CN105060497B - A kind of compound oxygenation agent and preparation method for removing cultivation water nitrite - Google Patents

A kind of compound oxygenation agent and preparation method for removing cultivation water nitrite Download PDF

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CN105060497B
CN105060497B CN201510420857.3A CN201510420857A CN105060497B CN 105060497 B CN105060497 B CN 105060497B CN 201510420857 A CN201510420857 A CN 201510420857A CN 105060497 B CN105060497 B CN 105060497B
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牛晓君
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South China University of Technology SCUT
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Abstract

The invention belongs to technical field of aquaculture, a kind of compound oxygenation agent and preparation method for removing cultivation water nitrite is disclosed.The oxygenation agent is prepared from by the component of following weight portion:40~50 parts of oxygenation composition, 24~38 parts of complex micro organism fungicide, 5~29 parts of adsorbent, 1.0~2.5 parts of growth promoter, 7~10 parts of Chinese medicine additive, 0.02~0.12 part of synergist, 1~3 part of stabilizer, 1.4~2.2 parts of sustained release agent;Described complex micro organism fungicide includes denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, bacillus laterosporus, bacillus subtilis, red city Rhodococcus sp, silicate bacteria, streptococcus fecalis and bacillus coagulans.The compound multiple-microorganism microbial inoculum of oxygenation agent of the invention, can effectively remove nitrite, and with suppressing breeding water body pathogen and improving aquatic livestock enteron aisle digestive function, with preferable application value.

Description

A kind of compound oxygenation agent and preparation method for removing cultivation water nitrite
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of compound oxygenation of removal cultivation water nitrite Agent and preparation method.
Background technology
In the last few years, aquaculture quickly grows, especially in Freshwater cultivation, mostly pond or batch production construction, Itself it is closing, water body is chronically at static state, influence of the oxygen content and content of nitrite in water body to water body environment It is very big, the yield of aquaculture is directly connected to, especially with the development of high-density aquiculture, substantial amounts of fishes and shrimps excretion The organic depositions such as thing, residual bait are water-bed, and oxide layer is thinning in making bottom of pond top layer, and the reducing zone of lower floor is thickening, redox electricity Potential drop is low.In the cultivation busy season, because temperature is high, cause heterotrophic microorganism amount reproduction, organic matter to be decomposed, discharge substantial amounts of The harmful substances such as ammonia, nitrite, so as to influence the growth of fishes and shrimps, development, trigger fishes and shrimps disease, and organic in breeding water body Self-purification capacity of the load beyond original flora, it is therefore desirable to artificial to add water body purification preparation to improve water body environment.
Nitrite is important water quality physical and chemical index in aquaculture, and content of nitrite is raised, and not only illustrates pond Bottom anoxic, cultivation water deteriorates, while being also the inducement of aquatic animal prone disease.When the nitrite concentration accumulation in water To after 0.1mg/L, be mainly for fish, shrimp, the crab generation harm to being cultivated in water body, its mechanism of action by nitrite:Nitrous acid The infiltration and absorption of the gill and body surface that salt passes through aquatic livestock, into the oxygen carrying protein binding in blood, with blood, and are allowed to lose The function of carrying oxygen is gone, so as to show as hypoxic conditions.Even if the dissolved oxygen in water body is high, aquatic livestock also shows falls vacant Oxygen, comatose state, such as food ration decline, expiratory dyspnea, move about slow, flag, and gill portion be damaged blackening, appearance " the trip pool ", The phenomenon such as " floating head ", " dead steathily ", " emitting bottom ".In addition, aquatic livestock is lived in the higher water body of nitrite for a long time, Food ration reduction, vigor is poor, and autoimmunity declines, easy pathogenic infection, and fulminant disease.
Water nitrite is controlled to have been achieved for preferable effect, but technology at present using the method for biological treatment In, water quality is controlled using single microorganism strain mostly, degradation efficiency is not high enough, there is limitation, therefore, various differences are micro- Bacteria agent carries out optimal fit scale combination, using the interaction between them, influences each other to play its maximum colony Advantage, in the hope of reaching the purpose of efficient fast degradation, also problem and study hotspot as cultural technique.And existing water purification agent Unitary function, does not reach the requirement of high-density aquiculture, therefore develops a kind of multi-functional water body purification preparation and have pole Big meaning.
The content of the invention
In order to solve the shortcoming and defect part of above prior art, primary and foremost purpose of the invention is to provide a kind of removal The compound oxygenation agent of cultivation water nitrite.
Another object of the present invention is to provide a kind of system of the compound oxygenation agent of above-mentioned removal cultivation water nitrite Preparation Method.
The object of the invention is achieved through the following technical solutions:
A kind of compound oxygenation agent for removing cultivation water nitrite, is prepared from by the component of following weight portion:
40~50 parts of oxygenation composition, 24~38 parts of complex micro organism fungicide, 5~29 parts of adsorbent, growth promoter 1.0~ 2.5 parts, 7~10 parts of Chinese medicine additive, 0.02~0.12 part of synergist, 1~3 part of stabilizer, 1.4~2.2 parts of sustained release agent;
Described complex micro organism fungicide includes denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, side spore gemma Bacillus, bacillus subtilis, red city Rhodococcus sp, silicate bacteria, streptococcus fecalis and bacillus coagulans.
Denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, side spore gemma bar in the complex micro organism fungicide Bacterium, bacillus subtilis, red city Rhodococcus sp, the weight between silicate bacteria, streptococcus fecalis and bacillus coagulans are than preferred It is 3:1:2:2:3:1:1:0.5:1.
Described oxygenation composition includes the weight of percarbamide and coated sodium peracrbonate, percarbamide and coated sodium peracrbonate Than being preferably 1:(3~5).
Described adsorbent is made up of medical stone, atlapulgite, zeolite powder, white carbon, superfine silica gel powder and calcium phosphate;Institute State the weight ratio preferably 4 between medical stone, atlapulgite, zeolite powder, white carbon, superfine silica gel powder and calcium phosphate:2:1:1: 0.5:0.3。
Described growth promoter is made up of Sodium Pyruvate, arsanilic acid, potassium chloride and calcium sulfate;The acetone Sour sodium, the weight ratio preferably 1 between arsanilic acid, potassium chloride and calcium sulfate:1:2:2.
Described Chinese medicine additive is made up of calamitas urinae hominis, Rhizoma Trillii Tschonoskii, kohlrabi leaf, barley bubble, pine needle and Semen Setariae italicae;Each group Weight/mass percentage composition of the active ingredient divided in Chinese medicine additive is preferably:Calamitas urinae hominis 12%, Rhizoma Trillii Tschonoskii 15%, kohlrabi leaf 26%th, barley bubble 7%, Semen Setariae italicae 20%, pine needle 14%, folium artemisiae argyi 6%.
The preferred sodium perchlorate of described synergist.
The preferred silicate of described stabilizer or phosphate;More preferably sodium metasilicate, magnesium silicate or nitrilo trimethylene phosphorus Acid.
The preferred sodium alginate of described sustained release agent or polyvinyl alcohol.
The preparation method of the compound oxygenation agent of above-mentioned removal cultivation water nitrite, including following preparation process:
By denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, bacillus laterosporus, bacillus subtilis, Hong Cheng Rhodococcus sp, silicate bacteria, streptococcus fecalis, the bacterial strain of bacillus coagulans are respectively adopted high density liquid fermentation, then to fermentation Product is extracted and refined, and is made hyperconcetration bacterium powder, and various bacterium powders are mixed, and obtains complex micro organism fungicide;By oxygenation into Divide, complex micro organism fungicide mixes with adsorbent, add growth promoter, Chinese medicine additive, synergist, stabilizer and sustained release Agent, granulates after being well mixed, and obtains removing the compound oxygenation agent of cultivation water nitrite.
Described hyperconcetration bacterium powder refers to that spore content reaches 1012~1013The bacterium powder of CFU/g.
Described oxygenation composition is prepared by the following method:Percarbamide and coated sodium peracrbonate are weighed, mixer is placed on It is interior, the lubricant relative to percarbamide and coated sodium peracrbonate gross weight 8%~25% is added, mix 5~20 with mixer Minute, obtain oxygenation composition.The preferred polyoxyethylene monostearate of described lubricant or adipic acid.
Described adsorbent is prepared by the following method:Weigh medical stone, atlapulgite, zeolite powder, white carbon, micro mist silicon Glue and calcium phosphate, are crushed respectively, are crushed to grain fineness for 0.3~1.2mm, are mixed to get mixed-powder, then will be mixed Close powder to be processed through inorganic acidifying, calcined, cooled down, adsorbent is obtained.
Described inorganic acidifying treatment refers to by 1 with 1%~6% dilute sulfuric acid with the mixed-powder:0.8 mass ratio 30~60h of acidification at ambient temperature;Described calcining refers to be calcined under conditions of temperature is for 300~340 DEG C.
Described Chinese medicine additive is prepared by the following method:
By calamitas urinae hominis with 25~35 DEG C of 5~10min of water process, 40~50 DEG C of 12~20min for the treatment of in baking oven are placed in, put Fried in frying pan to burned black, pulverization after cooling, obtain calamitas urinae hominis's powder;
By the dry rhizome portion of Rhizoma Trillii Tschonoskii with 25~35 DEG C of 0.5~1min of water process, dry, be ground into carefully with pulverizer Powder, 120 mesh sieving, obtains Rhizoma Trillii Tschonoskii dry powder;
Kohlrabi leaf is cleaned up, after drying, sesame oil is added in frying pan, heated, treat that sesame oil temperature reaches 200~250 DEG C when, kohlrabi leaf is put into 3~5min is fried in sesame oil, kohlrabi leaf is drained, pull out, ground after cooling, obtain kohlrabi leaf powder;
Barley bubble root skin is taken, is cleaned up, dried, be ground into meal, be placed in boiler and add water to cook extraction 1~3 time, often Secondary 30~60min, filtering, merging filtrate is condensed into medicinal extract, then is spray-dried and obtains barley bubble extract powder, by filter residue and drying, powder It is broken, 100~120 mesh sieves are crossed, merge with barley bubble extract powder, that is, obtain barley bubble powder;
Semen Setariae italicae is cleaned, 80~120 DEG C of 10~20min of baking are placed in baking oven, taken out, be placed in frying pan and fry to ripe Thoroughly, cool down, pulverization obtains Semen Setariae italicae fine powder;
Pine needle edible tender branch and needle are taken, is cleaned up, dried naturally, crushed with pulverizer, the sieving of 80~120 mesh is obtained To pine needle meal;
Folium artemisiae argyi is cleaned, is placed in boiler and is added water to cook extraction, the water for being added is 15~18 times of folium artemisiae argyi weight, 100~110 DEG C of 40~60min of decoction, filtering, filtrate concentrates and is spray-dried and obtains Folium Artemisiae Argyi extract powder;
By above-mentioned calamitas urinae hominis's powder, Rhizoma Trillii Tschonoskii dry powder, kohlrabi leaf powder, barley bubble powder, Semen Setariae italicae fine powder, pine needle meal and Chinese mugwort Leaf extract powder presses following weight/mass percentage composition:It is calamitas urinae hominis's powder 12%, Rhizoma Trillii Tschonoskii dry powder 15%, kohlrabi leaf powder 26%, big Wheat bubble powder 7%, Semen Setariae italicae fine powder 20%, pine needle meal 14%, Folium Artemisiae Argyi extract powder 6% are mixed, and obtain Chinese medicine additive.
