CN102696908A - Chinese medicinal food with function of enhancing immunity - Google Patents

Chinese medicinal food with function of enhancing immunity Download PDF

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CN102696908A
CN102696908A CN2012101569453A CN201210156945A CN102696908A CN 102696908 A CN102696908 A CN 102696908A CN 2012101569453 A CN2012101569453 A CN 2012101569453A CN 201210156945 A CN201210156945 A CN 201210156945A CN 102696908 A CN102696908 A CN 102696908A
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CN102696908B (en
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陈炅然
王玲
崔东安
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Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
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Lanzhou Institute of Animal Husbandry and Veterinary Medicine CAAS
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Abstract

The invention discloses a healthcare food for adult pet dogs, which is prepared by adding a certain amount of a group of Chinese medicinal compounds which comprise astragalus mongholicus, saposhnikovia divaricata, poria, atractylodes, angelica, schisandra and licorice into a common food for pets. The healthcare food for adult pet dogs has the healthcare efficacy of strengthening the body resistance to consolidate the constitution, invigorating the spleen, promoting the secretion of saliva or body fluid and tonifying the lever and the kidney and the functions of increasing the appetite of pets, improving the capacity of resisting a variety of diseases and reducing the stress response and enhancing immunity. The healthcare food can be fed to the adult pet dogs in the subclinical infection period of infectious diseases to assist to the anti-infection treatment.

Description

A kind of prescriptions of traditional Chinese medicine dog grain with immune enhancing function
Technical field:
The present invention relates to a kind of prescription grain that becomes dog, the health food that particularly a kind of pet becomes dog to use.
Background technology:
At present, animal is primarily aimed at infectious diseases with the treatment medicine.Antibiotic, antimicrobial chemical medicine, anti parasitic antibiotic, chemicals all are that pharmaceutically-active object target spot is locked in (like bacteria cell wall, bacterial ribosome etc.) on the pathogen without exception, and have ignored the utilization of the phylactic power defensive power of animal body self.Since 20th century, the physician of rare strategic perspective has not proposed clearly that " therapeutic first principle is the utilization of spontaneous recovery power." with respect to the antibiotic therapy method; the modern medical service pattern becomes at present the treatment pattern that " prevent, control, support " combines by simple treatment disease; the development trend of novel therapeutic method is non-specific enhancing innate immunity responsing reaction, to improve the immune defense ability of body.
Research in recent years confirms that nutrition, disease and immune three are reciprocal causations, complementary.Nutrition can improve body to the replying and patient's immunologic function of disease, and disease can cause malnutrition and immune deficiency, and immunity can influence body and exert an influence to the utilization of nutrient matrix with to the prognosis of disease.In order to adapt to the needs of pet clinical treatment, wait some developed countries the America and Europe, pet prescription grain has become indispensable supplemental treatment measure when the treatment some diseases.
Existing dog grain can only provide daily energy needed of dog and basic nutrition element, but to improving the dog immunologic function and reducing the not effect of pet stress reaction.
Summary of the invention
The present invention provides a kind of and overcomes the prior art deficiency, have and holding up that this is solid just, invigorating the spleen is promoted the production of body fluid, the effect of liver-kidney tonifying, can raise immunity, improve resistance against diseases, and reduce the one-tenth dog prescriptions of traditional Chinese medicine grain of adult pet dog stress reaction.
