CN102557766B - Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water - Google Patents
Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water Download PDFInfo
- Publication number
- CN102557766B CN102557766B CN2012100388003A CN201210038800A CN102557766B CN 102557766 B CN102557766 B CN 102557766B CN 2012100388003 A CN2012100388003 A CN 2012100388003A CN 201210038800 A CN201210038800 A CN 201210038800A CN 102557766 B CN102557766 B CN 102557766B
- Authority
- CN
- China
- Prior art keywords
- phosphorus
- paenibacillus macerans
- soil
- zju0902
- sources
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses an application of a Paenibacillus macerans strain in the dissolution of phosphorus sources in soil or eutrophic water. The Paenibacillus macerans strain is Paenibacillus macerans ZJU0902 with a preservation number of CCTCC NO:M209158; the phosphorus source is at least one of inorganic phosphorus sources and organic phosphorus sources. According to the invention, Paenibacillus macerans ZJU0902 is used for the dissolution of inorganic and organic phosphorus sources in soil, and has the characteristics of stability, high efficiency, and wide spectra for phosphorus dissolution; After a microbial fertilizer prepared by mixing the bacterial strain fermentation broth with a fertilizer matrix is applied to soil, dominant microflora are formed rapidly; the fertilizer efficiency is good; the effective phosphorus content of the soil can be improved significantly; and the healthy growth of plants is promoted; the bacterial strain is used for the dissolution of inorganic and organic phosphorus sources in eutrophic water, can promote the absorption of superfluous phosphorus element in water by water plants, and thus prevent and control water eutrophication.
Description
Technical field
The present invention relates to microbial technology field, relate in particular to the application in a kind of Paenibacillus macerans strain phosphorus source in dissolving soil or eutrophication water.
Background technology
Plant-growth needs some essential nutritive elements, and phosphorus is a kind of principal element that can affect plant health growth wherein.Phosphorus is not only the component of many important compound in plant materials, but also with the important metabolism in number of ways involved in plant body.But in soil, 90% phosphorus all exists with the indissoluble state, this may be due to the easy and Fe under acidic conditions of the phosphorus in natural soils
3+, Al
3+the plasma complexing, form FePO
4and AlPO
4deng difficultly-soluble phosphates; Under alkaline condition again with Ca
2+ion complexation, form Ca
3(PO
4)
2or CaHPO
4etc. inertia phosphoric acid salt, these phosphorus all can not directly be absorbed by plant, thereby hinder the healthy growth of plant, and make output can not get maximizing.
Body eutrophication refers to the water pollution phenomenon that the plant nutrient substance content such as nitrogen, phosphorus too much cause, result causes aquatic ecosystem to lose the ability of self―sustaining, self-control, causes the further aggravation of environmental pollution.According to statistics, China's eutrophication and supereutrophic lake reach respectively 66% and 22% (silk floss waits well .2010 recklessly) of lake total amount.Improving waterplant the receptivity of the inorganic elementss such as nitrogen, phosphorus is prevented and treated to body eutrophication, is an important subject of current field of Environment Protection.
Existing some microorganisms in soil can turn to the phosphate transfection of insoluble can utilize form, and these microorganisms are called phosphate solubilizing microorganism (phosphate-solubilizing microorganisms, PSM).Phosphate solubilizing microorganism can rely on the meta-bolites of self or dissolve indissoluble inorganic phosphorus or the organophosphorus in soil with other biological synergetic, thereby plays an important role in the phosphorus element transforms.Therefore the research of phosphate solubilizing microorganism is subject to scientist's attention always, and expectation improves the validity of soil insoluble phosphorus and the utilising efficiency of phosphate fertilizer with the approach of Inoculant.Due to the phosphorus decomposing ability between different types of phosphate solubilizing bacteria or bacterial strain and effect of inoculation difference great disparity, so that the screening of efficient phosphate-solubilizing bacterial strain seems is particularly important.Although the phosphorus decomposing ability of fungi is greater than bacterium, in microbial fertilizer is produced, the phosphate-solubilizing bacteria proportion is far above fungi.In microbial fertilizer production, separate at present phosphorus stabilizer, bacterial strain efficient, wide spectrum is also fewer, this has proposed urgent task (Fan BQ, 2002) for the seed selection work of strain excellent.
