CN114262065A - Micro-ecological preparation for purifying aquaculture tail water - Google Patents
Micro-ecological preparation for purifying aquaculture tail water Download PDFInfo
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- CN114262065A CN114262065A CN202111407964.4A CN202111407964A CN114262065A CN 114262065 A CN114262065 A CN 114262065A CN 202111407964 A CN202111407964 A CN 202111407964A CN 114262065 A CN114262065 A CN 114262065A
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Abstract
The application discloses a microecological preparation for purifying aquaculture tail water, and relates to the technical field of biological preparations; the composition is prepared from the following raw materials in parts by weight: 50-80 parts of EM microbial inoculum and 50-100 parts of nutritional agent; the EM microbial inoculum consists of the following microbial inoculum in parts by weight: 40-50 parts of bacillus aceticus, 10-15 parts of bacillus agent and 10-20 parts of nitrobacteria agent; the application provides a microecologics safety ring protects, and can make aquaculture water organic matter reduce, and ammonia nitrogen, nitrite, hydrogen sulfide content and bottom of the pool silt chemical oxygen demand reduce by a wide margin, under the prerequisite of considering the cost of manufacture, effectively improve the water environment of aquaculture tail water, make it can realize reuse, promote the circulation of matter in the water.
Description
Technical Field
The application relates to the technical field of biological agents, in particular to a microecological agent for purifying aquaculture tail water and a preparation method thereof.
Background
In recent years, the development of aquaculture industry is rapid, the scale of intensive high-density breeding is continuously enlarged, and the development of social economy is promoted to a certain extent, however, the intensive high-density breeding applied for increasing the yield per unit area causes the destruction of the breeding ecological environment, and greatly increases the chance of pathogen cross infection among aquatic animals. Therefore, various fish diseases occur frequently, even aquatic animals die in a large area, and the culture water body environment is worsened. Aiming at the prevention and treatment of fish diseases, the traditional method mainly uses antibiotics and certain chemical synthetic drugs as feed additives or fishery drugs in large quantity. The long-term use of a large amount of antibiotics and chemical synthetic drugs can not only destroy and interfere the normal microflora of the culture environment to cause the ecological imbalance of microorganisms, but also cause the residual quantity of the antibiotics and the chemical synthetic drugs in aquatic products to be too much, and the antibiotics and the chemical synthetic drugs enter human bodies through food chains to be enriched to cause potential harm to human health, and the microbial ecological agent, namely a live microbial agent prepared by using normal microorganisms or substances for promoting the growth of the microorganisms, can overcome the defects of dysbacteriosis, double infection, drug-resistant strain enhancement and the like caused by the application of the antibiotics.
The microecological preparation achieves the purpose of preventing and treating diseases by purifying aquaculture water, improving animal immunity and improving aquaculture environment, reduces the harm of water eutrophication to aquatic animals and human beings, is an ideal substitute for antibiotics and other substances, is a new technical means, and has important significance for guaranteeing the safety of aquatic products and maintaining the health and sustainable development of aquaculture industry.
Therefore, the development of a method which can effectively improve the water quality condition of the aquaculture tail water, effectively solve the water body problems of high ammonia nitrogen and high COD in the water body, improve the water body quality and realize sustainable utilization is urgently needed.
Disclosure of Invention
The application aims to provide a microecologics for aquaculture tail water purifies, this microecologics safety ring protects, and can make aquaculture water organic matter reduce, and ammonia nitrogen, nitrite, hydrogen sulfide content and bottom of the pool silt chemical oxygen demand reduce by a wide margin, under the prerequisite of considering the cost of manufacture, effectively improve the water environment of aquaculture tail water, make it can realize reuse, promote the circulation of matter in the water.
In order to achieve the technical purpose, the application provides a microecological preparation for purifying aquaculture tail water, which is prepared from the following raw materials in parts by weight: 50-80 parts of EM microbial inoculum and 50-100 parts of nutritional agent;
the EM microbial inoculum consists of the following microbial inoculum in parts by weight: 40-50 parts of bacillus aceticus, 10-15 parts of bacillus subtilis and 10-20 parts of nitrobacteria.
