CN106689818A - Aquatic product immunopotentiator based on salicornia bigelovii torr resource utilization - Google Patents
Aquatic product immunopotentiator based on salicornia bigelovii torr resource utilization Download PDFInfo
- Publication number
- CN106689818A CN106689818A CN201611239681.2A CN201611239681A CN106689818A CN 106689818 A CN106689818 A CN 106689818A CN 201611239681 A CN201611239681 A CN 201611239681A CN 106689818 A CN106689818 A CN 106689818A
- Authority
- CN
- China
- Prior art keywords
- parts
- glasswort
- fermentation
- mass ratio
- immunopotentiator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses an aquatic product immunopotentiator based on salicornia bigelovii torr resource utilization. The aquatic product immunopotentiator has the multiple effects of prevention, health care, treatment, immunoregulation and the like. The aquatic product immunopotentiator is an ideal, green, environment-friendly, safe and novel feed additive replacing antibiotic drugs. The immunopotentiator is prepared by Chinese herbal medicines including salicornia bigelovii torr, acanthopanax root, rhodiola rosea, Gorgon euryale seeds, medicated leaven, oriental wormwood, platycodon grandiflorum, liquorice roots, radix scrophulariae, fructus amomi, herba eupatorii and the like through a 'enzyme extraction, ultrasonic wave assisted extraction, multi-strain aerobic anaerobic two-step fermentation' process, has the multiple advantages of the salicornia bigelovii torr, the Chinese herbal medicines and probiotics, can effectively regulate intestinal health of grass carps, snakeheaded fish, carp and other aquaculture animals, treats both symptoms and root causes, reinforces the vital essence, strengthens the primordial qi, keeps microecological balance of the intestinal tract and a best immune state, improves digestion and absorption rate of nutrient substances, improves immunity of the organism, improves meat quality and aquaculture earnings and reduces aquaculture risk. The problems of drug residues, drug resistance and the like do not exist, and the aquatic product immunopotentiator has a wide market prospect.
Description
Technical field
The present invention relates to a kind of aquatic immune reinforcing agent based on glasswort recycling, belong to feed addictive neck
Domain.
Background technology
In order to meet the demand of ever-increasing aquatic products, solve constantly to expand because of aquaculture scale and growth cycle contracting
Physiological disorders, disease take place frequently the Important Problems as aquaculture caused by subtracting, and are also aquatic feeds industry development
It is crucial.In this context, antibioticses feed addictive is subject to heat to hold in both hands, and is widely used in feed industry, to a certain extent
Promote the development of culture fishery, but antibiotic bring high residue, high pollution and the resistance to the action of a drug the problems such as more serious.
Develop the environment-friendly feed additives such as probiotics, Chinese medicine immunity enhancer successively at present, but still suffer from effect
Effect does not know, be affected by the external environment the drawback such as larger, and especially for fish, Chinese medicine immunity enhancer has palatability
The drawback such as difference, water pollution be serious, therefore be beneficial to further push away based on ocean or alkaline development of resources novel immune reinforcing agent
Enter the development of ecological aquaculture aquaculture.
Glasswort belongs to jasmine section plant, glasswort category, in salt life also known as extra large asparagus, extra large pilose antler, marine borer grass, rich and honour dish etc.
In plant classification, belong to stem carnification euhalophyte, be more common in marshland, salt lake side and and seabeach.Glasswort its dry plant
Strain containing cellulose 5~20%, protein 9.0%, rich in 8 kinds of amino acid needed by human, trace element and vitamin, wherein recklessly
Radish cellulose content exceedes 40 times of common vegetable, also containing natural plant health salt and natural vegetable soda (micro- angle saponin(e), tool
There is the effects such as reduction cholesterol, step-down.The plantation of glasswort has significant application value to improving China's saline-alkali environment, but at present
The comprehensive development and utilization degree of glasswort is relatively low, governs glasswort secondary industry economic benefit, also directly affects glasswort
Plantation promote, therefore, if glasswort is carried out into high-valued comprehensive development and utilization, important tribute will be made to environment and economy
Offer.
The content of the invention
It is an object of the invention to according to above shortcomings in the prior art, the idea of development based on recycling economy,
Terrestrial Soiline-alkali plants resource glasswort is developed using modern biotechnology and prepares aquatic immune reinforcing agent, be remarkably improved water
The immunity of organisms of product, improvement growth of animal performance, reduce feed coefficient, reduce antibiotic and largely use the aquaculture for causing
Pollution, and raw material sources are wide, it is cheap, it is a kind of green, environmentally friendly, efficient feed addictive, develop one kind and be based on
The aquatic immune reinforcing agent of glasswort recycling.
