CN102557766A - Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water - Google Patents
Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water Download PDFInfo
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Abstract
The invention discloses an application of a Paenibacillus macerans strain in the dissolution of phosphorus sources in soil or eutrophic water. The Paenibacillus macerans strain is Paenibacillus macerans ZJU0902 with a preservation number of CCTCC NO:M209158; the phosphorus source is at least one of inorganic phosphorus sources and organic phosphorus sources. According to the invention, Paenibacillus macerans ZJU0902 is used for the dissolution of inorganic and organic phosphorus sources in soil, and has the characteristics of stability, high efficiency, and wide spectra for phosphorus dissolution; After a microbial fertilizer prepared by mixing the bacterial strain fermentation broth with a fertilizer matrix is applied to soil, dominant microflora are formed rapidly; the fertilizer efficiency is good; the effective phosphorus content of the soil can be improved significantly; and the healthy growth of plants is promoted; the bacterial strain is used for the dissolution of inorganic and organic phosphorus sources in eutrophic water, can promote the absorption of superfluous phosphorus element in water by water plants, and thus prevent and control water eutrophication.
Description
Technical field
The present invention relates to microbial technology field, relate in particular to the application in a kind of Paenibacillus macerans bacterial strain phosphorus source in dissolving soil or eutrophication water.
Background technology
Plant-growth needs some essential nutritive elements, and phosphorus is wherein a kind of principal element that can influence the plant health growth.Phosphorus is not only the component of many important compound in the plant materials, but also with the intravital important metabolism of number of ways involved in plant.But 90% phosphorus all exists with the indissoluble state in the soil, and this possibly be because the phosphorus in the natural soils is prone to and Fe under acidic conditions
3+, Al
3+The plasma complexing forms FePO
4And AlPO
4Deng difficultly-soluble phosphates; Under alkaline condition again with Ca
2+Ion complexation forms Ca
3(PO
4)
2Or CaHPO
4Etc. inertia phosphoric acid salt, these phosphorus all can not directly be absorbed by plant, thereby hinder the healthy growth of plant, and make output can not get maximization.
Body eutrophication is meant the water pollution phenomenon that plant nutrient substance content such as nitrogen, phosphorus too much cause, the result causes that aquatic ecosystem forfeiture oneself keeps, the ability of self-control, causes the further aggravation of environmental pollution.According to statistics, China's eutrophication and ultra eutrophic lake reach 66% and 22% (silk floss waits .2010 well recklessly) of lake total amount respectively.Improving waterplant the receptivity of inorganic elementss such as nitrogen, phosphorus is prevented and treated body eutrophication, is an important subject in current environmental protection field.
Exist some mikrobes in the soil and can the phosphate transfection of insoluble be turned to form capable of using, these mikrobes be called phosphate solubilizing microorganism (phosphate-solubilizing microorganisms, PSM).Phosphate solubilizing microorganism can rely on the meta-bolites of self or dissolve indissoluble inorganic phosphorus or organophosphorus in soil with other biological synergetic, thereby in the plain conversion of phosphorus, plays an important role.Therefore the research of phosphate solubilizing microorganism receives scientist's attention always, and expectation improves the validity of soil insoluble phosphorus and the utilising efficiency of phosphate fertilizer with the approach of Inoculant.Because phosphorus decomposing ability between different types of phosphate solubilizing bacteria or the bacterial strain and effect of inoculation difference great disparity are so the screening of efficient phosphorus decomposing bacterial strain seems particularly important.Though the phosphorus decomposing ability of fungi is greater than bacterium, the phosphate-solubilizing bacteria proportion is far above fungi in microbial fertilizer is produced.Separate at present phosphorus stabilizer in the microbial fertilizer production, bacterial strain efficient, wide spectrum is also fewer, this has proposed urgent task (Fan BQ, 2002) for the seed selection work of strain excellent.