Include in the prescriptions of traditional Chinese medicine dog grain of the present invention: weight ratio is 2: 1: 1: 0.5 fresh poultry meat, fish meal, bone meal and Normal juice hepar siccatum 20-40 weight portion, weight ratio are 1: 1 soyabean protein powder and dregs of beans 10-20 weight portion, and weight ratio is 1: 1 corn flour and wheat flour 20-40 weight portion; Weight ratio is 2: 1 vegetable oil and a deep sea fish oil 1-10 weight portion; Weight ratio is 2: 1 Vegetable powder and a dry tomato 1-6 weight portion, spirulina 1-6 weight portion, betaine 0.5-1.0 weight portion; Salt 0.25-0.50 weight portion; White sugar 0.5-1.8 weight portion, brewer's yeast 1-3 weight portion, lactic acid bacteria 1-3 weight portion; Microelement pre-mix material 0.5-1.0 weight portion; The weight ratio of Mg in the microelement pre-mix material: Fe: Cu: Zn: Mn: I equals 6.52: 5.07: 0.24: 5.26: 2.69: 0.65, and vitamin premix 0.5-1.0 weight portion, VB1 in the vitamin premix: riboflavin: pantothenic acid: niacin: VK: folic acid: biotin: VB 12: the weight ratio of choline: VA: VD: VE is 1.02: 1.04: 3.75: 3.95: 0.6925: 0.128: 1.25: 0.50: 4.50: 1.25: 0.10: 10.00.Except that aforementioned component; Also have 5-15 parts by weight of Chinese traditional medicine compound in the dog grain of the present invention, Chinese medicine is wherein formed and weight ratio is: the Radix Astragali: windproof: Poria cocos: the bighead atractylodes rhizome: Radix Angelicae Sinensis: the fruit of Chinese magnoliavine: Radix Glycyrrhizae equals 80-110: 15-30: 10-30: 15-25: 30-40: 20-40: 5-15.
The present invention is applicable to that also can add weight ratio in the prescriptions of traditional Chinese medicine dog grain of adult dogs is 1: 1 beef flavor and chicken essence 0.20-0.50 weight portion.
Also can be added with 0.3% mould inhibitor in the prescriptions of traditional Chinese medicine dog grain that is applicable to adult dogs of the present invention in addition.
Visible by above content; The present invention adds a certain amount of one group of Chinese medicine compound prescription in pet grain commonly used; This pet grain is had holding up that this is solid just, invigorating the spleen is promoted the production of body fluid, the effect of liver-kidney tonifying, can increase pet appetite, improve the ability that pet is resisted multiple disease; Reduce the pet stress reaction (vaccine stress, change food stress, environmental stress etc.), the function of enhance immunity.Supplemental treatment when this prescriptions of traditional Chinese medicine grain is applicable to adult dogs infectious disease subclinical infection phase and anti-infective therapy.
Modern Chinese medicine pharmacy result of study shows that the active component that comes from Chinese medicine has the double action of nutrient and pharmacologically active concurrently.Traditional Chinese medicine has a long history and rich experience aspect health care.Based on this, the invention property animal nutrition, traditional Chinese veterinary medicine and Chinese medicine immunopharmacology are integrated and innovated, having formed with " origin conditioning " is core, follows the prescriptions of traditional Chinese medicine grain product of " food and medicine consangunity " principle.Its starting point is to pay close attention to the pet health growth.
The prescriptions of traditional Chinese medicine dog grain that the present invention relates to is theoretical according to modern Animal nutrition, on the research basis of strengthening the pet food nutrient balance; Under Chinese veterinarian's theoretical direction, carry out prescription, adjust through trophic structure pet food; Utilization has the Chinese medicine compound prescription that invigorating qi for consolidating superficies, nourishing liver and kidney, invigorating the spleen promote the production of body fluid, and makes up the prescriptions of traditional Chinese medicine grain of conditioning pet dog body autoimmunity vis medicatrix naturae, and its compatibility takes into full account the demand of palatability, Acidity of Aikalinity and the Nutrition and Metabolism of finished product; Creationary traditional Chinese medicine and pet food are integrated and improved; Being the product that traditional traditional Chinese veterinary medicine and pet clinical nutriology combine, is the best agent of " food and medicine consangunity " theory, through the adjustment of dietary structure and comprehensive opsonic action of Chinese medicine compound prescription; Action target spot is locked in host's immune organ; Start, promote immunocyte growth, differentiation, maturation, promote the generation of immune response, recover normal physiological state (rehabilitation, heilen).Supplemental treatment when being specially adapted to infectious disease subclinical infection phase and anti-infective therapy.The development of prescriptions of traditional Chinese medicine grain will be made rival claims as an equal to similar domestic brand and import brand provides best annotation.
Because having a sharp sense of smell of dog; After adding traditional Chinese medicine ingredients, may influence the appetite of dog to a certain extent; The present invention is according to the having a sharp sense of smell of dog, characteristics that taste function is more weak; Add a certain proportion of beef flavor, chicken essence through repetition test, can effectively cushion flavour of a drug, strengthened appetite of pets.
