CN102249389A - Application of PHA in aquiculture - Google Patents
Application of PHA in aquiculture Download PDFInfo
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- CN102249389A CN102249389A CN2011101102564A CN201110110256A CN102249389A CN 102249389 A CN102249389 A CN 102249389A CN 2011101102564 A CN2011101102564 A CN 2011101102564A CN 201110110256 A CN201110110256 A CN 201110110256A CN 102249389 A CN102249389 A CN 102249389A
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- pha
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses an application of PHA in aquiculture. A PHA-containing agent is adopted as a matrix for the solid-phase denitrification of water body and a biomembrane of large surface area is formed, thus providing energy and places for the dephosphorization and denitrification of microbes; in the meanwhile, the PHA-containing agent provides nutritional substances for beneficial microorganisms to make them propagate in large numbers, so as to restore the ecological environment of the water body, improve the water quality, increase dissolved oxygen, minimize the feeding amount of forage, establish an excellent eco-balance system and accomplish environmentally-friendly and sustainable high-density breeding.
Description
Technical field
The present invention relates to aquaculture, the particularly application of PHA in aquaculture.
Background technology
Tradition aquatic products aquaculture model, especially Ji Yuehua high-density breeding pattern are the mode of production that cost is kept mostly to consume a large amount of high protein feeds to pollute pond self and offshore environment; The raiser uses sterilizing agent, antibiotic etc in a large number in order to prevent and treat the fishes and shrimps disease in addition, havoc ecotope.A large amount of water sources are not only polluted in this vicious cycle, increase aquaculture cost, have also seriously restricted the development of fishes and shrimps healthy aquacultures.Studies show that the protein in the feed only has 20% to be utilized, about 80% nitrogen is discharged in the water surrounding, changes into deleterious ammonia-state nitrogen, nitrite nitrogen etc.; In sealing or semi-enclosed high-density cultivation system, the accumulation of inorganic nitrogen such as ammonia-state nitrogen, nitrite nitrogen is especially serious, brought very big harm for the healthy growth of fishes and shrimps, improve both at home and abroad that in the high-density cultivation system there be the method for water quality: sorbent materials such as the zeolite powder of 1, splashing in water body; 2, absorb inorganic nitrogen by cultivating plant plankton or macro; 3, use probiotics such as genus bacillus to transform inorganic nitrogens such as ammonia-state nitrogen, nitrite nitrogen; 4, in feed, reduce protein content, reduce the total nitrogen that adds in the cultivating system.Also there has been announcement much to utilize these methods to improve the patent of water quality, as: patent CN101269886A has invented a kind of prawn high-density cultivation system water quality cleansing agent, is formulated by organic carbon hydrate and probiotics (subtilis and Rhodopseudomonas palustris).Patent CN101200337A adopts polyolefins and polymeric amide compounded mix layer to remove organism, suspended substance, nitrogen and the phosphorus of culturing in the water.Patent CN101703028A repairs sediment of pond and aquaculture water by measures such as supplement trace nutrition in the ecosystem of pond, growth-promoting agent, toxinicide or input beneficial microorganisms.But at present existing these methods all can not be low-cost, continuous and effective ground plays the effect that purifies and nutritive substance is provided for the beneficial microorganism in the aquaculture simultaneously to the waters in the aquaculture.
Summary of the invention
For overcoming above-mentioned defective, the invention discloses the application of PHA (PHA) in aquaculture, can adopt PHA efficiently to remove deleterious ammonia-state nitrogen in the water body, objectionable impuritiess such as nitrous acid nitrogen, for beneficial microorganism provides nutritive substance, make its a large amount of breedings simultaneously, repair water ecological setting, improve water quality, the animals and plants that swim are bred in a large number, increase dissolved oxygen, reduce the feedstuff feeding amount, and set up a good eubiosis system, realize eco-friendly, continuable high-density breeding.
The invention discloses the application of PHA in aquaculture, it is characterized in that: in the waters, throw in the agent material that contains PHA.
Described dose of material injected volume is that 5-100g is thrown in one cube of square meter waters.
It is to throw in once in every 1-4 week that described dose of material thrown in frequency.
Described dose of material is pellet, powder or porous agent material.