Paenibacillus macerans is split into (Guemori-Athmani et al.2000 from the rhizosphere of a lot of plants; Von der Weid et al.2000).Research shows, soaks crudefiber crop gemma bar and belongs to that bacterium is heat-resisting, stable, natural adaptation power is strong, is not only short living bacterium (the Artursson et al.2006 of plant-growth at present; Or biocontrol microorganisms (the Timmusk et al.2009 of plant opposing multiple diseases Algam et al.2010); Li et al.2007,2010,2011a, b).Veritably, Paenibacillus macerans has come to light and has been involved in plant growth-promoting (Timmusk et al.1999) and germ inhibition (Bae et al.2000; Timmusk 2003; Li et al.2011a, b), but at present also not about the report of Paenibacillus macerans as the phosphorus dissolution of bacteria.Therefore, the phosphorus dissolving power of research Paenibacillus macerans is for clearly short life of this schizomycete and diseases prevention mechanism have very important significance.
Summary of the invention
The invention provides the application in a kind of Paenibacillus macerans bacterial strain phosphorus source in dissolving soil or eutrophication water, this Paenibacillus macerans bacterial strain can stablize, efficiently, wide spectrum ground dissolves the inorganic or organophosphorus source in soil or the eutrophication water.
The application in a kind of Paenibacillus macerans bacterial strain phosphorus source in dissolving soil or eutrophication water, described Paenibacillus macerans bacterial strain is Paenibacillus macerans (Paenibacillus macerans) ZJU0902, preserving number is CCTCC NO:M 209158; Described phosphorus source is at least a in inorganic phosphorous sources and the organophosphorus source.
Described Paenibacillus macerans ZJU0902 preservation is preserved in the Chinese typical culture collection center (CCTCC) that is positioned at Lopa Nationality an ancient woman's ornament mountain, Wuhan City Wuhan University, and preservation date is on July 19th, 2009, and deposit number is CCTCC NO:M 209158.Publication number is to disclose this bacterial strain in the application for a patent for invention of CN101712942A; And its screening, cultural method, form and biological property etc. are disclosed in detail; This bacterial strain is to separate in the topsoil around the cucumber rhizosphere in the serious cucumber ground that takes place of the bacterial wilt in Hangzhou, Zhejiang province city to obtain; Through being accredited as Paenibacillus macerans, plant-bacterial-wilt had certain control effect.
Described soil can be Ca
3(PO
4)
2, CaHPO
4, FePO
4Or AlPO
4Higher Deng insoluble phosphate content, and need the more weak soil of phosphorus plant growing way.
Described need the phosphorus plant can be the plant very responsive to phosphorus such as crop in cruciferae, leguminous crop, tomato, eggplant or beet, perhaps also can need the phosphorus plant for corn, sesame etc. are medium.
Described eutrophication water can be for being rich in the eutrophication water of phosphoric.
Described inorganic phosphorous sources can be Ca
3(PO
4)
2, CaHPO
4, FePO
4And AlPO
4In one or more mixtures.
Preferably, described inorganic phosphorous sources is Ca
3(PO
4)
2Or CaHPO
4
Described organophosphorus source can be phytic acid.
Described application can be passed through following steps: activated Paenibacillus macerans ZJU0902 is inoculated in the LB liquid nutrient medium, in 28-30 ℃ of cultivation 12-24h, makes seed liquor; Seed liquor is added in soil or the eutrophication water, mix;
Wherein, in every kilogram of soil or every liter of eutrophication water, the add-on of said seed liquor is 5-10mL.
Analyze the phosphorus decomposing ability of bacterial strain for ease, can Design Laboratory phosphorus decomposing experimental study bacterial strain to the dissolving of P source compound.
