CN116119834A - Water quality treating agent and application thereof in aquaculture - Google Patents

Water quality treating agent and application thereof in aquaculture Download PDF

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Publication number
CN116119834A
CN116119834A CN202211661885.0A CN202211661885A CN116119834A CN 116119834 A CN116119834 A CN 116119834A CN 202211661885 A CN202211661885 A CN 202211661885A CN 116119834 A CN116119834 A CN 116119834A
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China
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parts
water quality
hundred million
microbial inoculum
treatment agent
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CN202211661885.0A
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Chinese (zh)
Inventor
苏同兴
苏华山
张桂琴
牛菽媛
王宇
孙一凡
王颖杰
牛克平
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Beijing Renbotang Institute Of Traditional Chinese Medicine
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Beijing Renbotang Institute Of Traditional Chinese Medicine
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/342Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2305/00Use of specific compounds during water treatment
    • C02F2305/06Nutrients for stimulating the growth of microorganisms

Abstract

The invention provides a water quality treating agent and application thereof in aquaculture, and belongs to the technical field of water quality treatment. The water quality treating agent provided by the invention comprises the following components in parts by weight: 40-50 parts of EM microbial inoculum, 30-40 parts of saccharomycetes, 10-30 parts of bacillus subtilis and 1-10 parts of trichoderma longibrachiatum. The water quality treatment agent provided by the invention comprises an EM (effective microorganism) microbial inoculum, saccharomycetes, bacillus subtilis and trichoderma longibrachiatum, and the microbial inoculum can be matched with each other to quickly and effectively repair the culture water body and restore the water quality.

