CN1760146A - Nourishing water transfer agent for aquiculture - Google Patents

Nourishing water transfer agent for aquiculture Download PDF

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CN1760146A
CN1760146A CN 200510041478 CN200510041478A CN1760146A CN 1760146 A CN1760146 A CN 1760146A CN 200510041478 CN200510041478 CN 200510041478 CN 200510041478 A CN200510041478 A CN 200510041478A CN 1760146 A CN1760146 A CN 1760146A
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milliliter
bacterium
cell count
individual cell
water
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CN100494093C (en
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沈颂东
项文钰
王建伟
林阿朋
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Suzhou University
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Suzhou University
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Abstract

A nutritive water regulation for the culture of aquatic products is composed of a bacterial phase containing photosynthetic bacteria, yeast, Bacillus subtitis and lactobacillus, and an algal phase (chlorella). Its advantages are high effect to clean water and lower by-effect.

Description

Nourishing water transfer agent for aquiculture
Technical field
The present invention relates to the purification of water quality treatment technology of aquaculture systems such as fish pond, shrimp crab pond and pond, be specifically related to a kind of nutrition water adjusting agent of aquaculture system.
Background technology
The growing standard of living that improves people of aquaculture.Yet at present ubiquity the water pollution issue of aquaculture water in culture fishery.These pollute the feed ingredient that major part comes from input, owing to contain some nitric acid that can not be utilized, nitrous acid and ammonia salt component in the feeds such as fish, shrimp, crab, cause the some diseases outburst of fish, shrimp, crab after the accumulation easily, and output can't be increased, even large batch of death appears, therefore is badly in need of some and handles targetedly.
In recent years, also more and more higher to the water quality requirement of aquaculture water owing to a large amount of exploitations to fishery products, the research that therefore relevant water body purification of aquaculture is handled this respect also increases gradually.Such as, Chinese patent once disclosed name and had been called " a kind of compound microorganism ferments microbial inoculum, preparation method and its usage ", publication number: CN1511940A, and name is called " preparation of rich water composite bacteria and the method for repairing aquaculture environment ", publication number: applications for a patent for invention such as CN1580002A.The water adjusting agent that these patents are all formed with various microbial strainss comes the water quality environment of repairing aquaculture, and described microbial strains includes bacterial classifications such as photosynthetic bacterium, yeast, milk-acid bacteria and genus bacillus.Because the effect of every kind of bacterium is different, so the combination treatment effect of different sorts, different ratios bacterial classification is different, and specific aim also is different.But because the PH of above-mentioned four kinds of bacterium can reach 2.5~5.0, make water quality be weak acid even strongly-acid, the aquaculture water short period of time this state occurs and can play the effect of killing harmful bacterium of curing the disease, being in this state for a long time can cause flesh of fish qualitative change pine, the mouthfeel variation, existence to fry is also dangerous, influences output of aquatic products and quality.
Summary of the invention
The invention provides a kind of bacterial classification and algae and divide combined nourishing water transfer agent for aquiculture, its objective is and to solve in the prior art, use bacterial classification aquaculture system to be carried out the defective that exists in the purifying treatment separately, thereby further improve the net effect of aquaculture water purifying treatment, reduce the side effect of using bacterial classification purifying treatment aquaculture water separately.
For achieving the above object, the technical solution used in the present invention is: a kind of nourishing water transfer agent for aquiculture, by bacterium phase preparation and algae mutually preparation two portions phase-splitting assembly constitute, wherein:
Bacterium phase preparation is made up of following bacterial classification and content thereof:
Photosynthetic bacterium 5 * 10 8~5 * 10 10Individual cell count/milliliter;
Yeast 1 * 10 7~5 * 10 8Individual cell count/milliliter;
Subtilis 1 * 10 7~1 * 10 9Individual cell count/milliliter;
Milk-acid bacteria 1 * 10 7~5 * 10 8Individual cell count/milliliter;
Algae phase preparation is made up of following algae and content thereof:
Chlorella 1 * 10 8~1 * 10 10Individual cell count/milliliter.
Related content in the technique scheme is explained as follows:
1, in the such scheme, described " phase-splitting assembly " be meant nutrition water adjusting agent of the present invention by bacterium phase preparation and algae mutually preparation two portions form, these two portions are separately packed when product is sold, and should not be the biphase mixture.
