CN104585244A - Method for preparing radix fici simplicissimae extract, radix fici simplicissimae extract and application of extract - Google Patents
Method for preparing radix fici simplicissimae extract, radix fici simplicissimae extract and application of extract Download PDFInfo
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- CN104585244A CN104585244A CN201510002631.1A CN201510002631A CN104585244A CN 104585244 A CN104585244 A CN 104585244A CN 201510002631 A CN201510002631 A CN 201510002631A CN 104585244 A CN104585244 A CN 104585244A
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- radix fici
- oranges
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- fici simplicissimae
- tangerines
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- 239000008194 pharmaceutical composition Substances 0.000 claims abstract description 7
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- 241000675108 Citrus tangerina Species 0.000 claims description 44
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 30
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Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides a method for preparing a radix fici simplicissimae extract. The method comprises the step of performing ultrasonic-assisted ethanol extraction on radix fici simplicissimae. The invention also provides the radix fici simplicissimae extract obtained by the preparation method, and an application of the radix fici simplicissimae extract in preparation of pharmaceutical compositions for inhibiting pathogenic bacteria after citrus harvesting. The radix fici simplicissimae extract has a good effect of inhibiting the pathogenic bacteria after citrus harvesting, is used for eliminating chemical residues after citrus preservation, does not have health hazards to the human body and is environment-friendly, and the resistance to drugs is difficultly caused.
Description
Technical field
The invention belongs to fruit disease control and fresh-keeping field, be specifically related to a kind of to there is radix fici simplicissimatis extractive of suppression oranges and tangerines postharvest pathogen effect and preparation method thereof and novelty teabag.
Background technology
Oranges and tangerines are the large trade fruit of the first in the world, are also the third-largest trade agricultural product.China's oranges and tangerines aboundresources, plant with a long history.Cultivated area and the output of current China oranges and tangerines all rank first in the world, and within 2013, oranges and tangerines cultivated area reaches more than 3,600 ten thousand mu, and gross yield is about more than 3,200 ten thousand tons, and Citrus Industry occupies very important status in China's agricultural economy.
Citrusfruit is organized delicate succulence and is rich in sugar, in the processes such as harvesting, storing and sale, mechanical damage easily occurs, and is subject to multiple infecting of disease fungus and produces postharvest disease after pericarp damage.The pathologic disease species that rear generation adopted by oranges and tangerines reaches more than 20 kinds, mainly contain penicilliosis that Italian mould (Penicillium italicum) causes and the green mould that Penicillium digitatum (Penicillium digitatum) causes, these diseases easily cause fruit rot to go bad, rotting rate is generally 10% ~ 30%, up to more than 40% ~ 50%, huge economic loss can be caused time serious.Other pathologic disease of oranges and tangerines also comprises black rot, sour rot, brown rot, brown Supreme Being maize ear rot, anthracnose and burnt maize ear rot etc.
For a long time, people mainly adopt chemosynthesis material to carry out fresh-keeping as antistaling agent to oranges and tangerines, and the chemical bactericide that different times uses is different.Borax, sorbic acid and its esters etc. are the oranges and tangerines preservatives used the earliest; The benzimidazole germicide comprising benomyl (Benomyl), topsin (Thiophanate), thiophanate methyl (Thiophanatemethyl), carbendazim (Carbendazim), probenazole (Thiabendazole) etc. is the second generation oranges and tangerines preservative of the application seventies; The third generation oranges and tangerines preservative of current use comprises imazalil (Imazalil), guazatine (Guazatine), Prochloraz (Prochloraz) etc.These chemical bactericides can kill or suppress the growth of pathogenic microorganism, have good corrosion-resistanting fresh-keeping effect.Because chemical bactericide can cause, medicament is residual, contaminated environment, and Long-Time Service causes oranges and tangerines to adopt the reasons such as rear germ develops immunity to drugs making the safety in utilization of chemical bactericide day by day obtain the concern of people.In addition, along with science and technology development, detect improving and the further investigation of chemical bactericide toxicity of analysis means, find that some chemical preservatives have the possibility of carcinogenic or potential carcinogenic, mutagenesis, teratogenesis.Some chemical bactericides have been forbidden by developed countries such as America and Europes or have limited use at present, and as benomyl and guazatine series bactericidal agent etc., oneself is prohibited use or restriction use in part fruit products by EPA.