Described PHA is one or more mixtures among PHB, P3HB4HB, PHBV, PHBHHx, PHO or the P3HB4HB3HP.
Described PHB, P3HB4HB, PHBV, PHBHHx, PHO or P3HB4HB3HP are meant the multipolymer of poly butyric ester, poly 3-hydroxy butyrate 4 hydroxybutyric acid ester, poly butyric ester valerate, poly butyric ester capronate, poly--3-Hydroxyoctanoic acid ester, poly-3-butyric ester-4 butyric ester 3-hydroxyl heptanoate respectively.
Described dose of material also comprises the inorganic mineral weighting agent.
Described inorganic mineral weighting agent is one or more in lime carbonate, talcum powder, white mica, kaolin, boron nitride, ground barium sulfate, glass microballon and the titanium dioxide.
The weight content of described inorganic mineral weighting agent is 1-60%.
Also contain other additives in the described dose of material, described other additives are one or more mixtures in oxidation inhibitor, micronutrient element, the growth stimulant.
Described oxidation inhibitor can be selected conventional oxidation inhibitor, micronutrient element and growth stimulant.
The weight content of described other additives in the agent material is 0.5%-10%.
Contain PHA in the of the present invention dose of material, PHA itself is exactly the carbon source and the energy in the bacterium, can directly be decomposed by beneficial microorganism (as genus bacillus, pseudomonas etc.) to absorb, and the growth that can be continuously probiotics provides organic carbon source, impels its a large amount of breedings.PHA can also form the very big microbial film of surface-area as the matrix of solid phase denitrogenation, and denitrogenating for the dephosphorization of microorganism provides the energy and place.PHA impels the breeding of probiotics, has purified water quality, and the growth for plant plankton simultaneously provides nutritive substance; The feasible animals and plants that swim increase fast, increase the water body dissolved oxygen, lower the feedstuff feeding amount simultaneously and improve cultivation density.PHA forms stable, good ecosystem in water body after, promote the beneficial microorganism breeding, suppressed the harmful microbe growth, can reduce the use of antibiotic etc, improve the quality of various aquatic products.
Also be added with the inorganic mineral additive in the of the present invention dose of material, suspended matter and metal ion on the one hand can the adsorption filtration water body, the mineral element in the inorganic mineral additive is of value to beneficial microorganism breeding in the water body on the other hand.The inorganic mineral content of additive is selected according to actual needs, and degradation speed that can the conditioning agent material is in order to avoid microorganism excessive multiplication or owing to nutritive ingredient causes breeding slow inadequately.
Embodiment
The present invention is described in detail below in conjunction with specific embodiment.
Embodiment 1:
Agent material in the present embodiment is that 80% P3H4HB (4HB content is 15%) and 20% lime carbonate are formed by weight content.
Embodiment 2:
Agent material in the present embodiment is that 90% PHBV (the HV amount is 10%) and 10% talcum powder are formed by weight content.
Embodiment 3:
Agent material in the present embodiment is that 70% PHBHHx (the HH amount is 15%) and 30% kaolin are formed by weight content.
Embodiment 4:
Test is carried out in the glass jar of 300L, cultures the pond from same cabrilla and fetches water, and adds 250L in each cylinder, puts into the long cabrilla of 50 tail 10cm at random.Do not change water in the process of the test, the cabrilla basal feed of throwing something and feeding at regular time and quantity every day; Blank and experimental group are under equal conditions carried out, and just experimental group is placed 2gPHB in the fish jar bottom when beginning, and water sample is extracted in 2 all backs, test inorganic nitrogen total amount, and the result is as follows:
| Group | Inorganic nitrogen (mg/L) |
| Blank | 25.3 |
| Experimental group (adding PHA) | 4.5 |
Embodiment 5:
Test in Shangrao City eel culture field, select 2 onesize Tu Chi for use, cultivation density is suitable, and No. 1 the pond is contrast; No. 2 ponds are test group, add the agent material among the embodiment 1 one time every 2 weeks, and injected volume is 10g/m
3Feed injected volume and feeding patterns are consistent, and carry out 2 months nursing.Content of inorganic nitrogen in and beneficial microorganism in water body of per two weeks test, the vegeto-animal quantity of swimming.The result shows, uses in No. 2 ponds of PHA, and the content of inorganic nitrogen has on average reduced by 73% in the water body, and microbial count has on average increased by 210%, and amount of algae has on average increased by 175%, and zooplankton quantity has on average increased by 118%; And every day oxygenating machine on time can shorten 1 hour.When off-test, all eels are salvaged, the discovery of weighing, the eel weight in No. 1 pond of eel weight ratio in No. 2 ponds exceeds 9%.This explanation additive after, water quality be improved significantly, beneficial microorganism is bred in a large number, the vegeto-animal growth that helps simultaneously swimming has improved the output in unit waters, significantly increases economic benefit.
