CN103933081A - Preparation method for ganoderma capense medicines - Google Patents
Preparation method for ganoderma capense medicines Download PDFInfo
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- CN103933081A CN103933081A CN201410164920.7A CN201410164920A CN103933081A CN 103933081 A CN103933081 A CN 103933081A CN 201410164920 A CN201410164920 A CN 201410164920A CN 103933081 A CN103933081 A CN 103933081A
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Abstract
The invention discloses a preparation method for ganoderma capense medicines, for preparing ganoderma capense tablets, ganoderma capense bacterial injection agent and ganoderma capense bacterial injection solution by primary culture, enlarging culture and fermentation. The preparation method disclosed by the invention has the following beneficial effects that an integrated effect is realized from culturing ganoderma capense to prepare the ganoderma capense tablets, ganoderma capense bacterial injection agent and ganoderma capense bacterial injection solution, the production efficiency of the ganoderma capense medicines is increased, and the economic benefits are improved; the ganoderma capense tablets are capable of treating neurasthenia and climacteric syndrome; the ganoderma capense bacterial injection agent and the ganoderma capense bacterial injection solution are capable of enhancing immunity; the effective utilization rate of ganoderma capense is high; moreover, ganoderma capense mycelia and the fermentation broth of the ganoderma capense mycelia can be completely utilized, the waste of raw materials is avoided, the production cost is reduced, and the raw materials are completely natural materials such as the ganoderma capense mycelia, good in safety, good in use effect, and are beneficial to popularize.
Description
Technical field
The invention belongs to medical technical field, especially a kind of preparation method of Ganoderma capenseD.A.Reid medicine.
Background technology
At present, along with the quickening of modern society's rhythm of life, people's life stress is increasing, operating pressure is also increasing, therefore, the people of modern society just easily cause dietary structure improper because pressure is excessive, body burden is excessive, easily cause body immunity to decline, easily cause people to produce the diseases such as neurasthenia, and to involutional people just there will be climacteric syndrome, conventionally be all to adopt medicine to treat for these diseases people, and the medicine of general treatment is all made by chemical formulation conventionally, be not to adopt natural material to make, these medicines of making by chemical formulation all have certain side effect conventionally, and the medicine that natural material is prepared into is free from side effects, therefore, it has been very necessary adopting natural material to be prepared into medicine.
Ganoderma capenseD.A.Reid is the one in Ganoderma, it is a kind of natural material, there is raising immunity, treatment neurasthenia, effect for the treatment of climacteric syndrome etc., people are the object that Ganoderma capenseD.A.Reid is directly eaten to reach picked-up medicinal ingredient conventionally, easily make the medicinal ingredient of Ganoderma capenseD.A.Reid be absorbed by the body less and directly eat, most of effective ingredient is difficult for being absorbed, easily cause the Efficiency Decreasing of Ganoderma capenseD.A.Reid medicinal ingredient picked-up, effect is poor, therefore, develop and a kind ofly Ganoderma capenseD.A.Reid is prepared into the method for the medicine that can easily be absorbed by the body and is very necessary.
Summary of the invention
The present invention is directed to the shortcoming of above-mentioned prior art, a kind of preparation method of Ganoderma capenseD.A.Reid medicine is provided, realize Ganoderma capenseD.A.Reid from cultivating to the integrated effect that is prepared into medicine, Ganoderma capenseD.A.Reid mycelium can be prepared into thin sesame sheet, BUZHIJUN injection and BUZHIJUN injection, Ganoderma capenseD.A.Reid mycelium is used completely, utilization rate is high, has reduced production cost, has met people's demand.
The technical solution used in the present invention:
The preparation method of this Ganoderma capenseD.A.Reid medicine, comprises the steps:
The first step, just cultivate, choose 10 parts～20 parts, agar, 10 parts～20 parts of sucrose, 10 parts～15 parts of starch, again the raw material of choosing is encased in seed bottle and forms mixture, then in seed bottle, adding water and supplying mixture capacity is 1 liter, after again mixture being stirred, obtain primary culture medium, then seed bottle is placed to lower exhaust pressure steam sterilizer and carries out sterilization operation, sterilizing time is 30 minutes～40 minutes, after sterilization, treat that seed bottle temperature declines, in the time that seed bottle temperature drops to 24 DEG C～26 DEG C, in seed bottle, accessing Ganoderma capenseD.A.Reid strain cultivates, keeping ambient temperature is 25 DEG C～27 DEG C, treat that Ganoderma capenseD.A.Reid mycelium germination obtains Ganoderma capenseD.A.Reid mycelia,
