CN101481658A - Novel production process of Ganoderma lucidum mycelium powder - Google Patents
Novel production process of Ganoderma lucidum mycelium powder Download PDFInfo
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- CN101481658A CN101481658A CNA2008101678098A CN200810167809A CN101481658A CN 101481658 A CN101481658 A CN 101481658A CN A2008101678098 A CNA2008101678098 A CN A2008101678098A CN 200810167809 A CN200810167809 A CN 200810167809A CN 101481658 A CN101481658 A CN 101481658A
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Abstract
The invention discloses a novel production technique of ganoderma lucidum mycelium powder, which combines the respective advantages of liquid fermentation and solid fermentation, and is characterized by safety, sanitation, low cost, high efficiency and environmental protection. Through the production technique, the ganoderma lucidum mycelium powder can be effectively extracted, with mycelium dry basis yield more than or equal to 25% and mycelium powder polysaccharide content more than or equal to 8%. The invention not only reserves the effective activity of the ganoderma lucidum mycelium powder, which is the same as wild ganoderma lucidum, but also has continuous and simple production process flow.
Description
Technical field
This patent relates to a kind of novel production process of Ganoderma lucidum mycelium powder, particularly a kind of technology of solid-liquid combination fermentative Production Ganoderma lucidum mycelium powder.
Background technology
Glossy ganoderma (Ganoderma lucidum) has another name called Ganoderma lucidum seu Japonicum, Ganoderma, Ganoderma Lucidum, is Basidiomycetes polyporaceae Ganoderma.Since ancient times, just that it is edible as medicine or tonic the East Asia Region people; Now, glossy ganoderma dietotherapeutic, the Pharmacopoeia of the People's Republic of China (2000 editions, 1 one) have recorded glossy ganoderma (red sesame and purple sesame sporophore) as legal Chinese medicinal materials; Obtain Ministry of Health's approval in October, 2003 as new resource food; " U.S.'s herbal medicine pharmacopeia and treatment summary " also recorded glossy ganoderma.
Aspects such as modern medicine study finds that main effective constituent is ganoderan in the glossy ganoderma, and its nourishing function mainly concentrates on and regulate immune, antitumor, hypotensive, reducing blood-fat, protects liver, antiviral.When glossy ganoderma medicine price cheating value was extensively approved, glossy ganoderma or ganoderan security of products, annual production, cost were the bottlenecks that use in restriction glossy ganoderma market.
At present, because wild natural glossy ganoderma resource-constrained; Simultaneously the artificial culture glossy ganoderma obtains Ganoderma sporophore, though ganoderan can be extracted, the pollution and the pesticide residue that the glossy ganoderma substratum can't be brought are got rid of, and the earning rate of polysaccharide also very low, have only 8-15%.The polysaccharide content of Ganoderma mycelium will be apparently higher than the polysaccharide content of sporophore, and mycelial cell walls is loose than the sporophore cell wall structure, and the ganoderan in the cell is easier to be extracted.Fermentation method can obtain a large amount of Ganoderma myceliums at short notice by regulating substratum composition, culture temperature and time etc., and these mycelial chemical constitution or physiological functions show that by analysis to Ganoderma sporophore that agricultural cultivation obtains be very similar.Mainly take zymotechnique both at home and abroad, obtain Ganoderma mycelium, overcome pollution and pesticide residue, two kinds of fermentation process are arranged: a kind of is to adopt the bacterial classification liquid fermentation technology to obtain Ganoderma mycelium; Another kind is to adopt solid-fermented technique to obtain Ganoderma mycelium.First kind the liquid fermentation technology facility investment is big, floor space is big, and particularly water consumption is quite big, and the earning rate of polysaccharide is not high yet, the highest generally can reach about 45%, general can only reach about 20%, and it is big to draw active ingredient loss, and the turnout of polysaccharide is low.Though second method solid-fermented technique income height, microbiological contamination easily, the strictness of bacterial classification purification condition, and also industrialization is grown, is difficult to fermentation period.
Summary of the invention
The production technique that the purpose of this invention is to provide a kind of Ganoderma lucidum mycelium powder, it combines the advantage separately of liquid fermenting and solid fermentation, utilize grain crop residue to be raw material, the technological process of production is continuous, simple, and heavy metal, bacteria content meet the state food standard, and pollution-free in the production process, it is the method for a kind of safety and sanitation, with low cost, high-efficiency environment friendly, extract Ganoderma lucidum mycelium powder, complete its effective active that kept, consistent with the wild Ganoderma effect.Mycelia butt yield 〉=25% wherein, mycelia polysaccharide content 〉=8%, i.e. 100kg substratum (dry weight), but the above Ganoderma lucidum mycelium of 25g, ganoderma polyoses content in the mycelia 〉=8%.
