CN110419477B - Culture method of macrobrachium rosenbergii seedlings - Google Patents
Culture method of macrobrachium rosenbergii seedlings Download PDFInfo
- Publication number
- CN110419477B CN110419477B CN201910824693.9A CN201910824693A CN110419477B CN 110419477 B CN110419477 B CN 110419477B CN 201910824693 A CN201910824693 A CN 201910824693A CN 110419477 B CN110419477 B CN 110419477B
- Authority
- CN
- China
- Prior art keywords
- parts
- water
- seedlings
- pond
- macrobrachium rosenbergii
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention relates to a culture method of macrobrachium rosenbergii seedlings, which is characterized by comprising the following steps: step S1, preparing a seedling culture pond, step S2, stocking seedlings, step S3, feeding baits, and step S4, and performing daily management. Wherein the water depth of the seedling culture pond is 0.5-0.7 m; the stocking density is 2000-4000 tails per square meter; the dissolved oxygen amount is 5.5-5.8 mg/L. The culture method of the macrobrachium rosenbergii larvae can obviously improve the adaptability of the larvae to fresh water and improve the survival rate of the macrobrachium rosenbergii by synchronously adjusting the salinity, the temperature and the bait in the culture process of the larvae, so that the growth speed of the larvae is accelerated, the feeding speed is high, the stability is good, the nutrition is rich, the immunity is enhanced, and the method is convenient and easy to implement and has low cost.
Description
Technical Field
The invention belongs to the technical field of aquatic product breeding, relates to a culture method of macrobrachium rosenbergii, and particularly relates to a culture method of macrobrachium rosenbergii seedlings.
Background
In recent years, with the progress of society and the continuous improvement of living standard of people, the demand of the market for aquatic products is more and more large, so that the aquatic product breeding industry develops rapidly. The aquatic product breeding industry which is rapidly developed by benefiting from modern breeding technology lays a solid foundation for ensuring the vegetable basket demand of people. However, the stimulation of environmental, disease and nutritional factors in the existing breeding mode often causes the rapid reduction of the transparency and the smoothness of the water body, endangers the growth and development of aquatic products, and becomes a prominent contradiction to be urgently solved for the sustainable development of the aquatic product breeding industry in China.
The aquatic product refers to animal and its processed product produced in marine and fresh water fishery. Fresh and live aquatic products are classified into four categories, namely fish, shrimp, crab and shellfish. Aquaculture is the production activity of breeding, cultivating and harvesting aquatic animals and plants under human control, and aquaculturists usually sell purchased aquatic seedlings by raising them to a certain size. When the aquatic products are cultured, probiotics, vitamins and the like are needed to improve the resistance of the aquatic products and inhibit the reproduction of harmful microorganisms in a pond.
The macrobrachium rosenbergii is an important aquatic product, has high growth speed, wide food recipe and rich nutrition, has thin shell and fat body, fresh and tender meat quality and delicious taste, is rich in the flavor of common freshwater shrimps, is filled with gonads in the head and the chest of the mature macrobrachium rosenbergii, has special delicious taste similar to crab spawn, has great market demand at home and abroad, is in short supply, and has important economic value. The common culture method of the macrobrachium rosenbergii seedlings in the prior art does not solve the living characteristics of mutual killing of the macrobrachium rosenbergii, and the phenomena of poor constitution, slow growth, easy morbidity and the like are easy to occur. The macrobrachium rosenbergii seedlings cultured by the common culture method have low growth speed, small weight, long time for reaching the market standard and low yield, so that the economic efficiency is continuously reduced.
Therefore, a more reasonable culture method of the macrobrachium rosenbergii seedlings is found, which meets the market demand and has higher market value and social value.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a culture method of the seedling of the Macrobrachium rosenbergii, which can obviously improve the adaptability of the larva of the Macrobrachium rosenbergii to fresh water and improve the survival rate of the Macrobrachium rosenbergii by synchronously adjusting the salinity, the temperature and the bait in the culture process of the larva of the Macrobrachium rosenbergii, so that the Macrobrachium rosenbergii grows faster, and has fast food intake and good stability.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a culture method of macrobrachium rosenbergii seedlings comprises the following steps:
step S1, preparing a seedling culture pond: selecting a cement pond or a soil pond capable of controlling temperature, ensuring that the bottom of the pond is smooth and has no sludge, the wall of the pond is firm and has no water leakage, and irrigation and drainage facilities are convenient; then spraying disinfectant to the whole pool for disinfection, and washing the whole pool clean by clear water; adding water into a culture pond, detecting the amount of potassium ions and calcium ions in water, regulating with potassium chloride and calcium chloride to ensure that the concentration of the potassium ions and the calcium ions in the water is 160mg/L, testing water with the Macrobrachium rosenbergii seedlings, adding some aquatic plants into the culture pond if the Macrobrachium rosenbergii seedlings do not die, and adding the water to a certain water depth;
step S2, stocking seedlings: transferring the larvae of Macrobrachium rosenbergii caught by a filter screen into the larvae, controlling stocking density, water temperature and dissolved oxygen, controlling the water temperature to 22 ℃, increasing the water temperature by 0.5-1 ℃ every day, heating to 2 ℃, stopping the breeding for 1 day, and heating to 1 ℃ every day until the water temperature reaches 27-30 ℃;
step S3, feeding bait: feeding animal baits to the young seedlings 3-5 days before the culture is started, wherein the feeding amount of the animal baits is 15-20% of the weight of the shrimps every day, and feeding for 3 times every day; feeding artificial compound feed later, wherein the daily feeding amount is calculated according to 15% -20% of the weight of the juvenile shrimps, and feeding for 3-5 times a day, wherein the feeding amount in the evening is half of the daily feeding amount;
step S4, daily management: because the density of the stocking seedlings is high, continuous aeration for 24 hours is generally required for oxygenation, if the floating head phenomenon occurs, new water or oxygen supply agent is injected immediately to increase the dissolved oxygen in the pond water; applying 1 time of antibiotics 6-8 days after stocking the seedlings, carrying out whole-pool splashing sterilization, and then using 1 time every 10-15 days to prevent diseases; observing the activity condition of the shrimp groups, and paying attention to whether harmful organisms invade, if so, eliminating the harmful organisms in time; recording every day, measuring the water temperature at regular time, and measuring the PH value, dissolved oxygen and salinity at regular time; the growth condition of the seedlings is checked and determined every 3 days, so that the culture management is established on a certain scientific basis, and the high survival rate of the seedling culture is ensured.
