CN104556406B - Utilize the method that microorganism and chlorella carry out efficient in-situ immobilization to lake sewage - Google Patents
Utilize the method that microorganism and chlorella carry out efficient in-situ immobilization to lake sewage Download PDFInfo
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- CN104556406B CN104556406B CN201410846797.7A CN201410846797A CN104556406B CN 104556406 B CN104556406 B CN 104556406B CN 201410846797 A CN201410846797 A CN 201410846797A CN 104556406 B CN104556406 B CN 104556406B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
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- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The present invention relates to a kind of method utilizing microorganism and chlorella that lake sewage is carried out efficient in-situ immobilization.It comprises the following steps: 1, original inhabitants' beneficial microorganism separates and purification, qualification, conservation；2, the original inhabitants' separation of chlorella algae, qualification: in the different location of lake region, gathers lake water, examines under a microscope the kind of Micro Algae, uses capillary tube partition method separation chlorella, conservation；3, according to fluctuating temperature and the pH value feature of lake Various Seasonal, the growth preferable beneficial bacteria kind of step 1 isolated under configuration corresponding conditions, and carry out the large-scale culture of multiple bacterial strain；The chlorella that step 2 separates conservation carries out, in large-scale culture, the beneficial microbe then large-scale culture obtained and chlorella importing lake, carrying out the purification of lake sewage.Its indigenous beneficial microbe based on lake separation and Culture and chlorella carry out the in-situ immobilization of lake sewage, have the effect that efficiency is high, with strong points.
Description
Technical field
The invention belongs to environmental technology field, be specifically related to the in-situ remediation method of a kind of lake sewage.
Background technology
The self-purification capacity that lake is the most certain, can utilize the microorganism of himself, unit cell algae, water plant etc. raw
Thing oneself purify water, and keeps water quality pure and fresh.In recent years, due to a large amount of discharges of rural sewage, sanitary sewage etc., such as raise pigs
The sewage that field produces, causes the eutrophication of water body, bottom water body severe depletion of oxygen, greatly inhibits the work of aerobic microbiological
Property.Anaerobe amount reproduction under anaerobic condition, carries out anaerobic digestion to the Organic substance falling into the bottom, produce hydrogen sulfide,
The harmful gas such as methane, ammonia, and then poison the fish and shrimp put in a suitable place to breed in water.Hydrogen sulfide reacts with the ferrum in water again, generates iron sulfide and makes
Water body turns black.The organic deposition that can not get decomposing in time, at the bottom, forms black mud.Additionally, due to water Middle nutrition salt group
The change become, causes the change of water body algae phase, usually breaks out cyanophyceae, cause catastrophic consequence.Accordingly, it would be desirable to mass propgation has
Beneficial microorganism and useful unit cell algae, the pollutant in degradation water fast and efficiently, the outburst of suppression cyanophyceae.Thus can avoid
Throw and spill medicine, non-secondary pollution.There are substantial amounts of beneficial microbe and useful Micro Algae series products, these products on the market at present
There is several subject matter.First, strain and algae kind are degenerated serious, and product quality is uneven, and using effect is unstable；The
Two, product Techniques of preserving falls behind, and some products are after dispatching from the factory in short-term, and microorganism and algae vigor decline rapidly, not even
To 10% when dispatching from the factory；3rd, administer water body in lake big, commercial prod price is higher, and large area use cost is high；4th, no
Big with lake water quality indicator difference, market product cannot be made to measure for the water quality specificity in certain lake, and using effect is poor
Different greatly.Thus, it is provided that the efficient restorative procedure of a kind of lake sewage is extremely necessary.
Summary of the invention
It is an object of the invention to provide and a kind of utilize microorganism and chlorella that lake sewage is carried out efficient in-situ immobilization
Method, its indigenous beneficial microbe based on lake separation and Culture and chlorella carry out the in-situ immobilization of lake sewage, have effect
The effect that rate is high, with strong points.
For solving above-mentioned technical problem, the technical solution used in the present invention is as follows:
Thering is provided a kind of method utilizing microorganism and chlorella that lake sewage is carried out efficient in-situ immobilization, it includes following
Step:
1, original inhabitants' beneficial microorganism separates and purification, qualification, conservation: in the different location of lake region, gathers eutrophication
Bed mud, add sterilizing distilled water, sediment, shake up, the float after shaking up is based on different types of bacteria resistant
Property difference it is carried out treatment of different temperature after be seeded on agar culture medium, carry out bacterial strain routine screening, identify bacterial strain kind
Class, purification and conservation, the bacterial species of noxious substance in degradation water that has then screening obtained carries out optimal growth condition
Test, it is thus achieved that its best growing condition；
2, the original inhabitants' separation of chlorella algae, qualification: in the different location of lake region, gathers lake water, examines under a microscope list
The kind of born of the same parents algae, uses capillary tube partition method separation chlorella, conservation；
3, according to fluctuating temperature and the pH value feature of lake Various Seasonal, step 1 isolated under configuration corresponding conditions
Grow preferable beneficial bacteria kind, and carry out the large-scale culture of multiple bacterial strain；The chlorella that step 2 separates conservation is carried out
Large-scale culture, the beneficial microbe then large-scale culture obtained and chlorella import in lake, carry out lake sewage
Purify.