2, in the such scheme, in order to improve effect, bacterium phase preparation is made up of following bacterial classification and content thereof preferably:
Photosynthetic bacterium 8 * 10 8~3 * 10 10Individual cell count/milliliter;
Yeast 3 * 10 7~3 * 10 8Individual cell count/milliliter;
Subtilis 5 * 10 7~5 * 10 8Individual cell count/milliliter;
Milk-acid bacteria 3 * 10 7~3 * 10 8Individual cell count/milliliter;
Algae phase preparation is made up of following algae and content thereof preferably:
Chlorella 5 * 10 8~8 * 10 9Individual cell count/milliliter.
3, in the such scheme, in order further to improve effect, best bacterium phase preparation is made up of following bacterial classification and content thereof:
Photosynthetic bacterium 1 * 10 9~1 * 10 10Individual cell count/milliliter;
Yeast 5 * 10 7~1 * 10 8Individual cell count/milliliter;
Subtilis 8 * 10 7~2 * 10 8Individual cell count/milliliter;
Milk-acid bacteria 5 * 10 7~1 * 10 8Individual cell count/milliliter;
Best algae phase preparation is made up of following algae and content thereof:
Chlorella 1 * 10 9~5 * 10 9Individual cell count/milliliter.
4, bacterium phase preparation of the present invention is based on photosynthetic bacterium, and yeast, milk-acid bacteria and subtilis are auxilliary, and effect is to purify water, and removes the harmful chemical component in the water; Used algae phase preparation is a chlorella, is to be used for keeping water quality, heavy metal in the absorption water and unnecessary nutritive substance.The concrete mechanism of action and effect are described as follows:
(1), photosynthetic bacterium
The photosynthetic bacteria cell body constitutes element and mainly contains: carbon, hydrogen, oxygen, nitrogen, phosphorus, potassium, sodium, magnesium, calcium, sulphur and some trace elements etc., they also are the essential substance that all biomass cellss constitute.Generally speaking, cell fresh weight: water accounts for 80%-90%, inorganic salt 1%-1.5%, protein 7%-10%, fatty 1%-2%, carbohydrate and other organism 1%-1.5%.Wherein stem cell carbon containing 45%-55%, hydrogen 5%-10%, oxygen 20%-30%, nitrogen 5%-13%, phosphorus 3%-5%, other mineral element 3%-5%.Photosynthetic bacterium is mainly used in sea farming, freshwater aquiculture and poultry, egg poultry raising, disease-resistant crops volume increase etc.Wherein main particularly outstanding with the effect of aquaculture, be a kind of nuisanceless, the ecological ideas product has obtained the attention of the State Scientific and Technological Commission and the Ministry of Agriculture in recent years.State Planning Commission, economic and commercial committee have issued in " industry of current state key encourage growth, product and technology catalogue " recently, in animal husbandry and fishery, just comprised " ecologic breeding in the aquaculture " this.
1., in the aquaculture original traditional sterilizing method more harm than good.As use lime disinfection, unslaked lime is chloride, product is sour, produce sulphur, influence organic matter decomposition.Use chlorinated lime, the high more effect of water temperature is poor more, and PH is high more, and effect is poor more.Use potassium permanganate, as using under alkalescence and slightly acidic environment, Manganse Dioxide brown precipitation is deposition easily, and portion is influential to the fishes and shrimps gill, and respiratory tract infection easily takes place.In a word, use the chemicals sterilization and disinfection, it is all influential that fishes and shrimps are grown, not good to the disease resistance effect, and unwanted bacteria also can develop immunity to drugs simultaneously, makes the sterilization effect of chemicals more and more undesirable, and also more and more refractory is managed.
2., the effect of photosynthetic bacterium in aquatic products
A, purify water
Because contain a large amount of fish faeces and residual bait in the water body of high-density aquiculture, and the residue of fish medicine, ammonia-state nitrogen, hydrogen sulfide and some objectionable impuritiess that their corrupt backs produce, directly polluted-water and bed mud.Slight pollution can cause the fish life uncomfortable, feed coefficient increases, poor growth, after running up to a certain degree, can make under the water bottom anoxybiotic situation, photosynthetic bacterium (PSB) can absorb objectionable impuritiess such as ammonia-state nitrogen, hydrogen sulfide effectively, form thalline itself, simultaneously, after the formation advantage group, fish just can not poison or be dead.The eutrophication of water body also can be grown a large amount of pathogenic micro-organisms, makes the fish infection morbidity.After using PSB,, purify water, make the purpose of healthy fish growth thereby reach suppressing other growth of pathogenic bacteria.