Preferably, the acetobacter aceti agent comprises acetobacter cacao and acetobacter lofani;
preferably, the Acetobacter cacao has the Latin article name of Acetobacter fabarum and the preservation number of CICC No. 10883;
preferably, the Acetobacter lovaniensis has the Latin article name of Acetobacter lovaniensis and the deposit number of ATCC No. 12875;
preferably, the ratio of the acetobacter cacao to the acetobacter lofani is 1: 1;
preferably, the Bacillus agent is Bacillus subtilis, the Latin name of the Bacillus agent is Bacillus subtilis, and the preservation number of the Bacillus agent is CICC No. 10028;
preferably, the nutrient is prepared from the following raw materials in parts by weight: 5-15 parts of glucose, 1-5 parts of brown sugar and 1-5 parts of soybean meal;
preferably, the feed additive is prepared from the following raw materials in parts by weight: 60 parts of EM microbial inoculum, 80 parts of nutrient and 3 parts of thickening agent;
the EM microbial inoculum consists of the following microbial inoculum in parts by weight: 50 parts of bacillus aceticus, 12 parts of bacillus subtilis and 10 parts of nitrobacteria
Compared with the prior art, the beneficial effect of this application lies in:
(1) in the method, strain screening is carried out on the culture tail water, dominant strains in the water body are finally screened out through multiple enrichment culture, specific genera of the strains are obtained through sequencing analysis, the strains separated and screened out are tested independently and are subjected to compound testing, externally purchased microbial agents are also subjected to corresponding compound testing, corresponding strains on the market are purchased according to the specific genera of the screened strains for testing, and the optimal strain combination and strain proportion are finally obtained;
(2) the method has the advantages that the dominant strains are obtained from the culture wastewater, are reasonably compounded subsequently and are finally applied to the water body treatment, the strains are better in adaptability and effect, the ideal effect on the water body purification effect is easier to achieve, the new effect of some strains is easy to discover, the cacao acetobacter cacao and the Lofani acetobacter are blended and are not applied to the water body purification field before being screened and identified, the strains are screened and identified, similar strains which can be purchased from the market are tried to be compounded, and the remarkable effect is finally obtained;
(3) the EM microbial inoculum comprises nitrifying bacteria, and the nitrifying bacteria can convert ammonia nitrogen into nitrite through nitrification and further convert the nitrite into nitrate. The content of ammoniacal nitrogen and nitrite nitrogen in the aquaculture water body or the aquaculture wastewater can be reduced by utilizing nitrifying bacteria, so that the purposes of improving the water quality of the aquaculture water body and purifying the aquaculture wastewater are achieved;
(4) the EM microbial inoculum comprises bacillus subtilis which can absorb and utilize COD, ammonia nitrogen, nitrite and the like in the culture water in the growth process, and has better water purification effect; the bacillus subtilis can secrete a large amount of active substances such as subtilin, polymyxin, nystatin, short bacitracin, protease, amylase, lipase and the like in the growth process of the bacillus subtilis, can occupy the space advantage of flora, inhibit the growth and the propagation of harmful microorganisms such as harmful bacteria, pathogenic bacteria and the like, can decompose macromolecular organic matters such as residual feed, excrement and the like in a water body, and reduce the eutrophication degree of the water body; the bacillus subtilis can stimulate the growth and development of aquatic animals, improve the levels of immunoglobulin and antibody, enhance the cellular immunity and humoral immunity functions and improve the immunity of groups;
(5) the nutrient adopts the raw materials of glucose, brown sugar and bean pulp which are easy to obtain and low in cost, can effectively control the practical application cost in practical application, and simultaneously fully ensures that the EM microbial inoculum has sufficient carbon source and nitrogen source.
In conclusion, the microecological preparation provided by the application is green and environment-friendly, has no side effect on aquatic animals, is easy to obtain raw materials, convenient to use and low in cost, provides a new way for purifying aquaculture water, and has good social and economic benefits; second, the microecologics that this application provided improves the decomposition efficiency to organic pollutant to increase pond dissolved oxygen content, improve the water dissolved oxygen situation, improve aquaculture water body environment, make the breed tail water obtain fully purified, realize quick reuse.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without inventive exercise.
