CN102696908B - Chinese medicinal dog food with function of enhancing immunity - Google Patents
Chinese medicinal dog food with function of enhancing immunity Download PDFInfo
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Abstract
The invention discloses a healthcare food for adult pet dogs, which is prepared by adding a certain amount of a group of Chinese medicinal compounds which comprise astragalus mongholicus, saposhnikovia divaricata, poria, atractylodes, angelica, schisandra and licorice into a common food for pets. The healthcare food for adult pet dogs has the healthcare efficacy of strengthening the body resistance to consolidate the constitution, invigorating the spleen, promoting the secretion of saliva or body fluid and tonifying the lever and the kidney and the functions of increasing the appetite of pets, improving the capacity of resisting a variety of diseases and reducing the stress response and enhancing immunity. The healthcare food can be fed to the adult pet dogs in the subclinical infection period of infectious diseases to assist to the anti-infection treatment.
Description
Technical field:
The present invention relates to a kind of become the prescription grain of dog, particularly a kind of pet becomes the health food of dog.
Background technology:
At present, animal with treatment medicine mainly for infectious diseases.Antibiotic, antibacterial chemical, anti parasitic antibiotic, chemicals are all that pharmaceutically-active object target spot is locked in (as bacteria cell wall, bacterial ribosome etc.) in pathogen without exception, and have ignored the utilization of the phylactic power defensive power of animal body self.Since 20th century, the physician of not rare strategic perspective clearly proposes that " therapeutic first principle is the utilization of spontaneous recovery power." relative to antibiotic therapy method; modern medical service pattern becomes at present from simple disease therapy the Therapeutic mode that " prevent, control, support " combine; the development trend of novel therapeutic method is the reaction of non-specific enhancing innate immunity, to improve the immune defense ability of body.
Research in recent years confirms, nutrition, disease and immune three are reciprocal causations, complementary.Nutrition can improve the response of body to disease and the immunologic function of patient, and disease can cause malnutrition and immune deficiency, and immunity can affect the utilization of body to nutrient matrix and the prognosis to disease has an impact.In order to adapt to the needs of pet clinical treatment, in some developed countries such as America and Europes, pet prescription grain has become indispensable supplemental treatment measure when treating some diseases.
Existing dog grain can only provide the daily required energy of dog and basic nutrition element, but does not act on raising dog immunologic function and minimizing pet stress reaction.
Summary of the invention
The invention provides and a kind ofly overcome prior art deficiency, have and holding up that this is solid just, invigorating the spleen is promoted the production of body fluid, effect of liver-kidney tonifying, can immunologic function be strengthened, improve resistance against diseases, reduce the one-tenth dog prescriptions of traditional Chinese medicine grain of adult pet dog stress reaction.
Include in prescriptions of traditional Chinese medicine dog grain of the present invention: weight ratio is the fresh poultry meat of 2: 1: 1: 0.5, fish meal, bone meal and Normal juice hepar siccatum 20-40 weight portion, weight ratio is soyabean protein powder and the dregs of beans 10-20 weight portion of 1: 1, weight ratio is 1: 1 corn flour and wheat flour 20-40 weight portion, weight ratio is vegetable oil and the deep sea fish oil 1-10 weight portion of 2: 1, weight ratio is Vegetable powder and the dry tomato 1-6 weight portion of 2: 1, spirulina 1-6 weight portion, betaine 0.5-1.0 weight portion, salt 0.25-0.50 weight portion, white sugar 0.5-1.8 weight portion, brewer's yeast 1-3 weight portion, lactic acid bacteria 1-3 weight portion, microelement pre-mix material 0.5-1.0 weight portion, in microelement pre-mix material, the weight ratio of Mg: Fe: Cu: Zn: Mn: I equals 6.52: 5.07: 0.24: 5.26: 2.69: 0.65, vitamin premix 0.5-1.0 weight portion, VB1 in vitamin premix: riboflavin: pantothenic acid: niacin: VK: folic acid: biotin: VB
12: choline: the weight ratio of VA: VD: VE is 1.02: 1.04: 3.75: 3.95: 0.6925: 0.128: 1.25: 0.50: 4.50: 1.25: 0.10: 10.00.Except aforementioned component, also have the Chinese medicine compound prescription of 5-15 weight portion in dog grain of the present invention, Chinese medicine composition wherein and weight ratio are: the Radix Astragali: windproof: Poria cocos: the bighead atractylodes rhizome: Radix Angelicae Sinensis: the fruit of Chinese magnoliavine: Radix Glycyrrhizae equals 80-110: 15-30: 10-30: 15-25: 30-40: 20-40: 5-15.
