CN103396950B - A kind of natural pond liquid ecological purification method based on both culturing microalgae - Google Patents

A kind of natural pond liquid ecological purification method based on both culturing microalgae Download PDF

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CN103396950B
CN103396950B CN201310347109.8A CN201310347109A CN103396950B CN 103396950 B CN103396950 B CN 103396950B CN 201310347109 A CN201310347109 A CN 201310347109A CN 103396950 B CN103396950 B CN 103396950B
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natural pond
liquid
algae
pond liquid
chlorella
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CN103396950A (en
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孙利芹
邹强
左志鹏
刘红辉
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烟台大学
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    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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Abstract

The invention discloses a kind of natural pond liquid ecological purification method based on both culturing microalgae, comprise the steps: the liquid pre-treatment of (1) natural pond; (2) domestication of the micro-algae of autotrophic type is cultivated, and obtains the algae strain that can grow fast in the liquid of 70%-100% natural pond; (3) preparation of seed liquor; (3) micro algae growth coupling natural pond liquid purifying method: the algae kind after domestication is inoculated in Race-way photobioreactor after spreading cultivation cultivates, adopt the Method means that Semi-continuous cultivation method and feed supplement feeding culture etc. are optimized, obtain the chlorella biomass of high-density growth, purify natural pond liquid simultaneously; (4) the biochemical broken wall of microalgae cell: microalgae cell is introduced biochemical broken wall pond, throws in fresh-water fishes, obtains the algae slurry of broken wall; (5) microalgae cell recycle natural pond liquid.Described method is not only natural pond liquid purification and provides method, and the frustule of results and natural pond liquid can be recycled, and achieve the ecology process of natural pond liquid, while environmental protect, produce economic and social benefit.

Description

A kind of natural pond liquid ecological purification method based on both culturing microalgae
Technical field
The present invention relates to the purifying method of a kind of natural pond liquid, particularly relate to a kind of natural pond liquid ecological purification method based on both culturing microalgae.
Background technology
Natural pond liquid, be otherwise known as " anaerobic fermented liquid ", and being that animal excrement, stalk etc. are organic residually after anaerobically fermenting has water miscible by product.Along with agricultural sector structure adjustment and new countryside construction, intensive livestock and poultry breeding industry fast development, as the product natural pond liquid after biogas anaerobic fermentation, the annual generation of current China is about more than 200,000,000 ton, it is fertile or directly discharge that these waste liquids are used as agriculture, seldom considers that whether and safety for agricultural use emission compliance.In fact, after anaerobic digestion, natural pond liquid is still containing a large amount of nitrogen, phosphorus and metal, is regarded as high-concentration sewage.For natural pond, pig farm liquid, its COD is still at 2000 ~ 4000mg/L, and total nitrogen is at 317 ~ 423mg/L, and total phosphorus is at 34.7 ~ 52.4mg/L, and total potassium is at 225 ~ 350mg/L, and also have the heavy metal such as copper and zinc in addition, direct discharge can cause body eutrophication.Along with the attention of Environmental protection, the strict control of sewage drainage standard, livestock and poultry farm discharged wastewater met the national standard and utilization have become the important factor affecting plant's survival and development.
Method for the treatment of city waste water is continued to use in the purifying method research of natural pond liquid more, adopt the methods such as physics, chemistry, biology, as the natural ecological resource methods such as biological pond method and artificial swamp and the industrial treatment pattern adopting the reactors such as MBR, SBR, reverse osmosis, but these methods are due to features such as the too high and operative technique difficulty of running cost are large at present, be difficult to promote raiser, and aforesaid method is not fairly obvious to N, P removal effect in water, therefore, natural pond liquid directly discharges and remains processing mode main at present after simple Air Exposure.Also some are had natural pond liquid to be used for field irrigation, natural pond immersion kind, foliage-spray, soilless culture etc.; but due to transportation cost and use security problems; make the usage quantity of natural pond liquid little; a large amount of natural ponds liquid that large-scale cultivation industry of cannot dissolving anaerobically fermenting produces, therefore directly discharge is still the main processing ways of current natural pond liquid.
Summary of the invention
The object of this invention is to provide a kind of natural pond liquid ecological purification method based on both culturing microalgae, solve the unprocessed direct exhaust emission environment of a large amount of natural ponds liquid that current livestock and poultry cultivation produces, employing conventional sewage treatment process cost is high, operation is difficult, denitrogenation, phosphor-removing effect are poor, are difficult to the common problem promoted.
