CN105712490A - Method for treating high ammonia-nitrogen wastewater through nutrition conversion of mixotroph - Google Patents
Method for treating high ammonia-nitrogen wastewater through nutrition conversion of mixotroph Download PDFInfo
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a method for treating high ammonia-nitrogen wastewater through nutrition conversion of mixotroph. The method for treating the high ammonia-nitrogen wastewater through nutrition conversion of the mixotroph comprises the following steps: 1, conducting transferring and culture on the mixotroph; 2, conducting high density culture in a culture medium rich in organic carbon or wastewater rich in organic carbon; 3, harvesting heterotrophic cells of the mixotroph; 4, transferring the heterotrophic cells into the high ammonia-nitrogen wastewater for autotrophic culture, absorbing high-concentration nitrogen and ammonia, and purifying the wastewater. According to the method, the method for changing the nutrition metabolism pathway of the mixotroph is introduced for treating the high ammonia-nitrogen wastewater, the treated wastewater can be recycled, the requirement of industrialized wastewater treatment conducted through microalgae is met, and the method is a new approach for conducting sewage treatment through produced microalgae economically and efficiently. The harvested microalgae cells can be further treated and used for preparing biological energy source, animal feed and the like.
Description
Technical field
The present invention relates to the processing method of high ammonia-nitrogen wastewater, in particular to a kind of method of mixotroph nutrition conversion processing high ammonia-nitrogen wastewater.
Background technology
Water resources crisis is the significant challenge of 21 century facing mankind.Along with global industry, modern fast development, human society grows with each passing day for the demand of water resource.But, owing to the activity of the mankind causes that the severe contamination of water environment, restriction even hinder social development and the progressive impetus.
High ammonia nitrogen (NH4 +-N) treatment and purification of waste water is a great problem of whole world wastewater treatment, the such as animal husbandry of many industrial and agricultural production departments, chemical fertilizer industry, explosive industry, refuse landfill etc., all has a large amount of high ammonia-nitrogen wastewater to discharge.If effectively not processed, such waste water can cause a series of serious environmental problems such as body eutrophication, stench, soil degradation, underground water pollution.NH4 +-N can be removed by multiple physics, chemistry, biological method, and bioanalysis, due to less costly and environment side effect is less, becomes focus in the industry in recent decades.So far, it is most widely used by the activated sludge process of nitrification-denitrification process denitrogenation, but the construction of the construction cost of this method and aerator and operation all need to put into great amount of cost, in addition, the C/N of such waste water is than the suitableeest process range being generally significantly less than activated sludge process, additionally not adding organic carbon ammonia nitrogen removal cannot be up to standard, and adding carbon source can increase again the processing cost of waste water.
Research both at home and abroad shows, adopts mixotroph such as microalgae to process waste water and can overcome drawbacks described above.Due to NH4 +-N is the nitrogenous source form the most easily utilized by most of microalgae, and therefore the activated sludge process than based on antibacterial on ammonia nitrogen removal of the sewage disposal system based on microalgae has a clear superiority in.The introducing of immobilization cell or simple microalgae system, becomes the new approaches solving traditional active sludge process high high ammonia-nitrogen wastewater limitation in recent years.The removal pathway of ammonia nitrogen is included by the process system based on microalgae: (1) microalgae photosynthesis produces oxygen and promotes nitrification-denitrification process；(2) microalgae causes pH value of waste water to raise in photosynthesis, makes NH4 +-N volatilizees with the form of free ammonia；(3) system stirring, mixing cause that free ammonia volatilizees (also referred to as strengthening stripping effect) further；(4) NH4 +-N is adsorbed by microalgae cell, absorbs.But in said system, generally only accounted for microalgae by the ammonia nitrogen of microalgae cell absorption, absorption and process the sub-fraction of system, a large amount of ammonia spill into and can cause potential atmospheric pollution in air.For detecting high NH4 +During-N wastewater treatment, by the NH of the actual absorption of microalgae cell, absorption4 +-N measures, and need to adopt sterilizing and the water distribution of regulation and control pH value, and to get rid of bacterial nitrification-Denitrification, pH value raises, stir the impact mixing other microorganisms such as the free ammonia volatilization stripping caused and antibacterial.
