CN114847414A - Functional traditional Chinese medicine fish bait for promoting gastrointestinal health of fishes - Google Patents

Functional traditional Chinese medicine fish bait for promoting gastrointestinal health of fishes Download PDF

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CN114847414A
CN114847414A CN202110153739.6A CN202110153739A CN114847414A CN 114847414 A CN114847414 A CN 114847414A CN 202110153739 A CN202110153739 A CN 202110153739A CN 114847414 A CN114847414 A CN 114847414A
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chinese medicine
traditional chinese
fish
fish bait
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张朔生
李秀英
陈唯韦
张晓燕
孟祥龙
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Shanxi University of Chinese Mediciine
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Abstract

The invention discloses a functional traditional Chinese medicine fish bait for promoting the gastrointestinal health of fishes. The traditional Chinese medicine fish bait comprises the following components in percentage by mass: 1 to 10 percent of traditional Chinese medicine compound extract, 35 to 45 percent of corn flour, 25 to 35 percent of wheat middling, 10 to 15 percent of fish meal, 3 to 5 percent of beer yeast, 3 to 6 percent of soybean oil, 1 to 2 percent of soybean lecithin, 1 percent of calcium carbonate, 0.45 to 0.55 percent of sodium alginate, 0.1 to 0.4 percent of choline chloride, 0.3 to 0.6 percent of mixed vitamin, 0.3 to 0.5 percent of mixed inorganic salt, 0.2 to 0.4 percent of taurine, 0.3 percent of thiobetaine and 0.3 percent of ferrous lactate; the traditional Chinese medicine compound extracting solution is prepared by extracting the following components in parts by mass: stir-frying 15 parts of immature bitter orange with bran; 19 parts of white atractylodes rhizome stir-fried with bran; 8 parts of lotus leaves. The traditional Chinese medicine fish bait provided by the invention can improve the stability and the utilization rate of the feed, can also obviously improve the ingestion capability of fish and promote the healthy growth of fish.

Description

Functional traditional Chinese medicine fish bait for promoting gastrointestinal health of fishes
Technical Field
The invention relates to a functional traditional Chinese medicine fish bait for promoting gastrointestinal health of fishes, and belongs to the technical field of feeds.
Background
China is the first aquaculture country in the world. In recent years, the marine fish breeding industry develops rapidly, and the feed demand is greatly increased. The various properties of the feed directly influence the food intake of fish, the amount of residual feed, the growth speed and the utilization efficiency of the feed, and can directly influence the economic benefit and the ecological benefit of the culture. The prior commonly used fish feed has poor stability in water and high settling speed, which not only causes the waste of feed nutrition, but also causes the serious pollution of water quality.
The enteritis of fishes is the most common fish disease, the diseases can be caused from fish seeds to adult fishes, particularly, eating fishes such as grass carps, crucian carps and the like are most susceptible to the diseases, and the enteritis is one of the more serious diseases in cultured fishes. When the fish body resistance is reduced due to deterioration of water quality, low dissolved oxygen, deterioration of feed and the like, the disease is frequently outbreaked. A common mode of treatment for this disease is the administration of garlic or allicin baits. However, no solution is available for preventing the disease at present, so that the traditional Chinese medicine bait for preventing and treating enteritis of fishes and promoting gastrointestinal health of the fishes needs to be provided.
Disclosure of Invention
The invention aims to provide a functional traditional Chinese medicine fish bait for promoting the gastrointestinal health of fishes, which has the advantages of strong stability in water, less residual bait and high resource utilization rate, and can improve the gastrointestinal health of the fishes, improve the ingestion capability and promote the healthy growth of the fishes.
The functional traditional Chinese medicine fish bait for promoting the gastrointestinal health of fishes provided by the invention comprises the following components in percentage by mass:
1 to 10 percent of traditional Chinese medicine compound extract, 35 to 45 percent of corn flour, 25 to 35 percent of wheat middling, 10 to 15 percent of fish meal, 3 to 5 percent of beer yeast, 3 to 6 percent of soybean oil, 1 to 2 percent of soybean lecithin, 1 percent of calcium carbonate, 0.45 to 0.55 percent of sodium alginate, 0.1 to 0.4 percent of choline chloride, 0.3 to 0.6 percent of mixed vitamin, 0.3 to 0.5 percent of mixed inorganic salt, 0.2 to 0.4 percent of taurine, 0.3 percent of thiobetaine and 0.3 percent of ferrous lactate;
the traditional Chinese medicine compound extracting solution is prepared by extracting the following components in parts by mass:
stir-frying 15 parts of immature bitter orange with bran; 19 parts of white atractylodes rhizome stir-fried with bran; 8 parts of lotus leaves.
