CN102940157A - Traditional Chinese medicine feed additive for improving disease resistance of grass carp - Google Patents
Traditional Chinese medicine feed additive for improving disease resistance of grass carp Download PDFInfo
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Abstract
The invention provides a feed for improving the disease resistance of a grass carp. The feed is characterized by comprising soybean cake, wheat meal, shell meal, fish meal, table salt, rice bran, fish oil, betaine, traditional Chinese medicine feed premix additive, zinc-containing polypeptide, zinc-containing microelement additive, vitamin mixture additive at least containing vitamin C, vitamin E and inositol, yeast and amino acid mixture additive at least containing threonine. The feed provided by the invention can improve the immunity of the organism of the grass carp and protect the grass carp from getting sick; the application of antibacterial chemical medicines and anti-virus chemical medicines is reduced to a certain degree, the survival rate of the grass carp is increased, and the activity of lysozyme and superoxide dismutase in the grass carp is improved; and moreover, the feed ensures little drug residue and low toxicity, and does not influence the quality of the grass carp or harm the human health.
Description
Technical field
The present invention relates to technical field of aquaculture, relate in particular to a kind of Chinese herbal feed additive for improving the grass carp resistance against diseases.
Background technology
Grass carp (
Ctenopharyngodon idellus), belong to Cyprinidae, Leuciscinae, grass carp genus, be commonly called as careless grass carp, grass roots (northeast), one of China famous " four large Chinese carps ", deeply being subjected to people's favor, is that people like best edible a kind of marine product, and the grass carp aquaculture is the mariculture industry of China's maximum.
But in four large Chinese carps, grass carp is the most liable to illness, mainly be to suffer from enteritis, red skin etc., brought very large difficulty to the grass carp cultivation, in addition, hemorrhage also be grass carp be very easy to suffer from by a kind of communicable disease of exhaling intestines guanidine virus to cause, in breeding process, this disease of easy infection all from the fingerling to the adult fish, this disease incidence of disease is high, the death rate is very large, causes great economic loss for cultivation specialist every year.
In order to treat the various diseases of grass carp, the raiser uses antibiotic and chemicals in a large number, cause in animal body residual, impact and worsen product quality, the serious harm health.
In order to improve the level of production of grass carp aquaculture, prevent that disease from bringing the tremendous economic loss of grass carp aquaculture, should be contained from the source, by improving specific immunity ability and the non-specific immunity of grass carp, be resisted and prophylactic ability thereby improve it.
That Chinese medicine has is natural, efficient, toxic and side effect is little, the resistance to the action of a drug not significantly, the advantage such as aboundresources and performance be various, it has unique effect aspect prevent disease, manyly studies confirm that multiple Chinese herbal medicine all can affect aquatic livestock vivo oxidation enzyme activity, phagocyte vigor and Bacteriolytic activity, thus the enhanced machine body immunity function.
Chinese herbal medicine contains the materials such as a large amount of flavones, alkaloid, organic acid and immune polysaccharide, can improve Immune Function In Animals, promotes interferon, lysozyme and the content of superoxide dismutase in serum, is the sign of animal disease resistant diseases prevention ability.Use Chinese herbal medicine can reduce the use of chemical classes medicine, there are explicit stipulation a lot of chemical classes medicines of inaccurate use in aquaculture of aquatic animal countries in the world recently, widely popularize organic cultivation, in aquaculture, with Chinese herbal medicine instead of chemical synthetic such as chloramphenicol etc., will have very wide prospect.
Summary of the invention
Technical problem to be solved by this invention provides a kind of Chinese herbal feed additive for raising grass carp resistance against diseases and the feed that contains this feed addictive, by this feed of feeding, can improve the immunity of organisms of grass carp, prevent that grass carp is ill, reduce to a certain extent the use of antibacterium chemical drugs and antiviral chemical drugs, improve the grass carp survival rate, improve the vigor of the interior lysozyme of grass carp body and superoxide dismutase, and this feed medicament residue rate is low, toxicity is low, can not affect the quality of grass carp, can not be detrimental to health.
In order to solve the problems of the technologies described above, the invention provides a kind of feed for improving the grass carp resistance against diseases, it comprises soya-bean cake, wheat flour, oyster shell whiting, fish meal, salt, rice bran, fish oil, betaine, Chinese medicine fodder premix material additive, contains the zinc polypeptide, the iron content trace mineral supplement, at least contain the vitamin mixtures additive, yeast of vitamin C, vitamin E and inositol, contain the ispol additive of threonine at least.
Wherein, in the described feed percentage by weight of each component be soya-bean cake 5% ~ 9%, wheat flour 30% ~ 36%, oyster shell whiting 3% ~ 6%, fish meal 4% ~ 7%, salt 1% ~ 2%, rice bran 13% ~ 18%, fish oil 2% ~ 5%, betaine 1.5% ~ 2.5%, Chinese medicine fodder premix material additive 15% ~ 18%, contain zinc polypeptide 0.1% ~ 0.3%, iron content trace mineral supplement 0.2% ~ 0.3%, at least contain the vitamin mixtures additive 2% ~ 5%, yeast 2% ~ 4% of vitamin C, vitamin E and inositol, contain the ispol additive 2% ~ 5% of threonine at least.
Wherein, in the described feed percentage by weight of each component be soya-bean cake 7%, wheat flour 33%, oyster shell whiting 4.1%, fish meal 5%, salt 1.5%, rice bran 16%, fish oil 3%, betaine 2%, Chinese medicine fodder premix material additive 17%, contain zinc polypeptide 0.2%, iron content trace mineral supplement 0.2%, at least contain the vitamin mixtures additive 4%, yeast 3% of vitamin C, vitamin E and inositol, contain the ispol additive 4% of threonine at least.
Wherein, described Chinese herbal feed additive comprises following bulk drug: motherwort, holly leaf, tinosporae, prepared rhizome of rehmannia, dark plum, granatum, Herba Andrographitis, azedarach, dendrobium candidum, the Radix Astragali, scorpio, the vine of multiflower knotweed, Kaempferia galanga, the fruit of glossy privet, radix semiaquilegiae, arillus longan, folium cortex eucommiae.
Wherein, the weight portion of each bulk drug is motherwort 21 weight portions ~ 33 weight portions in the described Chinese herbal feed additive, holly leaf 26 weight portions ~ 39 weight portions, tinosporae 17 weight portions ~ 24 weight portions, prepared rhizome of rehmannia 39 weight portions ~ 52 weight portions, dark plum 10 weight portions ~ 18 weight portions, granatum 13 weight portions ~ 22 weight portions, Herba Andrographitis 20 weight portions ~ 28 weight portions, azedarach 16 weight portions ~ 24 weight portions, dendrobium candidum 37 weight portions ~ 55 weight portions, the Radix Astragali 79 weight portions ~ 88 weight portions, scorpio 30 weight portions ~ 36 weight portions, the vine of multiflower knotweed 47 weight portions ~ 58 weight portions, Kaempferia galanga 11 weight portions ~ 19 weight portions, the fruit of glossy privet 49 weight portions ~ 61 weight portions, radix semiaquilegiae 15 weight portions ~ 22 weight portions, arillus longan 38 weight portions ~ 46 weight portions, folium cortex eucommiae 46 weight portions ~ 53 weight portions.