Second step, amplification culture, choose 10 parts～20 parts, wheat bran, 4 parts～8 parts of Vitamin B6 Tablets, 6 parts～12 parts, magnesium sulfate, 5 parts～10 parts of calcium carbonate, Vitamin B6 Tablets is smashed into pulverulence that can be water-soluble to pieces, again the raw material of choosing is encased in and in vial, forms mixture, then in vial, adding water and supplying mixture capacity is 1 liter, after again mixture being stirred, obtain Secondary media, vial is placed in lower exhaust pressure steam sterilizer and carries out sterilization operation again, sterilizing time is 35 minutes～45 minutes, then the amount of trying to please is the seed tank of 10 liters, getting 5 parts of Secondary media is encased in seed tank again, Secondary media accounts for 50% of seed tank capacity, then be linked into the amplification culture of carrying out Ganoderma capenseD.A.Reid in Secondary media by just cultivating the Ganoderma capenseD.A.Reid mycelia obtaining, keeping cultivation temperature is 25 DEG C～27 DEG C, treat that Ganoderma capenseD.A.Reid mycelia sends out seed tank full and can obtain Ganoderma capenseD.A.Reid mycelium,
The 3rd step, fermentation, will be sent in fermentation tank and ferment after the Ganoderma capenseD.A.Reid mycelium fragmentation obtaining after amplification culture, then in fermentation tank, drop into 5 parts～10 anti crawl agentfroth oil, it is 60%～80% that fermentation tank keeps relative humidity, and fermentation temperature is 30 DEG C～40 DEG C, and fermentation time is 4 days～6 days;
The 4th step, the preparation of thin sesame sheet, Ganoderma capenseD.A.Reid broth extraction after fermentation is completed is in vessel, then the vessel that Ganoderma capenseD.A.Reid fermentation liquid is housed are sent to the evaporation and concentration of carrying out Ganoderma capenseD.A.Reid fermentation liquid in vapourizing furnace, treat that Ganoderma capenseD.A.Reid fermentation liquid forms Ganoderma capenseD.A.Reid extractum, then the Ganoderma capenseD.A.Reid extractum obtaining is sent in baking oven and is dried, oven temperature is 120 DEG C～140 DEG C, after to be dried completing, obtain Ganoderma capenseD.A.Reid solid block, then Ganoderma capenseD.A.Reid solid block is sent to and in pulverizer, is ground into Ganoderma capenseD.A.Reid powder, particle diameter is 80 order～100 orders, again Ganoderma capenseD.A.Reid powder is sent to and in tablet machine, is prepared into thin sesame sheet,
The 5th step, the preparation of Ganoderma capenseD.A.Reid mycelium extracting solution, Ganoderma capenseD.A.Reid mycelium after fermentation is completed is put into and in centrifugal dehydrator, is carried out centrifuge dehydration, the centrifuge dehydration time is 15 minutes～20 minutes, then centrifuge dehydration Ganoderma capenseD.A.Reid mycelium is later sent in baking oven and is dried, oven temperature is 110 DEG C～130 DEG C, after Ganoderma capenseD.A.Reid mycelium is dried, Ganoderma capenseD.A.Reid mycelium is put into and in pulverizer, is ground into Ganoderma capenseD.A.Reid mycelium powder, the particle diameter of Ganoderma capenseD.A.Reid mycelium powder is 40 order～70 orders, again Ganoderma capenseD.A.Reid mycelium powder is put in alcohol extraction pot, then in alcohol extraction pot, add organic solvent, organic solvent and the abundant mix and blend of Ganoderma capenseD.A.Reid mycelium powder are formed to mixture, the ratio of Ganoderma capenseD.A.Reid mycelium powder and organic water solvent is 1:5, then this mixture is put in extractor and extracted, mixture after again extraction being completed is put into and in ether pot, is removed ether or go alcohol operation to obtain Ganoderma capenseD.A.Reid mycelium extracting solution,
The 6th step, the preparation of BUZHIJUN injection, the Ganoderma capenseD.A.Reid mycelium extracting solution and the water for injection that obtain are mixed to get to mixed liquor, the ratio of Ganoderma capenseD.A.Reid mycelium extracting solution and water for injection is 1:500, then by filling machine, this mixed liquor is filled to and in ampoule bottle, is prepared into BUZHIJUN injection;
The 7th step, the preparation of BUZHIJUN injection, the Ganoderma capenseD.A.Reid mycelium extracting solution and the water for injection that obtain are mixed to get to mixed liquor by dilute preparing tank, the ratio of Ganoderma capenseD.A.Reid mycelium extracting solution and water for injection is 1:1000, and then this mixed liquor is washed to embedding interlock unit by plastic soft bottle infusion solutions and carries out embedding and obtain infusion bottle, then by this infusion bottle successively by ultraviolet germicidal lamp sterilization, oven drying, lamp inspection, be packaged to be BUZHIJUN injection.
Further, fermentation tank is mechanical agitation type fermentation tank.
Further, pulverizer is small-sized super micron mill.
Further, tablet machine is Highspeedrotarytabletpress.
Further, organic solvent is the one in ethanol, ether.
Further, filling machine is liquid-filling machine.
Further, anti crawl agentfroth oil is soybean oil.Soybean oil is as anti-crawl agentfoam oil safety non-pollution.
