CN105961922A - Feed for regulating and controlling sea water acidification stress of fishes - Google Patents
Feed for regulating and controlling sea water acidification stress of fishes Download PDFInfo
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- CN105961922A CN105961922A CN201610420315.0A CN201610420315A CN105961922A CN 105961922 A CN105961922 A CN 105961922A CN 201610420315 A CN201610420315 A CN 201610420315A CN 105961922 A CN105961922 A CN 105961922A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention provides a feed for regulating and controlling sea water acidification stress of fishes. The feed comprises a basal feed and additives; the basal feed is prepared from 780-820 parts of protein source materials, 9-11 parts of granulesten, 60-90 parts of starch, 45-55 parts of fish oil, 13-17 parts of mineral mixture and 13-17 parts of vitamin mixture; the additives comprise 25-75 parts of cortex eucommiae powder, 3-12 parts of chitosan oligosaccharide, 0.05-0.15 part of tea tree oil, 2.5-10 parts of alanyl-glutamine, 0.01-0.05 part of vitamin E, 3-12 parts of citric acid and 0.0002-0.001 part of selenium. According to the feed for regulating and controlling the sea water acidification stress of the fishes, alanyl-glutamine and vitamin E are added, the functions such as digestion, absorption, metabolism, oxidation resistance and immune property of the fishes are effectively regulated and controlled, sea water acidification stress reactions are relieved, and defense, disease resistance and growth and development capacities are improved. One or two of citric acid and selenium are added, adjustment of functions such as nerves, internal secretion, metabolism, oxidation resistance and the immune property of the fishes is further enhanced, the sea water acidification stress reactions of the fishes are reduced, damage to the fishes is reduced, and sustainable growth and development of the fishes are guaranteed.
Description
Technical field
The present invention relates to fish meal field.More specifically, the acidifying of one of the present invention regulation and control fish sea water stress
Feedstuff.
Background technology
Since the industrial revolution, due to mankind's activity and industrial and agricultural development, the carbon dioxide level in air
Rising with unprecedented speed, the amount of water body absorbing carbon dioxide is continuously increased, and causes water acidification,
Water body pH declines.Water acidification causes the aquatic animal Acid stress reactions such as Fish, has a strong impact on its soda acid
Balance, metabolism, hormone secretion, skeleton development and immunologic function etc., even threaten aquatic animal
Existence, group and ecology, therefore need research one regulation and control Fish Acid stress metabolism badly, alleviate Acid stress pair
The feedstuff of the damage of Fish.
Summary of the invention
It is an object of the invention to solve at least the above or defect, and offer at least will be described later
Advantage.
It is a still further object of the present invention to provide a kind of regulate and control fish sea water acidifying stress feedstuff, it is on basis
Feedstuff adds alanyl glutamine and vitamin E, the metabolism of Effective Regulation fish, alleviates acid
The stress damage to Fish.
It is a still further object of the present invention to provide a kind of regulate and control fish sea water acidifying stress feedstuff, it is on basis
Feedstuff adds Cortex Eucommiae powder, oligochitosan and tea tree oil, strengthens the body constitution of fish, growth rate and immunity energy
Power, to improve the sea water Acid stress of fish.
It is a still further object of the present invention to provide a kind of regulate and control fish sea water acidifying stress feedstuff, it is on basis
Feedstuff adds citric acid and selenium, improves oxidation resistance, immunocompetence and protective capacities.
In order to realize according to object of the present invention and further advantage, it is provided that a kind of regulation and control fish sea water acid
Change stress feedstuff, wherein, including normal feedstuff part and additive part；
Wherein said normal feedstuff part mainly includes following components: protein sources material 780-820, Semen sojae atricolor phosphorus
Fat 9-11, starch 60-90, fish oil 45-55, mineral mixture 13-17 and vitamin mixtures 13-17；
Described additive part mainly includes following components: Cortex Eucommiae powder 25-75, oligochitosan 3-12, tea tree oil
0.05-0.15, ALA-GLN 2.5-10, vitamin E 0.01-0.05.
Preferably, described regulation and control fish sea water acidifying stress feedstuff in, described additive part is also wrapped
Include the one in following component or several: citric acid 3-12, selenium 0.0002-0.001.