Further, the disinfectant comprises the following components in parts by weight: 5-10 parts of sodium dichloroisocyanurate, 3-5 parts of potassium permanganate, 2-5 parts of sodium hypochlorite, 1-3 parts of chlorine dioxide, 3-5 parts of glycine-based chitosan quaternary ammonium salt and 300 parts of water 200-.
Preferably, the preparation method of the glycine-based chitosan quaternary ammonium salt comprises the following steps: adding chloroacetyl glycyl glycine and water-soluble chitosan into an organic solvent, stirring and reacting for 6-8 hours at the temperature of 60-80 ℃, and then removing the solvent by rotary evaporation to obtain the glycine-based chitosan quaternary ammonium salt.
Preferably, the mass ratio of the chloracetyl glycyl glycine to the water-soluble chitosan to the organic solvent is 1 (3-5) to 15-20.
Preferably, the organic solvent is selected from at least one of N, N-dimethylformamide, dimethyl sulfoxide and pyridine.
Preferably, the aquatic plant is at least one of duckweed, hornworts, mullets, water hyacinth and water peanuts.
Preferably, the certain water depth is 0.5-0.7 m.
Preferably, the stocking density is 2000-4000 tails per square meter.
Preferably, the dissolved oxygen amount is 5.5-5.8 mg/L.
Preferably, the animal bait is one or a combination of several of artemia nauplii, earthworms, rotifers and fly maggots in any proportion.
Preferably, the artificial compound feed is prepared by mixing the following raw materials in parts by weight: 4-8 parts of egg-steaming paste, 2-5 parts of cooked fish meat particles, 3-5 parts of soybean curb residues, 0.5-1 part of shrimp slices, 1-3 parts of a vinasse, 1-3 parts of a grape worm, 2-4 parts of a maggot bowing, 0.1-0.4 part of immune polysaccharide, 0.1-0.3 part of algal polysaccharide, 0.5-1 part of earthworm and 0.2-0.5 part of immune enhancing function Chinese herbal medicine extract.
Preferably, the immune polysaccharide is selected from one or a mixture of more than one of beta-glucan, mannan oligosaccharide, fructo oligosaccharide and isomalto oligosaccharide.
Preferably, the Chinese herbal medicine extract with the immune enhancing function is prepared by extracting the following Chinese herbal medicines in parts by weight: 2-5 parts of astragalus root, 1-3 parts of licorice, 1-4 parts of cordyceps sinensis and 1-3 parts of seaweed.
Preferably, the antibiotic is at least one of terramycin and chloramphenicol, and the dosage is 0.4-0.8g per cubic meter of water.
Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages: the culture method of the macrobrachium rosenbergii larvae can obviously improve the adaptability of the larvae to fresh water and improve the survival rate of the macrobrachium rosenbergii by synchronously adjusting the salinity, the temperature and the bait in the culture process of the larvae, so that the growth speed of the larvae is accelerated, the feeding speed is high, the stability is good, the nutrition is rich, the immunity is enhanced, and the method is convenient and easy to implement and has low cost.