Described granulation refers to be pressed into the particle that granularity is 5~20 millimeters with forcing press.
Preparation method of the invention and resulting product have the following advantages that and beneficial effect:
(1) oxygenation agent oxygenation of the invention is rapid, long-acting, can be effectively improved breeding water body anoxic conditions;
(2) the compound multiple-microorganism microbial inoculum of oxygenation agent of the invention, can effectively remove nitrite, and degradation speed Hurry up, pollution is few, and can suppress breeding water body pathogen, prevents disease from occurring, improve cultivation survival rate, also with antisepsis and Improve aquatic livestock enteron aisle digestive function, promote feed digestion to absorb, improve the yield of aquatic livestock;
(3) oxygenation agent of the invention also contains natural minerals adsorbent, can not only adsorb harmful substance, purifies water, also Containing various trace elements necessary to aquatic livestock, mineral nutrition is supplemented;
(4) oxygenation agent of the invention adds various medicinal herb componentses, helps food digestion to absorb, and improves efficiency of feed utilization, And rich in the multiple nutritional components and active material for promoting aquatic livestock to grow, enhance immunity;
(5) component of different efficacies can be carried out by good combination by preparation method of the invention so that each component Function can give full play to;
(6) safely and effectively, non-poisonous material pollutes, high financial profit, with preferable for preparation method of the invention and products therefrom Application value.
Brief description of the drawings
Fig. 1 is 3 parts of changes of water sample day part dissolved oxygen amount after being separately added into the compound oxygenation agent of the embodiment of the present invention 1~3 Curve map;
Fig. 2 is 3 parts of changes of water sample day part ammonia-nitrogen content after being separately added into the compound oxygenation agent of the embodiment of the present invention 1~3 Change curve map;
Fig. 3 is 3 parts of water sample day part content of nitrite after being separately added into the compound oxygenation agent of the embodiment of the present invention 1~3 Change curve.
Specific embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited In this.
Bacterium source in the complex micro organism fungicide of following examples is as follows:
Denitrifying bacteria is aerobic denitrifying bacteria, specially Paracoccus denitrificans, and bacterium numbering is 10489, latin name: Paracoccusdenitrificans, purchased from American Type Culture Collecti;
The culture presevation numbering of trichoderma harzianum is the latin names of CCAM 041228:Trichoderma harzianum, purchase From Hua Zhong Agriculture University, agricultural and utilizes center at microorganism germ plasma and genetic resources preservation;
The culture presevation numbering of bacillus amyloliquefaciens is 10149, latin name:Bacillus Amyloliquefaciens, purchased from Chinese agriculture Microbiological Culture Collection administrative center;
The culture presevation numbering of bacillus laterosporus is 05440, latin name:Bacillus laterosporus, in being purchased from Agricultural Microbiological Culture Collection administrative center of state;
The culture presevation numbering of bacillus subtilis is 01914, latin name:Bacillus subtilis, purchased from Chinese agriculture Industry Microbiological Culture Collection administrative center;
The culture presevation numbering of red city Rhodococcus sp is 40320, latin name:Rhodococcuserythropolis, in being purchased from Agricultural Microbiological Culture Collection administrative center of state;
The silicate bacteria specifically refers to gel-shaped bacillus, and culture presevation numbering is 11005, latin name: Bacillus mucilaginosus, purchased from Forestry Inst., Chinese Forestry Science Academy;
The culture presevation numbering of streptococcus fecalis is 10180, latin name:Streptococcus faecalis, purchased from China Agriculture Microbiological Culture Collection administrative center;
Bacillus coagulans culture presevation numbering is 00397, latin name:Bacillus coagulans, purchased from Chinese agriculture Industry Microbiological Culture Collection administrative center.
