CN104886355A - Compound Chinese herbal medicine feed additive capable of promoting reproduction performance of ram - Google Patents
Compound Chinese herbal medicine feed additive capable of promoting reproduction performance of ram Download PDFInfo
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Abstract
The invention discloses a compound Chinese herbal medicine feed additive capable of promoting reproduction performance of a ram. The compound Chinese herbal medicine feed additive capable of promoting reproduction performance of the ram is prepared through a process of Chinese herbal medicine ultrasonic assisted extraction plus two-step fermentation including multi-strain aerobic fermentation and anaerobic fermentation by using the following raw materials in parts: 12-18 parts of rose root, 8-12 parts of mulberry fruit, 8-12 parts of epimedium herb, 6-10 parts of morinda root, 3-5 parts of dodder seed, 6-10 parts of tuber onion seed, 6-8 parts of liquorice root, 3-5 parts of palmleaf raspberry fruit, 6-10 parts of eucommia bark, 6-10 parts of prepared rehmannia root, 6-10 parts of flatstem milkvetch seed, 5-7 parts of Gynostemma pentaphyllum and 3-5 parts of Chinese wolfberry. The fermented Chinese herbal medicine feed additive disclosed by the invention has the advantages that the sexual desire of the ram can be improved, the sperm generation is promoted, the sperm activity is improved, the reproduction performance of the ram is effectively improved, the scale reproduction benefit is improved, the safety in use is high, the problems such as medicine residue and medicine resistance do not exist, and the market prospect is wide.
Description
Technical field
The present invention relates to a kind of preparation method of Chinese herbal medicine feed additive, a kind of Chinese herbal medicine feed additive that can promote ram reproductive performance, belongs to feed additive field specifically.
Background technology
Along with the transformation of growth in the living standard and consumption structure, mutton because its meat flavour is delicious, the kind of delicate, the contained primary amino acid of fiber and trace element and quantity can meet the needs of human body completely, particularly lamb has that lean meat is many, muscle fat is few, the advantage such as delicious succulence, easily digestion quite gets consumer reception, and is the traditional food and medicament dual-purpose meat product of China.But because Mutton yield is few, price is high, and has smell of mutton, its consumption figure is little compared with the meats such as pork, beef, chicken.In recent years, along with the intensive fast development of animal husbandry, sheep technology of artificial insemination is applied further extensive, but in application sheep technology of artificial insemination is promoted, there are distinct issues, most of sheep is seasonal oestrus, breeding time is more concentrated, makes ram semen collection frequency too high.High strength semen collection, causes ram sexual desire to decline, and ejaculate volume reduces, and semen quality is deteriorated, and ewe non-return rate reduces, and especially summer is even more serious.The utilization periods of ram shortens, and directly affects the Popularizing of A of technology of artificial insemination, although take to adjust trophic level, adds sharp movement, still can not deal with problems completely.
Feed safety is source and the guarantee of animal food safety, and the Forming Mechanism of animal product quality and regulation and control are one of international new problems, and therefore safety in utilization feed addictive, utilizes nutrition regulation method to improve cultivation scale more and more urgent.Chinese herb feed additive has no side effect with it, without hazard residue, the advantage such as have no drug resistance, and meets the requirement of green non-pollution aquaculture development, receives the concern of domestic and international experts and scholars gradually.But the research level of current Chinese herbal feed additive is lower, manufacture craft simple coarse, the technological element of a product is lower, quality is stable not, problem like this seriously governs applying of Chinese medicine fodder interpolation, as patent CN101623057A discloses a kind of Chinese herbal medicine feed additive for stud rams, employing be pulverize after mixing simple preparation technology.Fermentable Chinese medicine is reform to traditional Chinese medicine process technology and innovation, has various features and advantage.This technology is introduced the research of Chinese herbal feed additive, its scientific and technological content can be made significantly to promote, effect is more remarkable, quality is more stable, benefit is more obvious, can significantly improve immunity of organisms, strengthens the level of resist the disease, improve ram reproductive performance, have important using value for the various disadvantages solved in current cultivation industry.Utilize microbial fermentation technology to process Chinese herbal medicine, the content of its major function active material can be made to significantly improve, this seems particularly important for the aquaculture focusing on aquaculture cost, and contribute to the carcass quality significantly promoting cultivation body, meeting the dual interests of cultivation and consumer, is direction and the trend of the healthy green non-pollution sustainable development of aquaculture from now on.