Preferably, described regulation and control fish sea water acidifying stress feedstuff in, described protein sources material is main
Comprise the one in following component or several: Erythroculter ilishaeformis powder 550-570, casein 40-55 and Semen sojae atricolor
160-210。
Preferably, described regulation and control fish sea water acidifying stress feedstuff in, described vitamin mixtures bag
Containing one or more in following component: vitamin A 60-100, vitamin D330-50, vitamin
K30.1-0.15, vitamin B10.1-0.2, vitamin B20.2-0.4, vitamin B60.2-0.4, vitamin
B120.001-0.003, nicotinic acid 0.01-0.02, Potassium pantothenate. 0.5-0.8, folic acid 0.02-0.05, biotin
0.001-0.003, inositol 1-3, vitamin C 0.08-0.12 and choline chloride 8-12；
Described regulation and control fish sea water acidifying stress feedstuff in, described mineral mixture comprises following component
In one or more: ferrous sulfate 120-200, zinc sulfate 500-700, manganese chloride 30-50, sulphuric acid
Copper 150-260, iodine 8-12, magnesium chloride 150-260, cobaltous sulfate 15-25 and sodium molybdate 15-25.
Preferably, described regulation and control fish sea water acidifying stress feedstuff in, main in every kilogram of dry feed
Including following components:
Erythroculter ilishaeformis powder 560g, casein 50g, Semen sojae atricolor 160g, soybean phospholipid 9g, starch 75g, fish oil 50g,
Mineral mixture 15g, vitamin mixtures 15g, Cortex Eucommiae powder 50g, oligochitosan 6g, tea tree oil 0.05g,
ALA-GLN 5g, vitamin E 0.02g, citric acid 6g, selenium powder 0.0009g.
Preferably, described regulation and control fish sea water acidifying stress feedstuff in, main in every kilogram of dry feed
Including following components:
Erythroculter ilishaeformis powder 556g, casein 45g, Semen sojae atricolor 180g, soybean phospholipid 10g, starch 85g, fish oil
45g, mineral mixture 14g, vitamin mixtures 14g, Cortex Eucommiae powder 25g, oligochitosan 12g, Camellia sinensis
Oil 0.1g, ALA-GLN 7.5g, vitamin E 0.03g, citric acid 9g, selenium powder 0.0006g.
Preferably, described regulation and control fish sea water acidifying stress feedstuff in, main in every kilogram of dry feed
Including following components:
Erythroculter ilishaeformis powder 551g, casein 40g, Semen sojae atricolor 170g, soybean phospholipid 11g, starch 70g, fish oil
48g, mineral mixture 13g, vitamin mixtures 13g, Cortex Eucommiae powder 75g, oligochitosan 3g, Camellia sinensis
Oil 0.15g, ALA-GLN 3g, vitamin E 0.04g, citric acid 3g, selenium powder 0.0003g.
Preferably, described regulation and control fish sea water acidifying stress feedstuff in, the molecular weight of described oligochitosan
For 500-550, the degree of polymerization is 4-5；Described selenium be particle diameter be the nanometer selenium of 500-600nm.
The present invention at least includes following beneficial effect:
First, of the present invention can regulate and control fish sea water acidifying stress feedstuff, it adds in normal feedstuff
Adding Eucommia Leaf Powder, oligochitosan, tea tree oil, alanyl glutamine and vitamin E, normal feedstuff can
There is provided sufficient nutritional labeling for fish, additive can the metabolism of Effective Regulation fish, improve fish antioxygen
Change, immunity and repair ability, alleviate the Acid stress reaction infringement to Fish, make fish healthy development grow up.
The Cortex Eucommiae contains the medicinal ingredients such as chlorogenic acid, Folium Persicae coral and (+)-Pinoresinol glucosulfone examination, and protein,
The nutritional labeling such as vitamin and mineral element, has invigorating the liver and kidney, bone and muscle strengthening, antitumor, anti-inflammation and sterilization etc.
Effect of many mysteries, the Cortex Eucommiae can significantly improve the immunologic function of animal body, the defence capability of enhancing body,
Cellular immunization had dual regulation.Select Folium Eucommiae more can reduce the production cost of feedstuff.
Oligochitosan can promote growth of animal, improve immunity, blood fat reducing, antibacterial, antiinflammatory and anticancer etc..