The preparation method of Ganoderma capenseD.A.Reid medicine of the present invention, first Ganoderma capenseD.A.Reid strain is carried out just cultivating, Ganoderma capenseD.A.Reid spawn culture is become Ganoderma capenseD.A.Reid mycelium by amplification culture, just cultivating is in order to allow Ganoderma capenseD.A.Reid strain grow into Ganoderma capenseD.A.Reid mycelia, when after Ganoderma capenseD.A.Reid mycelium germination, because need to drawing a large amount of nutrition, Ganoderma capenseD.A.Reid mycelia can grow, culture medium nutrition in now just cultivating is not enough, therefore, adopt the mode of amplification culture that Ganoderma capenseD.A.Reid mycelia is linked into and in seed tank, carries out amplification culture, allow Ganoderma capenseD.A.Reid mycelia can fully absorb nutrition, allow Ganoderma capenseD.A.Reid can improve speed of production, therefore, making Ganoderma capenseD.A.Reid strain by the mode of second incubation can Fast Growth be Ganoderma capenseD.A.Reid mycelium, speed of production is fast, efficiency is high, save growth time, then the Ganoderma capenseD.A.Reid mycelium obtaining is fermented, in the process of fermentation, the mycelial cell wall fragmentation of Ganoderma capenseD.A.Reid, effective medicinal ingredient in Ganoderma capenseD.A.Reid cell is released, active drug in Ganoderma capenseD.A.Reid cell enters into fermentation liquid with component portion, after having fermented, fermentation liquid is prepared into thin sesame extractum by evaporation and concentration, and then thin sesame extractum is passed through to baking oven, pulverizer, these tablet manufacturing equipment of tablet machine are prepared into thin sesame sheet, the thin sesame sheet being prepared into is like this to adopt natural material to make completely, safety is good, can treat neurasthenia, treatment climacteric syndrome, because the effective medicinal ingredient in Ganoderma capenseD.A.Reid cell is prepared in thin sesame sheet, people take the medicinal ingredient in Ganoderma capenseD.A.Reid after thin sesame sheet and can directly be absorbed by the body like this, human absorptivity is high, be beneficial to people and treat neurasthenia, these diseases of climacteric syndrome, result of use is good, then the Ganoderma capenseD.A.Reid mycelium after having fermented is by centrifugal, dry, pulverizing obtains Ganoderma capenseD.A.Reid mycelium powder, and then by Ganoderma capenseD.A.Reid mycelium powder by alcohol extraction pot, extractor, go ether pot to extract and obtain Ganoderma capenseD.A.Reid mycelium extracting solution, now, active drug in Ganoderma capenseD.A.Reid mycelium uses component distributing in Ganoderma capenseD.A.Reid mycelium extracting solution, again Ganoderma capenseD.A.Reid mycelium extracting solution is passed through to filling machine, these small-volume injection production lines of ampoule bottle are prepared into BUZHIJUN injection, also Ganoderma capenseD.A.Reid mycelium extracting solution first can be mixed with water for injection by dilute preparing tank, wash embedding interlock unit by plastic soft bottle infusion solutions again and carry out embedding, through sterilizing, dry, lamp inspection, after packaging, obtain BUZHIJUN injection, the BUZHIJUN injection being prepared into like this, BUZHIJUN injection can improve body immunity, be beneficial to absorption of human body, good absorbing effect, the preparation method of Ganoderma capenseD.A.Reid medicine of the present invention, make Ganoderma capenseD.A.Reid be prepared into thin sesame sheet from cultivating to, BUZHIJUN injection, BUZHIJUN injection has been realized integrated effect, increase the speed of production of Ganoderma capenseD.A.Reid medicine, therefore, improve the production efficiency of Ganoderma capenseD.A.Reid medicine, improve economic benefit, and Ganoderma capenseD.A.Reid mycelium and the mycelial fermentation liquid of Ganoderma capenseD.A.Reid can be used completely, can not cause raw-material waste, reduce production cost, raw material adopts this natural material of Ganoderma capenseD.A.Reid mycelium completely, safety is good, result of use is good.
In Secondary media, add these inorganic salts of magnesium sulfate and calcium carbonate can increase the nutrition in Ganoderma capenseD.A.Reid mycelium, and in Secondary media, add Vitamin B6 Tablets can allow Ganoderma capenseD.A.Reid mycelium absorb vitamin B6, allow thin sesame sheet, BUZHIJUN injection, the BUZHIJUN injection that Ganoderma capenseD.A.Reid mycelium is prepared into there is this effective medicinal ingredient of vitamin B6, allow human body can absorb this medicinal ingredient, result of use is good.
The effect that the present invention is useful is: the preparation method of Ganoderma capenseD.A.Reid medicine of the present invention, allow Ganoderma capenseD.A.Reid be prepared into thin sesame sheet from cultivating to, BUZHIJUN injection, BUZHIJUN injection has been realized integrated effect, increase the speed of production of Ganoderma capenseD.A.Reid medicine, therefore, improve the production efficiency of Ganoderma capenseD.A.Reid medicine, improve economic benefit, thin sesame sheet can be treated neurasthenia, climacteric syndrome, BUZHIJUN injection, BUZHIJUN injection can improve body immunity, the medicinal effects of Ganoderma capenseD.A.Reid can be given full play of, the effective rate of utilization of Ganoderma capenseD.A.Reid is high, and Ganoderma capenseD.A.Reid mycelium and the mycelial fermentation liquid of Ganoderma capenseD.A.Reid can be used completely, can not cause raw-material waste, reduce production cost, raw material adopts this natural material of Ganoderma capenseD.A.Reid mycelium completely, safety is good, result of use is good, be beneficial to popularization.