Wherein, the weight portion of each bulk drug is motherwort 27 weight portions, holly leaf 32 weight portions, tinosporae 21 weight portions, prepared rhizome of rehmannia 45 weight portions, dark plum 14 weight portions, granatum 18 weight portions, Herba Andrographitis 24 weight portions, azedarach 20 weight portions, dendrobium candidum 46 weight portions, the Radix Astragali 84 weight portions, scorpio 33 weight portions, the vine of multiflower knotweed 53 weight portions, Kaempferia galanga 15 weight portions, the fruit of glossy privet 55 weight portions, radix semiaquilegiae 19 weight portions, arillus longan 42 weight portions, folium cortex eucommiae 50 weight portions in the described Chinese herbal feed additive.
The present invention also provides the preparation method of above-mentioned feed, and it is specially:
The first step, each bulk drug component in the above-mentioned Chinese herbal feed additive is ground to form respectively fine powder, mix, obtain mixture, mixture is joined in the distilled water of 6 ~ 8 times of amounts and decoct, decocted 2 ~ 4 hours at every turn, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, it is in 90% ~ 95% the ethanolic solution that medicinal extract is joined determining alcohol with respect to 3 ~ 5 times of amounts of mixture quality, stir fully dissolving, 4 ℃ of following standing over night of temperature, subsequent filtration carries out reduced pressure concentration with filtrate and removes ethanol subsequently, the lotion that obtains is pulverized, and adding starch according to the ratio of mass ratio 1:3 ~ 5, mixing is granulated with 14 mesh sieves, baking is 30 minutes in 60 ℃ ~ 70 ℃ baking ovens, obtains Chinese herbal feed additive;
Second step, it is even that the Chinese herbal feed additive that the first step is obtained and other each components in the described feed are sent into stirrer for mixing in proportion, sends in the pulverizer subsequently and pulverize, and crosses 80～100 purpose screen clothes;
The 3rd step; material after pulverizing is sent in the modem; pass into the steam that accounts for quality of material 15%～20%; 90～100 ℃ temperature modulated 6～7 minutes; then the modulation products that obtains is entered the extrusion granulator under 95～100 ℃ temperature and granulate, under 90～103 ℃ of conditions, the oven dry of the feed granules after expanded is got final product.
The present invention also provides the Chinese herbal feed additive that is used for improving the grass carp resistance against diseases, and it comprises following bulk drug: motherwort, holly leaf, tinosporae, prepared rhizome of rehmannia, dark plum, granatum, Herba Andrographitis, azedarach, dendrobium candidum, the Radix Astragali, scorpio, the vine of multiflower knotweed, Kaempferia galanga, the fruit of glossy privet, radix semiaquilegiae, arillus longan, folium cortex eucommiae.
Wherein, the parts by weight of described each bulk drug are motherwort 21 weight portions ~ 33 weight portions, holly leaf 26 weight portions ~ 39 weight portions, tinosporae 17 weight portions ~ 24 weight portions, prepared rhizome of rehmannia 39 weight portions ~ 52 weight portions, dark plum 10 weight portions ~ 18 weight portions, granatum 13 weight portions ~ 22 weight portions, Herba Andrographitis 20 weight portions ~ 28 weight portions, azedarach 16 weight portions ~ 24 weight portions, dendrobium candidum 37 weight portions ~ 55 weight portions, the Radix Astragali 79 weight portions ~ 88 weight portions, scorpio 30 weight portions ~ 36 weight portions, the vine of multiflower knotweed 47 weight portions ~ 58 weight portions, Kaempferia galanga 11 weight portions ~ 19 weight portions, the fruit of glossy privet 49 weight portions ~ 61 weight portions, radix semiaquilegiae 15 weight portions ~ 22 weight portions, arillus longan 38 weight portions ~ 46 weight portions, folium cortex eucommiae 46 weight portions ~ 53 weight portions.
The present invention also provides the preparation method of above-mentioned Chinese herbal feed additive, and it is specially:
Above-mentioned raw materials medicine component is ground to form respectively fine powder, mix, obtain mixture, mixture is joined in the distilled water of 6 ~ 8 times of amounts and decoct, the each decoction 2 ~ 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, it is in 90% ~ 95% the ethanolic solution that medicinal extract is joined determining alcohol with respect to 3 ~ 5 times of amounts of mixture quality, stirs fully dissolving, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out reduced pressure concentration remove ethanol, the lotion of acquisition is pulverized, and adds starch according to the ratio of mass ratio 1:3 ~ 5, mixing, granulate with 14 mesh sieves, baking is 30 minutes in 60 ℃ ~ 70 ℃ baking ovens, obtains Chinese herbal feed additive.
Beneficial effect of the present invention:
[0019]This feed provided by the invention, can improve the immunity of organisms of grass carp, prevent that grass carp is ill, reduce to a certain extent the use of antibacterium chemical drugs and antiviral chemical drugs, improve the grass carp survival rate, improve the vigor of the interior lysozyme of grass carp body and superoxide dismutase, and this feed medicament residue rate is low, toxicity is low, can not affect the quality of grass carp, can not be detrimental to health.
The specific embodiment
The invention provides a kind of feed for improving the grass carp resistance against diseases, it comprises soya-bean cake, wheat flour, oyster shell whiting, fish meal, salt, rice bran, fish oil, betaine, Chinese medicine fodder premix material additive, contains the zinc polypeptide, the iron content trace mineral supplement, at least contain the vitamin mixtures additive, yeast of vitamin C, vitamin E and inositol, contain the ispol additive of threonine at least.
The percentage by weight of each component is soya-bean cake 5% ~ 9% in the described feed, wheat flour 30% ~ 36%, oyster shell whiting 3% ~ 6%, fish meal 4% ~ 7%, salt 1% ~ 2%, rice bran 13% ~ 18%, fish oil 2% ~ 5%, betaine 1.5% ~ 2.5%, Chinese medicine fodder premix material additive 15% ~ 18%, contain zinc polypeptide 0.1% ~ 0.3%, iron content trace mineral supplement 0.2% ~ 0.3%, at least contain vitamin C, the vitamin mixtures additive 2% ~ 5% of vitamin E and inositol, yeast 2% ~ 4%, at least the ispol additive 2% ~ 5% that contains threonine, the mass percent sum of each component is 100% in the described feed.