B, safeguard the water body microecological balance
Exist various microorganisms in the water body of aquafarm, some is useful, and some is deleterious; Also some is cry " opportunistic microorganism " of the state of mediating, and promptly under the normal circumstances, this quasi-microorganism is not pathogenic, meets water pollution, and during the fish immunity function reduction, their just a large amount of breedings endanger fish.
Usually people adopt disinfectant to control, but increase along with application times, the resistance of pathogenic micro-organism also correspondingly strengthens, in order to reach the effect of prevention and treatment, the dosage of at every turn using has to strengthen gradually, and this has not only increased drug cost, but also has polluted water body, cause aquatic product quality to descend, even can not eat.
How to control the growth and breeding of pathogenic micro-organism, it is not developed immunity to drugs, polluted-water not? answer is ready-made, prevent fish disease with photosynthetic bacterium, can overcome the shortcoming of disinfectant fully, it is degradable or remove the harmful chemical that comprises the fish medicine in the water body not only, the photosynthetic bacterium that has comparative advantage also can fight for nutrition, space with pathogenic micro-organism, make it can't carry out the raised growth breeding, also there is not resistance simultaneously, thereby be difficult for to form morbific envrionment conditions, fish are susceptible disease not just also.
Photosynthetic bacterium is a beneficial bacteria, has the animal digestion of promotion and absorbs, and stimulating growth is grown, and improves immunologic function, suppresses various germ submerged special efficacys.Photosynthetic bacterium has special effect to improving water quality, and photosynthetic bacterium is sprinkled in the with serious pollution pond, and generally after 3 hours, it is clear that water quality begins to change, and went to see that compare with the pond of closing bacterium that do not black out, water quality was as far apart as heaven and earth, and knows at a glance in second day on every side.If fish disease is very serious, dead fish surface is all arranged every day, then used photosynthetic bacterium after, went to see that the dead fish of surface significantly reduced in second day, up to disappearance, and form sharp contrast on every side.With photosynthetic bacterium, also can help the fishes and shrimps safe overwintering effectively, the not only not underproduction in winter has volume increase on the contrary.With the fishery products of photosynthetic bacterium, bright-colored, individual neat, oppress fresh and tender! After 10 times of photosynthetic bacterium dilutions, fishes and shrimps are carried out dipping, the fishes and shrimps surviving rate is reached more than 90%, the bacteriosis, rotted gill disease surviving rate of being clamminess reaches 60~100%, fish molds, red fin fish disease, the sick surviving rate of scratch reach nearly 100%, compare than other chemicals, more safe and reliable, there is not any dipping side effect.With the general per mu yield raising of photosynthetic bacterium 15-23%, feed coefficient decline 18-23%, surviving rate improves 20-60%, individual body weight gains 15%, and input-output ratio reaches more than 1: 10.Every mu increases benefit and reaches more than 800 yuan.
(2), milk-acid bacteria
Milk-acid bacteria (LAB, Lactic acid bacteria) but be the general designation that a class can produce the bacterium of a large amount of lactic acid from fermentable carbohydrates (mainly referring to glucose), this class bacterium of having found at present comprises 18 genus at least on systematic bacteriology, mainly contain: lactobacillus genus (Lactobacillus), genus bifidobacterium (Bifidobacterium), streptococcus (Streptococcus), bright beading Coccus (Leuconostoc), enterococcus spp (Enterococcus), lactococcus Lactococcus), carnivorous Bacillaceae (Carnobacterium), unusual Pseudomonas (Atopobium), Pediococcus (Pediococcus), Aerococcus (Aerococcus), roaming Coccus (Vagococcus), listeria spp belongs to (Listeria), Sporolactobacillus (Sporolactobacilus), minority kind in the bacillus (Bacillus), circumfili Pseudomonas (Brochothrix), erysipelothrix (Erysipelothrix), twin Pseudomonas (Gemella) and sugared Coccus (Saccharococcus) etc.
The milk-acid bacteria overwhelming majority is anerobe or amphimicrobian chemotrophy bacterium, Gram-positive.Growth and breeding is in the abundant slightly acidic environment of anaerobism or little aerobic, mineral substance and organic nutrient substance.
As feed additive, milk-acid bacteria effect in actual applications is remarkable, and over the past two years, more research work concentrated in the discussion of mechanism of action of milk-acid bacteria performance function.Relevant report is a lot, combines that it is described, its mechanism of action mainly contain following some: 1) provide nutritive substance, have short body growth effect.2) improve micro-ecological environment, cleaning enteron aisle toxic substance.3) regulate digestion immunity system etc.