Detailed description of the invention
The invention will be further described below:
Embodiment mono-:
The preparation method of this Ganoderma capenseD.A.Reid medicine, comprises the steps:
The first step, just cultivate, choose 10 grams, agar, 10 grams of sucrose, 10 grams of starch, again the raw material of choosing is encased in seed bottle and forms mixture, then in seed bottle, adding water and supplying mixture capacity is 1 liter, after again mixture being stirred, obtain primary culture medium, then seed bottle is placed to lower exhaust pressure steam sterilizer and carries out sterilization operation, sterilizing time is 30 minutes, after sterilization, treat that seed bottle temperature declines, in the time that seed bottle temperature drops to 24 DEG C, in seed bottle, accessing Ganoderma capenseD.A.Reid strain cultivates, keeping ambient temperature is 25 DEG C, treat that Ganoderma capenseD.A.Reid mycelium germination obtains Ganoderma capenseD.A.Reid mycelia,
Second step, amplification culture, choose 10 grams, wheat bran, 4 grams of Vitamin B6 Tablets, 6 grams, magnesium sulfate, 5 grams of calcium carbonate, Vitamin B6 Tablets is smashed into pulverulence that can be water-soluble to pieces, again the raw material of choosing is encased in and in vial, forms mixture, then in vial, adding water and supplying mixture capacity is 1 liter, after again mixture being stirred, obtain Secondary media, vial is placed in lower exhaust pressure steam sterilizer and carries out sterilization operation again, sterilizing time is 35 minutes, then the amount of trying to please is the seed tank of 10 liters, getting 5 parts of Secondary media is encased in seed tank again, Secondary media accounts for 50% of seed tank capacity, then be linked into the amplification culture of carrying out Ganoderma capenseD.A.Reid in Secondary media by just cultivating the Ganoderma capenseD.A.Reid mycelia obtaining, keeping cultivation temperature is 25 DEG C, treat that Ganoderma capenseD.A.Reid mycelia sends out seed tank full and can obtain Ganoderma capenseD.A.Reid mycelium,
The 3rd step, fermentation by being sent in fermentation tank and fermenting after the Ganoderma capenseD.A.Reid mycelium fragmentation obtaining after amplification culture, then drops into the anti crawl agentfroth oil of 5 grams in fermentation tank, and it is 60% that fermentation tank keeps relative humidity, and fermentation temperature is 30 DEG C, and fermentation time is 4 days;
The 4th step, the preparation of thin sesame sheet, Ganoderma capenseD.A.Reid broth extraction after fermentation is completed is in vessel, then the vessel that Ganoderma capenseD.A.Reid fermentation liquid is housed are sent to the evaporation and concentration of carrying out Ganoderma capenseD.A.Reid fermentation liquid in vapourizing furnace, treat that Ganoderma capenseD.A.Reid fermentation liquid forms Ganoderma capenseD.A.Reid extractum, then the Ganoderma capenseD.A.Reid extractum obtaining is sent in baking oven and is dried, oven temperature is 120 DEG C, after to be dried completing, obtain Ganoderma capenseD.A.Reid solid block, then Ganoderma capenseD.A.Reid solid block is sent to and in pulverizer, is ground into Ganoderma capenseD.A.Reid powder, particle diameter is 80 orders, again Ganoderma capenseD.A.Reid powder is sent to and in tablet machine, is prepared into thin sesame sheet,
The 5th step, the preparation of Ganoderma capenseD.A.Reid mycelium extracting solution, Ganoderma capenseD.A.Reid mycelium after fermentation is completed is put into and in centrifugal dehydrator, is carried out centrifuge dehydration, the centrifuge dehydration time is 15 minutes, then centrifuge dehydration Ganoderma capenseD.A.Reid mycelium is later sent in baking oven and is dried, oven temperature is 110 DEG C, after Ganoderma capenseD.A.Reid mycelium is dried, Ganoderma capenseD.A.Reid mycelium is put into and in pulverizer, is ground into Ganoderma capenseD.A.Reid mycelium powder, the particle diameter of Ganoderma capenseD.A.Reid mycelium powder is 40 orders, again Ganoderma capenseD.A.Reid mycelium powder is put in alcohol extraction pot, then in alcohol extraction pot, add organic solvent, organic solvent and the abundant mix and blend of Ganoderma capenseD.A.Reid mycelium powder are formed to mixture, the ratio of Ganoderma capenseD.A.Reid mycelium powder and organic water solvent is 1:5, then this mixture is put in extractor and extracted, mixture after again extraction being completed is put into and in ether pot, is removed ether or go alcohol operation to obtain Ganoderma capenseD.A.Reid mycelium extracting solution,
The 6th step, the preparation of BUZHIJUN injection, the Ganoderma capenseD.A.Reid mycelium extracting solution and the water for injection that obtain are mixed to get to mixed liquor, the ratio of Ganoderma capenseD.A.Reid mycelium extracting solution and water for injection is 1:500, then by filling machine, this mixed liquor is filled to and in ampoule bottle, is prepared into BUZHIJUN injection;
The 7th step, the preparation of BUZHIJUN injection, the Ganoderma capenseD.A.Reid mycelium extracting solution and the water for injection that obtain are mixed to get to mixed liquor by dilute preparing tank, the ratio of Ganoderma capenseD.A.Reid mycelium extracting solution and water for injection is 1:1000, and then this mixed liquor is washed to embedding interlock unit by plastic soft bottle infusion solutions and carries out embedding and obtain infusion bottle, then by this infusion bottle successively by ultraviolet germicidal lamp sterilization, oven drying, lamp inspection, be packaged to be BUZHIJUN injection.
Further, fermentation tank is mechanical agitation type fermentation tank.
Further, pulverizer is small-sized super micron mill.
Further, tablet machine is Highspeedrotarytabletpress.
Further, organic solvent is the one in ethanol, ether.
Further, filling machine is liquid-filling machine.
Further, anti crawl agentfroth oil is soybean oil.