In the described feed percentage by weight of each component most preferably be soya-bean cake 7%, wheat flour 33%, oyster shell whiting 4.1%, fish meal 5%, salt 1.5%, rice bran 16%, fish oil 3%, betaine 2%, Chinese medicine fodder premix material additive 17%, contain zinc polypeptide 0.2%, iron content trace mineral supplement 0.2%, at least contain the vitamin mixtures additive 4%, yeast 3% of vitamin C, vitamin E and inositol, contain the ispol additive 4% of threonine at least.
Preferred described feed only is made of said components.
Betaine has obvious metabolic activity, can improve the survival rate of grass carp, and simultaneously, the food calling power of betaine and palatability can attract grass carp to go to ingest.
Zinc is the necessary trace elements of most of biologies, has important physiological function; It is that many enzymes are (such as carbonic anhydrase, carboxypeptidase, alkaline phosphatase, lactic dehydrogenase etc.) constituent or activator, in the grass carp body, zinc participates in multiple metabolic process, comprise carbohydrate, lipid, synthetic and the degraded of protein and nucleic acid, scarce zinc can make immune organ such as the thymus gland of animal, lymph node, atrophy occurs in spleens etc., periphery lymphocyte reduces, the T cell function weakens, thereby cause body immunity to reduce, replenishing zinc then makes its functional recovery normal, adopting among the present invention that to contain the zinc polypeptide be Bacitracin Zinc premix, is the pale yellow powder shape crystal that Tianjin nova animal pharmaceutical factory produces.
Iron is a kind of hematopoiesis element, it is the important composition composition of hemoglobin, iron has important function to immunity of organism mechanism, because iron is activated centre or the coenzyme of some enzyme in nucleic acid reductase and the biological oxidation process (xanthine oxidase, succinate dehydrogenase etc.), iron deficiency can affect the activity of these enzymes, thereby affect the synthetic and T Proliferation of lymphocytes of DNA, periphery lymph T lymphocyte in blood quantity is reduced, affect thick liquid cell synthesizing immunoglobulin (Ig), make the phagocyte activity impaired, cause lethality to reduce.Thereby the leucocyte sterilizing ability reduces during iron deficiency, body is easily infected, metainfective hematoclasis accelerates, the body death rate raises, if the utilization of iron content deficiency or iron affects adversely in the feed, then may cause fish growth to slow down, premunition descends, and the iron content trace mineral supplement that adds among the present invention is ironic citrate.
Vitamin C is one of fish and the necessary nutritional labeling of shrimps, and, mineral absorption synthetic with hormone, metabolism have substantial connection, and can strengthen immunity of organisms, prevention scurvy, protection capilary, promotes wound healing.
Described vitamin E is the important nutriment of aquatic livestock; its major function is to eliminate unnecessary free radical in the body; protect fat-soluble cell membrane and unrighted acid not oxidized, improve the immunity of animal body by the function of promotion and opsonic immunity cell.
Vitamin C and vitamin E lay respectively at endochylema and the phospholipid bilayer of cell as important antioxidant, for cell provides effective antioxidant system.The former can remove normal cell activity and the various free radicals that stress descend to produce; the latter is protecting highly unsaturated fatty acid to avoid playing an important role in the peroxidating of free radical mediated; therefore, an amount of vitamin C and vitamin E can improve the immunity of aquatic animal, anti-oxidant and anti-stress ability.
Inositol can make the speed of growth of grass carp accelerate, and simultaneously, reduces the generation of grass carp disease.
Described vitamin mixtures additive also can be selected any vitamin premix additive that comprises vitamin C, vitamin E and inositol or add the vitamin premix additive when adding vitamin C, vitamin E, described vitamin premix additive can be FV-102 vitamin compound premix (Zhongmu Industry Co.,Ltd's production), and it contains vitamin C is that 100g/kg, vitamin E are 50,000 IU/kg.
Threonine is the first limiting amino acids that IgG generates, and threonine lacks can Immunosuppression globulin and the lymphocytic generation of T, B.
Described ispol additive also can add other amino acid additives such as methionine, serine, lysine.
Described Chinese herbal feed additive comprises following bulk drug: motherwort, holly leaf, tinosporae, prepared rhizome of rehmannia, dark plum, granatum, Herba Andrographitis, azedarach, dendrobium candidum, the Radix Astragali, scorpio, the vine of multiflower knotweed, Kaempferia galanga, the fruit of glossy privet, radix semiaquilegiae, arillus longan, folium cortex eucommiae.
Described Chinese herbal feed additive preferably only is made of the above-mentioned raw materials medicine.
The weight portion of each bulk drug can be motherwort 21 weight portions ~ 33 weight portions in the described Chinese herbal feed additive, holly leaf 26 weight portions ~ 39 weight portions, tinosporae 17 weight portions ~ 24 weight portions, prepared rhizome of rehmannia 39 weight portions ~ 52 weight portions, dark plum 10 weight portions ~ 18 weight portions, granatum 13 weight portions ~ 22 weight portions, Herba Andrographitis 20 weight portions ~ 28 weight portions, azedarach 16 weight portions ~ 24 weight portions, dendrobium candidum 37 weight portions ~ 55 weight portions, the Radix Astragali 79 weight portions ~ 88 weight portions, scorpio 30 weight portions ~ 36 weight portions, the vine of multiflower knotweed 47 weight portions ~ 58 weight portions, Kaempferia galanga 11 weight portions ~ 19 weight portions, the fruit of glossy privet 49 weight portions ~ 61 weight portions, radix semiaquilegiae 15 weight portions ~ 22 weight portions, arillus longan 38 weight portions ~ 46 weight portions, folium cortex eucommiae 46 weight portions ~ 53 weight portions.
The weight portion of each bulk drug most preferably is motherwort 27 weight portions in the described Chinese herbal feed additive, holly leaf 32 weight portions, tinosporae 21 weight portions, prepared rhizome of rehmannia 45 weight portions, dark plum 14 weight portions, granatum 18 weight portions, Herba Andrographitis 24 weight portions, azedarach 20 weight portions, dendrobium candidum 46 weight portions, the Radix Astragali 84 weight portions, scorpio 33 weight portions, the vine of multiflower knotweed 53 weight portions, Kaempferia galanga 15 weight portions, the fruit of glossy privet 55 weight portions, radix semiaquilegiae 19 weight portions, arillus longan 42 weight portions, folium cortex eucommiae 50 weight portions.
Azedarach can be removed the grass carp vivotoxin, regulate metabolic function, prevents and treats early the generation of various parasites, bacteriosis and spreads.