If milk-acid bacteria can normally be brought into play metabolic activity in vivo, just can directly provide available indispensable amino acid (as Methionin and methionine(Met) etc.) and various VITAMIN (vitamin B complex and K, H etc.) for the host, also can improve the biologic activity of mineral element, closely be reached for the host essential nutritive substance is provided, strengthen the Nutrition and Metabolism of animal, directly the effect of short its growth.
Because in the lactobacter growth metabolic process, can produce the material that some have antimicrobial acivity, as: organic acid, hydrogen peroxide, carbonic acid gas etc. are all at the external bacteriostatic activity that shows.A lot of milk-acid bacterias can both produce bacteriocin, as: nisalpin, lactobacillin is bitten sour rhzomorph etc., and these materials are suppressing to have vital role on the pathogenic bacteria after deliberation, therefore have important germicidal action in the process of purifying water.
(3), subtilis
Improve the animal intestinal micro-ecological environment, promote immunity system to grow, activate the non-specific immunity system, the enhance immunity function; Control livestock gastrointestinal tract disease also can be prevented and treated the pig bloody flux, diseases such as pig scrotitis; Increase day weight gain, improve surviving rate and efficiency of feed utilization, shorten breeding cycle, reduce feeding cost; Can quicken the proteinic deposition of animal body, promote lipid metabolism, improve lean ratio and livestock product quality; Anti-nutrient molecule in the degraded feed reduces chyme viscosity in the enteron aisle, improves the digestive utilization ratio of nutritive substance; Promote the absorption of nutritive substance, reduce the generation of poisonous and harmful corrupt substance in the enteron aisle, reduce the damage of intestines wall and the pollution of environment; Improve the water quality in the aquaculture, reduce ammonia nitrogen and nitrite in the water, improve water surrounding, improve output.After subtilis enters livestock and poultry alimentary tract with the spore state, bring back to life by dormant state at once, bring back to life oxygen in the bacterium mass consumption intestines, local oxygen molecule concentration is descended, can reduce intestinal bacteria in the enteron aisle significantly, the quantity of Salmonellas increases beneficial microorganism in the body intestinal tube and causes the disease microorganism and reduce, thereby purified the inside and outside environment, reduced the generation of disease; Produce hydrogen oxide, inhibitory substance such as bacteriocin can suppress the growth of putrefactive bacterium in the intestines, reduce the activity of urase, and then reduce the generation of objectionable impuritiess such as oxygen, amine, help the healthy growth of animal; Have stronger proteolytic enzyme, amylase and lipase activity, the enzyme that also has complex carbohydrates in the degrading plant feed simultaneously, enzymes such as pectin, dextran, Mierocrystalline cellulose, wherein a lot of enzymes are enzymes that Mammals and fowl do not have, thereby can improve food conversion ratio greatly; Synthetic vitamin B group.Subtilis is not before entering livestock and poultry alimentary tract, be to be in dormant state with the spore state, therefore insensitive for high temperature, in the pelletization of feed, can not be damaged, little ecologic effect in digestive tube can make the antibiotic dosage in the feed descend greatly in addition.
(4), yeast
Yeast aerobic anaerobic can both be lived.Produce part alcohol during anaerobic.Unnecessary organism in the water bodys such as yeast decomposition glucose produces carbon dioxide.Help the growth of other anerobes.
(5), chlorella
Chlorella (Chlorella Vulgaris) belongs to Chlorophyta (Chlorophyta).Experimental result in the past shows that the chlorella rich in protein is about 50~55%, nutritive value is equivalent to 5 times of egg, and 2 times of Semen arachidis hypogaeae are described as " pork in the water " by people, its vitamin contents than-as vegetables all high, have higher nutritive value and pharmaceutical use; The chlorophyll and DNA, the RNA that have the occurring in nature extra best best; Contain abundant mineral substance and important lipid acid.It can be used as good fodder additives and improving of environment agent, under the eutrophic water environment, can absorb unnecessary nitrogen, phosphorus nutrition, keeps the water body cleaning.
In a word, nutrition water adjusting agent of the present invention is by screening for many years, testing, through the refining compound micro-ecological preparation that is rich in multiple high reactivity probiotics and feed algae that forms of modern biotechnology.Product viable cell content height, stability is strong, reproduction speed is fast, can continue to improve water body environment, regulate water colour, reduce change that water causes stress.Improve substrate, increase water nutrition element and dissolved oxygen content, stablize water, promote water body to form benign cycle, keep bacterium phase, the algae phase eubiosis.Especially be applicable to the water quality improvement of water quality deterioration, body eutrophication, the foul cultivating pool of water colour.Can replenish the growth of mutually promoting mutually between the mixed bacterium, and culture condition is all similar.