The present invention is applicable to also to add beef flavor and the chicken essence 0.20-0.50 weight portion that weight ratio is 1: 1 in the prescriptions of traditional Chinese medicine dog grain of adult dogs.
Of the present inventionly be applicable to the mould inhibitor that also separately can be added with 0.3% in the prescriptions of traditional Chinese medicine dog grain of adult dogs.
From above content, the present invention adds a certain amount of one group of Chinese medicine compound prescription in conventional pet grain, this pet grain is had holding up that this is solid just, invigorating the spleen is promoted the production of body fluid, effect of liver-kidney tonifying, pet food wish can be increased, improve the ability of pet opposing various diseases, reduce pet stress reaction (vaccine stress, change food stress, environmental stress etc.), strengthen the function of immunity.This prescriptions of traditional Chinese medicine grain is applicable to supplemental treatment when adult dogs infectious disease subclinical infection phase and anti-infective therapy.
Modern Chinese medicine pharmacy result of study shows, the active component coming from Chinese medicine has the double action of nutrient and pharmacologically active concurrently.Traditional Chinese medicine has a long history and rich experience in health care.Based on this, animal nutrition, traditional Chinese veterinary medicine and Chinese medicine immunopharmacology creatively carry out integrating and innovating by the present invention, define the prescriptions of traditional Chinese medicine grain product for core, following " food and medicine consangunity " principle with " origin conditioning ".Its starting point is to pay close attention to pet health growth.
The prescriptions of traditional Chinese medicine dog grain that the present invention relates to, according to modem animal nutrient theory, on the Research foundation of strengthening pet food nutrient balance, under Chinese veterinarian's theoretical direction, carry out prescription, by adjusting the trophic structure of pet food, utilization has invigorating qi for consolidating superficies, nourishing liver and kidney, the Chinese medicine compound prescription that invigorating the spleen is promoted the production of body fluid, build the prescriptions of traditional Chinese medicine grain of conditioning pet dog body autoimmunity vis medicatrix naturae, its compatibility takes into full account the palatability of finished product, the demand of Acidity of Aikalinity and Nutrition and Metabolism, creationaryly traditional Chinese medicine and pet food are carried out integrating and improve, it is the product that traditional traditional Chinese veterinary medicine and pet clinical nutriology combine, the best agent of " food and medicine consangunity " theory, by the adjustment of dietary structure and comprehensive opsonic action of Chinese medicine compound prescription, action target spot is locked in the immune organ of host, start, Promote immunity cell development, differentiation, ripe, the generation of Promote immunity responsing reaction, recover normal physiological condition (rehabilitation, heilen).Be specially adapted to supplemental treatment when infectious disease subclinical infection phase and anti-infective therapy.The development of prescriptions of traditional Chinese medicine grain provides best annotation by making rival claims as an equal to similar domestic brand and import brand.
Due to having a sharp sense of smell of dog, the appetite of dog may be affected to a certain extent after adding traditional Chinese medicine ingredients, the present invention is according to the having a sharp sense of smell of dog, feature that taste function is more weak, a certain proportion of beef flavor, chicken essence is with the addition of through repetition test, effectively can cushion flavour of a drug, strengthen the appetite of pet.