Detailed Description
The invention relates to a culture method of macrobrachium rosenbergii seedlings, which comprises the following steps:
step S1, preparing a seedling culture pond: selecting a cement pond or a soil pond capable of controlling temperature, ensuring that the bottom of the pond is smooth and has no sludge, the wall of the pond is firm and has no water leakage, and irrigation and drainage facilities are convenient; then spraying disinfectant to the whole pool for disinfection, and washing the whole pool clean by clear water; adding water into a culture pond, detecting the amount of potassium ions and calcium ions in water, regulating with potassium chloride and calcium chloride to ensure that the concentration of the potassium ions and the calcium ions in the water is 160mg/L, testing water with the Macrobrachium rosenbergii seedlings, adding some aquatic plants into the culture pond if the Macrobrachium rosenbergii seedlings do not die, and adding the water to a certain water depth;
step S2, stocking seedlings: transferring the larvae of Macrobrachium rosenbergii caught by a filter screen into the larvae, controlling stocking density, water temperature and dissolved oxygen, controlling the water temperature to 22 ℃, increasing the water temperature by 0.5-1 ℃ every day, heating to 2 ℃, stopping the breeding for 1 day, and heating to 1 ℃ every day until the water temperature reaches 27-30 ℃;
step S3, feeding bait: feeding animal baits to the young seedlings 3-5 days before the culture is started, wherein the feeding amount of the animal baits is 15-20% of the weight of the shrimps every day, and feeding for 3 times every day; feeding artificial compound feed later, wherein the daily feeding amount is calculated according to 15% -20% of the weight of the juvenile shrimps, and feeding for 3-5 times a day, wherein the feeding amount in the evening is half of the daily feeding amount;
step S4, daily management: because the density of the stocking seedlings is high, continuous aeration for 24 hours is generally required for oxygenation, if the floating head phenomenon occurs, new water or oxygen supply agent is injected immediately to increase the dissolved oxygen in the pond water; applying 1 time of antibiotics 6-8 days after stocking the seedlings, carrying out whole-pool splashing sterilization, and then using 1 time every 10-15 days to prevent diseases; observing the activity condition of the shrimp groups, and paying attention to whether harmful organisms invade, if so, eliminating the harmful organisms in time; recording every day, measuring the water temperature at regular time, and measuring the PH value, dissolved oxygen and salinity at regular time; the growth condition of the seedlings is checked and determined every 3 days, so that the culture management is established on a certain scientific basis, and the high survival rate of the seedling culture is ensured.
The disinfectant comprises the following components in parts by weight: 5-10 parts of sodium dichloroisocyanurate, 3-5 parts of potassium permanganate, 2-5 parts of sodium hypochlorite, 1-3 parts of chlorine dioxide, 3-5 parts of glycine-based chitosan quaternary ammonium salt and 300 parts of water 200-. The preparation method of the glycine-based chitosan quaternary ammonium salt comprises the following steps: adding chloroacetyl glycyl glycine and water-soluble chitosan into an organic solvent, stirring and reacting for 6-8 hours at the temperature of 60-80 ℃, and then removing the solvent by rotary evaporation to obtain the glycine-based chitosan quaternary ammonium salt. The mass ratio of the chloracetyl glycyl glycine to the water-soluble chitosan to the organic solvent is (3-5) to (15-20). The organic solvent is at least one selected from N, N-dimethylformamide, dimethyl sulfoxide and pyridine.
The aquatic plant is at least one of Spirodela polyrhiza, Goldfish algae, Carassia Maultflora champ, herba Eichhorniae, and semen Arachidis Hypogaeae. The certain water depth is 0.5-0.7 m. The stocking density is 2000-4000 tails per square meter. The dissolved oxygen amount is 5.5-5.8 mg/L. The animal bait is one or a combination of more of artemia nauplii, earthworms, rotifers and fly maggots in any proportion. The artificial compound feed is prepared by mixing the following raw materials in parts by weight: 4-8 parts of egg-steaming paste, 2-5 parts of cooked fish meat particles, 3-5 parts of soybean curb residues, 0.5-1 part of shrimp slices, 1-3 parts of a vinasse, 1-3 parts of a grape worm, 2-4 parts of a maggot bowing, 0.1-0.4 part of immune polysaccharide, 0.1-0.3 part of algal polysaccharide, 0.5-1 part of earthworm and 0.2-0.5 part of immune enhancing function Chinese herbal medicine extract. The immune polysaccharide is selected from one or a mixture of more than one of beta-glucan, mannan oligosaccharide, fructo-oligosaccharide and isomaltooligosaccharide. The Chinese herbal medicine extract with the immune enhancement function is prepared by extracting the following Chinese herbal medicines in parts by weight: 2-5 parts of astragalus root, 1-3 parts of licorice, 1-4 parts of cordyceps sinensis and 1-3 parts of seaweed. The antibiotic is at least one of oxytetracycline and chloramphenicol, and the dosage of the antibiotic is 0.4-0.8g per cubic meter of water. The culture method of the macrobrachium rosenbergii larvae can obviously improve the adaptability of the larvae to fresh water and improve the survival rate of the macrobrachium rosenbergii by synchronously adjusting the salinity, the temperature and the bait in the culture process of the larvae, so that the growth speed of the larvae is accelerated, the feeding speed is high, the stability is good, the nutrition is rich, the immunity is enhanced, and the method is convenient and easy to implement and has low cost.
The following detailed description of preferred embodiments of the invention will be made.