The functional traditional Chinese medicine bait is prepared according to the following steps:
mixing the bran-fried immature bitter orange, the bran-fried bighead atractylodes rhizome and the lotus leaves, adding water, decocting for 3-4 times, combining decoction, and concentrating under reduced pressure to obtain the traditional Chinese medicine.
Specifically, the amount of the water is 7-9ml/g of mixed material, preferably 8ml/g of mixed material; the mixture is the mixture of the bran-fried immature bitter orange, the bran-fried bighead atractylodes rhizome and the lotus leaf.
Specifically, the time for each decoction is 1-2h, preferably 2 h.
Specifically, the number of times of the decoction is preferably 3;
the relative density of the Chinese medicinal compound extract is 1.0g/ml
Specifically, the vitamin mixture includes vitamin a acetate (vitamin a), vitamin D3, α -tocopherol acetate (vitamin E), menadione sodium bisulfite (vitamin K3), thiamine nitrate (vitamin B1), riboflavin (vitamin B2), pyridoxine hydrochloride (vitamin B6), cyanocobalamin (vitamin B12), D-biotin, folic acid, nicotinamide, calcium D-pantothenate, L-ascorbic acid (vitamin C), inositol, and ethoxyquinoline;
the mixed inorganic salt may be potassium chloride, magnesium sulfate, ferric sulfate, copper sulfate, zinc carbonate, zinc sulfate, cobalt carbonate, manganese sulfate, potassium iodate, calcium iodate, salt, zinc phosphate, calcium phosphate, and sodium selenite.
The functional traditional Chinese medicine fish bait for promoting the gastrointestinal health of fishes preferably comprises the following components in percentage by weight:
2% of traditional Chinese medicine compound extract, 40% of corn flour, 30% of wheat middling, 13.7% of fish meal, 4% of beer yeast, 5% of soybean oil, 1.5% of soybean lecithin, 1% of calcium carbonate, 0.5% of sodium alginate, 0.5% of choline chloride, 0.5% of mixed vitamin, 0.4% of mixed inorganic salt, 0.3% of taurine, 0.3% of thiobetaine and 0.3% of ferrous lactate.
The invention also provides a preparation method of the functional traditional Chinese medicine fish bait, which comprises the following steps:
weighing the components of the functional traditional Chinese medicine fish bait according to the mass percentage, uniformly mixing, and adding the traditional Chinese medicine compound extract and water under the stirring condition to obtain a material to be puffed; puffing the material to be puffed, such as puffing at 180 deg.C.
The traditional Chinese medicine fish bait provided by the invention can improve the stability and the utilization rate of the feed, can also obviously improve the ingestion capability of fish and promote the healthy growth of fish.
Drawings
FIG. 1 shows the effect of water addition on the decoction process.
FIG. 2 is a graph showing the effect of the decoction time on the decoction process.
FIG. 3 is a graph showing the effect of the number of times of decoction on the decoction process.
FIG. 4 is a graph showing the effect of the decoction time and the amount of water added on the naringin content (FIG. 4(a) is a contour diagram, and FIG. 4(b) is a three-dimensional response curve).
Fig. 5 is a graph showing the effect of the decoction time and the number of times of decoction on the naringin content (fig. 5(a) is a contour diagram, and fig. 5(b) is a three-dimensional response curve).
FIG. 6 is a graph showing the influence of the amount of water added and the number of times of decoction on the naringin content (FIG. 6(a) is a contour diagram, and FIG. 6(b) is a three-dimensional response curve).
Fig. 7 is a sign index of fish fed with the functional Chinese medicinal bait for promoting gastrointestinal health (fig. 7(a) is a body weight graph, fig. 7(b) is a fullness graph, and fig. 7(c) is a feed efficiency graph, wherein # represents p <0.05 compared with a blank group, and # represents p <0.05 compared with a positive control group).