In recent years, people start the research and development turning to natural plant antistaling agent.The advantages such as natural plant antistaling agent has efficiently, low toxicity, low-residual, pollution-free, use safety, adopt its process oranges and tangerines to carry out anti-corrosive fresh-keeping and will be subject to the popular welcome of consumer.In addition, medicine based on herbs is by multicomponent, and Mutiple Targets plays bacteriostasis, not easily develops immunity to drugs.Therefore, be necessary to find from natural plants, exploitation natural plant source antiseptic agent carries out oranges and tangerines fresh-keeping to reduce dependence to chemical agent.
The root that radix fici simplicissimae (Ficus simplicissima Lour.) is Moraceae fig cleft palm banyan, records in the plurality of Chinese books such as " Chinese Pharmacopoeia ", " Chinese medicine voluminous dictionary " over the years, " national Chinese herbal medicine compilation ", " Chinese medicinal herbal ", " China's book on Chinese herbal medicine ".Radix fici simplicissimae is the genuine traditional Chinese medicine material of Guangdong Lingnan area, has another name called cairo morningglory root or leaf, sub-fork wood, five pawl wild peach, five pawl milk, Herba Fici Simplicissimae, southern stilbene etc., has the effect that well replenishes qi to invigorate the spleen, have the good reputation of " panacis quinquefolii radix ".Radix fici simplicissimae property is put down, and taste is sweet, pungent, have strengthening the spleen and tonifying the lung, the merit of dampness removing relaxing muscles and tendons, can be used for insufficiency of the spleen edema, food few unable, pulmonary tuberculosis cough, night sweat, arthralgia due to wind-dampness, postpartum the disease such as agalasisa, in Guangdong, use among the people is with a long history.Modern pharmacology activity research shows, radix fici simplicissimae can play protective effect by improving microcirculation to stomach lining, alleviates liver tissue injury, improves body's immunity, and (Wang Wei is big, Chen Yao to alleviate the infringement of cerebral hypoxia ischemia; The chemical composition of radix fici simplicissimae and Advance on Pharmacological Activities; " China National folk medicine ", 2013,22 (3): 41-42).About the safety that radix fici simplicissimae uses,, after mouse gavaging radix fici simplicissimae water extract in 14d, animal is all without death, and eye, nose, mouth are without mucus secretion to have animal acute toxicity experiment research to show, body weight, amount of drinking water and food-intake and blank group are without significant difference, and each internal organs are all without exception.Show that radix fici simplicissimae toxicity is extremely low, biological safety is good, and (Luo Qian, Xi Ping, Liao Xuezhen, Cai Qingqun recruit Rong Jian; The safety pharmacology experimental study of radix fici simplicissimae decocting liquid; " pharmacy today ", 2009,19 (2): 12-13).
Many sections of patent documents also disclose the medical usage of radix fici simplicissimae.As, the Chinese invention patent application of denomination of invention " radix fici simplicissimae is preparing the application in antineoplastic " (publication number CN103156922A), the extract disclosing radix fici simplicissimae has the effect of In Vitro Anti Breast cancer lines Bcap-37, and in vivo and Induction in vitro TNF (TNF), antineoplastic action can be had.The Chinese invention patent application of denomination of invention " a kind of radix fici simplicissimatis extractive and its production and use " (publication number CN101129473A), discloses radix fici simplicissimatis extractive and is preparing the application in the medicines such as acute/chronic bronchitis, asthma, pulmonary emphysema, chronic obstructive pulmonary disease, hypoimmunity disease medicament, virus hepatitis, chronic hepatitis B.The Chinese invention patent application of denomination of invention " purposes of radix fici simplicissimae in the preparation adjuvant therapy medicaments of hepatitis and health food " (publication number CN1660354A), disclosing with radix fici simplicissimatis extractive is that the medicine of active component or health food can treat paracetamol toxic hepatitis, carbon tetrachloride poisoning hepatitis and cocaine poisoning hepatitis effectively.