Example 1
Embodiment 1 provides a culture method of macrobrachium rosenbergii seedlings, comprising the following steps:
step S1, preparing a seedling culture pond: selecting a cement pond or a soil pond capable of controlling temperature, ensuring that the bottom of the pond is smooth and has no sludge, the wall of the pond is firm and has no water leakage, and irrigation and drainage facilities are convenient; then spraying disinfectant to the whole pool for disinfection, and washing the whole pool clean by clear water; adding water into a culture pond, detecting the amount of potassium ions and calcium ions in water, regulating with potassium chloride and calcium chloride to make the concentration of potassium ions and calcium ions in the water be 140mg/L, testing water with the seedlings of the Macrobrachium rosenbergii, adding some aquatic plants into the culture pond if the seedlings of the Macrobrachium rosenbergii do not die, and adding water to a certain water depth;
step S2, stocking seedlings: transferring the larvae of the Macrobrachium rosenbergii caught by a filter screen into the larvae, controlling the stocking density, water temperature and dissolved oxygen, controlling the water temperature to be 22 ℃, increasing the water temperature by 0.5 ℃ every day, heating to 2 ℃, stopping the rest for 1 day, and heating to 1 ℃ every day until the water temperature reaches 27 ℃;
step S3, feeding bait: feeding animal baits to the seedlings 3 days before the culture starts, wherein the feeding amount of the animal baits is 15 percent of the weight of the shrimps every day, and the feeding is carried out for 3 times every day; feeding artificial compound feed later, wherein the daily feeding amount is calculated according to 15% of the weight of the juvenile shrimps, and feeding for 3 times a day, wherein the daily feeding amount is half of the daily feeding amount;
step S4, daily management: because the density of the stocking seedlings is high, continuous aeration for 24 hours is generally required for oxygenation, if the floating head phenomenon occurs, new water or oxygen supply agent is injected immediately to increase the dissolved oxygen in the pond water; applying 1 time of antibiotics 6 days after the seedlings are stocked, carrying out whole pool splashing sterilization, and then using 1 time every 10 days to prevent diseases; observing the activity condition of the shrimp groups, and paying attention to whether harmful organisms invade, if so, eliminating the harmful organisms in time; recording every day, measuring the water temperature at regular time, and measuring the PH value, dissolved oxygen and salinity at regular time; the growth condition of the seedlings is checked and determined every 3 days, so that the culture management is established on a certain scientific basis, and the high survival rate of the seedling culture is ensured.
The disinfectant comprises the following components in parts by weight: 5 parts of sodium dichloroisocyanurate, 3 parts of potassium permanganate, 2 parts of sodium hypochlorite, 1 part of chlorine dioxide, 3 parts of glycine-based chitosan quaternary ammonium salt and 200 parts of water.
The preparation method of the glycine-based chitosan quaternary ammonium salt comprises the following steps: adding 1kg of chloracetyl glycyl glycine and 3kg of water-soluble chitosan into 15kg of N, N-dimethylformamide, stirring and reacting for 6 hours at the temperature of 60 ℃, and then carrying out rotary evaporation to remove the solvent to obtain the glycine-based chitosan quaternary ammonium salt.
The aquatic plants are formed by mixing duckweed, hornworts and mullets according to a mass ratio of 1:2: 5; the certain water depth is 0.5 m; the stocking density is 2000 per square meter; the dissolved oxygen amount is 5.5 mg/L.
The animal bait is prepared by mixing artemia nauplii and earthworms according to the mass ratio of 2: 4.
The artificial compound feed is prepared by mixing the following raw materials in parts by weight: 4 parts of egg paste, 2 parts of cooked fish meat particles, 3 parts of bean curd residue, 0.5 part of shrimp slices, 1 part of a wine tank, 1 part of a grape worm, 2 parts of a maggot bowing, 0.1 part of beta-glucan, 0.1 part of algal polysaccharide, 0.5 part of earthworm and 0.2 part of Chinese herbal medicine extract with immunity enhancing function.
The Chinese herbal medicine extract with the immune enhancement function is prepared by extracting the following Chinese herbal medicines in parts by weight: 2 parts of astragalus root, 1 part of licorice, 1 part of cordyceps sinensis and 1 part of seaweed.
The antibiotic is oxytetracycline, and the dosage of each time is 0.4g per cubic meter of water body.
Example 2
Example 2 provides a method for culturing young macrobrachium rosenbergii, which is substantially identical to that of example 1 except that,
the disinfectant comprises the following components in parts by weight: 7 parts of sodium dichloroisocyanurate, 3.5 parts of potassium permanganate, 3 parts of sodium hypochlorite, 1.5 parts of chlorine dioxide, 3.5 parts of glycine-based chitosan quaternary ammonium salt and 220 parts of water; the aquatic plant is formed by mixing water hyacinth and water peanuts according to the mass ratio of 3: 5; the certain water depth is 0.6 m; the stocking density is 2500 tails per square meter; the dissolved oxygen amount is 5.6 mg/L; the animal bait is formed by mixing artemia nauplii and rotifers according to the mass ratio of 2: 3.