By such scheme, the heating-up temperature in described step 1 is 4-70 degree Celsius, is the 2-of bottom mud in lake by water quality
3 times.
By such scheme, described in there is the bacterial species of noxious substance in degradation water include but not limited to bacillus category
Group, nitrite bacteria monoid, nitrobacteria monoid and enterococcus monoid.
By such scheme, described the best growing condition includes optimum growth temp and optimal pH etc..
By such scheme, in described step 3, the culture medium prescription used by bacterial strain large-scale culture is: in 1 cubic metre of water body:
0.5-1 kilogram of yeast extract powder, peptone 1-2 kilogram, industry rock salt 1-2 kilogram.
By such scheme, in described step 3, the culture medium prescription used by chlorella large-scale culture is: 1 cubic metre of water body
In: ammonium hydrogen carbonate: 300-500 gram, phosphate fertilizer: 30-50 gram.
Beneficial effects of the present invention:
The inventive method can screen beneficial microbe and the chlorella obtaining noxious substance in degradable water pointedly, enters
One step can configure according to the fluctuating temperature of lake Various Seasonal and pH value feature and grow preferable beneficial bacteria kind under corresponding conditions
Class and chlorella, carry out large-scale culture, and then can be based on from lake situ isolated original inhabitants beneficial microbe and bead
Algae, effectively purifies lake water quality；With low cost, it is one of the percentage of current market price；It is easy to promote.
Detailed description of the invention
Below in conjunction with example, the summary of the invention of the present invention is further described:
Embodiment 1: Pig farm wastewater causes the purification of water quality of freshwater lake eutrophication
Certain 2000 mu of lake, owing to the sewage not processed is discharged for a long time to lake in neighbouring pig farm, causes pig farm sewage draining exit
The main water quality deterioration in neighbouring 50 meters of regions, index is: ammonia nitrogen 26.1mg/L；Dissolved oxygen 1.83mg/L；Biochemical oxygen demand
44.1mg/L；Float 76mg/L.According to country's water quality standard for fishery, These parameters should be: ammonia nitrogen is less than 0.02mg/L；Molten
Solve oxygen more than 3mg/L；Biochemical oxygen demand is less than 5mg/L；Float is less than 10mg/L, These parameters and standard water-quality ratio after blowdown
As follows: ammonia nitrogen exceeds standard 1305 times;Dissolved oxygen is the 61%(anoxia of standard);Biochemical oxygen demand exceeds standard 8.82 times;Float surpasses
Mark 7.6 times.Due to water quality deterioration, there is the mortality of cultured fishes in plant, and kind mainly has Aristichthys nobilis, silver carp, Carassius auratus, group
Head triangular bream and Cyprinus carpio, it is seen that dead fish adds up to more than 220,000 jin, loss at least 1,100,000 RMB.
Use following in-situ remediation method that lake sewage is repaired:
1, original inhabitants' beneficial microorganism separates and purification, qualification, conservation: in the different location of lake region, gathers eutrophication
Bed mud, add sterilizing distilled water, sediment, shake up, the float after shaking up is based on different types of bacteria resistant
It is carried out being seeded on agar culture medium after the process of different temperatures in 4-70 degree Celsius range by the difference of property, carries out bacterial strain normal
Rule screening, identifies bacterial strain kind, purification and conservation, and specifically, during discrimination of bacteria, on picking agar culture medium, single bacterium colony is in liquid
In culture medium, 28 DEG C of shaking tables are cultivated 24 hours, do bacterium solution PCR and expand 16S rDNA and check order.Round pcr: primer sequence: 27F:
5’-AGAGTTTGATCCTGGCTCAG-3’；1492R:5 '-TACGGCTACCTTGTTACGACTT-3 '.Reaction system: bacterium solution 1
μ L, 27F:1 μ L(10 μM), 1492R:1 μ L(10 μM), 2 × Taq Mix:10 μ L, dd H2O:7 μ L.Response procedures: pre-
Degeneration 95 DEG C, 5 min；Degeneration 95 DEG C, 30 s, anneal 55 DEG C, 40 s, extend, 72 DEG C, 2 min, 35 circulations；Terminate extending
72 DEG C, 10 min.The 16srDNA PCR fragment obtained is checked order, comparison, it is thus achieved that the classification information of microorganism.With
Time to obtain microorganism carry out physiological and biochemical index qualification, determine its kind.Separate and obtain bacillus subtilis, solve starch bud
Spore bacillus, enterococcus faecalis, Nitromonas, Nitrosomas, these then screening obtained have the thin of noxious substance in degradation water
Strain class carries out growth conditions test, it is thus achieved that its optimal growth conditions.The suitable growth temperature of various antibacterials and pH value range are such as
Lower bacillus subtilis (15-45 DEG C, pH:6.0-8.8), bacillus amyloliquefaciens (13-46 DEG C, pH:6.0-8.5), excrement intestinal ball
Bacterium (10-40 DEG C, pH:6.0-8.5), Nitromonas (15-38 DEG C, pH:6.5-7.5), Nitrosomas (13-38 DEG C, pH:6.6-
7.7)；
2, the original inhabitants' separation of chlorella algae, qualification: in the different location of lake region, gathers lake water, examines under a microscope list
The kind of born of the same parents algae, uses capillary tube partition method separation chlorella, conservation；
3, according to fluctuating temperature situation and the pH value feature of lake Various Seasonal, under configuration corresponding conditions, step 1 separates
The growth preferable beneficial bacteria kind arrived, carries out the large-scale culture of multiple bacterial strain in following culture medium.In water temperature 10-25
DEG C time, bacteria combination is enterococcus faecalis, Nitromonas and Nitrosomas；When water temperature 25-37 DEG C, bacteria combination be enterococcus faecalis,
Nitromonas, Nitrosomas, bacillus subtilis and bacillus amyloliquefaciens.Described culture medium prescription is: in 1 cubic meter of water: ferment
Female 0.5-1 kilogram of extractum powder, peptone 1-2 kilogram, industry rock salt 1-2 kilogram；Step 2 is separated the chlorella of conservation under
Stating and carry out large-scale culture in culture medium, the beneficial microbe then large-scale culture obtained and chlorella import in lake,
Carrying out the purification of lake sewage, described culture medium prescription is: in 1 cubic meter of water: ammonium hydrogen carbonate: 300-500 gram, phosphate fertilizer: 30-
50 grams.
