CN105985917B - Method for increasing biomass of chlorella in pig-raising wastewater - Google Patents
Method for increasing biomass of chlorella in pig-raising wastewater Download PDFInfo
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- CN105985917B CN105985917B CN201510063872.7A CN201510063872A CN105985917B CN 105985917 B CN105985917 B CN 105985917B CN 201510063872 A CN201510063872 A CN 201510063872A CN 105985917 B CN105985917 B CN 105985917B
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Abstract
The invention provides a method for improving biomass of chlorella in pig-raising wastewater, belonging to the field of microorganism application. According to the invention, the chlorella with the preservation number of CGMCC NO.9225 and the paenibacillus with the preservation number of CGMCC NO.7724 are co-cultured in the pig-raising wastewater, and the result shows that the paenibacillus can obviously improve the biomass of the chlorella in the pig-raising wastewater, and the promotion effect is 1.6 times. The method solves the problem of pig raising wastewater treatment, reduces environmental pollution, improves the water body microenvironment in the pig raising wastewater by adding the paenibacillus, obviously improves the chlorella biomass, reduces the production cost of the microalgae biodiesel, obtains better economic benefit and has good market application prospect.
Description
Technical Field
The invention belongs to the field of microorganism utilization and biological grease preparation, and particularly relates to a method for improving biomass of chlorella in pig wastewater.
Background
the oil-rich microalgae industry has become one of the most promising biodiesel industries and is receiving much attention. Compared with other energy crops, microalgae has multiple advantages, such as low investment and high yield, no land competition with agriculture, no competition with agriculture for fertilizer, growth of waste gas, waste water and saline water, no need of herbicide and pesticide, and microalgae cell residue can be used for agricultural fertilizer or animal feed or continuous production of biogas or ethanol.
At present, the pollution of animal excrement is more severe, which not only causes water eutrophication and causes harm to agricultural production, but also has negative influence on the living environment of people. With the increasing environmental and energy problems, the combination of microalgae culture and sewage treatment has become a new trend. The pig-raising wastewater (LSM) contains a large amount of pig excreta, is rich in organic carbon sources and other nutrient components such as nitrogen and phosphorus, is regarded as high-concentration sewage, and can cause water eutrophication and water source pollution when directly discharged. In addition, the pig raising wastewater carries a large amount of pathogenic bacteria, emits extremely strong odor and increasingly causes serious pollution to the environment. Microalgae have the ability to take advantage of organic and inorganic contaminants in wastewater and convert them into cellular components (e.g., lipids and carbohydrates). Microalgae are high-quality raw materials for producing biodiesel. The oil yield of the microalgae is 30-100 times that of oil crops such as soybeans, and a foundation is laid for producing alternative fuel oil. The algae uses light energy as energy and utilizes nutrient substances such as nitrogen, phosphorus and the like to synthesize complex organic matters. Therefore, the algae can reduce the content of nitrogen and phosphorus in the sewage. Has good effect on removing nitrogen and phosphorus in the sewage.
However, the selection of algal species is of crucial importance, and only specific algal species can grow in swine wastewater. This is mainly due to the ammonia Nitrogen (NH) in the pig-raising wastewater3-N) are too high and contain other toxic substances, to which most microalgae are very sensitive, thus limiting the growth of microalgae in the wastewater. Previous studies have shown that only a small fraction of the Chlorella sp, Scenedesmus sp and Euglena sp can be used in sewage treatment. In addition, although some algal species can grow in swine wastewater, their biomass is low and oil content is low, which is not economical relative to the production of biodiesel. It can be said that,Two breakthroughs are needed for the microalgae to generate more biodiesel in sewage, one is to select a proper algae species and a proper culture method to increase the biomass of the microalgae, and the other is to increase the oil content in microalgae cells.
Research shows that the microalgae can provide oxygen and organic matters needed by the heterotrophic bacteria, and meanwhile, carbon dioxide and nutrients (micromolecular nitrogen and phosphorus) released by the heterotrophic bacteria can be used for the growth of the microalgae.