The artificial compound feed is prepared by mixing the following raw materials in parts by weight: 5 parts of egg-steaming paste, 3 parts of cooked fish meat particles, 3.5 parts of bean curd residue, 0.6 part of shrimp slices, 1.5 parts of a wine tank, 1.5 parts of a grape worm, 2.5 parts of a maggot, 0.2 part of mannan oligosaccharide, 0.15 part of algal polysaccharide, 0.6 part of earthworm and 0.3 part of immune enhancing function Chinese herbal medicine extract.
The Chinese herbal medicine extract with the immune enhancement function is prepared by extracting the following Chinese herbal medicines in parts by weight: 3 parts of astragalus, 1.5 parts of liquorice, 2 parts of cordyceps sinensis and 1.5 parts of seaweed.
The antibiotic is chloramphenicol, and the dosage of each antibiotic is 0.5g per cubic meter of water.
Example 3
Example 3 provides a method for culturing young macrobrachium rosenbergii, which is substantially identical to that of example 1, except that,
the disinfectant comprises the following components in parts by weight: 7.5 parts of sodium dichloroisocyanurate, 4 parts of potassium permanganate, 3.5 parts of sodium hypochlorite, 2 parts of chlorine dioxide, 4 parts of glycine-based chitosan quaternary ammonium salt and 250 parts of water; the aquatic plants are formed by mixing duckweed, hornworts, multocida and water hyacinth according to the mass ratio of 1:1:2: 4; the certain water depth is 0.6 m; the stocking density is 3000 tails per square meter; the dissolved oxygen amount is 5.7 mg/L; the animal bait is prepared by mixing artemia nauplii, earthworms, rotifers and fly maggots according to the mass ratio of 1:2:3: 2.
The artificial compound feed is prepared by mixing the following raw materials in parts by weight: 6 parts of steamed egg paste, 3.5 parts of cooked fish meat particles, 4 parts of soybean curb residues, 0.8 part of shrimp slices, 2 parts of a wine tank, 2 parts of grape worms, 3 parts of a maggot bowing, 0.25 part of immune polysaccharide, 0.2 part of algal polysaccharide, 0.8 part of earthworm and 0.35 part of immune enhancing function Chinese herbal medicine extract; the immune polysaccharide is formed by mixing beta-glucan, mannan oligosaccharide, fructooligosaccharide and isomaltooligosaccharide in a mass ratio of 1:1:2: 4. The Chinese herbal medicine extract with the immune enhancement function is prepared by extracting the following Chinese herbal medicines in parts by weight: 4 parts of astragalus root, 2 parts of licorice, 3 parts of cordyceps sinensis and 2 parts of seaweed.
The antibiotic is prepared by mixing oxytetracycline and chloramphenicol at a mass ratio of 3:5, and the dosage of the antibiotic is 0.6g per cubic meter of water.
Example 4
Example 4 provides a method for culturing young macrobrachium rosenbergii, which is substantially identical to that of example 1 except that,
the disinfectant comprises the following components in parts by weight: 9 parts of sodium dichloroisocyanurate, 4.5 parts of potassium permanganate, 4 parts of sodium hypochlorite, 2.5 parts of chlorine dioxide, 4.5 parts of glycine-based chitosan quaternary ammonium salt and 280 parts of water. The aquatic plant is water hyacinth; the certain water depth is 0.65 m; the stocking density is 3500 tails per square meter; the dissolved oxygen amount is 5.7 mg/L; the animal bait is artemia salina without maggots.
The artificial compound feed is prepared by mixing the following raw materials in parts by weight: 7 parts of egg paste, 4.5 parts of cooked fish meat particles, 4.5 parts of soybean curb residues, 0.9 part of shrimp slices, 2.5 parts of a wine tank, 2.5 parts of a grape worm, 3.5 parts of a maggot, 0.35 part of isomaltooligosaccharide, 0.3 part of algal polysaccharide, 0.9 part of earthworm and 0.5 part of immune enhancing function Chinese herbal medicine extract; the Chinese herbal medicine extract with the immune enhancement function is prepared by extracting the following Chinese herbal medicines in parts by weight: 4 parts of astragalus root, 2.5 parts of licorice, 3.5 parts of cordyceps sinensis and 3 parts of seaweed.
The antibiotic is oxytetracycline, and the dosage of the antibiotic is 0.7g per cubic meter of water body.
Example 5
Example 5 provides a method for culturing young macrobrachium rosenbergii, which is substantially identical to that of example 1, except that,
the disinfectant comprises the following components in parts by weight: 10 parts of sodium metachlor, 5 parts of potassium permanganate, 5 parts of sodium hypochlorite, 3 parts of chlorine dioxide, 5 parts of glycine-based chitosan quaternary ammonium salt and 300 parts of water; the aquatic plant is water peanut; the certain water depth is 0.7 m; the stocking density is 4000 tails per square meter; the dissolved oxygen amount is 5.8 mg/L; the animal bait is artemia nauplii.