By literature search, the antibacterial activity about radix fici simplicissimae is studied, and rarely seen radix fici simplicissimae water boiling and extraction liquid has inhibitory action (Chen Qiong, Ye Sixia to Escherichia coli, Bacillus subtillis, staphylococcus aureus; Trace determination of color radix fici simplicissimae bacteriostatic activity, " Agriculture of Anhui science ", 2012, (15): 8452-8454), but inhibitory action (Wang Xiaoping, Duan Liju, Chen Xiaobai are not had to aspergillus niger, aspergillus flavus, Li Limei, Lu Qifeng, Li Jianqing; The experimental study of radix fici simplicissimae Aqueous extracts vitro antibacterial activity, " hour hands traditional Chinese medical science traditional Chinese medicines ", 2010,21 (7): 1692-1693).
In addition, the Chinese invention patent application of publication number CN101138585A discloses a kind of method of supercritical carbon dioxide extracting radix fici simplicissimae active ingredient.It is expensive to there is instrument and equipment in the method, is only applicable to the extraction of lipophilicity, molecular weight material.
Visible prior art to the research primary limitation of radix fici simplicissimae with the medical usage of artificial subject.So far, there is not yet the report of radix fici simplicissimatis extractive to the effect of oranges and tangerines postharvest pathogenic fungi, also do not extract about ultrasonic assistant the report preparing radix fici simplicissimatis extractive.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, a kind of preparation method of radix fici simplicissimatis extractive and the radix fici simplicissimatis extractive novelty teabag of preparation thereof are provided.The present invention is with natural Chinese herbal medicine for raw material, and the radix fici simplicissimatis extractive of preparation to the fungistatic effect of oranges and tangerines postharvest pathogenic fungi obviously and without chemical residual, to human body without health hazard, environmentally safe, imply that good application prospect.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
A preparation method for radix fici simplicissimatis extractive, adopts ultrasonic assistant to extract, take ethanol as Extraction solvent, comprise the steps:
The mass percent concentration adding radix fici simplicissimae weight 10 ~ 40 times in described radix fici simplicissimae is the ethanol of 60% ~ 100%, is 40 ~ 60 DEG C at Extracting temperature, and extraction time is 30min ~ 150min, and supersonic frequency is extract under the condition of 30 ~ 40KHz; Filter, reclaim ethanol, concentrated, to obtain final product.
In preparation method of the present invention, preferably, the weight of ethanol is 20 ~ 40 times of described radix fici simplicissimae weight; Be more preferably 30 times.
Preferably, the mass percent concentration of ethanol is preferably 90% ~ 100%; Be more preferably 90%.
Preferably, ultrasonic assistant extraction time is preferably 30 ~ 90min, is more preferably 60min.
Preferably, the temperature that ultrasonic assistant extracts is 60 DEG C.
Also preferred, before alcohol extract, described radix fici simplicissimae 40 ~ 50 DEG C oven dry, crosses 40 mesh sieves after pulverizing, gets minus sieve powder and carry out ultrasonic assistant extraction.
As a preferred embodiment, the invention provides a kind of preparation method of radix fici simplicissimatis extractive, comprise the steps:
I. by radix fici simplicissimae 40 ~ 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the mass percent concentration adding described radix fici simplicissimae weight 20 ~ 40 times in the powder that step I obtains be 90% ~ 100% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 40 DEG C ~ 60 DEG C, extraction time is 60min ~ 90min, and supersonic frequency is 30 ~ 40KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, to obtain final product.
As a preferred embodiment, the invention provides a kind of preparation method of radix fici simplicissimatis extractive, comprise the steps:
I. by radix fici simplicissimae 40 ~ 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the mass percent concentration adding described radix fici simplicissimae weight 30 times in the powder that step I obtains be 90% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 60 DEG C, and extraction time is 60min, and supersonic frequency is 30 ~ 40KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, to obtain final product.
Another object of the present invention, is the radix fici simplicissimatis extractive providing above-mentioned preparation method to prepare.
A further object of the invention, is to provide radix fici simplicissimae or radix fici simplicissimatis extractive for the preparation of the purposes in the pharmaceutical composition of control oranges and tangerines postharvest pathogen and/or the agent of oranges and tangerines post-harvest fresh-keeping; Preferably, described radix fici simplicissimatis extractive is prepared by preparation method of the present invention.