Summary of the invention
Present invention aim to the deficiency overcome existing for prior art, it is provided that a kind of mixotroph nutrition converts the method processed for high ammonia-nitrogen wastewater.
For achieving the above object, the mixotroph nutrition designed by the present invention converts the method processed for high ammonia-nitrogen wastewater, carries out according to following steps:
1) switching of mixotroph and cultivation: inoculate in-70 DEG C of refrigerators mixotroph bacterial strain and with inoculating loop scraping a little to solid slope flat board illumination autotrophy, raise together with or Heterotrophic culture；
Described autotrophy condition of culture is as follows: temperature is 20～45 DEG C, with 28 DEG C for best；The initial concentration of illumination cultivation nitrogenous source is 1～15g/L, it is preferable that 4g/L, and nitrogenous source is glycine or yeast extract；Pass into air or air and CO2Mixing gas, ventilation 50～300L/h, it is preferable that 80～120L/h, CO2Concentration 0.9～3%；Incubation adopts 10～200 μm of ol/m2The sun exposure of/s, pH value controls, between 5～9, to be preferred with 7.0；Total incubation time visual cell growing state and determine, be typically in the range of 50～400 hours, it is preferable that 120～200 hours；
Described raise together with as follows with Heterotrophic culture condition: pass into air, ventilation 100～400L/h, it is preferable that 150～250L/h；Incubation adopts 5～40 μm of ol/m2The sun exposure of/s, pH value controls, between 5～9, to be preferred with 7.0；Total incubation time visual cell growing state and determine, be typically in the range of 72～200 hours, it is preferable that 100～150 hours；Wherein, Heterotrophic culture adds different organic carbon source in the medium is 0.1～200g/L to initial reduction sugar concentration, it is preferred to 15～20g/L；Raising together with cultivation and add different organic carbon sources and inorganic carbon source in the medium, organic carbon source is 0.1～200g/L to initial reduction sugar concentration, it is preferred to 15～20g/L, and inorganic carbon source is mass fraction 0.001%～100%CO2, optimum 2%, or concentration is the NaHCO of 0.1～100g/L3Or Na2CO3Or both mixing, optimum 0.2g/L；
2) rich in organic carbon culture medium or rich in the cultivation of machine carbon waste water middle-high density: plate culture is seeded to biological reaction apparatus rich in organic carbon culture medium or rich in machine carbon waste water middle-high density Heterotrophic culture, until cell log trophophase cell density is reaching 106～1010；Heterotrophic culture condition is as follows: adding different organic carbon source in the medium is 0.1～200g/L to initial reduction sugar concentration, passes into air, ventilation 100～400L/h, it is preferable that 150-250L/h；Incubation adopts 5～40 μm of ol/m2The sun exposure of/s, pH value controls, between 5～9, to be preferred with 7.0；Total incubation time visual cell growing state and determine, be typically in the range of 72～200 hours, it is preferable that 100～150 hours；
3) results of mixotroph heterotrophic cell: the heterotrophic cell of take the logarithm Later growth or stable phase, harvested by centrifugation under the slow-speed of revolution of 2000～8000r/min, the mixotroph heterotrophic cell of results is with aseptic water washing twice, and then results；
4) it is transferred to high ammonia-nitrogen wastewater and carries out autotrophy cultivation, absorb ammonia nitrogen in high density and purify waste water: the mixotroph heterotrophic cell of results being added high ammonia-nitrogen wastewater carries out autotrophy cultivation, the volume ratio of mixotroph heterotrophic cell and high ammonia-nitrogen wastewater is 1:1～1:100, nutrition conversion process needs absorb substantial amounts of high ammonia nitrogen nutrient for synthesizing chloroplast associated component and the various enzymes relevant with photosynthesis, thus reaching to purify the purpose of waste water；
Described autotrophy condition of culture is as follows: temperature is 20～45 DEG C, with 28 DEG C for best；Illumination cultivation nitrogenous source is ammonia nitrogen, by adding different amounts of NH4Cl regulates ammonia nitrogen initial concentration in high ammonia-nitrogen wastewater, makes ammonia nitrogen initial concentration between 40mg/L～160mg/L, wherein NH4The concentration of Cl is 40mg/L, 80mg/L and 160mg/L；Pass into the mixing gas of air or air and CO2, ventilation 50～300L/h, it is preferable that 80～120L/h；CO2 concentration 0.1～15%；Adopting the sun exposure of 10～200 μm of ol/m2/s in incubation, pH value controls, 5～9, to be preferred with 7.0；Total incubation time visual cell growing state and determine, be typically in the range of 4～400 hours, it is preferable that 12-48 hour；
Described high ammonia-nitrogen wastewater formula is as follows: NaCl0.007g/L, MgSO4·7H2O0.002g/L、CaCl2·2H2O0.004g/L、KH2PO40.0085g/L、K2HPO40.0217g/L、Na2HPO40.025g/L, Trace Metal solution 0.1ml/L, wherein Trace Metal solution composition: H3BO35.7g/L、MnCl2·4H2O2.5g/L、ZnSO4·7H2O11g/L、FeSO4·7H2O2.5g/L、Na2MoO4·2H2O0.15g/L、Na2EDTA25g/L and CoCl2·6H2O0.8g/L。
Step 1 of the present invention) in, described mixotroph is preferably microalgae or photosynthetic bacteria.