The artificial compound feed is prepared by mixing the following raw materials in parts by weight: 8 parts of steamed egg paste, 5 parts of cooked fish meat particles, 5 parts of bean curd residue, 1 part of shrimp slices, 3 parts of a wine tank, 3 parts of grape worm, 4 parts of a maggot bowing, 0.4 part of immune polysaccharide, 0.3 part of algal polysaccharide, 1 part of earthworm and 0.5 part of immune enhancement function Chinese herbal medicine extract; the immune polysaccharide is mannan oligosaccharide; the Chinese herbal medicine extract with the immune enhancement function is prepared by extracting the following Chinese herbal medicines in parts by weight: 5 parts of astragalus, 3 parts of liquorice, 4 parts of cordyceps sinensis and 3 parts of seaweed.
The antibiotic is chloramphenicol, and the dosage of each antibiotic is 0.8g per cubic meter of water.
Comparative example 1
Comparative example 1 provides a method for culturing young macrobrachium rosenbergii, which is substantially identical to that of example 1 except that no glycine-based chitosan quaternary ammonium salt is added to the disinfectant formula.
Comparative example 2
Comparative example 2 provides a method for culturing young macrobrachium rosenbergii, which is substantially identical to that of example 1 except that no immunopotentiating herbal extract is added to the artificial composite feed formulation.
Comparative example 3
Comparative example 3 provides a method for culturing young macrobrachium rosenbergii, which is substantially identical to that of example 1 except that algal polysaccharide is not added to the artificial composite feed formulation.
Comparative example 4
Comparative example 4 provides a method for culturing young macrobrachium rosenbergii, which is substantially identical to that of example 1 except that the water temperature is always controlled at 27 ℃.
Comparative example 5
Comparative example 5 provides a culture method of Macrobrachium rosenbergii seedlings, which is implemented according to example 1 of the Chinese invention patent CN 108782385A.
To further illustrate the technical effects of the culture method of the young macrobrachium rosenbergii according to the embodiment of the present invention, the survival rate of the young seedlings cultured by the culture method of the young macrobrachium rosenbergii in each of the above examples was measured as (total number of young shrimps-number of dead young shrimps)/total number of young shrimps × 100%, and the results of the tests of examples 1 to 5 and comparative examples 1 to 5 were 97.5%, 97.8%, 98.3%, 98.7%, 99.2%, 93.5%, 94.0%, 94.5%, 96.8% and 94.5%, respectively. Therefore, the survival rate of the cultured seedlings is higher by the culture method of the macrobrachium rosenbergii seedlings provided by the embodiment of the invention.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (1)
1. A culture method of macrobrachium rosenbergii seedlings is characterized by comprising the following steps:
step S1, preparing a seedling culture pond: selecting a cement pond or a soil pond capable of controlling temperature, ensuring that the bottom of the pond is smooth and has no sludge, the wall of the pond is firm and has no water leakage, and irrigation and drainage facilities are convenient; then spraying disinfectant to the whole pool for disinfection, and washing the whole pool clean by clear water; adding water into a culture pond, detecting the amount of potassium ions and calcium ions in water, regulating with potassium chloride and calcium chloride to make the concentration of potassium ions and calcium ions in the water be 140mg/L, testing water with the seedlings of the Macrobrachium rosenbergii, adding some aquatic plants into the culture pond if the seedlings of the Macrobrachium rosenbergii do not die, and adding water to a certain water depth;
step S2, stocking seedlings: transferring the larvae of the Macrobrachium rosenbergii caught by a filter screen into the larvae, controlling the stocking density, water temperature and dissolved oxygen, controlling the water temperature to be 22 ℃, increasing the water temperature by 0.5 ℃ every day, heating to 2 ℃, stopping the rest for 1 day, and heating to 1 ℃ every day until the water temperature reaches 27 ℃;
step S3, feeding bait: feeding animal baits to the seedlings 3 days before the culture starts, wherein the feeding amount of the animal baits is 15 percent of the weight of the shrimps every day, and the feeding is carried out for 3 times every day; feeding artificial compound feed later, wherein the daily feeding amount is calculated according to 15% of the weight of the juvenile shrimps, and feeding for 3 times a day, wherein the daily feeding amount is half of the daily feeding amount;