The present invention has the mould inhibitor that also can be added with 0.3%Wt in the prescription dog grain that is applicable to adult dogs of immune enhancing function in addition, makes pet grain of the present invention that long storage period can be arranged.
The specific embodiment
Below in conjunction with embodiment the present invention is described further.
(1) at first prepare basic nutritious diet, its basic composition adopts following weight proportioning (in the 1000g basal diet):
(weight ratio of fresh poultry meat, fish meal, bone meal and Normal juice hepar siccatum is 2: 1: 1: 0.5) for fresh poultry meat, fish meal, bone meal and Normal juice hepar siccatum 20-40 weight portion; Soyabean protein powder and dregs of beans 10-20 weight portion (weight ratio of soyabean protein powder and dregs of beans is 1: 1); Corn flour and wheat flour 20-40 weight portion (weight ratio of corn flour and wheat flour is 1: 1); (weight ratio of vegetable oil and deep sea fish oil is 2: 1, Vegetable powder and dry tomato 1-6 weight portion (weight ratio of Vegetable powder and dry tomato is 2: 1), spirulina 1-6 weight portion for vegetable oil and deep sea fish oil 1-10 weight portion; Betaine 0.5-1.0 weight portion; Salt 0.25-0.50 weight portion, beef flavor, chicken essence 0.20-0.50 weight portion (weight ratio of beef flavor and chicken essence 1: 1), sucrose 0.5-1.8 weight portion; Brewer's yeast 1-3 weight portion; Lactic acid bacteria 1-3 weight portion, microelement pre-mix material 0.5-1.0 weight portion (weight ratio of Mg in the microelement pre-mix material: Fe: Cu: Zn: Mn: I equals 6.52: 5.07: 0.24: 5.26: 2.69: 0.65), vitamin premix 0.5-1.0 weight portion (VB1: riboflavin: pantothenic acid: niacin: VK: folic acid: biotin: VB 12: the weight ratio of choline: VA: VD: VE is 1.02: 1.04: 3.75: 3.95: 0.6925: 0.128: 1.25: 0.50: 4.50: 1.25: 0.10: 10.00).Each component is mixed, and it is for use to stir.
(2) preparation 5-15 weight portion Chinese medicine compound prescription, its basic composition and weight proportion are following:
Chinese medicine is wherein formed and weight proportion is: Radix Astragali 80-110 weight portion, windproof 15-30 weight portion, Poria cocos 10-30 weight portion, bighead atractylodes rhizome 15-25 weight portion, Radix Angelicae Sinensis 30-40 weight portion, fruit of Chinese magnoliavine 20-40 weight portion, Radix Glycyrrhizae 5-15 weight portion.
With above each component ultramicro grinding, 200 orders sieve, and mix that to process powder for use.
(3) in basic nutritious diet, add Chinese medicine compound prescription powder, add 0.3% mould inhibitor elsewhere, after mixing was stirred, granulation was carried out expanded, processes expanded pellet feed.
Zoopery
Test a safety evaluatio and subchronic toxicity test
1. animal divides into groups and feed
The Wistar rat, body weight 200~240g, male and female half and half are available from Lanzhou Institute of Biological Products.40 rats are divided into 4 groups at random, and 10 every group, male and female half and half.The 1st group is control group, the basic nutritious diet of feeding every day; 2nd, 3,4 groups is processed group, adds prescriptions of traditional Chinese medicine grain in 25%, 50%, 100% ratio successively, and every day 1 time, 30d respectively organizes and all adopts identical feeding and management method continuously.In the feeding process it is observed, and the poisoning clinical symptoms of each group of record.
2. to the mensuration of big white mouse growth body weight and immune organ index
Duration of test, every 15d weighs once, during off-test, weigh, ptomatopsia, and get spleen, thymus gland is weighed, and calculates index and spleen index and thymus index by following formula:
Index and spleen index or thymus index (mg/g)=spleen or thymic weight (mg)/body weight (g)
3. to the detection of big white mouse blood physiology biochemical indicator
In test 30d docking blood sampling, carry out red blood cell, white blood cell count(WBC), hemoglobin, albumin, total protein content are measured, and to alkaline phosphatase, glutamic-pyruvic transaminase, urea nitrogen, the active mensuration of serum cholesterol.Except that red blood cell and leucocyte adopt the full-automatic blood cell analyzer, all the other indexs all adopt the MD-100 automatic biochemistry analyzer to detect.