Described oranges and tangerines postharvest pathogen is selected from Italian mould (Penicillium italicum), Penicillium digitatum (Penicillium digitatum), one or more in citric acid maize ear rot bacterium (Geotrichum candidum) and oranges and tangerines black rot (Alternariacitri).
Thus, the invention provides a kind of pharmaceutical composition preventing and treating oranges and tangerines postharvest pathogen, comprise the radix fici simplicissimatis extractive that preparation method of the present invention prepares.
In addition, the present invention also provides the agent of a kind of oranges and tangerines post-harvest fresh-keeping, comprises the described radix fici simplicissimatis extractive that preparation method of the present invention prepares.
Pharmaceutical composition of the present invention or the agent of oranges and tangerines post-harvest fresh-keeping, except described radix fici simplicissimatis extractive, acceptable auxiliary material can also be comprised, such as be selected from the film forming agent such as carboxymethyl cellulose, shitosan, be selected from the cosolvent of glycerine, sucrose ester, tween etc., be selected from the preservative of citric acid, calcium propionate, potassium sorbate or Sodium Benzoate etc., and suitable solvent, as water.
Ultrasonic has " cavitation effect ", the characteristic such as " mechanical oscillation " and " fuel factor ", " cavitation effect " can produce several thousand atmospheric pressures instantaneously, the micro-bubble of Extraction medium is compressed, bursts, the broken cell wall extracting raw material, accelerate the stripping of natural medicinal component, " mechanical oscillation " and " fuel factor " further enhance the diffusion of stripping composition, make medicinal component reach concentration balance fast in Extraction medium, greatly can shorten extraction cost.And Extracting temperature is lower, avoids the destruction of long-time high-temperature water decoction to radix fici simplicissimae chemical composition, improve the quality of products.
The extract that the present invention obtains for raw material with natural medicine food dual purpose plant radix fici simplicissimae, has very strong suppression Italy mould, Penicillium digitatum, the effect of oranges and tangerines black rot and sour germ.Pharmaceutical composition and the citrus preservative containing radix fici simplicissimatis extractive of the present invention, without chemical residual, to human body without health hazard, environmentally safe, has good application prospect.
Accompanying drawing explanation
The photo display of Fig. 1 be in test example 1, radix fici simplicissimatis extractive prepared by embodiment 1 to the fungistatic effect of Italian mould, Penicillium digitatum, citric acid maize ear rot bacterium and oranges and tangerines black rot, arrow instruction be the edge of bacterial restrain; Wherein 1A display is the fungistatic effect of radix fici simplicissimatis extractive to Italian mould, what 1B showed is the fungistatic effect of radix fici simplicissimatis extractive to Penicillium digitatum, what 1C showed is the fungistatic effect of radix fici simplicissimatis extractive to sour germ, and what 1D showed is the fungistatic effect of radix fici simplicissimatis extractive to black rot.
In the test example 1 of the photo display of Fig. 2, the radix fici simplicissimatis extractive of variable concentrations prepared by embodiment 1 is to the control efficiency of oranges and tangerines being vaccinated with oranges and tangerines black rot.
Embodiment
By concrete example given below, the invention will be further described, but it can not be interpreted as limitation of the invention.
Medicinal material, reagent, biomaterial that following embodiment is used, if no special instructions, all derive from commercial sources.
Embodiment 1 one kinds of radix fici simplicissimatis extractives
Described radix fici simplicissimatis extractive is prepared as follows:
I. by radix fici simplicissimae 40 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the weight percent concentration adding described radix fici simplicissimae weight 20 times in the powder that step I obtains be 95% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 60 DEG C, and extraction time is 2 hours, and supersonic frequency is 30KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, obtains described radix fici simplicissimatis extractive.
Test example 1 radix fici simplicissimatis extractive is to the bacteriostasis of oranges and tangerines postharvest pathogenic fungi
Experiment material:
1. supply reagent thing: radix fici simplicissimatis extractive prepared by embodiment 1, be mixed with distilled water the solution that concentration is 1g medicinal material/mL.