The present invention has the mould inhibitor being applicable to also can separately be added with in the prescription dog grain of adult dogs 0.3%Wt of immune enhancing function, makes pet grain of the present invention can have longer storage period.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
(1) first prepare basal nutrient daily ration, its basic composition adopts following weight proportioning (in 1000g basal diet):
Fresh poultry meat, fish meal, bone meal and Normal juice hepar siccatum 20-40 weight portion (fresh poultry meat, fish meal, the weight ratio of bone meal and Normal juice hepar siccatum is 2: 1: 1: 0.5), soyabean protein powder and dregs of beans 10-20 weight portion (weight ratio of soyabean protein powder and dregs of beans is 1: 1), corn flour and wheat flour 20-40 weight portion (weight ratio of corn flour and wheat flour is 1: 1), (weight ratio of vegetable oil and deep sea fish oil is 2: 1 for vegetable oil and deep sea fish oil 1-10 weight portion, Vegetable powder and dry tomato 1-6 weight portion (weight ratio of Vegetable powder and dry tomato is 2: 1), spirulina 1-6 weight portion, betaine 0.5-1.0 weight portion, salt 0.25-0.50 weight portion, beef flavor, chicken essence 0.20-0.50 weight portion (weight ratio 1: 1 of beef flavor and chicken essence), sucrose 0.5-1.8 weight portion, brewer's yeast 1-3 weight portion, lactic acid bacteria 1-3 weight portion, microelement pre-mix material 0.5-1.0 weight portion (in microelement pre-mix material, the weight ratio of Mg: Fe: Cu: Zn: Mn: I equals 6.52: 5.07: 0.24: 5.26: 2.69: 0.65), vitamin premix 0.5-1.0 weight portion (VB1: riboflavin: pantothenic acid: niacin: VK: folic acid: biotin: VB
12: choline: the weight ratio of VA: VD: VE is 1.02: 1.04: 3.75: 3.95: 0.6925: 0.128: 1.25: 0.50: 4.50: 1.25: 0.10: 10.00).Each component is mixed, stirs stand-by.
(2) prepare 5-15 weight portion Chinese medicine compound prescription, its basic composition and weight proportion as follows:
Chinese medicine composition wherein and weight proportion are: Radix Astragali 80-110 weight portion, windproof 15-30 weight portion, Poria cocos 10-30 weight portion, bighead atractylodes rhizome 15-25 weight portion, Radix Angelicae Sinensis 30-40 weight portion, fruit of Chinese magnoliavine 20-40 weight portion, Radix Glycyrrhizae 5-15 weight portion.
By above each component ultramicro grinding, 200 orders sieve, and mix that to make powder stand-by.
(3) in basal nutrient daily ration, add Chinese medicine compound prescription powder, add the mould inhibitor of 0.3% elsewhere, after mix and blend, granulation is carried out expanded, makes expanded pellet feed.
Zoopery
Test a safety evaluatio and subchronic toxicity test
1. animal grouping and feed
Wistar rat, body weight 200 ~ 240g, male and female half and half, purchased from Lanzhou Institute of Biological Products.40 rats are divided into 4 groups at random, often organize 10, male and female half and half.1st group is control group, basal nutrient daily ration of feeding every day; 2nd, 3,4 groups is processed group, and add prescriptions of traditional Chinese medicine grain in the ratio of 25%, 50%, 100% successively, every day 1 time, continuous 30d, each group all adopts identical feeding and management method.In feeding process, it is observed, and record the poisoning clinical symptoms of each group.
2. the mensuration of pair big white mouse growth body weight and Immune Organs Index
Duration of test, every 15d weighs once, during off-test, weighs, ptomatopsia, and get spleen, thymus gland is weighed, and calculates index and spleen index and thymus index as follows:
Index and spleen index or thymus index (mg/g)=spleen or thymic weight (mg)/body weight (g)
3. the detection of pair big white mouse blood physiological and biochemical indices
Dock at test 30d and take a blood sample, carry out red blood cell, white blood cell count(WBC), hemoglobin, albumin, total protein content measure, and the mensuration to alkaline phosphatase, glutamic-pyruvic transaminase, urea nitrogen, serum cholesterol activity.Except red blood cell and leucocyte adopt full-automatic blood cell analysis machine, all the other indexs all adopt MD-100 automatic biochemistry analyzer to detect.
