CN104178821B - A kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre - Google Patents
A kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre Download PDFInfo
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- CN104178821B CN104178821B CN201410333921.XA CN201410333921A CN104178821B CN 104178821 B CN104178821 B CN 104178821B CN 201410333921 A CN201410333921 A CN 201410333921A CN 104178821 B CN104178821 B CN 104178821B
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- alcaligenes faecalis
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Abstract
The present invention relates to a kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre, comprising: the Alcaligenes faecalis DY1 bacterial strain preserved is cultivated by (1) in beef-protein medium, adds frozen damping fluid; (2) in jute bran mass, bacterium one ring of step (1) gained is accessed, shake-flask culture; (3) culture medium inoculated of step (2) gained being entered jute wheat bran fermention medium, shake-flask culture obtains zymocyte liquid; (4) retted fibre pond is made; (5) zymocyte liquid of preparation in step (3) uses spraying process send into retted fibre pond and do retted fibre process, obtained jute fibre after retted fibre. Technique of the present invention is simple, Production Flow Chart is short, be applicable to large-scale industrial production.
Description
Technical field
The invention belongs to retted fibre field, in particular to a kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre.
Background technology
Fiber crops are one of textile raw materials, and its kind mainly contains flax, ramie, hemp, bluish dogbane, jute, kendir, sisal hemp, abaca, nettle and piemarker etc. Flaxen fiber is the general designation of the fiber obtained from various fiber crops plant, the advantage that other natural fibers such as having moisture absorption, breathe freely, dispel the heat and be antibacterial are incomparable. China is as one of the abundantest country of hemp resource in the world, and the annual flaxen fiber produced and goods thereof, except the demand meeting domestic market, also as the export of commodities in large amounts U.S., West Europe and country in Southeast Asia, earn foreign exchange nearly 10,000,000,000 dollars every year. Country's energy-saving and emission-reduction policy and human consumer's back to nature, pursue green consumption idea and promote under, bast-fibre green processing technology has become the research focus of field of textiles.
Retted fibre utilizes bacterium and moisture that the effect of plant, dissolving or corrosion are enclosed in the most of cellular reticular tissue outside bast fibre bundle and colloid, thus impels the course of processing that fiber is separated with fiber crops stem. In the bubble method of widespread use, fiber crops stem is immersed in water, and water permeation, to the middle body of fiber crops stem, makes inner cell expansion, bursts outermost layer, thus increase the absorption of moisture and bacterium. Process of retting flax with is the complete processing producing good fiber quality, and the bacterial classification selection of retted fibre process is the key of retted fibre.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre, and the method is simple, Production Flow Chart is short, be applicable to large-scale industrial production.
A kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre of the present invention, comprising:
(1) the Alcaligenes faecalis DY1 bacterial strain preserved is cultivated in beef-protein medium, add frozen damping fluid;
(2) in jute bran mass, bacterium one ring of step (1) gained is accessed, shake-flask culture; The add-on of bacterium one ring is that every 1000ml bacterium liquid adds 200mg;
(3) culture medium inoculated of step (2) gained being entered jute wheat bran fermention medium, shake-flask culture obtains zymocyte liquid;
(4) retted fibre pond is made;
(5) zymocyte liquid of preparation in step (3) uses spraying process send into retted fibre pond and do retted fibre process, obtained jute fibre after retted fibre.
The 16SrDNA sequence of the Alcaligenes faecalis DY1 bacterial strain in described step (1) is as shown in SEQIDNO:1.
Consisting of of beef-protein medium in described step (1): every 1000ml substratum comprises extractum carnis 3g, peptone 10g, sodium-chlor 5g and water 1000ml.
Consisting of of frozen damping fluid in described step (1): every frozen damping fluid of 100ml comprises glycerine 50ml and water 50ml; Add-on is that every 1000ml bacterium liquid adds 1ml.
Bacterial strain preservation condition in described step (1) is-80 DEG C, pH nature; Culture condition is 37 DEG C, pH nature, incubation time 12 hours.
Consisting of of jute bran mass in described step (2): every 1000ml substratum comprises jute skin 200g, wheat bran 50g, wort 50ml and water 950ml; Consisting of of jute wheat bran fermention medium in step (3): every 1000ml substratum comprises jute skin 100g, wheat bran 100g, wort 200ml and water 800ml, and inoculum size is that every 1000ml bacterium liquid is inoculated into 30L jute wheat bran fermention medium.
Culture condition in described step (2) and (3) is 37 DEG C, pH nature, incubation time 24 hours.
The fiber crops in the retted fibre pond in described step (4) plow spacing and are 15-20 centimetre, and fiber crops layer thickness is 2-2.5 centimetre.