Step 2 of the present invention) in, described organic carbon source is preferably glucose, fructose, corn starch hydrolysate, tapioca hydrolysate, molasses, ribose, deoxyribose, ribulose, acetic acid, propanoic acid, butanoic acid or glycerol.Described organic carbon source initial reduction sugar concentration is 1～20g/L.
Step 2 of the present invention) in, described it is preferably sanitary wastewater, waste water of paper mill, molasses containing waste water, beer brewery water, dairy food processing plants waste water, biogas fermentation waste water or animal wastes waste water rich in organic carbon waste water.
In the present invention, described microalgae is preferably Chlorella, barrel mast Trentepohlia, diatom, rhombus algae, splits kettle algae, Dunaliella, scenedesmus, Nannochloropsis oceanica, Chlamydomonas, flat algae or empty ball Trentepohlia.
In the present invention, described microalgae includes but not limited to Chlorella (Chlorellasp.), barrel mast Trentepohlia (Cylindrothecasp.), diatom (Diatom), rhombus algae (Nitzschiasp.), splits kettle algae (schizochytriumsp.), Dunaliella (dunaliella), scenedesmus (Scenedesmussp.), Nannochloropsis oceanica (Nannochlorissp.), Chlamydomonas (chlamydomonassp.), flat algae (Tetraselmissp.), empty ball Trentepohlia (Eudorinasp.).
In the present invention, described photosynthetic bacteria includes but not limited to cyanobacteria (Cyanobacteria) and purple bacteria, described cyanobacteria (Cyanobacteria) belongs to (Synechococcus) or blue Pseudomonas (Oscillatoria) of quivering greatly selected from Synechococcus, and described purple bacteria is selected from Rhodospirillum (Rhodospirillum), Rhodopseudomonas (Rhodopseudomonas) or Rhodomicrobium (Rhodomicrobium).
In the present invention, High Density Cultivation is raised together with or Heterotrophic culture based formulas is: K2HPO4·3H2O0.04g/L、MgSO4·7H2O0.075g/L、CaCl2·2H2O0.036g/L, citric acid 0.006g/L, ferric ammonium citrate 0.006g/L, EDTA0.001g/L, NaNO31.5g/L、Na2CO30.02g/L and A5 liquid microelement 1.5ml/L；Wherein, A5 liquid microelement composition: H3BO32.86g/L、MnCl2·4H2O1.81g/L、ZnSO4·7H2O0.222g/L、NaMoO4·2H2O0.39g/L、CuSO4·5H2O0.079g/L and CoCl2·6H2O0.05g/L。
Autotrophy, three kinds of Model of Nutritions foster, that heterotrophism is microalgae of holding concurrently.Most microalgae can only autotrophy, part can carry out holding concurrently and supporting and heterotrophism.Autotrophy microalgae is with luminous energy for the energy, and DIC is carbon source, and heterotrophic microalgae can only with Organic substance for the energy and carbon source, and foster microalgae of holding concurrently then can carry out autotrophy and two kinds of Model of Nutritions of heterotrophism simultaneously, it may be assumed that both with luminous energy for the energy, again with Organic substance for the energy；Both with DIC for carbon source, again with Organic substance for carbon source.Compared with tradition autotrophy pattern, heterotrophism, the pattern of supporting of holding concurrently generally can realize dramatically increasing of Biomass within the relatively short time, and the utilization of luminous energy makes the double yield supporting pattern be bordering on or higher than heterotrophism pattern.Therefore, it is used for inoculating process high ammonia-nitrogen wastewater by heterotrophism, foster microalgae of holding concurrently, is all of value to raising system effectiveness.