Embodiment 1
A kind of compound oxygenation agent of removal cultivation water nitrite of the present embodiment, is prepared by the component of following weight portion Form:
40 parts of oxygenation composition, 24 parts of complex micro organism fungicide, 5 parts of adsorbent, 1.0 parts of growth promoter (are by weight ratio 1:1:2:2 Sodium Pyruvate, arsanilic acid, potassium chloride and calcium sulfate composition), 7 parts of Chinese medicine additive, sodium perchlorate 0.02 Part, 1 part of sodium metasilicate, 1.4 parts of sodium alginate;
Its specific preparation method is as follows:
(1) oxygenation composition treatment:By weight ratio it is 1:3 weigh percarbamide and coated sodium peracrbonate, are placed on mixer It is interior, the polyoxyethylene monostearate relative to percarbamide and coated sodium peracrbonate gross weight 8% is added, mixed with mixer 5 minutes, it was dry mixed-powder to be well mixed, and obtains oxygenation composition;
(2) complex micro organism fungicide is prepared:By denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, side spore gemma Bacillus, bacillus subtilis, red city Rhodococcus sp, silicate bacteria, streptococcus fecalis, the bacterial strain of bacillus coagulans are respectively adopted height Density fluid is fermented, then tunning is extracted and refined, and is made hyperconcetration bacterium powder, and the gemma of the hyperconcetration bacterium powder contains Amount reaches 1012CFU/g, by denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, bacillus laterosporus, bacillus subtilis Bacterium, red city Rhodococcus sp, silicate bacteria, streptococcus fecalis, the part by weight of bacillus coagulans are 3:1:2:2:3:1:1:0.5:1 Above-mentioned various bacterium powders are mixed, complex micro organism fungicide is obtained, it is stand-by;
(3) adsorbent is prepared:By weight ratio it is 4:2:1:1:0.5:0.3 weigh medical stone, atlapulgite, zeolite powder, White carbon, superfine silica gel powder, calcium phosphate, are crushed respectively, are crushed to grain fineness for 0.3mm, and mixing obtains mixed-powder, Through with 1% dilute sulfuric acid 1 is pressed with the mixed-powder:0.8 ratio acidification 30h at ambient temperature, in 300 DEG C of temperature Under the conditions of calcine 1h, cool down, be obtained adsorbent, it is stand-by;
(4) Chinese medicine additive is prepared:
A. calamitas urinae hominis's treatment:By calamitas urinae hominis with 25 DEG C of water process 5min, 40 DEG C for the treatment of 12min in baking oven are placed in, be placed in Fried in frying pan to burned black, pulverization after cooling, obtain calamitas urinae hominis's powder, it is stand-by;
B. Rhizoma Trillii Tschonoskii treatment:By the dry rhizome portion of Rhizoma Trillii Tschonoskii with 25 DEG C of water process 0.5min, dry, use pulverizer powder Fine powder is broken into, 120 mesh sieve repeatedly, obtains Rhizoma Trillii Tschonoskii dry powder, it is stand-by;
C. kohlrabi leaf treatment:Kohlrabi leaf is cleaned up, after drying, sesame oil is added in frying pan, heated, treat sesame oil temperature When reaching 200 DEG C, kohlrabi leaf is put into sesame oil and fries 3min, kohlrabi leaf is drained, pulled out, ground after cooling, obtain kohlrabi leaf Powder, it is stand-by;
D. barley bubble treatment:Barley bubble root skin is taken, is cleaned up, dried, be ground into meal, be placed in boiler and add water to cook Extract 1 time, each 30min, filtering, merging filtrate is condensed into medicinal extract, then be spray-dried and obtain barley bubble extract powder, by filter residue Dry, pulverize, cross 100 mesh sieves, merge with barley bubble extract powder, that is, obtain barley bubble powder, it is stand-by;
E. Semen Setariae italicae treatment:Semen Setariae italicae is cleaned, 80 DEG C of baking 10min are placed in baking oven, taken out, be placed in frying pan fry to It is well-done, cooling, pulverization obtains Semen Setariae italicae fine powder, stand-by;
F. pine needle treatment:Pine needle edible tender branch and needle are taken, is cleaned up, dried naturally, crushed with pulverizer, 80 mesh mistakes Sieve, obtains pine needle meal, stand-by;
G. folium artemisiae argyi treatment:Folium artemisiae argyi is cleaned, is placed in boiler and is added water to cook extraction, the water for being added is folium artemisiae argyi weight 15 times, 100 DEG C of decoction 40min, filtering, filtrate concentrates and is spray-dried and obtains Folium Artemisiae Argyi extract powder, stand-by;
By above-mentioned calamitas urinae hominis's powder, Rhizoma Trillii Tschonoskii dry powder, kohlrabi leaf powder, barley bubble powder, Semen Setariae italicae fine powder, pine needle meal and Chinese mugwort Leaf extract powder presses following weight/mass percentage composition:It is calamitas urinae hominis's powder 12%, Rhizoma Trillii Tschonoskii dry powder 15%, kohlrabi leaf powder 26%, big Wheat bubble powder 7%, Semen Setariae italicae fine powder 20%, pine needle meal 14%, Folium Artemisiae Argyi extract powder 6% are mixed, and obtain Chinese medicine additive;
(5) mixing granulation:The above-mentioned oxygenation composition handled well, complex micro organism fungicide, adsorbent are mixed, growth is added Accelerator, Chinese medicine additive, synergist sodium perchlorate, stabilizer sodium metasilicate, sustained release agent sodium alginate, input pressure machine are pressed into Particle, grain graininess is controlled at 5 millimeters, is packaged as finished product, obtains removing the compound oxygenation agent of cultivation water nitrite.
