CN106071411A - A kind of mixed feed improving shrimp immunity vigor and preparation method thereof - Google Patents
A kind of mixed feed improving shrimp immunity vigor and preparation method thereof Download PDFInfo
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- CN106071411A CN106071411A CN201610529070.5A CN201610529070A CN106071411A CN 106071411 A CN106071411 A CN 106071411A CN 201610529070 A CN201610529070 A CN 201610529070A CN 106071411 A CN106071411 A CN 106071411A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of mixed feed improving shrimp immunity vigor and preparation method thereof, wherein mixed feed includes following raw material: Kelp Powder, bean cake, gluten flour, choline chloride, immune polysaccharide, garlicin, dalcium biphosphate, soybean phospholipid, Vc phosphate ester, maize cob meal, Testa oryzae, fish flour, defat krill meal and fish oil；Its preparation method comprises the following steps: destruction step, batching step, granulation step.The invention has the beneficial effects as follows: containing invigorating spleen and reinforcing stomach, the medicated premix of benefiting vital QI for tranquillizing in the mixed feed of the present invention, shrimp immunity of organisms can be improved, drug residue free, medicated premix cooperates with the raw material of other mixed feeds, interact, disclosure satisfy that the nutritional need of shrimp, improve rate of body weight gain and rate of growth, after this type of Macrobrachium nipponensis edible, also body can be played certain health-care effect.
Description
Technical field
The present invention relates to bird feed, particularly to a kind of mixed feed improving shrimp immunity vigor and preparation method thereof.
Background technology
Along with the fast development of shrimp culture industry and improving constantly of intensive culture degree, disease problem becomes increasingly conspicuous,
In traditional pest control method, antibiotic and chemicals are considered main means and use in a large number in cultivation, lead
The substance that causes a disease produces drug resistance, and therapeutic effect declines；Simultaneously as the residual of medicine, have a strong impact on consumer groups health and
The safety of ecological environment.Cause product water outlet to be obstructed owing to aquatic products drug residue exceeds standard, have a strong impact on China's aquatic products
Competitiveness, hinders the developing of domestic and international market, causes huge economic loss.
Macrobrachium rosenbergii originates in the India Pacific region, lives in various types of fresh water or salt-fresh water waters, the 60's
Since, successively move support in Asia, Europe, some countries and regions such as America.Within 1976, introduce China from Japan, currently mainly
Cultivation is promoted more than 10, south province (city, district), the fastest with Development In Guangdong Province.Macrobrachium rosenbergii every hectogram Macrobrachium nipponensis contains protein 20.6
Gram, 0.7 gram of fat, and containing multivitamin and the necessary trace element of human body, be high-protein nutrient aquatic products.Roche natural pond
Shrimp has that growth is fast, culture-cycle big, economic worth advantages of higher short, individual, is a kind of excellent freshwater aquiculture object.Mesh
Before, Macrobrachium rosenbergii has formed scale cultivation in China, and Macrobrachium rosenbergii industrial seedling rearing is also achieved with important achievement, but children
Often some diseases is there is in the body nurturing period during becoming shrimp aquaculture.Chinese herbal medicine has antibacterial, antiviral, raising immunity of organisms
With improve the effect of growth of animal performance, be effectively reduced the aquaculture that metabolite discharge causes and pollute, and raw material sources are wide, valency
Lattice are cheap, are the feedstuffs of a kind of green, high-efficiency environment friendly, it can be considered to be added medium-height grass in Macrobrachium rosenbergii feedstuff
Medicine, thus reach to improve the purpose of Macrobrachium rosenbergii immunity.
Summary of the invention
The present invention provides a kind of mixed feed improving shrimp immunity vigor and preparation method thereof, in the mixed feed of the present invention
Containing invigorating spleen and reinforcing stomach, the medicated premix of benefiting vital QI for tranquillizing, it is possible to improve shrimp immunity of organisms, drug residue free, medicated premix
Cooperate with the raw material of other mixed feeds, interact, it is possible to meet the nutritional need of shrimp, improve rate of body weight gain and growth
Rate, also can play certain health-care effect after this type of Macrobrachium nipponensis edible to body.
In order to realize foregoing invention purpose, the invention provides a kind of mixed feed improving shrimp immunity vigor, wherein, institute
State feedstuff and include following raw material: Kelp Powder, bean cake, gluten flour, choline chloride, immune polysaccharide, garlicin, dalcium biphosphate,
Soybean phospholipid, Vc phosphate ester, maize cob meal, Testa oryzae, fish flour, defat krill meal and fish oil.
Described mixed feed is made up of the raw material of following parts by weight: Kelp Powder 70-140 weight portion, bean cake 100-300 weight
Amount part, gluten flour 80-160 weight portion, choline chloride 0.5-1 weight portion, immune polysaccharide 0.3-0.7 weight portion, garlicin 5-
10 weight portions, dalcium biphosphate 0.5-1.5 weight portion, soybean phospholipid 0.3-0.7 weight portion, Vc phosphate ester 0.4-0.8 weight
Part, maize cob meal 120-220 weight portion, Testa oryzae 15-30 weight portion, fish flour 20-100 weight portion, defat krill meal 0-70 weight
Part and fish oil 14-28 weight portion.