Above-mentioned purpose of the present invention is achieved by the following technical programs：
A kind of aquatic immune reinforcing agent based on glasswort recycling, it is characterised in that by the raw material of following mass ratio
It is made：30~50 parts of Salicornia Bigelovii Torr. Powder, 15~20 parts of wilsonii, 8~12 parts of rhodiola root, 8~12 parts of Gorgon fruit, 6~8 parts of Divine Comedy, bacterium
Old 3~5 parts, 6~8 parts of balloonflower root, 6~8 parts of Radix Glycyrrhizae, 3~5 parts of radix scrophulariae, 5~7 parts of fructus amomi, 3~5 parts of eupatorium.
A kind of aquatic immune reinforcing agent based on glasswort recycling, it is characterised in that the raw material composition is preferred
For：40 parts of Salicornia Bigelovii Torr. Powder, 18 parts of wilsonii, 10 parts of rhodiola root, 10 parts of Gorgon fruit, 7 parts of Divine Comedy, old 4 parts of bacterium, 7 parts of balloonflower root, Radix Glycyrrhizae 7
Part, 4 parts of radix scrophulariae, 6 parts of fructus amomi, 4 parts of eupatorium.
The present invention provides the technique that a kind of bioanalysis prepares aquatic immune reinforcing agent using glasswort, and the Salicornia Bigelovii Torr. Powder is pre-
Handling process is as follows：
(1) immersion is crushed：Deimpurity glasswort cane will be gone to crush, cross 100 mesh sieves, be subsequently adding 2 times of mass ratioes pure
Water purification soaks 3~5h；
(2) boiling：By the glasswort suspension 1~2h of boiling after immersion, then decline in air-proof condition and warm to room temperature；
(3) separate：Cooking liquor is carried out into separation of solid and liquid, glasswort cane powder is obtained, the water after separation is carried out into step (1)
Recycle；
(4) become thoroughly decomposed naturally：Control glasswort cane powder water content 40~50%, 30~50 DEG C of temperature control, become thoroughly decomposed 30 naturally~
50d；
(5) two-step fermentation enzymolysis：Naturally become thoroughly decomposed after end, add glasswort mass ratio 2% cellulase and 1% it is non-
Starch-polysaccharides enzyme, and be inoculated with the salt tolerant Alkaliphilic bacillus of glasswort mass ratio 2~3%, ferment 3 under the conditions of 28~35 DEG C~
5d, stirring in 12 hours is once；Glasswort yeast powder is obtained final product after fermentation ends.
The present invention provides the technique that a kind of bioanalysis prepares aquatic immune reinforcing agent using glasswort, it is characterised in that including
Following steps：
(1) co-grinding：Each raw material is mixed in proportion, 100 mesh sieves were then crushed；Add the pure of 3 times of material quality
Water purification；
(2) digest：Cellulase, 2~3% non-starch polysaccharide enzyme of herbal raw material mass ratio 3~5% are added,
Temperature control digests 4~8h under the conditions of 40~60 DEG C, every 0.5h stirrings once；
(3) it is separated by filtration：Filtering enzymolysis liquid, obtains enzymolysis liquid and enzymolysis residue；
(4) Ultrasonic Wave-Assisted Extraction：To the pure water that 3~5 times are added in enzymolysis residue, under ultrasonic assistant effect,
Flow back extraction 20~30min, ultrasonic power 400w, frequency 25kHz under the conditions of 100 DEG C；Ultrasonic power 400w, frequency 25kHz；
(5) it is separated by filtration：Filtering leaching liquor, obtains leaching liquor and leaching residue；
(6) prepared by zymotic fluid：To 5 times of pure water of mass ratio of addition in the leaching residue in step (5), and add residue
The glucose of mass ratio 3.0~5.0%, 2.0~3.0% yeast extracts, 0.3~0.5% potassium dihydrogen phosphate, 0.02%MgSO4、
0.01%CaCl2, pH is adjusted to 7.0~7.2；
(7) aerobic fermentation：From liquid fermentation tank, liquid amount volume ratio is 60~70%, is inoculated with the sugared bacillus of cold solution
And aspergillus niger, inoculum concentration is respectively 2~4%, 1~2%, controls 28~37 DEG C of fermentation temperature, 150~300rpm of rotating speed；Herein
Ferment 12~24h under fermentation condition；
(8) anaerobic fermentation：After aerobic fermentation terminates, streptococcus thermophilus and Bacillus acidi lactici are inoculated with, inoculum concentration is 2~4%,
28~37 DEG C of fermentation temperature, 12~24h of temperature control anaerobic fermentation；
(9) merge：Enzymolysis liquid in step (3), the zymotic fluid in leaching liquor and step (8) in step (5) are merged；
(10) low temperature concentration：Enhancing agent solution after step (9) is merged under cryogenic is concentrated；
(11) it is adsorbed by drying：Adsorbed with 1~1.5 times of wheat bran of mass ratio after concentration, in 40~60 DEG C of blast driers
Dry, moisture is controlled 8~10%；
(12) crush：Dried product is sieved with the mesh of pulverizer 60 and is crushed, obtain final product aquatic immune reinforcing agent.