Embodiment 6:
Plant tests Foochow filefish, selects 2 onesize breed ponds for use, and cultivation density is suitable, and No. 1 the pond is contrast; No. 2 ponds are test group, add the agent material among the embodiment 2 one time every 2 weeks, and injected volume is 100g/m
3Carry out 3 months experiment.Content of inorganic nitrogen in and beneficial microorganism in water body of per two weeks test; During off-test, all fishes are salvaged, the weighing gross weight, and calculate feed conversion rate.The result shows, uses in No. 2 ponds of PHA, and the content of inorganic nitrogen has on average reduced by 67% in the water body, and microbial count has on average increased by 189%.When off-test, all filefish are salvaged, the discovery of weighing, No. 2 ponds exceed 17% than the output in No. 1 pond, and feed conversion rate exceeds 21%.After this explanation additive, can save feed cost, improve output simultaneously for the raiser.
Embodiment 7:
Test permanently happy prawn culturing field in Foochow, selects 4 onesize breed ponds for use, and No. 1 the pond is contrast; 2,3, No. 4 ponds are test group, add the agent material among the embodiment 3 one time every 1 week, and injected volume is respectively 5g/m
3, 10g/m
3, 15g/m
3, carry out 1 month experiment.Be the maximum prawn culturing density under the existence conditions No. 1,2,3, No. 4 pond cultivation density when first week is suitable with No. 1 pond, since second week, add prawn respectively into 5%, 10%, 15%, culture after one month, find that the shrimp in four ponds grows fine, do not have ill phenomenon.Weigh after fishing for, find that prawn stature in four ponds, weight difference are few.This explanation under equal conditions after the additive, can improve the cultivation density of prawn, saves client's venue cost.
In sum, but the present invention is not limited to above-mentioned embodiment, and persons skilled in the art are in the disclosed technical scope of the present invention, and the variation that can expect easily is all within protection scope of the present invention.
Claims (10)
1.PHA the application in aquaculture is characterized in that: in the waters, throw in the agent material that contains PHA.
2. the application of PHA as claimed in claim 1 in aquaculture is characterized in that: described dose of material injected volume is that 5-100g is thrown in one cubic metre of waters.
3. the application of PHA as claimed in claim 1 in aquaculture is characterized in that: it is to throw in once in every 1-4 week that described dose of material thrown in frequency.
4. the application of PHA as claimed in claim 1 in aquaculture is characterized in that: described dose of material is pellet or powder or porous agent material.
5. the application of PHA as claimed in claim 1 in aquaculture is characterized in that: described PHA is one or more mixtures among PHB, P3HB4HB, PHBV, PHBHHx, PHO or the P3HB4HB3HP.
6. as any application of described PHA in aquaculture of claim 1-5, it is characterized in that: described dose of material also comprises the inorganic mineral weighting agent.
7. the application of PHA as claimed in claim 6 in aquaculture is characterized in that: described inorganic mineral weighting agent is one or more mixtures in lime carbonate, talcum powder, white mica, kaolin, boron nitride, ground barium sulfate, glass microballon and the titanium dioxide.
8. the application of PHA as claimed in claim 7 in aquaculture is characterized in that: the weight content of described inorganic filler is 1-60%.
9. the application of PHA as claimed in claim 6 in aquaculture is characterized in that: also contain other additives in the described dose of material, described other additives are one or more mixtures in oxidation inhibitor, micronutrient element, the growth stimulant.