Astragalus polyose in the Radix Astragali can obviously promote the generation of body antibody, can make the serum IgA of grass carp, the level rise of IgM, IgG, antibody secreting cell and T, B lymphocyte and NK cell obviously strengthen, and strengthen normal grass carp and immunosupress grass carp peritoneal macrophage number and function.
Chinese herbal feed additive of the present invention can stimulate grass carp body linked groups to produce lysozyme and the superoxide dismutase of higher vigor, lysozyme is the important nospecific immunity factors of many organisms, being the important component part of grass carp disease system of defense, is to finish the material base of engulfing.Superoxide dismutase is one of important antioxidase in the living organism Antioxidant Enzyme Systems, as the active oxygen radical scavenger, is delaying body aging and is preventing from playing a very important role aspect the large biological molecule damage; Also has antiviral and antibacterial action, Chinese herbal feed additive of the present invention is by stimulating grass carp to produce lysozyme and the superoxide dismutase of higher vigor, thereby improve specific immunity ability and the non-specific immunity of grass carp, and then improve the anti-virus ability of grass carp.
The present invention also provides the preparation method of above-mentioned Chinese herbal feed additive, and it is specially:
Above-mentioned Chinese medicine material medicine component is ground to form respectively fine powder, mix, obtain mixture, mixture is joined in the distilled water of 6 ~ 8 times of amounts and decoct, the each decoction 2 ~ 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, it is in 90% ~ 95% the ethanolic solution that medicinal extract is joined determining alcohol with respect to 3 ~ 5 times of amounts of mixture quality, stirs fully dissolving, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out reduced pressure concentration remove ethanol, the lotion of acquisition is pulverized, and adds starch according to the ratio of mass ratio 1:3 ~ 5, mixing, granulate with 14 mesh sieves, baking is 30 minutes in 60 ℃ ~ 70 ℃ baking ovens, obtains Chinese herbal feed additive.
The present invention also provides the preparation method of above-mentioned feed, and it is specially:
The first step prepares described Chinese herbal feed additive according to the method described above;
Second step, it is even that the Chinese herbal feed additive that the first step is obtained and other each components in the described feed are sent into stirrer for mixing in proportion, sends in the pulverizer subsequently and pulverize, and crosses 80～100 purpose screen clothes;
The 3rd step; material after pulverizing is sent in the modem; pass into the steam that accounts for quality of material 15%～20%; 90～100 ℃ temperature modulated 6～7 minutes; then the modulation products that obtains is entered the extrusion granulator under 95～100 ℃ temperature and granulate, under 90～103 ℃ of conditions, the oven dry of the feed granules after expanded is got final product.
Below adopt embodiment to describe embodiments of the present invention in detail, how the application technology means solve technical problem to the present invention whereby, and the implementation procedure of reaching technique effect can fully understand and implements according to this.
Embodiment 1 Chinese herbal feed additive 1
With motherwort 27g, holly leaf 32g, tinosporae 21g, prepared rhizome of rehmannia 45g, dark plum 14g, granatum 18g, Herba Andrographitis 24g, azedarach 20g, dendrobium candidum 46g, Radix Astragali 84g, scorpio 33g, vine of multiflower knotweed 53g, Kaempferia galanga 15g, fruit of glossy privet 55g, radix semiaquilegiae 19g, arillus longan 42g, folium cortex eucommiae 50g grinds to form respectively fine powder, mix, obtain mixture, mixture is joined in the distilled water of 4kg and decoct, the each decoction 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, the determining alcohol that medicinal extract is joined 3kg is in 95% the ethanolic solution, to stir fully dissolving, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out reduced pressure concentration remove ethanol, the lotion of acquisition is pulverized, and adds 3kg starch, mixing, granulate with 14 mesh sieves, baking is 30 minutes in 70 ℃ of baking ovens, obtains Chinese herbal feed additive 1.
Embodiment 2 Chinese herbal feed additives 2
With motherwort 33g, holly leaf 39g, tinosporae 24g, prepared rhizome of rehmannia 52g, dark plum 18g, granatum 22g, Herba Andrographitis 28g, azedarach 16 24g, dendrobium candidum 55g, Radix Astragali 88g, scorpio 36g, vine of multiflower knotweed 58g, Kaempferia galanga 19g, fruit of glossy privet 61g, radix semiaquilegiae 22g, arillus longan 46g, folium cortex eucommiae 53g grinds to form respectively fine powder, mix, obtain mixture, mixture is joined in the distilled water of 5kg and decoct, the each decoction 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, the determining alcohol that medicinal extract is joined 3.5kg is in 95% the ethanolic solution, to stir fully dissolving, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out reduced pressure concentration remove ethanol, the lotion of acquisition is pulverized, and adds 3kg starch, mixing, granulate with 14 mesh sieves, baking is 30 minutes in 70 ℃ of baking ovens, obtains Chinese herbal feed additive 2.
Embodiment 3 Chinese herbal feed additives 3
With motherwort 21g, holly leaf 26g, tinosporae 17g, prepared rhizome of rehmannia 39g, dark plum 10g, granatum 13g, Herba Andrographitis 20g, azedarach 16g, dendrobium candidum 37g, Radix Astragali 79g, scorpio 30g, vine of multiflower knotweed 47g, Kaempferia galanga 11g, fruit of glossy privet 49g, radix semiaquilegiae 15g, arillus longan 38g, folium cortex eucommiae 46g grinds to form respectively fine powder, mix, obtain mixture, mixture is joined in the distilled water of 3.5kg and decoct, the each decoction 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, the determining alcohol that medicinal extract is joined 2.5kg is in 95% the ethanolic solution, to stir fully dissolving, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out reduced pressure concentration remove ethanol, the lotion of acquisition is pulverized, and adds 3kg starch, mixing, granulate with 14 mesh sieves, baking is 30 minutes in 70 ℃ of baking ovens, obtains Chinese herbal feed additive 3.
Toxicity test
Test with the initial body weight 58.75 ± 3.49g of two grass carps in age, physical health, specification is consistent.
Feeding and management
Test is raised at glass jar (in 0.54m * 0.26m * 0.45m) with fish.Raised and train grass carp 7 with basal feed before on-test, to conform and test feed.After on-test, except the feed of normally throwing something and feeding every day, 8:00 begins to fill with the Chinese herbal feed additive 1mL/ tail of hello the present invention preparation, every day 1 time.Glass jar 24 is uninterruptedly inflated.Water temperature is 22 ℃～24 ℃ during the cultivation, and the pH value is 7.6 ± 0.5, and dissolved oxygen is 6.7～7.4mg/L.