The preparation method of this Ganoderma capenseD.A.Reid medicine, comprises the steps:
The first step, just cultivate, choose 15 grams, agar, 15 grams of sucrose, 12 grams of starch, again the raw material of choosing is encased in seed bottle and forms mixture, then in seed bottle, adding water and supplying mixture capacity is 1 liter, after again mixture being stirred, obtain primary culture medium, then seed bottle is placed to lower exhaust pressure steam sterilizer and carries out sterilization operation, sterilizing time is 35 minutes, after sterilization, treat that seed bottle temperature declines, in the time that seed bottle temperature drops to 25 DEG C, in seed bottle, accessing Ganoderma capenseD.A.Reid strain cultivates, keeping ambient temperature is 26 DEG C, treat that Ganoderma capenseD.A.Reid mycelium germination obtains Ganoderma capenseD.A.Reid mycelia,
Second step, amplification culture, choose 15 grams, wheat bran, 6 grams of Vitamin B6 Tablets, 8 grams, magnesium sulfate, 8 grams of calcium carbonate, Vitamin B6 Tablets is smashed into pulverulence that can be water-soluble to pieces, again the raw material of choosing is encased in and in vial, forms mixture, then in vial, adding water and supplying mixture capacity is 1 liter, after again mixture being stirred, obtain Secondary media, vial is placed in lower exhaust pressure steam sterilizer and carries out sterilization operation again, sterilizing time is 40 minutes, then the amount of trying to please is the seed tank of 10 liters, getting 5 parts of Secondary media is encased in seed tank again, Secondary media accounts for 50% of seed tank capacity, then be linked into the amplification culture of carrying out Ganoderma capenseD.A.Reid in Secondary media by just cultivating the Ganoderma capenseD.A.Reid mycelia obtaining, keeping cultivation temperature is 26 DEG C, treat that Ganoderma capenseD.A.Reid mycelia sends out seed tank full and can obtain Ganoderma capenseD.A.Reid mycelium,
The 3rd step, fermentation by being sent in fermentation tank and fermenting after the Ganoderma capenseD.A.Reid mycelium fragmentation obtaining after amplification culture, then drops into the anti crawl agentfroth oil of 8 grams in fermentation tank, and it is 70% that fermentation tank keeps relative humidity, and fermentation temperature is 35 DEG C, and fermentation time is 5 days;
The 4th step, the preparation of thin sesame sheet, Ganoderma capenseD.A.Reid broth extraction after fermentation is completed is in vessel, then the vessel that Ganoderma capenseD.A.Reid fermentation liquid is housed are sent to the evaporation and concentration of carrying out Ganoderma capenseD.A.Reid fermentation liquid in vapourizing furnace, treat that Ganoderma capenseD.A.Reid fermentation liquid forms Ganoderma capenseD.A.Reid extractum, then the Ganoderma capenseD.A.Reid extractum obtaining is sent in baking oven and is dried, oven temperature is 130 DEG C, after to be dried completing, obtain Ganoderma capenseD.A.Reid solid block, then Ganoderma capenseD.A.Reid solid block is sent to and in pulverizer, is ground into Ganoderma capenseD.A.Reid powder, particle diameter is 90 orders, again Ganoderma capenseD.A.Reid powder is sent to and in tablet machine, is prepared into thin sesame sheet,
The 5th step, the preparation of Ganoderma capenseD.A.Reid mycelium extracting solution, Ganoderma capenseD.A.Reid mycelium after fermentation is completed is put into and in centrifugal dehydrator, is carried out centrifuge dehydration, the centrifuge dehydration time is 18 minutes, then centrifuge dehydration Ganoderma capenseD.A.Reid mycelium is later sent in baking oven and is dried, oven temperature is 120 DEG C, after Ganoderma capenseD.A.Reid mycelium is dried, Ganoderma capenseD.A.Reid mycelium is put into and in pulverizer, is ground into Ganoderma capenseD.A.Reid mycelium powder, the particle diameter of Ganoderma capenseD.A.Reid mycelium powder is 60 orders, again Ganoderma capenseD.A.Reid mycelium powder is put in alcohol extraction pot, then in alcohol extraction pot, add organic solvent, organic solvent and the abundant mix and blend of Ganoderma capenseD.A.Reid mycelium powder are formed to mixture, the ratio of Ganoderma capenseD.A.Reid mycelium powder and organic water solvent is 1:5, then this mixture is put in extractor and extracted, mixture after again extraction being completed is put into and in ether pot, is removed ether or go alcohol operation to obtain Ganoderma capenseD.A.Reid mycelium extracting solution,
The 6th step, the preparation of BUZHIJUN injection, the Ganoderma capenseD.A.Reid mycelium extracting solution and the water for injection that obtain are mixed to get to mixed liquor, the ratio of Ganoderma capenseD.A.Reid mycelium extracting solution and water for injection is 1:500, then by filling machine, this mixed liquor is filled to and in ampoule bottle, is prepared into BUZHIJUN injection;
The 7th step, the preparation of BUZHIJUN injection, the Ganoderma capenseD.A.Reid mycelium extracting solution and the water for injection that obtain are mixed to get to mixed liquor by dilute preparing tank, the ratio of Ganoderma capenseD.A.Reid mycelium extracting solution and water for injection is 1:1000, and then this mixed liquor is washed to embedding interlock unit by plastic soft bottle infusion solutions and carries out embedding and obtain infusion bottle, then by this infusion bottle successively by ultraviolet germicidal lamp sterilization, oven drying, lamp inspection, be packaged to be BUZHIJUN injection.
Further, fermentation tank is mechanical agitation type fermentation tank.
Further, pulverizer is small-sized super micron mill.
Further, tablet machine is Highspeedrotarytabletpress.
Further, organic solvent is the one in ethanol, ether.
Further, filling machine is liquid-filling machine.
Further, anti crawl agentfroth oil is soybean oil.