The mixotroph that the present invention relates to belongs to can carry out the microorganism that photosynthesis can be able to act on again, volume is small in carrying out under dark condition under illumination condition, owing to mixotroph turns in autotrophy process at heterotrophism, need assembly such as various protein and enzyme etc. that substantial amounts of ammonia nitrogen synthesis chlorophyll and other photosynthesis need, it is thus possible to the ammonia nitrogen in high density in removal waste water, reach to purify the purpose of waste water.
The beneficial effects of the present invention is: the present invention develops a kind of biological high-concentration ammonia nitrogen waste water treatment method simply efficient, with low cost by mixotroph Nutrition and Metabolism approach.This technique can be used to meet heavy industrialization wastewater treatment further and be substantially reduced cost.
Invention introduces and change the method supporting abiotic Nutrition and Metabolism approach of holding concurrently, be used for processing high ammonia-nitrogen wastewater.The waste water processed can be recycled again, meets microalgae industryization and processes the requirement of wastewater application, is the new way of an economy, microalgae sewage disposal processed efficiently.The microalgae cell of results can process the preparation for bioenergy and animal feed etc. further.
Accompanying drawing explanation
Fig. 1 a is that chlorella in autotrophy and raises together with growth curve chart in culture medium.
Fig. 1 b is chlorella growth curve chart in Heterotrophic culture base.
The microscopic morphology that Fig. 2 is chlorella is observed.
Fig. 3 a, 3b, 3c, 3d are that mixotroph processes high ammonia-nitrogen wastewater design sketch.
Detailed description of the invention
In order to explain the present invention better, below in conjunction with the drawings and specific embodiments, the present invention is described in further detail, but the present invention is not constituted restriction by them.
Embodiment 1
The local screening of high ammonia nitrogen tolerance algae chlorella (Cholorellasorokiniana), this algae strain can at photosynthetic autophyting growth, again can at heterotrophic growth under dark condition, moreover it is possible to mixture growth under these conditions simultaneously.This chlorella is circular as can be seen from Figure 2, and cell size is the eucaryon mixotroph of 2～5 μm.Its autotrophy, heterotrophism and raise together with cultivate under growth characteristics as shown in Figure 1 a, 1 b.
Fig. 1 a represents chlorella and in autotrophy and raises together with growth curve chart in culture medium, and P represents autotrophy and cultivates, and M represents and raises together with cultivation, and M1-M50 represents and raises together with organic carbon concentration during cultivation (that is, organic carbon initial reduction sugar concentration).From Fig. 1 a it can be seen that raise together with cellular biomass in the identical time to be significantly larger than autotrophy culture biomass；And organic carbon concentration is directly proportional to raising together with Biomass.
Fig. 1 b represents chlorella growth curve chart in Heterotrophic culture base.Wherein H represents Heterotrophic culture, and H1-H50 represents organic carbon concentration during Heterotrophic culture.From Fig. 1 b it can be seen that in the identical time heterotrophic cell's Biomass be significantly larger than autotrophy culture biomass, suitable with raising together with Biomass；Organic carbon concentration is directly proportional to autotroph amount.
Therefore, adopt that to raise together with heterotrophism High Density Cultivation Rapid Accumulation micro algae biomass for next step experiment be very feasible.