Fig. 8 is a biochemical index of 2 gastrointestinal health promoting functional Chinese medicinal bait feed for fish (fig. 8(a) is gastrointestinal trypsin, fig. 8(b) is gastrointestinal lipase, fig. 8(c) is gastrointestinal amylase, fig. 8(d) is liver visceral index, fig. 8(e) is kidney visceral index, wherein # represents p <0.05 compared to blank group, p <0.05 and x represents p <0.01 compared to positive control group).
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Chromatographic conditions for measuring naringin content:
the content of naringin is determined by high performance liquid chromatography, using octadecylsilane chemically bonded silica as filler, and a Tnature-C18 column (250mm × 4.6mm, 5 μm) as chromatographic column, with volume flow of 1mL/min, column temperature of 30 deg.C, mobile phase of acetonitrile-0.1% phosphoric acid water (22: 78), and detection wavelength of 283 nm.
Preparation of control solutions:
precisely weighing appropriate amount of naringin reference substance, adding methanol to obtain solution with concentration of 0.1336mg/mL, performing ultrasonic treatment for 10min, and filtering with 0.22 μm microporous filter membrane to obtain reference substance solution.
Preparation of a test solution:
mixing the bran-fried immature bitter orange, the bran-fried bighead atractylodes rhizome and the lotus leaves, adding water, performing reflux extraction to obtain an extracting solution, evaporating to obtain an extract, adding methanol to a constant volume of 20mL, performing ultrasonic treatment for 10min, and filtering by using a 0.22-micrometer microporous filter membrane to obtain a test solution.
And (3) sample determination:
precisely sucking 10 μ L of each of the reference solution and the sample solution, and injecting into a liquid chromatograph for content determination. The HPLC chromatograms of the reference and test substances are shown in FIG. 1.
Example 1 study on extraction process of gastrointestinal health promoting Chinese herbal compound
The formula of the traditional Chinese medicine compound extracting solution comprises the following components:
bran-fried immature bitter orange 15 g; 19g of rhizoma atractylodis macrocephalae stir-fried with bran; lotus leaf 8 g.
Weighing the above medicinal materials, performing single factor analysis and response surface design on the compound by using naringin as an index, and preparing the anti-influenza floating fish bait.
1. Single factor experiment
1.1 Effect of the amount of Water added on the decocting Process
Taking 5 parts of 15g of bran-fried immature bitter orange, 10g of bran-fried bighead atractylodes rhizome and 8g of lotus leaf, putting the mixture into a round-bottom flask, respectively adding 4, 6, 8, 10 and 12 times of water, decocting for 2 times, each time for 1 hour, combining decoction, centrifuging, taking supernatant, evaporating to dryness, and adding methanol to a constant volume of 5 mL. The results are shown in FIG. 1. It can be seen that the naringin content is highest at 8 times of water.
1.2 Effect of decoction time on the decoction Process
Taking 5 parts of 15g of bran-fried immature bitter orange, 10g of bran-fried bighead atractylodes rhizome and 8g of lotus leaf, putting the mixture into a round-bottom flask, respectively adding 8 times of water, decocting for 2 times, decocting for 0.5h, 1h, 1.5h, 2h and 2.5h each time, combining the decoction, centrifuging, taking the supernatant, evaporating to dryness, and adding methanol to a constant volume of 5 mL. The results are shown in FIG. 2. It can be seen that naringin content is highest at 2h of decoction time.
1.3 Effect of the number of decocting times on the processing method
Taking 5 parts of 15g of bran-fried immature bitter orange, 10g of bran-fried bighead atractylodes rhizome and 8g of lotus leaf, putting the mixture into a round-bottom flask, adding 8 times of water, decocting for 1, 2, 3, 4 and 5 times respectively for 1 hour each time, combining decoction, centrifuging, taking supernate, evaporating to dryness, and adding methanol to a constant volume of 5 mL. The results are shown in FIG. 3. It can be seen that naringin content is highest when the number of times of decoction is 3.
2. Response surface test
2.1 design scheme
According to the single-factor test result, 3 factors of water addition amount (A) and decoction time (B) and decoction times (C) are used as response variables, naringin content (Y) is used as a response value to carry out an optimization experiment, and a 3-factor 3-level experiment design scheme is designed, wherein the design scheme is shown in table 1.