Paenibacillus macerans is split into (Guemori-Athmani et al.2000 from the rhizosphere of a lot of plants; Von der Weid et al.2000).Research shows, soaks crudefiber crop gemma bar and belongs to that bacterium is heat-resisting, stable, natural adaptation power is strong, is not only at present growth-promoting bacterium (the Artursson et al.2006 of plant-growth; Or biocontrol microorganisms (the Timmusk et al.2009 of plant opposing multiple diseases Algam et al.2010); Li et al.2007,2010,2011a, b).Veritably, Paenibacillus macerans has been found to be involved in plant growth-promoting (Timmusk et al.1999) and germ inhibition (Bae et al.2000; Timmusk 2003; Li et al.2011a, b), but also there is no about Paenibacillus macerans at present the report as the phosphorus dissolution of bacteria.Therefore, the phosphorus dissolving power of research Paenibacillus macerans is for clearly this schizomycete growth-promoting and diseases prevention mechanism have very important significance.
Summary of the invention
The invention provides the application in a kind of Paenibacillus macerans strain phosphorus source in dissolving soil or eutrophication water, this Paenibacillus macerans strain can stablize, efficiently, the inorganic or organic phosphorus sources in wide spectrum ground dissolving soil or eutrophication water.
The application in a kind of Paenibacillus macerans strain phosphorus source in dissolving soil or eutrophication water, described Paenibacillus macerans strain is Paenibacillus macerans (Paenibacillus macerans) ZJU0902, and preserving number is CCTCC NO:M 209158; Described phosphorus source is at least one in inorganic phosphorous sources and organic phosphorus sources.
Described Paenibacillus macerans ZJU0902 preservation, be preserved in the Chinese Typical Representative culture collection center (CCTCC) that is positioned at Wuhan City Luo Jiashan Wuhan University, and preservation date is on July 19th, 2009, and deposit number is CCTCC NO:M 209158.This bacterial strain is disclosed in the application for a patent for invention that publication number is CN101712942A, and its screening, cultural method, form and biological property etc. are disclosed in detail, this bacterial strain is to separate and obtain in topsoil around the cucumber rhizosphere in the serious cucumber ground occurred of one, Hangzhou, Zhejiang province city bacterial wilt, through being accredited as Paenibacillus macerans, plant-bacterial-wilt is had to certain prevention effect.
Described soil can be Ca
3(PO
4)
2, CaHPO
4, FePO
4or AlPO
4higher Deng insoluble phosphate content, and need the weak soil of phosphorus plant growing way.
Describedly need the phosphorus plant can be the plant that crop in cruciferae, leguminous crop, tomato, eggplant or beet etc. are very responsive to phosphorus, or also can need for corn, sesame etc. are medium the phosphorus plant.
Described eutrophication water can be for being rich in the eutrophication water of phosphoric.
Described inorganic phosphorous sources can be Ca
3(PO
4)
2, CaHPO
4, FePO
4and AlPO
4in one or more mixtures.
Preferably, described inorganic phosphorous sources is Ca
3(PO
4)
2or CaHPO
4.
Described organic phosphorus sources can be phytic acid.
Described application can be as follows: activated Paenibacillus macerans ZJU0902 is inoculated in the LB liquid nutrient medium, cultivates 12-24h in 28-30 ℃, make seed liquor; Seed liquor is added in soil or eutrophication water, mix;
Wherein, in every kilogram of soil or every liter of eutrophication water, the add-on of described seed liquor is 5-10mL.
For the convenient phosphorus decomposing ability of analyzing bacterial strain, can Design Laboratory phosphorus decomposing experimental study bacterial strain to the dissolving of P source compound.
Described laboratory phosphorus decomposing test can be as follows: Paenibacillus macerans ZJU0902 is inoculated in the NBRIP liquid nutrient medium that is added with the phosphorus source, in 28 ℃ of shaking tables, cultivates 1-7d;
Wherein, in volume 1L, described NBRIP liquid nutrient medium comprises following component: glucose 10g, and magnesium chloride hexahydrate 5g, magnesium sulfate heptahydrate 0.25g, Repone K 0.2g, ammonium sulfate 0.1g, surplus is water, pH 7.0; The addition in described phosphorus source is counted 0.001-0.04mol with every liter of NBRIP liquid nutrient medium.
Described NBRIP liquid nutrient medium is substratum commonly used in microorganism phosphorus decomposing research, and it is sensitiveer that this substratum detects phosphorus content.