The present invention provides a kind of aquatic immune reinforcing agent based on glasswort recycling, with prevention, health care, treatment
The multiple efficacies such as immune with regulation, are the preferable, green, environmentally friendly of substitute antibiotics medicine, safety, novel fodder additive.Should
Invention immunopotentiator one can be efficiently to nurse one's health aquiculture animal intestinal health, treat both principal and secondary aspect of disease, and reinvigoration keeps enteron aisle
Microecological balance and optimal immune state, improve the digestibility of nutriment, improve animal body immunity；Two is to press down
The generation of the harmful bacteria such as system and prevention cause of disease coliform, clostridium, salmonella, increases beneficial bacteria of intestinal tract, remains strong
The gut flora of health；Three is enhancing immunization, by stimulating siberian crabapple cell in enteron aisle, increases the formation of local antibody, is increased
Plus macrophage activity；Four is that immunopotentiator enters enteral resistance harmful bacteria, reduces stench and harmful components are formed, and makes discharge
Excrement eliminate stench, protect breeding environment while improve aquiculture animal meat quality.
The present invention provides a kind of aquatic immune reinforcing agent based on glasswort recycling, the aquatic immune reinforcing agent
The mass ratio that product is used in aquatic feeds complete diet pellet is 0.1~0.5%：Or described feed addictive premix,
Concentrate feed is used with the ratio addition needed for complete diet pellet.
The present invention provides a kind of aquatic immune reinforcing agent based on glasswort recycling, it is advantageous that：One be by
According to the combination principle of Chinese herbal medicine monarch, with terrestrial Soiline-alkali plants glasswort as monarch drug in a prescription, it is aided with wilsonii, rhodiola root, Gorgon fruit
It is ministerial drug, with Divine Comedy, old bacterium, balloonflower root, radix scrophulariae, fructus amomi, eupatorium as adjutant, with Radix Glycyrrhizae as medicine is made, the effect of concurrence complements each other；
Especially glasswort is rich in natural plant health salt and natural vegetable soda (micro- angle saponin(e), more traditional to aquaculture fish
Herbal health care effect is more preferably；Two is to employ " enzymolysis and extraction+Ultrasonic Wave-Assisted Extraction+many in immunopotentiator preparation process
The production technology of strain aerobic-anaerobic fermentation two-step fermentation ", the action effect compared with existing Chinese herbal feed additive product
It is more notable；Three is the multiple advantage that immunopotentiator has glasswort, Chinese herbal medicine and probiotics etc., can efficiently nurse one's health aquatic products and support
Animal intestinal tract health is grown, is treated both principal and secondary aspect of disease, reinvigoration keeps intestinal microecology balance and optimal immune state, improves nutrients
The digestibility of matter, improves animal body immunity, improves cultivation income, reduces cultivation risk；Four is that the product is alternative
Antibiotic usage, has no adverse effects to aquiculture animal, using safety, the problems such as in the absence of medicament residue and drug resistance, has
Wide market prospects.
Specific embodiment
Embodiment 1：
A kind of careless immunostimulant for fish based on glasswort recycling, specific preparation process is as follows：
(1) immersion is crushed：Deimpurity glasswort cane will be gone to crush, cross 100 mesh sieves, be subsequently adding 2 times of mass ratioes pure
Water purification soaks 4h；
(2) boiling：By the glasswort suspension boiling 1.5h after immersion, then decline in air-proof condition and warm to room temperature；
(3) separate：Cooking liquor is carried out into separation of solid and liquid, glasswort cane powder is obtained, the water after separation is carried out into step (1)
Recycle；
(4) become thoroughly decomposed naturally：Control glasswort cane powder water content 40~50%, 30~50 DEG C of temperature control, become thoroughly decomposed 40d naturally；
(5) two-step fermentation enzymolysis：Naturally become thoroughly decomposed after end, add glasswort mass ratio 2% cellulase and 1% it is non-
Starch-polysaccharides enzyme, and the salt tolerant Alkaliphilic bacillus of glasswort mass ratio 2.5% are inoculated with, ferment 4d under the conditions of 28~35 DEG C,
Stirring in 12 hours is once；Glasswort yeast powder is obtained final product after fermentation ends；
(6) co-grinding：By each raw material press extra large 40 parts of Salicornia Bigelovii Torr. Powder, 18 parts of wilsonii, 10 parts of rhodiola root, 10 parts of Gorgon fruit,
Old 4 parts of 7 parts of Divine Comedy, bacterium, 7 parts of balloonflower root, 7 parts of Radix Glycyrrhizae, 4 parts of radix scrophulariae, 6 parts of fructus amomi, 4 parts of ratio mixing of eupatorium, then crushed 100
Mesh sieve；Add the pure water of 3 times of material quality；
(7) digest：Cellulase, 2.5% non-starch polysaccharide enzyme of herbal raw material mass ratio 4% are added, 40~
Temperature control enzymolysis 6h under the conditions of 60 DEG C, every 0.5h stirrings once；
(8) it is separated by filtration：Filtering enzymolysis liquid, obtains enzymolysis liquid and enzymolysis residue；
(9) Ultrasonic Wave-Assisted Extraction：To the pure water that 4 times are added in enzymolysis residue, under ultrasonic assistant effect, 100