Based on a natural pond liquid ecological purification method for both culturing microalgae, comprise the steps:
(1) natural pond liquid pre-treatment: the natural pond liquid after anaerobically fermenting is after solid-liquid separation, aerobic treatment, and enter sterilization pool sterilization successively, equalizing tank carries out potential of hydrogen and nutritive ingredient adjustment, the water of equalizing tank is introduced seed and spread cultivation in pond and open optical-biological reaction pond;
(2) domestication of the micro-algae of autotrophic type is cultivated: within the scope of concentration 30-100%, using the natural pond liquid that concentration raises successively as nutrient solution, cultivates micro-algae, obtain the algae strain that can grow fast in the liquid of 70%-100% natural pond according to the method domestication of microbial acclimation;
(3) preparation of seed liquor: the chlorella algae strain after domestication is inoculated into open seed and spreads cultivation in pond and cultivate 3-5 days, obtain the chlorella seed liquor being in logarithmic phase, i.e. algae kind;
(4) the incubation growth coupling natural pond liquid purification of micro-algae: the algae kind being in logarithmic phase after spreading cultivation is inoculated in Race-way photobioreactor cultivates, makes natural pond liquid reach secondary waste water emission standard while acquisition high-density chlorella biomass;
(5) the biochemical broken wall of microalgae cell: the microalgae cell cultivated in the liquid of natural pond 7-9 days is introduced biochemical broken wall pond, the fresh-water fishes dropping into some amount and population cultivate, and carry out broken wall treatment to chlorella;
(6) the gathering of microalgae cell: the frustule after adopting flocculating settling method to collect broken wall, obtains chlorella algae slurry, can be used for the production of high-protein feed additive; Top clear liquor be collected in pond can be used for breeding fish as reuse water, the purposes such as irrigation.
Natural pond liquid ecological purification method based on both culturing microalgae of the present invention, wherein step (1) is specially: the natural pond liquid after anaerobically fermenting enters aeration tank and carries out Air Exposure after solid-liquid separating machine is separated, natural pond liquid after aeration enters sterilization pool, be that 8-10% chlorine bleach liquor carries out disinfection by concentration, addition is 1-3mL/L, thereafter neutralize with the Sulfothiorine of same concentrations, described clorox stoste effective chlorine content % >=10.0;
The adjustment of described nutritive ingredient refers to the supplementary adjustment of lettuce, potential of hydrogen and nutritive salt composition; Described aeration process adds the unslaked lime of water body volume 0.3-0.5 ‰, after continuous aeration 24h, and interval 3 hours continuous aeration 24h again, precipitation 2h.
Natural pond liquid ecological purification method based on both culturing microalgae of the present invention, wherein step (2) is specially: chlorella is placed in illumination box, be tame in the natural pond liquid of 30%, 50%, 70%, 80% and 100% successively in concentration, culture temperature is 23-25 DEG C, pH6.5-7.5, illumination 3500-4000Lx; From the liquid of lower concentration natural pond, the well-grown algae strain of picking is inoculated in the natural pond liquid of greater concn, finally tames, filters out the algae strain that can grow fast in the liquid of 70-100% natural pond respectively.
Natural pond liquid ecological purification method based on both culturing microalgae of the present invention, wherein step (3) is specially: the chlorella algae strain after domestication is inoculated into 20m 3open seed spreads cultivation in pond and cultivates 3-5 days, nutrient solution is that natural pond liquid and fresh water mix according to 5:5 ratio, according to SE formulated nutritive salt, according to the chlorella algae kind after the inoculum size access domestication of 3-5%, its Initial seeding density is made to reach 1000-1500 ten thousand cells/ml, concrete culture condition is pH6.8-7.5, culture temperature 23-25 DEG C, intensity of illumination 3500-4000Lx, cultivated days is 3-5d, be as the criterion vegetative period to reach cell log, obtain the chlorella seed liquor being in logarithmic phase.