The nitrogen fixing bacillus belongs to self-growing nitrogen fixing bacteria, and is a microorganism which can produce spores, has strong stress resistance, long survival time, strong adaptability and wide distribution and can fix nitrogen. The nitrogen-fixing bacillus has the nitrogen-fixing function, and can generate substances for promoting plant growth and antibacterial substances, so that the soil microenvironment can be effectively improved, the plant growth can be promoted, and the disease resistance of the plants can be enhanced.
Disclosure of Invention
The invention aims to provide a method for improving biomass of chlorella in pig-raising wastewater.
The invention firstly provides a method for improving the biomass of Chlorella (Chlorella sp.) which is to add Paenibacillus (Paenibacillus sp.) into a culture medium for culturing the Chlorella.
The invention provides a method for improving biomass of Chlorella (Chlorella sp.) in pig-raising wastewater, which is characterized in that Paenibacillus (Paenibacillus sp.) is added into the pig-raising wastewater for culturing the Chlorella.
In the method, the Chlorella is 34-2, the preservation number is CGMCC NO.9225, the strain is preserved in China general microbiological culture Collection center (CGMCC for short, the address is No. 3 of West Lu No. 1 of Beijing city Ind. district, microbiological research institute of Chinese academy of sciences, postal code 100101) in 5, 15 days in 2014, and the strain is classified and named as Chlorella sp.
The Paenibacillus is 18WLY, the preservation number is CGMCC NO.7724, the strain is preserved in the common microorganism center of China Committee for culture Collection of microorganisms (CGMCC for short, the address: No. 3 of West Lu No. 1 of Beijing Kogyo Ind area, Microbiol research institute of China academy of sciences, postal code 100101) in 19 th 6 th 2013, and the strain is classified and named as Paenibacillus sp.
the pig raising wastewater is obtained by diluting wastewater generated by raising pigs in a pig farm by 3-6 times of volume. Preferably, the wastewater obtained after dilution by 5 times. The diluent is selected from tap water or deionized water. The purpose of dilution is to provide a better water body microenvironment for the chlorella and enable the chlorella to be more fully subjected to light for photosynthesis.
In the method for improving the biomass of Chlorella (Chlorella sp.) in the pig-raising wastewater, provided by the invention, the initial inoculation amount of the Chlorella is 3 multiplied by 108-5×108Each/ml of pig raising wastewater; the initial inoculation amount of Paenibacillus is 1 × 10-4-2×10-4g/ml pig raising wastewater.
Preferably, the initial inoculum size of chlorella is 4.3 × 108Each/ml of pig raising wastewater; the initial inoculation amount of Paenibacillus is 1.26X 10-4g/ml pig raising wastewater.
Furthermore, in the method, the dry weight ratio of the inoculated paenibacillus to the inoculated pediococcus is 15-20: 1. Preferably, the dry weight ratio of paenibacillus to pediococcus is 18: 1.
The invention co-cultures Paenibacillus and Chlorella in pig-raising wastewater under the culture conditions of 20-35 ℃, the ratio of light to dark in one day is 14-18:6-10, and the culture lasts for 3-8 days. Preferably, the culture is carried out at 28 ℃ for 3 days with a light to dark time ratio of 16:8 in one day. The illumination can be natural light or artificial light source, and the growth promotion effect is obvious when the illumination intensity is 5000 lux.
The method can be applied to preparation of the biological grease.
The invention also provides the Paenibacillus sp with the preservation number of CGMCC NO.7724 and the application of the microbial inoculum containing the Paenibacillus sp in improving the biomass of Chlorella sp.
The invention provides a Paenibacillus sp with a preservation number of CGMCC NO.7724 and application of a microbial inoculum containing the same in reducing wastewater pollution of a farm.