The preparation method of this Ganoderma capenseD.A.Reid medicine, comprises the steps:
The first step, just cultivate, choose 20 grams, agar, 20 grams of sucrose, 15 grams of starch, again the raw material of choosing is encased in seed bottle and forms mixture, then in seed bottle, adding water and supplying mixture capacity is 1 liter, after again mixture being stirred, obtain primary culture medium, then seed bottle is placed to lower exhaust pressure steam sterilizer and carries out sterilization operation, sterilizing time is 40 minutes, after sterilization, treat that seed bottle temperature declines, in the time that seed bottle temperature drops to 26 DEG C, in seed bottle, accessing Ganoderma capenseD.A.Reid strain cultivates, keeping ambient temperature is 27 DEG C, treat that Ganoderma capenseD.A.Reid mycelium germination obtains Ganoderma capenseD.A.Reid mycelia,
Second step, amplification culture, choose 20 grams, wheat bran, 8 grams of Vitamin B6 Tablets, 12 grams, magnesium sulfate, 10 grams of calcium carbonate, Vitamin B6 Tablets is smashed into pulverulence that can be water-soluble to pieces, again the raw material of choosing is encased in and in vial, forms mixture, then in vial, adding water and supplying mixture capacity is 1 liter, after again mixture being stirred, obtain Secondary media, vial is placed in lower exhaust pressure steam sterilizer and carries out sterilization operation again, sterilizing time is 45 minutes, then the amount of trying to please is the seed tank of 10 liters, getting 5 parts of Secondary media is encased in seed tank again, Secondary media accounts for 50% of seed tank capacity, then be linked into the amplification culture of carrying out Ganoderma capenseD.A.Reid in Secondary media by just cultivating the Ganoderma capenseD.A.Reid mycelia obtaining, keeping cultivation temperature is 27 DEG C, treat that Ganoderma capenseD.A.Reid mycelia sends out seed tank full and can obtain Ganoderma capenseD.A.Reid mycelium,
The 3rd step, fermentation by being sent in fermentation tank and fermenting after the Ganoderma capenseD.A.Reid mycelium fragmentation obtaining after amplification culture, then drops into the anti crawl agentfroth oil of 10 grams in fermentation tank, and it is 80% that fermentation tank keeps relative humidity, and fermentation temperature is 40 DEG C, and fermentation time is 6 days;
The 4th step, the preparation of thin sesame sheet, Ganoderma capenseD.A.Reid broth extraction after fermentation is completed is in vessel, then the vessel that Ganoderma capenseD.A.Reid fermentation liquid is housed are sent to the evaporation and concentration of carrying out Ganoderma capenseD.A.Reid fermentation liquid in vapourizing furnace, treat that Ganoderma capenseD.A.Reid fermentation liquid forms Ganoderma capenseD.A.Reid extractum, then the Ganoderma capenseD.A.Reid extractum obtaining is sent in baking oven and is dried, oven temperature is 140 DEG C, after to be dried completing, obtain Ganoderma capenseD.A.Reid solid block, then Ganoderma capenseD.A.Reid solid block is sent to and in pulverizer, is ground into Ganoderma capenseD.A.Reid powder, particle diameter is 100 orders, again Ganoderma capenseD.A.Reid powder is sent to and in tablet machine, is prepared into thin sesame sheet,
The 5th step, the preparation of Ganoderma capenseD.A.Reid mycelium extracting solution, Ganoderma capenseD.A.Reid mycelium after fermentation is completed is put into and in centrifugal dehydrator, is carried out centrifuge dehydration, the centrifuge dehydration time is 20 minutes, then centrifuge dehydration Ganoderma capenseD.A.Reid mycelium is later sent in baking oven and is dried, oven temperature is 130 DEG C, after Ganoderma capenseD.A.Reid mycelium is dried, Ganoderma capenseD.A.Reid mycelium is put into and in pulverizer, is ground into Ganoderma capenseD.A.Reid mycelium powder, the particle diameter of Ganoderma capenseD.A.Reid mycelium powder is 70 orders, again Ganoderma capenseD.A.Reid mycelium powder is put in alcohol extraction pot, then in alcohol extraction pot, add organic solvent, organic solvent and the abundant mix and blend of Ganoderma capenseD.A.Reid mycelium powder are formed to mixture, the ratio of Ganoderma capenseD.A.Reid mycelium powder and organic water solvent is 1:5, then this mixture is put in extractor and extracted, mixture after again extraction being completed is put into and in ether pot, is removed ether or go alcohol operation to obtain Ganoderma capenseD.A.Reid mycelium extracting solution,
The 6th step, the preparation of BUZHIJUN injection, the Ganoderma capenseD.A.Reid mycelium extracting solution and the water for injection that obtain are mixed to get to mixed liquor, the ratio of Ganoderma capenseD.A.Reid mycelium extracting solution and water for injection is 1:500, then by filling machine, this mixed liquor is filled to and in ampoule bottle, is prepared into BUZHIJUN injection;
The 7th step, the preparation of BUZHIJUN injection, the Ganoderma capenseD.A.Reid mycelium extracting solution and the water for injection that obtain are mixed to get to mixed liquor by dilute preparing tank, the ratio of Ganoderma capenseD.A.Reid mycelium extracting solution and water for injection is 1:1000, and then this mixed liquor is washed to embedding interlock unit by plastic soft bottle infusion solutions and carries out embedding and obtain infusion bottle, then by this infusion bottle successively by ultraviolet germicidal lamp sterilization, oven drying, lamp inspection, be packaged to be BUZHIJUN injection.
Further, fermentation tank is mechanical agitation type fermentation tank.
Further, pulverizer is small-sized super micron mill.
Further, tablet machine is Highspeedrotarytabletpress.
Further, organic solvent is the one in ethanol, ether.
Further, filling machine is liquid-filling machine.
Further, anti crawl agentfroth oil is soybean oil.