Natural pond liquid ecological purification method based on both culturing microalgae of the present invention, wherein step (4) is specially: the algae kind after spreading cultivation is inoculated into 100m 3open optical-biological reaction pond in, inoculum size is 10-15%, and inoculum density is 0.8-1.0 × 10 8individual cells/ml, nutrient solution select respectively concentration be 70%, 80% and 100% natural pond liquid;
Wherein, microdisk electrode process adopts feed supplement fed-batch mode and Semi-continuous cultivation method: according to micro algae growth curve, one time of nutrition liquid is added with reference to SE formula, wherein TN and the TP concentration that detects according to real-time online of the situation of adding of nitrogen salt and microcosmic salt and determining at 4-5 days; Cultural method adopts Semi-continuous cultivation: according to the change in concentration rule of COD, TN, TP in the liquid of natural pond, selects to walk the micro algae culturing liquid of 30%-50% at 5-7 days pumps, and supplements the fresh natural pond liquid of equivalent, after this upgrades every 3-4 days and replaces once.
Natural pond liquid ecological purification method based on both culturing microalgae of the present invention, the culture temperature of wherein said chlorella is 22-26 DEG C, and illumination 5000-8000Lx, light application time 16h/d, pass into air speed 8-10vvm.
Natural pond liquid ecological purification method based on both culturing microalgae of the present invention, wherein step (5) is specially: the chlorella algae liquid cultivated in the liquid of natural pond 7-9 days is introduced biochemical broken wall pond, adds fresh-water fishes, and cultivation density is 1-3 bar/m 3, remove fish after 3 days, add flocculation agent precipitation frustule, collect the algae slurry bottom pond.
Natural pond liquid ecological purification method based on both culturing microalgae of the present invention, wherein said fresh-water fishes are silver carp, carp and crucian, and described flocculation agent is the alum of 200-300mg/mL, and during sedimentation, pH value is 9-11.
Natural pond liquid ecological purification method difference from prior art based on both culturing microalgae of the present invention is:
(1) be employed herein micro-algae, micro-algae is a class photo-autotroph, has that photosynthetic efficiency is high, the feature of fast growth, can the effectively body eutrophication such as absorbed nitrogen, phosphorus composition and heavy metal in its production process, reaches the object of purification natural pond liquid.The micro algae biomass of results can processing and utilization further, and the natural pond liquid after purification may be used for irrigating, rinsing colony house etc., realizes resource and energy regeneration and the recycling of natural pond liquid, while environmental protect, and increase economic and social benefit.
(2) the invention solves the unprocessed direct exhaust emission environment of a large amount of natural ponds liquid that current livestock and poultry cultivation produces, adopt that conventional sewage treatment process cost is high, operation is difficult, denitrogenation, phosphor-removing effect are poor, are difficult to the common problem of popularization; By providing a kind of method utilizing microdisk electrode process to reduce Zhao Yezhong contamination index, it is made to reach emission standard, described method is not only for a large amount of natural pond liquid purifications provides method, and results frustule can deeply add further and purify after natural pond liquid can recycle, achieve the ecology process of natural pond liquid, while environmental protect, produce economic and social benefit.
(3) traditional physics, chemistry and closed bioreactor sewage water processing mode, there is complicated operation, running cost is high, the shortcoming that should not promote, and the present invention adopts open pond both culturing microalgae method to purify waste water, and cost is low, simple to operate, very easily promotes.
(4) utilize the feature of autotrophic type micro algae growth process energy nitrogen use efficient phosphorus to dispose of sewage in the present invention, effectively can remove TN and TP in sewage, reduce COD, overcome the shortcoming to the poor removal effect of nitrogen phosphorus in water in general sewage water treatment method.
(5) natural pond liquid is adopted to cultivate micro-algae, the object of purification of water quality is reached while harvesting microalgae biomass, the microalgae cell of results can continue to serve as the processing etc. of feed, produce certain economic benefit, and natural pond liquid after purification can reuse be used for breeding fish, irrigation etc., realize ecology purification of water quality.
The present invention is based on main improvement in the natural pond liquid ecological purification method of both culturing microalgae to be: first being purified with natural pond liquid by microdisk electrode and combine, is secondly adopt feed supplement stream to add and Semi-continuous cultivation method in microdisk electrode and natural pond liquid scavenging process; In the method for existing report, the general Closed photobioreactor that adopts cultivates micro-algae, reactor complicated operation, toxigenic capacity is high, not easily promotes, and it focuses on on the liquid cultivating microalgae of natural pond, and the present invention adopts open pond reactor, equipment cost and running cost are all low, simple to operate, easily promote raiser, special emphasis is in the purifying treatment to natural pond liquid, enables natural pond liquid reach emission standard, and harvesting microalgae does its use simultaneously.