Flow back extraction 25min, ultrasonic power 400w, frequency 25kHz under the conditions of DEG C；Ultrasonic power 400w, frequency 25kHz；
(10) it is separated by filtration：Filtering leaching liquor, obtains leaching liquor and leaching residue；
(11) prepared by zymotic fluid：To 5 times of pure water of mass ratio of addition in the leaching residue in step (10), and add residual
The glucose of slag mass ratio 4.0%, 2.5% yeast extract, 0.4% potassium dihydrogen phosphate, 0.02%MgSO4, 0.01%CaCl2, pH is adjusted to
7.0~7.2；
(12) aerobic fermentation：From liquid fermentation tank, liquid amount volume ratio is 60~70%, is inoculated with the sugared bacillus of cold solution
And aspergillus niger, inoculum concentration is respectively 3%, 1.5%, controls 28~37 DEG C of fermentation temperature, 150~300rpm of rotating speed；Ferment herein
Under the conditions of ferment 18h；
(13) anaerobic fermentation：After aerobic fermentation terminates, streptococcus thermophilus and Bacillus acidi lactici are inoculated with, inoculum concentration is 3%, hair
28~37 DEG C of ferment temperature, temperature control anaerobic fermentation 18h；
(14) merge：Enzymolysis liquid in step (8), the zymotic fluid in leaching liquor and step (13) in step (10) are closed
And；
(15) low temperature concentration：Enhancing agent solution after step (14) is merged under cryogenic is concentrated；
(16) it is adsorbed by drying：Adsorbed with 1~1.5 times of wheat bran of mass ratio after concentration, in 40~60 DEG C of blast driers
Dry, moisture is controlled 8~10%；
(17) crush：Dried product is sieved with the mesh of pulverizer 60 and is crushed, obtain final product aquatic immune reinforcing agent.
Embodiment 2：
A kind of snakehead immunopotentiator based on glasswort recycling, specific preparation process is as follows：
(1) immersion is crushed：Deimpurity glasswort cane will be gone to crush, cross 100 mesh sieves, be subsequently adding 2 times of mass ratioes pure
Water purification soaks 5h；
(2) boiling：By the glasswort suspension boiling 2h after immersion, then decline in air-proof condition and warm to room temperature；
(3) separate：Cooking liquor is carried out into separation of solid and liquid, glasswort cane powder is obtained, the water after separation is carried out into step (1)
Recycle；
(4) become thoroughly decomposed naturally：Control glasswort cane powder water content 40~50%, 30~50 DEG C of temperature control, become thoroughly decomposed 30d naturally；
(5) two-step fermentation enzymolysis：Naturally become thoroughly decomposed after end, add glasswort mass ratio 2% cellulase and 1% it is non-
Starch-polysaccharides enzyme, and the salt tolerant Alkaliphilic bacillus of glasswort mass ratio 3% are inoculated with, ferment 5d, 12 under the conditions of 28~35 DEG C
Hour stirring is once；Glasswort yeast powder is obtained final product after fermentation ends；
(6) co-grinding：Each raw material is pressed into 50 parts of Salicornia Bigelovii Torr. Powder, 15 parts of wilsonii, 12 parts of rhodiola root, 8 parts of Gorgon fruit, Divine Comedy
8 parts, old 3 parts of bacterium, 8 parts of balloonflower root, 6 parts of Radix Glycyrrhizae, 5 parts of radix scrophulariae, 5 parts of fructus amomi, the mixing of 5 parts of ratios of eupatorium, then crushed 100 mesh
Sieve；Add the pure water of 3 times of material quality；
(7) digest：Cellulase, 3% non-starch polysaccharide enzyme of herbal raw material mass ratio 5% are added, 40~60
Temperature control enzymolysis 4h under the conditions of DEG C, every 0.5h stirrings once；
(8) it is separated by filtration：Filtering enzymolysis liquid, obtains enzymolysis liquid and enzymolysis residue；
(9) Ultrasonic Wave-Assisted Extraction：To the pure water that 5 times are added in enzymolysis residue, under ultrasonic assistant effect, 100
Flow back extraction 20min, ultrasonic power 400w, frequency 25kHz under the conditions of DEG C；Ultrasonic power 400w, frequency 25kHz；
(10) it is separated by filtration：Filtering leaching liquor, obtains leaching liquor and leaching residue；
(11) prepared by zymotic fluid：To 5 times of pure water of mass ratio of addition in the leaching residue in step (10), and add residual
The glucose of slag mass ratio 5.0%, 2.0% yeast extract, 0.5% potassium dihydrogen phosphate, 0.02%MgSO4, 0.01%CaCl2, pH is adjusted to
7.0~7.2；
(12) aerobic fermentation：From liquid fermentation tank, liquid amount volume ratio is 60~70%, is inoculated with the sugared bacillus of cold solution
And aspergillus niger, inoculum concentration is respectively 4%, 2%, controls 28~37 DEG C of fermentation temperature, 150~300rpm of rotating speed；Ferment bar herein
Ferment 12h under part；
(13) anaerobic fermentation：After aerobic fermentation terminates, streptococcus thermophilus and Bacillus acidi lactici are inoculated with, inoculum concentration is 4%, hair
28~37 DEG C of ferment temperature, temperature control anaerobic fermentation 12h；
(14) merge：Enzymolysis liquid in step (8), the zymotic fluid in leaching liquor and step (13) in step (10) are closed
And；
(15) low temperature concentration：Enhancing agent solution after step (14) is merged under cryogenic is concentrated；
(16) it is adsorbed by drying：Adsorbed with 1~1.5 times of wheat bran of mass ratio after concentration, in 40~60 DEG C of blast driers
Dry, moisture is controlled 8~10%；
(17) crush：Dried product is sieved with the mesh of pulverizer 60 and is crushed, obtain final product aquatic immune reinforcing agent.
