CN101983582A - Traditional Chinese medicine microecological preparation for preventing and treating enteritis of grass carp and preparation method thereof - Google Patents

Traditional Chinese medicine microecological preparation for preventing and treating enteritis of grass carp and preparation method thereof Download PDF

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Publication number
CN101983582A
CN101983582A CN2010105630625A CN201010563062A CN101983582A CN 101983582 A CN101983582 A CN 101983582A CN 2010105630625 A CN2010105630625 A CN 2010105630625A CN 201010563062 A CN201010563062 A CN 201010563062A CN 101983582 A CN101983582 A CN 101983582A
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chinese medicine
grass carp
feed
bacillus subtilis
traditional chinese
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CN2010105630625A
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Chinese (zh)
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CN101983582B (en
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李旭宁
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广州市海维饲料有限公司
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Abstract

The invention relates to a feed additive and a preparation method thereof, in particular to a traditional Chinese medicine microecological preparation and a preparation method thereof. The traditional Chinese medicine microecological preparation for preventing and treating enteritis of grass carp comprises the following components in percentage by weight: 25-35% of bacillus subtilis, 30-40% of bacillus aceticus and 20-35% of clostridium butyricum. In the invention, the traditional Chinese medicine extracting solution is used for culturing microbes, and the cultured microecological preparation contains rich polysaccharide components, probiotics and metabolite thereof of acalypha australis, polygonum bydropiper L., purslane and the like. The compound traditional Chinese medicine microecological preparation as the feed additive can be used as a substitute for antibiotic, and the additive prepared into the feed of the grass carp is green and safe, can prevent and treat the enteritis of the grass carp, enhances the immunity and disease resistance, can improve the conversion rate of the feed, and is favorable for purifying water and protecting and improving the ecological environment.