10. the application of PHA as claimed in claim 9 in aquaculture is characterized in that: the weight content of described other additives in the agent material is 0.5%-10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101102564A CN102249389A (en) | 2011-04-29 | 2011-04-29 | Application of PHA in aquiculture |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101102564A CN102249389A (en) | 2011-04-29 | 2011-04-29 | Application of PHA in aquiculture |
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| CN102249389A true CN102249389A (en) | 2011-11-23 |
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| CN2011101102564A Pending CN102249389A (en) | 2011-04-29 | 2011-04-29 | Application of PHA in aquiculture |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004681A (en) * | 2012-12-31 | 2013-04-03 | 蒋石容 | Aquarium water activation device |
| CN105230548A (en) * | 2015-11-25 | 2016-01-13 | 上海海洋大学 | Tilapia high yield breeding method |
| CN111317074A (en) * | 2020-03-25 | 2020-06-23 | 湖州师范学院 | Water quality regulator for promoting formation of biological flocs and preparation method and use method thereof |
| CN115974277A (en) * | 2022-12-29 | 2023-04-18 | 中国水产科学研究院珠江水产研究所 | Method for reducing total nitrogen of aquaculture water by utilizing slow-release carbon source |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10101465A (en) * | 1996-09-25 | 1998-04-21 | Seto Seido Kk | Controlled release fertilizer |
| CN101703028A (en) * | 2009-11-16 | 2010-05-12 | 刘瀚昌 | Application method of bioremediation technology in aquiculture |
| CN101928069A (en) * | 2009-06-19 | 2010-12-29 | 深圳市意可曼生物科技有限公司 | Sewage purifier and sewage purification method |
| CN101928179A (en) * | 2009-06-19 | 2010-12-29 | 深圳市意可曼生物科技有限公司 | Slow release fertilizer having soil remediation function and soil contamination remediation method |
-
2011
- 2011-04-29 CN CN2011101102564A patent/CN102249389A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10101465A (en) * | 1996-09-25 | 1998-04-21 | Seto Seido Kk | Controlled release fertilizer |
| CN101928069A (en) * | 2009-06-19 | 2010-12-29 | 深圳市意可曼生物科技有限公司 | Sewage purifier and sewage purification method |
| CN101928179A (en) * | 2009-06-19 | 2010-12-29 | 深圳市意可曼生物科技有限公司 | Slow release fertilizer having soil remediation function and soil contamination remediation method |
| CN101703028A (en) * | 2009-11-16 | 2010-05-12 | 刘瀚昌 | Application method of bioremediation technology in aquiculture |
Non-Patent Citations (5)
| Title |
|---|
| 《Aquacultural Engineering》 20001231 A.Boley et al "Biodegradable polymers as solid substrate and biofilm carrier for denitrification in recirculated aquaculture systems" 1-10 第22卷, * |
| A.BOLEY ET AL: ""Biodegradable polymers as solid substrate and biofilm carrier for denitrification in recirculated aquaculture systems"", 《AQUACULTURAL ENGINEERING》 * |
| 宋盛宪等: ""常用的底质水质改良药物"", 《常见鱼虾病害的防治与饲料营养》 * |
| 李懋等: ""可生物降解材料聚羟基脂肪酸酯(PHA)的合成与应用概述"", 《环境科学与管理》 * |
| 段碧华: ""水产养殖中饲料添加剂的影响"", 《新农村环境保护与治理》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004681A (en) * | 2012-12-31 | 2013-04-03 | 蒋石容 | Aquarium water activation device |
| CN105230548A (en) * | 2015-11-25 | 2016-01-13 | 上海海洋大学 | Tilapia high yield breeding method |
| CN105230548B (en) * | 2015-11-25 | 2018-11-09 | 上海海洋大学 | A kind of Tilapia mossambica high-yield cultivation method |
| CN111317074A (en) * | 2020-03-25 | 2020-06-23 | 湖州师范学院 | Water quality regulator for promoting formation of biological flocs and preparation method and use method thereof |
| CN111317074B (en) * | 2020-03-25 | 2022-04-01 | 湖州师范学院 | Water quality regulator for promoting formation of biological flocs and preparation method and use method thereof |
| CN115974277A (en) * | 2022-12-29 | 2023-04-18 | 中国水产科学研究院珠江水产研究所 | Method for reducing total nitrogen of aquaculture water by utilizing slow-release carbon source |
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Application publication date: 20111123 |