The feature of the embodiment of the present invention is: the preparation method of Ganoderma capenseD.A.Reid medicine of the present invention, allow Ganoderma capenseD.A.Reid be prepared into thin sesame sheet from cultivating to, BUZHIJUN injection, BUZHIJUN injection has been realized integrated effect, increase the speed of production of Ganoderma capenseD.A.Reid medicine, therefore, improve the production efficiency of Ganoderma capenseD.A.Reid medicine, improve economic benefit, thin sesame sheet can be treated neurasthenia, climacteric syndrome, BUZHIJUN injection, BUZHIJUN injection can improve body immunity, the medicinal effects of Ganoderma capenseD.A.Reid can be given full play of, the effective rate of utilization of Ganoderma capenseD.A.Reid is high, and Ganoderma capenseD.A.Reid mycelium and the mycelial fermentation liquid of Ganoderma capenseD.A.Reid can be used completely, can not cause raw-material waste, reduce production cost, raw material adopts this natural material of Ganoderma capenseD.A.Reid mycelium completely, safety is good, result of use is good, be beneficial to popularization.
Ganoderma capenseD.A.Reid medicine prepared by the preparation method of Ganoderma capenseD.A.Reid medicine of the present invention, adopts the mode of second incubation to cultivate Ganoderma capenseD.A.Reid mycelium, can increase the mycelial speed of growth of Ganoderma capenseD.A.Reid, has improved efficiency, and result of use is good.
Get BUZHIJUN injection and Chinese medicine standard prepared by BUZHIJUN injection prepared by BUZHIJUN injection prepared by 100 embodiment of the present invention one, 100 parts of embodiment of the present invention two, 100 parts of embodiment of the present invention three and contrast, comparing result is as follows:
| project | statutory standards | embodiment mono-end product quality standard | embodiment bis-end product quality standards | embodiment tri-end product quality standards |
| character | should be brown color to henna clear liquid | for brown color is to henna clear liquid | for brown color is to henna clear liquid | for brown color is to henna clear liquid |
| pH value | 5.0～7.0 | 5.5～7.0 | 5.4～7.0 | 5.6～7.0 |
| protein | / | conform with the regulations | conform with the regulations | conform with the regulations |
| undue toxicity | should conform with the regulations | conform with the regulations | conform with the regulations | conform with the regulations |
| pyrogen | / | by rabbit body weight 1kg injection 1ml, conform with the regulations | by rabbit body weight 1kg injection 1ml, conform with the regulations | by rabbit body weight 1kg injection 1ml, conform with the regulations |
| haemolysis and cohesion | / | conform with the regulations | conform with the regulations | conform with the regulations |
| tannin | / | conform with the regulations | conform with the regulations | conform with the regulations |
| total solid | / | conform with the regulations | conform with the regulations | conform with the regulations |
| loading amount | 5 all must not be less than 2ml | 5 are all no less than 2ml | 5 are all no less than 2ml | 5 are all no less than 2ml |
| visible foreign matters | 20 all must not check out visible foreign matters, as detect fine visible foreign matters, should separately get 20, and with method retrial, just, in the experimental cultivar of retrial, the test sample that detects fine visible must not exceed two | 20 are without visible foreign matters, without fine foreign body. | 20 are without visible foreign matters, without fine foreign body. | 20 are without visible foreign matters, and 1 checks out fine foreign body, separately get 20 employings with method retrial, without fine foreign body |
| aseptic | should conform with the regulations | conform with the regulations | conform with the regulations | conform with the regulations |
| finger printing | / | similarity is not less than 0.90 | similarity is not less than 0.90 | similarity is not less than 0.90 |
| nitrogen content | / | every nitrogen content is no less than 3.0mg | every nitrogen content is no less than 3.0mg | every nitrogen content is no less than 3.0mg |
| adenosine content | every contains Bozhi Mycelium powder in adenosine (C10H13N5O4), must not be less than 0.12mg | every contains Bozhi Mycelium powder in adenosine (C10H13N5O4), is no less than 0.12mg | every contains Bozhi Mycelium powder in adenosine (C10H13N5O4), is no less than 0.12mg | every contains Bozhi Mycelium powder in adenosine (C10H13N5O4), is no less than 0.12mg |
As can be known from the above table, Ganoderma capenseD.A.Reid medicine prepared by the preparation method of Ganoderma capenseD.A.Reid medicine of the present invention, has met the statutory standards of Chinese medicine completely, can use as Chinese medicine completely.
Although the present invention is described by reference to preferred embodiment,, those skilled in the art should understand, and in the scope of claims, can do the various variation in form and details.