The phosphorus decomposing test of described laboratory can be passed through following steps: Paenibacillus macerans ZJU0902 is inoculated in the NBRIP liquid nutrient medium that is added with the phosphorus source, cultivates 1-7d in 28 ℃ of shaking tables;
Wherein, in volume 1L, described NBRIP liquid nutrient medium comprises following component: glucose 10g, and magnesium chloride hexahydrate 5g, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.25g, Repone K 0.2g, ammonium sulfate 0.1g, surplus is a water, pH 7.0; The addition in said phosphorus source is counted 0.001-0.04mol with every liter of NBRIP liquid nutrient medium.
Described NBRIP liquid nutrient medium is a substratum commonly used in the mikrobe phosphorus decomposing research, and it is relatively sensitiveer that this substratum detects phosphorus content.
Described shaking table incubation time is preferably 4-6d; 5d more preferably, the phosphorus decomposing effect is best.
The present invention also provides a kind of microbial fertilizer that can dissolve the soil phosphorus source, and described microbial fertilizer contains following composition: cell concentration is greater than 10
8The Paenibacillus macerans bacterial strain of individual/g, mass concentration are the full phosphorus that the full nitrogen of 4-6%, organic matter that mass concentration is 35-40% and mass concentration are 1-2%;
Wherein, described Paenibacillus macerans bacterial strain is Paenibacillus macerans ZJU0902, and preserving number is CCTCC NO:M 209158;
Contain the organonitrogen that mass concentration is 90%-100% in the described full nitrogen.
Described microbial fertilizer can impose in the soil of plantation crop in cruciferae, leguminous crop, tomato, eggplant, beet, corn or sesame.
The present invention also provides the preparation method of mentioned microorganism fertilizer, comprising: Paenibacillus macerans ZJU0902 is inoculated in the LB enriched medium, in 28-30 ℃ of cultivation 24-48h, obtains fermented liquid; Fermented liquid is added in the base-material by weight the ratio of 4-6%, and mixing in 20-28 ℃ of fermentation 3-5d, makes microbial fertilizer;
Wherein, described LB enriched medium makes by adding ammonium nitrate, sulphur ammonium and analysis for soybean powder in the LB liquid nutrient medium;
Described base-material is one or more mixtures in feces of livestock and poultry, crop material, inorganic nitrogenous fertilizer and the bean dregs.
Preferably, described microbial fertilizer also can be mixed and made into by Paenibacillus macerans ZJU0902 fermented liquid and organic fertilizer with high nitrogen.The preparation of said organic fertilizer with high nitrogen can be with reference to the relevant record in the patent of invention of publication number CN1215022C.
Among the present invention, described Paenibacillus macerans ZJU0902 separates from plant rhizosphere soil and obtains, and it can adapt to complicated edatope; Simultaneously, laboratory phosphorus decomposing test and plant test-results show, this bacterial strain has dissolving power to multiple inorganic or organophosphorus source, and hence one can see that, and this bacterial strain can be used for the dissolving in soil phosphorus source.On the other hand; Test according to the laboratory phosphorus decomposing; Paenibacillus macerans ZJU0902 is good to phosphorus source solute effect in the NBRIP liquid nutrient medium, shows that this Paenibacillus macerans ZJU0902 can be used for dissolving the phosphoric of liquid environment, like the dissolving in phosphorus source in the eutrophication water.
The present invention is used for to separate the Paenibacillus macerans ZJU0902 obtain in the soil that soil is inorganic, the dissolving in organophosphorus source, has the advantages that to separate phosphorus stabilizer, efficient, wide spectrum, and helps the essential phosphoric of plant absorbing growth, promotes the growing way of plant; With this bacterial strain fermentation liquor and fertilizer matrix mixed microbial fertilizer, can form dominant microflora rapidly after being manured into soil, good fertilizer efficiency can make that available phosphorus content significantly improves in the soil, promotes the healthy growth of plant.This bacterial strain is used for that eutrophication water is inorganic, the dissolving in organophosphorus source, can impel waterplant to absorb unnecessary phosphoric in the water body, thus the control body eutrophication.