Being continuously injected into cultured antibacterial and chlorella through half a year, water standard reaches ammonia nitrogen 0.01mg/L；Dissolved oxygen
3.5mg/L；Biochemical oxygen demand 4.3 mg/L；Float 8mg/L, reaches water quality standard for fishery.
Claims (2)
1. one kind utilizes the method that microorganism and chlorella carry out efficient in-situ immobilization to lake sewage, it is characterised in that: its bag
Include following steps:
(1), original inhabitants' beneficial microorganism separates and purification, qualification, conservation: in the different location of lake region, gather eutrophication
Bed mud, adds the distilled water of sterilizing, sediment, shakes up, and the float after shaking up is based on different types of bacterial resistance
Difference it is carried out treatment of different temperature after be seeded on agar culture medium, carry out bacterial strain routine screening, identify bacterial strain kind,
Purification and conservation, the bacterial species of noxious substance in degradation water that has then screening obtained carries out optimal growth condition examination
Test, it is thus achieved that its best growing condition；The described treatment temperature in step (1) is 4-70 degree Celsius, is at the bottom of lake by water quality
2-3 times of mud；Described have the bacterial species of noxious substance in degradation water and include but not limited to that bacillus cereus monoid, nitrosation are thin
Mushroom group, nitrobacteria monoid and enterococcus faecalis monoid；
(2), the original inhabitants separation of chlorella algae, qualification: in the different location of lake region, gather lake water, examine under a microscope unit cell
The kind of algae, uses capillary tube partition method separation chlorella, conservation；
(3), according to fluctuating temperature and pH value feature, step (1) isolated under configuration corresponding conditions of lake Various Seasonal
Growing preferable beneficial bacteria kind, and carry out the large-scale culture of multiple bacterial strain, when water temperature 10-25 DEG C, bacteria combination is
Enterococcus faecalis, Nitromonas and Nitrosomas；When water temperature 25-37 DEG C, bacteria combination be enterococcus faecalis, Nitromonas, Nitrosomas,
Bacillus subtilis and bacillus amyloliquefaciens；The chlorella that step (2) separates conservation carries out large-scale culture, then will be big
Beneficial microbe and chlorella that scale evaluation obtains import in lake, carry out the purification of lake sewage；
In described step (3), the culture medium prescription used by bacterial strain large-scale culture is: in 1 cubic meter of water: yeast extract powder
0.5-1 kilogram, peptone 1-2 kilogram, industry rock salt 1-2 kilogram；
In described step (3), the culture medium prescription used by chlorella large-scale culture is: in 1 cubic metre of water body: ammonium hydrogen carbonate:
300-500 gram, phosphate fertilizer: 30-50 gram.
The method utilizing microorganism and chlorella that lake sewage is carried out efficient in-situ immobilization the most according to claim 1,
It is characterized in that: described the best growing condition includes optimum growth temp and optimal pH.
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| CN105858904A (en) * | 2016-06-12 | 2016-08-17 | 江西鸿吉生物科技有限公司 | Bioremediation nutritional agent for water balance system and preparing method thereof |
| CN107686815B (en) * | 2017-10-17 | 2020-05-22 | 中国水产科学研究院渔业机械仪器研究所 | Method for cultivating chlorella by using food waste |
| CN110002600B (en) * | 2019-02-28 | 2022-06-03 | 南京华创环境技术研究院有限公司 | Culture method of microbial colony, in-situ culture device and wastewater treatment method |
| CN113307383A (en) * | 2021-06-28 | 2021-08-27 | 重庆工商大学 | Activated microorganism activation and propagation system and method for sewage treatment |
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