Described mixed feed is made up of the raw material of following parts by weight: Kelp Powder 110 weight portion, bean cake 220 weight portion, height
Gluten flour 120 weight portion, choline chloride 0.7 weight portion, immune polysaccharide 0.5 weight portion, garlicin 7 weight portion, dalcium biphosphate
1.2 weight portions, soybean phospholipid 0.5 weight portion, Vc phosphate ester 0.6 weight portion, maize cob meal 170 weight portion, Testa oryzae 22 weight
Part, fish flour 70 weight portion, defat krill meal 35 weight portion and fish oil 22 weight portion.
Described mixed feed also includes medicated premix, and described mixed feed is made up of the raw material material of following parts by weight:
Kelp Powder 70-140 weight portion, bean cake 100-300 weight portion, gluten flour 80-160 weight portion, choline chloride 0.5-1 weight
Part, immune polysaccharide 0.3-0.7 weight portion, garlicin 5-10 weight portion, dalcium biphosphate 0.5-1.5 weight portion, soybean phospholipid
0.3-0.7 weight portion, Vc phosphate ester 0.4-0.8 weight portion, maize cob meal 120-220 weight portion, Testa oryzae 15-30 weight portion, fish
Powder 20-100 weight portion, defat krill meal 0-70 weight portion, fish oil 14-28 weight portion and medicated premix 10-30 weight portion.
Described additive for fodder is made up of the raw material of following parts by weight: Kelp Powder 110 weight portion, bean cake 220 weight
Amount part, gluten flour 120 weight portion, choline chloride 0.7 weight portion, immune polysaccharide 0.5 weight portion, garlicin 7 weight portion, phosphoric acid
Calcium dihydrogen 1.2 weight portion, soybean phospholipid 0.5 weight portion, Vc phosphate ester 0.6 weight portion, maize cob meal 170 weight portion, Testa oryzae 22
Weight portion, fish flour 70 weight portion, defat krill meal 35 weight portion, fish oil 22 weight portion and medicated premix 20 weight portion.
Described medicated premix is made up of the raw material of following parts by weight: Radix potentillae anserinae 10-20 weight portion, Herba Pterocephali bretschneideri 12-
32 weight portions, Radix Notoginseng 12-24 weight portion, Ganoderma 15-35 weight portion, Flos Lonicerae 13-27 weight portion, Radix Pseudostellariae 14-32 weight portion,
Folium Brassicae caulorapae 11-21 weight portion, Herba Orchidis Latifoliae 15-25 weight portion, rub Bai Ye 11-27 weight portion, Rhizoma et radix valerianae 13-34 weight portion, Herba Lobeliae Chinensis
12-22 weight portion, Fructum Artemisiae Argyi 14-28 weight portion, Rhizoma Tupistrae Chinensis 12-22 weight portion and Salix chienii Cheng 13-25 weight portion.
Described medicated premix is made up of the raw material of following parts by weight: Radix potentillae anserinae 14 weight portion, Herba Pterocephali bretschneideri 26 weight
Part, Radix Notoginseng 15 weight portion, Ganoderma 23 weight portion, Flos Lonicerae 18 weight portion, Radix Pseudostellariae 21 weight portion, Folium Brassicae caulorapae 15 weight portion, red
Door blue 19 weight portions, rub Bai Ye 25 weight portion, Rhizoma et radix valerianae 33 weight portion, Herba Lobeliae Chinensis 16 weight portion, Fructum Artemisiae Argyi 23 weight portion, Rhizoma Tupistrae Chinensis 14
Weight portion and Salix chienii Cheng 22 weight portion.
The preparation method of described medicated premix comprises the following steps:
The first step, is mixed in proportion described each raw material, and adding relative to the determining alcohol of mixture quality 2～4 times is 85～95%
Ethanol, be heated to reflux 1～3 hour, extract, filter obtain the first extracting solution；Filter the medicinal residues obtained to add relative to institute
State the ethanol that determining alcohol is 85～95% of medicinal residues quality 1～2 times, be heated to reflux 1～3 hour, extract, filter acquisition second and carry
Take liquid；First extracting solution and the second extracting solution being merged, concentrating under reduced pressure removes alcohol solvent, is dried, it is thus achieved that dry cream；
Second step, is placed in super micron mill pulverizing 1～2 hour by the dry cream that the first step obtains, and pulverizes, sieves, it is thus achieved that 300
Mesh～the micropowders of 400 mesh, it is thus achieved that medicated premix powder.
In order to preferably realize foregoing invention purpose, the invention provides a kind of mixed feed improving shrimp immunity vigor
Preparation method, described preparation method comprises the following steps:
(1) destruction step: weigh bean cake, Testa oryzae by ratio of weight and the number of copies, puts into pulverizer and carries out Crushing of Ultrafine, mistake after pulverizing
2.0mm sieve；
(2) batching step: weigh Kelp Powder, gluten flour, choline chloride, immune polysaccharide, garlicin, phosphorus according to ratio of weight and number
Acid dihydride calcium, soybean phospholipid, Vc phosphate ester, maize cob meal, fish flour, defat krill meal, fish oil and medicated premix, with step
(1) pulverizing the fine powder mixing obtained, put into mixer, mix homogeneously, incorporation time is 30-60s；
(3) granulation step: putting into granulator and pelletize, pelleting temperature is 85 DEG C so that it is gelatinization degree reaches more than 90%.
Described defat krill meal is the defat krill meal that Wudi Yu Jin commerce and trade company limited produces, wherein, defat krill meal
Crude protein content be 68%, fat content is 6%.