4. Histopathology variation characteristic
Cut open the dead and dying big white mouse of inspection in the process of the test, put to death all big white mouse during off-test, cut open inspection, get its internal organ and carry out histopathologic examination.The internal organ that at first detect by an unaided eye change, and core then, tissues such as lung, liver, kidney, intestines, use 10% formalin fixed, and wax is passed through in conventional dehydration, FFPE, and section, H.E dyes, om observation.
5. data statistics is handled and is used SPSS16.0 version, t method of inspection analysis bank differences.
6. result and analysis
6.1 influence to big white mouse body weight and immune organ index
Oral feeding during 15d, the body weight of processed group and control group there are no significant difference, during 30d, the 2nd group of also there was no significant difference (P>0.05) of comparing with control group compared significant difference (P<0.05) for the 3rd group, the 4th group with control group.The growth that can promote big white mouse is added in explanation in 50% ratio.When feeding 30d, the immune organ index of processed group all is higher than control group, significant difference (P<0.05).Explanation is along with the prolongation of feeding time, and this prescriptions of traditional Chinese medicine grain can promote the growth of immune organ, improves the immunity of body.
6.2 the detection of big white mouse blood physiology biochemical indicator
Feed to the big white mouse of 30d, prescriptions of traditional Chinese medicine grain group rises more obvious than its red blood cell of control group, leucocyte and content of hemoglobin, with control group comparing difference remarkable (P<0.05).
Total protein content and control group comparing difference be (P<0.05) significantly, and other indexs have no significant change, and compares with control group, and difference is not significantly (P>0.05) all.Explain that this prescriptions of traditional Chinese medicine grain of feeding does not all make significant difference to the activity and the physical signs of body sero-enzyme.
Annotate: * representes and the remarkable P of control group comparing difference<0.05; * representes extremely significantly P<0.01 of difference.
Subacute toxicity test is the result show; After adding this prescriptions of traditional Chinese medicine grain; Red blood cell, the leucocyte of big white mouse, the quantity of hemoglobin are increased to some extent; The immune organ index raises, and the big white mouse body weight is increased, and explains that this prescriptions of traditional Chinese medicine grain has enhancing body immunity and the effect that promotes growth of animal.This mainly is the immune system that the required immunologic active materials such as invertase of the alkaloid, glycoside, polysaccharide and the animal that contain in the traditional Chinese medicinal components can the activation body, has the immune organ of promotion like polysaccharide and grows, and improves the quantity and enhancing phagocytic activity of the leucocyte and the mononuclear macrophage of animal body; All influential to body specific immunity and nospecific immunity, cellular immunity and humoral immunity, glycoside can be strengthened the reticuloendothelial system phagocytic function, and can generate by enhancing antibody; Stimulator antigen antibody response and lymphocyte transformation etc.; These materials excite immune response from different links, comprising promoting immune organ to grow the enhance immunity cytoactive; Influence humoral immunity; Regulate cellular immunity, thereby improve the resistance infection of body, reach the purpose of preventing and curing diseases.Particularly contain various trace elements, vitamin and other bioactivator in this prescriptions of traditional Chinese medicine grain; Quickened animal metabolism; Make nutriment degraded fast in animal intestinal, promote the digesting and assimilating of nutritional labeling in the feed, and can improve the absorptivity of nutritional labeling greatly; Make it to produce collaborative mutually and overall balance effect to animal body; Greatly improve the utilization rate of feed, improve absorption and the conversion of animal, to reach the purpose that promotes that animal increases and practice thrift feed feed.
The safety testing of this prescriptions of traditional Chinese medicine grain shows that the pathological change of any poisoning symptom and tissue does not appear in big white mouse, and adds in 50% ratio and to feed, and also has somatotrophic effect.Therefore, this prescriptions of traditional Chinese medicine grain is safe, reasonable, effective nontoxic feed.