Embodiment 2
A kind of compound oxygenation agent of removal cultivation water nitrite of the present embodiment, is prepared by the component of following weight portion Form:
45 parts of oxygenation composition, 31 parts of complex micro organism fungicide, 17 parts of adsorbent, 1.75 parts of growth promoter are (by weight ratio It is 1:1:2:2 Sodium Pyruvate, arsanilic acid, potassium chloride and calcium sulfate composition), 8.5 parts of Chinese medicine additive, sodium perchlorate 0.05 part, 2 parts of magnesium silicate, 1.8 parts of sodium alginate;
Its specific preparation method is as follows:
(1) oxygenation composition treatment:By weight ratio it is 1:4 weigh percarbamide and coated sodium peracrbonate, are placed on mixer It is interior, the adipic acid relative to percarbamide and coated sodium peracrbonate gross weight 16.5% is added, mixed 12.5 minutes with mixer, It is dry mixed-powder to be well mixed, and obtains oxygenation composition;
(2) complex micro organism fungicide is prepared:By denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, side spore gemma Bacillus, bacillus subtilis, red city Rhodococcus sp, silicate bacteria, streptococcus fecalis, the bacterial strain of bacillus coagulans are respectively adopted height Density fluid is fermented, then tunning is extracted and refined, and is made hyperconcetration bacterium powder, and the gemma of the hyperconcetration bacterium powder contains Amount reaches 1012CFU/g, by denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, bacillus laterosporus, bacillus subtilis Bacterium, red city Rhodococcus sp, silicate bacteria, streptococcus fecalis, the part by weight of bacillus coagulans are 3:1:2:2:3:1:1:0.5:1 Above-mentioned various bacterium powders are mixed, complex micro organism fungicide is obtained, it is stand-by;
(3) adsorbent is prepared:By weight ratio it is 4:2:1:1:0.5:0.3 weigh medical stone, atlapulgite, zeolite powder, White carbon, superfine silica gel powder, calcium phosphate, are crushed respectively, are crushed to grain fineness for 0.75mm, and mixing obtains mixed-powder, Through with 3.5% dilute sulfuric acid 1 is pressed with the mixed-powder:0.8 ratio acidification 45h at ambient temperature, in 320 DEG C of temperature 1.5h is calcined under the conditions of degree, is cooled down, adsorbent is obtained, it is stand-by;
(4) Chinese medicine additive is prepared:
A. calamitas urinae hominis's treatment:By calamitas urinae hominis with 30 DEG C of water process 7.5min, 45 DEG C for the treatment of 16min in baking oven are placed in, put Fried in frying pan to burned black, pulverization after cooling, obtain calamitas urinae hominis's powder, it is stand-by;
B. Rhizoma Trillii Tschonoskii treatment:By the dry rhizome portion of Rhizoma Trillii Tschonoskii with 30 DEG C of water process 0.75min, dry, use pulverizer Fine powder is ground into, 120 mesh sieve repeatedly, obtains Rhizoma Trillii Tschonoskii dry powder, it is stand-by;
C. kohlrabi leaf treatment:Kohlrabi leaf is cleaned up, after drying, sesame oil is added in frying pan, heated, treat sesame oil temperature When reaching 220 DEG C, kohlrabi leaf is put into sesame oil and fries 4min, kohlrabi leaf is drained, pulled out, ground after cooling, obtain kohlrabi leaf Powder, it is stand-by;
D. barley bubble treatment:Barley bubble root skin is taken, is cleaned up, dried, be ground into meal, be placed in boiler and add water to cook Extract 2 times, each 45min, filtering, merging filtrate is condensed into medicinal extract, then be spray-dried and obtain barley bubble extract powder, by filter residue Dry, pulverize, cross 110 mesh sieves, merge with barley bubble extract powder, that is, obtain barley bubble powder, it is stand-by;
E. Semen Setariae italicae treatment:Semen Setariae italicae is cleaned, 100 DEG C of baking 15min in baking oven are placed in, taken out, be placed in frying pan and fry To well-done, cooling, pulverization obtains Semen Setariae italicae fine powder, stand-by;
F. pine needle treatment:Pine needle edible tender branch and needle are taken, is cleaned up, dried naturally, crushed with pulverizer, 100 mesh Sieving, obtains pine needle meal, stand-by;
G. folium artemisiae argyi treatment:Folium artemisiae argyi is cleaned, is placed in boiler and is added water to cook extraction, the water for being added is folium artemisiae argyi weight 16.5 times, 105 DEG C of decoction 50min, filtering, filtrate concentrates and is spray-dried and obtains Folium Artemisiae Argyi extract powder, stand-by;
By above-mentioned calamitas urinae hominis's powder, Rhizoma Trillii Tschonoskii dry powder, kohlrabi leaf powder, barley bubble powder, Semen Setariae italicae fine powder, pine needle meal and Chinese mugwort Leaf extract powder presses following weight/mass percentage composition:It is calamitas urinae hominis's powder 12%, Rhizoma Trillii Tschonoskii dry powder 15%, kohlrabi leaf powder 26%, big Wheat bubble powder 7%, Semen Setariae italicae fine powder 20%, pine needle meal 14%, Folium Artemisiae Argyi extract powder 6% are mixed, and obtain Chinese medicine additive;
(5) mixing granulation:The above-mentioned oxygenation composition handled well, complex micro organism fungicide, adsorbent are mixed, growth is added Accelerator, Chinese medicine additive, synergist sodium perchlorate, stabilizer magnesium silicate, sustained release agent sodium alginate, input pressure machine are pressed into Particle, grain graininess is controlled at 12.5 millimeters, is packaged as finished product, obtains removing the compound oxygenation agent of cultivation water nitrite.