TABLE 1 analysis factors and level design table of response surface of extraction process of traditional Chinese medicine components for promoting gastrointestinal health
Figure BDA0002933620130000041
2.2 model building and significance test result analysis
(1) 3-factor 3-level 17 sets of tests are designed by using Design-expert.V8.0.6 software according to the center combination Design principle of Box-Behnken Design (BBD), and the test scheme and the results are shown in Table 2.
Table 2 design and results of response surface experimental protocol
Figure BDA0002933620130000042
Figure BDA0002933620130000051
The decocting process of the traditional Chinese medicine compound for promoting gastrointestinal health at different levels is analyzed by applying Design-expert.V8.0.6 software, contour diagrams and three-dimensional response surface diagrams are made, and the results are shown in fig. 4, fig. 5 and fig. 6. After regression fitting, the regression equation of each factor and the response value is as follows:
Y＝0.15-9.723×10 -3 A-4.585×10 -3 B﹢ 5.913×10 -3 C+1.608×10 -3 AB-1.553×10 -3 AC+1.523×10 -3 BC-0.024A 2 ﹣0.012B 2 ﹣ 8.691×10 -3 C 2
regression parameters of the effector surface were analyzed by ANOVA, and the simulations were subjected to analysis of variance and significance tests, with the results shown in table 3.
As can be seen from the analysis of variance, the regression model F is 6.14, and P is 0.0129 <0.05, which indicates that the regression model is significant, thereby showing that the model can predict and analyze the decoction of naringin; and the model determines the coefficient R 2 ＝0.8875，R 2 Adj 0.7430, Coefficient of Variation (CV) 7.43%, indicating a good model fit; f Mistaking 2.11, P0.2419 is more than 0.05, the degree of model mismatching is not significant, and the model is selected appropriately and can be used for the process analysis of naringin. The residual error of the model may be generated by random error, and is obtained from data in the table, wherein 1-time item A has significant influence on the model, no interactive item has significant influence, and 2-time item A 2 、B 2 Has a significant effect on the model.
TABLE 3 analysis of variance results
Figure BDA0002933620130000052
The response surface method is utilized to carry out reasonable optimization, and the optimal process for obtaining the comprehensive influence of each influence factor is as follows: adding water 7.57 times, decocting for 1.90 hr for 3.34 times, and collecting naringin at 0.150503 mg/g. Considering the convenience of practical operation, 8 times of water is added, and the decoction is carried out for 3 times, 2 hours each time.
3. Verification experiment
According to the optimized optimal process, 3 parts of naringin are prepared from the same batch of medicinal materials in parallel, the naringin content is determined by analyzing and detecting according to the optimal extraction process of the optimized gastrointestinal health promoting Chinese herbal compound, and the result is shown in table 4. The model is similar to the model predicted value (0.150503mg/ml), which shows that the established process model has better predictability, the optimized processing process is reasonable, and the method has guiding significance for actual production.
Table 4 process verification experimental results
Figure BDA0002933620130000061
Example 2 preparation of functional Chinese medicinal fish bait for promoting gastrointestinal health
1. Preparation of functional Chinese herbal compound liquid medicine for promoting gastrointestinal health
Extracting the Chinese medicinal compound liquid according to the optimal extraction process, and concentrating under reduced pressure until the relative density is 1.0 to obtain the functional Chinese medicinal effective components for promoting gastrointestinal health.
2. Dosage and adding mode of Chinese medicinal components
The dosage of the traditional Chinese medicine components is as follows: the traditional Chinese medicine active ingredient extract is added according to the mass ratio of 2 percent of the whole fish bait. Therefore, 1Kg of fish bait is prepared, and 20mL of the effective extracting solution of the traditional Chinese medicine with the concentration of 1g/mL is added.
The adding mode is as follows: adding the Chinese medicinal effective extract liquid into water in the form of adhesive.
3. Preparation and evaluation of puffed gas-producing floating fish bait for promoting gastrointestinal health
The basic fish feed formula comprises the following components: 40% of rice flour, 30% of wheat middling, 13.7% of fish meal, 4% of beer yeast, 5% of soybean oil, 1.5% of soybean lecithin, 1% of calcium carbonate, 0.5% of sodium alginate, 0.5% of choline chloride, 0.5% of vitamin mixture, 0.4% of inorganic salt mixture, 0.3% of taurine, 0.3% of thiobetaine and 0.3% of ferrous lactate.