The mensuration of experiment two pairs of pet dog blood parameters, physical signs and Radical Metabolism
1. experimental animal and grouping
Select health for use through the pet dog breeding field, physically well develop, 60 of close beasle dogs of body weight, be divided into experimental group and control group at random, 30 every group, male and female half and half.The experimental group prescriptions of traditional Chinese medicine grain of feeding every day, the control group common feed for pet of feeding every day.
2. feeding and management
Test the full phase and only adopted single cage to feed, at regular time and quantity, freely drink water all trying dog.Feed period 60 days, feeding and management method is identical.
3. test method
3.1 routine blood test is measured: utilize full-automatic blood cell analysis-e/or determining, surveyed routine blood test in the 1st day, 30 days, 60 days.
3.2 the mensuration of serum physiological and biochemical index: utilize kit measurement T-CHOL, triglycerides, total protein, globulin, albumin.Total protein (TP): biuret (Biuret) method; Albumin (ALB): bromine cresols chlorine (BCG) method; Globulin (GLO): total protein deducts albumin; Triglycerides (TG): GPO (GPO) method; T-CHOL (CHO): cholesterol oxidase oxidation (CHOD) method.Used kit is available from Shanghai Rong Sheng technical research institute, and operation is undertaken by the kit specification.
3.3SOD, the mensuration of MDA, NO: just trying the 1st, 30,60 days phases in test and measuring SOD vigor, MDA content and NO level in the dog serum.Kit builds up bio-engineering research institute available from Nanjing, and operation is undertaken by the kit specification.
4. result of the test
4.1 prescriptions of traditional Chinese medicine grain is to the influence of dog blood physiology index
Annotate: * representes to compare P<0.05 with control group; * representes P<0.01.
The result shows that test initial stage control group and test group dog blood middle leukocytes content all are higher than the normal physiological scope, latter stage control group and experimental group pup leucocyte level (6.0-17.0 * 10 in normal range (NR) 9/ L).Experimental group pup leucocyte in normal range (NR) apparently higher than control group (P<0.01).
Test full phase control group and test group dog blood medium size lymphocyte content all is higher than normal physiological scope (0.8-5.1 * 10 9/ L).Experimental group pup lymphocyte in normal range (NR) apparently higher than control group (P<0.05).
Test full phase experimental group pup red blood cell and hemoglobin in normal range (NR) apparently higher than control group (P<0.01).
4.2 prescriptions of traditional Chinese medicine grain is to the influence of dog blood parameters
Annotate: * representes to compare P<0.05 with control group; * representes P<0.01.
The result shows that experimental group dog serum total protein (P<0.05), globulin (P<0.01), albumin (P<0.05) content are apparently higher than control group; Total cholesterol level is starkly lower than control group (P<0.01).
4.3 the free radical level of experimental dog changes
Annotate: * representes to compare P<0.05 with control group; * representes P<0.01.
Tested the 1st day, the SOD enzyme activity in test group and the control group pup serum does not have marked difference.Test group and control group 30 days SOD enzyme activity of test test the 1st day low; Test the 60th day SOD enzyme activity, test the 1st day low but test the 30th day high.Tested the 60th day, the SOD enzyme activity is then high by 7.18% than control group in the test group serum, significant difference (P<0.05).
Tested the 1st day, MDA content is higher by 27.63% than control group in the test group pup serum, and test the 30th day with test the 60th day, control group than test group respectively high 11.33% and 3.64%.Control group and test group be in test the 30th day the time, in the serum MDA content all test the 1st day high with examination the 60th day; Test in the 60th day serum MDA content all then test the 1st day low.
Tested the 1st day, test group pup serum NO level is higher by 6.19% than control group, and test the 30th day with test the 60th day, control group respectively than test group high 11.49% and 11.56%.Control group and test group be in test the 30th day the time, the serum NO level all test the 1st day high with test the 60th day.Control group is in the 60th day pup serum NO level level of test and the 1st day the horizontal no significant difference of NO; The 60th day serum NO level level of test group was then tested the 1st day low 14.79%.