Described shaking table incubation time is preferably 4-6d; 5d more preferably, the phosphorus decomposing effect is best.
The present invention also provides a kind of microbial fertilizer that can dissolve the soil phosphorus source, and described microbial fertilizer contains following composition: cell concentration is greater than 10
8the full phosphorus that the organic matter that the full nitrogen that the Paenibacillus macerans strain of individual/g, mass concentration are 4-6%, mass concentration are 35-40% and mass concentration are 1-2%;
Wherein, described Paenibacillus macerans strain is Paenibacillus macerans ZJU0902, and preserving number is CCTCC NO:M 209158;
Contain the organonitrogen that mass concentration is 90%-100% in described full nitrogen.
Described microbial fertilizer can impose in the soil of plantation crop in cruciferae, leguminous crop, tomato, eggplant, beet, corn or sesame.
The present invention also provides the preparation method of mentioned microorganism fertilizer, comprising: Paenibacillus macerans ZJU0902 is inoculated in the LB enriched medium, in 28-30 ℃ of cultivation 24-48h, obtains fermented liquid; Fermented liquid is added in base-material by weight the ratio of 4-6%, mix, in 20-28 ℃ of fermentation 3-5d, make microbial fertilizer;
Wherein, described LB enriched medium adds ammonium nitrate, sulphur ammonium and analysis for soybean powder and makes in the LB liquid nutrient medium;
Described base-material is one or more mixtures in feces of livestock and poultry, crop material, inorganic nitrogenous fertilizer and bean dregs.
Preferably, described microbial fertilizer also can be mixed by Paenibacillus macerans ZJU0902 fermented liquid and organic fertilizer with high nitrogen.The preparation of described organic fertilizer with high nitrogen can be with reference to the relevant record in the patent of invention of publication number CN1215022C.
In the present invention, described Paenibacillus macerans ZJU0902 separates and obtains from plant rhizosphere soil, and it can adapt to complicated edatope; Simultaneously, laboratory phosphorus decomposing test and plant test-results show, this bacterial strain has dissolving power to multiple inorganic or organic phosphorus sources, and hence one can see that, and this bacterial strain can be used for the dissolving in Soil Phosphorus source.On the other hand, according to the laboratory phosphorus decomposing, test, Paenibacillus macerans ZJU0902 is good to phosphorus source solute effect in the NBRIP liquid nutrient medium, shows that this Paenibacillus macerans ZJU0902 can be for dissolving the phosphoric of liquid environment, as the dissolving in phosphorus source in eutrophication water.
To separate in soil, the Paenibacillus macerans ZJU0902 obtain is inorganic for soil, the dissolving of organic phosphorus sources in the present invention, has the advantages that to separate phosphorus stabilizer, efficient, wide spectrum, and is conducive to plant and absorbs the essential phosphoric of growth, promotes the growing way of plant; Mix with fertilizer matrix the microbial fertilizer made with this bacterial strain fermentation liquor, after being manured into soil, can form rapidly dominant microflora, fertilizer efficiency is good, can make available phosphorus content in soil significantly improve, and promotes the healthy growth of plant.This bacterial strain is inorganic for eutrophication water, the dissolving of organic phosphorus sources, can impel waterplant to absorb unnecessary phosphoric in water body, thus the control body eutrophication.
The accompanying drawing explanation
Fig. 1 is the standard phosphorus curve of setting up with 50mg/l phosphoric acid standardized solution in embodiment 1;
Fig. 2 is that in embodiment 3, Paenibacillus macerans ZJU0902 dissolves the size of loop diameter to different phosphate sources;
Fig. 3 is the impact of inoculation time on Paenibacillus macerans ZJU0902 phosphorus dissolving power in embodiment 5; Wherein, abcd means the significant difference between different treatment;
Fig. 4 is the impact of inoculation time on Paenibacillus macerans ZJU0902 thalli growth in embodiment 5; Wherein, abcdef means the significant difference between different treatment.