step S4, daily management: because the density of the stocking seedlings is high, continuous aeration for 24 hours is generally required for oxygenation, if the floating head phenomenon occurs, new water or oxygen supply agent is injected immediately to increase the dissolved oxygen in the pond water; applying 1 time of antibiotics 6 days after the seedlings are stocked, carrying out whole pool splashing sterilization, and then using 1 time every 10 days to prevent diseases; observing the activity condition of the shrimp groups, and paying attention to whether harmful organisms invade, if so, eliminating the harmful organisms in time; recording every day, measuring the water temperature at regular time, and measuring the PH value, dissolved oxygen and salinity at regular time; the growth condition of the seedlings is checked and determined every 3 days, so that the culture management is established on a certain scientific basis, and the high survival rate of the seedling culture is ensured;
the disinfectant comprises the following components in parts by weight: 10 parts of sodium metachlor, 5 parts of potassium permanganate, 5 parts of sodium hypochlorite, 3 parts of chlorine dioxide, 5 parts of glycine-based chitosan quaternary ammonium salt and 300 parts of water;
the aquatic plant is water peanut; the certain water depth is 0.7 m; the stocking density is 4000 tails per square meter; the dissolved oxygen amount is 5.8 mg/L; the animal bait is artemia nauplii;
the artificial compound feed is prepared by mixing the following raw materials in parts by weight: 8 parts of steamed egg paste, 5 parts of cooked fish meat particles, 5 parts of bean curd residue, 1 part of shrimp slices, 3 parts of a wine tank, 3 parts of grape worm, 4 parts of a maggot bowing, 0.4 part of immune polysaccharide, 0.3 part of algal polysaccharide, 1 part of earthworm and 0.5 part of immune enhancement function Chinese herbal medicine extract; the immune polysaccharide is mannan oligosaccharide; the Chinese herbal medicine extract with the immune enhancement function is prepared by extracting the following Chinese herbal medicines in parts by weight: 5 parts of astragalus, 3 parts of liquorice, 4 parts of cordyceps sinensis and 3 parts of seaweed;
the antibiotic is chloramphenicol, and the dosage of each antibiotic is 0.8g per cubic meter of water;
the preparation method of the glycine-based chitosan quaternary ammonium salt comprises the following steps: adding 1kg of chloracetyl glycyl glycine and 3kg of water-soluble chitosan into 15kg of N, N-dimethylformamide, stirring and reacting for 6 hours at the temperature of 60 ℃, and then performing rotary evaporation to remove the solvent to obtain glycine-based chitosan quaternary ammonium salt;
the aquatic plants are formed by mixing duckweed, hornworts and mullets according to a mass ratio of 1:2: 5; the certain water depth is 0.5 m; the stocking density is 2000 per square meter; the dissolved oxygen amount is 5.5 mg/L;
the animal bait is prepared by mixing artemia nauplii and earthworms according to the mass ratio of 2: 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910824693.9A CN110419477B (en) | 2019-09-02 | 2019-09-02 | Culture method of macrobrachium rosenbergii seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910824693.9A CN110419477B (en) | 2019-09-02 | 2019-09-02 | Culture method of macrobrachium rosenbergii seedlings |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110419477A CN110419477A (en) | 2019-11-08 |
| CN110419477B true CN110419477B (en) | 2021-09-17 |
Family
ID=68418540
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910824693.9A Active CN110419477B (en) | 2019-09-02 | 2019-09-02 | Culture method of macrobrachium rosenbergii seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110419477B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104938378A (en) * | 2015-06-03 | 2015-09-30 | 广西壮族自治区水产科学研究院 | Ecological young shrimp culture method for macrobrachium rosenbergii |
| CN105104259A (en) * | 2015-08-19 | 2015-12-02 | 莆田市生物工程研究所 | Shallow sea raft-type healthy and efficient breeding method for Panulirus stimpsoni |
| CN106071411A (en) * | 2016-07-07 | 2016-11-09 | 山东新希望六和集团有限公司 | A kind of mixed feed improving shrimp immunity vigor and preparation method thereof |
| JP6056949B1 (en) * | 2014-11-27 | 2017-01-11 | 学校法人加計学園 | Seed and seedling culture water for crustaceans such as shrimp, crab and giant clam, and seedling culture method using this |
| CN106614182A (en) * | 2016-11-25 | 2017-05-10 | 全椒县花溪湖特种水产专业合作社 | Culture method for freshwater shrimp and soft-shelled turtle polyculture |
| CN106689001A (en) * | 2016-12-08 | 2017-05-24 | 青岛理工大学 | Self-circulation prawn aquaculture method |
| CN107047161A (en) * | 2017-04-10 | 2017-08-18 | 益阳市大通湖金雁子水稻种植统防统治专业合作社 | A kind of comprehensive breeding method of paddy rice cray |
| KR20180134627A (en) * | 2017-06-09 | 2018-12-19 | 원광대학교산학협력단 | New marine forest forming method using attachment and growth of zygotes of sargassum species on the natural basalt gravels |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3673986A (en) * | 1970-11-12 | 1972-07-04 | Harold N Braunhut | Method and materials used for hatching brine shrimp |
