy ganoderma originates in east Asia, distribution in China the widest in Jiangxi, glossy ganoderma is as the China's tradition valuable ingredient of traditional Chinese medicine that has thousands of years medicinal histories, possesses very high medical value, modern pharmacology through scientific research institution many decades studies confirm that, glossy ganoderma, for strengthening body immunity, regulates blood sugar, control blood pressure, auxiliary tumor chemoradiotherapy, liver protecting, promotes the aspects such as sleep all to have significant curative effect.
People have carried out a large amount of research to the pharmacological action of glossy ganoderma, confirm that it has pharmacological activity and clinical effect widely.Through a large amount of studies have shown that, the mycelium that Ganoderma lucidum submerged fermentation is produced and the composition fairly similar of natural glossy ganoderma, and proved that it has similar pharmacological action and clinical effectiveness to natural glossy ganoderma,
Glossy ganoderma chemical composition mainly contains ergosterol 0.3%~0.4%, fungal lysozyme and acid protease, L-mannitol, Xi alcohol.In water-soluble extracting liquid, contain water soluble protein, several amino acids, polypeptide and the polysaccharoses such as asparatate, glutamic acid, arginine, lysine, leucine, alanine, tryptophan, threonine, proline, methionine, phenyl alanine, serine.Still resinous, lactone, coumarin etc.
Purple sesame is containing ergosterol, and organic acid is ricinic acid, fumaric acid.In addition, and containing amino acid, glucose, polysaccharose, resin and mannitol.General glossy ganoderma has the reactions such as alkaloid, sterol, lactone, coumarin, acidic resins, amino acid, grease, reducing substances.
The chemical composition of Ganoderma is comparatively complicated, and because of bacterial classification used, the bacterial classification place of production, cultivation method, extraction process, formulation method different different.In the fruit body of Ganoderma, mycelium and spore, contain polysaccharose, the sweet class of core, furan derivative, steroid ferment class, alkaloids, protein, polypeptide, amino acids, triterpenes, sequiterpene, organic germanium, mineral salt etc.GL-B is one of main active ingredient of glossy ganoderma, has antitumor, immunological regulation, hypoglycemic, anti-oxidant, reducing blood lipid and anti-aging effects.Not lower over one hundred kind of the contained triterpenes of glossy ganoderma, wherein take tetracyclic triterpenes as main, and the bitter taste of glossy ganoderma is relevant with contained triterpenes.Triterpenes is also one of active ingredient of glossy ganoderma, and human liver cancer cell is had to cytotoxicity, also can suppress the release of histamine, has hepatoprotective effect and has anti-allergic effects etc.
Human total weight's ten thousand/following element, as zinc, copper, manganese, chromium, selenium, molybdenum, cobalt, fluorine etc., is called trace element.The content of trace element in human body is really very little, as zinc only accounts for human total weight's 1,000,000/33.
Up to the present, being identified the essential trace element relevant with health and life has 18 kinds, has iron, copper, zinc, cobalt, manganese, chromium, selenium, iodine, nickel, fluorine, molybdenum, vanadium, tin, silicon, strontium, boron, rubidium, arsenic etc.This every kind trace element has its special physiological function.Although they are minimum at people's in-vivo content, they are very necessary to some conclusive metabolism that maintain in human body.Once lacked these essential trace elements, human body just there will be disease, even threat to life.
It is imperative that thereby the improvement by process increases substantially the content of trace element in ganoderma lucidum mycelium.
Summary of the invention
The object of the present invention is to provide the method for the micro-ganoderma lucidum mycelium liquid fermentation of a kind of richness, to overcome the low defect of micronutrient levels in prior art ganoderma lucidum mycelium.
After systematic research and test, proposed to improve by adding trace element the production method of the output of Ganoderma lucidum submerged fermentation Chinese caterpillar fungus hypha body weight trace element, method comprises:
(1) bacterial strain is transferred and on slant medium, cultivate, activate, obtain slant strains;
(2) slant strains is made to spore suspension, be connected in liquid nutrient medium, shaking flask is cultivated, and obtains liquid spawn;
(3) cooling after fermentation medium high-temperature sterilization, then by liquid spawn access, shaking flask is cultivated, and obtains containing mycelial zymotic fluid;
(4) by filtering mycelium and separation of fermentative broth, purified rinse water mycelia, washes away medium, collects mycelium, dries, and makes dry mycelium.
In the second step of described method, liquid spawn culture medium formula used is micro-0.05%-0.5%, peptone 1.5%, sucrose 3%, KH 2pO 40.1-0.2%, water 1000mL, pH7.5-8.
In the second step of described method, process conditions used are: liquid spawn culture medium liquid amount is 60mL/250mL triangular flask; Liquid spawn is cultivated 4d under 24 ℃, rotating speed 160r/min.
In the 3rd step of described method, culture medium prescription used is micro-0.1%-1.6%, and egg is from peptone 1.5%, sucrose 3%, KH 2pO 40.1-0.2%, water 1000mL, pH7.5-8.
In the 3rd step of described method, process conditions used are: liquid amount 200mL/500mL triangular flask, and adopt 6% inoculum concentration that seed liquor is accessed in fermentation shake flask, at 23 ℃, shake 150r/min rotating speed and cultivate 10d.
Described trace element and ratio thereof are that the ratio of selenium element, chromium element, zinc element, manganese element and boron element is: 10: 10: 3: 3: 1.Key technology of the present invention is in fermentation medium, adds selenium element, chromium element, zinc element, manganese element and boron element simultaneously.
The present invention has improved the content of trace element in ganoderma lucidum mycelium, and then improves the drug effect of ganoderma lucidum mycelium, and significant, the mycelia of production can be used as functional food, health products, the raw material of medicine; This method is easy to operate, and cost is low, is suitable for industrial production.
Embodiment
Embodiment 1: bacterial strain is transferred and on PDA slant medium, at 25 ℃, cultivated 7d, activation 2 times, obtain slant strains; Scrape gently and wash slant strains and it is made to spore suspension, then use sterile gauze (6 layers) to filter out the mycelia in suspension, the moderate Reishi sporule of every bottle of liquid spawn access concentration, the mycelium pellet in the liquid that makes to make is tiny and even, zymotic fluid is clear disappear transparent.Liquid spawn culture medium formula is: micro-0.05%-0.5%, peptone 1.5%, sucrose 3%, KH 2pO 40.1-0.2%, water 1000mL, pH7.5-8, liquid spawn culture medium liquid amount is 60mL/250mL triangular flask; Liquid spawn is cultivated 4d under 24 ℃, rotating speed 160r/min, obtains liquid spawn.
Use optimized fermentation medium: micro-0.1%-1.6%, peptone 1.5%, sucrose 3%, KH2PO40.1-0.2%, water 1000mL, pH7.5-8.Process conditions used are: liquid amount 200rmL/500mL triangular flask, and adopt 6% inoculum concentration that seed liquor is accessed in fermentation shake flask, at 23 ℃, shake 150r/min rotating speed and cultivate 10d.Obtain the zymotic fluid that most of mycelium morphology is mycelium pellet; After filtration by mycelium and separation of fermentative broth, then use purified rinse water mycelia, wash away after medium, collect mycelium, dry.

Claims (6)

1. a method for the micro-ganoderma lucidum mycelium liquid fermentation of richness, is characterized in that: the method comprises following four steps: (1) is transferred bacterial strain and cultivated on slant medium, activates, and obtains slant strains; (2) slant strains is made to spore suspension, be connected in liquid nutrient medium, shaking flask is cultivated, and obtains liquid spawn; (3) cooling after fermentation medium high-temperature sterilization, then by liquid spawn access, shaking flask is cultivated, and obtains containing mycelial zymotic fluid; (4) by filtering mycelium and separation of fermentative broth, purified rinse water mycelia, washes away medium, collects mycelium, dries, and makes dry mycelium.Key problem in technology is in fermentation medium, to add trace element.This method can improve the content of trace element in ganoderma lucidum mycelium.
2. according to the method for the micro-ganoderma lucidum mycelium liquid fermentation of richness claimed in claim 1, it is characterized in that: in the second step of described method, liquid spawn culture medium formula used is: micro-0.05%-0.5%, peptone 1.5%, sucrose 3%, KH 2pO 40.1-0.2%, water 1000mL, pH7.5-8.
3. according to the method for the micro-ganoderma lucidum mycelium liquid fermentation of richness claimed in claim 1, it is characterized in that: in the second step of described method, process conditions used are: liquid spawn culture medium liquid amount is 60mL/250mL triangular flask; Liquid spawn is cultivated 4d under 24 ℃, rotating speed 160r/min.
4. according to the method for the micro-ganoderma lucidum mycelium liquid fermentation of richness claimed in claim 1, it is characterized in that: in the 3rd step of described method, culture medium prescription used is micro-0.1%-1.6%, peptone 1.5%, sucrose 3%, KH 2pO 40.1-0.2%, water 1000mL, pH7.5-8.
5. according to the method for the micro-ganoderma lucidum mycelium liquid fermentation of richness claimed in claim 1, it is characterized in that: in the 3rd step of described method, process conditions used are: liquid amount 200mL/500mL triangular flask, adopt 6% inoculum concentration that seed liquor is accessed in fermentation shake flask, at 23 ℃, shake 150r/min rotating speed and cultivate 10d.
6. according to the method for the micro-ganoderma lucidum mycelium liquid fermentation of richness claimed in claim 1, it is characterized in that: described trace element and ratio thereof are that the ratio of selenium element, chromium element, zinc element, manganese element and boron element is: 10: 10: 3: 3: 1.
CN201310728840.5A 2013-12-20 2013-12-20 Method for fermenting ganoderma lucidum mycelia liquid rich in microelements Pending CN103650920A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104350953A (en) * 2014-11-19 2015-02-18 李志芳 Cultivation method for ganoderma lucidum
CN110214626A (en) * 2019-07-16 2019-09-10 灌南县人民政府蔬菜办公室 A kind of straw mushroom cultural method

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104350953A (en) * 2014-11-19 2015-02-18 李志芳 Cultivation method for ganoderma lucidum
CN104350953B (en) * 2014-11-19 2016-08-31 李志芳 A kind of breeding method of blood Ganoderma
CN110214626A (en) * 2019-07-16 2019-09-10 灌南县人民政府蔬菜办公室 A kind of straw mushroom cultural method
CN110214626B (en) * 2019-07-16 2021-08-10 灌南县人民政府蔬菜办公室 Straw mushroom culture method

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Application publication date: 20140326