Description

A kind of Chinese medicine probiotics of preventing and treating grass carp enteritis and preparation method thereof
[technical field]
The present invention relates to a kind of feed addictive and preparation method thereof, relate in particular to a kind of Chinese medicine probiotics and preparation method thereof.
[background technology]
In recent years, along with the continuous expansion of propagating scale artificially, breeding environment has the trend that worsens day by day, and the outburst of disease is also more and more frequent, has greatly limited the growth of aquaculture output and the development of trade.From early 1950s FDA (FDA) ratify first antibiotic as feed addictive after, the antibiotics feed addictive begins to be used in a large number animal-breeding and produces.Antibiotic extensive application has brought abundant profit for breed enterprise, has caused more and more significantly negative effect also for simultaneously the sound development of feed industry, aquaculture and human beings'health.A host of facts prove, use antibiotic to make the animal body immunity degradation for a long time, cause that indirectly animal output and quality descend, and the raiser is sustained a loss, and " three cause " effect that the antibiotic of abuse and excess use simultaneously causes has had a strong impact on consumer's health.China's food security situation is very severe at present, especially China's aquatic product quality safety problem, pollution, water filling, poisonous, nuisance is residual, add problems such as forbidden drug, animal epidemic in the feed allows the consumer lack the sense of security, how to improve quality, reduce gradually and alternative antibiotic etc is made additive and used, become China's culture fishery development and press for one of major issue of solution, the nuisanceless green aquatic products of exploitation safety, high-quality, nutrition have become the common recognition of current society.
Developing the control that the Chinese medicine probiotics carries out the aquiculture animal disease with Chinese herbal medicine in conjunction with the method for biofermentation is the new method of rising in recent years, have nontoxic, have no side effect, the advantage of noresidue.The microniological proudcts that probiotics is based on the microecological balance theory, little ecoalimental is theoretical and grow up, it is controls of a kind of active form to the control of Animal diseases.The application of Chinese medicine probiotics in aquatic feeds, can improve the albumen accumulation in animal body in the feed on the one hand, reduce nitrogen and the discharging of phosphorus in water body environment, air and water environment had important function, the minimizing of the use amount of antibiotic and chemical synthetic drug can make the harmful metabolite in the environment reduce on the other hand, especially reduce the injury effect of medicament residue,, guarantee that people's quality of life is significant for ensuring food safety to human body.
[summary of the invention]
The purpose of this invention is to provide a kind of alternative antibiotic use; the effectively generation of prevention and treatment grass carp enteritis; improve immunity, the premunition of grass carp; and can improve the conversion ratio and the grass carp growth performance of feed; help purifying water, protect and the Chinese medicine probiotics of improving the ecological environment and preparation method thereof.
For achieving the above object, the technical solution used in the present invention is as follows:
A kind of Chinese medicine probiotics of preventing and treating grass carp enteritis contains following components in weight percentage: bacillus subtilis 25~35%, acetobacter 30~40%, clostridium butyricum 20~35%.
Preferably, contain following components in weight percentage: bacillus subtilis 35%, acetobacter 35%, clostridium butyricum 30%.
Described bacillus subtilis, acetobacter, clostridium butyricum are to be 5~8: 2~4 by weight ratio: 1.5~3 acalypha copperleaf, water polygonum flaccidum, purslane get through cultivation and fermentation.
Preferably, described bacillus subtilis, acetobacter, clostridium butyricum are to be that 6: 2.5: 1.5 acalypha copperleaf, water polygonum flaccidum, purslane gets through cultivation and fermentation by weight ratio.
A kind of method for preparing the Chinese medicine probiotics of above-mentioned control grass carp enteritis is got acetobacter, bacillus subtilis, clostridium butyricum and is mixed in proportion, and regulates PH scope to 3~4 with acetic acid, promptly gets Chinese medicine probiotics of the present invention.
Preferably, among the above-mentioned preparation method, regulate PH to 3.5 with acetic acid.
Above-mentioned Chinese medicine probiotics can intactly add in the feed as a kind of green feed additive, and its addition in feed is 0.5~1% of a feed gross weight, and preferred 0.5%.
In the Chinese medicine probiotics of the present invention, comprise bacillus subtilis, acetic acid bacillus and clostridium butyricum, wherein bacillus subtilis contains amylase and protease activities, in growth course, produce gemma, withstand high temperatures, reproduction speed is fast, can promote growth of animal, improve efficiency of feed utilization, keep the water body balance of nature; The acetic acid bacillus can make carbohydrate become the brevibacterium of products such as acetic acid with alcohol oxidation, can be in the environment of hypoxemia growth and breeding; Clostridium butyricum can enter the enteron aisle of aquatic livestock, suppress growth of pathogenic bacteria, regulate the intestinal microecology balance, can also promote histiocytic regeneration of gut epithelium and reparation simultaneously, improve the resistivity of animal, promote digesting and assimilating of bait disease.
