CN103609848A - Fermented Chinese herbal medicine feed additive for aquatic products and preparation method thereof - Google Patents
Fermented Chinese herbal medicine feed additive for aquatic products and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a fermented Chinese herbal medicine feed additive for aquatic products. The fermented Chinese herbal medicine feed additive for aquatic products has various functions of prevention, health-care, treatment, immunoregulation and the like, and is an ideal, green, environment-friendly and safe novel feed additive which can replace antibiotic medicines. The fermented Chinese herbal medicine feed additive is prepared from compound Chinese herbal medicines through a process of Chinese herbal medicine ultrasonic-assisted extraction and multi-strain two-step fermentation, and has double advantages of Chinese herbal medicines and probiotics, so that the additive can efficiently regulate the intestinal health of aquatic animals, treat both symptoms and root causes, consolidate basis, maintain the microecological balance and optimal immune state of the intestinal tract, improve the digestibility of nutrient substances, the immunity of animal bodies and the breeding income, reduce the breeding risk and replace antibiotics, is free from harmful effect to aquatic animals, safe to use and free from problems of medicine residue and tolerance, and has a wide market prospect.
Description
Technical field
The present invention relates to the preparation method of fermentation of Chinese herbal medicine feed addictive for a kind of aquatic products, belong to feed additive field.
Background technology
Aquatic products more and more occupy important share in people's diet, in order to meet the demand of ever-increasing aquatic products, solution becomes the Important Problems of aquaculture because the physiological function that aquaculture scale constantly expands and growth cycle reduction causes is disorderly, disease takes place frequently, and is also the key of aquatic feeds industry development.Under this background, antibiotics feed addictive is subject to heat and holds in both hands, and is widely used in feed industry, has promoted to a certain extent the development of culture fishery, but more serious of the problems such as the high residue that antibiotic brings, high pollution and the resistance to the action of a drug.Current social, food security Frequent Accidents, people also more focus on healthy diet in satisfied having enough to eat and wear, and the aquatic products of green health receive much concern.Chinese herbal feed additive meets the requirement of green aquatic products aquaculture development, has eliminated the safety worries of people to its aquatic products product of feeding out, should vigorously promote the use.
But the research level of current Chinese herbal feed additive is lower, manufacture craft simple coarse, the technological element of a product is lower, and quality is stable not, and problem like that is seriously restricting applying of Chinese medicine fodder interpolation.Microorganism fermented tcm is to the reform of traditional Chinese medicine process technology and innovation, has various features and advantage.This technology is introduced to the research of Chinese herbal feed additive, can make its scientific and technological content significantly promote, effect is more remarkable, more stable quality, benefit is more obvious, can significantly improve immunity of organisms, strengthen the level of aquatic livestock resist the disease, for the various problems that solves aquatic livestock in current culture fishery, have important using value.Utilize microbial fermentation technology to process Chinese herbal medicine, can make the content of its main active substances polysaccharide significantly improve, this seems particularly important for the culture fishery of focusing on aquaculture cost.
Summary of the invention
The object of the invention is to according to above shortcomings in prior art, antibacterial, antiviral, the left and right of improving immunity of organisms and improving growth of animal performance of considering that Chinese herbal medicine has, can reduce a large amount of aquacultures that cause of using of antibiotic pollutes, and raw material sources are wide, cheap, be a kind of green, environmental protection, efficient feed addictive, develop a kind of aquatic products fermentation of Chinese herbal medicine feed addictive.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
An aquatic products fermentation of Chinese herbal medicine feed addictive, is characterized in that compound Chinese herb is comprised of the Chinese herbal medicine of following mass ratio: 15～25 parts of the Radixs Astragali, 10～20 parts of Huang Ling, 10～14 parts of Radix Codonopsis, 6～8 parts of reticulate millettias, 3～7 parts of honeysuckles, 6～10 parts of glossy ganodermas, 3～5 parts of rheum officinales, 6～10 parts of pseudo-ginseng, 8～12 parts of gynostemma pentaphylla, 5～7 parts of Semen sesami nigrums, 4～6 parts, Radix Glycyrrhizae.
The invention provides a kind of aquatic products fermentation of Chinese herbal medicine feed addictive, selected leavening is by bacillus subtilis (Chinese agriculture microorganism fungus kind preservation administrative center, bacterium numbering ACCC10619), S. cervisiae (Angel Yeast), lactobacillus bulgaricus (Chinese agriculture microorganism fungus kind preservation administrative center, bacterium numbering ACCC10638) and bifidobacterium bifidum (ATCC standard bacterial classification, bacterium numbering ATCC29521) composite ferment forming, bacillus subtilis wherein, lactobacillus bulgaricus, the inoculum concentration of bifidobacterium bifidum is 2～4%, S. cervisiae inoculum concentration is 5～8%.