The invention has the beneficial effects as follows: in the mixed feed of the present invention, the medicine containing invigorating spleen and reinforcing stomach, benefiting vital QI for tranquillizing adds
Agent, it is possible to improve shrimp immunity of organisms, drug residue free, medicated premix cooperates with the raw material of other mixed feeds, mutually
Phase separation, it is possible to meet the nutritional need of shrimp, improves rate of body weight gain and rate of growth, also body can be played one after this type of Macrobrachium nipponensis edible
Determine health-care effect.
Detailed description of the invention
The invention provides a kind of mixed feed improving shrimp immunity vigor and preparation method thereof, including medicated premix,
Medicated premix includes following raw material: Radix potentillae anserinae, Herba Pterocephali bretschneideri, Radix Notoginseng, Ganoderma, Flos Lonicerae, Radix Pseudostellariae, Folium Brassicae caulorapae, Herba Orchidis Latifoliae,
Rub Bai Ye, Rhizoma et radix valerianae, Herba Lobeliae Chinensis, Fructum Artemisiae Argyi, Rhizoma Tupistrae Chinensis and Salix chienii Cheng.
The pharmacological property of above-mentioned each raw material is as follows:
Semen Ziziphi Spinosae: another name: Fructus Choerospondiatis core, behenic acid Fructus Jujubae.Source: this product is Rhamnaceae plant Ziziphi Spinosae Ziziphus jujuba Mill.
Var. the dry mature seed of spinosa (Bunge) Hu ex H.F.Chou.Time of the year when autumn changes into winter gathers mature fruit, removes fruit
Meat and nucleocapsid, collect seed, dry.Nature and flavor: sweet, sour are flat.Gui Jing: return liver, gallbladder, heart channel.Cure mainly: tonifying liver, mind calming, arresting sweating, raw
Tianjin.For restlessness of asrhenia type and insomnia, dreaminess of palpitating with fear, body void hyperhidrosis, Tianjin wound is thirsty.
Fructum Artemisiae Argyi: another name: argy wormwood (" Treatise on Dietetic Therapy ").Source: " property of medicine opinion ".Source: for the fruit of feverfew Chinese mugwort.9～
In October, gather after fruit maturation.Nature and flavor: 1. " Japan hanako materia medica): " warm, nontoxic." 2. " detailed outline ": " toil, heat, nontoxic." main
Control: 1. " property of medicine opinion ": " main improving eyesight." 2. " Japan hanako materia medica ": " tonifying YANG, helps Shui Zang, (sharp) waist, knee joint and warm uterus."
Herba Lobeliae Chinensis: another name: Herba Lobeliae Chinensis, anxious solution rope, half of flower, Magnoliacoco [Guangdong], long Cordyceps [Henan].Source: this product is Fructus Citri tangerinae
The dry herb of stalk section plant Herba Lobeliae Chinensis Lobelia chinensis Lour..Summer gathers, and removes silt, cleans, dries.
Nature and flavor: pungent are flat.Gui Jing: GUIXIN, small intestinal, lung meridian.Cure mainly: inducing diuresis to remove edema, heat-clearing and toxic substances removing.
Salix chienii Cheng: source: Salicaceae Salix chienii Cheng Salix chienii Cheng, is used as medicine with root.Cure mainly: heat-clearing and toxic substances removing.
Rub Bai Ye: another name: powder Folium paulowniae, sheep gristle, sleep fruit, Canis familiaris L. rib bar.Source: Caprifoliaceae pod platymiscium Viburnum cylindricum
Viburnum cylindricum Buch.-Ham. ex D. Don, is used as medicine with root, leaf, flower.Root, leaf can be adopted the whole year, dry；
Hua Xiaqiu gathers.Nature and flavor: bitter are cool.Cure mainly: leaf: heat-clearing and toxic substances removing.
Folium Brassicae caulorapae: source: " the southern regions of the Yunnan Province book on Chinese herbal medicine ".Source: for the leaf of crucifer kohlrabi.Cure mainly: control food stagnation, expectorant
Long-pending, malignant boil.1. " the southern regions of the Yunnan Province book on Chinese herbal medicine ": " apply malignant boil." 2. " agricultural administration book ": " Ye Jizi: help digestion long-pending, solves face poison."
Flos Lonicerae: another name: Flos Lonicerae, Flos Lonicerae, Flos Lonicerae, two precious flowers.Source: this product is caprifoliaceae plant Radix Ophiopogonis Lonicera
Japonica Thunb., Flos Lonicerae Lonicera hypoglauca Miq., Flos Lonicerae (hair calyx Radix Ophiopogonis) Lonicera
The flower that confusa DC. or the dry flower of hair style Radix Ophiopogonis Lonicera dasystyla Rehd. or band just open.Early summer flower
Gather before opening, be dried.Nature and flavor: sweet, cold.Gui Jing: return lung, the heart, stomach warp.Cure mainly: heat-clearing and toxic substances removing, wind-heat dissipating.For carbuncle
Furuncle, sore throat, erysipelas, toxic-heat and blood stasis, anemopyretic cold, epidemic febrile disease is generated heat.
Herba Pterocephali bretschneideri: another name: rock seven [Xichang Sichuan], climb rock sunset [can manage], Herba Spilanthis callimorphae's [in wood].Source: Radix Dipsaci
Section wing head flower plant Herba Pterocephali bretschneideri Pterocephalus bretschneideri (Batal.) Pritz., with root and complete
Grass is used as medicine.Cure mainly: expelling wind and removing dampness, blood circulation promoting and blood stasis dispelling, pain relieving.