FIG. 1 is a table of experimental results for various examples regarding pH indicators;
FIG. 2 is a table of experimental results for DO indicators for various examples;
FIG. 3 is a table of experimental results for COD index for each example;
FIG. 4 is a table of experimental results of various examples regarding ammonia nitrogen indicators;
FIG. 5 is a table of experimental results for nitrite for various examples;
FIG. 6 is a table of experimental results for hydrogen sulfide for various examples;
Detailed Description
In order to make the objects, features and advantages of the present invention comprehensible, specific embodiments accompanied with examples are described in detail below. Several embodiments of the invention are given below. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete:
example 1
(1) Preparing Acetobacter xylinum Acetobacter xylinum (Acetobacter fabarum, preservation number is CICC No.10883) from the inclined plane of the test tube, inoculatingIn 30-100 mL of sterilized acetic acid bacteria culture medium, at 30-32 deg.C and 100--1Culturing on shaker for 22-26h, inoculating into 0.6-0.8L sterilized acetic acid bacteria culture medium, culturing at 30-32 deg.C and 100--1Culturing on shaking table for 22-26h to obtain strain seed solution with viable count of 108 CFU.mL or more-1。
Acetobacter lofani (Acetobacter lovaniensis, deposit number ATCC No.12875) cultures corresponding strain seed liquid according to the culture method;
mixing two acetobacter with a ratio of 1:1 to prepare an acetobacter agent;
the acetic acid bacteria culture medium comprises 98 g.L of glucose-19 g.L yeast extract-1Calcium carbonate 20 g.L-1Adjusting pH to about 7.2, sterilizing at 121 deg.C for 15min, and cooling
(2) Preparation of bacillus agent
Taking a ring of Bacillus subtilis (CICC No.10028) lawn from the inclined plane of the test tube, inoculating the lawn into 50mL sterilized broth culture medium, and culturing at 32 ℃ for 120r min-1Culturing on a shaking table for 24 h. Then inoculating into 1L sterilized broth culture medium, and culturing at 32 deg.C for 120r min-1Culturing on shaking table for 36h to obtain strain seed solution. The viable count of the bacteria liquid is more than or equal to 109 CFU.mL-1。
Wherein the broth culture medium contains peptone 12 g.L-16 g.L beef extract-1，NaCl5g·L-1Adjusting pH to about 7.2, sterilizing at 121 deg.C for 20min, and cooling.
Wherein the nitrifying bacteria are purchased from Biwofeng biotechnology (Guangdong) GmbH, are in powder form, can be directly added into water body for activation, and have viable count of 1 × 1010 cfu/g.
The embodiment provides a microecological preparation for purifying aquaculture tail water, which is prepared from the following raw materials in parts by weight: 50 parts of EM microbial inoculum and 50 parts of nutritional agent;
wherein the EM microbial inoculum consists of the following microbial inoculum in parts by weight: 45 parts of bacillus aceticus, 10 parts of bacillus agent and 10 parts of nitrifying bacteria agent;
the nutrient is prepared from the following raw materials in parts by weight: 5 parts of glucose, 1 part of brown sugar and 5 parts of soybean meal.
The preparation method comprises the steps of mixing seed solutions of various microbial agents in the proportion to prepare the EM microbial agent, preparing the nutrient in the proportion of the raw materials of the nutrient, and finally mixing the EM microbial agent and the nutrient to prepare the microecological preparation.
Example 2
The seed liquid configuration of each strain in the EM microbial inoculum in the embodiment refers to embodiment 1, and the difference is that the mixture ratio of each raw material is different, and the preparation method is the same;
the embodiment provides a microecological preparation for purifying aquaculture tail water, which is prepared from the following raw materials in parts by weight: 60 parts of EM microbial inoculum and 80 parts of nutritional agent;
wherein the EM microbial inoculum consists of the following microbial inoculum in parts by weight: 40 parts of bacillus aceticus, 15 parts of bacillus agent and 10 parts of nitrobacteria agent;
the nutrient is prepared from the following raw materials in parts by weight: 10 parts of glucose, 3 parts of brown sugar and 3 parts of soybean meal.
The preparation method comprises the steps of mixing seed solutions of various microbial agents in the proportion to prepare the EM microbial agent, preparing the nutrient in the proportion of the raw materials of the nutrient, and finally mixing the EM microbial agent and the nutrient to prepare the microecological preparation.
Example 3
The seed liquid configuration of each strain in the EM microbial inoculum in the embodiment refers to embodiment 1, and the difference is that the mixture ratio of each raw material is different, and the preparation method is the same;
the embodiment provides a microecological preparation for purifying aquaculture tail water, which is prepared from the following raw materials in parts by weight: 80 parts of EM microbial inoculum and 100 parts of nutritional agent;
wherein the EM microbial inoculum consists of the following microbial inoculum in parts by weight: 40 parts of acetic acid bacillus agent, 15 parts of bacillus agent and 20 parts of nitrifying bacteria agent;
the nutrient is prepared from the following raw materials in parts by weight: 15 parts of glucose, 5 parts of brown sugar and 5 parts of soybean meal.