The main effect of mixed bacterium:
1), decompose residual bait, ight soil, reduce toxic substances such as nitrite, ammonia nitrogen, hydrogen sulfide, improve water quality, regulate water colour.
2), increase the food consumption of cultivated animals such as fish, shrimp, promote nutritive substance to transform and absorb, promote growth.Reduce feed coefficient, and improve meat and local flavor.
3), cultivate beneficial microorganism, suppress pathogenic microorganism, stablize water, the optimum eubiosis of maintenance aquaculture water.Strengthen the immunizing power of aquatic animals such as fish, shrimp, crab, reduce " stress ".
4), culture and to cooperate early stage other rich water products to use, can accelerate rich water speed, cultivate good water colour and set up the beneficial microorganism population, improve survival rate of young such as fish, shrimp, crab.
The main effect of chlorella:
After mixed bacterium was with purification of water quality, the adding of chlorella can be kept water quality, regulated water pH value to neutral, and as the additive food of fish, shrimp, crab, finally formed the ecological circulation of a harmony.
Table 1 microbiological indicator:
Project Index (content in every milliliter of water purification agent)
Photosynthetic bacterium ≥10 9CFU
Yeast ≥10 8CFU
Subtilis ≥10 8CFU
Milk-acid bacteria 10 8CFU
Chlorella ≥10 8ind/ml
Other indexs of table 2:
Project Index
PH 6.2
Specific conductivity 2.0ms
TDS 998mg/L
Table 3 sanitary index:
Project Index
Arsenic, mg/kg ≤0.7
Lead, mg/kg ≤1.0
Coliform, individual/100g ≤40
Salmonellas Must not detect
Embodiment
Below in conjunction with embodiment the present invention is further described:
Embodiment:
1, a kind of nourishing water transfer agent for aquiculture, by bacterium phase preparation and algae mutually preparation two portions phase-splitting assembly constitute, wherein:
Bacterium phase preparation is made up of following bacterial classification and content thereof:
Photosynthetic bacterium 1 * 10 9~1 * 10 10Individual cell count/milliliter;
Yeast 5 * 10 7~1 * 10 8Individual cell count/milliliter;
Subtilis 8 * 10 7~2 * 10 8Individual cell count/milliliter;
Milk-acid bacteria 5 * 10 7~1 * 10 8Individual cell count/milliliter;
Algae phase preparation is made up of following algae and content thereof:
Chlorella 1 * 10 9~5 * 10 9Individual cell count/milliliter.
2, the preparation method of water adjusting agent:
In the laboratory, we are by bacterial classification purifying and screening, obtained fast growth, tolerance is strong, and the excellent species that absorbed dose is big is by homemade nutrient medium shaking culture (nutritive medium comprises: fish meal, protein powder, phosphoric acid salt, nitrate, trace element etc.), finally obtain four kinds of bacterium of a large amount of suitable production, equally, chlorella also is the good algae kind that obtains by the strictness screening, cultivates for aquatic No. 6 with green algae nutrient salt.During product preparation, four kinds of bacterium separately are cultured to earlier 1~10% of desired concn, and packing is preceding 6~10 days then, are mixed in proportion together and cultivate.When the concentration of bacterium reaches requirement by the time, divide the capacity of installing to be (consumption is a 2L/ mu) in the bottle of 2L, stand-by.Chlorella and bacterium also are to separate to cultivate, reach concentration requirement after, (consumption is tight 1L/ mu) is stand-by in the bottle of the 1L that independently packs into.
3, using method
(1), directly splash: every 2L mixed bacterium can be with 1 mu of water body (1 meter of the depth of water), and per about 15 days once.If with ratio and the clean water mixing of mixed bacterium with 1: 9, add one jin of brown sugar again and fully dissolve, to splash after in 25 ℃-35 ℃ environment, placing 12~18 hours, effect can be better.After 3-5 time, water quality purifies fully substantially, and the chlorella suspension 5~10L that splashes again gets final product.
(2), fine morning result of use the best, preferably start oxygenating machine during use.
(3), bait is mixed and is fed: by 100 jin of feed mixed bacterium 2L.Specific practice is: bacterium liquid is sprayed on the feed surface, fully mixes thoroughly, place and throw something and feed after 1-2 hour, once a day, use continuously.