2. strains tested: Italian mould (Penicillium italicum, ACCC30399), Penicillium digitatum (Penicillium digitatum, ACCC30389), all from Chinese agriculture Culture Collection (ACCC); Citric acid maize ear rot bacterium (Geotrichum candidum) and oranges and tangerines black rot (Alternaria citri) laboratory are separated from south, the Jiangxi sick fruit of navel orange and obtain.
Experimental technique:
1, agar diffusion method measures the bacteriostasis of radix fici simplicissimatis extractive to Italian mould and Penicillium digitatum
Get and be inoculated in test tube slant medium for examination bacterium, cultivate 2-3d for 30 DEG C, will wash in triangular flask for examination bacterium PDB culture fluid by spore in test-tube culture medium, shake up, bead be broken up, and makes spore suspension, counts, make bacterial concentration reach 10 with blood counting chamber
6-10
7cfu/mL is for subsequent use.
Draw above-mentioned pityrosporion ovale suspension 5mL to be mixed into and to be cooled to about 50 DEG C PDA medium, preparation band bacterium culture medium.Then place aseptic Oxford cup, respectively at adding radix fici simplicissimatis extractive solution described in 200 μ L in the cup of Oxford, being placed in 28 DEG C of insulating boxs and cultivating 48h, right-angled intersection method measures the size of inhibition zone.Replication three times, calculates inhibition zone diameter mean value.
2, growth rate method measures the bacteriostasis of radix fici simplicissimatis extractive to citric acid maize ear rot bacterium and oranges and tangerines black rot
Under aseptic condition, with pure PDA medium for contrast, the each colony edge bacterium block for examination bacterium got and activated is beaten with diameter 6mm card punch, be transferred to containing in the PDA medium of radix fici simplicissimatis extractive and control medium respectively, each process in triplicate, and is placed in 28 DEG C of constant incubators and cultivates, and records each colony growth situation after 5d, and measure colony diameter by right-angled intersection method, calculate inhibiting rate.
Inhibiting rate (%)=100 × (contrast colony diameter-process colony diameter)/contrast colony diameter
3, doubling dilution measures minimum inhibitory concentration (MIC) and the minimal bactericidal concentration (MFC) of radix fici simplicissimatis extractive
By ten concentration gradients between described radix fici simplicissimatis extractive solution doubling dilution to 1.95mg radix fici simplicissimae medicinal material/mL ~ 1000mg radix fici simplicissimae medicinal material/mL, then the radix fici simplicissimatis extractive solution of 10mL variable concentrations is drawn in the PDA medium do not melted containing 90mL, pour into after shaking up in flat board, make pastille medium, after culture medium solidifying, evenly be applied on pastille PDA medium by Italian mould pityrosporion ovale suspension with spread plate, each concentration in triplicate.And blank group (medium does not inoculate bacterium), bacterium liquid control group (culture medium inoculated bacterium but not containing radix fici simplicissimae ethanol extract) are set.The incubator that experimental group and control group are placed in 28 DEG C is cultivated 2d, in experimental group with the culture dish of asepsis growth minimum containing concentration be its MIC, being continued to cultivate after 7d by the culture dish of all asepsis growths and observe, is its MFC with the culture dish of asepsis growth minimum containing concentration.
4, radix fici simplicissimae ethanol extract is to the biocontrol effect of south, Jiangxi navel orange
By south, Jiangxi Navel Orange Fruits with after the process of 2%NaClO solution disinfection, cleaner with running water, dry for subsequent use.Stab a diameter 4mm with transfer needle at fruit waist, the wound of degree of depth 3mm, inoculate radix fici simplicissimatis extractive and the 20 μ L 1 × 10 of 20 μ L variable concentrations with micropipettor
6cFU/mL Italy mould spore suspension, test point 3 process: 1. citrusfruit inoculates Italian mould spore suspension, as test blank; 2. Italian mould spore suspension is inoculated after radix fici simplicissimatis extractive process citrusfruit wound; 3. Prochloraz positive controls.
Fruit is placed on pallet, puts bag film, and each process 9 fruits, repeat 3 times.Keep relative moisture about 95%, after 27 DEG C of cultivation 7d, investigation citrusfruit lesion diameter, according to following formulae discovery control efficiency.