In feedstuff, add appropriate oligochitosan can significantly improve the speed of growth of fish and anti-infected by Aeromonas hydrophila
Ability.Oligochitosan can also improve the peroxidase activity of hemolymph to some extent.Use oligochitosan
Immunoglobulin quantity in blood can be made to increase, improve immunity of organisms.Oligochitosan can also blood sugar lowering,
And have no side effect, it is a kind of pollution-free, feed additive of noresidue.Oligochitosan additive can be notable
Promotion Mylopharyngodon piceus juvenile fish liver and the expression of intestinal IL-2, IL-8 and IL-10m RNA, proinflammatory inflammation factor IL-2,
The expression of IL-8 and anti-inflammatory cytokine IL-10 improves simultaneously, can effectively promote the anti-inflamatory levels of body, and
Body will not be produced unfavorable factor, it reason is that IL-2 is SCIF, can promote that T is thin
Born of the same parents' immunoreation, energy activating T cell and the expression of macrophage, promote various kinds of cell reaction, promote to drench
The killing cell differentiation of bar factor activator and immunoglobulin,exocrine (Ig)；IL-8 is typical proinflammatory disease
The factor, is also a kind of important chemotactic factor, also has the strongest angiogenesispromoting effect, and IL-8 regulates inflammation
Disease is reacted, and infected group is woven with certain restitution；IL-10 has antiinflammatory, antitumor action, participates in
Inflammatory reaction also can alleviate inflammation, and body is had protective effect, has immunomodulating and make in bony fish
With, the expression increase of IL-10mRNA can suppress inflammatory reaction, alleviates inflammation disease, contributes to treatment inflammation
Disease.Meanwhile, the activity of lactic acid dehydrogenase (LDH) during oligochitosan significantly reduces Mylopharyngodon piceus juvenile fish serum.Should
The reduction of enzymatic activity is conducive to protection fish body tissue, maintains LDH balance.Oligochitosan can also significantly reduce
The content of Mylopharyngodon piceus juvenile fish Triglycerides in Serum, serum triglycerides reduces makes blood fat reduce, and is of value to the heart
Vascular health and performance physiological function.
Tea tree oil is a plant-derived feed additive of class, and it is mainly composed of essence pool.Tea tree oil is permissible
By selective bacterium being suppressed thus the ecological balance of beneficially archenteric flora, improve animal intestinal micro-
Biotic environment, it is ensured that intestinal health, improves animal and absorbs nutritional labeling；Tea tree oil also has
Anti-inflammatory, antibacterial and the effect of raising immunity, have antifungal and preservation, and tea tree oil also has alleviation
Diarrhoea, promotes the effect of growth, and tea tree oil can use between the animal egg-laying season, noresidue, no
Animal can be caused bad shadow.
Vitamin E (VE) has many physiological functions, most important of which is that and affects immunity of organism, anti-
Oxidation, grow and breeding etc., highly unsaturated fatty acid (HUFAs) can be protected from oxidation and to remain important
Signal conversion needed for HUFAs concentration.VE has special film function, to multiple enzymatic activity, letter
Number conversion and VE response gene expression etc. are adjusted.But the required trace battalion that VE is body can not be synthesized
Support element and antioxidant, food intake can only be passed through.Add VE and can improve the phagocyte activity of fish,
It is obviously enhanced the phagocytic activity of carp, dramatically increases superoxide dismutase (SOD) and reduce fat peroxide
Compound-malonaldehyde (MDA).VE can also inhibited apoptosis, increase its survival rate etc..
Add alanyl glutamine (AGD) and the defence capability of fish can be significantly improved, improve non-specific
Immunity.AGD is the main source of body exogenous glutamine (Gln).Owing to the dissolubility of Gln is low
Unstable with character, therefore replace Gln with AGD.Safe and effective, with the physiological action of Gln with AGD
It is as good as, and the AGD taken in also can discharge Gln and alanine confession in internal through dipeptidase hydrolysis simultaneously
Tissue metabolism uses.Gln is conditionally essential amino acid when physical stress, injured, disease.Stress,
Under the states such as injured, disease, the requirement of Gln is increased by body, and self synthesis supply falls short of demand, must
External source Gln must be increased；Supplement Gln timely and appropriately and can improve sick body.In daily ration, add Gln can promote
Enter the growth of fish, the growth of intestinal and perfect in shape and function, increase glutathion peroxidase (GPx), super oxidation
Thing dismutase (SOD), the content of glutathion (GSH), improve digestive enzyme, Na+/K+-ATPase and SOD
Activity, reduce lipoperoxide-malonaldehyde (MDA) and feed coefficient.Gln can also reduce proinflammatory disease
The expression of cytokine, improves intestinal barrier function, improves stress ability, improves immune cell function
With reduction mortality rate；Gln is apoptotic inhibitor.In feedstuff, add Gln, the anti-of fish can be improved
Poison ability, increases survival rate.Gene expression that Gln scalable fish is relevant to metabolism, signal transduction, thin
Born of the same parents' defence and repairing, also can signal path in active cell.Gln has at multiple organ and histiocyte
Unique and important metabolic function, and can maintain intestinal structure and barrier function, alleviates endotoxin etc. to intestinal
The damage in road, strengthens the expression of heat shock protein 70 (HSP70) gene.HSP70 is body in stress be anti-
The protein produced in should, its great expression can improve the stress ability of cell, resists various harmful factor
Damage.Gln is not only one of metabolic fuel of living cells, and is that nucleic acid and protein etc. are raw
The precursor of thing synthesis.Therefore, Gln has important effect to the propagation of cell.Gln is as immunocyte
One of required material of division growth, can improve body B cell function, promotes B cell secretory antibody,
Strengthen intestinal and general immunity, strengthen human body defence capability.