Radix Notoginseng: source: this product is the dry of panax araliaceae plant Panax notoginseng (Burk.) F. H. Chen
Dry.Autumn excavates before the flowers are in blossom, cleans, separately main root, supporting root and stem foot, is dried.Supporting root is practised and is claimed " rib ", and stem foot is practised and claimed " to cut
Mouthful ".Nature and flavor: hardship sweet, micro-, temperature.Gui Jing: return liver, stomach warp.Cure mainly: dissipating blood stasis stops blooding, subduing swelling and relieving pain.For spitting blood, spit blood, epistaxis,
Having blood in stool, metrorrhagia, traumatic hemorrhage, chest and abdomen twinge, tumbling and swelling.
Rhizoma et radix valerianae: another name: Rhizoma et radix valerianae, little rescue [Shaanxi], deer grass, Herba rutae.Source: Valerianaceae valerian Rhizoma et radix valerianae
Valeriana officinalis L. and Valeriana amurensis V. amurensis P. Smirn., is used as medicine with root stock and root.Autumn
Season gathers, and removes rice shoot and earth, dries.Nature and flavor: acrid, sweet, warm.Cure mainly: calm the nerves, regulate the flow of vital energy, pain relieving.For neurasthenia, lose
Sleep, hysteria, epilepsy, stomach abdominal distention and pain, lumbago and skelalgia, traumatic injury.
Radix Pseudostellariae: another name: Radix Ginseng, double batches seven, pseudostellaria heterophylla.Source: this product is pinkwort Radix Ginseng
The dried root of Pseudostellaria heterophylla (Miq.) Pax ex Pax et Hoffm..Summer, stem and leaf was big
Part is excavated time withered, cleans, and removes fibrous root, puts and dries or directly dry after slightly scalding in boiling water.Nature and flavor: hardship sweet, micro-are flat.Return
Warp: return spleen, lung meridian.Cure mainly: replenishing QI to invigorate the spleen, promoting the production of body fluid for nourishing the lung.Tired for insufficiency of the spleen body, inappetence, weak after being ill, deficiency of both vital energy and Yin,
Spontaneous perspiration is thirsty, dryness of the lung dry cough.
Rhizoma Tupistrae Chinensis: another name: bamboo root ginseng, Rhizoma Tupistrae ensifoliae, oxtail seven (" Shaanxi Chinese herbal medicine ").Source: " Shaanxi Chinese herbal medicine ".Come
Source: for the rhizome of liliaceous plant Tupistra chinensis Bak..The whole year can adopt. and remove fibrous root and leaf, take rhizome and dry.Nature and flavor: " Shaanxi medium-height grass
Medicine ": " sweet and slightly bitter taste, cold in nature." cure mainly: nourishing yin and purging fire, promoting blood flow to regulate menstruation.Control consumptive fever cough, rheumatic arthralgia, menoxenia, traumatic injury
Damage.1. " Shaanxi Chinese herbal medicine ": " except rheumatism, clearing away heat-fire, pain easing and hemostasis, regulating menstruation and activating blood, YIN nourishing qi-restoratives.Treating rheumatic joint
Inflammation, lumbago and skelalgia, traumatic injury, impairment caused by overstrain, menoxenia, hectic fever due to YIN-deficiency consumptive fever." 2. " Chengdu Chinese herbal medicine ": " benefiting QI for activating blood circulation, lung heat clearing, solve
Poison."
Ganoderma: another name: Ganoderma lucidum seu Japonicum, Ganoderma, Ganoderma Lucidum.Source: this product is On Polyporaceae Ganoderma lucidum (Leyss. Ex Fr.) Karst. Ganoderma
Lucidum(Leyss.ex Fr.) the dry son of Karst. or Ganoderma Ganoderma sinense Zhao, Xu et Zhang is real
Body.The whole year gathers, and removes impurity, wipes out the lower end stem with rotten wood, silt or culture matrix, dry in the shade or 40～50 DEG C of bakings
Dry.Nature and flavor: sweet are flat.Gui Jing: GUIXIN, lung, liver, kidney channel.Cure mainly: invigorating QI and tranquilization, relieving cough and asthma.For dizziness sleeplessness, cardiopalmus gas
Short, asthenia cough with asthma.
Herba Orchidis Latifoliae: another name: wide leaf Herba Orchidis Latifoliae.Source: the orchid family Herba Orchidis Latifoliae Orchis latifolia L.(L.salina
Turcz.), with all herbal medicine.Nature and flavor: sweet are flat.Cure mainly: heart tonifying, the kidney invigorating, promote the production of body fluid, quench the thirst, strengthening the spleen and stomach.
Radix potentillae anserinae: another name: Herba Herminii, Rhizoma Nelumbinis flower, Radix potentillae anserinae.Source: Rosaceae potentilla plants Potentilla anserina L.
Potentilla anserina L., is used as medicine with tuber.Summer excavates, and cleans and dries.Nature and flavor: sweet are flat.Cure mainly: fill blood, strong
Taste, promoting the production of body fluid to quench thirst, dampness removing.For anemia after being ill, malnutrition, insufficiency of the spleen diarrhoea, rheumatic arthralgia.
Embodiment is below used to describe embodiments of the present invention in detail, whereby to the present invention how application technology means
Solve technical problem, and the process that realizes reaching technique effect can fully understand and implement according to this.