Embodiment 3
A kind of compound oxygenation agent of removal cultivation water nitrite of the present embodiment, is prepared by the component of following weight portion Form:
50 parts of oxygenation composition, 38 parts of complex micro organism fungicide, 29 parts of adsorbent, 2.5 parts of growth promoter (are by weight ratio 1:1:2:2 Sodium Pyruvate, arsanilic acid, potassium chloride and calcium sulfate composition), 10 parts of Chinese medicine additive, sodium perchlorate 0.12 part, 3 parts of nitrilo trimethylene phosphoric acid, 2.2 parts of polyvinyl alcohol;
Its specific preparation method is as follows:
(1) oxygenation composition treatment:By weight ratio it is 1:5 weigh percarbamide and coated sodium peracrbonate, are placed on mixer It is interior, the adipic acid relative to percarbamide and coated sodium peracrbonate gross weight 25% is added, mixed 20 minutes with mixer, mixing It is uniformly dry mixed-powder, obtains oxygenation composition;
(2) complex micro organism fungicide is prepared:By denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, side spore gemma Bacillus, bacillus subtilis, red city Rhodococcus sp, silicate bacteria, streptococcus fecalis, the bacterial strain of bacillus coagulans are respectively adopted height Density fluid is fermented, then tunning is extracted and refined, and is made hyperconcetration bacterium powder, and the gemma of the hyperconcetration bacterium powder contains Amount reaches 1013CFU/g, by denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, bacillus laterosporus, bacillus subtilis Bacterium, red city Rhodococcus sp, silicate bacteria, streptococcus fecalis, the part by weight of bacillus coagulans are 3:1:2:2:3:1:1:0.5:1 Above-mentioned various bacterium powders are mixed, complex micro organism fungicide is obtained, it is stand-by;
(3) adsorbent is prepared:By weight ratio it is 4:2:1:1:0.5:0.3 weigh medical stone, atlapulgite, zeolite powder, White carbon, superfine silica gel powder, calcium phosphate, are crushed respectively, are crushed to grain fineness for 1.2mm, and mixing obtains mixed-powder, Through with 6% dilute sulfuric acid 1 is pressed with the mixed-powder:0.8 ratio acidification 60h at ambient temperature, in 340 DEG C of temperature Under the conditions of calcine 2h, cool down, be obtained adsorbent, it is stand-by;
(4) Chinese medicine additive is prepared:
A. calamitas urinae hominis's treatment:By calamitas urinae hominis with 35 DEG C of water process 10min, 50 DEG C for the treatment of 20min in baking oven are placed in, be placed in Fried in frying pan to burned black, pulverization after cooling, obtain calamitas urinae hominis's powder, it is stand-by;
B. Rhizoma Trillii Tschonoskii treatment:By the dry rhizome portion of Rhizoma Trillii Tschonoskii with 35 DEG C of water process 1min, dry, crushed with pulverizer Into fine powder, 120 mesh sieve repeatedly, obtain Rhizoma Trillii Tschonoskii dry powder, stand-by;
C. kohlrabi leaf treatment:Kohlrabi leaf is cleaned up, after drying, sesame oil is added in frying pan, heated, treat sesame oil temperature When reaching 250 DEG C, kohlrabi leaf is put into sesame oil and fries 5min, kohlrabi leaf is drained, pulled out, ground after cooling, obtain kohlrabi leaf Powder, it is stand-by;
D. barley bubble treatment:Barley bubble root skin is taken, is cleaned up, dried, be ground into meal, be placed in boiler and add water to cook Extract 3 times, each 60min, filtering, merging filtrate is condensed into medicinal extract, then be spray-dried and obtain barley bubble extract powder, by filter residue Dry, pulverize, cross 120 mesh sieves, merge with barley bubble extract powder, that is, obtain barley bubble powder, it is stand-by;
E. Semen Setariae italicae treatment:Semen Setariae italicae is cleaned, 120 DEG C of baking 20min in baking oven are placed in, taken out, be placed in frying pan and fry To well-done, cooling, pulverization obtains Semen Setariae italicae fine powder, stand-by;
F. pine needle treatment:Pine needle edible tender branch and needle are taken, is cleaned up, dried naturally, crushed with pulverizer, 120 mesh Sieving, obtains pine needle meal, stand-by;
G. folium artemisiae argyi treatment:Folium artemisiae argyi is cleaned, is placed in boiler and is added water to cook extraction, the water for being added is folium artemisiae argyi weight 18 times, 110 DEG C of decoction 60min, filtering, filtrate concentrates and is spray-dried and obtains Folium Artemisiae Argyi extract powder, stand-by;
By above-mentioned calamitas urinae hominis's powder, Rhizoma Trillii Tschonoskii dry powder, kohlrabi leaf powder, barley bubble powder, Semen Setariae italicae fine powder, pine needle meal and Chinese mugwort Leaf extract powder presses following weight/mass percentage composition:It is calamitas urinae hominis's powder 12%, Rhizoma Trillii Tschonoskii dry powder 15%, kohlrabi leaf powder 26%, big Wheat bubble powder 7%, Semen Setariae italicae fine powder 20%, pine needle meal 14%, Folium Artemisiae Argyi extract powder 6% are mixed, and obtain Chinese medicine additive;
(5) mixing granulation:The above-mentioned oxygenation composition handled well, complex micro organism fungicide, adsorbent are mixed, growth is added Accelerator, Chinese medicine additive, synergist sodium perchlorate, stabilizer magnesium silicate, sustained release agent sodium alginate, input pressure machine are pressed into Particle, grain graininess is controlled at 20 millimeters, is packaged as finished product, obtains removing the compound oxygenation agent of cultivation water nitrite.