Eucommia Leaf Powder, oligochitosan, tea tree oil, alanyl glutamine and vitamin E can complementary action,
Synergistic.Gln is one of Fish important nutrient, can generate antioxidant GSH.VE is fat-soluble
Chain breaking antioxidants, it affects the balance between GSH oxidoreduction, oxidant and antioxidant.Combination
Gln and VE, on the one hand can remove singlet oxygen (1And superoxide anion (O O)2 -), on the other hand can be with paddy
Guang sweet peptide peroxidase (GPx) synergism, blocks lipid peroxidation chain, protecting film structure and lipoprotein
Biological activity.Therefore Gln with VE is closely related in terms of anti-oxidation stress, can complementary and collaborative increasing
Effect.Gln and VE has facilitation to growth, growth, antioxidation and the immunity of Fish, and the two is altogether
With reduction fry cell stress can be worked in coordination with, improve cells survival developmental capacity.
Secondly, the feedstuff that fish Acid stress can be regulated and controled of the present invention, also added citric acid and selenium.
Selenium (Se) is played important biochemical physiological action by digestive tract after bio-absorbable, major function can be general
State as (1) antioxidation；(2) immunization；(3) protection, health-care effect.
Citric acid (CA) affects the enzyme of Fish, metabolism, health, grows, grows and survive.CA is to ocean
Biological enzyme system plays material impact, and it is digested and assimilated, metabolism, immunity and the growth important work of performance
With.Adding CA in daily ration to be greatly improved the growth of Fish, grow and survive, its dominant mechanism can
Can be that CA reduces fish stomach pH, activate pepsin, promote the digestion of nutrient substance, absorb and utilize.
CA is the key substance of body mitochondrion tricarboxylic acid cycle, participate in and activation wire plastochondria in tricarboxylic acid cycle and
Oxidative phosphorylation, produces more adenosine triphosphate (ATP) for body.The antioxidation of CA, a side
Face CA, as antioxidative synergist, promotes the antioxidant activity of corresponding antioxidant；On the other hand CA
The metal ion of prooxidant effect can be chelated, formed the chelate not having oxidation activity, cut off gold
Belong to the lipid hydroperoxide free chain reaction that ion causes.
Additionally, VE Yu Se is in addition to playing important physiological function respectively, can complementary and Synergistic.VE
Antioxidation and anti allergic reaction can be worked in coordination with Se.As added VE and nanometer Se in feedstuff, can improve dynamic
GPx enzymatic activity and total antioxidant capacity (T-AOC) in thing serum, reduce MDA and reactive oxygen species (ROS)
Level, reduces the heat stress oxidative damage to animal.VE can maintain the activity of internal Se, prevents Se
Discharge and membrane oxidation, reduce the generation of peroxide, thus save GPx and SOD for removing
Peroxide；VE can also strengthen the effect of Se, and prevention DNA destroys and carcinogenic.VE and Se can
Increase body serum anti-oxidant vigor, particularly significant enhancing catalase (CAT), GPx and paddy Guang
The vigor of sweet fabk polypeptide (GR), resists oxidative damage.VE Yu Se can work in coordination with raising immunity and resist
Sick power.Appropriate interpolation Se and VE, can significantly improve natural killer cell (NK cell) activity and T
Lymhocyte transformation rate.Se and VE can work in coordination with raising body antibody horizontal, phagocyte and lymphocyte
Function, strengthen premunition.VE and Se is outer born of the same parents respectively and intracellular plays immunity antioxidation.Two
Person is complementary, synergism.
CA and VE also can work in coordination with antioxidation potentiation.CA can chelated metal ions and suppress its be catalyzed lipid
Oxidation, reduces and produces free radical, thus reduce and remove the VE that free radical is consumed, protect and improve VE
Antioxidant effect.Combination VE and CA can dramatically increase the quantity of immune cell, is greatly improved
Immunity, significantly increases the oxidation resistance of body.CA provides the acidity suitably played a role for VE
Environment, VE is stable at sour environment, can preferably play antioxidation.CA makes VE regenerate, and
VE protects CA.
Selenium is made the nano-particle that particle diameter is 500-600nm and can be effectively improved the absorbance of selenium.