Test method
In first trial test, 210 tail grass carps are divided into 7 groups at random, establish 3 repetitions for every group, each repeats 10 tails, the fish body weight is without significant difference (P＞0.05) between group, 5 groups of Chinese herbal feed additives 1 that use the present invention's preparation wherein, it is dissolved in the distilled water uses, determine 5 concentration (2.5g/mL with 10 times difference, 0.25g/ml, 0.025/ml, 0.0025g/ml, 0.00025g/ml), 96h is processed in test, through test of many times, and progressively dwindles concentration range, find out at last 100% lethasl concentration (g/ml) and maximal tolerable concentration (g/ml), and according to etc. contrast spacing method determine each test group concentration (concrete grammar: r=n-1
R is adjacent two dose ratio in the formula; B is maximum dose level; A is lowest dose level; N is dosage group number), other two groups of Chinese herbal feed additive 2 and Chinese herbal feed additives 3 that use respectively the embodiment of the invention 2 and embodiment 3 preparations are dissolved in it in distilled water and use, and concentration is 2.5g/mL.
Computational methods
Adopt the karber method to calculate half lethal concentration LC
50Computing formula LgLC
50=Xm-d (∑ p-0.5)
Xm is the common logarithm of dead group maximum dose in the formula; D is that adjacent set concentration common logarithm is poor; P is the death rate of each group; ∑ p is the summation of each group death rate.
The safe concentration computing formula
Sc＝?48hLC
50×0.3／(24hLC
50／48hLC
50)
2
Experimental result sees Table 1.
Table 1 Chinese herbal feed additive is to the death rate, half lethal concentration and the safe concentration of grass carp
From table 1 can, adopting Chinese herbal feed additive of the present invention is 0 at 24h and the equal death rate of 96h, can't calculate its half lethal concentration and safe concentration according to formula, but 24hLC
50Necessarily greater than 2.5g/mL, thereby can find out, Chinese herbal feed additive of the present invention is a kind of green non-poisonous feed addictive.
Embodiment 4 feeds 1
With 170g Chinese herbal feed additive 1, the 70g soya-bean cake, the 330g wheat flour, 41g oyster shell whiting (production of Rongcheng, Shandong fish meal Co., Ltd), 50g fish meal (production of Rongcheng, Shandong fish meal Co., Ltd), 15g salt, the 160g rice bran, 30g fish oil, 20g betaine (production of the high chemical industry of Shandong moral Co., Ltd), 2g Bacitracin Zinc premix (the pale yellow powder shape crystal that nova animal pharmaceutical factory in Tianjin produces), 2g ironic citrate (production of power sky, Jinan food additives Co., Ltd), 40gFV-102 vitamin compound premix (Zhongmu Industry Co.,Ltd's production), 30g yeast (production of power sky, Jinan food additives Co., Ltd), 20g threonine (the grand day amino acid additive Co., Ltd in Jinan produces), 20g lysine (the grand day amino acid additive Co., Ltd in Jinan produces), send in the pulverizer subsequently and pulverize, cross 100 purpose screen clothes; Material after pulverizing is sent in the modem; pass into the steam that accounts for quality of material 18%; 100 ℃ temperature modulated 7 minutes; then the modulation products that obtains being entered the extrusion granulator under 100 ℃ temperature granulates; under 100 ℃ of conditions, the oven dry of the feed granules after expanded is got final product, obtain feed 1.
Embodiment 5 feeds 2
With 170g Chinese herbal feed additive 2, the 70g soya-bean cake, the 330g wheat flour, 41g oyster shell whiting (production of Rongcheng, Shandong fish meal Co., Ltd), 50g fish meal (production of Rongcheng, Shandong fish meal Co., Ltd), 15g salt, the 160g rice bran, 30g fish oil, 20g betaine (production of the high chemical industry of Shandong moral Co., Ltd), 2g Bacitracin Zinc premix (the pale yellow powder shape crystal that nova animal pharmaceutical factory in Tianjin produces), 2g ironic citrate (production of power sky, Jinan food additives Co., Ltd), 40gFV-102 vitamin compound premix (Zhongmu Industry Co.,Ltd's production), 30g yeast (production of power sky, Jinan food additives Co., Ltd), 20g threonine (the grand day amino acid additive Co., Ltd in Jinan produces), 20g lysine (the grand day amino acid additive Co., Ltd in Jinan produces), send in the pulverizer subsequently and pulverize, cross 100 purpose screen clothes; Material after pulverizing is sent in the modem; pass into the steam that accounts for quality of material 18%; 100 ℃ temperature modulated 7 minutes; then the modulation products that obtains being entered the extrusion granulator under 100 ℃ temperature granulates; under 100 ℃ of conditions, the oven dry of the feed granules after expanded is got final product, obtain feed 2.
Embodiment 6 feeds 3
With 170g Chinese herbal feed additive 3, the 70g soya-bean cake, the 330g wheat flour, 41g oyster shell whiting (production of Rongcheng, Shandong fish meal Co., Ltd), 50g fish meal (production of Rongcheng, Shandong fish meal Co., Ltd), 15g salt, the 160g rice bran, 30g fish oil, 20g betaine (production of the high chemical industry of Shandong moral Co., Ltd), 2g Bacitracin Zinc premix (the pale yellow powder shape crystal that nova animal pharmaceutical factory in Tianjin produces), 2g ironic citrate (production of power sky, Jinan food additives Co., Ltd), 40gFV-102 vitamin compound premix (Zhongmu Industry Co.,Ltd's production), 30g yeast (production of power sky, Jinan food additives Co., Ltd), 20g threonine (the grand day amino acid additive Co., Ltd in Jinan produces), 20g lysine (the grand day amino acid additive Co., Ltd in Jinan produces), send in the pulverizer subsequently and pulverize, cross 100 purpose screen clothes; Material after pulverizing is sent in the modem; pass into the steam that accounts for quality of material 18%; 100 ℃ temperature modulated 7 minutes; then the modulation products that obtains being entered the extrusion granulator under 100 ℃ temperature granulates; under 100 ℃ of conditions, the oven dry of the feed granules after expanded is got final product, obtain feed 3.
Experiment
Animal used as test: test is juvenile fish with grass carp, scale of construction 79g ~ 85g/ tail (3600 tail), and healthy strong, male and female all have, and are purchased from the fishing ground, Jinan.
The experiment feed
The feed 1 of experimental group one to the three difference feeding embodiment of the invention 4 to embodiment 6 preparations is to feed 3, the feed of control group feeding and the difference of experimental group are, use the Chinese herbal feed additive 1 of flavomycoin 20mg/kg replacement the present invention use to Chinese herbal feed additive 3, every group contains juvenile fish 900 tails.