Embodiment 3：
A kind of carp immunostimulant for fish based on glasswort recycling, specific preparation process is as follows：
(1) immersion is crushed：Deimpurity glasswort cane will be gone to crush, cross 100 mesh sieves, be subsequently adding 2 times of mass ratioes pure
Water purification soaks 3h；
(2) boiling：By the glasswort suspension boiling 1h after immersion, then decline in air-proof condition and warm to room temperature；
(3) separate：Cooking liquor is carried out into separation of solid and liquid, glasswort cane powder is obtained, the water after separation is carried out into step (1)
Recycle；
(4) become thoroughly decomposed naturally：Control glasswort cane powder water content 40~50%, 30~50 DEG C of temperature control, become thoroughly decomposed 50d naturally；
(5) two-step fermentation enzymolysis：Naturally become thoroughly decomposed after end, add glasswort mass ratio 2% cellulase and 1% it is non-
Starch-polysaccharides enzyme, and the salt tolerant Alkaliphilic bacillus of glasswort mass ratio 2% are inoculated with, ferment 5d, 12 under the conditions of 28~35 DEG C
Hour stirring is once；Glasswort yeast powder is obtained final product after fermentation ends；
(6) co-grinding：Each raw material is pressed into 30 parts of Salicornia Bigelovii Torr. Powder, 20 parts of wilsonii, 8 parts of rhodiola root, 12 parts of Gorgon fruit, Divine Comedy
6 parts, old 5 parts of bacterium, 6 parts of balloonflower root, 8 parts of Radix Glycyrrhizae, 3 parts of radix scrophulariae, 7 parts of fructus amomi, the mixing of 3 parts of ratios of eupatorium, then crushed 100 mesh
Sieve；Add the pure water of 3 times of material quality；
(7) digest：Cellulase, 2% non-starch polysaccharide enzyme of herbal raw material mass ratio 3% are added, 40~60
Temperature control enzymolysis 8h under the conditions of DEG C, every 0.5h stirrings once；
(8) it is separated by filtration：Filtering enzymolysis liquid, obtains enzymolysis liquid and enzymolysis residue；
(9) Ultrasonic Wave-Assisted Extraction：To the pure water that 3 times are added in enzymolysis residue, under ultrasonic assistant effect, 100
Flow back extraction 30min, ultrasonic power 400w, frequency 25kHz under the conditions of DEG C；Ultrasonic power 400w, frequency 25kHz；
(10) it is separated by filtration：Filtering leaching liquor, obtains leaching liquor and leaching residue；
(11) prepared by zymotic fluid：To 5 times of pure water of mass ratio of addition in the leaching residue in step (10), and add residual
The glucose of slag mass ratio 3.0%, 3.0% yeast extract, 0.3% potassium dihydrogen phosphate, 0.02%MgSO4, 0.01%CaCl2, pH is adjusted to
7.0~7.2；
(12) aerobic fermentation：From liquid fermentation tank, liquid amount volume ratio is 60~70%, is inoculated with the sugared bacillus of cold solution
And aspergillus niger, inoculum concentration is respectively 2%, 1%, controls 28~37 DEG C of fermentation temperature, 150~300rpm of rotating speed；Ferment bar herein
Ferment 24h under part；
(13) anaerobic fermentation：After aerobic fermentation terminates, streptococcus thermophilus and Bacillus acidi lactici are inoculated with, inoculum concentration is 2%, hair
28~37 DEG C of ferment temperature, temperature control anaerobic fermentation 24h；
(14) merge：Enzymolysis liquid in step (8), the zymotic fluid in leaching liquor and step (13) in step (01) are closed
And；
(15) low temperature concentration：Enhancing agent solution after step (14) is merged under cryogenic is concentrated；
(16) it is adsorbed by drying：Adsorbed with 1~1.5 times of wheat bran of mass ratio after concentration, in 40~60 DEG C of blast driers
Dry, moisture is controlled 8~10%；
(17) crush：Dried product is sieved with the mesh of pulverizer 60 and is crushed, obtain final product aquatic immune reinforcing agent.