Description of drawings
Fig. 1 is the standard phosphorus curve of setting up with 50mg/l phosphoric acid standardized solution among the embodiment 1;
Fig. 2 is the size that Paenibacillus macerans ZJU0902 dissolves loop diameter among the embodiment 3 to different phosphate sources;
Fig. 3 be among the embodiment 5 inoculation time to the influence of Paenibacillus macerans ZJU0902 phosphorus dissolving power; Wherein, abcd representes the significant difference between different treatment;
Fig. 4 be among the embodiment 5 inoculation time to the influence of Paenibacillus macerans ZJU0902 thalli growth; Wherein, abcdef representes the significant difference between different treatment.
Embodiment
In following examples; Used Paenibacillus macerans (Paenibacillus macerans) ZJU0902 (is preserved in Chinese typical culture collection center; Deposit number is CCTCC M209158; The preservation time is on July 19th, 2009) screening, cultural method, form and biological property etc. be open in the application for a patent for invention of CN101712942A at publication number all, be specially:
This bacterial strain be in the serious cucumber ground that takes place of the bacterial wilt in Hangzhou, Zhejiang province city around the cucumber rhizosphere the topsoil separation screening obtain, be accredited as Paenibacillus macerans (Paenibacillus macerans) through Biolog, lipid acid and 16S rDNA.Biological characteristics is following: cell is shaft-like, and Gram-positive is moved with peritrichous.Oval gemma is arranged in the packing expanding, on nutrient agar medium, do not have soluble pigment.Amphimicrobian.Bacterium colony on substratum is thin, is the shape expansion, and bacterium is measured many and sticks mattness.Grower can stick and link on the substratum.28-30 ℃ of growth optimum temperuture, growth temperature range 15-50 ℃.The growth ph optimum is 7.0, and growth pH scope is 5.6-8.5.
With KH
2PO
4Dry 4h down at 105 ℃, accurately take by weighing 0.2195g KH
2PO
4Soluble in water and move on in the volumetric flask of 1000ml, add the 400ml water and the 5ml vitriol oil, constant volume obtains 50mg/l phosphoric acid standardized solution to 1000ml.As the phosphorus source, be made into the standardized solution of series concentration with 50mg/l phosphoric acid standardized solution, set up one about OD
450The typical curve of phosphorus content in value and the solution can be by the OD that is surveyed in the experiment through typical curve
450Value obtains corresponding phosphorus content.
Wherein, the mensuration of phosphorus content adopts the yellow light-intensity method of phosphorus molybdenum, is specially:
(1) take by weighing 2.4g NaOH, add water and constantly stirring, constant volume is made into 6N NaOH solution to 100ml;
Take by weighing 0.25g 2,4-dinitrophenol solid adds the 12.5ml absolute ethyl alcohol, and constant volume is made into 2 to 100ml then, 4-dinitrophenol indicator;
Take by weighing 0.5g (NH
4)
6MoO
24H
2O is dissolved in the 200ml zero(ppm) water, takes by weighing 0.625gNH
4Vo
3In 150ml boiling water, wait the cooling back to add the 125ml concentrated nitric acid, drop to room temperature; At leisure ammonium molybdate solution is poured in the ammonium metavanadate solution, constantly stirred, last constant volume is made into ammonium meta-vanadate and ammonium molybdate mixed solution to 500ml.
(2) the phosphoric acid standardized solution of series concentration is pipetted the 10ml supernatant in the 25ml volumetric flask after centrifugal; Splash into 12,4-dinitrophenol indicator dropwise adds 6N NaOH then and makes solution yellow just occur; Add ammonium meta-vanadate and ammonium molybdate mixed solution 5ml; Constant volume is at room temperature placed 15min to 25ml, surveys OD
450Value.
Paenibacillus macerans ZJU0902 with preservation rules on the NA agar plate with three zoning collimation methods, places 28 ℃ to cultivate 24h down; The single bacterium colony of Paenibacillus macerans ZJU0902 is received in the LB liquid nutrient medium, and in 28 ℃, shaking culture 12h processes seed liquor in the shaking table of 160r/min, and cell concentration is 10 in the seed liquor
8CFU/ml.