Retted fibre condition in described step (5) is natural temperature (about 20-30 degree Celsius), pH nature, 10-20 days retted fibre time; The technique of spraying process is specially every 48 hours to retted fibre region sprinkling bacterium liquid 7��9ml.
Obtained jute fibre in described step (5) is applied to and makes gunnysack, burlap, papermaking, rope, weavening carpet or curtain.
The preserving number of Alcaligenes faecalis DY1 bacterial strain of the present invention is CGMCCNo.9119, and preservation day is on May 4th, 2014, depositary institution CGMCC, it is proposed that classification called after: Alcaligenes faecalis (Alcaligenesfaecalis).
It is 99% according to check order Blast and cluster analysis result, this bacterium and Alcaligenes faecalis similarity of 16SrDNA, in conjunction with mirror inspection analysis, names this bacterial strain to be Alcaligenes faecalis (Alcaligenesfaecalis) DY1 bacterial strain. During 16SrDNA checks order, the forward primer 27F adopted, reverse primer 1492R, be international primer, synthesize by Shanghai Sangon Biological Engineering Technology And Service Co., Ltd.
This strain culturing condition is extensive, good heat resistance, and growth and breeding is fast, it is possible to effectively remove the non-cellulose colloid in jute fibre, and enzyme is lived stable, bacterium, the equal nontoxicity of enzyme. This bacterial strain and culture systems can be directly used in jute retted fibre.
Useful effect
(1) bacterial strain of the present invention and culture systems, jute retted fibre can be directly used in after testing, having the retted fibre cycle short, the features such as retted fibre effect is good, bacterial classification is not easily contaminated, processing cost is low, fiber quality is good, retted fibre mild condition, and contamination resistance is strong, resistance toheat is good, the advantages such as non-environmental-pollution;
(2) preparation method of the present invention has the features such as simple, the applicable large-scale industrial production of technique; Utilize this system to carry out flax retting and there is retted fibre time short-sighted weather condition, the features such as (normal harvest season) that generally can shorten 2-3 days.
Accompanying drawing explanation
Fig. 1 is bacterial strain uses therefor microscope stained photographs of the present invention (oil mirror);
Fig. 2 a-j is the DNA of bacteria collection of illustrative plates that the present invention adopts primer 2 7F to produce;
Fig. 3 a-j is the DNA of bacteria collection of illustrative plates that the present invention adopts primer 1492R to produce.
Embodiment
Below in conjunction with specific embodiment, set forth the present invention further. Limit the scope of the invention it will be understood that these embodiments are only not used in for illustration of the present invention. In addition it will be understood that after having read the content that the present invention lectures, the present invention can be made various changes or modifications by those skilled in the art, and these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
The retted fibre time is fixed according to jute harvest time, is generally mid or late September to mid or late October.
Retting flax method:
(1) the Alcaligenes faecalis DY1 bacterial strain preserved is cultivated in beef-protein medium (every 1000ml substratum comprises extractum carnis 3g, peptone 10g, sodium-chlor 5g and water 1000ml), add frozen damping fluid (the frozen damping fluid of every 100ml comprises glycerine 50ml and water 50ml), culture condition is 37 DEG C, pH nature, incubation time 12 hours;
(2) accessing bacterium one ring, shake-flask culture in jute bran mass (every 1000ml substratum comprises jute skin 200g, wheat bran 50g, wort 50ml and water 950ml) 1000ml, culture condition is 37 DEG C, pH nature, incubation time 24 hours;
(3) culture medium inoculated is entered jute wheat bran fermention medium (every 1000ml substratum comprises jute skin 100g, wheat bran 100g, wort 200ml and water 800ml), shake-flask culture obtains zymocyte liquid, culture condition is 37 DEG C, pH nature, incubation time 24 hours;
(4) preparation in retted fibre pond: with 4HB-800 type Huang/red ramie peeler fresh hide is separated from the full bar of jute (phloem because of mechanical presses, break plate and hit to beat and caused many wounds, be beneficial to degumming bacterium invasion, but do not destroy fiber); Aft end is neat (can be got rid of as degree during to wash fiber crops, about 20 is one), ending holds together together, straight, fold becomes three to four layers, and (retted fibre tank depth is similar, numb is just flooded during immersion) or doubling, often fiber crops middle part by tail place fresh hide binding, with between is connected with the fresh hide of binding, arrange in order, row tightly; Each retted fibre pond general is thrown in all in batches, is convenient to wash fiber crops in batches; Throw in good fiber crops after then by fiber crops being all pressed in water, substantially do not surface and be advisable. Finally with thicker plastics film (transparent) by all numb covering, then compress plastics film, in case fiber crops skin is dried up. Fiber crops time spacing 15-20 centimetre. Fiber crops layer thickness is generally advisable at 2-2.5 centimetre.