Finally, use the sea water that can regulate and control fish acidifying of the present invention stress feedstuff after, the weightening finish of fish
Rate (BWG%), specific growth rate (SGR%), T-AOC, GPx, CAT and SOD content or
Activity is the highest, and malondialdehyde (MDA) is minimum, so described feedstuff can be effective
Improving Fish oxidation resistance, promote metabolism, alleviating sea water acidifying stress be on the impact of Fish.
Part is embodied by the further advantage of the present invention, target and feature by description below, and part also will
By the research of the present invention and practice are understood by the person skilled in the art.
Detailed description of the invention
The present invention is described in further detail below, to make those skilled in the art with reference to description literary composition
Word can be implemented according to this.
Should be appreciated that used herein such as " have ", " comprising " and " including " term also
Do not allot other element one or more or the existence of a combination thereof or interpolation.
Example 1
Prepared by product: prepared by product A:
Step one, prepare normal feedstuff: by Erythroculter ilishaeformis powder 560g, casein 50g, Semen sojae atricolor 160g, Semen sojae atricolor
Phospholipid 9g, starch 75g, fish oil 50g, mineral mixture 15g and vitamin mixtures 15g mixing
It is uniformly mixing to obtain normal feedstuff.Wherein, mineral mixture includes: ferrous sulfate 120-200, sulphuric acid
Zinc 500-700, manganese chloride 30-50, copper sulfate 150-260, iodine 8-12, magnesium chloride 150-260, sulphuric acid
Cobalt 15-25 and sodium molybdate 15-25；Vitamin mixtures includes: vitamin A 60-100, vitamin D3
30-50, vitamin K30.1-0.15, vitamin B10.1-0.2, vitamin B20.2-0.4, vitamin B6
0.2-0.4, vitamin B120.001-0.003, nicotinic acid 0.01-0.02, Potassium pantothenate. 0.5-0.8, folic acid
0.02-0.05, biotin 0.001-0.003, inositol 1-3, vitamin C 0.08-0.12 and choline chloride
8-12。
Step 2, addition additive part: Cortex Eucommiae powder 0g, oligochitosan 0g, tea tree oil 0g, alanyl-
Glutamine 0g, vitamin E 0.04g, mix homogeneously obtain of the present invention have regulation and control fish Acid stress
The feedstuff A of function.
Prepared by product B: on the basis of using same normal feedstuff, adds additive part and is: Du
Secondary powder 25g, oligochitosan 3g, tea tree oil 0.05g, ALA-GLN 2.5g, vitamin E 0.03g,
Mix homogeneously obtains the feedstuff B with regulation and control fish Acid stress function of the present invention.
Prepared by products C: using on the basis of using same normal feedstuff, adding additive part
For: Cortex Eucommiae powder 50g, oligochitosan 6g, tea tree oil 0.1g, ALA-GLN 5g, vitamin E 0.02g,
Mix homogeneously obtains the feedstuff C with regulation and control fish Acid stress function of the present invention.
Prepared by product D: using on the basis of using same normal feedstuff, adding additive part
For: Cortex Eucommiae powder 75g, oligochitosan 12g, tea tree oil 0.15g, ALA-GLN 7.5g, vitamin
E 0.01g, mix homogeneously obtains the feedstuff D with regulation and control fish Acid stress function of the present invention.
Prepared by product E: using on the basis of using same normal feedstuff, adding additive part
For: Cortex Eucommiae powder 0g, oligochitosan 0g, tea tree oil 0g, ALA-GLN 10g, vitamin E 0g,
Mix homogeneously obtains the feedstuff E with regulation and control fish Acid stress function of the present invention.
Prepared by product F: feedstuff based on product F.
Contrast test:
This programme object of study is that sea water is blue or green (Oryzias melastigma), is the research sea water of new development
It is biological that fish diseases manages toxicologic ideal model.Sea water is blue or green in marine sites such as China, Korea, Japan, India
Generally exist, mild, spawning rate is high, growth cycle short (about 3 months), small (adult fish
Individuality is about 3.5-4.0cm), be prone to breed (the most all can breed), raise and observe, to environment
Changing extremely sensitive, the idealized model being by the research of Fish Acid stress is biological.
Sea water is blue or green close with the sibship of fresh water blue or green (Oryzias latipes), and growth course is identical.
The biology of fresh water green grass or young crops, nutritional need and full gene order-checking have been studied clear the most, and it for information about may be used
For sea water green grass or young crops reference, it is also possible to do same experiment with fresh water green grass or young crops.