The mensuration of phosphorus dissolving power in embodiment 3 solid mediums
The NBRIP liquid nutrient medium (glucose 10g, magnesium chloride hexahydrate 5g, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.25g, Repone K 0.2g, ammonium sulfate 0.1g, zero(ppm) water 1000ml adds 5g/l inorganic phosphorus Ca respectively in pH7.0)
3(PO
4)
2, CaHPO
4, FePO
4And AlPO
4And 5ml/l organophosphorus phytic acid adds agar (20g/l) again and is mixed with solid medium as the phosphorus source.A kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices behind the autoclaving picks purifying is cultivated among the embodiment 2 Paenibacillus macerans ZJU0902 to flat board with the toothpick of bacterium of going out, 30 ℃ of unglazed cultivations.Detect the diameter of transparent phosphorus dissolving circle after 4 weeks.Repeat 4 times.
The result shows that Paenibacillus macerans ZJU0902 can dissolve Ca
3(PO
4)
2, CaHPO
4With the phosphoric in the phytic acid, and maximum to the phosphorus meltage of phytic acid.In solid medium, Paenibacillus macerans ZJU0902 can not dissolve FePO
4, AlPO
4In phosphoric (Fig. 2).
The mensuration of phosphorus dissolving power in embodiment 4 liquid nutrient mediums
In the 50ml Erlenmeyer flask, pour into 20ml NBRIP liquid nutrient medium (glucose 10g, magnesium chloride hexahydrate 5g, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.25g, Repone K 0.2g, ammonium sulfate 0.1g, zero(ppm) water 1000ml pH7.0), adds 5g/l inorganic phosphorus Ca respectively
3(PO
4)
2, CaHPO
4, FePO
4And AlPO
4And 5ml/l organophosphorus phytic acid is as the phosphorus source.It is 10 that Paenibacillus macerans ZJU0902 seed liquor among the inoculation embodiment 2 makes the bacterium starting point concentration
7CFU/ml cultivates 3d on 28 ℃, 130r/min shaking table, collect the centrifugal 10min of 10000r/min behind the bacteria suspension, adopts the OD of the yellow spectrphotometric method for measuring supernatant of phosphorus molybdenum among the embodiment 1
450The value, according to the standard phosphorus curve among the embodiment 1 with OD
450Value converts phosphorus content to.
The result shows that Paenibacillus macerans ZJU0902 can dissolve different inorganic phosphorus and organophosphorus, and to Ca
3(PO
4)
2, CaHPO
4, phytic acid the phosphorus dissolving power very strong.Though compare with separately contrast (not inoculating strain, other is identical), the phosphorus meltage all significantly increases, Paenibacillus macerans ZJU0902 is to Ca
3(PO
4)
2, CaHPO
4The phosphorus meltage increase 3-12 doubly, to FePO
4The phosphorus meltage increase more than 1 times, and to AlPO
4, phytic acid the phosphorus meltage increase minimum.This explanation bacterium is different to the phosphorus dissolving power of different phosphate sources, the most suitable dissolving of Paenibacillus macerans ZJU0902 Ca
3(PO
4)
2, CaHPO
4(seeing table 1).
Table 1 Paenibacillus macerans ZJU0902 is to the phosphorus dissolving power of different phosphate sources
Annotate: ab representes the significant difference between different treatment.