Summary of the invention
The object of the invention is to according to above shortcomings in prior art, consider that Chinese herbal medicine has the effect improving buck reproductive performance, use safety, and raw material sources are wide, cheap, be a kind of green, environmental protection, efficiently feed addictive, develop a kind of compound Chinese herbal medicinal feed additive improving ram sexual desire, promote Sperm specific enzyme, improve sperm motility, effectively improve ram reproductive performance.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
Promote a compound Chinese herbal medicinal feed additive for ram reproductive performance, it is characterized in that compound Chinese herb is made up of the Chinese herbal medicine of following mass ratio: rhodiola root 12 ~ 18 parts, 8 ~ 12 parts, mulberry fruit, barrenwort 8 ~ 12 parts, Morinda officinalis 6 ~ 10 parts, the seed of Chinese dodder 3 ~ 5 parts, semen allii tuberosi 6 ~ 10 parts, 6 ~ 8 parts, Radix Glycyrrhizae, raspberry 3 ~ 5 parts, the bark of eucommia 6 ~ 10 parts, 6 ~ 10 parts, cultivated land, SEMEN ASTRAGALI COMPLANATI 6 ~ 10 parts, gynostemma pentaphylla 5 ~ 7 parts, matrimony vine 3 ~ 5 parts.
The invention provides a kind of compound Chinese herbal medicinal feed additive promoting ram reproductive performance, selected leavening is the composite ferment be made up of bacillus licheniformis, candida utili, bifidobacterium bifidum and Lactobacillus plantarum, and wherein the inoculum concentration of bacillus licheniformis, bifidobacterium bifidum, Lactobacillus plantarum is 2 ~ 4%, candida utili inoculum concentration is 5 ~ 8%.
The invention provides a kind of preparation method promoting the compound Chinese herbal medicinal feed additive of ram reproductive performance, specifically comprise the following steps:
(1) preparation of compound Chinese herb: according to above-mentioned formula precise Chinese herbal medicine, be crushed to 40 ~ 60 orders, mix, obtained compound Chinese herb;
(2) Ultrasonic Wave-Assisted Extraction: the water adding mass ratio 5 ~ 10 times in compound Chinese herb pulvis, under ultrasonic assistant effect, reflux under 100 DEG C of conditions lixiviate 20 ~ 30min, ultrasonic power 400w, frequency 25kHz;
(3) zymotic fluid preparation: add leaching liquor mass ratio 3.0 ~ 5.0% glucose, 2.0 ~ 3.0% yeast extracts, 0.3 ~ 0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(4) aerobic fermentation: select liquid fermentation tank, liquid amount is 60 ~ 70%(volume ratio), inoculation bacillus licheniformis and candida utili, inoculum concentration is respectively 2 ~ 4%, 5 ~ 8%(volume ratio), control fermentation temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, throughput 10 ~ 20L/min; At this fermentation condition bottom fermentation 12 ~ 24h;
(5) anaerobic fermentation: after aerobic fermentation terminates, inoculation bifidobacterium bifidum and Lactobacillus plantarum, inoculum concentration is 2 ~ 4%(volume ratio), fermentation temperature 28 ~ 37 DEG C, temperature control anaerobic fermentation 12 ~ 24h;
(6) fermentation post processing: after having fermented, the wheat bran of fermented liquid by 1.2 ~ 2 times of volume ratios is adsorbed, dry in 40 ~ 60 DEG C of blast driers, moisture controls 8 ~ 10%;
(7) pulverize: pulverizing of being sieved by dried product pulverizer 60 order, obtains Chinese herbal medicine feed additive.