Description

Water quality treating agent and application thereof in aquaculture
Technical Field
The invention relates to the technical field of water quality treatment, in particular to a water quality treating agent and application thereof in aquaculture.
Background
With the development of aquaculture, water quality problems are increasingly prominent. The problems of aquatic diseases and aquatic quality caused by water quality are increasing. The cause of deterioration of water quality is mainly: (1) improper disinfection results in contaminated water quality; (2) The water becomes thin due to untimely nutrition supply, the nutrition is lost, and the yield of the aquatic products is reduced; (3) The microbial flora is unbalanced, beneficial microorganisms in water are reduced, and harmful microorganisms are dominant; (4) The water body is overnutrition, so that water body plants and microorganisms are expanded, and the living space of the aquatic products is occupied; (5) The high-temperature storm causes the hypoxia of water, blue algae burst and fish choking death.
Currently, methods for improving or maintaining aquaculture water quality mainly include physical methods, chemical methods and biological methods. Wherein, the physical method comprises proper water injection and water change, thus keeping the water fresh; proper oxygenation is carried out, and the water body is kept rich in dissolved oxygen. The chemical method comprises the steps of regularly applying limestone, adjusting the pH value of the water body, and keeping good pH; the water disinfectant is applied regularly. The biological method is mainly to apply a microbial preparation or an enzyme preparation. Among the above methods, the physical method has limited regulation capability and poor effect, and the chemical method has strong regulation capability and quick response, but has serious secondary pollution and is used for endangering the water quality safety for a long time. The microbial preparation in the biological method has various improving effects, has long-acting effect and little harm, and can be used as an ideal water quality treatment mode.
Disclosure of Invention
The invention aims to provide a water quality treating agent which is used for purifying aquaculture water, improving the environment of aquaculture water and improving the quality and yield of aquatic products.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a water quality treating agent which comprises the following components in parts by weight: 40-50 parts of EM microbial inoculum, 30-40 parts of saccharomycetes, 10-30 parts of bacillus subtilis and 1-10 parts of trichoderma longibrachiatum.
Preferably, the effective viable count of the EM microbial inoculum is 800-1200 hundred million/g.
Preferably, the effective viable count of the saccharomycetes is 150-250 hundred million/g.
Preferably, the effective viable count of the bacillus subtilis is 1000-2000 hundred million/g.
Preferably, the effective viable count of the trichoderma longibrachiatum is 10-50 hundred million/g.
Preferably, the water quality treating agent also comprises a nutrient matrix with the mass of 1-5 times of EM bacteria.
Preferably, the nutrient medium comprises the following components in parts by weight: 30-50 parts of glucose, 10-20 parts of sucrose, 10-30 parts of yeast extract, 10-20 parts of peptone, 1-10 parts of lysine and 1-5 parts of aspartic acid.
The invention also provides an application of the water quality treating agent in aquaculture.
The water quality treatment agent provided by the invention comprises an EM (effective microorganism) microbial inoculum, saccharomycetes, bacillus subtilis and trichoderma longibrachiatum, and the microbial inoculum can be matched with each other to quickly and effectively repair the culture water body and restore the water quality. The water quality treating agent provided by the invention is also matched with a nutrition matrix for use, wherein the nutrition matrix is the basis for survival and function of the microbial inoculum in a water environment, and the nutrition matrix is added into the water quality treating agent, so that the survival and rapid propagation of the microbial inoculum are facilitated.
Detailed Description
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
In the following examples, the EM bacterial agents were purchased from ataegus chemical technology limited; the yeast was purchased from Shijia Hua Ren feed limited; the bacillus subtilis is purchased from Jinan Huiteng chemical industry Co., ltd; the trichoderma longibrachiatum was purchased from the company of the sciences of the wheatgrass biology, guangzhou.
Example 1
The fungus agents, 45 parts of EM fungus agent, 35 parts of saccharomycete, 20 parts of bacillus subtilis and 5 parts of trichoderma longibrachiatum are weighed according to the following weight parts. Wherein the effective viable count of the EM microbial inoculum is 1000 hundred million/g; the effective viable count of the saccharomycetes is 200 hundred million/g; the effective viable count of the bacillus subtilis is 2000 hundred million/g; the effective viable count of the trichoderma longibrachiatum is 10 hundred million/g.
Meanwhile, a nutrient substrate with the mass 3 times of that of the EM microbial inoculum is also added in the water quality treatment. Comprises the following components in parts by weight: 40 parts of glucose, 15 parts of sucrose, 20 parts of yeast extract, 15 parts of peptone, 5 parts of lysine and 3 parts of aspartic acid. The components are uniformly mixed and then used for water quality treatment.
Example 2
The microbial inoculum, 40 parts of EM microbial inoculum, 30 parts of saccharomycetes, 10 parts of bacillus subtilis and 1 part of trichoderma longibrachiatum are weighed according to the following weight parts. Wherein the effective viable count of the EM microbial inoculum is 1200 hundred million/g; the effective viable count of the saccharomycetes is 250 hundred million/g; the effective viable count of the bacillus subtilis is 1000 hundred million/g; the effective viable count of the trichoderma longibrachiatum is 50 hundred million/g.
Meanwhile, a nutrient substrate with the mass 5 times of that of the EM microbial inoculum is also added in the water quality treatment. Comprises the following components in parts by weight: 30 parts of glucose, 20 parts of sucrose, 10 parts of yeast extract, 20 parts of peptone, 1 part of lysine and 5 parts of aspartic acid. The components are uniformly mixed and then used for water quality treatment.
Example 3
The fungus agents, 50 parts of EM fungus agent, 40 parts of saccharomycete, 30 parts of bacillus subtilis and 10 parts of trichoderma longibrachiatum are weighed according to the following weight parts. Wherein the effective viable count of the EM microbial inoculum is 800 hundred million/g; the effective viable count of the saccharomycetes is 150 hundred million/g; the effective viable count of the bacillus subtilis is 1500 hundred million/g; the effective viable count of the trichoderma longibrachiatum is 30 hundred million/g.
Meanwhile, a nutrient substrate with the mass of 4 times of that of the EM microbial inoculum is also added in the water quality treatment. Comprises the following components in parts by weight: 50 parts of glucose, 10 parts of sucrose, 30 parts of yeast extract, 10 parts of peptone, 10 parts of lysine and 1 part of aspartic acid. The components are uniformly mixed and then used for water quality treatment.
Comparative example 1
The present comparative example is different from example 1 in that no trichoderma longibrachiatum was added. Specific:
the bacterial agents, 45 parts of EM bacterial agent, 35 parts of saccharomycete and 25 parts of bacillus subtilis are weighed according to the following weight parts. Wherein the effective viable count of the EM microbial inoculum is 1000 hundred million/g; the effective viable count of the saccharomycetes is 200 hundred million/g; the effective viable count of the bacillus subtilis is 2000 hundred million/g.
Meanwhile, a nutrient substrate with the mass 3 times of that of the EM microbial inoculum is also added in the water quality treatment. Comprises the following components in parts by weight: 40 parts of glucose, 15 parts of sucrose, 20 parts of yeast extract, 15 parts of peptone, 5 parts of lysine and 3 parts of aspartic acid. The components are uniformly mixed and then used for water quality treatment.
Experimental example 1
The experiment is carried out on a carp culture base in Shandong province, and before the experiment starts, the cultured fishes have serious death phenomenon due to water quality deterioration, and the average death rate of the carps in the week before the experiment is 86 carps/day. The water treatment agents prepared in examples 1 to 3 and comparative example 1 were applied to 4 fish ponds as subjects. The application starts from 12 days of 6 months, the application is carried out every 3 days, 2 kg/mu each time, the application is carried out every 24 days of 6 months instead of every week, 1.5 kg/mu each time, and the experiment is terminated after the last application of the medicine in 15 days of 7 months. And recording the death condition of the carp. The results are shown in Table 1.
TABLE 1 different treatments for carp death conditions
Figure BDA0004014357160000031
Figure BDA0004014357160000041
Note that: the data in the table are all the statistics of the day except the last row.
As can be seen from the statistics of Table 1, examples 1-3 and comparative example 1 both control and reduce the mortality of carps within a week, but the water treatment agent of examples 1-3 was significantly more efficient during the subsequent remediation process, and the mortality of carps of examples 1-3 was very low and tended to be essentially dead during the next two weeks, while the number of deaths per day of comparative example 1 remained above 10, and the comparative example still took at least 1-2 weeks to reach the current level of remediation of the examples from a continuous statistical record. Therefore, the water quality treating agent provided by the invention is matched with the trichoderma longibrachiatum, so that the treating efficiency is higher and the effect is better under the synergistic effect of all the microbial agents.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims (8)