Embodiment
In following examples, Paenibacillus macerans used (Paenibacillus macerans) ZJU0902 (is preserved in Chinese Typical Representative culture collection center, deposit number is CCTCC M209158, the preservation time is on July 19th, 2009) the application for a patent for invention that has been all CN101712942A at publication number such as screening, cultural method, form and biological property in open, be specially:
This bacterial strain be from the serious cucumber ground occurred of one, Hangzhou, Zhejiang province city bacterial wilt around the cucumber rhizosphere in topsoil separation screening obtain, be accredited as Paenibacillus macerans (Paenibacillus macerans) through Biolog, lipid acid and 16S rDNA.Biological characteristics is as follows: cell is shaft-like, and Gram-positive is moved with peritrichous.Oval gemma is arranged expanding in packing, on nutrient agar medium without soluble pigment.Amphimicrobian.Bacterium colony on substratum is thin, is shape expansion, and the bacterium amount is many and stick mattness.Grower can be adhered on substratum.Growth optimum temperuture 28-30 ℃, growth temperature range 15-50 ℃.The growth optimal pH is 7.0, and growth pH scope is 5.6-8.5.
By KH
2pO
4dry 4h under 105 ℃, accurately take 0.2195g KH
2pO
4soluble in water and move on in the volumetric flask of 1000ml, add 400ml water and the 5ml vitriol oil, constant volume, to 1000ml, obtains 50mg/l phosphoric acid standardized solution.As the phosphorus source, be made into the standardized solution of series concentration with 50mg/l phosphoric acid standardized solution, set up one about OD
450the typical curve of phosphorus content in value and solution, the OD that can survey in experiment by typical curve
450value obtains corresponding phosphorus content.
Wherein, the mensuration of phosphorus content adopts the yellow light-intensity method of phosphorus molybdenum, is specially:
(1) take 2.4g NaOH, add water and constantly stir, constant volume is made into 6N NaOH solution to 100ml;
Take 0.25g 2,4-dinitrophenol solid, add the 12.5ml dehydrated alcohol, and then constant volume, to 100ml, is made into 2,4-dinitrophenol indicator;
Take 0.5g (NH
4)
6moO
24h
2o is dissolved in 200ml distilled water, takes 0.625gNH
4vo
3in 150ml boiling water, wait after cooling and add the 125ml concentrated nitric acid, drop to room temperature; At leisure ammonium molybdate solution is poured in ammonium metavanadate solution, constantly stirred, last constant volume, to 500ml, is made into ammonium meta-vanadate and ammonium molybdate mixed solution.
(2) after the phosphoric acid standardized solution of series concentration is centrifugal, pipette the 10ml supernatant liquor in the 25ml volumetric flask, splash into 12,4-dinitrophenol indicator, then dropwise add 6N NaOH to make solution yellow just occur, add ammonium meta-vanadate and ammonium molybdate mixed solution 5ml, constant volume, to 25ml, is at room temperature placed 15min, surveys OD
450value.
With three zoning collimation methods, the Paenibacillus macerans ZJU0902 of preservation is rule on the NA agar plate, be placed under 28 ℃ and cultivate 24h; The mono-bacterium colony of Paenibacillus macerans ZJU0902 is received in the LB liquid nutrient medium, and in 28 ℃, in the shaking table of 160r/min, shaking culture 12h, make seed liquor, and in seed liquor, cell concentration is 10
8cFU/ml.
The mensuration of phosphorus dissolving power in embodiment 3 solid mediums
Add respectively 5g/l inorganic phosphorus Ca in NBRIP liquid nutrient medium (glucose 10g, magnesium chloride hexahydrate 5g, magnesium sulfate heptahydrate 0.25g, Repone K 0.2g, ammonium sulfate 0.1g, distilled water 1000ml, pH7.0)
3(PO
4)
2, CaHPO
4, FePO
4and AlPO
4and 5ml/l organophosphorus phytic acid is as the phosphorus source, then add agar (20g/l) to be mixed with solid medium.A kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices after autoclaving, pick Paenibacillus macerans ZJU0902 that purifying in embodiment 2 cultivates to flat board with sterilized toothpick, 30 ℃ of unglazed cultivations.Detect the diameter that transparent phosphorus dissolves circle after 4 weeks.Repeat 4 times.
The result demonstration, Paenibacillus macerans ZJU0902 can dissolve Ca
3(PO
4)
2, CaHPO
4with the phosphoric in phytic acid, and to the phosphorus meltage maximum of phytic acid.In solid medium, Paenibacillus macerans ZJU0902 can not dissolve FePO
4, AlPO
4in phosphoric (Fig. 2).