Control efficiency (%)=100 × (control group lesion diameter-processed group lesion diameter)/control group lesion diameter
Experimental result
1, radix fici simplicissimatis extractive is to the inhibitory action for examination pathogen
Radix fici simplicissimatis extractive to the fungistatic effect of Italian mould, Penicillium digitatum, citric acid maize ear rot bacterium and oranges and tangerines black rot see Fig. 1.Radix fici simplicissimae alcohol extract is respectively 45.6 ± 1.1mm and 41.8 ± 1.0mm to the size of the antibacterial circle diameter of Italian mould and Penicillium digitatum under the concentration of 1g medicinal material/mL, radix fici simplicissimatis extractive is more than 90% to the inhibiting rate of citric acid maize ear rot bacterium under the concentration of 100mg medicinal material/mL, is 100% to the inhibiting rate of black rot.Simultaneously with the Prochlorazs of 1000 times of dilutions for positive control, find that the bacteriostasis of radix fici simplicissimatis extractive is suitable with 1000 times of Prochlorazs diluted.
2, radix fici simplicissimatis extractive suppresses MIC and MFC of Italian mould
Radix fici simplicissimae ethanol extract is respectively 6.25mg radix fici simplicissimae medicinal material/mL and 50mg radix fici simplicissimae medicinal material/mL to Italian mould minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MFC).
3, radix fici simplicissimatis extractive is to the biocontrol effect of south, Jiangxi navel orange
Result is see Fig. 2 and table 1.
Table 1 radix fici simplicissimatis extractive is to the biocontrol effect of south, Jiangxi navel orange
The radix fici simplicissimatis extractive of 100mg medicinal material/mL reaches 67.5% to south, the Jiangxi navel orange disease-controlling effect that Italian mould causes.Show as linear relation between the logarithm value of radix fici simplicissimatis extractive concentration and control efficiency, linear equation is Y=7.3501+1.8261X.EC as calculated
50for 51.6mg/mL.Simultaneously with 500 times of dilutions and 1000 times of Prochlorazs diluted for positive control, find the Prochloraz strong (37.5%) of the control efficiency of 100mg medicinal material/mL radix fici simplicissimatis extractive than 1000 times of dilutions, effect is slightly weaker than the Prochloraz (68.1%) of 500 times of dilutions.
Above-mentioned experimental result all illustrates radix fici simplicissimatis extractive of the present invention, adopts rear multiple pathogen have very strong inhibitory action to oranges and tangerines, effectively can prevent and treat the microbial oranges and tangerines postharvest disease of these cause of diseases.
Embodiment 2 one kinds of radix fici simplicissimatis extractives
Described radix fici simplicissimatis extractive is prepared as follows:
I. by radix fici simplicissimae 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the weight percent concentration adding described radix fici simplicissimae weight 30 times in the powder that step I obtains be 100% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 50 DEG C, and extraction time is 1 hour, and supersonic frequency is 30KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, namely obtains described radix fici simplicissimatis extractive.
The agent of embodiment 3 one kinds of oranges and tangerines post-harvest fresh-keepings
Prescription: radix fici simplicissimatis extractive 100mg (concentration 1g medicinal material/mL) prepared by embodiment 1, carboxymethyl cellulose 10g, potassium citrate 0.6g, sucrose ester 5g, glycerine 5g, calcium propionate 10g, surplus is distilled water, makes 1000mL altogether.
Preparation method: the carboxymethyl cellulose taking recipe quantity, adds the swelling 8h of appropriate distilled water, then adds distilled water stirring preparation cmc soln.Then add citric acid, sucrose ester, glycerine, calcium propionate and radix fici simplicissimatis extractive successively, with distilled water diluting to 1000mL, stir, to obtain final product.
The agent of embodiment 4 one kinds of oranges and tangerines post-harvest fresh-keepings
Prescription: radix fici simplicissimatis extractive 100mg (concentration 1g medicinal material/mL) prepared by embodiment 2, carboxymethyl cellulose 10g, potassium citrate 0.6g, sucrose ester 5g, glycerine 5g, calcium propionate 10g, surplus is distilled water, makes 1000mL altogether.