Wherein the mixed vitamins include vitamin A acetate (vitamin A), vitamin D3, alpha-tocopherol acetate (vitamin E), menadione sodium bisulfite (vitamin K3), thiamine nitrate (vitamin B1), riboflavin (vitamin B2), pyridoxine hydrochloride (vitamin B6), cyanocobalamin (vitamin B12), D-biotin, folic acid, nicotinamide, calcium D-pantothenate, L-ascorbic acid (vitamin C), inositol, and ethoxyquinoline; the mixed inorganic salt comprises potassium chloride, magnesium sulfate, ferric sulfate, copper sulfate, zinc carbonate, zinc sulfate, cobalt carbonate, manganese sulfate, potassium iodate, calcium iodate, salt, zinc phosphate, calcium phosphate, and sodium selenite.
Weighing the components of the basic fish feed in parts by mass, uniformly mixing, adding the traditional Chinese medicine compound liquid medicine for promoting gastrointestinal health and a proper amount of water under the stirring condition, and preparing the material to be puffed. Then adding the mixture into a feed bulking machine for bulking and granulating at 180 ℃.
The floating type energy and the dissolving and dispersing performance of the fish bait for promoting gastrointestinal health are determined by referring to an appendix release degree determination method of the fourth part of the 'Chinese pharmacopoeia' of 2015 edition. Researches find that the prepared puffed bait material for promoting gastrointestinal health has a floating time of 12 hours and a dissolving time of 24 hours.
Example 3 pharmacodynamic experiment research on functional traditional Chinese medicine fish feed for promoting gastrointestinal health
1. Experimental methods
Randomly dividing 60 young fishes into a blank control group, a positive control group and an experimental group, wherein 20 fish in each group are placed in a fish tank with the temperature of more than 18 ℃. The blank control group, the positive control group and the experimental group are respectively fed with common puffed bait, garlicin bait (with garlicin content of 10%) and Chinese medicinal puffed bait for promoting gastrointestinal health in equal amount for 60 days. Recording the number of the experimental fishes, the death, the feed amount, the weight, the body length and the feeding days in the culture process of the experimental fishes, and calculating the growth performance indexes.
Survival rate is terminal fish number/initial fish number 100%;
specific growth rate [ Ln (average end body weight) -Ln (average initial body weight) ]/day of food intake 100%;
weight gain rate ═ (terminal weight-initial weight)/initial weight 100%;
body weight/body length 3 *100％；
Feed efficiency is fish body weight gain (wet weight)/total dry matter intake;
after the rearing was finished, the experimental fish was weighed. The experimental fish heart, liver pancreas, intestine, etc. were dissected and weighed, and the visceral coefficient was calculated, which is the visceral mass/body weight 100%. Trypsin, lipase and enteroamylase in the intestinal tract were detected using a biochemical analyzer.
2. Index of drug effect
2.1 indicators of physical signs
The experiment was performed under the premise of ensuring no difference in initial body weight among the groups of fish, and the results are shown in table 5 and fig. 7, in which the body weight increase of the experimental group is more significant (p <0.01) compared with the positive control group, and no significant difference is observed with the blank group. Compared with the positive control group, the experimental group has a rising trend of the fullness of the experimental fish (p <0.05) and no significant difference with the blank group, compared with the positive control group, the experimental group has a significantly increased feed efficiency (p <0.05) and no significant difference with the blank group, compared with the positive control group, and compared with the positive control group, the experimental group has a significantly increased growth rate (p <0.01) and no significant difference with the blank group.
According to the sign index results, the Chinese medicinal puffed bait for promoting gastrointestinal health can better prevent fish from suffering from enteritis, maintain normal growth of the fish and has small pollution to water quality.
2.2 Biochemical index
As shown in table 6 and fig. 8, intestinal trypsin was significantly increased (p <0.01) in the experimental group compared to the positive control group, and was not significantly different from the blank group. Compared with a positive control group, the intestinal lipase and the intestinal amylase of the experimental group are remarkably reduced (P is less than 0.05), and the intestinal lipase and the intestinal amylase of the experimental group have no remarkable difference with a blank group. Compared with a positive control group, the liver visceral coefficient of the experimental group has significant difference (P is less than 0.05), and has no significant difference with a blank group. Compared with the positive control group, the kidney internal organ index of the experimental group has an ascending trend (P is less than 0.05), and has no significant difference with the blank group.