Embodiment 1 medicated premix
Take Radix potentillae anserinae 14 weight portion, Herba Pterocephali bretschneideri 26 weight portion, Radix Notoginseng 15 weight portion, Ganoderma 23 weight portion, Flos Lonicerae 18 weight
Part, Radix Pseudostellariae 21 weight portion, Folium Brassicae caulorapae 15 weight portion, Herba Orchidis Latifoliae 19 weight portion, rub Bai Ye 25 weight portion, Rhizoma et radix valerianae 33 weight portion,
Herba Lobeliae Chinensis 16 weight portion, Fructum Artemisiae Argyi 23 weight portion, Rhizoma Tupistrae Chinensis 14 weight portion and Salix chienii Cheng 22 weight portion.
Preparation method comprises the following steps:
The first step, is mixed in proportion described each raw material, and adding relative to the determining alcohol of mixture quality 2～4 times is 85～95%
Ethanol, be heated to reflux 1～3 hour, extract, filter obtain the first extracting solution；Filter the medicinal residues obtained to add relative to institute
State the ethanol that determining alcohol is 85～95% of medicinal residues quality 1～2 times, be heated to reflux 1～3 hour, extract, filter acquisition second and carry
Take liquid；First extracting solution and the second extracting solution being merged, concentrating under reduced pressure removes alcohol solvent, is dried, it is thus achieved that dry cream；
Second step, is placed in super micron mill pulverizing 1～2 hour by the dry cream that the first step obtains, and pulverizes, sieves, it is thus achieved that 300
Mesh～the micropowders of 400 mesh, it is thus achieved that medicated premix powder.
Embodiment 2 feedstuff
Embodiments provide a kind of mixed feed improving shrimp immunity vigor and preparation method thereof, each raw material in feedstuff
Parts by weight be: Kelp Powder 110 weight portion, bean cake 220 weight portion, gluten flour 120 weight portion, choline chloride 0.7 weight
Part, immune polysaccharide 0.5 weight portion, garlicin 7 weight portion, dalcium biphosphate 1.2 weight portion, soybean phospholipid 0.5 weight portion, Vc
Phosphate ester 0.6 weight portion, maize cob meal 170 weight portion, Testa oryzae 22 weight portion, fish flour 70 weight portion, defat krill meal 35 weight
Part, fish oil 22 weight portion；Wherein medicated premix is the medicated premix powder of embodiment 1.
Its preparation method comprises the following steps:
(1) destruction step: weigh bean cake, Testa oryzae by ratio of weight and the number of copies, puts into pulverizer and carries out Crushing of Ultrafine, mistake after pulverizing
2.0mm sieve；
(2) batching step: weigh Kelp Powder, gluten flour, choline chloride, immune polysaccharide, garlicin, phosphorus according to ratio of weight and number
Acid dihydride calcium, soybean phospholipid, Vc phosphate ester, maize cob meal, fish flour, defat krill meal, fish oil and medicated premix, with step
(1) pulverizing the fine powder mixing obtained, put into mixer, mix homogeneously, incorporation time is 40s；
(3) granulation step: putting into granulator and pelletize, pelleting temperature is 85 DEG C so that it is gelatinization degree reaches more than 90%.
The toxicity test of medicated premix:
1, trial drug
Choose Radix potentillae anserinae 14g, Herba Pterocephali bretschneideri 26g, Radix Notoginseng 15g, Ganoderma 23g, Flos Lonicerae 18g, Radix Pseudostellariae 21g, Folium Brassicae caulorapae 15g,
Herba Orchidis Latifoliae 19g, rub Bai Ye 25g, Rhizoma et radix valerianae 33g, Herba Lobeliae Chinensis 16g, Fructum Artemisiae Argyi 23g, Rhizoma Tupistrae Chinensis 14g and Salix chienii Cheng 22g mixing, be ground into
Powder；To obtain powder and add immersion 25 hours in 140g cold water, with slow fire boiling, decocting time is 1.5 hours, by decoction liquor
Filter, it is thus achieved that the first filtrate, collect filtering residue；Again add in 140g cold water by filtering the filtering residue obtained, with slow fire boiling 3 times,
Decoct 1 hour every time, filter and merge filtrate, it is thus achieved that the second filtrate；Merging twice filtrate, concentrating under reduced pressure removes part water,
Obtaining decoction, the crude drug concentration of decoction is 3g/ml.
2, test method: select SD rat, gives the medicine of the present invention of variable concentrations (18.0,6.0,2.g crude drug/kg)
The decoction of additive, every day gavage once, continuous 90 days, last be administered latter 24 hours respectively group live and kill 1/2 animal (male and female half and half),
Remaining 1/2 animal lives after continuing to observe 2 weeks and kills.The outward appearance of animal, general behavior, food ration, body weight change is observed during test
Change, be administered latter 90 days and drug withdrawal carries out hematology's (RBC, HB, reticulocyte, PLT, CT, WBC and classification) for 2 weeks and blood is raw
Change (AST, ALT, ALP, Glu, BUN, Crca, TP, T.BIL, ALB, CHOL), urine biochemistry, organ coefficient, histopathology
Deng index checking.Result of the test shows: the medicated premix of the present invention is good at high, medium and low dosage treated animal general state, outward
See sign, behavioral activity, food-intake and body weight and increase change the most without exception；Three dosage groups and matched group hematological examination, blood
Liquid is biochemical, urine biochemical analysis all between normal range, group without significant difference；Each group main organs histopathological examination is not
See obvious exception.These parameters drug withdrawal also had no change after 2 weeks.This test dosage be respectively clinical medicine dose 180,
60,20 times, according to the Chinese medicine preparation of the result of the test present invention in high, medium and low three dosage (18.0,6.0,2.g crude drug/kg)
Rat is had no significant effect by administration in continuous 90 days, and without clear and definite toxicity target organ and sensitive indicator, convalescent period observation also has no and prolongs
Property toxic reaction late, the dose safety of the pharmaceutical preparation clinical practice of the prompting present invention is higher.
Contrast test
Want Macrobrachium rosenbergii field to choose the Macrobrachium rosenbergii of 600 urosome weight 2g from Guangdong height, from above-mentioned Macrobrachium rosenbergii, randomly select 400
Tail Macrobrachium rosenbergii is divided into embodiment 1 group and matched group, often organizes 200 tails, and often group sets 5 repetitions, each repetition 40 tail；In indoor two
In individual cement pit, packet is carried out, and cultivation water filters after precipitation, keeps continuous charge, and in breeding process, water temperature is 26.5-27.5
DEG C, salinity 28%；Embodiment 1 group and matched group use the feedstuff of the embodiment of the present invention 2 and conventional feed to feed Roche natural pond respectively
Shrimp, often group is according to above-mentioned corresponding feedstuff of throwing something and feeding；Manually be satiated with food every day throw something and feed four times (8:00,11:00,14:00,17:00), often
10 feedstuffs of secondary input, wait to rob food complete, continue to put into 10, circulate successively, and management is supported 50 days temporarily routinely, carries out daily
The rate of body weight gain (%) of Macrobrachium rosenbergii, survival rate (%) and shrimp blood cell count, cell phagocytic rate, hemocyte bacteriolyze, antibacterial activity
Experimental result record is respectively such as following table:
Experimental result uses average ± standard deviation to represent, applies one factor analysis of variance (ANOVA) statistical data, works as ANOVA
When display exists significant difference between processing, application Duncan ' s multiple comparisons is to analyze.All statistical analysiss use SPSS11.5
Statistical software, with P < 0.05 as the significance of difference.
Table 1 is the impact on Macrobrachium rosenbergii growth performance of the feedstuff of each experimental group:
| Group | Matched group | Embodiment 1 group |
| Survival rate (%) | 91.47±1.22<sup>a</sup> | 100±0.00 |
| Rate of body weight gain (%) | 147.87±3.38<sup>a</sup> | 161.31±2.30<sup>b</sup> |
Note: normal data, colleague's shoulder note same letter represents that difference is not notable (P ＞ 0.05), and shoulder is noted different letter person and represented poor
Different notable (P ＜ 0.05).
Knowable to the data of table 1: compared with matched group, the survival rate of the Macrobrachium rosenbergii that the embodiment of the present invention is 1 group, rate of body weight gain
Exceeding matched group 8.53% and 13.44% respectively, therefore, the feedstuff of the present invention can improve Macrobrachium rosenbergii rate of body weight gain and rate of growth,
Meet Macrobrachium rosenbergii, nutritional need.
After experiment terminates, from each cement pit, take shrimp 10 tail, extract shrimp haemolymph, carry out immune indexes analysis.
Immune indexes hemocyte quantitative measurement: hemocyte quantitative measurement uses blood counting chamber aobvious in Olympus difference
The method directly counted under micro mirror, takes the mixing of anticoagulation lymph sample and is placed on blood counting chamber, observe, count total hemocyte
(Total haemocyte cells, THC) quantity.
Hemocyte phagocytic rate measures: experiment pathogen used is vibrio alginolyticus (Vibrio alginolyticus), by bacterium
Kind be inoculated on 2116E solid medium, at 28 DEG C, cultivate 12-24h, then with the Litopenaeus vannamei normal saline of sterilizing from
On solid medium, bacterium is washed lower as substrate, centrifugal, resuspended and be made into the bacteria suspension of concentration about 108cells mL-1.Take
600 μ L hemolymph samples, after centrifugal (4 DEG C, 500 × g) 10min, scrubbed, resuspended, be made into 107cells/mL hemocyte
Suspension.Take blood cell suspension 100 μ Ls each with bacteria suspension to mix in addition aseptic plastic shrinkage pool plate, be placed in magazine interior at 25 DEG C
Hatch 30min, then take a mixed liquor and be placed on microscope slide, through drying at room temperature, Giemsa staining, neutral gum mounting (often
Individual sample makees 3 smears), under Olympus optical microscope (oil mirror), observe the blood cell count of counting phagocytosis pathogen
Amount, 200 hemocytees of random counter, employing below equation calculating hemocyte phagocytic rate:
Phagocytic rate (PR)=(phagocytosis vibrio blood cell number/200 hemocyte) × 100%
Antibacterial (Ua), bacteriolyze (UL) vitality test: respectively with Vibrio anguillarum (Vibrio anguillarum) bacteria suspension and molten wall
Micrococcus luteus (Micrococcus lysoleikticus) lyophilized powder is substrate, with the potassium phosphate buffer of sterilizing
(0.1mol L-1, pH=6.3) is made into certain density bacteria suspension (O.D.570nm ≈ 0.3) and takes 3mL and use
The bacteria suspension that 0.1mol/L, pH=6.4 potassium phosphate buffer are made into is placed in ice bath, adds 50 μ L test plasma, in
Survey A0 value at 610nm wavelength, then move in 37 DEG C of water-baths and hatch 30min, be immediately placed on 10min in ice bath after taking-up whole
Only reaction, surveys its A value.