In the 50ml Erlenmeyer flask, pour into 20ml NBRIP liquid nutrient medium (glucose 10g, magnesium chloride hexahydrate 5g, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.25g, Repone K 0.2g, ammonium sulfate 0.1g, zero(ppm) water 1000ml pH7.0), adds 5g/l Ca
3(PO
4)
2As the phosphorus source.It is 10 that Paenibacillus macerans ZJU0902 seed liquor among the inoculation embodiment 2 makes the bacterium starting point concentration
7CFU/ml cultivates 1d on 28 ℃, 130r/min shaking table, 2d, and 3d, 4d, 5d, 6d, 7d collects the centrifugal 10min of 10000r/min behind the bacteria suspension respectively, adopts the OD of the yellow spectrphotometric method for measuring supernatant of phosphorus molybdenum among the embodiment 1
450The value, according to the standard phosphorus curve among the embodiment 1 with OD
450Value converts phosphorus content to.Measure the Paenibacillus macerans ZJU0902 cell quantity of corresponding time with colony counting method.
Result such as Fig. 3 and shown in Figure 4, the phosphorus content after the inoculation 2d in the solution reaches 21.8 μ g/ml, and 1d does not have significant difference with inoculation; Inoculation 2-3d during this period of time in, the maximum of advancing the speed of the phosphorus content in the solution, the phosphorus content after the inoculation 3d in the solution reaches 54.9 μ g/ml, is 2.5 times of inoculation 2d phosphorus content.The interior during this period of time phosphorus content of inoculation 3-5d slowly increases, and the phosphorus content after the inoculation 5d in the solution reaches peak 58.6 μ g/ml.Inoculation 5-7d, phosphorus content slightly descends, but does not have significant difference.Therefore inoculate 5d Paenibacillus macerans ZJU0902 the dissolving of calcium phosphate is reached peak, 5d is best inoculation time.It is inconsistent that the phosphorus content of this and Son et al. (2006) increases the report that descends with inoculation time, possibly cause owing to phosphorus dissolution of bacteria kind difference.
In addition, thalli growth is the result show, the thalline quantity after the inoculation 2d in the solution reaches peak, and than the initial 2.4log10 CFU/ml that increased, this has explained in inoculation back 2-3d during this period of time the phosphorus content maximum phenomenon of advancing the speed well.
Along with the increase of inoculation time, thalline quantity begins to reduce, and reaches 7.66 log10 CFU/ml to inoculation 7d cell quantity.
Embodiment 6 phosphate-solubilizing bacteria Paenibacillus macerans ZJU0902 degraded phosphorus source Ca
3(P0
4)
2Plant test
Preparation NBRIP liquid nutrient medium (glucose 10g, magnesium chloride hexahydrate 5g, MAGNESIUM SULPHATE HEPTAHYDRATE 99.5 0.25g, Repone K 0.2g, ammonium sulfate 0.1g, zero(ppm) water 1000ml, pH 7.0), add 5g/l Ca
3(PO
4)
2As the phosphorus source.Draw the 10ml nutrient solution in the petridish that is covered with 3 layers of sterilization filter paper.Cucumber seeds is soaked 4h in the Paenibacillus macerans ZJU0902 bacteria suspension of embodiment 2 preparation or sterile distilled water, transfer in the petridish, dark vernalization is 3 days in 16 hours 21 ℃/8 hours 19 ℃ of artificial culture casees, measures percentage of germination.Replenish the 5ml nutrient solution every day behind the seed germination, enough moisture arranged and can make therefrom Absorption of Phosphorus element of its root to guarantee the seed seedling.The results cucumber seedling is measured root, stem fresh weight after 10 days.Each handles 5 repetitions, and each repeats 80 seeds.
The result shows; The cucumber seeds percentage of germination of handling with Paenibacillus macerans ZJU0902 bacteria suspension reaches 87%; The cucumber seeds percentage of germination of handling with zero(ppm) water reaches 85%; This explanation Paenibacillus macerans ZJU0902 can not influence the normal germination of cucumber seeds, and the percentage of germination of cucumber seeds itself is just very high.In addition, can see that the cucumber seedling growing way of handling with Paenibacillus macerans ZJU0902 bacteria suspension is better than contrast from root, the stem fresh weight of cucumber seedling.This explanation Paenibacillus macerans ZJU0902 is with the Ca in the nutrient solution
3(PO
4)
2Be degraded into available phosphorus, promote the absorption of cucumber seedling phosphoric.