The invention provides a kind of compound Chinese herbal medicinal feed additive promoting ram reproductive performance, having and improve the multiple efficacies such as ram sexual desire, promotion Sperm specific enzyme, raising sperm motility, effectively raising ram reproductive performance, is the ideal of substitute antibiotics medicine, green, environmental protection, safety, novel fodder additive.
The invention provides a kind of compound Chinese herbal medicinal feed additive promoting ram reproductive performance, the mass ratio that described Chinese herbal medicine feed additive product uses in diet feed material is 0.1 ~ 0.5%: or the ratio interpolation use that described feed addictive is required in premix, concentrate feed are with complete diet pellet.
The invention provides a kind of compound Chinese herbal medicinal feed additive promoting ram reproductive performance, it is advantageous that: one is the production technology that have employed " Chinese herbal medicine Ultrasonic Wave-Assisted Extraction+multi-cultur es aerobic fermentation and anaerobic fermentation combine two-step fermentation " in fermentation of Chinese herbal medicine feed addictive preparation process, and action effect is more remarkable compared with existing Chinese herbal feed additive product, two be science composite multiple have consolidate help, the Chinese herbal medicine of the effects such as tonifying kidney and strengthening yang, can effectively improve ram sexual desire, promote Sperm specific enzyme, improve sperm motility, effective raising ram reproductive performance, demonstration test result ram property reaction time after Chinese herbal feed additive 20d of the present invention that shows to feed is adopted to reach 54.6 ± 6.8s, ejaculation mounting number of times reaches 4.25 ± 1.2, ejaculate volume reaches 2.11 ± 0.35ml, sperm concentration reaches 48.69 ± 5.41 hundred million/mL, sperm motility reaches 86.94 ± 6.974%, all be significantly higher than current report level, and rate of teratosperm significantly reduces, total abnormal rate is only 2.1 ± 0.5%, in addition, the result shows the composition or the remarkable action effect (p<0.05) reducing product of the present invention of product preparation process meeting that change Chinese herbal medicine, three is two-fold advantage that fermentation of Chinese herbal medicine feed addictive has Chinese herbal medicine and probio, can treat both principal and secondary aspect of disease, reinvigoration, improves animal body immunity and ram reproductive performance, improves cultivation income, reduces cultivation risk, four is use safety, there is not the problem such as medicament residue and drug resistance, has wide market prospects.
Detailed description of the invention
embodiment 1:
Promote a compound Chinese herbal medicinal feed additive for ram reproductive performance, concrete preparation process is as follows:
(1) weigh: according to following formula precise Chinese herbal medicine: rhodiola root 12 parts, 12 parts, mulberry fruit, barrenwort 8 parts, Morinda officinalis 10 parts, the seed of Chinese dodder 3 parts, semen allii tuberosi 10 parts, 6 parts, Radix Glycyrrhizae, raspberry 5 parts, the bark of eucommia 6 parts, 10 parts, cultivated land, SEMEN ASTRAGALI COMPLANATI 6 parts, gynostemma pentaphylla 7 parts, matrimony vine 3 parts;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40 ~ 60 orders, mixes, obtained compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: the water adding mass ratio 5 times in compound Chinese herb pulvis, under ultrasonic assistant effect, reflux under 100 DEG C of conditions lixiviate 20 ~ 30min, ultrasonic power 400w, frequency 25kHz;
(4) zymotic fluid preparation: add leaching liquor mass ratio 3.0% glucose, 3.0% yeast extract, 0.3% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(5) aerobic fermentation: select liquid fermentation tank, liquid amount is 60 ~ 70%(volume ratio), inoculation bacillus licheniformis and candida utili, inoculum concentration is respectively 2%, 8%(volume ratio), control fermentation temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, throughput 10 ~ 20L/min; At this fermentation condition bottom fermentation 12h;
(6) anaerobic fermentation: after aerobic fermentation terminates, inoculation bifidobacterium bifidum and Lactobacillus plantarum, inoculum concentration is respectively 2%, 4%(volume ratio), fermentation temperature 28 ~ 37 DEG C, temperature control anaerobic fermentation 24h;
(7) fermentation post processing: after having fermented, the wheat bran of fermented liquid by 1.2 times of volume ratios is adsorbed, dry in 40 ~ 60 DEG C of blast driers, moisture controls 8 ~ 10%;
(8) pulverize: pulverizing of being sieved by dried product pulverizer 60 order, obtains Chinese herbal medicine feed additive.