Table 2 is that each experimental group Macrobrachium rosenbergii immunity preparation is analyzed:
| Group | Matched group | Embodiment 1 group |
| Total hemocytes count (individual/ml) | 3.32*10<sup>7a</sup> | 4.67*10<sup>7b</sup> |
| Phagocytic rate (%) | 31<sup>a</sup> | 49<sup>b</sup> |
| Bacteriolytic activity (Units) | 0.007<sup>a</sup> | 0.029<sup>b</sup> |
| Antibacterial activity (Units) | 0.16<sup>a</sup> | 0.31<sup>b</sup> |
Knowable to the data of table 2: compared with matched group, the blood cell count of the Macrobrachium rosenbergii that the embodiment of the present invention is 1 group, cell swallows
Rate, hemocyte bacteriolyze, antibacterial activity are apparently higher than matched group, and therefore, the mixed feed of the present invention can improve shrimp immunity of organism
Vigor.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and
Within principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (9)
1. the mixed feed improving shrimp immunity vigor, it is characterised in that described mixed feed includes following raw material: Thallus Laminariae (Thallus Eckloniae)
Powder, bean cake, gluten flour, choline chloride, immune polysaccharide, garlicin, dalcium biphosphate, soybean phospholipid, Vc phosphate ester, Semen Maydis
Core powder, Testa oryzae, fish flour, defat krill meal and fish oil.
The mixed feed improving shrimp immunity vigor the most according to claim 1, it is characterised in that described mixed feed by with
The raw material of lower parts by weight is made: Kelp Powder 70-140 weight portion, bean cake 100-300 weight portion, gluten flour 80-160 weight
Part, choline chloride 0.5-1 weight portion, immune polysaccharide 0.3-0.7 weight portion, garlicin 5-10 weight portion, dalcium biphosphate 0.5-
1.5 weight portions, soybean phospholipid 0.3-0.7 weight portion, Vc phosphate ester 0.4-0.8 weight portion, maize cob meal 120-220 weight portion,
Testa oryzae 15-30 weight portion, fish flour 20-100 weight portion, defat krill meal 0-70 weight portion and fish oil 14-28 weight portion.
The mixed feed improving shrimp immunity vigor the most according to claim 1 and 2, it is characterised in that described mixed feed
It is made up of the raw material of following parts by weight: Kelp Powder 110 weight portion, bean cake 220 weight portion, gluten flour 120 weight portion, chlorination
Choline 0.7 weight portion, immune polysaccharide 0.5 weight portion, garlicin 7 weight portion, dalcium biphosphate 1.2 weight portion, soybean phospholipid 0.5
Weight portion, Vc phosphate ester 0.6 weight portion, maize cob meal 170 weight portion, Testa oryzae 22 weight portion, fish flour 70 weight portion, defat phosphorus
Shrimp meal 35 weight portion and fish oil 22 weight portion.
4. according to the mixed feed improving shrimp immunity vigor described in any one of claim 1-3, it is characterised in that described cooperation
Feedstuff also includes medicated premix, and described mixed feed is made up of the raw material material of following parts by weight: Kelp Powder 70-140 weight
Part, bean cake 100-300 weight portion, gluten flour 80-160 weight portion, choline chloride 0.5-1 weight portion, immune polysaccharide 0.3-0.7
Weight portion, garlicin 5-10 weight portion, dalcium biphosphate 0.5-1.5 weight portion, soybean phospholipid 0.3-0.7 weight portion, Vc phosphoric acid
Fat 0.4-0.8 weight portion, maize cob meal 120-220 weight portion, Testa oryzae 15-30 weight portion, fish flour 20-100 weight portion, defat phosphorus
Shrimp meal 0-70 weight portion, fish oil 14-28 weight portion and medicated premix 10-30 weight portion.
5. according to the mixed feed improving shrimp immunity vigor described in any one of claim 1-4, it is characterised in that described cooperation
Feed additive is made up of the raw material of following parts by weight: Kelp Powder 110 weight portion, bean cake 220 weight portion, gluten flour 120
Weight portion, choline chloride 0.7 weight portion, immune polysaccharide 0.5 weight portion, garlicin 7 weight portion, dalcium biphosphate 1.2 weight portion,
Soybean phospholipid 0.5 weight portion, Vc phosphate ester 0.6 weight portion, maize cob meal 170 weight portion, Testa oryzae 22 weight portion, fish flour 70 weight
Amount part, defat krill meal 35 weight portion, fish oil 22 weight portion and medicated premix 20 weight portion.
6. according to the mixed feed improving shrimp immunity vigor described in any one of claim 1-5, it is characterised in that described medicine
Additive is made up of the raw material of following parts by weight: Radix potentillae anserinae 10-20 weight portion, Herba Pterocephali bretschneideri 12-32 weight portion, Radix Notoginseng 12-
24 weight portions, Ganoderma 15-35 weight portion, Flos Lonicerae 13-27 weight portion, Radix Pseudostellariae 14-32 weight portion, Folium Brassicae caulorapae 11-21 weight
Part, Herba Orchidis Latifoliae 15-25 weight portion, rub Bai Ye 11-27 weight portion, Rhizoma et radix valerianae 13-34 weight portion, Herba Lobeliae Chinensis 12-22 weight portion, Fructum Artemisiae Argyi
14-28 weight portion, Rhizoma Tupistrae Chinensis 12-22 weight portion and Salix chienii Cheng 13-25 weight portion.