The technique effect that the application is obtained is illustrated with checking test below：
Table 1 tests grouping sheet
The result of the test of table 2
Above-mentioned result of the test shows that the application immunopotentiator achieves significant technique effect, more existing commercially available medium-height grass
Medicine immunopotentiator significant difference (P<0.01), and result of the test show feed change composition can significantly reduce product effect (P<
0.01)。
Above example is merely to illustrate technical scheme, rather than is limited；Although with reference to foregoing reality
Example is applied to being described in detail by invention, but for the person of ordinary skill of the art, still can be to foregoing reality
Apply the technical scheme described in example to modify, or equivalent is carried out to which part technical characteristic；And to these modifications
Or replace, do not make the spirit and scope of the essence disengaging claimed technical solution of the invention of appropriate technical solution.
Claims (4)
1. a kind of aquatic immune reinforcing agent based on glasswort recycling, it is characterised in that by the raw material system of following mass ratio
Into：30~50 parts of Salicornia Bigelovii Torr. Powder, 15~20 parts of wilsonii, 8~12 parts of rhodiola root, 8~12 parts of Gorgon fruit, 6~8 parts of Divine Comedy, bacterium old 3
~5 parts, 6~8 parts of balloonflower root, 6~8 parts of Radix Glycyrrhizae, 3~5 parts of radix scrophulariae, 5~7 parts of fructus amomi, 3~5 parts of eupatorium.
2. a kind of aquatic immune reinforcing agent based on glasswort recycling according to claim 1, it is characterised in that
The raw material composition is preferably：40 parts of Salicornia Bigelovii Torr. Powder, 18 parts of wilsonii, 10 parts of rhodiola root, 10 parts of Gorgon fruit, 7 parts of Divine Comedy, bacterium old 4
Part, 7 parts of balloonflower root, 7 parts of Radix Glycyrrhizae, 4 parts of radix scrophulariae, 6 parts of fructus amomi, 4 parts of eupatorium.
3. a kind of aquatic immune reinforcing agent based on glasswort recycling according to claim 1, it is characterised in that
The Salicornia Bigelovii Torr. Powder pretreating process is as follows：
（1）Crush immersion：Deimpurity glasswort cane will be gone to crush, cross 100 mesh sieves, and be subsequently adding 2 times of quality and compare pure water
3~5h of immersion；
（2）Boiling：By the glasswort suspension 1~2h of boiling after immersion, then decline in air-proof condition and warm to room temperature；
（3）Separate：Cooking liquor is carried out into separation of solid and liquid, glasswort cane powder is obtained, the water after separation enters step（1）It is circulated
Utilize；
（4）Naturally become thoroughly decomposed：Control glasswort cane powder water content 40~50%, 30~50 DEG C of temperature control, become thoroughly decomposed 30~50d naturally；
（5）Two-step fermentation is digested：Naturally become thoroughly decomposed after end, add the cellulase and 1% non-starch of glasswort mass ratio 2% many
Carbohydrase, and the salt tolerant Alkaliphilic bacillus of glasswort mass ratio 2~3% are inoculated with, ferment 3~5d under the conditions of 28~35 DEG C, and 12 is small
When stirring once；Glasswort yeast powder is obtained final product after fermentation ends.