Preparation method: the carboxymethyl cellulose taking recipe quantity, adds the swelling 8h of appropriate distilled water, then adds distilled water stirring preparation cmc soln.Then add citric acid, sucrose ester, glycerine, calcium propionate and radix fici simplicissimatis extractive successively, with distilled water diluting to 1000mL, stir, to obtain final product.
Embodiment 5-19 radix fici simplicissimatis extractive
Prepare by the following method:
Step I ~ III and embodiment 1 with, but except Extracting temperature, each technological parameter in Step II is all different from embodiment 1, the concrete technological parameter of each embodiment is in table 2.
The process conditions of the ultrasonic extraction of table 2 embodiment 5-19
Test example 2 radix fici simplicissimatis extractive is to the bacteriostasis of oranges and tangerines postharvest pathogenic fungi
Experiment material:
1. supply reagent thing: radix fici simplicissimatis extractive prepared by embodiment 5-19, be mixed with distilled water the solution that concentration is 1g medicinal material/mL.
2. strains tested: Italian mould (Penicillium italicum, ACCC30399).
Experimental technique:
1, agar diffusion method measures radix fici simplicissimatis extractive to the bacteriostasis of Italian mould
With test example 1.
Experimental result: in table 3.
Table 3 radix fici simplicissimatis extractive is to the bacteriostasis of Italian mould
| Embodiment | Antibacterial circle diameter | Embodiment | Antibacterial circle diameter | Embodiment | Antibacterial circle diameter |
| 5 | 32.63±0.82 | 10 | 40.50±0.63 | 15 | 41.83±0.55 |
| 6 | 35.50±1.05 | 11 | 44.00±0.75 | 16 | 44.50±0.98 |
| 7 | 38.67±0.82 | 12 | 46.17±0.82 | 17 | 40.67±0.75 |
| 8 | 43.77±0.98 | 13 | 40.50±0.98 | 18 | 39.83±0.63 |
| 9 | 45.17±0.75 | 14 | 38.53±0.55 | 19 | 37.50±0.63 |
Result of the test shows, extracting factor described in embodiment of the present invention 5-19 extracts the radix fici simplicissimae ethanol extract obtained fungistatic effect, but the strongest with the ethanol extract effect of embodiment 12, being secondly embodiment 9 and 16, is embodiment 8,10,11,13,15 again.Therefore, the preparation method of the preferred a kind of radix fici simplicissimatis extractive of the present invention, comprises the steps:
I. by radix fici simplicissimae 40 ~ 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the mass percent concentration adding described radix fici simplicissimae weight 10 ~ 40 times in the powder that step I obtains be 60% ~ 100% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 40 DEG C ~ 60 DEG C, extraction time is 30min ~ 150min, and supersonic frequency is 30 ~ 40KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, to obtain final product.
The preparation method of the preferred a kind of radix fici simplicissimatis extractive of the present invention, comprises the steps:
I. by radix fici simplicissimae 40 ~ 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the mass percent concentration adding described radix fici simplicissimae weight 20 ~ 40 times in the powder that step I obtains be 90% ~ 100% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 40 DEG C ~ 60 DEG C, extraction time is 60min ~ 90min, and supersonic frequency is 30 ~ 40KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, to obtain final product.
The preparation method of the further preferred radix fici simplicissimatis extractive of the present invention, comprises the steps:
I. by radix fici simplicissimae 40 ~ 50 DEG C oven dry, cross 40 mesh sieves after pulverizing, get minus sieve powder;
II. the mass percent concentration adding described radix fici simplicissimae weight 30 times in the powder that step I obtains be 90% ethanol carry out ultrasonic assistant extraction, Extracting temperature is 60 DEG C, and extraction time is 60min, and supersonic frequency is 30 ~ 40KHz; Filter, obtain alcohol extract;
III. the alcohol extract that obtains of Step II, decompression recycling ethanol, to without alcohol taste, to obtain final product.
In a word, the invention provides and a kind ofly rear multiple pathogen is adopted to oranges and tangerines there is very strong inhibiting radix fici simplicissimatis extractive, effectively can prevent and treat the microbial oranges and tangerines postharvest disease of these cause of diseases.