(5) zymocyte liquid of preparation using spraying process enter retted fibre pond and do retted fibre process, obtaining after retted fibre can for textile fabrics jute fibre, and retted fibre condition is natural temperature, pH nature; The technique of spraying process is specially every 48 hours to retted fibre region sprinkling bacterium liquid 8ml.
Completing the retting time: looking temperature fixed, general mid or late September to mid or late October, the retting time extends gradually, if temperature Change is little, then the retting time is substantially identical. It is about 10-20 days at jute normal harvest season.
Claims (10)
1. utilize Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare a method for jute fibre, comprising:
(1) the Alcaligenes faecalis DY1 bacterial strain preserved is cultivated in beef-protein medium, add frozen damping fluid;
(2) in jute bran mass, bacterium one ring of step (1) gained is accessed, shake-flask culture;
(3) culture medium inoculated of step (2) gained being entered jute wheat bran fermention medium, shake-flask culture obtains zymocyte liquid;
(4) retted fibre pond is made;
(5) zymocyte liquid of preparation in step (3) uses spraying process send into retted fibre pond and do retted fibre process, obtained jute fibre after retted fibre.
2. a kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre according to claim 1, it is characterised in that: the 16SrDNA sequence of the Alcaligenes faecalis DY1 bacterial strain in described step (1) is as shown in SEQIDNO:1.
3. a kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre according to claim 1, it is characterised in that: consisting of of the beef-protein medium in described step (1): every 1000ml substratum comprises extractum carnis 3g, peptone 10g, sodium-chlor 5g and water 1000ml.
4. a kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre according to claim 1, it is characterised in that: consisting of of the frozen damping fluid in described step (1): every frozen damping fluid of 100ml comprises glycerine 50ml and water 50ml; Add-on is that every 1000ml bacterium liquid adds 1ml.
5. a kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre according to claim 1, it is characterised in that: the bacterial strain preservation condition in described step (1) is-80 DEG C, pH nature; Culture condition is 37 DEG C, pH nature, incubation time 12 hours.
6. a kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre according to claim 1, it is characterized in that: consisting of of the jute bran mass in described step (2): every 1000ml substratum comprises jute skin 200g, wheat bran 50g, wort 50ml and water 950ml; Consisting of of jute wheat bran fermention medium in step (3): every 1000ml substratum comprises jute skin 100g, wheat bran 100g, wort 200ml and water 800ml, and inoculum size is that every 1000ml bacterium liquid is inoculated into 30L jute wheat bran fermention medium.
7. a kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre according to claim 1, it is characterised in that: the culture condition in described step (2) and (3) is 37 DEG C, pH nature, incubation time 24 hours.
8. a kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre according to claim 1, it is characterised in that: the fiber crops in the retted fibre pond in described step (4) plow spacing and are 15-20 centimetre, and fiber crops layer thickness is 2-2.5 centimetre.
9. a kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre according to claim 1, it is characterised in that: the retted fibre condition in described step (5) is natural temperature, pH nature, 10-20 days retted fibre time; The technique of spraying process is specially every 48 hours to retted fibre region sprinkling bacterium liquid 7��9ml.
10. a kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre according to claim 1, it is characterised in that: the obtained jute fibre in described step (5) is applied to and makes gunnysack, burlap, papermaking, rope, weavening carpet or curtain.
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| CN201410333921.XA CN104178821B (en) | 2014-07-14 | 2014-07-14 | A kind of method utilizing Alcaligenes faecalis DY1 bacterial strain retted fibre to prepare jute fibre |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2181797C2 (en) * | 2000-06-19 | 2002-04-27 | Костромская государственная сельскохозяйственная академия | Method to obtain dew-retting treated plant fibers of fiber flax under controlled conditions |
| CN101285211A (en) * | 2008-06-05 | 2008-10-15 | 黑龙江省科学院亚麻综合利用研究所 | Microorganism retting flax method for crudefiber crops |
| CN101654660A (en) * | 2009-07-14 | 2010-02-24 | 东华大学 | Bacillus subtillis strain containing ramie degumming activity, preparation and application thereof |
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2014
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2181797C2 (en) * | 2000-06-19 | 2002-04-27 | Костромская государственная сельскохозяйственная академия | Method to obtain dew-retting treated plant fibers of fiber flax under controlled conditions |
| CN101285211A (en) * | 2008-06-05 | 2008-10-15 | 黑龙江省科学院亚麻综合利用研究所 | Microorganism retting flax method for crudefiber crops |
| CN101654660A (en) * | 2009-07-14 | 2010-02-24 | 东华大学 | Bacillus subtillis strain containing ramie degumming activity, preparation and application thereof |
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