4. a kind of aquatic immune reinforcing agent based on glasswort recycling according to claim 1, it is characterised in that
The immunopotentiator is made by the steps：
（1）Co-grinding：Each raw material is mixed in proportion, 100 mesh sieves were then crushed；Add the pure water of 3 times of material quality；
（2）Enzymolysis：Cellulase, 2~3% non-starch polysaccharide enzyme of herbal raw material mass ratio 3~5% are added, 40~60
Temperature control digests 4~8h under the conditions of DEG C, every 0.5h stirrings once；
（3）It is separated by filtration：Filtering enzymolysis liquid, obtains enzymolysis liquid and enzymolysis residue；
（4）Ultrasonic Wave-Assisted Extraction：To the pure water that 3~5 times are added in enzymolysis residue, under ultrasonic assistant effect, 100 DEG C
Under the conditions of flow back extraction 20~30min, ultrasonic power 400w, frequency 25kHz；Ultrasonic power 400w, frequency 25kHz；
（5）It is separated by filtration：Filtering leaching liquor, obtains leaching liquor and leaching residue；
（6）It is prepared by zymotic fluid：To step（5）In leaching residue in add 5 times of pure water of mass ratio, and add mass of residue
Than 3.0~5.0% glucose, 2.0~3.0% yeast extracts, 0.3~0.5% potassium dihydrogen phosphate, 0.02%MgSO4、0.01%CaCl2, pH
It is adjusted to 7.0~7.2；
（7）Aerobic fermentation：From liquid fermentation tank, liquid amount volume ratio is 60~70%, is inoculated with the cold sugared bacillus of solution and black song
Mould, inoculum concentration is respectively 2~4%, 1~2%, controls 28~37 DEG C of fermentation temperature, 150~300rpm of rotating speed；In this fermentation condition
12~24h of lower fermentation；
（8）Anaerobic fermentation：After aerobic fermentation terminates, streptococcus thermophilus and Bacillus acidi lactici are inoculated with, inoculum concentration is 2~4%, fermentation
28~37 DEG C of temperature, 12~24h of temperature control anaerobic fermentation；
（9）Merge：By step（3）In enzymolysis liquid, step（5）In leaching liquor and step（8）In zymotic fluid merge；
（10）Low temperature is concentrated：Under cryogenic by step（9）Enhancing agent solution after merging is concentrated；
（11）It is adsorbed by drying：Adsorbed with 1~1.5 times of wheat bran of mass ratio after concentration, dried in 40~60 DEG C of blast driers,
Moisture is controlled 8~10%；
（12）Crush：Dried product is sieved with the mesh of pulverizer 60 and is crushed, obtain final product aquatic immune reinforcing agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611239681.2A CN106689818A (en) | 2016-12-28 | 2016-12-28 | Aquatic product immunopotentiator based on salicornia bigelovii torr resource utilization |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611239681.2A CN106689818A (en) | 2016-12-28 | 2016-12-28 | Aquatic product immunopotentiator based on salicornia bigelovii torr resource utilization |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106689818A true CN106689818A (en) | 2017-05-24 |
Family
ID=58902840
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611239681.2A Pending CN106689818A (en) | 2016-12-28 | 2016-12-28 | Aquatic product immunopotentiator based on salicornia bigelovii torr resource utilization |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106689818A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107549514A (en) * | 2017-10-23 | 2018-01-09 | 无为县雨露生态农业有限公司 | Fresh-water fishes ecologic breeding feed and preparation method thereof |
| CN107568506A (en) * | 2017-10-23 | 2018-01-12 | 无为县雨露生态农业有限公司 | Fresh water fish feed and preparation method thereof |
| CN107581408A (en) * | 2017-10-23 | 2018-01-16 | 无为县雨露生态农业有限公司 | Mixed composite microbial feed for freshwater fish and preparation method thereof |
| CN110024968A (en) * | 2019-06-05 | 2019-07-19 | 文大鹏 | A kind of prepared food material packet and application method |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103583928A (en) * | 2013-12-02 | 2014-02-19 | 郝智慧 | Feed used for improving immune functions of grass carp |
| CN103609848A (en) * | 2013-11-26 | 2014-03-05 | 青岛嘉瑞生物技术有限公司 | Fermented Chinese herbal medicine feed additive for aquatic products and preparation method thereof |
| CN103933509A (en) * | 2014-04-02 | 2014-07-23 | 李淑翠 | Medicine for treating saprolegniasis of freshwater fish and preparation method of medicine |
| CN104026377A (en) * | 2014-05-16 | 2014-09-10 | 安徽蓝博旺现代农业科技有限公司 | Soft-shelled turtle feed containing egg yolk and fish oil |
| CN104054972A (en) * | 2014-07-08 | 2014-09-24 | 明光市永言水产食品有限公司 | Feed for raising river crab |
| CN104082631A (en) * | 2014-07-11 | 2014-10-08 | 郝智慧 | Feed and traditional Chinese medicine composition for preventing and curing white-body diseases of macrobrachium rosenbergii and preparation method |
| CN104186970A (en) * | 2014-07-30 | 2014-12-10 | 严中明 | Fish feed for preventing and treating hemorrhagic diseases of grass carps |
| CN104222674A (en) * | 2014-09-30 | 2014-12-24 | 青岛嘉瑞生物技术有限公司 | Preparation method and application of Chinese herbal medicine immunoenhancer used for channel catfish |