The present invention compared with prior art, have following advantage: (1) cultivates microorganism with Chinese medicine extract, cultivate the probiotics that comes out and be rich in polysaccharide component, probio and metabolites thereof such as acalypha copperleaf, water polygonum flaccidum, purslane, the compound Chinese medicine probiotics can be used as feed addictive and substitutes antibiotic use, make feed of grass carp with this kind additive, green safety, can treat and prevention grass carp enteritis, strengthen immunity of organisms, premunition, and can improve the conversion ratio of feed, also help purifying water, protect and improve the ecological environment; (2) contained three kinds of microorganisms of acetobacter preparation, bacillus subtilis formulation, the clostridium butyricum preparation performance synergy aspect enhancing grass carp resistance against diseases and breeding water body purification of the present invention, realized the best effectively proportioning of compound micro-ecological preparation, this proportioning is proper rationally, can give full play to three's synergistic function; Contained microbial reproduction ability is strong in the preparation, easily forms useful dominant bacteria in breeding water body; Adjust aspects such as the inherent nutrition of animal body, immunity, growth stimulation, biological antagonist by profitable strain in the preparation and bringing into play the microecological balance of important effect, suppress the growth of pathogenic bacteria, promote the growth of animal self beneficial microorganism, strengthen the capacity of self-regulation of its little ecology, can also pass through the decomposition of water body harmful substance, the inhibition of pathogen and the cultivation of water body profitable strain, be used for improving water quality, keep the water ecology balance.
Product appearance of the present invention is light brown particle, and the special odor of fermentate is arranged.The survival rate of bacterium is all more than 90% in the probiotics, can finely play and strengthen animal immunizing power, raising digestibility, promote growth of animal, particularly can make the survival rate of grass carp improve more than 50% to the disease of digestive tract of grass carp, but disease of digestive tracts such as excellent prevention and treatment grass carp enteritis, compare the output that can make fish with similar feed and improve more than 20%, the user increases economic benefit 20%~40%.
[specific embodiment]
The invention will be further described below in conjunction with specific embodiment.
Embodiment 1
Chinese medical extract medium component proportioning is: in weight portion, and 50 parts of acalypha copperleafs, 30 parts of water polygonum flaccidums, 20 parts of purslanes.After cultivation and fermentation, bacillus subtilis 35%, acetobacter preparation 40%, clostridium butyricum 25% are mixed in proportion, and promptly get Chinese medicine probiotics of the present invention with acetic acid adjusting PH to 3.5.
During use, the compound micro-ecological preparation that present embodiment is made adds in the feed to get final product to according to 0.5% of feed weight.
Embodiment 2
Chinese medical extract medium component proportioning is: in weight portion, and 55 parts of acalypha copperleafs, 30 parts of water polygonum flaccidums, 15 parts of purslanes.After cultivation and fermentation, bacillus subtilis 25%, acetobacter preparation 40%, clostridium butyricum 35% are mixed in proportion, and promptly get Chinese medicine probiotics of the present invention with acetic acid adjusting PH to 3.5.
During use, the compound micro-ecological preparation that present embodiment is made adds in the feed to get final product to according to 0.5% of feed weight.
Embodiment 3
Chinese medical extract medium component proportioning is: in weight portion, and 60 parts of acalypha copperleafs, 25 parts of water polygonum flaccidums, 15 parts of purslanes.After cultivation and fermentation, bacillus subtilis 35%, acetobacter preparation 35%, clostridium butyricum 30% are mixed in proportion, and promptly get Chinese medicine probiotics of the present invention with acetic acid adjusting PH to 3.5.
During use, the compound micro-ecological preparation that present embodiment is made adds in the feed to get final product to according to 0.5% of feed weight.
Embodiment 4
Chinese medical extract medium component proportioning is: in weight portion, and 65 parts of acalypha copperleafs, 20 parts of water polygonum flaccidums, 15 parts of purslanes.After cultivation and fermentation, bacillus subtilis 30%, acetobacter preparation 40%, clostridium butyricum 30% are mixed in proportion, and promptly get Chinese medicine probiotics of the present invention with acetic acid adjusting PH to 4.0.
During use, the compound micro-ecological preparation that present embodiment is made adds in the feed to get final product to according to 0.5% of feed weight.
Embodiment 5
Chinese medical extract medium component proportioning is: in weight portion, and 50 parts of acalypha copperleafs, 25 parts of water polygonum flaccidums, 25 parts of purslanes.After cultivation and fermentation, bacillus subtilis 35%, acetobacter preparation 30%, clostridium butyricum 35% are mixed in proportion, and promptly get Chinese medicine probiotics of the present invention with acetic acid adjusting PH to 3.8.
During use, the compound micro-ecological preparation that present embodiment is made adds in the feed to get final product to according to 0.5% of feed weight.
Embodiment 6
The compliance test result test
1, test material: 11 circulation aquariums, remove contamination 25~30 ℃ of water temperatures, PH7~7.5, the grass carp fingerling of raising susceptible in the test with siphonage.
2, test method:, be respectively charged into number consecutively and be in 1~No. 11 the aquarium with each 30 tail of the grass carp of natural occurrence.Extraction is with the grass carp of wholesale disease, and visual inspection fish body colour is turned black, and body surface is slightly congested.Cut open the hyperemia of the visible fish intestines wall of inspection, intestines wall elasticity is relatively poor, and mucus is more in the enteric cavity, and red blood cell is less in the visible enteric cavity of microscopy, the epithelial cell that has partial necrosis to come off.Laboratory diagnosis is that the disease fish is the enteritis that point-like aerogenesis pseudomonas bacillus causes.
3, with the feed of grass carp of 1 and 2 group, 3 and 4 groups, 5 and 6 groups, 7 and 8 groups, the 9 and 10 groups Chinese medicine probioticses that contain example 1,2,3,4,5 preparation of throwing something and feeding respectively, throw something and feed 11 groups of blank bait of throwing something and feeding every day 2 times.The grass carp adult fish material 801A that the blank bait of control group selects for use the Hai Wei Co., Ltd., Guangzhou City to produce.
4, above each test group is all observed and is raised 20d, and record fish body is ingested and dead, survival condition.
5, result of the test: result of the test sees Table
Show as can be seen thus, embodiment 3, and soon bacillus subtilis 35%, acetobacter preparation 35%, clostridium butyricum 30% mix in proportion, and promptly get Chinese medicine probiotics of the present invention with acetic acid adjusting PH to 3.5.The Chinese medicine probiotics that embodiment 3 is made adds in the feed according to 0.5% of feed weight, and the feed of grass carp raising survival rate of making is the highest, and effect is best.