In addition, aeration process in the method for the invention adds unslaked lime, can play to assist and kill protozoon, particle diameter can be removed comparatively large, the material that biodegradability is not strong, interval 3 hours continuous aeration 24h again after continuous aeration 24h simultaneously, different aeration mode produces different results, adopt aeration mode of the present invention more to contribute to the breeding of aerobic bacteria, reduce the conversion of water body COD and organonitrogen phosphorus compound, to improve in follow-up workshop section micro-algae to the decontamination effect improving of water body; The present invention adds fresh-water fishes and carries out biochemical broken wall in biochemical broken wall pond, replaces physics, the chemical processes such as traditional ultrasonic wave, high-pressure homogenization or enzyme process, simple to operate, cost is low, effective.
Below in conjunction with accompanying drawing, the natural pond liquid ecological purification method based on both culturing microalgae of the present invention is described further.
Accompanying drawing explanation
Fig. 1 is the schematic flow sheet of the natural pond liquid ecological purification method based on both culturing microalgae of the present invention;
Fig. 2 is the growth figure of chlorella after domestication in the liquid of different concns natural pond of the embodiment of the present invention.
Embodiment
Embodiment
As Fig. 1, the natural pond liquid ecological purification method that the present invention is based on both culturing microalgae comprises the steps:
1, natural pond liquid pre-treatment: the natural pond liquid after anaerobically fermenting is through Air Exposure, and the natural pond liquid after aeration enters sterilization pool after solid-liquid separating machine is separated, and sterilizes with the clorox 1-3mL/L that concentration is 8%-10%, the Sulfothiorine neutralization of same concentrations; In equalizing tank, carry out the adjustment of nutritive ingredient, refer to the supplementary adjustment of lettuce, potential of hydrogen and nutritive salt composition; The water of described equalizing tank is incorporated into seed and spreads cultivation pond and open optical-biological reaction pond.
Described aeration process adds the unslaked lime of 0.3-0.5 ‰, interval 3 hours continuous aeration 24h again after continuous aeration 24h; Described regulating measures and microdisk electrode matching criteria.
2, the domestication of the micro-algae of autotrophic type is cultivated: with photoautotrophy type Chlorella vulgaris (Chlorellamulgaris, purchased from Inst. of Hydrobiology, Chinese Academy of Sciences's algae kind storehouse FACHB) for algae kind is within the scope of concentration 30-100%, using the natural pond liquid that concentration raises successively as nutrient solution, cultivate micro-algae according to the method domestication of microbial acclimation, obtain the algae strain that can grow fast in the liquid of 70%-100% natural pond; Different concns natural pond liquid domestication chlorella growth situation as shown in Figure 2.
Culture medium prescription is optimized, and according to the content of total nitrogen (TN) and total phosphorus (TP) in the liquid of initial natural pond, finely tunes, make NaNO in nutrient solution with reference to SE formula 3: 5-15g/100mL, K 2hPO4:3.0-7.5g/100mL, KH 2pO4:10-17.5g/100mL, other is constant.
SE original formulation following (formula source: Inst. of Hydrobiology, Chinese Academy of Sciences's algae kind storehouse):
(1) NaNO 3: 25g/100mL), K 2hPO4:7.5g/100mL, MgSO 47H2O:7.5g/100mL, CaCl 22H 2o:2.5g/100mL, KH 2pO4:17.5g/100mL, NaCl:2.5g/100mL, FeCl 3.6H 2o:0.05g/100mL, above inorganic salt addition is 1mL/L.
(2) EDTA-Fe1mL/L, compound method is as follows: 1. 1NHCl: get 4.1ml concentrated hydrochloric acid distilled water diluting to 50ml; 2. 0.1NEDTA-Na takes 0.9306g and is dissolved in 50ml distilled water; 3. FeCl is taken 3.6H 2o0.901g is dissolved in the 1NHCl that more than 10ml step prepared, and the 0.1NEDTA-Na then prepared with 10ml mixes, and adds distilled water diluting to 1000ml.
(3) micro-1ml/L, fills a prescription as follows: H 3bO 32.86g/LdH 2o, MnCl 24H 2o1.86g/L, ZnSO 47H 2o0.22g/L, Na 2moO 4.2H 2o0.39g/L, CuSO 4.5H 2o0.08g/L, Co (NO 3) 2.6H 2o0.05g/L.