Production technique provided by the present invention is to implement like this:
1. substratum is prepared.
The solid medium preparation: grain crop konjaku slag and manioc waste weight ratio are that 1:1 pulverizes mixing, add water then, and amount of water is 2/3rds of a grain crop dry weight.Inoculum size is 10% of a grain crop dry weight, cultivates 12-16 days for 25-30 ℃.Sterilising conditions is: 121 ℃, and 1 hour.
PDA slant medium: PDA solid (potato extracting solution 1.0L, sucrose 20.0g, KH
2PO
43.0g, MgSO
4.7H
2O1.5g, peptone 1.0g, agar 15.0g, pH7.0) sterilising conditions is: 121 ℃, 30min;
Liquid seed culture medium: PDA liquid (potato extracting solution 1.0L, sucrose 20.0g, KH
2PO
43.0g, MgSO
47H
2O 1.5g, peptone 1.0g, pH7.0), sterilising conditions is: 121 ℃, 30min.
2. actication of culture.
Ganoderma lucidum seed Ganoderma lucidum bacterial classification inoculation to the PDA slant medium, was cultivated 3-4 days activation for 25-30 ℃.The bacterial classification that activation is good is got 1cm
2The lawn 3-4 piece of size is inoculated in the liquid seed culture medium (500mL triangular flask, liquid amount are 200mL), and 25-30 ℃, cultivated 4-5 days for 150 rev/mins, obtain first order seed.First order seed by 10% inoculum size is inoculated in the seed liquor substratum (500mL triangular flask, liquid amount are 200mL), 25-30 ℃, cultivates 3-4 days, and obtains secondary seed for 150 rev/mins.
3. fermentation.
Secondary seed is inoculated bottled solid medium, mix thoroughly, cultivated about 12-16 days down, in bag, cover with till the mycelia of white for 25-30 ℃.
4. oven dry.
After the fermentation ends, mycelia and substratum are dried in 50 ℃ baking oven together, moisture requirement is below 5%.
5. pulverize.
The mycelia of oven dry is pulverized, require less than 80 orders.
6. sterilization, bottling.
Description of drawings:
The concise and to the point flow process of production technique is as follows: as Fig. 1
Below in conjunction with embodiment, the present invention is further detailed.
Embodiment
Embodiment 1:
1. substratum is prepared.
The solid medium preparation: grain crop konjaku slag 100kg and Semen Maydis grit 100kg pulverize and mix, and add water 134L mixing then.High-temperature sterilization, sterilising conditions is: 121 ℃, 1h.
Slant medium preparation: PDA solid (potato extracting solution 1.0L, sucrose 20.0g, KH
2PO
43.0g, MgSO
47H
2O 1.5g, peptone 1.0g, agar 15.0g, pH7.0).High-temperature sterilization, sterilising conditions is: 121 ℃, 30min;
Liquid seed culture medium: PDA liquid (potato extracting solution 1.0L, sucrose 20.0g, KH
2PO
43.0g, MgSO
47H
2O 1.5g, 1.0 g of peptone, pH7.0).High-temperature sterilization, sterilising conditions is: 121 ℃, 30min;
2. actication of culture.
Ganoderma lucidum seed Ganoderma lucidum bacterial classification inoculation to the PDA slant medium, is cultivated 4d for 28 ℃.
4 of the lawns that the bacterial classification that activation is good is got about 1cm2 size are inoculated in the seed liquor substratum (500mL triangular flask, liquid amount are 200mL), and 28 ℃, 150r/min cultivates 5d, obtains first order seed.The 20g first order seed is inoculated in the seed liquor substratum (500mL triangular flask, liquid amount are 200mL), and 28 ℃, 150r/min cultivates 7d, obtains secondary seed.
3. get secondary seed 20g and inoculate in the packed 200g solid medium, mix thoroughly, cultivated 12 days down for 28 ℃, cover with the mycelia of white in the bag.
4. after the fermentation ends, mycelia and substratum are dried in 50 ℃ baking oven together, up to moisture below 5%.
5. the mycelia of oven dry is pulverized, the pulverized particles index is less than 80 orders.
6. sterilization, pack.
Embodiment 2:
1. substratum is prepared.
The solid medium preparation: grain crop sweet potato waste 100kg and Semen Maydis grit 100kg pulverize and mix, and add water 134L mixing then.High-temperature sterilization, sterilising conditions is: 121 ℃, 1h.