The Paenibacillus sp 18WLY (preservation number CGMCC NO.7724) is a nitrogen-fixing bacillus strain screened by the applicant in the previous period, has the characteristics of strong adaptability and strong nitrogen fixing capability, and can effectively promote the yield improvement of crops such as corn and the like. The applicant unexpectedly finds that after the strain and Chlorella sp.34-2 are co-cultured in pig-raising wastewater, the Paenibacillus sp.18WLY plays a remarkable promoting role in biomass after the growth of the Chlorella sp.34-2 is transiently inhibited, and the promoting role is as high as 1.6 times. This result and its use are unpredictable by those skilled in the art in the field of microalgae biomass energy.
the invention also provides a method for producing the biological oil, which co-cultures Chlorella (Chlorella sp.)34-2 and Paenibacillus (Paenibacillus sp.)18WLY in pig farm wastewater.
The Chlorella sp.34-2 is collected from Xuan city of Anhui province, and has the characteristic of rapid growth in non-sterilized pig-raising wastewater (the average growth rate of the dry weight of algae cells is 165 mg.L after being cultured in the pig-raising wastewater for 10 days-1·d-1) And can convert carbon source pollutants in the sewage into biological grease of which the concentration is up to 30 percent, and has good settleability and easy collection. In addition, the chlorella has high removal efficiency on nutrient elements such as carbon, nitrogen, phosphorus and the like in the pig raising wastewater, and the removal rates of nitrogen and phosphorus in the pig raising wastewater after 7 days of treatment are respectively 60% and 85%. Moreover, the algae has strong environmental adaptability, can grow well under the environment of 20-40 ℃ and the pH range of 3-11, and has larger production and application potential. The chlorella and Paenibacillus sp are co-cultured in the pig farm wastewater for the first time, the Paenibacillus sensation is found to generate a remarkable promoting effect on the improvement of the biomass of the chlorella, and the biomass of the chlorella in the stationary phase reaches 1.80 multiplied by 1010The biomass of chlorella in a bottle (1.15 multiplied by 10) is expressed as the biomass of non-inoculated Paenibacillus sp.18WLY101.6 times of the total weight per ml), the growth promoting effect is obvious. The method solves the problem of pig-raising wastewater treatment, reduces environmental pollution, and improves the addition of the paenibacillusThe method has the advantages of obviously improving the water microenvironment in the pig-raising wastewater, obviously improving the biomass of the chlorella, reducing the production cost of the microalgae biodiesel, obtaining better economic benefit, having good market application prospect and protecting the ecology.
Drawings
FIG. 1 is a graph showing the growth of Chlorella vulgaris 34-2.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are commercially available products.
Example 1 measurement of Components of wastewater from pig farming and preparation of diluted wastewater from pig farming
The pig wastewater in this example comes from Beijing Changying Zhen village macro breeding farm. The method for measuring the Total Nitrogen (TN) of the pig-raising wastewater is an alkaline potassium persulfate digestion ultraviolet spectrophotometry according to the national environmental protection standard HJ 636-2012 of the people's republic of China; the method for measuring Total Phosphorus (TP) is ammonium molybdate spectrophotometry according to the national standard GB118WLY93-93 of the people's republic of China. Ammonia Nitrogen (NH)3-N) and COD are determined according to Lianhua water quality rapid detection instruments 5B-6C. The TN and NH of the pig-raising wastewater are determined by the determination method3N, TP and COD components, the results of the measurements are shown in Table 1:
TABLE 1 determination of pig-raising wastewater composition
Adding tap water into the pig raising wastewater to dilute the pig raising wastewater to 5 times of the volume of the original supernatant, and subpackaging the diluted pig raising wastewater in 250ml clean conical bottles, wherein each bottle contains 100ml of the diluted pig raising wastewater. The sewage after being diluted by adding tap water comprises the following components by weight percent: TN is about 121 mg.L-1，NH3-N is about 104 mg-ml-1TP of about 16.4 mg.L-1COD was about 332 mg.L-1。
Example 2 method for increasing the biomass of Chlorella in pig wastewater
1. Separation, purification and culture of chlorella and free-living azotobacter
(1) Separation, purification and culture of chlorella: solid plate TAP Medium: 1.5% of agar powder; chlorella sp.34-2 single clones on plates were picked and plated by streaking for storage and purification of algal species, subcultured once per week.