Claims (8)

1. a Chinese medicine probiotics of preventing and treating grass carp enteritis is characterized in that containing following components in weight percentage: bacillus subtilis 25~35%, acetobacter 30~40%, clostridium butyricum 20~35%.
2. the Chinese medicine probiotics of control grass carp enteritis as claimed in claim 1 is characterized in that containing following components in weight percentage: bacillus subtilis 25%, acetobacter 35%, clostridium butyricum 30%.
3. the Chinese medicine probiotics of control as claimed in claim 1 or 2 grass carp enteritis, it is characterized in that described bacillus subtilis, acetobacter, clostridium butyricum are is 5~8: 2~4 by weight ratio: 1.5~3 acalypha copperleaf, water polygonum flaccidum, purslane get through cultivation and fermentation.
4. the Chinese medicine probiotics of control as claimed in claim 3 grass carp enteritis is characterized in that described bacillus subtilis, acetobacter, clostridium butyricum are is that 6: 2.5: 1.5 acalypha copperleaf, water polygonum flaccidum, purslane gets through cultivation and fermentation by weight ratio.
5. one kind prepares method of preventing and treating the Chinese medicine probiotics of grass carp enteritis as claimed in claim 1, it is characterized in that getting acetobacter, bacillus subtilis, clostridium butyricum mixes in proportion, and, promptly get Chinese medicine probiotics of the present invention with acetic acid adjusting PH scope to 3~4.
6. the method for the Chinese medicine probiotics of control grass carp enteritis as claimed in claim 5, it is characterized in that getting acetobacter, bacillus subtilis, clostridium butyricum mixes in proportion, and, promptly get Chinese medicine probiotics of the present invention with acetic acid adjusting PH scope to 3.5.
7. a using method of preventing and treating the Chinese medicine probiotics of grass carp enteritis according to claim 1 is characterized in that its addition in feed is 0.5~1% of a feed gross weight.
8. the using method of the Chinese medicine probiotics of control grass carp enteritis as claimed in claim 7 is characterized in that its addition in feed is 0.5% of a feed gross weight.
CN2010105630625A 2010-11-24 2010-11-24 Traditional Chinese medicine microecological preparation for preventing and treating enteritis of grass carp and preparation method thereof CN101983582B (en)

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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102367417A (en) * 2011-09-16 2012-03-07 汪松林 Traditional Chinese medicine probiotics liquid medium used for fish feed, culturing method and feed additive
CN102366025A (en) * 2011-09-16 2012-03-07 汪松林 Method for preparing grass carp feed additive containing traditional Chinese medicine active probiotics
CN103583928A (en) * 2013-12-02 2014-02-19 郝智慧 Feed used for improving immune functions of grass carp
CN103609848A (en) * 2013-11-26 2014-03-05 青岛嘉瑞生物技术有限公司 Fermented Chinese herbal medicine feed additive for aquatic products and preparation method thereof
CN104054940A (en) * 2014-05-27 2014-09-24 范治淮 Disease-resistant grass carp feed and preparation method thereof
CN104248701A (en) * 2014-09-26 2014-12-31 浙江海洋学院 Fish medicine for treating enteritis of grass carps and preparation method of fish medicine
CN104404103A (en) * 2014-12-17 2015-03-11 成都乾坤动物药业有限公司 Method for increasing content of portulaca oleracea polysaccharide and fermentation broth prepared with method
CN105746947A (en) * 2016-03-07 2016-07-13 五河县金满塘生态种养殖家庭农场 Microecological preparation for improving intestinal function of Elopichthys bambusa
CN105994944A (en) * 2016-06-01 2016-10-12 蚌埠市宝煦家庭农场 Feed capable of preventing and curing enteritis of megalobrama amblycephala
CN106107045A (en) * 2016-07-22 2016-11-16 安徽源丰农业发展有限公司 A kind of biological feedstuff preventing and treating Misgurni anguillicaudati enteritis disease
CN106954761A (en) * 2017-03-30 2017-07-18 广州市海维饲料有限公司 A kind of ecological feed for repairing grass carp intestinal villus and preparation method thereof
CN107319196A (en) * 2017-07-20 2017-11-07 通化师范学院 A kind of microorganism formulation for improving fresh-water fishes function of intestinal canal and application thereof
CN107810909A (en) * 2017-11-09 2018-03-20 梁平县西科农业发展有限公司 A kind of mixed breed additive for improving grass carp survival rate and preparation method thereof
CN108567845A (en) * 2018-06-11 2018-09-25 广东省农业科学院动物卫生研究所 A kind of biofermentation products and preparation method thereof of prevention colitis
CN109125643A (en) * 2018-08-17 2019-01-04 北海市绿滴生物科技有限公司 A kind of fermented tcm and preparation method thereof preventing and treating aquatic products intestines problem