(4) soil extract 40mL/L, preparation method is as follows: get garden soil and do not execute overfertilization 200g and be placed in beaker or triangular flask, add distilled water 1000 milliliters, bottleneck porous plug seals, and boiling water heats 3 hours in a water bath, cooling, precipitate 24 hours, this process carries out 3 times continuously, then filters, get supernatant liquor, save backup in 4 DEG C of refrigerators after sterilizing in high-pressure sterilizing pot.
3, the preparation (spreading cultivation) of seed liquor: the chlorella algae strain after domestication is inoculated into 20m 3open seed spreads cultivation in pond and cultivates 3-5 days, nutrient solution is that natural pond liquid and fresh water mix according to 5:5 ratio, according to SE formulated nutritive salt, according to the chlorella algae kind after the inoculum size access domestication of 3-5%, its Initial seeding density is made to reach 1000-1500 ten thousand cells/ml, concrete culture condition is pH6.8-7.5, culture temperature 23-25 DEG C, intensity of illumination 3500-4000Lx, cultivated days is 3-5d, be as the criterion vegetative period to reach cell log, obtain the chlorella seed liquor being in logarithmic phase, i.e. algae kind.
4, the incubation growth coupling natural pond liquid purification of micro-algae: the algae kind after spreading cultivation is inoculated into 100m 3open optical-biological reaction pond in, control microdisk electrode condition and cultural method, make the content of total nitrogen (TN) in the liquid of natural pond, total phosphorus (TP), COD and poisonous metal reach " livestock and poultry breeding industry pollutant emission standard " (GB18596-2001) or town sewage secondary discharge standard.Concrete operation step is as follows:
(1) be in the domestication chlorella algae strain of logarithmic phase according to the inoculum size access of 10-15%, inoculation initial density is reached for 0.8-1.0 × 10 8individual cells/ml.
(2) microdisk electrode process adopts feed supplement fed-batch mode, and lettuce originally selects 70%, 80% and 100% respectively.According to micro algae growth curve, added one time of nutrition liquid at 4-5 days with reference to SE formula, but TN and the TP concentration that detects according to real-time online of the situation of adding of nitrogen salt and microcosmic salt and determining.
(2) cultural method adopts Semi-continuous cultivation method, according to the concentration of COD, TN, TP in the liquid of natural pond, select the fresh natural pond liquid adopted at 5-7 days to upgrade the micro algae culturing liquid substituting 30%-50%, after this every 3-4 days, the method optimal way of carrying out once carried out the purifying treatment of natural pond liquid.
In above-mentioned steps, the culture temperature of chlorella is 22-26 DEG C, and illumination 5000-8000Lx, light application time 16h/d, pass into air speed 8-10vvm.Natural pond liquid is after chlorella growth cycle purifying treatment, find that 70% natural pond liquid can reach best decontamination effect improving, as shown in table 1, final each pollution index value is total nitrogen 199.77mg/L, total phosphorus 2.14mg/L, ammonia nitrogen 1.59mg/L, active phosphorus 0.89mg/L, COD283.2mg/L, each index all reaches national town sewage secondary discharge standard.
Contamination index's contrast before and after the liquid process of table 1 natural pond
5, the biochemical broken wall of microalgae cell: the microalgae cell cultivated in the liquid of natural pond 7-9 days is introduced biochemical broken wall pond, cultivates fresh water fishes wherein, add silver carp, carp and crucian, cultivation density is 1-3 bar/m 3, remove fish after 3 days, carry out biochemical broken wall.
6, the gathering of microalgae cell: adopt flocculation agent sedimentation method precipitation frustule, described flocculation agent adopts the alum of 200-300mg/mL, and during sedimentation, pH adjusts to 9-11; In 2h, the rate of descent of chlorella cells can reach more than 85%.Collect the algae slurry bottom pond, can be used for the production of high-protein feed additive; Top clear liquor is collected in the purposes such as middle pond can be used for breeding fish as reuse water, irrigation.
Above-described embodiment is only be described the preferred embodiment of the present invention; not scope of the present invention is limited; under not departing from the present invention and designing the prerequisite of spirit; the various distortion that those of ordinary skill in the art make technical scheme of the present invention and improvement, all should fall in protection domain that claims of the present invention determines.