Claims (9)
1. a preparation method for radix fici simplicissimatis extractive, adopts ultrasonic assistant extractive technique, is Extraction solvent, comprises the steps: with ethanol
Be the ethanol of 60% ~ 100% by the mass percent concentration adding radix fici simplicissimae weight 10 ~ 40 times in described radix fici simplicissimae, be 40 ~ 60 DEG C at Extracting temperature, extraction time is 30min ~ 150min, and supersonic frequency is extract under the condition of 30 ~ 40KHz; Filter, reclaim ethanol, concentrated, to obtain final product.
2. preparation method according to claim 1, is characterized in that: the weight of ethanol is described radix fici simplicissimae weight 20 ~ 40 times, is preferably 30 times;
Preferably, the mass percent concentration of ethanol is 90% ~ 100%, is more preferably 90%;
Preferably, extraction time is 30 ~ 90min, is more preferably 60min.
3. preparation method according to claim 1 and 2, is characterized in that: the temperature that ultrasonic assistant extracts is 60 DEG C.
4. preparation method according to any one of claim 1 to 3, is characterized in that: before alcohol extract, described radix fici simplicissimae 40 ~ 50 DEG C oven dry, crosses 40 mesh sieves, get minus sieve powder and carry out ultrasonic assistant extraction after pulverizing.
5. the radix fici simplicissimatis extractive that the preparation method according to any one of Claims 1-4 prepares.
6. radix fici simplicissimae or radix fici simplicissimatis extractive are for the preparation of the purposes in the pharmaceutical composition of control oranges and tangerines postharvest pathogen and/or the agent of oranges and tangerines post-harvest fresh-keeping; Preferably, described radix fici simplicissimatis extractive is prepared by the preparation method according to any one of Claims 1-4.
7. purposes according to claim 6, it is characterized in that, described oranges and tangerines postharvest pathogen is selected from Italian mould (Penicillium italicum), Penicillium digitatum (Penicillium digitatum), one or more in citric acid maize ear rot bacterium (Geotrichumcandidum) and oranges and tangerines black rot (Alternaria citri).
8. prevent and treat a pharmaceutical composition for oranges and tangerines postharvest pathogen, comprise radix fici simplicissimatis extractive according to claim 5.
9. an oranges and tangerines post-harvest fresh-keeping agent, comprises radix fici simplicissimatis extractive according to claim 5.
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| CN112063684A (en) * | 2020-11-16 | 2020-12-11 | 广州智汇生物科技有限公司 | Method and kit for detecting illegal addition of antibiotics in cosmetics |
| CN115054554A (en) * | 2022-07-20 | 2022-09-16 | 三明学院 | Hispid fig washing-free hand sanitizer and preparation method thereof |
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Cited By (8)
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| CN107232299A (en) * | 2017-06-29 | 2017-10-10 | 中国水产科学研究院黄海水产研究所 | A kind of citrus antimicrobial antistaling agent |
| CN108936277A (en) * | 2018-05-29 | 2018-12-07 | 佛山市三水区嘉信农业技术研究院（普通合伙） | A kind of noodles promoting postpartum milk secretion and recovery |
| CN112063684A (en) * | 2020-11-16 | 2020-12-11 | 广州智汇生物科技有限公司 | Method and kit for detecting illegal addition of antibiotics in cosmetics |
| CN112063684B (en) * | 2020-11-16 | 2021-02-02 | 广州智汇生物科技有限公司 | Method and kit for detecting illegal addition of antibiotics in cosmetics |
| CN115054554A (en) * | 2022-07-20 | 2022-09-16 | 三明学院 | Hispid fig washing-free hand sanitizer and preparation method thereof |
| CN115089629A (en) * | 2022-07-20 | 2022-09-23 | 三明学院 | Freeze-dried hispid fig fruit flavone extracting solution and preparation method thereof |
| CN115089629B (en) * | 2022-07-20 | 2023-09-12 | 三明学院 | Ficus simplicissima lour freeze-dried fruit flavone extracting solution and preparation method thereof |
| CN115054554B (en) * | 2022-07-20 | 2024-03-22 | 三明学院 | Five-finger wild peach no-wash hand cleanser and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN104585244B (en) | 2017-08-29 |
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