The invention provides the preparation method of fermentation of Chinese herbal medicine feed addictive for a kind of aquatic products, specifically comprise the following steps:
(1) preparation of compound Chinese herb: accurately weigh Chinese herbal medicine according to above-mentioned formula, be crushed to 40～60 orders, mix, make compound Chinese herb;
(2) Ultrasonic Wave-Assisted Extraction: to the water that adds 5～10 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 ℃ of conditions;
(3) zymotic fluid preparation: add 3.0～5.0% soluble starches, 2.0～3.0% beancake powders, 0.3～0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(4) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 2～4%, 5～8%, 28～37 ℃ of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 12～24h;
(5) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is 2～4%, 28～37 ℃ of fermentation temperatures, temperature control anaerobic fermentation 12～24h;
(6) fermentation post processing: after having fermented, fermented liquid is adsorbed with 1.2～2 volume ratio wheat brans, dry in 40～60 ℃ of blast driers, moisture is controlled at 8～10%;
(7) pulverize: by the pulverizer 60 orders pulverizing of sieving for dried product, obtain aquatic products fermentation of Chinese herbal medicine feed addictive.
The invention provides a kind of aquatic products fermentation of Chinese herbal medicine feed addictive, have prevention, health care, treatment and regulate the multiple efficacies such as immunity, is ideal, green, environmental protection, safety, the novel fodder additive of substitute antibiotics medicine.This invention fermentation of Chinese herbal medicine feed addictive one is efficiently to nurse one's health aquatic products animal intestinal health, treats both principal and secondary aspect of disease, and reinvigoration, keeps intestinal microecology balance and best immune state, improves the digestibility of nutriment, improves animal body immunity; The 2nd, can suppress and stop the generation of the harmful bacterias such as cause of disease coliform, clostridium, salmonella, increase beneficial bacteria of intestinal tract, maintain healthy gut flora; The 3rd, strengthen immunization, by stimulating immunity in enteron aisle, be cell, increase the formation of local antibody, increase macrophage activity; The 4th, fermentation of Chinese herbal medicine feed addictive enters in intestines and resists harmful bacteria, reduces stench and harmful components and forms, and makes the ight soil of discharging eliminate stench, in protection breeding environment, improves aquatic products quality.
The invention provides a kind of aquatic products fermentation of Chinese herbal medicine feed addictive, the mass ratio that described aquatic products is used in aquatic feeds complete diet pellet with fermentation of Chinese herbal medicine feedstuff additive product is 0.1～0.5: or described feed addictive premix, concentrate feed in complete diet pellet required ratio add and use.
The invention provides a kind of aquatic products fermentation of Chinese herbal medicine feed addictive, it is advantageous that: the one, in fermentation of Chinese herbal medicine feed addictive preparation process, adopted the production technology of " Chinese herbal medicine Ultrasonic Wave-Assisted Extraction+many bacterial classifications two-step fermentation ", to compare action effect more remarkable with existing Chinese herbal feed additive product; The 2nd, fermentation of Chinese herbal medicine feed addictive has the two-fold advantage of Chinese herbal medicine and probio, can efficiently nurse one's health aquatic products animal intestinal healthy, treat both principal and secondary aspect of disease, reinvigoration, keep intestinal microecology balance and best immune state, improve the digestibility of nutriment, improve animal body immunity, improve cultivation income, reduce cultivation risk; The 3rd, the alternative antibiotic of this product is used, and aquatic livestock is had no adverse effects, and uses safety, does not have the problems such as medicament residue and drug resistance, has wide market prospects.
The specific embodiment
embodiment 1:
An aquatic products fermentation of Chinese herbal medicine feed addictive, concrete preparation process is as follows:
(1) weigh: according to following formula, accurately weigh Chinese herbal medicine: 15 parts of the Radixs Astragali, 20 parts of Huang Ling, 10 parts of Radix Codonopsis, 8 parts of reticulate millettias, 3 parts of honeysuckles, 10 parts of glossy ganodermas, 3 parts of rheum officinales, 10 parts of pseudo-ginseng, 8 parts of gynostemma pentaphylla, 7 parts of Semen sesami nigrums, 4 parts, Radix Glycyrrhizae;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40～60 orders, mixes, make compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: to the water that adds 5 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 ℃ of conditions;
(4) zymotic fluid preparation: add 3.0% soluble starch, 3.0% beancake powder, 0.3% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(5) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 2%, 8%, 28～37 ℃ of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 12h;
(6) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is respectively 2%, 4%, 28～37 ℃ of fermentation temperatures, temperature control anaerobic fermentation 24h;
(7) fermentation post processing: after having fermented, fermented liquid is adsorbed with 1.2 times of volume ratio wheat brans, dry in 40～60 ℃ of blast driers, moisture is controlled at 8～10%;
(8) pulverize: by the pulverizer 60 orders pulverizing of sieving for dried product, obtain aquatic products fermentation of Chinese herbal medicine feed addictive.
embodiment 2:
An aquatic products fermentation of Chinese herbal medicine feed addictive, concrete preparation process is as follows:
(1) weigh: according to following formula, accurately weigh Chinese herbal medicine: 25 parts of the Radixs Astragali, 10 parts of Huang Ling, 14 parts of Radix Codonopsis, 6 parts of reticulate millettias, 7 parts of honeysuckles, 6 parts of glossy ganodermas, 5 parts of rheum officinales, 6 parts of pseudo-ginseng, 12 parts of gynostemma pentaphylla, 5 parts of Semen sesami nigrums, 6 parts, Radix Glycyrrhizae;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40～60 orders, mixes, make compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: to the water that adds 10 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 ℃ of conditions;
(4) zymotic fluid preparation: add 5.0% soluble starch, 2.0% beancake powder, 0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(5) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 4%, 5%, 28～37 ℃ of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 24h;
(6) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is respectively 4%, 2%, 28～37 ℃ of fermentation temperatures, temperature control anaerobic fermentation 12h;
(7) fermentation post processing: after having fermented, fermented liquid is adsorbed with 2 times of volume ratio wheat brans, dry in 40～60 ℃ of blast driers, moisture is controlled at 8～10%;
(8) pulverize: by the pulverizer 60 orders pulverizing of sieving for dried product, obtain aquatic products fermentation of Chinese herbal medicine feed addictive.
embodiment 3:
An aquatic products fermentation of Chinese herbal medicine feed addictive, concrete preparation process is as follows:
(1) weigh: according to following formula, accurately weigh Chinese herbal medicine: 20 parts of the Radixs Astragali, 15 parts of Huang Ling, 12 parts of Radix Codonopsis, 7 parts of reticulate millettias, 5 parts of honeysuckles, 8 parts of glossy ganodermas, 4 parts of rheum officinales, 8 parts of pseudo-ginseng, 10 parts of gynostemma pentaphylla, 6 parts of Semen sesami nigrums, 5 parts, Radix Glycyrrhizae;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40～60 orders, mixes, make compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: to the water that adds 7.5 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 ℃ of conditions;
(4) zymotic fluid preparation: add 4.0% soluble starch, 2.5% beancake powder, 0.4% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(5) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 3%, 6.5%, 28～37 ℃ of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 18h;
(6) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is 2～4%, 28～37 ℃ of fermentation temperatures, temperature control anaerobic fermentation 18h;
(7) fermentation post processing: after having fermented, fermented liquid is adsorbed with 1.6 times of volume ratio wheat brans, dry in 40～60 ℃ of blast driers, moisture is controlled at 8～10%;
(8) pulverize: by the pulverizer 60 orders pulverizing of sieving for dried product, obtain aquatic products fermentation of Chinese herbal medicine feed addictive.
Above embodiment is only for technical scheme of the present invention is described, but not is limited; Although with reference to previous embodiment, to being had been described in detail by invention, for the person of ordinary skill of the art, the technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And to these modifications or replacement, do not make the spirit and scope of essence disengaging the present invention technical scheme required for protection of appropriate technical solution.
Claims (3)
1. an aquatic products fermentation of Chinese herbal medicine feed addictive, is characterized in that being made by " Chinese herbal medicine Ultrasonic Wave-Assisted Extraction+many bacterial classifications two-step fermentation " technique by compound Chinese herb.
2. according to the compound Chinese herb described in claim 1, it is characterized in that being formed by the Chinese herbal medicine of following mass ratio: 15～25 parts of the Radixs Astragali, 10～20 parts of Huang Ling, 10～14 parts of Radix Codonopsis, 6～8 parts of reticulate millettias, 3～7 parts of honeysuckles, 6～10 parts of glossy ganodermas, 3～5 parts of rheum officinales, 6～10 parts of pseudo-ginseng, 8～12 parts of gynostemma pentaphylla, 5～7 parts of Semen sesami nigrums, 4～6 parts, Radix Glycyrrhizae.
3. " Chinese herbal medicine Ultrasonic Wave-Assisted Extraction+many bacterial classifications two-step fermentation " according to claim 1 technique, it is characterized in that specifically comprising with
Lower step:
(1) preparation of compound Chinese herb: accurately weigh Chinese herbal medicine according to above-mentioned formula, be crushed to 40～60 orders, mix, make compound Chinese herb;
(2) Ultrasonic Wave-Assisted Extraction: to the water that adds 5～10 times in compound Chinese herb pulvis, under ultrasonic wave booster action, lixiviate 20～30min refluxes under 100 ℃ of conditions;
(3) zymotic fluid preparation: add 3.0～5.0% soluble starches, 2.0～3.0% beancake powders, 0.3～0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0～7.2;
(4) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60～70%, inoculation bacillus subtilis and S. cervisiae, inoculum concentration is respectively 2～4%, 5～8%, 28～37 ℃ of controlled fermentation temperature, rotating speed 150～300rpm, throughput 10～20L/min; At this fermentation condition bottom fermentation 12～24h;
(5) anaerobic fermentation: after aerobic fermentation finishes, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is 2～4%, 28～37 ℃ of fermentation temperatures, temperature control anaerobic fermentation 12～24h;
(6) fermentation post processing: after having fermented, fermented liquid is adsorbed with 1.2～2 volume ratio wheat brans, dry in 40～60 ℃ of blast driers, moisture is controlled at 8～10%;
(7) pulverize: by the pulverizer 60 orders pulverizing of sieving for dried product, obtain aquatic products fermentation of Chinese herbal medicine feed addictive.
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