According to the biochemical index results, the functional traditional Chinese medicine bait for promoting gastrointestinal health has a better effect of preventing enteritis of fishes and promoting healthy growth of the fishes.
In conclusion, the invention can prevent fish from enteritis, promote fish health, improve the disease resistance of fish and reduce water pollution.
TABLE 5 physical sign indexes of functional traditional Chinese medicine bait for promoting gastrointestinal health
Figure BDA0002933620130000081
Comparison with positive control group (p <0.05) # (p <0.01) # (P <0.01) #
TABLE 6 Biochemical index of functional Chinese medicinal fish bait for promoting gastrointestinal health
Figure BDA0002933620130000082
(p <0.05) # (p <0.01) compared with positive control group # (p <0.05) # # (p <0.01) compared with blank group
Example 4 evaluation of safety of functional Chinese medicinal fish feed for promoting gastrointestinal health
TABLE 7 grading Standard of acute toxicity of Fish
Fish initiation LC50(mg/L) ＜1 1-100 100-1000 1000-10000 ＞10000
Toxicity grading High toxicity High toxicity Moderate toxicity Low toxicity Micro-toxicity (non-toxicity)
40 fish tails were randomly selected, 3 experimental groups and one control group were set, and each group was repeated once except for the control group, with 10 fish in each group. The test group is administered with the compound extract of Chinese medicinal materials by intragastric administration of 0.1ml each time every day, and the control group is administered with the same amount of normal saline. The experimental fish is fed one day before the experiment, the experimental fish is not fed in the experiment process, the experimental fish is observed for 7 days in detail, and the motion state and the death condition of the experimental fish are recorded.
Through experimental observation, when the traditional Chinese medicine compound extract is fed by 50g/L, no death condition occurs in an experimental group, and the movement state of the fish is normal, which shows that the LD of the traditional Chinese medicine compound extract is 50 >10 4 mg/L, as can be seen from Table 7, the acute toxicity grade of the compound traditional Chinese medicine component is slightly toxic (nontoxic), and the toxicity of the compound traditional Chinese medicine extract with the content of 10% is tested, and has no substantial difference with the toxicity, so that the functional traditional Chinese medicine fish bait for preventing influenza, with the compound traditional Chinese medicine component content of 1-10%, is safer.

Claims (8)

1. A functional traditional Chinese medicine fish bait for promoting fish gastrointestinal health comprises the following components in percentage by mass:
1 to 10 percent of traditional Chinese medicine compound extract, 35 to 45 percent of corn flour, 25 to 35 percent of wheat middling, 10 to 15 percent of fish meal, 3 to 5 percent of beer yeast, 3 to 6 percent of soybean oil, 1 to 2 percent of soybean lecithin, 1 percent of calcium carbonate, 0.45 to 0.55 percent of sodium alginate, 0.1 to 0.4 percent of choline chloride, 0.3 to 0.6 percent of mixed vitamin, 0.3 to 0.5 percent of mixed inorganic salt, 0.2 to 0.4 percent of taurine, 0.3 percent of thiobetaine and 0.3 percent of ferrous lactate;
the traditional Chinese medicine compound extracting solution is prepared by extracting the following components in parts by mass:
stir-frying 15 parts of immature bitter orange with bran; 19 parts of white atractylodes rhizome stir-fried with bran; 8 parts of lotus leaves.
2. The functional traditional Chinese medicine fish bait as claimed in claim 1, which is characterized in that: the traditional Chinese medicine compound extracting solution is prepared according to the following steps:
mixing the bran-fried immature bitter orange, the bran-fried bighead atractylodes rhizome and the lotus leaves, adding water to decoct for 3-4 times, combining decoction, and concentrating under reduced pressure to obtain the traditional Chinese medicine.
3. The functional traditional Chinese medicine fish bait as claimed in claim 2, which is characterized in that: the amount of the water is 7-9ml/g of a mixed material, and the mixed material is a mixture of the bran-fried immature bitter orange, the bran-fried bighead atractylodes rhizome and the lotus leaves.