Be utilized respectively A-F product feed fish, condition of breeding fish (salinity 30 ± 2, water temperature is 28 ± 2 DEG C,
PH is 8.10, dissolved oxygen amount >=6.10mg/L, and Light To Dark Ratio is 14h:10h) the most identical with experimental implementation.Often
They 200 mesh feedstuffs of throwing something and feeding for three times, total feeding volume is the 5% of the wet body weight of fish.Every day observes in detail and records son
Fish is movable, ingest, lopsided, dead etc., random acquisition fish sample after 8 weeks, weigh respectively, be stored in-80 DEG C
For molecule, gene, physiology and biochemical measurement in refrigerator.Result is as follows:
By the order of A-F；
The signal factor GPx gene relative expression quantity (2 of sea water prelarva-△△Ct) respectively 1.81,
2.01、2.66、2.21、2.04、1.10.Along with AGD and VE dosage increases, GPx gene table in fish body
Reach increase, when the addition of AGD and VE is respectively 5g/kg and 50IU/kg normal feedstuff, GPx base
Because expression is maximum.
The signal factor PPAR α gene relative expression quantity (2 of sea water prelarva-△△Ct) respectively 1.63,
1.75、1.91、1.90、1.84、0.98.Along with AGD and VE dosage increases, PPAR α in fish body
Gene expression also increases, when the addition of AGD and VE is respectively 5g/kg and 50IU/kg normal feedstuff
Time, PPAR α gene expression amount is maximum.
Rate of body weight gain (BWG%) is respectively 82.09,82.94,87.21,86.54,86.05,81.12.
Specific growth rate (SGR%) is respectively 4.28,4.31,4.45,4.43,4.40,4.24.
Malonaldehyde (MDA) content is respectively 1.82,1.67,1.08,1.38,1.51,1.34nmol/mgprot.
GPx content (active) be respectively 120.36,148.68,129.35,125.95,77.13U/mgprot.
CAT content (active) be respectively 2.65,2.84,3.09,2.80,2.78,2.41U/mgprot.
SOD content (active) be respectively 9.79,10.95,12.96,10.62,10.50,8.91U/mgprot.
T-AOC content (active) be respectively 1.09,1.13,1.19,1.12,1.10,1.04U/mg.
Sum it up, when the additive in every kilogram of basic dry feed is containing Cortex Eucommiae powder 25-75g, oligochitosan
When 3-12g, tea tree oil 0.05-0.15g, AGD 2.5-7.5g and VE 75-25IU (i.e. 0.05-0.025g),
The rate of body weight gain (BWG%) of fish, specific growth rate (SGR%), GPx, CAT, SOD and T-AOC
Content or activity are the highest, and malondialdehyde (MDA) is minimum, and institute of the present invention is described
The feedstuff stated can be effectively improved Fish oxidation resistance, promotes metabolism, alleviates the Acid stress impact on Fish.
Example 2
Prepared by product: prepared by product a:
Step one, prepare normal feedstuff: by Erythroculter ilishaeformis powder 560g, casein 50g, Semen sojae atricolor 160g, Semen sojae atricolor
Phospholipid 9g, starch 75g, fish oil 50g, mineral mixture 15g and vitamin mixtures 15g mixing
It is uniformly mixing to obtain normal feedstuff.Wherein, mineral mixture includes: ferrous sulfate 120-200, sulphuric acid
Zinc 500-700, manganese chloride 30-50, copper sulfate 150-260, iodine 8-12, magnesium chloride 150-260, sulphuric acid
Cobalt 15-25 and sodium molybdate 15-25；Vitamin mixtures includes: vitamin A 60-100, vitamin D3
30-50, vitamin K30.1-0.15, vitamin B10.1-0.2, vitamin B20.2-0.4, vitamin B6
0.2-0.4, vitamin B120.001-0.003, nicotinic acid 0.01-0.02, Potassium pantothenate. 0.5-0.8, folic acid
0.02-0.05, biotin 0.001-0.003, inositol 1-3, vitamin C 0.08-0.12 and choline chloride
8-12。
Step 2, add additive part: by Cortex Eucommiae powder 50g, oligochitosan 6g, tea tree oil 0.1g, third
After aminoacyl-glutamine 5g, vitamin E 0.03g, citric acid 0g, selenium powder 0g, mix homogeneously obtains this
The bright described feedstuff a with regulation and control fish Acid stress function.
Prepared by product b: on the basis of using same normal feedstuff, adds additive part and is: Du
Secondary powder 50g, oligochitosan 6g, tea tree oil 0.1g, ALA-GLN 5g, vitamin E 0.03g,
Citric acid 3g, selenium powder 0.0003g mix homogeneously obtain of the present invention have regulation and control fish Acid stress function
Feedstuff b.