| US6584935B2 (en) * | 2001-03-16 | 2003-07-01 | University Of Maryland Biotechnology Institute | Process for culturing crabs in recirculating marine aquaculture systems |
| CN100586302C (en) * | 2007-04-18 | 2010-02-03 | 上海邦成生物科技有限公司 | Fodder additive having immunologic enhancement in growing and preparation method thereof |
| CN101218900B (en) * | 2008-01-22 | 2012-02-22 | 中国水产科学研究院淡水渔业研究中心 | Seedling desalination method for fresh water aquiculture for penaeus monodon |
| CN101781034B (en) * | 2009-01-19 | 2013-03-20 | 北京泛博科技有限责任公司 | Water treatment agent and preparation method and application thereof |
| CN101720709B (en) * | 2010-01-08 | 2012-07-04 | 中国水产科学研究院南海水产研究所 | Culture method of penaeid shrimps in over-winter shed and culture pond thereof |
| CA2924337C (en) * | 2012-11-08 | 2020-07-28 | Planktonic As | Marine material derived from early developmental stages of barnacles |
| WO2016160141A1 (en) * | 2015-03-30 | 2016-10-06 | Royal Caridea Llc | Multi-phasic integrated super-intensive shrimp production system |
| CN105557601B (en) * | 2016-03-14 | 2017-12-19 | 上海市水产研究所 | A kind of Scatophagus argus (Linnaeus) survive the winter pattern foster with Macrobrachium rosenbergii set |
| CN108782385A (en) * | 2018-05-28 | 2018-11-13 | 苏州市毛氏阳澄湖水产发展有限公司 | A kind of cultural method of Macrobrachium rosenbergii seedling |
-
2019
- 2019-09-02 CN CN201910824693.9A patent/CN110419477B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6056949B1 (en) * | 2014-11-27 | 2017-01-11 | 学校法人加計学園 | Seed and seedling culture water for crustaceans such as shrimp, crab and giant clam, and seedling culture method using this |
| CN104938378A (en) * | 2015-06-03 | 2015-09-30 | 广西壮族自治区水产科学研究院 | Ecological young shrimp culture method for macrobrachium rosenbergii |
| CN105104259A (en) * | 2015-08-19 | 2015-12-02 | 莆田市生物工程研究所 | Shallow sea raft-type healthy and efficient breeding method for Panulirus stimpsoni |
| CN106071411A (en) * | 2016-07-07 | 2016-11-09 | 山东新希望六和集团有限公司 | A kind of mixed feed improving shrimp immunity vigor and preparation method thereof |
| CN106614182A (en) * | 2016-11-25 | 2017-05-10 | 全椒县花溪湖特种水产专业合作社 | Culture method for freshwater shrimp and soft-shelled turtle polyculture |
| CN106689001A (en) * | 2016-12-08 | 2017-05-24 | 青岛理工大学 | Self-circulation prawn aquaculture method |
| CN107047161A (en) * | 2017-04-10 | 2017-08-18 | 益阳市大通湖金雁子水稻种植统防统治专业合作社 | A kind of comprehensive breeding method of paddy rice cray |
| KR20180134627A (en) * | 2017-06-09 | 2018-12-19 | 원광대학교산학협력단 | New marine forest forming method using attachment and growth of zygotes of sargassum species on the natural basalt gravels |
Non-Patent Citations (2)
| Title |
|---|
| 罗氏沼虾人工育苗及养殖新技术;杨国梁;《科学养鱼》;20081130;第12-14页 * |
| 调整人工海水配方中钙镁钾离子浓度对罗氏沼虾蚤状幼体的影响研究;邓潜等;《农业与技术》;20120415;第105-106页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110419477A (en) | 2019-11-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102415346B (en) | Fresh water aquaculture method for penaeus vannamei | |
| CN103828748B (en) | Pond greenhouse Penaeus Vannmei, the method for three batches of alternate cultures of Macrobrachium rosenbergii | |
| CN106259090A (en) | A kind of ecological cultivation method of Ctenopharyngodon idellus | |
| CN104026415B (en) | A kind of Micropterus salmonoides freshwater aquiculture feed additive special and preparation method thereof | |
| CN104938378A (en) | Ecological young shrimp culture method for macrobrachium rosenbergii | |
| CN105746411B (en) | A kind of foster method of bottom of pond kind blueness cray fingerling company | |
| CN104256172A (en) | Odontobutis obscura parent fish feed and preparation method thereof | |
| CN105475193B (en) | Family's pond intercropping method of channel catfishes and mandarin fish | |
| CN103621432A (en) | Method for culturing red carps | |
| CN105393951A (en) | Carp breeding method | |
| CN106417122B (en) | A kind of breeding method improving high-quality freshwater shrimp seed yield | |
| CN106259094A (en) | A kind of method of Lobster cultivation | |
| CN111543363A (en) | Method for breeding and raising seedlings of red swamp crayfish | |
| CN104351091A (en) | Black carp breeding technology | |
| CN107079843B (en) | Breeding method of coregonus ussuriensis fries | |
| CN106665427B (en) | Pond greenhouse overwintering polyculture method for takifugu obscurus parents and scatophagus argus | |
| CN106259096A (en) | A kind of cultural method of Ctenopharyngodon idellus | |
| CN104970264A (en) | Mixed feed special for penaeus vannamei | |
| CN102939927A (en) | Method for improving fugu obscurus growing characters | |
| CN104488766A (en) | Culturing technology for middle-aged macrobrachium rosenbergii | |
| CN110100771A (en) | A kind of quick acclimation method cultivating pomfret parr | |
| CN105360033A (en) | Ecologically efficient shrimp and crab mixed culture method | |
| CN107646765A (en) | The cultural method of cray | |
| CN106804499A (en) | A kind of cultural method of river crab | |
| CN110419477B (en) | Culture method of macrobrachium rosenbergii seedlings |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |