CN104232505A - Lysinibacillus sp. DY2 bacterial strain and application thereof - Google Patents
Lysinibacillus sp. DY2 bacterial strain and application thereof Download PDFInfo
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- CN104232505A CN104232505A CN201410334118.8A CN201410334118A CN104232505A CN 104232505 A CN104232505 A CN 104232505A CN 201410334118 A CN201410334118 A CN 201410334118A CN 104232505 A CN104232505 A CN 104232505A
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Abstract
The invention relates to a lysinibacillus sp. DY2 bacterial strain and an application thereof. The 16Sr DNA sequence is shown in SEQ ID NO:1. The bacterial strain is applied to preparing hibiscus cannabinus fibers or jute fibers by retting. The bacterial strain provided by the invention is extensive in culture condition, good in heat resistance and fast in growth and propagation, can be used for effectively removing non-cellulose colloids in hibiscus cannabinus fibers or jute fibers, is stable in enzyme activity and non-toxic in bacteria and enzymes and has a good application prospect.
Description
Technical field
The invention belongs to retted fibre field, particularly a kind of Methionin genus bacillus DY2 bacterial strain and application thereof.
Background technology
Fiber crops are one of textile raw materials, and its kind mainly contains flax, ramie, hemp, bluish dogbane, jute, kendir, sisal hemp, abaca, nettle and piemarker etc.Flaxen fiber is the general designation of the fiber obtained from various numb plant, has the advantage that moisture absorption, ventilative, heat radiation and other natural fibers such as antibacterial are incomparable.China is as one of the abundantest country of hemp resource in the world, and the annual flaxen fiber produced and goods thereof, except the demand meeting domestic market, also as the export of commodities in large amounts U.S., West Europe and country in Southeast Asia, earn foreign exchange nearly 10,000,000,000 dollars every year.Reduce discharging policy and human consumer's back to nature at national energy-saving, pursue under green consumption idea promotes, bast-fibre green processing technologies has become the study hotspot of field of textiles.
Retted fibre utilizes bacterium and moisture to the effect of plant, dissolves or corrodes the cellular reticular tissue of major part and colloid that are enclosed in outside bast fibre bundle, thus impelling the course of processing that fiber is separated with straw.In the bubble method of widespread use, straw is immersed in water, and water permeation, to the middle body of straw, makes inner cell expansion, bursts outermost layer, thus increases the absorption to moisture and bacterium.Process of retting flax with is the complete processing of producing good fiber quality, and the bacterial classification selection of retted fibre process is the key of retted fibre.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Methionin genus bacillus DY2 bacterial strain and application thereof, this strain culturing condition is extensive, good heat resistance, growth and breeding is fast, effectively can remove the non-cellulose colloid in bluish dogbane or jute fibre, and enzyme is lived stable, bacterium, the equal nontoxicity of enzyme, have a good application prospect.
A kind of Methionin genus bacillus DY2 bacterial strain of the present invention, its 16Sr DNA sequence dna is as shown in SEQ ID NO:1.
The application of a kind of Methionin genus bacillus DY2 bacterial strain of the present invention, described bacterial strain is applied to retted fibre and prepares bastose or jute fibre.
Described bastose or jute fibre are applied to and make gunnysack, burlap, papermaking, rope, weavening carpet or curtain.
The preserving number of Methionin genus bacillus DY2 bacterial strain of the present invention is CGMCC No.9120, preservation day is on May 4th, 2014, suggestion Classification And Nomenclature is: Methionin genus bacillus (Lysinibacillus sp.), depositary institution's title: CGMCC China Committee for Culture Collection of Microorganisms common micro-organisms center, preservation address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica.
According to 16sR DNA sequencing Blast and cluster analysis result, this bacterium and Methionin genus bacillus similarity are 99%, in conjunction with microscopy analysis, name this bacterial strain to be Methionin genus bacillus (Lysinibacillus sp.) DY2 bacterial strain.During 16S rDNA checks order, the forward primer 27F adopted, reverse primer 1492R, be international primer, synthesized by Shanghai Sangon Biological Engineering Technology And Service Co., Ltd.
beneficial effect
Culture condition of the present invention is extensive, good heat resistance, and growth and breeding is fast, effectively can remove the non-cellulose colloid in bluish dogbane or jute fibre, and enzyme is lived stable, and bacterium, the equal nontoxicity of enzyme, have a good application prospect.
Accompanying drawing explanation
Fig. 1 is bacterial strain uses therefor microscope stained photographs (oily mirror) of the present invention;
Fig. 2 a-j is the DNA of bacteria collection of illustrative plates that the present invention adopts primer 2 7F to produce;
Fig. 3 a-j is the DNA of bacteria collection of illustrative plates that the present invention adopts primer 1492R to produce.
Embodiment
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after the content of having read the present invention's instruction.
Embodiment 1
The retted fibre time determines according to bluish dogbane or jute harvest time, is generally mid or late September to mid or late October.
Retting flax method:
(1) the Methionin genus bacillus DY2 bacterial strain preserved is cultivated in beef-protein medium (every 1000ml substratum comprises extractum carnis 3g, peptone 10g, sodium-chlor 5g and water 1000ml), add frozen damping fluid (the frozen damping fluid of every 100ml comprises glycerine 50ml and water 50ml), culture condition is 37 DEG C, pH nature, incubation time 12 hours;
(2) in bluish dogbane or jute bran mass (every 1000ml substratum comprises bluish dogbane or jute skin 200g, wheat bran 50g, wort 50ml and water 950ml) 1000ml, bacterium one ring is accessed, shake-flask culture, culture condition is 37 DEG C, pH nature, incubation time 24 hours;
(3) (every 1000ml substratum comprises bluish dogbane or jute skin 100g culture medium inoculated to be entered bluish dogbane or jute wheat bran fermention medium, wheat bran 100g, wort 200ml and water 800ml), shake-flask culture obtains zymocyte liquid, culture condition is 37 DEG C, pH nature, incubation time 24 hours;
(4) preparation in retted fibre pond: with 4HB-800 type bluish dogbane or jute peeling machine fresh hide is separated from bluish dogbane or the full bar of jute (phloem because of mechanical presses, re-pack impact caused many wounds, be beneficial to degumming bacterium invasion, but do not destroy fiber); Afterbody end is neat (can be got rid of as degree during to wash fiber crops, about 20 is one), ending is held together together, straight, fold becomes three to four layers, and (retted fibre tank depth is similar, numb is just in time flooded during immersion) or doubling, often fiber crops middle part by tail place fresh hide binding, with between be connected with the fresh hide of binding, arrange in order, arrange tightly; General each retted fibre pond is thrown in all in batches, is convenient to wash fiber crops in batches; Throw in good fiber crops after then by fiber crops being all pressed in water, substantially do not surface and be advisable.Finally use thicker plastics film (transparent) by all numb covering, then compress plastics film, in case numb skin is dried up.Fiber crops time spacing 15-20 centimetre.Fiber crops layer thickness is generally advisable at 2-2.5 centimetre.
(5) use spraying process to enter retted fibre pond the zymocyte liquid of preparation and do retted fibre process, obtaining after retted fibre can for textile fabrics bluish dogbane or jute fibre, and retted fibre condition is natural temperature, pH nature; The technique of spraying process is specially every 48 hours to retted fibre region sprinkling bacterium liquid 8ml.
Complete the retting time: look temperature fixed, general mid or late September, the retting time extended gradually to mid or late October, if temperature Change is little, then the retting time is substantially identical.10-20 days is about in bluish dogbane or jute normal harvest season.
Claims (3)
1. a Methionin genus bacillus DY2 bacterial strain, is characterized in that: its 16Sr DNA sequence dna is as shown in SEQ ID NO:1.
2. an application for Methionin genus bacillus DY2 bacterial strain as claimed in claim 1, is characterized in that: described bacterial strain is applied to retted fibre and prepares bastose or jute fibre.
3. the application of a kind of Methionin genus bacillus DY2 bacterial strain according to claim 2, is characterized in that: described bastose or jute fibre are applied to and make gunnysack, burlap, papermaking, rope, weavening carpet or curtain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410334118.8A CN104232505B (en) | 2014-07-14 | A kind of lysine bacillus cereus DY2 bacterial strain and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410334118.8A CN104232505B (en) | 2014-07-14 | A kind of lysine bacillus cereus DY2 bacterial strain and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104232505A true CN104232505A (en) | 2014-12-24 |
| CN104232505B CN104232505B (en) | 2017-01-04 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105567605A (en) * | 2016-02-19 | 2016-05-11 | 黑龙江省科学院大庆分院 | Lysinibacillus sp. and application thereof, degumming auxiliary containing lysinibacillus sp. and preparation method of degumming auxiliary |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103074275A (en) * | 2012-12-27 | 2013-05-01 | 江南大学 | Lysinibacillus fusiformis for producing ethyl urethane hydrolase and application of lysinibacillus fusiformis |
| CN103910435A (en) * | 2014-01-14 | 2014-07-09 | 暨南大学 | Use of Lysinibacillus sp. FS1 in dye degradation |
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103074275A (en) * | 2012-12-27 | 2013-05-01 | 江南大学 | Lysinibacillus fusiformis for producing ethyl urethane hydrolase and application of lysinibacillus fusiformis |
| CN103910435A (en) * | 2014-01-14 | 2014-07-09 | 暨南大学 | Use of Lysinibacillus sp. FS1 in dye degradation |
Non-Patent Citations (4)
| Title |
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| LIU B 等: "KJ000805.1", 《GENBANK》 * |
| 孙庆祥 等: "黄麻和红麻脱胶细菌的分离和初步鉴定", 《中国麻作》 * |
| 葛菁萍 等: "沤麻用果胶酶产生菌的选育及脱胶过程中细菌种群的初步分析", 《黑龙江大学自然科学学报》 * |
| 蔡柏岩 等: "大麻沤麻液细菌种群多样性", 《生态学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105567605A (en) * | 2016-02-19 | 2016-05-11 | 黑龙江省科学院大庆分院 | Lysinibacillus sp. and application thereof, degumming auxiliary containing lysinibacillus sp. and preparation method of degumming auxiliary |
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Granted publication date: 20170104 Termination date: 20190714 |