The measure of merit of the compound oxygenation agent of the gained removal cultivation water nitrite of embodiment 1~3 is as follows:
1. test material:There is part floating head phenomenon in certain cultivation water pond fish, takes cultivation water sample 60L, determines its oxygen content And nitrogen content, wherein, dissolved oxygen amount is 2.04mg/L, and ammonia-nitrogen content is 0.33mg/L, and content of nitrite is 0.18mg/L, is shown Right indices are not in safe range.
2. method of testing:Above-mentioned 60L water samples are divided into 3 parts, respectively every part of 20L, are separately added into the embodiment of the present invention 1 To the 3 compound oxygenation agents for preparing, water sample index is measured at times.
3. test result:Water sample 1, water sample 2, the dissolved oxygen amount of the day part of water sample 3, ammonia-nitrogen content, content of nitrite data Respectively as shown in Figure 1, Figure 2, Figure 3 shows.
Can be seen that the compound oxygenation agent oxygenation of present invention preparation is rapid, long-acting by the result of Fig. 1~3, can effectively remove Ammonia nitrogen and nitrite, and degradation speed is fast.
Above-described embodiment is the present invention preferably implementation method, but embodiments of the present invention are not by above-described embodiment Limitation, it is other it is any without departing from Spirit Essence of the invention and the change, modification, replacement made under principle, combine, simplification, Equivalent substitute mode is should be, is included within protection scope of the present invention.

Claims (9)

1. it is a kind of remove cultivation water nitrite compound oxygenation agent, it is characterised in that:The oxygenation agent is by following weight portion Component be prepared from:
40~50 parts of oxygenation composition, 24~38 parts of complex micro organism fungicide, 5~29 parts of adsorbent, growth promoter 1.0~2.5 Part, 7~10 parts of Chinese medicine additive, 0.02~0.12 part of synergist, 1~3 part of stabilizer, 1.4~2.2 parts of sustained release agent;
Described complex micro organism fungicide by denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, bacillus laterosporus, Bacillus subtilis, red city Rhodococcus sp, silicate bacteria, streptococcus fecalis and bacillus coagulans composition;
Described oxygenation composition includes percarbamide and coated sodium peracrbonate;Described adsorbent be by medical stone, atlapulgite, Zeolite powder, white carbon, superfine silica gel powder and calcium phosphate composition;Described growth promoter be by Sodium Pyruvate, arsanilic acid, Potassium chloride and calcium sulfate are constituted;Described Chinese medicine additive is by calamitas urinae hominis, Rhizoma Trillii Tschonoskii, kohlrabi leaf, barley bubble, Semen Setariae italicae, pine Pin and folium artemisiae argyi are made.
2. it is according to claim 1 it is a kind of remove cultivation water nitrite compound oxygenation agent, it is characterised in that:It is described Denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, bacillus laterosporus, bacillus subtilis in complex micro organism fungicide Bacterium, red city Rhodococcus sp, the weight ratio between silicate bacteria, streptococcus fecalis and bacillus coagulans are 3:1:2:2:3:1:1: 0.5:1。
3. it is according to claim 1 it is a kind of remove cultivation water nitrite compound oxygenation agent, it is characterised in that:It is described Percarbamide is 1 with the weight ratio of coated sodium peracrbonate:(3~5);It is the medical stone, atlapulgite, zeolite powder, white carbon, micro- Weight ratio between powder silica gel and calcium phosphate is 4:2:1:1:0.5:0.3;The Sodium Pyruvate, arsanilic acid, potassium chloride Weight ratio and calcium sulfate between is 1:1:2:2;The quality percentage of the active ingredient of each component contains in described Chinese medicine additive Measure and be:Calamitas urinae hominis 12%, Rhizoma Trillii Tschonoskii 15%, kohlrabi leaf 26%, barley bubble 7%, Semen Setariae italicae 20%, pine needle 14%, folium artemisiae argyi 6%.
4. it is according to claim 1 it is a kind of remove cultivation water nitrite compound oxygenation agent, it is characterised in that:It is described Synergist refer to sodium perchlorate;Described stabilizer refers to silicate or phosphate;Described sustained release agent refers to sodium alginate Or polyvinyl alcohol.