60 pet dog in test end are disease-resistant, anti-stress ability obviously strengthens, and test the full phase and do not see the morbidity phenomenon, and the pet dog figure is healthy and strong, and expression is active, and appetite strengthens to some extent, and a dog hair luster sense is better, disease-resistant efficient be 100%.So this prescriptions of traditional Chinese medicine grain of feeding for a long time has humidification to the pet dog immunologic function.
The immunity enhancement type prescriptions of traditional Chinese medicine adult dogs grain of the present invention's development; Breeding observing through 2 years; Be applied to that 171 examples comprise since vaccine stress, change food stress, the pet dog of environmental stress and the born thin and weak immunocompromised that causes of physique; The result shows that wherein 158 examples show as effective immunologic hypofunction symptom that above-mentioned reason causes of having improved, and efficient is 92.34%.This product is balanced in nutrition; Pet dog appetite improves in section, and raise immunity is built up health and resistance against diseases; Have and holding up that this is solid just, invigorating the spleen is promoted the production of body fluid, the health protection and therapeutic action of liver-kidney tonifying; Can become after weak and sickly, postoperative rehabilitation, the ill rehabilitation and the only desirable auxiliary treating method of pet dog after the vaccine injection, and good palatability, be convenient to processing and fabricating and large-scale production.Supplemental treatment when being applicable to pet adult dogs infectious disease subclinical infection phase and anti-infective therapy also can be used as daily health caring prescription grain and is used to raise high-quality and becomes dog.

Claims (3)

1. prescriptions of traditional Chinese medicine dog grain that is applicable to adult dogs with immune enhancing function, comprising: weight ratio is 2: 1: 1: 0.5 fresh poultry meat, fish meal, bone meal and Normal juice hepar siccatum 20-40 weight portion, weight ratio are 1: 1 soyabean protein powder and dregs of beans 10-20 weight portion; Weight ratio is 1: 1 corn flour and wheat flour 20-40 weight portion, and weight ratio is 2: 1 vegetable oil and a deep sea fish oil 1-10 weight portion, and weight ratio is 2: 1 Vegetable powder and a dry tomato 1-6 weight portion; Spirulina 1-6 weight portion; Betaine 0.5-1.0 weight portion, salt 0.25-0.50 weight portion, white sugar 0.5-1.8 weight portion; Brewer's yeast 1-3 weight portion; Lactic acid bacteria 1-3 weight portion, microelement pre-mix material 0.5-1.0 weight portion, the weight ratio of Mg in the microelement pre-mix material: Fe: Cu: Zn: Mn: I equals 6.52: 5.07: 0.24: 5.26: 2.69: 0.65; Vitamin premix 0.5-1.0 weight portion, VB1 in the vitamin premix: riboflavin: pantothenic acid: niacin: VK: folic acid: biotin: VB 12: the weight ratio of choline: VA: VD: VE is 1.02: 1.04: 3.75: 3.95: 0.6925: 0.128: 1.25 weight portions: 0.50: 4.50: 1.25: 0.10: 10.00; It is characterized in that also having 5-15 parts by weight of Chinese traditional medicine compound in the dog grain, Chinese medicine is wherein formed and weight ratio is: the Radix Astragali: windproof: Poria cocos: the bighead atractylodes rhizome: Radix Angelicae Sinensis: the fruit of Chinese magnoliavine: Radix Glycyrrhizae equals 80-110: 15-30: 10-30: 15-25: 30-40: 20-40: 5-15.
2. the prescriptions of traditional Chinese medicine dog grain that is applicable to adult dogs with immune enhancing function according to claim 1, it is characterized in that wherein also having weight ratio is 1: 1 beef flavor and chicken essence 0.20-0.50 weight portion.
3. the prescriptions of traditional Chinese medicine dog grain that is applicable to adult dogs with immune enhancing function according to claim 1 and 2 is characterized in that also being added with in addition in the dog grain 0.3% mould inhibitor.
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CN104686869A (en) * 2015-04-03 2015-06-10 王经凤 Dog food with resistance enhancing function
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CN109527219A (en) * 2019-01-23 2019-03-29 湖南农业大学 The nutritional supplementation grain and its application method of a kind of extract containing ciliate desert-grass and application
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