The mensuration of phosphorus dissolving power in embodiment 4 liquid nutrient mediums
Pour 20ml NBRIP liquid nutrient medium (glucose 10g, magnesium chloride hexahydrate 5g, magnesium sulfate heptahydrate 0.25g, Repone K 0.2g, ammonium sulfate 0.1g, distilled water 1000ml, pH7.0) in the 50ml Erlenmeyer flask, add respectively 5g/l inorganic phosphorus Ca
3(PO
4)
2, CaHPO
4, FePO
4and AlPO
4and 5ml/l organophosphorus phytic acid is as the phosphorus source.It is 10 that Paenibacillus macerans ZJU0902 seed liquor in inoculation embodiment 2 makes the bacterium starting point concentration
7cFU/ml cultivates 3d on 28 ℃, 130r/min shaking table, collects the centrifugal 10min of 10000r/min after bacteria suspension, adopts the OD of the yellow spectrphotometric method for measuring supernatant liquor of phosphorus molybdenum in embodiment 1
450the value, according to the standard phosphorus curve in embodiment 1 by OD
450value converts phosphorus content to.
The result demonstration, Paenibacillus macerans ZJU0902 can dissolve different inorganic phosphorus and organophosphorus, and to Ca
3(PO
4)
2, CaHPO
4, phytic acid the phosphorus dissolving power very strong.Though compare with contrasting separately (not inoculating strain, other is identical), the phosphorus meltage all significantly increases, Paenibacillus macerans ZJU0902 is to Ca
3(PO
4)
2, CaHPO
4the phosphorus meltage increase 3-12 doubly, to FePO
4the phosphorus meltage increase more than 1 times, and to AlPO
4, phytic acid the phosphorus meltage increase minimum.This explanation bacterium is different to the phosphorus dissolving power of different phosphate sources, the most applicable dissolving of Paenibacillus macerans ZJU0902 Ca
3(PO
4)
2, CaHPO
4(in Table 1).
The phosphorus dissolving power of table 1 Paenibacillus macerans ZJU0902 to different phosphate sources
Annotate: ab means the significant difference between different treatment.
The impact of embodiment 5 inoculation times on Paenibacillus macerans ZJU0902 phosphorus dissolving power
Pour 20ml NBRIP liquid nutrient medium (glucose 10g, magnesium chloride hexahydrate 5g, magnesium sulfate heptahydrate 0.25g, Repone K 0.2g, ammonium sulfate 0.1g, distilled water 1000ml, pH7.0) in the 50ml Erlenmeyer flask, add 5g/l Ca
3(PO
4)
2as the phosphorus source.It is 10 that Paenibacillus macerans ZJU0902 seed liquor in inoculation embodiment 2 makes the bacterium starting point concentration
7cFU/ml cultivates 1d on 28 ℃, 130r/min shaking table, 2d, and 3d, 4d, 5d, 6d, 7d, collect respectively the centrifugal 10min of 10000r/min after bacteria suspension, adopts the OD of the yellow spectrphotometric method for measuring supernatant liquor of phosphorus molybdenum in embodiment 1
450the value, according to the standard phosphorus curve in embodiment 1 by OD
450value converts phosphorus content to.Measure the Paenibacillus macerans ZJU0902 cell quantity of corresponding time with colony counting method.
As shown in Figure 3 and Figure 4, the phosphorus content after inoculation 2d in solution reaches 21.8 μ g/ml to result, and with inoculation, 1d does not have significant difference; Inoculation 2-3d during this period of time in, the maximum of advancing the speed of the phosphorus content in solution, the phosphorus content after inoculation 3d in solution reaches 54.9 μ g/ml, is 2.5 times of inoculation 2d phosphorus content.Inoculation 3-5d interior phosphorus content during this period of time slowly increases, and the phosphorus content after inoculation 5d in solution reaches maximum value 58.6 μ g/ml.Inoculation 5-7d, phosphorus content slightly descends, but there is no significant difference.Therefore inoculate 5d Paenibacillus macerans ZJU0902 the dissolving of calcium phosphate is reached to maximum value, 5d is best inoculation time.It is inconsistent that the phosphorus content of this and Son et al. (2006) increases with inoculation time the report descended, and may cause due to phosphorus dissolution of bacteria kind difference.