The table 2 Paenibacillus macerans ZJU0902 Ca that degrades
3(PO
4)
2Influence to plant germination and growth of seedling
Annotate: ab representes the significant difference between different treatment.
The production of embodiment 7 phosphate-solubilizing bacteria Paenibacillus macerans ZJU0902 microbial organic fertilizers
(1) Paenibacillus macerans ZJU0902 preparation of fermentation liquid: the seed liquor among the embodiment 2 is pressed inoculum size 5-10% (v/v); Rotating speed 130-160r/min; Culture temperature 28-30 ℃, incubation time 24-48h, (the LB substratum adds ammonium nitrate, sulphur ammonium, analysis for soybean powder etc. at fermention medium; PH 6.8-7.2) fermentation culture in obtains high density fermentation liquid (bacteria containing amount＞10
10Individual/ml).
(2) preparation of organic carrier: by Chinese patent " method and the fertilizer product thereof that utilize agricultural solid residue to process ", the patent No.: the ZL200410014773.1 disclosed method is produced organic fertilizer with high nitrogen, with the organic carrier of this as fertilizer sources.
(3) preparation of microbial fertilizer: fermented liquid is added in the organic carrier by weight 5% ratio, place 3-5d behind the thorough mixing under field conditions (factors), make moisture to the water cut of scattering and disappearing, promptly obtain the phosphate solubilizing microorganism organic fertilizer less than 30%.
Through measuring, bacterial number reaches 10 in the finished product
8Individual/more than the g, total nitrogen content reaches 4%, and wherein organonitrogen content reaches more than 90%, and organic content reaches 35%, and content of tatal phosphorus is 1-2%.Product index reaches the standard of national microorganism organic fertilizer.
Crop in cruciferae, leguminous crop, tomato, eggplant or beet etc. are the crops very responsive to phosphorus.Wherein rape, tomato Chang Zuowei lack phosphorus indication crop and are used to judge whether the field piece has competent available phosphorus.
In Hangzhou, ground such as Jinhua chooses phosphorus total content height and the low field piece of available phosphorus content, the microorganism organic fertilizer that imposes on present embodiment is executed, maize planting, cucumber plant, and contrast is set.Observe whole growth course, lack phosphorus milpa slight of stature, cauline leaf is tangible red-purple mostly, and filigree is extracted out late, female fringe deformity, and fringe is little, and setting percentage is low, postpones ripe.Cucumber lacks whole short and small the deadlock of strain of phosphorus, and dark green, it is shrivelled that sorrel appears in Lao Ye, the institute's cucumber color of tying Huang and little.And that the microorganism organic fertilizer of executing present embodiment is executed the corn and the cucumber plant growing way of planting on the block is good, and cauline leaf is luxuriant, grow normal, tie corn and cucumber yield obviously increases.
Claims (7)
1. the application in Paenibacillus macerans bacterial strain phosphorus source in dissolving soil or eutrophication water; It is characterized in that; Described Paenibacillus macerans bacterial strain is Paenibacillus macerans (Paenibacillus macerans) ZJU0902, and preserving number is CCTCC NO:M 209158; Described phosphorus source is at least a in inorganic phosphorous sources and the organophosphorus source.
2. application according to claim 1 is characterized in that, described inorganic phosphorous sources is Ca
3(PO
4)
2, CaHPO
4, FePO
4And AlPO
4In one or more mixtures.
3. application according to claim 2 is characterized in that, described inorganic phosphorous sources is Ca
3(PO
4)
2Or CaHPO
4
4. application according to claim 1 is characterized in that, described organophosphorus source is a phytic acid.
5. application according to claim 1 is characterized in that, comprises the steps: activated Paenibacillus macerans ZJU0902 is inoculated in the LB liquid nutrient medium, in 28-30 ℃ of cultivation 12-24h, makes seed liquor; Seed liquor is added in soil or the eutrophication water, mix;
Wherein, in every kilogram of soil or every liter of eutrophication water, the add-on of said seed liquor is 5-10mL.