7. according to the mixed feed improving shrimp immunity vigor described in any one of claim 1-6, it is characterised in that described medicine
Additive is made up of the raw material of following parts by weight: Radix potentillae anserinae 14 weight portion, Herba Pterocephali bretschneideri 26 weight portion, Radix Notoginseng 15 weight portion,
Ganoderma 23 weight portion, Flos Lonicerae 18 weight portion, Radix Pseudostellariae 21 weight portion, Folium Brassicae caulorapae 15 weight portion, Herba Orchidis Latifoliae 19 weight portion, rub white
Leaf 25 weight portion, Rhizoma et radix valerianae 33 weight portion, Herba Lobeliae Chinensis 16 weight portion, Fructum Artemisiae Argyi 23 weight portion, Rhizoma Tupistrae Chinensis 14 weight portion and Salix chienii Cheng 22
Weight portion.
8. according to the mixed feed improving shrimp immunity vigor described in any one of claim 1-7, it is characterised in that described medicine
The preparation method of additive comprises the following steps:
The first step, is mixed in proportion described each raw material, and adding relative to the determining alcohol of mixture quality 2～4 times is 85～95%
Ethanol, be heated to reflux 1～3 hour, extract, filter obtain the first extracting solution；Filter the medicinal residues obtained to add relative to institute
State the ethanol that determining alcohol is 85～95% of medicinal residues quality 1～2 times, be heated to reflux 1～3 hour, extract, filter acquisition second and carry
Take liquid；First extracting solution and the second extracting solution being merged, concentrating under reduced pressure removes alcohol solvent, is dried, it is thus achieved that dry cream；
Second step, is placed in super micron mill pulverizing 1～2 hour by the dry cream that the first step obtains, and pulverizes, sieves, it is thus achieved that 300
Mesh～the micropowders of 400 mesh, it is thus achieved that medicated premix powder.
9., according to the preparation method of the mixed feed improving shrimp immunity vigor described in any one of claim 1-8, its feature exists
In, described preparation method comprises the following steps:
(1) destruction step: weigh bean cake, Testa oryzae by ratio of weight and the number of copies, puts into pulverizer and carries out Crushing of Ultrafine, mistake after pulverizing
2.0mm sieve；
(2) batching step: weigh Kelp Powder, gluten flour, choline chloride, immune polysaccharide, garlicin, phosphorus according to ratio of weight and number
Acid dihydride calcium, soybean phospholipid, Vc phosphate ester, maize cob meal, fish flour, defat krill meal, fish oil and medicated premix, with step
(1) pulverizing the fine powder mixing obtained, put into mixer, mix homogeneously, incorporation time is 30-60s；
(3) granulation step: putting into granulator and pelletize, pelleting temperature is 85 DEG C so that it is gelatinization degree reaches more than 90%.
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Cited By (3)
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|---|---|---|---|---|
| CN107821865A (en) * | 2017-11-21 | 2018-03-23 | 集美大学 | A kind of litopenaeus vannamei compound feed of zero fish meal and preparation method thereof |
| CN107950755A (en) * | 2017-11-23 | 2018-04-24 | 安徽金牧饲料有限公司 | A kind of organic shrimp feed of environmental protection and preparation method thereof |
| CN110419477A (en) * | 2019-09-02 | 2019-11-08 | 苏州市毛氏阳澄湖水产发展有限公司 | A kind of cultural method of Macrobrachium rosenbergii seedling |
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| CN103082159A (en) * | 2013-03-07 | 2013-05-08 | 湛江东腾饲料有限公司 | Low-carbon type penaeus monodon compound feed |
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| CN105639095A (en) * | 2016-01-12 | 2016-06-08 | 湛江粤华水产饲料有限公司 | Compound feed capable of improving growth immunity of prawns and preparation method of compound feed |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102783563A (en) * | 2012-08-21 | 2012-11-21 | 江苏省海洋水产研究所 | Environment-friendly feed for exopalaemon carinicauda |
| CN103082159A (en) * | 2013-03-07 | 2013-05-08 | 湛江东腾饲料有限公司 | Low-carbon type penaeus monodon compound feed |
| CN105124283A (en) * | 2015-09-09 | 2015-12-09 | 广西桂林生物动力环保科技有限公司 | Chinese herbal medicine shrimp feed and preparation method thereof |
| CN105639095A (en) * | 2016-01-12 | 2016-06-08 | 湛江粤华水产饲料有限公司 | Compound feed capable of improving growth immunity of prawns and preparation method of compound feed |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107821865A (en) * | 2017-11-21 | 2018-03-23 | 集美大学 | A kind of litopenaeus vannamei compound feed of zero fish meal and preparation method thereof |
| CN107950755A (en) * | 2017-11-23 | 2018-04-24 | 安徽金牧饲料有限公司 | A kind of organic shrimp feed of environmental protection and preparation method thereof |
| CN110419477A (en) * | 2019-09-02 | 2019-11-08 | 苏州市毛氏阳澄湖水产发展有限公司 | A kind of cultural method of Macrobrachium rosenbergii seedling |
| CN110419477B (en) * | 2019-09-02 | 2021-09-17 | 苏州市毛氏阳澄湖水产发展有限公司 | Culture method of macrobrachium rosenbergii seedlings |
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Application publication date: 20161109 |