5. the preparation method of the compound oxygenation agent of a kind of removal cultivation water nitrite described in any one of Claims 1 to 4, It is characterized in that including following preparation process:
By denitrifying bacteria, trichoderma harzianum, bacillus amyloliquefaciens, bacillus laterosporus, bacillus subtilis, the red ball in red city Bacterium, silicate bacteria, streptococcus fecalis, the bacterial strain of bacillus coagulans are respectively adopted high density liquid fermentation, then to tunning Extracted and refined, be made hyperconcetration bacterium powder, various bacterium powders are mixed, obtained complex micro organism fungicide;By oxygenation composition, again Close microbial bacterial agent to mix with adsorbent, add growth promoter, Chinese medicine additive, synergist, stabilizer and sustained release agent, mixing Granulation after uniform, obtains removing the compound oxygenation agent of cultivation water nitrite.
6. the preparation method of a kind of compound oxygenation agent for removing cultivation water nitrite according to claim 5, it is special Levy and be:Described hyperconcetration bacterium powder refers to that spore content reaches 1012~1013The bacterium powder of CFU/g;Described granulation refers to use Forcing press is pressed into the particle that granularity is 5~20 millimeters.
7. the preparation method of a kind of compound oxygenation agent for removing cultivation water nitrite according to claim 5, it is special Levy and be:Described oxygenation composition is prepared by the following method:Percarbamide and coated sodium peracrbonate are weighed, mixer is placed on It is interior, the lubricant relative to percarbamide and coated sodium peracrbonate gross weight 8%~25% is added, mix 5~20 with mixer Minute, obtain oxygenation composition;Described adsorbent is prepared by the following method:Weigh medical stone, atlapulgite, zeolite powder, white Carbon black, superfine silica gel powder and calcium phosphate, are crushed respectively, are crushed to grain fineness for 0.3~1.2mm, are mixed to get mixed powder End, then mixed-powder is processed, calcine, cool down through inorganic acidifying, and adsorbent is obtained.
8. the preparation method of a kind of compound oxygenation agent for removing cultivation water nitrite according to claim 7, it is special Levy and be:Described lubricant refers to polyoxyethylene monostearate or adipic acid;Described inorganic acidifying treatment refers to use 1% ~6% dilute sulfuric acid presses 1 with the mixed-powder:0.8 mass ratio, 30~60h of acidification at ambient temperature;Described Calcining refers to be calcined under conditions of temperature is for 300~340 DEG C.
9. the preparation method of a kind of compound oxygenation agent for removing cultivation water nitrite according to claim 5, it is special Levy is that described Chinese medicine additive is prepared by the following method:
By calamitas urinae hominis with 25~35 DEG C of 5~10min of water process, 40~50 DEG C of 12~20min for the treatment of in baking oven are placed in, are placed in stir-fry Fried to burned black, pulverization after cooling in pot, obtain calamitas urinae hominis's powder;
By the dry rhizome portion of Rhizoma Trillii Tschonoskii with 25~35 DEG C of 0.5~1min of water process, dry, fine powder be ground into pulverizer, 120 mesh sieve, and obtain Rhizoma Trillii Tschonoskii dry powder;
Kohlrabi leaf is cleaned up, after drying, sesame oil is added in frying pan, heated, when sesame oil temperature reaches 200~250 DEG C, Kohlrabi leaf is put into sesame oil and fries 3~5min, kohlrabi leaf is drained, pulled out, ground after cooling, obtain kohlrabi leaf powder;
Barley bubble root skin is taken, is cleaned up, dried, be ground into meal, be placed in boiler and add water to cook extraction 1~3 time, every time 30 ~60min, filtering, merging filtrate is condensed into medicinal extract, then is spray-dried and obtains barley bubble extract powder, and filter residue and drying is crushed, 100~120 mesh sieves are crossed, is merged with barley bubble extract powder, that is, obtain barley bubble powder;
Semen Setariae italicae is cleaned, 80~120 DEG C of 10~20min of baking in baking oven are placed in, taken out, be placed in frying pan and fry to well-done, it is cold But, pulverization, obtains Semen Setariae italicae fine powder;
Pine needle edible tender branch and needle are taken, is cleaned up, dried naturally, crushed with pulverizer, the sieving of 80~120 mesh obtains pine Pin powder;
Folium artemisiae argyi is cleaned, is placed in boiler and is added water to cook extraction, the water for being added is 15~18 times of folium artemisiae argyi weight, 100~ 110 DEG C of 40~60min of decoction, filtering, filtrate concentrates and is spray-dried and obtains Folium Artemisiae Argyi extract powder;
Above-mentioned calamitas urinae hominis's powder, Rhizoma Trillii Tschonoskii dry powder, kohlrabi leaf powder, barley bubble powder, Semen Setariae italicae fine powder, pine needle meal and folium artemisiae argyi are carried Thing powder is taken by following weight/mass percentage composition:Calamitas urinae hominis's powder 12%, Rhizoma Trillii Tschonoskii dry powder 15%, kohlrabi leaf powder 26%, barley bubble Powder 7%, Semen Setariae italicae fine powder 20%, pine needle meal 14%, Folium Artemisiae Argyi extract powder 6% are mixed, and obtain Chinese medicine additive.
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