A single clone was picked from a TAP plate of Chlorella sp.34-2 and inoculated into 20ml of TAP liquid medium, and then 1ml of the culture was inoculated into 100ml of TAP liquid medium and placed in a 25 ℃ light incubator for static culture.
(2) Separation, purification and culture of Paenibacillus 18 WLY: solid LB medium: 1.5% of agar powder; selecting a monoclonal from Paenibacillus sp.18WLY, inoculating the monoclonal on a plate by a streaking method for storing and purifying strains, and subculturing once every three days
A single clone is selected from an LB plate of Paenibacillus sp.18WLY and inoculated into 20ml of LB culture medium, then 1ml of culture solution is taken into 100ml of LB liquid culture medium, and the culture medium is placed into a shaking table at 28 ℃ for shaking culture, and the rotating speed of the horizontal shaking table is 150 rpm.
2. Co-culture of Paenibacillus 18WLY and Chlorella vulgaris 34-2
The pure Paenibacillus sp.18WLY culture solution and the pure Chlorella sp.34-2 culture solution which are in logarithmic phase are respectively centrifuged for 10min at the temperature of 4 ℃ and the rpm of 4000 to obtain cell precipitates. The cell pellet was washed 3 times with sterile deionized water to remove residual media components.
Adding a proper amount of sterilized water according to the amount of the cell sediment for suspension, uniformly mixing by using a vortex oscillator to prepare concentrated solution, respectively inoculating Chlorella sp and paenibacillus sp.18WLY into 100ml of sterilized diluted pig-raising wastewater in the example 1 according to the table 2, and adding the sterilized water to ensure that the final volumes of the liquids in all the bottles are the same. Controlling the algae cell inoculation amount by using a microscope and a red blood cell counting plate to ensure that the inoculated Chlorella sp.34-2 is 4.3 multiplied by 1087X 10 units/ml-6g/ml (dry weight); the Paenibacillus sp.18WLY of the Paenibacillus is 1.26 multiplied by 10- 4g/ml (dry weight). The dry weight ratio of the inoculated paenibacillus to the inoculated chlorella is 18: 1.
TABLE 2 initial inoculum size of each bottle
The bottles are placed into a 25 ℃ illumination incubator for culture, and the day mode is 5000lux for 16h, and the night mode is 8 h. Observing every 24h, comparing the growth condition difference, and counting the number of microalgae cells in each group by using a blood counting plate. The fermentation was terminated after 6 days of co-cultivation.
Culture observation shows that the bottles in the blank group and the bacteria group inoculated with only Paenibacillus sp.18WLY have no obvious color change and have no green change, which is caused by the fact that Chlorella sp.34-2 is not inoculated in the bottles. While the negative control group inoculated with Chlorella only sp.34-2 (i.e., group 3) and the positive control group inoculated with both Paenibacillus sp.18WLY and Chlorella sp.34-2 (i.e., group 4) were green. And since day 3 after inoculation, the positive control group showed a darker green color than the negative control group, indicating that the number of microalgae cells was greater in the positive control group than in the negative control group.
Counting the microalgae cells in each bottle by using a microscope and a red blood cell counting plate every 24 hours. Counting results are subjected to calculation statistics and growth curves are drawn for comparison, and the results are shown in figure 1. As shown in FIG. 1, the positive control group had a long lag phase during the growth of Chlorella, but had a shorter log phase and a significantly higher biomass increase in the log phase than the negative control group that had not been inoculated with Paenibacillus sp.18WLY, while the positive control group had a biomass of Chlorella of 1.80X 10 at the stationary phase10One/ml, as Chlorella biomass in negative control group (1.15 × 10)101.6 times of the total weight per ml), the growth promoting effect is obvious.