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Publication number Priority date Publication date Assignee Title
CN102366025A (en) * 2011-09-16 2012-03-07 汪松林 Method for preparing grass carp feed additive containing traditional Chinese medicine active probiotics
CN102366025B (en) * 2011-09-16 2013-01-23 汪松林 Method for preparing grass carp feed additive containing traditional Chinese medicine active probiotics
CN102367417A (en) * 2011-09-16 2012-03-07 汪松林 Traditional Chinese medicine probiotics liquid medium used for fish feed, culturing method and feed additive
CN103609848A (en) * 2013-11-26 2014-03-05 青岛嘉瑞生物技术有限公司 Fermented Chinese herbal medicine feed additive for aquatic products and preparation method thereof
CN103583928B (en) * 2013-12-02 2015-03-25 青岛百慧智业生物科技有限公司 Feed used for improving immune functions of grass carp
CN103583928A (en) * 2013-12-02 2014-02-19 郝智慧 Feed used for improving immune functions of grass carp
CN104054940A (en) * 2014-05-27 2014-09-24 范治淮 Disease-resistant grass carp feed and preparation method thereof
CN104248701A (en) * 2014-09-26 2014-12-31 浙江海洋学院 Fish medicine for treating enteritis of grass carps and preparation method of fish medicine
CN104404103B (en) * 2014-12-17 2017-08-18 成都乾坤动物药业有限公司 Zymotic fluid prepared by a kind of method and this method for improving Polysaccharide from Portulaca oleracea
CN104404103A (en) * 2014-12-17 2015-03-11 成都乾坤动物药业有限公司 Method for increasing content of portulaca oleracea polysaccharide and fermentation broth prepared with method
CN105746947A (en) * 2016-03-07 2016-07-13 五河县金满塘生态种养殖家庭农场 Microecological preparation for improving intestinal function of Elopichthys bambusa
CN105994944A (en) * 2016-06-01 2016-10-12 蚌埠市宝煦家庭农场 Feed capable of preventing and curing enteritis of megalobrama amblycephala
CN106107045A (en) * 2016-07-22 2016-11-16 安徽源丰农业发展有限公司 A kind of biological feedstuff preventing and treating Misgurni anguillicaudati enteritis disease
CN106954761A (en) * 2017-03-30 2017-07-18 广州市海维饲料有限公司 A kind of ecological feed for repairing grass carp intestinal villus and preparation method thereof
CN107319196A (en) * 2017-07-20 2017-11-07 通化师范学院 A kind of microorganism formulation for improving fresh-water fishes function of intestinal canal and application thereof
CN107810909A (en) * 2017-11-09 2018-03-20 梁平县西科农业发展有限公司 A kind of mixed breed additive for improving grass carp survival rate and preparation method thereof
CN108567845A (en) * 2018-06-11 2018-09-25 广东省农业科学院动物卫生研究所 A kind of biofermentation products and preparation method thereof of prevention colitis
CN109125643A (en) * 2018-08-17 2019-01-04 北海市绿滴生物科技有限公司 A kind of fermented tcm and preparation method thereof preventing and treating aquatic products intestines problem

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