Prepared by product c: using on the basis of using same normal feedstuff, adding additive part
For: Cortex Eucommiae powder 50g, oligochitosan 6g, tea tree oil 0.1g, ALA-GLN 5g, vitamin E 0.03g,
Citric acid 6g, selenium powder 0.0005g, mix homogeneously obtain of the present invention have regulation and control fish Acid stress merit
The feedstuff c of energy.
Prepared by product d: using on the basis of using same normal feedstuff, adding additive part
For: Cortex Eucommiae powder 50g, oligochitosan 6g, tea tree oil 0.1g, ALA-GLN 5g, vitamin E 0.03g,
Citric acid 9g, selenium powder 0.0008 mix homogeneously obtains of the present invention having regulation and control fish Acid stress function
Feedstuff d.
Prepared by product e: using on the basis of using same normal feedstuff, adding additive part
For citric acid 12g, selenium powder 0.001 mix homogeneously obtains of the present invention having regulation and control fish Acid stress merit
The feedstuff e of energy.
Prepared by product f: feedstuff based on product f.
Contrast test:
This programme object of study be sea water green grass or young crops be the research toxicologic ideal mode of marine fish pathology of new development
Type is biological.Green grass or young crops generally exists sea water in marine sites such as China, Korea, Japan, India, mild,
Spawning rate height, growth cycle short (about 3 months), small (adult fish individuality is about 3.5-4.0cm),
It is prone to breed (the most all can breed), raise and observe, extremely sensitive to environmental change, it is by fish
The idealized model of class Acid stress research is biological.
The blue or green sibship blue or green with fresh water of sea water is close, and growth course is identical.The biology that fresh water is blue or green
, nutritional need and full gene order-checking have been studied clear the most, and it is available for sea water green grass or young crops reference for information about,
Same experiment can also be done with fresh water green grass or young crops.
This experiment sea water Mylopharyngodon piceus kind is given by City University of Hong Kong's friendship, at this laboratory breeding three
More than Dai, can be that experiment provides sufficient experiment prelarva quantity.This room has been accumulated in controllable experimental ecosystem
In system, successfully hatch, cultivate and study the sea water green grass or young crops reply physiology of Acid stress, pathology toxicity and regulation and control
Experience and experiment skill.
Being utilized respectively a-f product and feed fish, (salinity 30 ± 2, water temperature is 28 ± 2 DEG C to condition of breeding fish, pH
It is 8.10, dissolved oxygen amount >=6.10mg/L, Light To Dark Ratio is 14h:10h) the most identical with experimental implementation.Every day three
Secondary 200 mesh feedstuffs of throwing something and feeding, total feeding volume is the 5% of fish body weight.Observe in detail every day and record prelarva movable,
Ingest, lopsided, dead etc., random acquisition fish sample after 6 weeks, weigh respectively, be stored in-80 DEG C of refrigerators
For molecule, gene, physiology and biochemical measurement.Result is as follows:
The signal factor GPx gene relative expression quantity (2 of sea water prelarva-△△Ct) respectively 2.18,
2.31、2.86、2.91、2.4、1.10。
The signal factor PPAR α gene relative expression quantity (2 of sea water prelarva-△△Ct) respectively 1.83,
1.95、2.11、2.20、1.94、0.98。
Rate of body weight gain (BWG%) is respectively 84.01,85.94,87.21,88.54,86.05,81.12.
Specific growth rate (SGR%) is respectively 4.38,4.51,4.65,4.73,4.43,4.24.
Malonaldehyde (MDA) content is respectively 1.23,1.21,1.18,1.15,1.21,1.34nmol/mgprot.
GPx content (active) be respectively 130.36,138.68,149.35,135.95,77.13U/mgprot.
CAT content (active) be respectively 2.85,2.94,3.29,3.20,2.88,2.41U/mgprot.
SOD content (active) be respectively 10.79,11.95,13.96,13.92,10.95,8.91U/mgprot.
T-AOC content (active) be respectively 1.19,1.23,1.39,1.32,1.25,1.04U/mg.
Sum it up, as the addition Cortex Eucommiae powder 25-75g of each component, shell widow in every kilogram of basic dry feed
Sugar 3-12g, tea tree oil 0.05-0.15g, ALA-GLN 2.5-7.5g, vitamin E 0.01-0.05g,
When citric acid 3-9g, selenium 0.0002-0.001g, the rate of body weight gain (BWG%) of fish, specific growth rate (SGR%),
GPx, CAT, SOD and T-AOC content or activity are the highest, and malondialdehyde
(MDA) minimum, illustrate that feedstuff of the present invention can be effectively improved Fish oxidation resistance, promote generation
Thank, alleviate the Acid stress impact on Fish.