1) switching of mixotroph and cultivation: inoculate chlorella cells in-70 DEG C of refrigerators, and a little to the activation algae strain of solid slope flat board illumination Heterotrophic culture by inoculating loop scraping, and heterotrophism condition is as follows: concentration of glucose 20g/L；Pass into air, ventilation 200L/h；Incubation adopts 30 μm of ol/m2The sun exposure of/s, pH value is 7；Total incubation time is 120 hours；
2) cultivating rich in organic carbon culture medium middle-high density: being cultivated to shaking table in 250mL shaking flask by the chlorella list colony inoculation being grown on solid medium, temperature controls at 28 DEG C ± 5 DEG C；After cell enters logarithmic growth latter stage, access and in biological reaction apparatus, carry out high Density Heterotrophic rich in organic carbon culture medium, biological reaction apparatus includes shaking flask, ventilation bottle, various bioreactor, fermentation tank and open culturing pond etc., Heterotrophic culture condition is as follows: adding glucose in the medium is 20g/L to initial reduction sugar concentration, pass into air, ventilation 220L/h；Incubation adopts 28 μm of ol/m2The sun exposure of/s, pH value is 7；Total incubation time is 130 hours, until cell log trophophase, cell density reaches 109；
3) results of mixotroph heterotrophic cell: the cell of the high Density Heterotrophic of take the logarithm Later growth or stable phase, 6000r/min slow-speed of revolution harvested by centrifugation, the mixotroph heterotrophic cell of results is with aseptic water washing twice, and then results；
4) it is transferred to high ammonia-nitrogen wastewater and carries out autotrophy cultivation, absorb ammonia nitrogen in high density and purify waste water: the mixotroph heterotrophic cell of results being added high ammonia-nitrogen wastewater carries out autotrophy cultivation, the volume ratio of mixotroph heterotrophic cell and high ammonia-nitrogen wastewater is 1:30, nutrition conversion process needs absorb substantial amounts of high ammonia nitrogen nutrient for synthesizing chloroplast associated component and the various enzymes relevant with photosynthesis, thus reaching to purify the purpose of waste water；
Autotrophy condition of culture is as follows: temperature is 28 DEG C；Illumination cultivation nitrogenous source is ammonia nitrogen, by adding different amounts of NH4Cl regulates ammonia nitrogen initial concentration in high ammonia-nitrogen wastewater, makes ammonia nitrogen initial concentration between 40mg/L～160mg/L, wherein NH4The concentration of Cl is 40mg/L, 80mg/L and 160mg/L；Pass into air or air and CO2Mixing gas, ventilation 120L/h；CO2Concentration 3%；Incubation adopts 150 μm of ol/m2The sun exposure of/s, pH value is 7, and total incubation time is 200 hours；
High ammonia-nitrogen wastewater formula is as follows: NaCl0.007g/L, MgSO4·7H2O0.002g/L、CaCl2·2H2O0.004g/L、KH2PO40.0085g/L、K2HPO40.0217g/L、Na2HPO40.025g/L, Trace Metal solution 0.1ml/L, wherein Trace Metal solution composition: H3BO35.7g/L、MnCl2·4H2O2.5g/L、ZnSO4·7H2O11g/L、FeSO4·7H2O2.5g/L、Na2MoO4·2H2O0.15g/L、Na2EDTA25g/L and CoCl2·6H2O0.8g/L。
Result is shown in the high ammonia-nitrogen wastewater that ammonia nitrogen initial concentration is 80mg/L ammonia nitrogen removal frank and reaches as high as 12.5mg/L/d；In the high ammonia-nitrogen wastewater that ammonia nitrogen initial concentration is 160mg/L, ammonia nitrogen removal frank reaches as high as 11.6mg/L/d and utilizes mixotroph nutrition Changing Strategy can efficiently remove high ammonia-nitrogen wastewater, as shown in Fig. 3 a, Fig. 3 b and Fig. 3 c.Wherein, NS, M is that rich nitrogen raises together with High Density Cultivation；NS, H are rich nitrogen heterotrophism High Density Cultivation；The rich nitrogen autotrophy of NS, P is cultivated；ND, the M nitrogen that is limited raises together with cultivation；ND, H are limited nitrogen Heterotrophic culture；ND, P are limited nitrogen autotrophy；80,160,240 is initial ammonia nitrogen concentration.
In the present embodiment, autotrophy culture medium prescription is: K2HPO4·3H2O0.04g/L、MgSO4·7H2O0.075g/L、CaCl2·2H2O0.036g/L, citric acid 0.006g/L, ferric ammonium citrate 0.006g/L, EDTA0.001g/L, NaNO31.5g/L、Na2CO30.02g/L and A5 liquid microelement 1.5ml/L；Wherein, A5 liquid microelement composition: H3BO32.86g/L、MnCl2·4H2O1.81g/L、ZnSO4·7H2O0.222g/L、NaMoO4·2H2O0.39g/L、CuSO4·5H2O0.079g/L and CoCl2·6H2O0.05g/L。
Ibid, and to add different organic carbon be 1～20g/L to initial reduction sugar concentration to Heterotrophic culture based formulas, optimum 15g/L.