Feeding method
Be 7 days～August 12 March in 2010 date of test.Juvenile fish is tested after raising and train 7 d indoor.The pond crucian carp fish culture in the pond, stocking density 1/10 L.Pool area 900 m
2(long 42 m * wide 22 m), dark 80 cm, underneath fine sand 10 cm, with bamboo close (water can pass through, fish can not by being standard) all separation block into 15 m
2Xiao Chi; Omnidistance average 16 ℃ of pond water temperature (wherein summer 24～29 ℃, average 25.5 ℃), pH value 6.5 ± 0.2, water quality meet the fishery standard.
Feed day, feeding volume weighed 3%～5% quantitative of fixed place and time throwing something and feeding by fry, and the 2 times/d that throws something and feeds is every 10 d dosages 1 time.The survival rate of investigation grass carp.
Fish is put to death, with buffer solution (pH value 7.5,0.25mol/ sucrose, 0.025mol/L Tris-HCL, 0.1mmol/L EDTA-2Na solution) flushing, suck surface moisture with blotting paper again, remove the sclerotin part, stay the tissue homogenates such as heart, liver and sexual gland.Accurately take by weighing tissue 0.30 g, add 9 times buffer solution ice bath homogenate, the centrifugal 10～15min of 3 000 r/m in low-temperature and high-speed centrifuges gets supernatant 2 mL and measures lysozyme and superoxide dismutase vigor.Assay method builds up the kit specification that biological study is produced with reference to Nanjing.
Test data adopts means standard deviation, and (x ± s) expression, two sample means are relatively with t check, two sample constituent ratio χ
2Check, there is conspicuousness P＜0.05 for difference.
The result
Chinese herbal feed additive sees Table 2 to the impact of grass carp survival rate.
Table 2 Chinese herbal feed additive is on the impact of grass carp survival rate
As can be seen from Table 2, adopt the feed feeding grass carp of the present invention's preparation, its ill possibility reduces, and survival rate is significantly higher than control group.
Chinese herbal feed additive the results are shown in Table 3 to the impact of lysozyme and superoxide dismutase vigor.
Table 3 Chinese herbal feed additive is on the impact of lysozyme and superoxide dismutase vigor
As can be seen from Table 3, adopt the interior lysozyme of grass carp body and the superoxide dismutase level of Chinese herbal feed additive feeding of the present invention to significantly improve, thereby prove that it has better defence disease, antiviral and antibacterial ability, immune level is higher.
Each bulk drug pharmacology of described Chinese medicine composition is as follows:
Motherwort: bitter, suffering, cold nature enters liver, pericardium channel, has promoting blood circulation for regulating menstruation, the effect of inducing diuresis to reduce edema.Be used for irregular menstruation, dysmenorrhoea is through closing lochiorrhagia, oedema oliguria; Acute nephritis edema, motherwort have cardiac stimulant, increase the effect of coronary flow and myocardial nutritional flow amount. and motherwort has slight excitation to the rabbit heart in place; Extrasomatic rabbit heart then there is first slight inhibition, slight excited later on, but heavy dose then suppresses. its stachydrine reducing heart rate. and leonurine has humidification to the convergent force low dose of isolated frog heart, and heavy dose then presents inhibition.This kind inhibition may be relevant with excited vagus.Experimental results show that: motherwort has remarkable increase coronary flow and the quite significantly effect of reducing heart rate.Motherwort 1ml/kg is to the anesthetized dog intravenous injection, the increase coronary flow also being arranged, reduce coronary resistance, reducing heart rate and reduce the effect of cardiac output and the work done of left chamber. motherwort all has in various degree antagonism to animal models such as expeirmental myocardial ischemia, myocardial infarction or arrhythmia cordis.Motherwort the myocardial ischemia in rats of Induced By Isoprenaline is improved or recover the ischemic cardiac electrograph, increase coronary flow, improve microcirculation, dog myocardial infarction that rabbit myocardial ischemia that the effect such as reducing heart rate, motherwort formulation are brought out pituitrin and crown artery ligation cause; can make that its lesion degree alleviates, infarction size reduces, myocardial ultrastructure is protected.
Holly leaf: bitter, cool in nature, return liver, kidney channel, have the effect of clearing heat and nourishing yin, the flat liver of kidney-nourishing, cure mainly the tuberculosis hemoptysis, osteopyrexia and fever, the card such as have a dizzy spell.
Tinosporae: bitter, cold in nature, return lung, large intestine channel, have clearing heat and detoxicatingly, the effect of relieving sore throat and acesodyne is used for abscess of throat, and the ulcer serious case of furuncle is had loose bowels, dysentery, card such as gastral cavity abdomen heat pain grade.
Prepared rhizome of rehmannia: distinguish the flavor of sweet, warm in nature, return liver, kidney channel, have enrich blood moist, the effect that the benefit essence is filled out marrow is used for the deficiency of blood sallow, dizzy palpitaition, irregular menstruation, collapse incessantly liver-kidney Yin deficiency, hectic fever night sweat, seminal emission impotence, infertility, irregular menstruation, metrostaxis, soreness and weakness of waist and knees, Hiccough and deaf, the head is dim-sighted, poliosis, quench one's thirst constipation, the cards such as syndrome characterized by dyspnea of suffering from a deficiency of the kidney.
Dark plum: bitter, acid, puckery, warm in nature, enter liver, lung, spleen, large intestine channel, have and astringe the lung, puckery intestines, hemostasis, the effect of promoting the production of body fluid is used for the treatment of protracted dysentery more than, damp-heat dysentery is had blood in stool, the card such as hematuria, snowslide, and is inhibited to multiple germs such as shigella dysenteriae, Escherichia coli, typhoid bacilluses.
Granatum: flavor acid, puckery, warm in nature, enter large intestine, kidney channel, have relieving diarrhea with astringents, the effect of hemostasis desinsection is used for rushing down protracted dysentery for a long time, hematochezia, under the uterine bleeding band, the cards such as abdominal pain due to enterositosis.
Herba Andrographitis: bitter, cold in nature, the thoughts of returning home, lung, large intestine, urinary bladder channel, have clearing heat and detoxicating, cool blood, detumescence, the effect of eliminating dampness cures mainly cold, fever, abscess of throat, aphthae, pertussis labor is coughed, dysentery, heat is drenched puckery pain, the carbuncle sore that swells, the cards such as venomous snake bite.
Azedarach: latin name
Meliae Cortex, bitter, cold in nature, return liver, spleen, stomach warp, have desinsection, treat tinea, be used for roundworm disease, enterobiasis, the cards such as abdominal pain due to enterositosis.