In addition, the thalli growth result shows, the thalline quantity after inoculation 2d in solution reaches maximum value, than the initial 2.4log10 CFU/ml that increased, this well explain after the inoculation 2-3d during this period of time in the phosphorus content maximum phenomenon of advancing the speed.
Along with the increase of inoculation time, thalline quantity starts to reduce, and to inoculation 7d cell quantity, reaches 7.66 log10 CFU/ml.
Preparation NBRIP liquid nutrient medium (glucose 10g, magnesium chloride hexahydrate 5g, magnesium sulfate heptahydrate 0.25g, Repone K 0.2g, ammonium sulfate 0.1g, distilled water 1000ml, pH 7.0), add 5g/l Ca
3(PO
4)
2as the phosphorus source.Draw the 10ml nutrient solution in the culture dish that is covered with 3 layers of sterilizing filter paper.Soak 4h in Paenibacillus macerans ZJU0902 bacteria suspension prepared at embodiment 2 by cucumber seeds or sterile distilled water, transfer in culture dish, in 16 hours 21 ℃/8 hours 19 ℃ of artificial culture casees, dark vernalization is 3 days, measures percentage of germination.After seed germination, supplement the 5ml nutrient solution every day, to guarantee the seed seedling, enough moisture arranged and can make its root therefrom absorb phosphoric.Gather in the crops cucumber seedling after 10 days and measure root, stem fresh weight.Each processes 5 repetitions, and each repeats 80 seeds.
Result shows, the Seed Germination in Cucumber rate of processing with Paenibacillus macerans ZJU0902 bacteria suspension reaches 87%, the Seed Germination in Cucumber rate of processing with distilled water reaches 85%, this explanation Paenibacillus macerans ZJU0902 can not affect the normal germination of cucumber seeds, and the percentage of germination of cucumber seeds itself is just very high.In addition, from root, the stem fresh weight of cucumber seedling, can see, the cucumber seedling growing way of processing with Paenibacillus macerans ZJU0902 bacteria suspension is better than contrast.This explanation Paenibacillus macerans ZJU0902 is by the Ca in nutrient solution
3(PO
4)
2be degraded into available phosphorus, promote the absorption of cucumber seedling to phosphoric.
The table 2 Paenibacillus macerans ZJU0902 Ca that degrades
3(PO
4)
2impact on plant germination and growth of seedling
Annotate: ab means the significant difference between different treatment.
The production of embodiment 7 phosphate-solubilizing bacteria Paenibacillus macerans ZJU0902 microbial organic fertilizers
(1) preparation of Paenibacillus macerans ZJU0902 fermented liquid: the seed liquor in embodiment 2 is pressed to inoculum size 5-10% (v/v), rotating speed 130-160r/min, culture temperature 28-30 ℃, incubation time 24-48h, at fermention medium, (the LB substratum adds ammonium nitrate, sulphur ammonium, analysis for soybean powder etc., pH 6.8-7.2) fermentation culture in, obtain high density fermentation liquid (bacteria containing amount>10
10individual/ml).
(2) preparation of organic carrier: by Chinese patent " method and the fertilizer product thereof that utilize agricultural solid residue to make ", the disclosed method of the patent No.: ZL200410014773.1 is produced organic fertilizer with high nitrogen, with the organic carrier of this as fertilizer sources.
(3) preparation of microbial fertilizer: fermented liquid is added in organic carrier by weight 5% ratio, after fully mixing, place under field conditions (factors) 3-5d, moisture to the water content that makes to scatter and disappear is less than 30%, obtains the phosphate solubilizing microorganism organic fertilizer.
After measured, in the finished product, bacterial number reaches 10
8individual/more than g, total nitrogen content reaches 4%, and wherein organonitrogen content reaches more than 90%, and organic content reaches 35%, and content of tatal phosphorus is 1-2%.Product index reaches the standard of national microorganism organic fertilizer.
Crop in cruciferae, leguminous crop, tomato, eggplant or beet etc. are the crops very responsive to phosphorus.Wherein rape, tomato Chang Zuowei lack phosphorus indication crop for judging whether field has sufficient available phosphorus.