Here it is emphasized that: when bacterium and algae were used simultaneously, the bacterium algae splashed into the pond with 10: 1 mixed.Remaining algae is taken out and puts into vat continuation cultivation; Bacterium and algae are splashed after 3~4 times, every 4~5 days, with the disposable pond that splashes into of remaining algae.
The main application of chlorella:
For the first time drop into aquaculture water simultaneously with four kinds of bacterium, effect is: absorb a large amount of carbonic acid gas that bacterium produces when the performance function, through photosynthesis fast, produce oxygen, prevent that fishery products such as fish, shrimp, crab are because of water hypoxia wan even death.At this moment be with the ratio that cooperates of other bacterium---photosynthetic bacterium: yeast: subtilis: milk-acid bacteria: chlorella=10: 1: 1: 1: 1 (cell count), and this is theoretic best proportioning, in fact, bacterium and algae all are constantly to breed.
Use separately for the second time, concentration is 1 * 10 9~1 * 10 10Individual cell count/milliliter.Because the PH of four kinds of bacterium can reach 2.5~5.0, makes water quality be weak acid even strongly-acid, the time weak point can play the effect of killing harmful bacterium of curing the disease, but the time has been grown the flesh of fish qualitative change and has sent, the mouthfeel variation, also dangerous to the existence of fry, influence output and sales volume.And pure chlorella PH is 9~10, belongs to alkalescence, and its adding can be in harmonious proportion water body PH, makes whole water body be neutrality substantially.The carbonic acid gas that bacterium produces is absorbed by algae, the quick merisis of algae, emit a large amount of oxygen, improve the water body oxygen level, simultaneously owing to itself contain high protein, and be individual less single-cell algae, be fit closely natural fish, shrimp feed therefore, and industry feed that fish, shrimp etc. do not eat up and movement are absorbed by bacterium and decompose.In secular breeding process, bacterium, algae and fishery products are in running balance all the time, keep water quality, improve the output and the quality of fishery products.
The foregoing description only is explanation technical conceive of the present invention and characteristics, and its purpose is to allow the personage who is familiar with this technology can understand content of the present invention and enforcement according to this, can not limit protection scope of the present invention with this.All equivalences that spirit is done according to the present invention change or modify, and all should be encompassed within protection scope of the present invention.

Claims (3)

1, a kind of nourishing water transfer agent for aquiculture is characterized in that: by bacterium phase preparation and algae mutually preparation two portions phase-splitting assembly constitute, wherein:
Bacterium phase preparation is made up of following bacterial classification and content thereof:
Photosynthetic bacterium 5 * 10 8~5 * 10 10Individual cell count/milliliter;
Yeast 1 * 10 7~5 * 10 8Individual cell count/milliliter;
Subtilis 1 * 10 7~1 * 10 9Individual cell count/milliliter;
Milk-acid bacteria 1 * 10 7~5 * 10 8Individual cell count/milliliter;
Algae phase preparation is made up of following algae and content thereof:
Chlorella 1 * 10 8~1 * 10 10Individual cell count/milliliter.
2, nourishing water transfer agent for aquiculture according to claim 1 is characterized in that:
Bacterium phase preparation is made up of following bacterial classification and content thereof:
Photosynthetic bacterium 8 * 10 8~3 * 10 10Individual cell count/milliliter;
Yeast 3 * 10 7~3 * 10 8Individual cell count/milliliter;
Subtilis 5 * 10 7~5 * 10 8Individual cell count/milliliter;
Milk-acid bacteria 3 * 10 7~3 * 10 8Individual cell count/milliliter;
Algae phase preparation is made up of following algae and content thereof:
Chlorella 5 * 10 8~8 * 10 9Individual cell count/milliliter.
3, nourishing water transfer agent for aquiculture according to claim 1 is characterized in that:
Bacterium phase preparation is made up of following bacterial classification and content thereof:
Photosynthetic bacterium 1 * 10 9~1 * 10 10Individual cell count/milliliter;
Yeast 5 * 10 7~1 * 10 8Individual cell count/milliliter;
Subtilis 8 * 10 7~2 * 10 8Individual cell count/milliliter;
Milk-acid bacteria 5 * 10 7~1 * 10 8Individual cell count/milliliter;
Algae phase preparation is made up of following algae and content thereof:
Chlorella 1 * 10 9~5 * 10 9Individual cell count/milliliter.
CNB200510041478XA 2005-08-09 2005-08-09 Nourishing water conditioning agent for aquiculture Expired - Fee Related CN100494093C (en)

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