1. The water quality treating agent is characterized by comprising the following components in parts by weight: 40-50 parts of EM microbial inoculum, 30-40 parts of saccharomycetes, 10-30 parts of bacillus subtilis and 1-10 parts of trichoderma longibrachiatum.
2. The water treatment agent according to claim 1, wherein the effective viable count of the EM bacterial agent is 800 to 1200 hundred million/g.
3. The water treatment agent according to claim 2, wherein the effective viable count of the yeast is 150 to 250 hundred million/g.
4. The water treatment agent according to claim 3, wherein the effective viable count of the bacillus subtilis is 1000 to 2000 hundred million/g.
5. The water treatment agent according to claim 4, wherein the number of viable bacteria of the trichoderma longibrachiatum is 10 to 50 hundred million/g.
6. The water treatment agent according to claim 5, further comprising a nutrient medium having a mass of 1 to 5 times that of the EM bacteria.
7. The water treatment agent according to claim 6, wherein the nutrient medium comprises the following components in parts by weight: 30-50 parts of glucose, 10-20 parts of sucrose, 10-30 parts of yeast extract, 10-20 parts of peptone, 1-10 parts of lysine and 1-5 parts of aspartic acid.
8. Use of a water treatment agent according to any one of claims 1 to 7 in aquaculture.
CN202211661885.0A 2022-12-23 2022-12-23 Water quality treating agent and application thereof in aquaculture Pending CN116119834A (en)

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CN114958641A (en) * 2021-09-27 2022-08-30 上海玖霖环保科技有限公司 Environment-friendly biological agent for treating livestock and poultry manure organic garbage
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