In Hangzhou, the ground such as Jinhua chooses the high and field that available phosphorus content is low of phosphorus total content, the microorganism organic fertilizer that imposes on the present embodiment is executed, maize planting, cucumber plant, and contrast is set.Observe whole growth course, lack phosphorus milpa slight of stature, cauline leaf is obvious red-purple mostly, and filigree is extracted out late, female fringe deformity, and fringe is little, and setting percentage is low, postpones ripe.Cucumber lacks short and small deadlock of the whole strain of phosphorus, and dark green, Lao Ye occurs that sorrel is shrivelled, the cucumber color of tying yellow and little.And that the microorganism organic fertilizer of executing the present embodiment is executed corn and the cucumber plant growing way of planting on block is good, cauline leaf is luxuriant, grow normal, tie corn and cucumber yield obviously increases.
Claims (5)
1. the application in Paenibacillus macerans strain phosphorus source in dissolving soil or eutrophication water, it is characterized in that, described Paenibacillus macerans strain is Paenibacillus macerans (Paenibacillus macerans) ZJU0902, and preserving number is CCTCC NO:M209158; Described phosphorus source is at least one in inorganic phosphorous sources and organic phosphorus sources.
2. application according to claim 1, is characterized in that, described inorganic phosphorous sources is Ca
3(PO
4)
2, CaHPO
4, FePO
4and AlPO
4in one or more mixtures.
3. application according to claim 2, is characterized in that, described inorganic phosphorous sources is Ca
3(PO
4)
2or CaHPO
4.
4. application according to claim 1, is characterized in that, described organic phosphorus sources is phytic acid.
5. application according to claim 1, is characterized in that, comprises the steps: activated Paenibacillus macerans ZJU0902 is inoculated in the LB liquid nutrient medium, in 28-30 ℃ of cultivation 12-24h, makes seed liquor; Seed liquor is added in soil or eutrophication water, mix;
Wherein, in every kilogram of soil or every liter of eutrophication water, the add-on of described seed liquor is 5-10mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100388003A CN102557766B (en) | 2012-02-21 | 2012-02-21 | Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100388003A CN102557766B (en) | 2012-02-21 | 2012-02-21 | Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102557766A CN102557766A (en) | 2012-07-11 |
| CN102557766B true CN102557766B (en) | 2013-12-11 |
Family
ID=46404550
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100388003A Expired - Fee Related CN102557766B (en) | 2012-02-21 | 2012-02-21 | Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102557766B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104293693A (en) * | 2014-07-14 | 2015-01-21 | 东华大学 | Method for preparing jute fiber by utilizing aneurinibacillus migulanus DY3 strain through retting |
| CN104277995B (en) * | 2014-07-14 | 2017-01-11 | 东华大学 | Aneurinibacillus migulanus DY3 strain and application thereof |
| CN105985917B (en) * | 2015-02-06 | 2019-12-13 | 中国农业大学 | Method for increasing biomass of chlorella in pig-raising wastewater |
| CN106754371A (en) * | 2016-12-06 | 2017-05-31 | 广州富生源环保工程有限公司 | A kind of microbial bacterial agent preparation method and application for improving soil phosphorus utilization |
| CN108504586B (en) * | 2017-12-06 | 2020-05-05 | 四川明湖环保科技有限公司 | Paenibacillus softening and straw decomposing agent |
| CN110643554B (en) * | 2019-11-26 | 2020-03-06 | 中国科学院烟台海岸带研究所 | Strain capable of efficiently removing inorganic phosphorus in water body and application thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067040A (en) * | 1992-07-09 | 1992-12-16 | 清华大学 | The preparation technology of bioactive P-K composite fertilizer |
| CN1158328A (en) * | 1996-10-25 | 1997-09-03 | 李�杰 | Organic and inorganic mixed fertilizer and its prepn. method |
| CN1569764A (en) * | 2004-04-28 | 2005-01-26 | 南京农业大学 | Method for making organic high nitrogen fertilizer using agricultural solid waste and fertilizer products produced thereby |
| WO2009086811A2 (en) * | 2008-01-10 | 2009-07-16 | Schmack Biogas Ag | Paenibacillus macerans for the generation of biogas |
| CN101538173A (en) * | 2009-05-08 | 2009-09-23 | 北京化工大学 | Method for producing microbial compound bacterial fertilizer |
| CN101712942A (en) * | 2009-11-30 | 2010-05-26 | 浙江大学 | Paenibacillus macerans for preventing and controlling plant bacterial wilt and application thereof |