Raising together with culture medium prescription ibid, and add different organic carbon and DIC, organic carbon is 1～20g/L to initial reduction sugar concentration, optimum 15g/L.DIC is 0.001%～100%CO2, optimum 2%, or DIC NaHCO3Or Na2CO3Or both mixing, concentration is 0.1～100g/L, optimum 0.2g/L.
The high ammonia-nitrogen wastewater that Nanchang ammonia nitrogen factory collects, its total nitrogen concentration is between 270～280mg/L, and ammonia nitrogen concentration is between 240～260mg/L.Processing method initially with the present invention is sequentially carried out High Density Cultivation and harvesting microalgae cell, then proceed to again in high ammonia-nitrogen wastewater and carry out autotrophy cultivation, result display adopts the method utilizing the rich nitrogen conversion process high ammonia-nitrogen wastewater of mixotroph of the present invention, in this high ammonia-nitrogen wastewater, ammonia nitrogen removal frank reaches as high as 12.3mg/L/d, as shown in Figure 3 d.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, being appreciated that and these embodiments can be carried out multiple change, amendment, replacement and modification without departing from the principles and spirit of the present invention, the scope of the present invention be defined by the appended.
Claims (8)
1. a mixotroph nutrition converts the method processed for high ammonia-nitrogen wastewater, it is characterised in that carry out according to following steps:
1) switching of mixotroph and cultivation: inoculate in-70 DEG C of refrigerators mixotroph bacterial strain and with inoculating loop scraping a little to solid slope flat board illumination autotrophy, raise together with or Heterotrophic culture；
Described autotrophy condition of culture is as follows: temperature is 20～45 DEG C；The initial concentration of illumination cultivation nitrogenous source is 1～15g/L, and nitrogenous source is glycine or yeast extract；Pass into air or air and CO2Mixing gas, ventilation 50～300L/h, CO2Concentration 0.9～3%；Incubation adopts 10～200 μm of ol/m2The sun exposure of/s, pH value controls between 5～9；Total incubation time is 50～400 hours；
Described raise together with as follows with Heterotrophic culture condition: pass into air, ventilation 100～400L/h；Incubation adopts 5～40 μm of ol/m2The sun exposure of/s, pH value controls between 5～9；Total incubation time is 72～200 hours；Wherein, Heterotrophic culture adds different organic carbon source in the medium is 0.1～200g/L to initial reduction sugar concentration；Raising together with cultivation and add different organic carbon sources and inorganic carbon source in the medium, organic carbon source is 0.1～200g/L to initial reduction sugar concentration, and inorganic carbon source is mass fraction 0.001%～100%CO2, or concentration is the NaHCO of 0.1～100g/L3Or Na2CO3Or both mixing；
2) rich in organic carbon culture medium or rich in the cultivation of machine carbon waste water middle-high density: plate culture is seeded to biological reaction apparatus rich in organic carbon culture medium or rich in machine carbon waste water middle-high density Heterotrophic culture, until cell log trophophase cell density is reaching 106～1010；Heterotrophic culture condition is as follows: adding different organic carbon source in the medium is 0.1～200g/L to initial reduction sugar concentration, passes into air, ventilation 100～400L/h；Incubation adopts 5～40 μm of ol/m2The sun exposure of/s, pH value controls 5～9；Total incubation time is 72～200 hours；
3) results of mixotroph heterotrophic cell: the heterotrophic cell of take the logarithm Later growth or stable phase, harvested by centrifugation under the slow-speed of revolution of 2000～8000r/min, the mixotroph heterotrophic cell of results is with aseptic water washing twice, and then results；