The results show that the Paenibacillus sp.18WLY can remarkably promote the biomass of Chlorella sp.34-2 in the pig-raising wastewater, and the reason of the results can be related to that the Paenibacillus improves the microenvironment of the water body in which the microalgae is located.
Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (6)
1. A Chinese medicinal preparation for improving chlorellaChlorella sp.) method for producing biomass in swine wastewater, characterized in that a Paenibacillus (Bacillus sp.) is added to swine wastewater containing chlorellaPaenibacillussp.), the chlorella is 34-2, the preservation number is CGMCC NO.9225, the paenibacillus is 18WLY, and the preservation number is CGMCC NO. 7724;
The pig raising wastewater is obtained by diluting the wastewater generated by raising pigs in a pig farm by 3-6 times.
2. the method of claim 1, wherein the initial inoculum size of chlorella is 3 x 108-5×108Each/ml of pig raising wastewater; the initial inoculation amount of Paenibacillus is 1 × 10-4-2×10-4g/ml pig raising wastewater.
3. The method of claim 1, wherein the dry weight ratio of inoculated paenibacillus to pediococcus is 15-20: 1.
4. The method of claim 1, wherein the culturing is carried out under conditions of 20-35 ℃ and a light to dark time ratio of 14-18:6-10 during a day for 3-8 days.
5. Use of the method of any one of claims 1 to 4 for the preparation of a biolipid.
6. Paenibacillus with preservation number of CGMCC NO.7724 (Paenibacillussp.) and microbial inoculum containing the same in improving pelletAlgae (1)Chlorella sp.) in the pig-raising wastewater, wherein the chlorella is 34-2, and the preservation number is CGMCC NO. 9225; the pig raising wastewater is obtained by diluting the wastewater generated by raising pigs in a pig farm by 3-6 times.
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| CN107080045A (en) * | 2017-05-25 | 2017-08-22 | 甘肃凯源生物技术开发中心 | A kind of healthy pig feed of the cultivation containing chlorella and preparation method |
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| CN109609382B (en) * | 2018-12-07 | 2022-03-22 | 中国石油大学(华东) | Method for promoting growth of chlorella and oil accumulation by algal-bacteria co-culture |
| CN109402025A (en) * | 2018-12-12 | 2019-03-01 | 河西学院 | A kind of both culturing microalgae prevention and cure of pollution method |
| CN110699258B (en) * | 2019-11-18 | 2021-09-24 | 江苏师范大学 | Culture method for improving chlorella cell biomass |
| CN110982859B (en) * | 2019-12-23 | 2023-05-05 | 哈尔滨工业大学 | Method for producing carbohydrate by utilizing pig raising sewage |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010103199A (en) * | 2000-05-04 | 2001-11-23 | 복성해 | Bioflocculant Produced by Paenibacillus polymixa KCTC 0766BP for Microalgae Harvesting |
| CN101735966A (en) * | 2009-09-25 | 2010-06-16 | 中国科学院南海海洋研究所 | Strong bacillus G25-1-2 and applications thereof |
| CN102093955A (en) * | 2010-12-03 | 2011-06-15 | 福建漳州鼎能生物科技有限公司 | Chlorella strain and application thereof |
| CN102168053A (en) * | 2011-02-13 | 2011-08-31 | 南京农业大学 | Paenibacilluspolymyxa strain for coproduction of LI-F type antibiotic and dibutyl phthalate and application thereof |
| CN102352332A (en) * | 2011-10-21 | 2012-02-15 | 中国农业大学 | Nitrogen fixing Paenibacillus 1-43 and application thereof |