4. The functional traditional Chinese medicine fish bait as claimed in claim 2 or 3, which is characterized in that: the time for decocting each time is 1-2 hours.
5. The functional traditional Chinese medicine fish bait as claimed in claim 4, which is characterized in that: the amount of the water is 8ml/g of the mixed material;
the number of times of decoction is 3;
the time for decocting each time is 2 hours;
the relative density of the traditional Chinese medicine compound extracting solution is 1.0 g/ml.
6. The functional traditional Chinese medicine fish bait according to any one of claims 1 to 5, which is characterized in that: the mixed vitamins include vitamin A acetate and vitamin D 3 Alpha-tocopheryl acetate, menadione sodium bisulfite, thiamine nitrate, riboflavin, pyridoxine hydrochloride, cyanocobalamin, D-biotin, folic acid, nicotinamide, calcium D-pantothenate, L-ascorbic acid, inositol, and ethoxyquinoline;
the mixed inorganic salt comprises potassium chloride, magnesium sulfate, ferric sulfate, copper sulfate, zinc carbonate, zinc sulfate, cobalt carbonate, manganese sulfate, potassium iodate, calcium iodate, salt, zinc phosphate, calcium phosphate and sodium selenite.
7. A method for preparing the functional Chinese medicine fish bait as claimed in any one of claims 1 to 6, which comprises the following steps:
weighing the components of the functional traditional Chinese medicine fish bait according to the mass percentage, uniformly mixing, and adding the traditional Chinese medicine compound extract and water under the stirring condition to obtain a material to be puffed; and (3) puffing the material to be puffed to obtain the puffed food.
8. Use of the functional traditional Chinese medicine fish bait according to any one of claims 1 to 6 for promoting gastrointestinal health of fish.
CN202110153739.6A 2021-02-04 2021-02-04 Functional traditional Chinese medicine fish bait for promoting gastrointestinal health of fishes Pending CN114847414A (en)

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101062157A (en) * 2007-05-29 2007-10-31 南京中山制药有限公司 Process for preparing particles for Zhizhu
CN105231100A (en) * 2015-11-16 2016-01-13 江苏华威农牧发展有限公司 Feed capable of preventing liver and gallbladder syndromes of crucian carps under high-density raising conditions
CN106578657A (en) * 2016-10-20 2017-04-26 南宁海世界生物科技有限公司 Grass carp Chinese medicinal feed additive and preparation method thereof
CN109198264A (en) * 2018-09-26 2019-01-15 佛山市顺德区旺海饲料实业有限公司 A kind of grass carp expanded pellet diet and preparation method thereof reducing the high temperature season death rate
CN109907186A (en) * 2019-04-26 2019-06-21 大连海洋大学 A kind of peacock fish meal and its preparation method and application
CN110810671A (en) * 2019-11-27 2020-02-21 华南农业大学 Compound feed capable of promoting intestinal health of juvenile carp and preparation method thereof
CN111227138A (en) * 2020-03-06 2020-06-05 佛山市南海区杰大饲料有限公司 Expanded compound feed capable of replacing live fish and feeding mandarin fish in whole process and preparation method thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101062157A (en) * 2007-05-29 2007-10-31 南京中山制药有限公司 Process for preparing particles for Zhizhu
CN105231100A (en) * 2015-11-16 2016-01-13 江苏华威农牧发展有限公司 Feed capable of preventing liver and gallbladder syndromes of crucian carps under high-density raising conditions
CN106578657A (en) * 2016-10-20 2017-04-26 南宁海世界生物科技有限公司 Grass carp Chinese medicinal feed additive and preparation method thereof
CN109198264A (en) * 2018-09-26 2019-01-15 佛山市顺德区旺海饲料实业有限公司 A kind of grass carp expanded pellet diet and preparation method thereof reducing the high temperature season death rate
CN109907186A (en) * 2019-04-26 2019-06-21 大连海洋大学 A kind of peacock fish meal and its preparation method and application
CN110810671A (en) * 2019-11-27 2020-02-21 华南农业大学 Compound feed capable of promoting intestinal health of juvenile carp and preparation method thereof
CN111227138A (en) * 2020-03-06 2020-06-05 佛山市南海区杰大饲料有限公司 Expanded compound feed capable of replacing live fish and feeding mandarin fish in whole process and preparation method thereof

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