4) it is transferred to high ammonia-nitrogen wastewater and carries out autotrophy cultivation, absorb ammonia nitrogen in high density and purify waste water: the mixotroph heterotrophic cell of results being added high ammonia-nitrogen wastewater carries out autotrophy cultivation, the volume ratio of mixotroph heterotrophic cell and high ammonia-nitrogen wastewater is 1:1～1:100, nutrition conversion process needs absorb substantial amounts of high ammonia nitrogen nutrient for synthesizing chloroplast associated component and the various enzymes relevant with photosynthesis, thus reaching to purify the purpose of waste water；
Described autotrophy condition of culture is as follows: temperature is 20～45 DEG C；Illumination cultivation nitrogenous source is ammonia nitrogen, by adding different amounts of NH4Cl regulates ammonia nitrogen initial concentration in high ammonia-nitrogen wastewater, makes ammonia nitrogen initial concentration between 40mg/L～160mg/L, wherein NH4The concentration of Cl is 40mg/L, 80mg/L and 160mg/L；Pass into air or air and CO2Mixing gas, ventilation 50～300L/h；CO2Concentration 0.1～15%；Adopting the sun exposure of 10～200 μm of ol/m2/s in incubation, pH value controls 5～9, and total incubation time is 4～400 hours；
Described high ammonia-nitrogen wastewater formula is as follows: NaCl0.007g/L, MgSO4·7H2O0.002g/L、CaCl2·2H2O0.004g/L、KH2PO40.0085g/L、K2HPO40.0217g/L、Na2HPO40.025g/L, Trace Metal solution 0.1ml/L, wherein Trace Metal solution composition: H3BO35.7g/L、MnCl2·4H2O2.5g/L、ZnSO4·7H2O11g/L、FeSO4·7H2O2.5g/L、Na2MoO4·2H2O0.15g/L、Na2EDTA25g/L and CoCl2·6H2O0.8g/L。
2. mixotroph nutrition according to claim 1 converts the method processed for high ammonia-nitrogen wastewater, it is characterised in that: described autotrophy, raise together with or heterotrophism used medium formula is: K2HPO4·3H2O0.04g/L、MgSO4·7H2O0.075g/L、CaCl2·2H2O0.036g/L, citric acid 0.006g/L, ferric ammonium citrate 0.006g/L, EDTA0.001g/L, NaNO31.5g/L、Na2CO30.02g/L and A5 liquid microelement 1.5ml/L；Wherein, A5 liquid microelement composition: H3BO32.86g/L、MnCl2·4H2O1.81g/L、ZnSO4·7H2O0.222g/L、NaMoO4·2H2O0.39g/L、CuSO4·5H2O0.079g/L and CoCl2·6H2O0.05g/L。
3. mixotroph nutrition according to claim 1 converts the method processed for high ammonia-nitrogen wastewater, it is characterised in that: step 1) in, described mixotroph is preferably microalgae or photosynthetic bacteria.
4. mixotroph nutrition according to claim 1 converts the method processed for high ammonia-nitrogen wastewater, it is characterized in that: step 2) in, described organic carbon source is preferably glucose, fructose, corn starch hydrolysate, tapioca hydrolysate, molasses, ribose, deoxyribose, ribulose, acetic acid, propanoic acid, butanoic acid or glycerol.
5. mixotroph nutrition according to claim 3 converts the method processed for high ammonia-nitrogen wastewater, it is characterised in that: step 2) in, described organic carbon source initial reduction sugar concentration is 1～20g/L.
6. mixotroph nutrition according to claim 1 converts the method processed for high ammonia-nitrogen wastewater, it is characterized in that: step 2) in, described it is preferably sanitary wastewater, waste water of paper mill, molasses containing waste water, beer brewery water, dairy food processing plants waste water, biogas fermentation waste water or animal wastes waste water rich in organic carbon waste water.
7. mixotroph nutrition according to claim 2 converts the method processed for high ammonia-nitrogen wastewater, it is characterised in that: described microalgae is preferably Chlorella, barrel mast Trentepohlia, diatom, rhombus algae, splits kettle algae, Dunaliella, scenedesmus, Nannochloropsis oceanica, Chlamydomonas, flat algae or empty ball Trentepohlia.
8. mixotroph nutrition according to claim 2 converts the method processed for high ammonia-nitrogen wastewater, it is characterized in that: described photosynthetic bacteria is selected from cyanobacteria or purple bacteria, described cyanobacteria is selected from Synechococcus genus or the blue Pseudomonas that quivers greatly, and described purple bacteria is selected from Rhodospirillum, Rhodopseudomonas or Rhodomicrobium.
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