6. the microbial fertilizer that can dissolve the soil phosphorus source is characterized in that, described microbial fertilizer contains following composition: cell concentration is greater than 10
8The Paenibacillus macerans bacterial strain of individual/g, mass concentration are the full phosphorus that the full nitrogen of 4-6%, organic matter that mass concentration is 35-40% and mass concentration are 1-2%;
Wherein, described Paenibacillus macerans bacterial strain is Paenibacillus macerans ZJU0902, and preserving number is CCTCC NO:M 209158;
Contain the organonitrogen that mass concentration is 90%-100% in the described full nitrogen.
7. the preparation method of a microbial fertilizer as claimed in claim 6 is characterized in that, comprising: Paenibacillus macerans ZJU0902 is inoculated in the LB enriched medium, in 28-30 ℃ of cultivation 24-48h, obtains fermented liquid; Fermented liquid is added in the base-material by weight the ratio of 4-6%, and mixing in 20-28 ℃ of fermentation 3-5d, makes microbial fertilizer;
Wherein, described LB enriched medium makes by adding ammonium nitrate, sulphur ammonium and analysis for soybean powder in the LB liquid nutrient medium;
Described base-material is one or more mixtures in feces of livestock and poultry, crop material, inorganic nitrogenous fertilizer and the bean dregs.
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| CN104293693A (en) * | 2014-07-14 | 2015-01-21 | 东华大学 | Method for preparing jute fiber by utilizing aneurinibacillus migulanus DY3 strain through retting |
| CN105985917A (en) * | 2015-02-06 | 2016-10-05 | 中国农业大学 | Method for improving biomass of chlorella in swine wastewater |
| CN106754371A (en) * | 2016-12-06 | 2017-05-31 | 广州富生源环保工程有限公司 | A kind of microbial bacterial agent preparation method and application for improving soil phosphorus utilization |
| CN108504586A (en) * | 2017-12-06 | 2018-09-07 | 四川明湖环保科技有限公司 | Soften series bacillus and straw decomposing inoculant |
| CN110643554A (en) * | 2019-11-26 | 2020-01-03 | 中国科学院烟台海岸带研究所 | Strain capable of efficiently removing inorganic phosphorus in water body and application thereof |
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| CN104277995A (en) * | 2014-07-14 | 2015-01-14 | 东华大学 | Aneurinibacillus migulanus DY3 strain and application thereof |
| CN104293693A (en) * | 2014-07-14 | 2015-01-21 | 东华大学 | Method for preparing jute fiber by utilizing aneurinibacillus migulanus DY3 strain through retting |
| CN104277995B (en) * | 2014-07-14 | 2017-01-11 | 东华大学 | Aneurinibacillus migulanus DY3 strain and application thereof |
| CN105985917A (en) * | 2015-02-06 | 2016-10-05 | 中国农业大学 | Method for improving biomass of chlorella in swine wastewater |
| CN105985917B (en) * | 2015-02-06 | 2019-12-13 | 中国农业大学 | Method for increasing biomass of chlorella in pig-raising wastewater |
| CN106754371A (en) * | 2016-12-06 | 2017-05-31 | 广州富生源环保工程有限公司 | A kind of microbial bacterial agent preparation method and application for improving soil phosphorus utilization |
| CN108504586A (en) * | 2017-12-06 | 2018-09-07 | 四川明湖环保科技有限公司 | Soften series bacillus and straw decomposing inoculant |
| CN108504586B (en) * | 2017-12-06 | 2020-05-05 | 四川明湖环保科技有限公司 | Paenibacillus softening and straw decomposing agent |
| CN110643554A (en) * | 2019-11-26 | 2020-01-03 | 中国科学院烟台海岸带研究所 | Strain capable of efficiently removing inorganic phosphorus in water body and application thereof |
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