Dendrobium candidum: latin name
Dendorbii Officinalis Caulis, to distinguish the flavor of sweet, cold nature has the nourishing the stomach of promoting the production of body fluid; Nourishing Yin and clearing heat; The moistening lung kidney-nourishing; The make eye bright effect of strong waist cures mainly pyreticosis Tianjin and hinders, the dry polydipsia, and deficiency of stomach-Yin, food is few retches, and abnormal heat is not moved back after being ill, and is fire excess from yin deficiency, hectic fever due to yin labor heat, order is secretly not clear, and muscles and bones is withered soft, Compendium of Material Medica: except gas under the numbness, the tonifying five zang organs consumptive disease is won thin, reinforcing yin essence benefit essence.Take for a long time, thick stomach, never sufficient in the benefit, flat stomach Qi, longue meat by skin heat symptoms caused by an exopathgen miliaria gas, a little less than the painful cold numbness of pin knee, is decided intelligence except frightened, makes light of one's life by commiting suicide and prolongs life.Benefit gas heat extraction, strong sun, cold for a long time in the bone by the skin wandering arthritis, kidney tonifying benefit power.Smelting heating spontaneous perspiration, plug in the ulcer apocenosis.
The Radix Astragali: distinguish the flavor of sweet, warm in nature, enter spleen, lung channel, have help spleen soil, elevate a turnable ladder yang-energy, strengthening exterior and reducing sweat, the effect of holder sore myogenic cures mainly the weak anorexia and loose stool due to spleen-lung Qi deficiency, palpitation, the visceral organ ptosis of the sinking of qi of middle-jiao, Qi dysfunction in blood control uterine bleeding is had blood in stool, and rushes down for a long time prolapse of the anus; The spontaneous sweating of exterior deficiency, the cards such as insufficiency of vital energy and blood.
Scorpio: flavor is hot, and property is flat, returns Liver Channel, has breath wind antispastic, dispersing pathogen accumulation, and the effect of removing obstruction in channels to relieve pain cures mainly child convulsion, the tic spasm, middle air port is askew, hemiplegia, lockjaw, rheumatoid arthritis stubborn, migraine and general headache, sore, the cards such as scrofula.
The vine of multiflower knotweed: distinguish the flavor of sweet, property is flat, and the thoughts of returning home, Liver Channel have nourishing blood and tranquilization, and the effect of dispelling wind and removing obstruction in the meridians cures mainly insomnia and dreamful sleep, deficiency of blood bodily pain, arthralgia due to wind-dampness; The cards such as pruitus, the vine of multiflower knotweed can be invigorated blood circulation, stimulated the menstrual flow, hemangiectasis, stimulate circulation, the lactogenesis detumescence.
Kaempferia galanga: flavor is hot, warm in nature, returns the stomach warp, has in the promoting the circulation of qi temperature, and the effect of the pain relieving that helps digestion cures mainly chest diaphragm turgor, coldness and pain in the epigastrium, the diet card such as do not disappear.
The fruit of glossy privet: distinguish the flavor of sweet, bitter, be slightly cold, enter liver, kidney channel, strong liver kidney-nourishing is arranged, the effect of removing heat to brighten vision is mainly used in treating liver-kidney Yin deficiency, and the head loses foster dizzy tinnitus, the symptoms such as yin asthenia generating intrinsic heat.The fruit of glossy privet can promote leucocyte, strengthens the effect of immunologic function, and staphylococcus aureus, shigella dysenteriae are had stronger inhibitory action.Modern medicine study proves, the fruit of glossy privet includes ursolic acid, sweet mellow wine, glucose, fatty wet goods, and cardiac stimulant, diuresis and hepatoprotective effect are arranged, and the leucocyte decline quantity that causes because of chemotherapy raise, and staphylococcus aureus, Escherichia coli are had inhibitory action.
Radix semiaquilegiae: distinguish the flavor of sweet, little hardship, little suffering, cold in nature, return liver, spleen, urinary bladder channel, have clearing heat and detoxicating; Dispersing swelling and dissipating binds; The effect of promoting diuresis for stranguria.Cure mainly children's's heat frightened; Epilepsy; Carbuncle is swollen; Furunculosis; Acute mastitis; Scrofula; The skin sore of itching; Red eye, swell pain; Pharyngalgia; Snake bite and insect sting; Heat is drenched; The cards such as sand pouring.
Arillus longan: warm in nature, distinguish the flavor of sweet, have tonifying heart and spleen, the effect of nourishing blood and tranquilization is used for the cards such as insufficiency of vital energy and blood, palpitation and severe palpitation, forgetful insomnia, the deficiency of blood be sallow.
Folium cortex eucommiae: latin name
Eucommiae Folium, little suffering of distinguishing the flavor of, warm in nature, return liver, kidney channel, have filling liver kidney, strengthening the bones and muscles cures mainly kidney deficiency and liver, has a dizzy spell waist knee ache, the soft card that waits of muscles and bones impotence.
This intellectual property of primary enforcement that all are above-mentioned is not set restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above only is preferred embodiment of the present invention, is not to be the restriction of the present invention being made other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not break away to any simple modification, equivalent variations and remodeling that above embodiment does, still belongs to the protection domain of technical solution of the present invention according to technical spirit of the present invention.
Claims (10)
1. be used for improving the feed of grass carp resistance against diseases, it is characterized in that, comprising: soya-bean cake, wheat flour, oyster shell whiting, fish meal, salt, rice bran, fish oil, betaine, Chinese medicine fodder premix material additive, contain zinc polypeptide, iron content trace mineral supplement, at least contain the vitamin mixtures additive, yeast of vitamin C, vitamin E and inositol, contain the ispol additive of threonine at least.
2. feed as claimed in claim 1, it is characterized in that: the percentage by weight of each component is soya-bean cake 5% ~ 9% in the described feed, wheat flour 30% ~ 36%, oyster shell whiting 3% ~ 6%, fish meal 4% ~ 7%, salt 1% ~ 2%, rice bran 13% ~ 18%, fish oil 2% ~ 5%, betaine 1.5% ~ 2.5%, Chinese medicine fodder premix material additive 15% ~ 18%, contain zinc polypeptide 0.1% ~ 0.3%, iron content trace mineral supplement 0.2% ~ 0.3%, at least contain vitamin C, the vitamin mixtures additive 2% ~ 5% of vitamin E and inositol, yeast 2% ~ 4%, at least the ispol additive 2% ~ 5% that contains threonine.
3. feed as claimed in claim 1 or 2 is characterized in that: in the described feed percentage by weight of each component be soya-bean cake 7%, wheat flour 33%, oyster shell whiting 4.1%, fish meal 5%, salt 1.5%, rice bran 16%, fish oil 3%, betaine 2%, Chinese medicine fodder premix material additive 17%, contain zinc polypeptide 0.2%, iron content trace mineral supplement 0.2%, at least contain the vitamin mixtures additive 4%, yeast 3% of vitamin C, vitamin E and inositol, contain the ispol additive 4% of threonine at least.