4. Histopathologic changes feature
Cut open inspection death and dying big white mouse in process of the test, put to death all big white mouse during off-test, carry out cuing open inspection, get its internal organ and carry out histopathologic examination.First the internal organ that detect by an unaided eye change, then core, lung, liver, kidney, the tissue such as intestines, fix with 10% formalin, conventional dehydration, saturating wax, FFPE, section, H.E dyeing, om observation.
5. data statistics processing SPSS16.0 version, t method of inspection analyzes group difference.
6. results and analysis
6.1 impacts on big white mouse body weight and Immune Organs Index
During oral Gavage 15d, the body weight of processed group and control group there are no significant difference, during 30d, 2nd group of also there was no significant difference (P > 0.05) compared with control group, 3rd group, the 4th group compared with control group, significant difference (P < 0.05).Illustrate to carry out adding in 50% ratio and can promote the growth of big white mouse.Feed 30d time, the Immune Organs Index of processed group all higher than control group, significant difference (P < 0.05).Prolongation along with feeding time is described, the growth of this prescriptions of traditional Chinese medicine grain energy Promote immunity organ, improves the immunity of body.
The detection of 6.2 big white mouse blood physiological and biochemical indices
Feed to the big white mouse of 30d, prescriptions of traditional Chinese medicine grain group comparatively its red blood cell of control group, leucocyte and content of hemoglobin rises comparatively obvious, with control group comparing difference remarkable (P < 0.05).
Significantly (P < 0.05), other indexs have no significant change for total protein content and control group comparing difference, and compared with control group, difference is not all significantly (P > 0.05).Illustrate that this prescriptions of traditional Chinese medicine grain of feeding all does not make significant difference to the activity of body sero-enzyme and physical signs.
Note: * represents the remarkable P < 0.05 with control group comparing difference; * represents difference extremely significantly P < 0.01.
Subacute toxicity test result shows, after adding this prescriptions of traditional Chinese medicine grain, the quantity of the red blood cell of big white mouse, leucocyte, hemoglobin can be made to increase to some extent, Immune Organs Index raises, and big white mouse body weight can be made to increase, illustrate that this prescriptions of traditional Chinese medicine grain has the effect strengthening immunity of organisms and promote growth of animal.This alkaloid mainly contained in traditional Chinese medicinal components, glycoside, the immune system of the immunity substance mass-energy activation equipment bodies such as polysaccharide and the invertase needed for animal, as polysaccharide has Promote immunity allelotaxis, improve the leucocyte of animal body and the quantity of mononuclear macrophage and strengthen phagocytic activity, to body specific immunity and nospecific immunity, cellular immunity and humoral immunity all have impact, glycoside can strengthen reticuloendothelial system phagocytic function, and can generate by enhancing antibody, stimulator antigen antibody response and lymphocyte transformation etc., these materials excite immune response from different link, comprising Promote immunity allelotaxis, strengthen immunologic cellular activity, affect humoral immunity, regulate cellular immunity, thus improve the resistance infection of body, reach the object of preventing and curing diseases.Particularly contain various trace elements, vitamin and other bioactivator in this prescriptions of traditional Chinese medicine grain, accelerate animal metabolism, make nutriment fast degradation in animal intestinal, promote digesting and assimilating of the nutritional labeling in feed, and greatly can improve the absorptivity of nutritional labeling, make it to produce to animal body collaborative and overall balance effect mutually, greatly improve the utilization rate of feed, improve animal to the absorption of feed and conversion, promote that animal increases and saves the object of feed to reach.
The safety testing of this prescriptions of traditional Chinese medicine grain shows, the pathological change of any poisoning symptom and tissue does not appear in big white mouse, and carries out interpolation in 50% ratio and feed, and also has somatotrophic effect.Therefore, this prescriptions of traditional Chinese medicine grain is safe, reasonable, effective nontoxic feed.