| CN102352331A (en) * | 2011-10-21 | 2012-02-15 | 中国农业大学 | Nitrogen-fixing Paenibacillus sp. 1-33 and application thereof |
| CN102391978A (en) * | 2011-12-12 | 2012-03-28 | 西南大学 | Aloe endophytic bacterium with broad-spectrum antibacterial activity and colonization capability in animal intestinal tract |
| CN102557766A (en) * | 2012-02-21 | 2012-07-11 | 浙江大学 | Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water |
| WO2012109221A1 (en) * | 2011-02-07 | 2012-08-16 | E. I. Du Pont De Nemours And Company | Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms |
| CN102642929A (en) * | 2012-05-01 | 2012-08-22 | 复旦大学 | Artificial light source adjusting method utilizing chlorella to purify pig manure waste water |
| CN103451130A (en) * | 2013-07-25 | 2013-12-18 | 中国农业大学 | Nitrogen fixing gene cluster and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9113605B2 (en) * | 2009-11-20 | 2015-08-25 | Core Intellectual Properties Holdings, Llc | Methods and compositions to aggregate algae |
| US9428425B2 (en) * | 2012-09-20 | 2016-08-30 | Core Intellectual Properties Holdings, Llc | Methods and compositions for treating soil and plants |
-
2015
- 2015-02-06 CN CN201510063872.7A patent/CN105985917B/en not_active Expired - Fee Related
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010103199A (en) * | 2000-05-04 | 2001-11-23 | 복성해 | Bioflocculant Produced by Paenibacillus polymixa KCTC 0766BP for Microalgae Harvesting |
| CN101735966A (en) * | 2009-09-25 | 2010-06-16 | 中国科学院南海海洋研究所 | Strong bacillus G25-1-2 and applications thereof |
| CN102093955A (en) * | 2010-12-03 | 2011-06-15 | 福建漳州鼎能生物科技有限公司 | Chlorella strain and application thereof |
| WO2012109221A1 (en) * | 2011-02-07 | 2012-08-16 | E. I. Du Pont De Nemours And Company | Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms |
| CN102168053A (en) * | 2011-02-13 | 2011-08-31 | 南京农业大学 | Paenibacilluspolymyxa strain for coproduction of LI-F type antibiotic and dibutyl phthalate and application thereof |
| CN102352332A (en) * | 2011-10-21 | 2012-02-15 | 中国农业大学 | Nitrogen fixing Paenibacillus 1-43 and application thereof |
| CN102352331A (en) * | 2011-10-21 | 2012-02-15 | 中国农业大学 | Nitrogen-fixing Paenibacillus sp. 1-33 and application thereof |
| CN102391978A (en) * | 2011-12-12 | 2012-03-28 | 西南大学 | Aloe endophytic bacterium with broad-spectrum antibacterial activity and colonization capability in animal intestinal tract |
| CN102557766A (en) * | 2012-02-21 | 2012-07-11 | 浙江大学 | Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water |
| CN102642929A (en) * | 2012-05-01 | 2012-08-22 | 复旦大学 | Artificial light source adjusting method utilizing chlorella to purify pig manure waste water |
| CN103451130A (en) * | 2013-07-25 | 2013-12-18 | 中国农业大学 | Nitrogen fixing gene cluster and application thereof |
Non-Patent Citations (5)
| Title |
|---|
| 《Cp-S316菌株抗细菌活性物质对大肠杆菌的抑制作用及高产突变株选育》;杨少波等;《微生物学通报》;20090620;第36卷(第6期);参见对比文件2引文3摘要 * |
| 《FISH检测多粘类芽孢杆菌研究及其在猪粪有机肥和土壤中的应用》;张秋霞;《南京农业大学硕士学位论文》;20120630;参见对比文件2第1页第1-2段 * |
| 《Harvesting of Chlorella vulgaris using a bioflocculant from Paenibacillus sp. AM49》;Hee-Mock Oh1等;《Biotechnology Letters》;20011231;第23卷;全文 * |
| 《利用芽孢菌发酵废水培养小球藻的研究》;武玉强等;《生 物 学 通 报》;20131130;第48卷(第11期);全文 * |
| 《基于小球藻培养的初级猪粪废水净化与油脂生产耦合技术》;牟晓庆;《中南民族大学硕士学位论文》;20130331;参见对比文件1摘要、第24-25页、参见对比文件1摘要、第26、35-36页 * |
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