Slant medium preparation: PDA solid (potato extracting solution 1.0L, sucrose 20.0g, KH
2PO
43.0g, MgSO
47H
2O 1.5g, peptone 1.0g, agar 15.0g, pH7.0).High-temperature sterilization, sterilising conditions is: 121 ℃, 30min;
Liquid seed culture medium: PDA liquid (potato extracting solution 1.0L, sucrose 20.0g, KH
2PO
43.0g, MgSO
47H
2O 1.5g, peptone 1.0g, pH7.0).High-temperature sterilization, sterilising conditions is: 121 ℃, 30min;
2. actication of culture.
Ganoderma lucidum seed Ganoderma lucidum bacterial classification inoculation to the PDA slant medium, is cultivated 3d for 28 ℃.
The bacterial classification that activation is good is got about 1cm
23 of the lawns of size are inoculated in the seed liquor substratum (500mL triangular flask, liquid amount are 100mL), and 28 ℃, 150r/min cultivates 4d, obtains first order seed.The 10g first order seed is inoculated in the seed liquor substratum (500mL triangular flask, liquid amount are 100mL), and 28 ℃, 150r/min cultivates 5d, obtains secondary seed.
3. get secondary seed 10g and inoculate in the packed 100g solid medium, mix thoroughly, cultivated 13 days down for 28 ℃, cover with the mycelia of white in the bag.
4. after the fermentation ends, mycelia and substratum are dried in 50 ℃ baking oven together, up to moisture below 5%.
5. the mycelia of oven dry is pulverized, the pulverized particles index is less than 80 orders.
6. sterilization, pack.
Claims (5)
1, the solid medium of a kind of grain crop is characterized in that: grain crop konjaku slag and manioc waste weight ratio are that 1:1 pulverizes mixing, add water then, and amount of water is 2/3rds of a grain crop dry weight.Inoculum size is 10% of a grain crop dry weight, cultivates 12-16 days for 25-30 ℃.Sterilising conditions is: 121 ℃, and 1 hour.
2, a kind of novel Ganoderma mycelium production technique, it is characterized in that: this processing step is as follows:
A. Ganderma lucidum strain Ganoderma lucidum is inoculated on the PDA slant medium, cultivated 3-4 days activation for 25-30 ℃;
B. the bacterial classification that activation is good is got 1cm
2The lawn 3-4 piece of size is inoculated in the liquid seed culture medium (500mL triangular flask, liquid amount are 200mL), and 25-30 ℃, cultivated 4-5 days for 150 rev/mins, obtain first order seed;
C. 600mL (3 bottles) primary seed solution is inoculated in the liquid seed culture medium (500L fermentor tank, liquid amount are 300L), 25-30 ℃, cultivated 3-4 days, and obtained secondary seed for 150 rev/mins;
D. secondary seed is inoculated bottled solid medium, mix thoroughly, cultivated about 12-16 days down for 25-30 ℃, in bag, cover with till the mycelia of white;
E. with the mycelium oven dry, pulverize.
3, according to the described PDA slant medium of a step in the claim 2, it is characterized in that: PDA solid (potato extracting solution 1.0L, sucrose 20.0g, KH
2PO
43.0g, MgSO
4.7H
2O 1.5g, peptone 1.0g, agar 15.0g, pH7.0) sterilising conditions is: 121 ℃, 30min.
4,, be Ganoderma lucidum seed Ganoderma lucidum according to the described Ganderma lucidum strain of a step in the claim 2.