The preparation method comprises the steps of mixing seed solutions of various microbial agents in the proportion to prepare the EM microbial agent, preparing the nutrient in the proportion of the raw materials of the nutrient, and finally mixing the EM microbial agent and the nutrient to prepare the microecological preparation.
The microbial formulations prepared in examples 1-3 were applied to three culture ponds of the same size, having an area of about 3 acres and a depth of 1.5 meters, available from the market and filled with similar water purifiers, and the results are shown in fig. 1-6.
As can be seen from the tables in fig. 1-6, when the microecologics provided by the present application are used for treating the culture tail water, the treatment indexes are significantly different from those of the water purifiers purchased on the market;
comparing the three embodiments, wherein the embodiment 2 has the best effect, the pH value, ammonia nitrogen, nitrite, hydrogen sulfide and sediment COD in the aquaculture water body are all obviously reduced, and the dissolved oxygen is greatly increased; so can realize breeding the purification of tail water and recycling, realize good economic benefits when guaranteeing green.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict.
The above description is only for the preferred embodiment of the present application and should not be taken as limiting the present application in any way, and all simple modifications, equivalent changes and modifications made to the above embodiment according to the technical spirit of the present application are intended to be included within the scope of the present application.
Claims (8)
1. A microecological preparation for purifying aquaculture tail water is characterized in that: the composition is prepared from the following raw materials in parts by weight: 50-80 parts of EM microbial inoculum and 50-100 parts of nutritional agent;
the EM microbial inoculum consists of the following microbial inoculum in parts by weight: 40-50 parts of bacillus aceticus, 10-15 parts of bacillus subtilis and 10-20 parts of nitrobacteria.
2. The microecological formulation according to claim 1, wherein: the acetobacter aceti agent comprises acetobacter cacao and acetobacter lofani.
3. The microecological formulation according to claim 1, wherein: the Latin article name of the Acetobacter cacao is Acetobacter fabarum, and the preservation number is CICC No. 10883.
4. The microecological formulation according to claim 1, wherein: the Latin article of Acetobacter lovaniensis is named Acetobacter lovaniensis and has a deposit number of ATCC No. 12875.
5. The microecological formulation according to claim 2, wherein: the ratio of the acetobacter cacao to the acetobacter lofani is 1: 1.
6. The microecological formulation according to claim 1, wherein: the Bacillus agent is Bacillus subtilis, the Latin name of the Bacillus agent is Bacillus subtilis, and the preservation number of the Bacillus agent is CICC No. 10028.
7. The microecological formulation according to claim 1, wherein: the nutritional agent is prepared from the following raw materials in parts by weight: 5-15 parts of glucose, 1-5 parts of brown sugar and 1-5 parts of soybean meal.
8. The microecological formulation according to any one of claims 1 to 7, wherein: the composition is prepared from the following raw materials in parts by weight: 60 parts of EM microbial inoculum and 80 parts of nutritional agent;
the EM microbial inoculum consists of the following microbial inoculum in parts by weight: 50 parts of acetic acid bacillus agent, 12 parts of bacillus agent and 10 parts of nitrifying bacteria agent.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116119834A (en) * | 2022-12-23 | 2023-05-16 | 北京仁博堂中医研究院 | Water quality treating agent and application thereof in aquaculture |
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Patent Citations (5)
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| CN101147529A (en) * | 2007-10-31 | 2008-03-26 | 无锡中顺生物技术有限公司 | Microecological preparation for aquaculture and its preparation method |
| CN101962229A (en) * | 2010-06-23 | 2011-02-02 | 天津生机集团股份有限公司 | Water purifying agent for pond culture |
| CN104673695A (en) * | 2013-11-29 | 2015-06-03 | 大连三科生物工程有限公司 | Preparation method of aquiculture water body purification microbial inoculant |
| US20170002310A1 (en) * | 2014-06-03 | 2017-01-05 | Jianganan University | Efficient Bottom-Improving Bacillus and Compound Bottom-Improving Microbial Agent Prepared From the Same and Applications Thereof |
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| CN116119834A (en) * | 2022-12-23 | 2023-05-16 | 北京仁博堂中医研究院 | Water quality treating agent and application thereof in aquaculture |
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