Although embodiment of the present invention are disclosed as above, but it is not restricted to description and embodiment party
Listed utilization in formula.It can be applied to various applicable the field of the invention completely.For being familiar with ability
For the personnel in territory, can easily revise him and use.Therefore limited without departing substantially from claim and equivalency range
Under fixed general concept, the present invention is not limited to specific details and shown here as the example with description.
Claims (8)
1. regulate and control fish sea water acidifying stress a feedstuff, wherein, including normal feedstuff part and additive
Part；
Wherein said normal feedstuff part mainly includes following components: protein sources material 780-820, Semen sojae atricolor phosphorus
Fat 9-11, starch 60-90, fish oil 45-55, mineral mixture 13-17 and vitamin mixtures 13-17；
Described additive part mainly includes following components: Cortex Eucommiae powder 25-75, oligochitosan 3-12, tea tree oil
0.05-0.15, ALA-GLN 2.5-10, vitamin E 0.01-0.05.
2. regulation and control fish sea water acidifying as claimed in claim 1 stress feedstuff, wherein, described additive
Part also includes the one in following component or several: citric acid 3-12, selenium 0.0002-0.001.
3. regulation and control fish sea water acidifying as claimed in claim 2 stress feedstuff, wherein, described protein sources
Material mainly comprises the one in following component or several: Erythroculter ilishaeformis powder 550-570, casein 40-55 and
Semen sojae atricolor 160-210.
4. regulation and control fish sea water acidifying as claimed in claim 3 stress feedstuff, wherein, described vitamin
Mixture comprises following component: vitamin A 60-100, vitamin D330-50, vitamin K3 0.1-0.15、
Vitamin B10.1-0.2, vitamin B20.2-0.4, vitamin B60.2-0.4, vitamin B12 0.001-0.003、
Nicotinic acid 0.01-0.02, Potassium pantothenate. 0.5-0.8, folic acid 0.02-0.05, biotin 0.001-0.003, inositol
1-3, vitamin C 0.08-0.12 and choline chloride 8-12；
Described mineral mixture comprises following component: ferrous sulfate 120-200, zinc sulfate 500-700,
Manganese chloride 30-50, copper sulfate 150-260, iodine 8-12, magnesium chloride 150-260, cobaltous sulfate 15-25 and molybdenum
Acid sodium 15-25.
5. regulation and control fish sea water acidifying as claimed in claim 4 stress feedstuff, wherein, every kilogram dry raises
Material mainly includes following components:
Erythroculter ilishaeformis powder 560g, casein 50g, Semen sojae atricolor 160g, soybean phospholipid 9g, starch 75g, fish oil 50g,
Mineral mixture 15g, vitamin mixtures 15g, Cortex Eucommiae powder 50g, oligochitosan 6g, tea tree oil 0.05g,
ALA-GLN 5g, vitamin E 0.02g, citric acid 6g, selenium powder 0.0009g.
6. regulation and control fish sea water acidifying as claimed in claim 4 stress feedstuff, wherein, every kilogram dry raises
Material mainly includes following components:
Erythroculter ilishaeformis powder 556g, casein 45g, Semen sojae atricolor 180g, soybean phospholipid 10g, starch 85g, fish oil
45g, mineral mixture 14g, vitamin mixtures 14g, Cortex Eucommiae powder 25g, oligochitosan 12g, Camellia sinensis
Oil 0.1g, ALA-GLN 7.5g, vitamin E 0.03g, citric acid 9g, selenium powder 0.0006g.
7. regulation and control fish sea water acidifying as claimed in claim 4 stress feedstuff, wherein, every kilogram dry raises
Material mainly includes following components:
Erythroculter ilishaeformis powder 551g, casein 40g, Semen sojae atricolor 170g, soybean phospholipid 11g, starch 70g, fish oil
48g, mineral mixture 13g, vitamin mixtures 13g, Cortex Eucommiae powder 75g, oligochitosan 3g, Camellia sinensis
Oil 0.15g, ALA-GLN 3g, vitamin E 0.04g, citric acid 3g, selenium powder 0.0003g.
8. regulation and control fish sea water acidifying as claimed in claim 2 stress feedstuff, wherein, described oligochitosan
Molecular weight be 500-550, the degree of polymerization is 4-5；Described selenium be particle diameter be the nanometer selenium of 500-600nm.
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| CN108094761A (en) * | 2018-01-04 | 2018-06-01 | 广东海洋大学 | A kind of feed addictive for promoting the field planting of egg-shaped pompano intestinal beneficial microbes |
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Application publication date: 20160928 |