4. such as the described feed of claims 1 to 3, it is characterized in that: described Chinese herbal feed additive comprises following bulk drug: motherwort, holly leaf, tinosporae, prepared rhizome of rehmannia, dark plum, granatum, Herba Andrographitis, azedarach, dendrobium candidum, the Radix Astragali, scorpio, the vine of multiflower knotweed, Kaempferia galanga, the fruit of glossy privet, radix semiaquilegiae, arillus longan, folium cortex eucommiae.
5. such as the described feed of claim 1 to 4, it is characterized in that: the weight portion of each bulk drug is motherwort 21 weight portions ~ 33 weight portions in the described Chinese herbal feed additive, holly leaf 26 weight portions ~ 39 weight portions, tinosporae 17 weight portions ~ 24 weight portions, prepared rhizome of rehmannia 39 weight portions ~ 52 weight portions, dark plum 10 weight portions ~ 18 weight portions, granatum 13 weight portions ~ 22 weight portions, Herba Andrographitis 20 weight portions ~ 28 weight portions, azedarach 16 weight portions ~ 24 weight portions, dendrobium candidum 37 weight portions ~ 55 weight portions, the Radix Astragali 79 weight portions ~ 88 weight portions, scorpio 30 weight portions ~ 36 weight portions, the vine of multiflower knotweed 47 weight portions ~ 58 weight portions, Kaempferia galanga 11 weight portions ~ 19 weight portions, the fruit of glossy privet 49 weight portions ~ 61 weight portions, radix semiaquilegiae 15 weight portions ~ 22 weight portions, arillus longan 38 weight portions ~ 46 weight portions, folium cortex eucommiae 46 weight portions ~ 53 weight portions.
6. such as the described feed of claim 1 to 5, it is characterized in that: the weight portion of each bulk drug is motherwort 27 weight portions, holly leaf 32 weight portions, tinosporae 21 weight portions, prepared rhizome of rehmannia 45 weight portions, dark plum 14 weight portions, granatum 18 weight portions, Herba Andrographitis 24 weight portions, azedarach 20 weight portions, dendrobium candidum 46 weight portions, the Radix Astragali 84 weight portions, scorpio 33 weight portions, the vine of multiflower knotweed 53 weight portions, Kaempferia galanga 15 weight portions, the fruit of glossy privet 55 weight portions, radix semiaquilegiae 19 weight portions, arillus longan 42 weight portions, folium cortex eucommiae 50 weight portions in the described Chinese herbal feed additive.
7. the preparation method of the described feed of claim 1 to 6 is characterized in that, is specially:
The first step, each bulk drug component in the above-mentioned Chinese herbal feed additive is ground to form respectively fine powder, mix, obtain mixture, mixture is joined in the distilled water of 6 ~ 8 times of amounts and decoct, decocted 2 ~ 4 hours at every turn, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, it is in 90% ~ 95% the ethanolic solution that medicinal extract is joined determining alcohol with respect to 3 ~ 5 times of amounts of mixture quality, stir fully dissolving, 4 ℃ of following standing over night of temperature, subsequent filtration carries out reduced pressure concentration with filtrate and removes ethanol subsequently, the lotion that obtains is pulverized, and adding starch according to the ratio of mass ratio 1:3 ~ 5, mixing is granulated with 14 mesh sieves, baking is 30 minutes in 60 ℃ ~ 70 ℃ baking ovens, obtains Chinese herbal feed additive;
Second step, it is even that the Chinese herbal feed additive that the first step is obtained and other each components in the described feed are sent into stirrer for mixing in proportion, sends in the pulverizer subsequently and pulverize, and crosses 80～100 purpose screen clothes;
The 3rd step; material after pulverizing is sent in the modem; pass into the steam that accounts for quality of material 15%～20%; 90～100 ℃ temperature modulated 6～7 minutes; then the modulation products that obtains is entered the extrusion granulator under 95～100 ℃ temperature and granulate, under 90～103 ℃ of conditions, the oven dry of the feed granules after expanded is got final product.
8. be used for improving the Chinese herbal feed additive of grass carp resistance against diseases, it is characterized in that, comprise following bulk drug: motherwort, holly leaf, tinosporae, prepared rhizome of rehmannia, dark plum, granatum, Herba Andrographitis, azedarach, dendrobium candidum, the Radix Astragali, scorpio, the vine of multiflower knotweed, Kaempferia galanga, the fruit of glossy privet, radix semiaquilegiae, arillus longan, folium cortex eucommiae.
9. Chinese herbal feed additive as claimed in claim 8, it is characterized in that: the parts by weight of described each bulk drug are motherwort 21 weight portions ~ 33 weight portions, holly leaf 26 weight portions ~ 39 weight portions, tinosporae 17 weight portions ~ 24 weight portions, prepared rhizome of rehmannia 39 weight portions ~ 52 weight portions, dark plum 10 weight portions ~ 18 weight portions, granatum 13 weight portions ~ 22 weight portions, Herba Andrographitis 20 weight portions ~ 28 weight portions, azedarach 16 weight portions ~ 24 weight portions, dendrobium candidum 37 weight portions ~ 55 weight portions, the Radix Astragali 79 weight portions ~ 88 weight portions, scorpio 30 weight portions ~ 36 weight portions, the vine of multiflower knotweed 47 weight portions ~ 58 weight portions, Kaempferia galanga 11 weight portions ~ 19 weight portions, the fruit of glossy privet 49 weight portions ~ 61 weight portions, radix semiaquilegiae 15 weight portions ~ 22 weight portions, arillus longan 38 weight portions ~ 46 weight portions, folium cortex eucommiae 46 weight portions ~ 53 weight portions.
10. the preparation method of claim 8 or 9 described Chinese herbal feed additives is characterized in that, is specially:
Above-mentioned raw materials medicine component is ground to form respectively fine powder, mix, obtain mixture, mixture is joined in the distilled water of 6 ~ 8 times of amounts and decoct, the each decoction 2 ~ 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out reduced pressure concentration and obtain medicinal extract, it is in 90% ~ 95% the ethanolic solution that medicinal extract is joined determining alcohol with respect to 3 ~ 5 times of amounts of mixture quality, stirs fully dissolving, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out reduced pressure concentration remove ethanol, the lotion of acquisition is pulverized, and adds starch according to the ratio of mass ratio 1:3 ~ 5, mixing, granulate with 14 mesh sieves, baking is 30 minutes in 60 ℃ ~ 70 ℃ baking ovens, obtains Chinese herbal feed additive.
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Effective date of registration: 20170612 Address after: 438000, No. three nanhu road, Nanhu Nanhu Industrial Park, Huanggang, Hubei, Huanggang, 9 Patentee after: Huanggang new hope feed Technology Co., Ltd. Address before: 266000 Shandong city of Qingdao province Chengyang Jihongtan Street Green Industrial Park Patentee before: Shandong New Hope Liuhe Group Co., Ltd. |
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