5, according to the described liquid seed culture medium of b step in the claim 2, it is characterized in that: PDA liquid (potato extracting solution 1.0L, sucrose 20.0g, KH
2PO
43.0g, MgSO
4.7H
2O 1.5g, peptone 1.0g, pH7.0), sterilising conditions is: 121 ℃, 30min.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103238464A (en) * | 2013-05-17 | 2013-08-14 | 绍兴儒林生物科技有限公司 | Method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation |
| CN103393095A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Processing method of compound hypha powder |
| CN103393094A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Preparation method of ganoderma lucidum mycelium slices |
| CN103584074A (en) * | 2013-11-17 | 2014-02-19 | 哈尔滨艾克尔食品科技有限公司 | Ganoderma lucidum chewable tablet |
| CN103689550A (en) * | 2012-09-27 | 2014-04-02 | 江苏大学 | Ganoderma lucidum functional food and preparation method thereof |
| CN103933081A (en) * | 2014-04-23 | 2014-07-23 | 浙江瑞新药业股份有限公司 | Preparation method for ganoderma capense medicines |
| CN103966107A (en) * | 2014-05-13 | 2014-08-06 | 大连佳腾生物科技有限公司 | Culture medium and method for producing multi-component high-activity ganoderma lucidum powder by using same |
| CN104106372A (en) * | 2014-06-23 | 2014-10-22 | 黄艳芳 | Lucid ganoderma mycelium and method for preparing lucid ganoderma mycelium body |
| CN105018350A (en) * | 2014-04-25 | 2015-11-04 | 江苏中祥高科技实业有限公司 | Method for producing high ganoderma triterpenes content ganoderma lucidum mycelium |
| CN105851746A (en) * | 2016-03-31 | 2016-08-17 | 浙江海洋学院 | Preparation method of thick-shell mytilus edulis glycosaminoglycan drink |
| CN107177515A (en) * | 2017-07-21 | 2017-09-19 | 宁德师范学院 | A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation |
| CN107177510A (en) * | 2016-03-10 | 2017-09-19 | 王大健 | A kind of fontanesia leaf shelf fungus and its purposes in medicine is prepared |
| CN107586727A (en) * | 2017-10-26 | 2018-01-16 | 湖南和平生物科技有限公司 | A kind of fermentation process of ganoderma lucidum mycelium |
| CN109234171A (en) * | 2018-09-06 | 2019-01-18 | 南昌大学 | A kind of preparation method of Se-rich lucid ganoderma hypha powder |
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2008
- 2008-10-13 CN CNA2008101678098A patent/CN101481658A/en active Pending
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103689550A (en) * | 2012-09-27 | 2014-04-02 | 江苏大学 | Ganoderma lucidum functional food and preparation method thereof |
| CN103238464B (en) * | 2013-05-17 | 2014-10-08 | 绍兴儒林生物科技有限公司 | Method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation |
| CN103238464A (en) * | 2013-05-17 | 2013-08-14 | 绍兴儒林生物科技有限公司 | Method of utilizing dendrobium candidum slag for cultivating ganoderma through solid fermentation |
| CN103393095A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Processing method of compound hypha powder |
| CN103393094B (en) * | 2013-07-19 | 2014-07-09 | 乳山市华隆生物科技有限公司 | Preparation method of ganoderma lucidum mycelium slices |
| CN103393094A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Preparation method of ganoderma lucidum mycelium slices |
| CN103584074A (en) * | 2013-11-17 | 2014-02-19 | 哈尔滨艾克尔食品科技有限公司 | Ganoderma lucidum chewable tablet |
| CN103933081A (en) * | 2014-04-23 | 2014-07-23 | 浙江瑞新药业股份有限公司 | Preparation method for ganoderma capense medicines |
| CN105018350A (en) * | 2014-04-25 | 2015-11-04 | 江苏中祥高科技实业有限公司 | Method for producing high ganoderma triterpenes content ganoderma lucidum mycelium |
| CN103966107A (en) * | 2014-05-13 | 2014-08-06 | 大连佳腾生物科技有限公司 | Culture medium and method for producing multi-component high-activity ganoderma lucidum powder by using same |
| CN103966107B (en) * | 2014-05-13 | 2016-08-24 | 大连佳腾生物科技有限公司 | A kind of culture medium and by this culture medium production multicomponent high activity ganoderma lucidum powder method |
| CN104106372A (en) * | 2014-06-23 | 2014-10-22 | 黄艳芳 | Lucid ganoderma mycelium and method for preparing lucid ganoderma mycelium body |
| CN107177510A (en) * | 2016-03-10 | 2017-09-19 | 王大健 | A kind of fontanesia leaf shelf fungus and its purposes in medicine is prepared |
| CN105851746A (en) * | 2016-03-31 | 2016-08-17 | 浙江海洋学院 | Preparation method of thick-shell mytilus edulis glycosaminoglycan drink |
| CN107177515A (en) * | 2017-07-21 | 2017-09-19 | 宁德师范学院 | A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation |
| CN107586727A (en) * | 2017-10-26 | 2018-01-16 | 湖南和平生物科技有限公司 | A kind of fermentation process of ganoderma lucidum mycelium |
| CN107586727B (en) * | 2017-10-26 | 2020-03-13 | 湖南和平生物科技有限公司 | Fermentation method of ganoderma lucidum mycelia |
| CN109234171B (en) * | 2018-09-06 | 2021-08-20 | 南昌大学 | Preparation method of selenium-rich ganoderma lucidum mycelium powder |
| CN109234171A (en) * | 2018-09-06 | 2019-01-18 | 南昌大学 | A kind of preparation method of Se-rich lucid ganoderma hypha powder |
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