Claims (7)
1. a preparation method for Ganoderma capenseD.A.Reid medicine, is characterized in that comprising the steps:
(1): just cultivate, choose 10 parts～20 parts, agar, 10 parts～20 parts of sucrose, 10 parts～15 parts of starch, again the raw material of choosing is encased in seed bottle and forms mixture, then in seed bottle, adding water and supplying mixture capacity is 1 liter, after again mixture being stirred, obtain primary culture medium, then seed bottle is placed to lower exhaust pressure steam sterilizer and carries out sterilization operation, sterilizing time is 30 minutes～40 minutes, after sterilization, treat that seed bottle temperature declines, in the time that seed bottle temperature drops to 24 DEG C～26 DEG C, in seed bottle, accessing Ganoderma capenseD.A.Reid strain cultivates, keeping ambient temperature is 25 DEG C～27 DEG C, treat that Ganoderma capenseD.A.Reid mycelium germination obtains Ganoderma capenseD.A.Reid mycelia,
(2): amplification culture, choose 10 parts～20 parts, wheat bran, 4 parts～8 parts of Vitamin B6 Tablets, 6 parts～12 parts, magnesium sulfate, 5 parts～10 parts of calcium carbonate, Vitamin B6 Tablets is smashed into pulverulence that can be water-soluble to pieces, again the raw material of choosing is encased in and in vial, forms mixture, then in vial, adding water and supplying mixture capacity is 1 liter, after again mixture being stirred, obtain Secondary media, vial is placed in lower exhaust pressure steam sterilizer and carries out sterilization operation again, sterilizing time is 35 minutes～45 minutes, then the amount of trying to please is the seed tank of 10 liters, getting 5 parts of Secondary media is encased in seed tank again, Secondary media accounts for 50% of seed tank capacity, then be linked into the amplification culture of carrying out Ganoderma capenseD.A.Reid in Secondary media by just cultivating the Ganoderma capenseD.A.Reid mycelia obtaining, keeping cultivation temperature is 25 DEG C～27 DEG C, treat that Ganoderma capenseD.A.Reid mycelia sends out seed tank full and can obtain Ganoderma capenseD.A.Reid mycelium,
(3): fermentation, by being sent in fermentation tank and fermenting after the Ganoderma capenseD.A.Reid mycelium fragmentation obtaining after amplification culture, then in fermentation tank, drop into the anti crawl agentfroth oil of 5 parts～10 parts, it is 60%～80% that fermentation tank keeps relative humidity, fermentation temperature is 30 DEG C～40 DEG C, and fermentation time is 4 days～6 days;
(4): the preparation of thin sesame sheet, Ganoderma capenseD.A.Reid broth extraction after fermentation is completed is in vessel, then the vessel that Ganoderma capenseD.A.Reid fermentation liquid is housed are sent to the evaporation and concentration of carrying out Ganoderma capenseD.A.Reid fermentation liquid in vapourizing furnace, treat that Ganoderma capenseD.A.Reid fermentation liquid forms Ganoderma capenseD.A.Reid extractum, then the Ganoderma capenseD.A.Reid extractum obtaining is sent in baking oven and is dried, oven temperature is 120 DEG C～140 DEG C, after to be dried completing, obtain Ganoderma capenseD.A.Reid solid block, then Ganoderma capenseD.A.Reid solid block is sent to and in pulverizer, is ground into Ganoderma capenseD.A.Reid powder, particle diameter is 80 order～100 orders, again Ganoderma capenseD.A.Reid powder is sent to and in tablet machine, is prepared into thin sesame sheet,
(5): the preparation of Ganoderma capenseD.A.Reid mycelium extracting solution, Ganoderma capenseD.A.Reid mycelium after fermentation is completed is put into and in centrifugal dehydrator, is carried out centrifuge dehydration, the centrifuge dehydration time is 15 minutes～20 minutes, then centrifuge dehydration Ganoderma capenseD.A.Reid mycelium is later sent in baking oven and is dried, oven temperature is 110 DEG C～130 DEG C, after Ganoderma capenseD.A.Reid mycelium is dried, Ganoderma capenseD.A.Reid mycelium is put into and in pulverizer, is ground into Ganoderma capenseD.A.Reid mycelium powder, the particle diameter of Ganoderma capenseD.A.Reid mycelium powder is 40 order～70 orders, again Ganoderma capenseD.A.Reid mycelium powder is put in alcohol extraction pot, then in alcohol extraction pot, add organic solvent, organic solvent and the abundant mix and blend of Ganoderma capenseD.A.Reid mycelium powder are formed to mixture, the ratio of Ganoderma capenseD.A.Reid mycelium powder and organic water solvent is 1:5, then this mixture is put in extractor and extracted, mixture after again extraction being completed is put into and in ether pot, is removed ether or go alcohol operation to obtain Ganoderma capenseD.A.Reid mycelium extracting solution,
(5): the preparation of BUZHIJUN injection, the Ganoderma capenseD.A.Reid mycelium extracting solution and the water for injection that obtain are mixed to get to mixed liquor, the ratio of Ganoderma capenseD.A.Reid mycelium extracting solution and water for injection is 1:500, then by filling machine, this mixed liquor is filled to and in ampoule bottle, is prepared into BUZHIJUN injection;
(7): the preparation of BUZHIJUN injection, the Ganoderma capenseD.A.Reid mycelium extracting solution and the water for injection that obtain are mixed to get to mixed liquor by dilute preparing tank, the ratio of Ganoderma capenseD.A.Reid mycelium extracting solution and water for injection is 1:1000, and then this mixed liquor is washed to embedding interlock unit by plastic soft bottle infusion solutions and carries out embedding and obtain infusion bottle, then by this infusion bottle successively by ultraviolet germicidal lamp sterilization, oven drying, lamp inspection, be packaged to be BUZHIJUN injection.
2. the preparation method of Ganoderma capenseD.A.Reid medicine according to claim 1, is characterized in that: the fermentation tank in described step (2) is mechanical agitation type fermentation tank.
3. the preparation method of Ganoderma capenseD.A.Reid medicine according to claim 1, is characterized in that: the pulverizer in described step (3) and step (4) is small-sized super micron mill.
4. the preparation method of Ganoderma capenseD.A.Reid medicine according to claim 1, is characterized in that: the tablet machine in described step (3) is Highspeedrotarytabletpress.
5. the preparation method of Ganoderma capenseD.A.Reid medicine according to claim 1, is characterized in that: the organic solvent in described step (4) is the one in ethanol, ether.
6. the preparation method of Ganoderma capenseD.A.Reid medicine according to claim 1, is characterized in that: the filling machine in described step (5) is liquid-filling machine.
7. the preparation method of Ganoderma capenseD.A.Reid medicine according to claim 1, is characterized in that: the anti crawl agentfroth oil in described step (3) is soybean oil.
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