| CN105746845A (en) * | 2014-12-15 | 2016-07-13 | 陆红梅 | Health freshwater fish composite feed |
| CN106071405A (en) * | 2016-06-27 | 2016-11-09 | 青岛铭晨信息技术有限公司 | A kind of preparation method and application of Penaeus vannamei Chinese medicine immunity enhancer |
-
2016
- 2016-12-28 CN CN201611239681.2A patent/CN106689818A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609848A (en) * | 2013-11-26 | 2014-03-05 | 青岛嘉瑞生物技术有限公司 | Fermented Chinese herbal medicine feed additive for aquatic products and preparation method thereof |
| CN103583928A (en) * | 2013-12-02 | 2014-02-19 | 郝智慧 | Feed used for improving immune functions of grass carp |
| CN103933509A (en) * | 2014-04-02 | 2014-07-23 | 李淑翠 | Medicine for treating saprolegniasis of freshwater fish and preparation method of medicine |
| CN104026377A (en) * | 2014-05-16 | 2014-09-10 | 安徽蓝博旺现代农业科技有限公司 | Soft-shelled turtle feed containing egg yolk and fish oil |
| CN104054972A (en) * | 2014-07-08 | 2014-09-24 | 明光市永言水产食品有限公司 | Feed for raising river crab |
| CN104082631A (en) * | 2014-07-11 | 2014-10-08 | 郝智慧 | Feed and traditional Chinese medicine composition for preventing and curing white-body diseases of macrobrachium rosenbergii and preparation method |
| CN104186970A (en) * | 2014-07-30 | 2014-12-10 | 严中明 | Fish feed for preventing and treating hemorrhagic diseases of grass carps |
| CN104222674A (en) * | 2014-09-30 | 2014-12-24 | 青岛嘉瑞生物技术有限公司 | Preparation method and application of Chinese herbal medicine immunoenhancer used for channel catfish |
| CN105746845A (en) * | 2014-12-15 | 2016-07-13 | 陆红梅 | Health freshwater fish composite feed |
| CN106071405A (en) * | 2016-06-27 | 2016-11-09 | 青岛铭晨信息技术有限公司 | A kind of preparation method and application of Penaeus vannamei Chinese medicine immunity enhancer |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107549514A (en) * | 2017-10-23 | 2018-01-09 | 无为县雨露生态农业有限公司 | Fresh-water fishes ecologic breeding feed and preparation method thereof |
| CN107568506A (en) * | 2017-10-23 | 2018-01-12 | 无为县雨露生态农业有限公司 | Fresh water fish feed and preparation method thereof |
| CN107581408A (en) * | 2017-10-23 | 2018-01-16 | 无为县雨露生态农业有限公司 | Mixed composite microbial feed for freshwater fish and preparation method thereof |
| CN110024968A (en) * | 2019-06-05 | 2019-07-19 | 文大鹏 | A kind of prepared food material packet and application method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103431174B (en) | Preparation method and application of rice hull/straw feed additive | |
| CN104222674B (en) | The preparation method of a kind of channel catfish Chinese medicine immunity enhancer and application | |
| CN105724772A (en) | Chinese herbal medicine micro-ecological compound feed additive and preparation and use method thereof | |
| CN103621825B (en) | Preparation method and application of fermented Chinese herbal medicine immune enhancer for yellow-head catfish meat | |
| CN103598485A (en) | Preparation method and application of Chinese herbal immunopotentiator used for snakeheads | |
| CN103667220B (en) | Neutral protease-containing growing pig dedicated enzyme and preparation method thereof | |
| CN106689818A (en) | Aquatic product immunopotentiator based on salicornia bigelovii torr resource utilization | |
| CN106306462A (en) | Probiotic fermented feed additive for pigs | |
| CN106071405A (en) | A kind of preparation method and application of Penaeus vannamei Chinese medicine immunity enhancer | |
| CN104187436A (en) | Preparation method of meat rabbit feed additive obtained through fermentation of seaweed and Chinese herbal medicines | |
| CN103766611B (en) | Neutral protease-containing coarse grain daily ration enzyme and preparation method thereof | |
| CN106260504A (en) | A kind of method utilizing beer yeast slurry to produce fermentable moist forage | |
| CN103609858A (en) | Feed additive and preparation method thereof | |
| CN106954731A (en) | A kind of preparation method and applications of bagasse bioactive feed raw material | |
| CN108576424A (en) | Improve the yak fermented feed of growth performance | |
| CN104082528B (en) | A kind of feed addictive and preparation method thereof | |
| CN104472868A (en) | Processing method of biologic feed additive | |
| CN107960548A (en) | Digestant pannage | |
| CN106689817A (en) | Speckled trout puffing mixed feed for resource utilization of salicornia europaea | |
| CN107927366A (en) | New disease-resistant lamb supplement material of height and preparation method thereof | |
| CN106721664A (en) | The technique that a kind of utilization glasswort prepares channel catfish immunopotentiator | |
| CN106616051A (en) | Erythroculter ilishaeformis feed additive added with salicornia bigelovii and enteromorpha | |
| CN103740683B (en) | A kind of Wheat ration enzyme containing neutral protease and preparation method thereof | |
| CN106616050A (en) | Biological method for preparing garrupa immunopotentiator from froggrass | |
| CN104938862A (en) | Breeding sheep Chinese herbal medicine fermentation immunopotentiator preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170524 |