| WO2010072220A1 (en) * | 2008-12-23 | 2010-07-01 | Schmack Biogas Ag | Paenibacillus macerans for the treatment of biomass |
| CN102154156A (en) * | 2010-12-28 | 2011-08-17 | 南京农业大学 | Antagonists capable of preventing and controlling tomato bacterial wilt or knot nematode and microbial organic fertilizer thereof |
-
2012
- 2012-02-21 CN CN2012100388003A patent/CN102557766B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067040A (en) * | 1992-07-09 | 1992-12-16 | 清华大学 | The preparation technology of bioactive P-K composite fertilizer |
| CN1158328A (en) * | 1996-10-25 | 1997-09-03 | 李�杰 | Organic and inorganic mixed fertilizer and its prepn. method |
| CN1569764A (en) * | 2004-04-28 | 2005-01-26 | 南京农业大学 | Method for making organic high nitrogen fertilizer using agricultural solid waste and fertilizer products produced thereby |
| WO2009086811A2 (en) * | 2008-01-10 | 2009-07-16 | Schmack Biogas Ag | Paenibacillus macerans for the generation of biogas |
| WO2010072220A1 (en) * | 2008-12-23 | 2010-07-01 | Schmack Biogas Ag | Paenibacillus macerans for the treatment of biomass |
| CN101538173A (en) * | 2009-05-08 | 2009-09-23 | 北京化工大学 | Method for producing microbial compound bacterial fertilizer |
| CN101712942A (en) * | 2009-11-30 | 2010-05-26 | 浙江大学 | Paenibacillus macerans for preventing and controlling plant bacterial wilt and application thereof |
| CN102154156A (en) * | 2010-12-28 | 2011-08-17 | 南京农业大学 | Antagonists capable of preventing and controlling tomato bacterial wilt or knot nematode and microbial organic fertilizer thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102557766A (en) | 2012-07-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103789223B (en) | Acinetobacter calcoaceticus with free living nitrogen fixation and phosphorus and potassium dissolving capability and application of acinetobacter calcoaceticus | |
| CN100545259C (en) | A kind of lead and cadmium tolerant bacteria and be used for the plant restoration method of heavy metal pollution of soil | |
| CN101643704B (en) | Phosphorus dissolvable penicillium oxalicum P8 | |
| CN102557766B (en) | Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water | |
| CN102344812B (en) | Microbiological preparation for improving alkaline land, its preparation method and its application | |
| CN110305671B (en) | Soil biological improver for continuous cropping of greenhouse vegetables and preparation and use methods thereof | |
| CN103539535B (en) | Active biological matrix product specially used for culture of cucumber seedlings | |
| CN104974962B (en) | One plant of acinetobacter phosphorus decomposing growth-promoting bacterium Y40 and its application | |
| CN102432393A (en) | Composite organic soil for raising rice seedlings | |
| CN110184220A (en) | One plant height imitates dissolving phosphor and dissolving potassium bacterium and its application | |
| CN111484951B (en) | Bacillus for dissolving phosphorus and fixing nitrogen and application thereof in growth promotion | |
| CN106748114A (en) | A kind of rice in saline-alkali field special fertilizer | |
| CN106397032A (en) | Special fertilizer for saline land peanut | |
| CN107652147A (en) | Improved soil type fertilizer synergistic conditioning agent and its production method | |
| CN106754463A (en) | One plant of tool dissolving P capacity Burkholderia bacterium NJAU B8 and its microbial manure of development | |
| CN104447054A (en) | Special biological organic fertilizer for tea | |
| CN108610181A (en) | A kind of vegetables special bio bacterial manure and the preparation method and application thereof | |
| CN105646015A (en) | Composite microelement fertilizer containing chlorella and paenibacillus, preparation and applications thereof | |
| CN102503653A (en) | Organic compound soil for rice seedling raising | |
| CN104974954A (en) | Application of grass phosphorus-dissolving plant growth promoting rhizobacterium | |
| CN105112319A (en) | Pseudomonas phosphate dissolving bacterium Y2, bioorganic fertilizer prepared through using bacterium, and application of fertilizer | |
| CN101812410B (en) | Streptomycesvinaceus-drappus microbial inoculum and method for preparing same | |
| CN109874443A (en) | A kind of method of cadmium pollution dryland soil long-term cropping | |
| CN104817372A (en) | Special biological phosphorus fertilizer for cucumber, and preparation method thereof | |
| CN106431625A (en) | Special fertilizer for rice in saline and alkaline land |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131211 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |