CN104277995A - Aneurinibacillus migulanus DY3 strain and application thereof - Google Patents
Aneurinibacillus migulanus DY3 strain and application thereof Download PDFInfo
- Publication number
- CN104277995A CN104277995A CN201410333874.9A CN201410333874A CN104277995A CN 104277995 A CN104277995 A CN 104277995A CN 201410333874 A CN201410333874 A CN 201410333874A CN 104277995 A CN104277995 A CN 104277995A
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- strain
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- bacterial strain
- jute
- fibre
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
- D01C1/00—Treatment of vegetable material
- D01C1/04—Bacteriological retting
Abstract
The invention relates to an Aneurinibacillus migulanus DY3 strain and application thereof. The 16Sr DNA (deoxyribonucleic acid) of the Aneurinibacillus migulanus DY3 strain is disclosed as SEQ ID NO:1. The strain is used for preparing ambary fiber or jute fiber by retting. The Aneurinibacillus migulanus DY3 strain has the advantages of coarse culture conditions, favorable heat resistance, high growth and reproduction speed, stable enzyme activity and non-toxic bacterium and enzyme, can effectively remove non-cellulose colloids in the ambary or jute fiber, and has favorable application prospects.
Description
Technical field
The invention belongs to retted fibre field, particularly a kind of Michaelis VitB1 genus bacillus DY3 bacterial strain and application thereof.
Background technology
Fiber crops are one of textile raw materials, and its kind mainly contains flax, ramie, hemp, bluish dogbane, jute, kendir, sisal hemp, abaca, nettle and piemarker etc.Flaxen fiber is the general designation of the fiber obtained from various numb plant, has the advantage that moisture absorption, ventilative, heat radiation and other natural fibers such as antibacterial are incomparable.China is as one of the abundantest country of hemp resource in the world, and the annual flaxen fiber produced and goods thereof, except the demand meeting domestic market, also as the export of commodities in large amounts U.S., West Europe and country in Southeast Asia, earn foreign exchange nearly 10,000,000,000 dollars every year.Reduce discharging policy and human consumer's back to nature at national energy-saving, pursue under green consumption idea promotes, bast-fibre green processing technologies has become the study hotspot of field of textiles.
Retted fibre utilizes bacterium and moisture to the effect of plant, dissolves or corrodes the cellular reticular tissue of major part and colloid that are enclosed in outside bast fibre bundle, thus impelling the course of processing that fiber is separated with straw.In the bubble method of widespread use, straw is immersed in water, and water permeation, to the middle body of straw, makes inner cell expansion, bursts outermost layer, thus increases the absorption to moisture and bacterium.Process of retting flax with is the complete processing of producing good fiber quality, and the bacterial classification selection of retted fibre process is the key of retted fibre.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Michaelis VitB1 genus bacillus DY3 bacterial strain and application thereof, this strain culturing condition is extensive, good heat resistance, growth and breeding is fast, effectively can remove the non-cellulose colloid in bluish dogbane or jute fibre, and enzyme is lived stable, bacterium, the equal nontoxicity of enzyme, have a good application prospect.
A kind of Michaelis VitB1 genus bacillus DY3 bacterial strain of the present invention, its 16Sr DNA sequence dna is as shown in SEQ ID NO:1.
The application of a kind of Michaelis VitB1 genus bacillus DY3 bacterial strain of the present invention, described bacterial strain is applied to retted fibre and prepares bastose or jute fibre.
Described bastose or jute fibre are applied to and make gunnysack, burlap, papermaking, rope, weavening carpet or curtain.
The preserving number of Michaelis VitB1 genus bacillus (Aneurinibacillus migulanus) DY3 bacterial strain of the present invention is CGMCC No.9121, preservation day is on May 4th, 2014, suggestion Classification And Nomenclature is: Michaelis VitB1 genus bacillus (Aneurinibacillus migulanus), depositary institution's title: CGMCC China Committee for Culture Collection of Microorganisms common micro-organisms center, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica.
According to 16sR DNA sequencing Blast and cluster analysis result, this bacterium and Michaelis VitB1 genus bacillus similarity are 99%, in conjunction with microscopy analysis, this bacterial strain is named to be Michaelis VitB1 genus bacillus (Aneurinibacillus migulanus) DY3 bacterial strain.During 16S rDNA checks order, the forward primer 27F adopted, reverse primer 1492R, be international primer, synthesized by Shanghai Sangon Biological Engineering Technology And Service Co., Ltd.
beneficial effect
Culture condition of the present invention is extensive, good heat resistance, and growth and breeding is fast, effectively can remove the non-cellulose colloid in bluish dogbane or jute fibre, and enzyme is lived stable, and bacterium, the equal nontoxicity of enzyme, have a good application prospect.
Accompanying drawing explanation
Fig. 1 is bacterial strain uses therefor microscope stained photographs (oily mirror) of the present invention;
Fig. 2 a-j is the DNA of bacteria collection of illustrative plates that the present invention adopts primer 2 7F to produce;
Fig. 3 a-k is the DNA of bacteria collection of illustrative plates that the present invention adopts primer 1492R to produce.
Embodiment
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after the content of having read the present invention's instruction.
Embodiment 1
The retted fibre time determines according to bluish dogbane or jute harvest time, is generally mid or late September to mid or late October.
Retting flax method:
(1) the Michaelis VitB1 genus bacillus DY3 bacterial strain preserved is cultivated in beef-protein medium (every 1000ml substratum comprises extractum carnis 3g, peptone 10g, sodium-chlor 5g and water 1000ml), add frozen damping fluid (the frozen damping fluid of every 100ml comprises glycerine 50ml and water 50ml), culture condition is 37 DEG C, pH nature, incubation time 12 hours;
(2) in bluish dogbane or jute bran mass (every 1000ml substratum comprises bluish dogbane or jute skin 200g, wheat bran 50g, wort 50ml and water 950ml) 1000ml, bacterium one ring is accessed, shake-flask culture, culture condition is 37 DEG C, pH nature, incubation time 24 hours;
(3) (every 1000ml substratum comprises bluish dogbane or jute skin 100g culture medium inoculated to be entered bluish dogbane or jute wheat bran fermention medium, wheat bran 100g, wort 200ml and water 800ml), shake-flask culture obtains zymocyte liquid, culture condition is 37 DEG C, pH nature, incubation time 24 hours;
(4) preparation in retted fibre pond: with 4HB-800 type bluish dogbane or jute peeling machine fresh hide is separated from bluish dogbane or the full bar of jute (phloem because of mechanical presses, re-pack impact caused many wounds, be beneficial to degumming bacterium invasion, but do not destroy fiber); Afterbody end is neat (can be got rid of as degree during to wash fiber crops, about 20 is one), ending is held together together, straight, fold becomes three to four layers, and (retted fibre tank depth is similar, numb is just in time flooded during immersion) or doubling, often fiber crops middle part by tail place fresh hide binding, with between be connected with the fresh hide of binding, arrange in order, arrange tightly; General each retted fibre pond is thrown in all in batches, is convenient to wash fiber crops in batches; Throw in good fiber crops after then by fiber crops being all pressed in water, substantially do not surface and be advisable.Finally use thicker plastics film (transparent) by all numb covering, then compress plastics film, in case numb skin is dried up.Fiber crops time spacing 15-20 centimetre.Fiber crops layer thickness is generally advisable at 2-2.5 centimetre.
(5) use spraying process to enter retted fibre pond the zymocyte liquid of preparation and do retted fibre process, obtaining after retted fibre can for textile fabrics bluish dogbane or jute fibre, and retted fibre condition is natural temperature, pH nature; The technique of spraying process is specially every 48 hours to retted fibre region sprinkling bacterium liquid 8ml.
Complete the retting time: look temperature fixed, general mid or late September, the retting time extended gradually to mid or late October, if temperature Change is little, then the retting time is substantially identical.10-20 days is about in bluish dogbane or jute normal harvest season.
Claims (3)
1. a Michaelis VitB1 genus bacillus DY3 bacterial strain, is characterized in that: its 16Sr DNA sequence dna is as shown in SEQ ID NO:1.
2. an application for Michaelis VitB1 genus bacillus DY3 bacterial strain as claimed in claim 1, is characterized in that: described bacterial strain is applied to retted fibre and prepares bastose or jute fibre.
3. the application of a kind of Michaelis VitB1 genus bacillus DY3 bacterial strain according to claim 2, is characterized in that: described bastose or jute fibre are applied to and make gunnysack, burlap, papermaking, rope, weavening carpet or curtain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410333874.9A CN104277995B (en) | 2014-07-14 | 2014-07-14 | Aneurinibacillus migulanus DY3 strain and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410333874.9A CN104277995B (en) | 2014-07-14 | 2014-07-14 | Aneurinibacillus migulanus DY3 strain and application thereof |
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| Publication Number | Publication Date |
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| CN104277995A true CN104277995A (en) | 2015-01-14 |
| CN104277995B CN104277995B (en) | 2017-01-11 |
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| CN201410333874.9A Expired - Fee Related CN104277995B (en) | 2014-07-14 | 2014-07-14 | Aneurinibacillus migulanus DY3 strain and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834176A (en) * | 2017-02-13 | 2017-06-13 | 南京工业大学 | A kind of nucleoside phosphorylase, encoding gene and its superior strain and application |
| CN109312298A (en) * | 2016-06-01 | 2019-02-05 | 瓦伦塔制药有限责任公司 | Michaelis thiamine Bacillus strain and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102557766A (en) * | 2012-02-21 | 2012-07-11 | 浙江大学 | Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water |
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2014
- 2014-07-14 CN CN201410333874.9A patent/CN104277995B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102557766A (en) * | 2012-02-21 | 2012-07-11 | 浙江大学 | Application of Paenibacillus macerans strain in dissolution of phosphorus source in soil or eutrophic water |
Non-Patent Citations (3)
| Title |
|---|
| KORAWIT CHAISU ET AL.: "Optimization of Poly Lactic Acid (PLA) Plastic Degradation by Aneurinibacillus Migulanus Using Response Surface Methodology", 《2012 INTERNATIONAL CONFERENCE ON BIOLOGICAL AND LIFE SCIENCES》, vol. 40, 31 December 2012 (2012-12-31) * |
| SOMEN ACHARYA ET AL.: "Alkaline cellulase produced by a newly isolated thermophilic Aneurinibacillus thermoaerophilus WBS2 from hot spring,India", 《AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH》, vol. 6, no. 26, 12 July 2012 (2012-07-12) * |
| 丁若垚: "亚麻粗砂脱胶微生物的选育与应用", 《中国博士学位论文全文数据库2014 工程科技Ⅰ辑》, no. 05, 15 May 2014 (2014-05-15) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109312298A (en) * | 2016-06-01 | 2019-02-05 | 瓦伦塔制药有限责任公司 | Michaelis thiamine Bacillus strain and application thereof |
| CN109312298B (en) * | 2016-06-01 | 2022-05-27 | 瓦伦塔制药有限责任公司 | Thiamine miehei bacillus strain and application thereof |
| CN106834176A (en) * | 2017-02-13 | 2017-06-13 | 南京工业大学 | A kind of nucleoside phosphorylase, encoding gene and its superior strain and application |
| CN106834176B (en) * | 2017-02-13 | 2020-05-05 | 南京工业大学 | Nucleoside phosphorylase, coding gene, high-yield strain thereof and application |
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| CN104277995B (en) | 2017-01-11 |
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