The mensuration of experiment two pairs of pet dog blood parameters, physical signs and Radical Metabolism
1. experimental animal and grouping
Select health through pet dog breeding field, physically well develop, the close beasle dog of body weight 60, be divided into experimental group and control group at random, often organize 30, male and female half and half.Experimental group to be fed prescriptions of traditional Chinese medicine grain every day, and control group to be fed average pet feed every day.
2. feeding and management
Testing the full phase only adopts single cage to feed to whole tested dog, at regular time and quantity, freely drinks water.Feed period 60 days, feeding and management method is identical.
3. test method
3.1 routine blood tests measure: utilize full-automatic blood cell analysis machine to measure, and survey routine blood test in the 1st day, 30 days, 60 days.
The mensuration of 3.2 Physiological-biochemical Parameters: utilize kit measurement T-CHOL, triglycerides, total protein, globulin, albumin.Total protein (TP): biuret (Biuret) method; Albumin (ALB): bromine cresols chlorine (BCG) method; Globulin (GLO): total protein deducts albumin; Triglycerides (TG): GPO (GPO) method; T-CHOL (CHO): cholesterol oxidation oxydasis (CHOD) method.Used kit, purchased from Shanghai Rong Sheng technical research institute, operates and is undertaken by kit description.
The mensuration of 3.3SOD, MDA, NO: just trying SOD vigor, MDA content and NO level in the 1st, 30,60 days phases mensuration dog serum in test.Kit builds up Bioengineering Research Institute purchased from Nanjing, operates and is undertaken by kit description.
4. result of the test
4.1 prescriptions of traditional Chinese medicine grains are on the impact of dog Blood Physiological Indexes
Note: * represents and compares P < 0.05 with control group; * represents P < 0.01.
Result shows, test initial stage control group and test group dog blood middle leukocytes content all higher than normal physiologic range, latter stage control group and leucocyte level (6.0-17.0 × 10 in normal range (NR) of experimental group pup
9/ L).Experimental group pup leucocyte in normal range (NR) apparently higher than control group (P < 0.01).
Test full phase control group and test group dog lymphocytes in blood content all higher than normal physiologic range (0.8-5.1 × 10
9/ L).Experimental group pup lymphocyte in normal range (NR) apparently higher than control group (P < 0.05).
Test full phase experimental group pup red blood cell and hemoglobin in normal range (NR) apparently higher than control group (P < 0.01).
4.2 prescriptions of traditional Chinese medicine grains are on the impact of dog blood parameters
Note: * represents and compares P < 0.05 with control group; * represents P < 0.01.
Result shows, experimental group dog serum total protein (P < 0.05), globulin (P < 0.01), albumin (P < 0.05) content are apparently higher than control group; Total cholesterol level is starkly lower than control group (P < 0.01).
The Free Radical Level change of 4.3 experimental dogs
Note: * represents and compares P < 0.05 with control group; * represents P < 0.01.
Test the 1st day, the SOD enzyme activity in test group and control group pup serum does not have marked difference.Test group and control group the test SOD enzyme activity of 30 days comparatively test the 1st day low; Test the SOD enzyme activity of the 60th day, comparatively test the 1st day low but comparatively test the 30th day high.Test the 60th day, in test group serum, then comparatively control group is high by 7.18% for SOD enzyme activity, significant difference (P < 0.05).
Test the 1st day, in test group pup serum, MDA content is higher than control group by 27.63%, and tests the 30th day and test the 60th day, and control group distinguishes height 11.33% and 3.64% than test group.Control group and test group when test the 30th day, in serum MDA content all comparatively test the 1st day and examination the 60th day high; To test in the 60th day serum MDA content all then comparatively test the 1st day low.
Test the 1st day, test group pup serum NO level is higher than control group by 6.19%, and tests the 30th day and test the 60th day, and control group is higher than test group respectively 11.49% and 11.56%.Control group and test group when test the 30th day, serum NO level all comparatively test the 1st day and test the 60th day high.Control group is in the horizontal no significant difference of NO of the test pup serum NO level level of the 60th day and the 1st day; The test group serum NO level level of the 60th day then comparatively test the 1st day low by 14.79%.
60 pet dog in test end are disease-resistant, anti-stress ability obviously strengthens, and test the full phase to have no morbidity phenomenon, and pet dog figure is healthy and strong, and expression is active, and appetite strengthens to some extent, a dog hair luster sense better, disease-resistant efficient be 100%.Therefore this prescriptions of traditional Chinese medicine grain of Long-term Feeding has humidification to pet dog immunologic function.
The immunity enhancement type prescriptions of traditional Chinese medicine adult dogs grain of the present invention's development, through the breeding observing of 2 years, be applied to 171 examples comprise due to vaccine stress, change food stress, the pet dog of environmental stress and the born thin and weak immunocompromised caused of physique, result shows, wherein 158 examples show as the immunologic hypofunction symptom effectively improving above-mentioned reason and cause, and efficient is 92.34%.This product is balanced in nutrition; pet dog appetite improves in section; strengthen immunologic function; build up health and resistance against diseases; have and holding up that this is solid just, invigorating the spleen is promoted the production of body fluid, the health protection and therapeutic action of liver-kidney tonifying; can become weak and sickly, auxiliary treating method that postoperative rehabilitation, pet dog after ill rehabilitation and after vaccine injection are only desirable, and good palatability, is convenient to processing and fabricating and large-scale production.Be applicable to supplemental treatment when pet adult dogs infectious disease subclinical infection phase and anti-infective therapy, also can be used as daily health caring prescription grain and become dog for raising high-quality.
Claims (1)
1. one kind has the prescriptions of traditional Chinese medicine dog grain being applicable to adult dogs of immune enhancing function, comprise: weight ratio is the fresh poultry meat of 2:1:1:0.5, fish meal, bone meal and Normal juice hepar siccatum 20-40 weight portion, weight ratio is soyabean protein powder and the dregs of beans 10-20 weight portion of 1:1, weight ratio is corn flour and the wheat flour 20-40 weight portion of 1:1, weight ratio is vegetable oil and the deep sea fish oil 1-10 weight portion of 2:1, weight ratio is Vegetable powder and the dry tomato 1-6 weight portion of 2:1, spirulina 1-6 weight portion, betaine 0.5-1.0 weight portion, salt 0.25-0.50 weight portion, white sugar 0.5-1.8 weight portion, brewer's yeast 1-3 weight portion, lactic acid bacteria 1-3 weight portion, microelement pre-mix material 0.5-1.0 weight portion, in microelement pre-mix material, the weight ratio of Mg: Fe: Cu: Zn: Mn: I equals 6.52: 5.07: 0.24: 5.26: 2.69: 0.65, vitamin premix 0.5-1.0 weight portion, VB in vitamin premix
1: riboflavin: pantothenic acid: niacin: VK: folic acid: biotin: VB
12: choline: the weight ratio of VA: VD: VE is 1.02: 1.04: 3.75: 3.95: 0.6925: 0.128: 1.25: 0.50: 4.50: 1.25: 0.10: 10.00, it is characterized in that the Chinese medicine compound prescription also having 5-15 weight portion in dog grain, the Chinese medicine composition of this Chinese medicine compound prescription and weight ratio are: the Radix Astragali: windproof: Poria cocos: the bighead atractylodes rhizome: Radix Angelicae Sinensis: the fruit of Chinese magnoliavine: Radix Glycyrrhizae equals 80-110: 15-30: 10-30: 15-25: 30-40: 20-40: 5-15.
2. the prescriptions of traditional Chinese medicine dog grain being applicable to adult dogs with immune enhancing function according to claim 1, is characterized in that wherein weight ratio is beef flavor and the chicken essence 0.20-0.50 weight portion of 1:1 in addition.
3. the prescriptions of traditional Chinese medicine dog grain being applicable to adult dogs with immune enhancing function according to claim 1 and 2, is characterized in that the mould inhibitor being also separately added with 0.3% in dog grain.
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