embodiment 2:
Promote a compound Chinese herbal medicinal feed additive for ram reproductive performance, concrete preparation process is as follows:
(1) weigh: according to following formula precise Chinese herbal medicine: rhodiola root 18 parts, 8 parts, mulberry fruit, barrenwort 12 parts, Morinda officinalis 6 parts, the seed of Chinese dodder 5 parts, semen allii tuberosi 6 parts, 8 parts, Radix Glycyrrhizae, raspberry 3 parts, the bark of eucommia 10 parts, 6 parts, cultivated land, SEMEN ASTRAGALI COMPLANATI 10 parts, gynostemma pentaphylla 5 parts, matrimony vine 5 parts;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40 ~ 60 orders, mixes, obtained compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: the water adding mass ratio 10 times in compound Chinese herb pulvis, under ultrasonic assistant effect, reflux under 100 DEG C of conditions lixiviate 20 ~ 30min, ultrasonic power 400w, frequency 25kHz;
(4) zymotic fluid preparation: add leaching liquor mass ratio 5.0% glucose, 2.0% yeast extract, 0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(5) aerobic fermentation: select liquid fermentation tank, liquid amount is 60 ~ 70%(volume ratio), inoculation bacillus licheniformis and candida utili, inoculum concentration is respectively 4%, 5%(volume ratio), control fermentation temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, throughput 10 ~ 20L/min; At this fermentation condition bottom fermentation 24h;
(6) anaerobic fermentation: after aerobic fermentation terminates, inoculation bifidobacterium bifidum and Lactobacillus plantarum, inoculum concentration is respectively 4%, 2%(volume ratio), fermentation temperature 28 ~ 37 DEG C, temperature control anaerobic fermentation 12h;
(7) fermentation post processing: after having fermented, the wheat bran of fermented liquid by 2 times of volume ratios is adsorbed, dry in 40 ~ 60 DEG C of blast driers, moisture controls 8 ~ 10%;
(8) pulverize: pulverizing of being sieved by dried product pulverizer 60 order, obtains Chinese herbal medicine feed additive.
embodiment 3:
Promote a compound Chinese herbal medicinal feed additive for ram reproductive performance, concrete preparation process is as follows:
(1) weigh: according to following formula precise Chinese herbal medicine: rhodiola root 15 parts, 10 parts, mulberry fruit, barrenwort 10 parts, Morinda officinalis 8 parts, the seed of Chinese dodder 4 parts, semen allii tuberosi 8 parts, 7 parts, Radix Glycyrrhizae, raspberry 4 parts, the bark of eucommia 8 parts, 8 parts, cultivated land, SEMEN ASTRAGALI COMPLANATI 8 parts, gynostemma pentaphylla 6 parts, matrimony vine 4 parts;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40 ~ 60 orders, mixes, obtained compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: the water adding mass ratio 7.5 times in compound Chinese herb pulvis, under ultrasonic assistant effect, reflux under 100 DEG C of conditions lixiviate 20 ~ 30min, ultrasonic power 400w, frequency 25kHz;
(4) zymotic fluid preparation: add leaching liquor mass ratio 4.0% glucose, 2.5% yeast extract, 0.4% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(5) aerobic fermentation: select liquid fermentation tank, liquid amount is 60 ~ 70%(volume ratio), inoculation bacillus licheniformis and candida utili, inoculum concentration is respectively 3%, 6.5%(volume ratio), control fermentation temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, throughput 10 ~ 20L/min; At this fermentation condition bottom fermentation 18h;
(6) anaerobic fermentation: after aerobic fermentation terminates, inoculation bifidobacterium bifidum and Lactobacillus plantarum, inoculum concentration is 3%(volume ratio), fermentation temperature 28 ~ 37 DEG C, temperature control anaerobic fermentation 18h;
(7) fermentation post processing: after having fermented, the wheat bran of fermented liquid by 1.6 times of volume ratios is adsorbed, dry in 40 ~ 60 DEG C of blast driers, moisture controls 8 ~ 10%;
(8) pulverize: pulverizing of being sieved by dried product pulverizer 60 order, obtains Chinese herbal medicine feed additive.
Above embodiment only for illustration of technical scheme of the present invention, but not is limited; Although be described in detail by invention with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or equivalent replacement has been carried out to wherein portion of techniques feature; And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (1)
1. promote a compound Chinese herbal medicinal feed additive for ram reproductive performance, it is characterized in that preparation method specifically comprises the following steps:
(1) preparation of compound Chinese herb: according to rhodiola root 12 ~ 18 parts, 8 ~ 12 parts, mulberry fruit, barrenwort 8 ~ 12 parts, Morinda officinalis 6 ~ 10 parts, the seed of Chinese dodder 3 ~ 5 parts, semen allii tuberosi 6 ~ 10 parts, 6 ~ 8 parts, Radix Glycyrrhizae, raspberry 3 ~ 5 parts, the bark of eucommia 6 ~ 10 parts, 6 ~ 10 parts, cultivated land, SEMEN ASTRAGALI COMPLANATI 6 ~ 10 parts, gynostemma pentaphylla 5 ~ 7 parts, matrimony vine 3 ~ 5 parts formula precise Chinese herbal medicine, be crushed to 40 ~ 60 orders, mix, obtained compound Chinese herb;
(2) Ultrasonic Wave-Assisted Extraction: the water adding mass ratio 5 ~ 10 times in compound Chinese herb pulvis, under ultrasonic assistant effect, reflux under 100 DEG C of conditions lixiviate 20 ~ 30min, ultrasonic power 400w, frequency 25kHz;
(3) zymotic fluid preparation: add leaching liquor mass ratio 3.0 ~ 5.0% glucose, 2.0 ~ 3.0% yeast extracts, 0.3 ~ 0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(4) aerobic fermentation: select liquid fermentation tank, liquid amount is 60 ~ 70%, inoculation bacillus licheniformis and candida utili, and inoculum concentration is respectively 2 ~ 4%, 5 ~ 8%, controls fermentation temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, throughput 10 ~ 20L/min; At this fermentation condition bottom fermentation 12 ~ 24h;
(5) anaerobic fermentation: after aerobic fermentation terminates, inoculation bifidobacterium bifidum and Lactobacillus plantarum, inoculum concentration is 2 ~ 4%(volume ratio), fermentation temperature 28 ~ 37 DEG C, temperature control anaerobic fermentation 12 ~ 24h;
(6) fermentation post processing: after having fermented, the wheat bran of fermented liquid by 1.2 ~ 2 times of volume ratios is adsorbed, dry in 40 ~ 60 DEG C of blast driers, moisture controls 8 ~ 10%;
(7) pulverize: pulverizing of being sieved by dried product pulverizer 60 order, obtains Chinese herbal medicine feed additive.
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Cited By (5)
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| CN107668341A (en) * | 2017-08-08 | 2018-02-09 | 上海朝翔生物技术有限公司 | A kind of natural galenical for improving male animal sperm quality and preparation method thereof |
| CN108378197A (en) * | 2018-01-26 | 2018-08-10 | 普安县凯升农业发展有限公司 | One stud ram feed addictive and preparation method thereof |
| CN109601476A (en) * | 2018-12-11 | 2019-04-12 | 中国农业科学院北京畜牧兽医研究所 | A kind of method of novel raising sheep breeding potential |
| CN110537622A (en) * | 2019-08-17 | 2019-12-06 | 广东省农业科学院动物科学研究所 | Fermentation method of mulberry pomace and application thereof |
| CN116103207A (en) * | 2023-03-17 | 2023-05-12 | 山东中科嘉亿生物工程有限公司 | Bifidobacterium bifidum JYBB-163 for improving semen quality and fertility of male, and microbial inoculum, preparation method and application thereof |
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Application publication date: 20150909 |