Claims (8)

1., based on a natural pond liquid ecological purification method for both culturing microalgae, it is characterized in that: comprise the steps:
(1) natural pond liquid pre-treatment: the natural pond liquid after anaerobically fermenting, after solid-liquid separation, aerobic treatment, enters sterilization pool sterilization successively, equalizing tank carries out nutritive ingredient adjustment, the water of equalizing tank is introduced seed and is spread cultivation in pond and open optical-biological reaction pond;
(2) domestication of the micro-algae of autotrophic type is cultivated: within the scope of concentration 30-100%, using the natural pond liquid that concentration raises successively as nutrient solution, cultivates micro-algae, obtain the algae strain that can grow fast in the liquid of 70%-100% natural pond according to the method domestication of microbial acclimation;
(3) preparation of seed liquor: the chlorella algae strain after domestication is inoculated into open seed and spreads cultivation in pond and cultivate 3-5 days, obtain the chlorella seed liquor being in logarithmic phase, i.e. algae kind;
(4) the incubation growth coupling natural pond liquid purification of micro-algae: the algae kind being in logarithmic phase after spreading cultivation is inoculated in open optical-biological reaction pond cultivates, makes natural pond liquid reach secondary waste water emission standard while acquisition high-density chlorella biomass;
(5) the biochemical broken wall of microalgae cell: the microalgae cell cultivated in the liquid of natural pond 7-9 days is introduced biochemical broken wall pond, the fresh-water fishes dropping into some amount and population cultivate, and carry out broken wall treatment to chlorella;
(6) the gathering of microalgae cell: the frustule after adopting flocculating settling method to collect broken wall, obtains chlorella algae slurry, for the production of high-protein feed additive; Top clear liquor to be collected in pond as reuse water for being breeded fish, irrigation.
2. the natural pond liquid ecological purification method based on both culturing microalgae according to claim 1, it is characterized in that: step (1) is specially: the natural pond liquid after anaerobically fermenting enters aeration tank and carries out Air Exposure after solid-liquid separating machine is separated, natural pond liquid after aeration enters sterilization pool, be that 8-10% chlorine bleach liquor carries out disinfection by concentration, addition is 1-3mL/L, thereafter neutralize with the Sulfothiorine of same concentrations, described chlorine bleach liquor's effective chlorine content >=10%;
Described nutritive ingredient adjustment refers to the supplementary adjustment of lettuce, potential of hydrogen and nutritive salt composition; Aeration process adds the unslaked lime of water body volume 0.3-0.5 ‰, after continuous aeration 24h, and interval 3 hours continuous aeration 24h again, precipitation 2h.
3. the natural pond liquid ecological purification method based on both culturing microalgae according to claim 2, it is characterized in that: step (2) is specially: chlorella is placed in illumination box, be tame in the natural pond liquid of 30%, 50%, 70%, 80% and 100% successively in concentration, culture temperature is 23-25 DEG C, pH6.5-7.5, illumination 3500-4000Lx; From the liquid of lower concentration natural pond, the well-grown algae strain of picking is inoculated in the natural pond liquid of greater concn, finally tames, filters out the algae strain that can grow fast in the liquid of 70-100% natural pond respectively.
4. the natural pond liquid ecological purification method based on both culturing microalgae according to claim 3, is characterized in that: step (3) is specially: the chlorella algae strain after domestication is inoculated into 20m 3open seed spreads cultivation in pond and cultivates 3-5 days, nutrient solution is that natural pond liquid and fresh water mix according to 5:5 ratio, according to SE formulated nutritive salt, according to the chlorella algae kind after the inoculum size access domestication of 3-5%, its Initial seeding density is made to reach 1000-1500 ten thousand cells/ml, culture condition is pH value 6.8-7.5, culture temperature 23-25 DEG C, intensity of illumination 3500-4000Lx, cultivated days is 3-5d, be as the criterion vegetative period to reach cell log, obtain the chlorella seed liquor being in logarithmic phase.
5. the natural pond liquid ecological purification method based on both culturing microalgae according to claim 1 or 2 or 3 or 4, is characterized in that: step (4) is specially: the algae kind after spreading cultivation is inoculated into 100m 3open optical-biological reaction pond in, inoculum size is 10-15%, and inoculum density is 0.8-1.0 × 10 8individual cells/ml, nutrient solution select respectively concentration be 70%, 80% and 100% natural pond liquid;
Wherein, microdisk electrode process adopts feed supplement fed-batch mode and Semi-continuous cultivation method: according to micro algae growth curve, one time of nutrition liquid is added with reference to SE formula, wherein TN and the TP concentration that detects according to real-time online of the situation of adding of nitrogen salt and microcosmic salt and determining at 4-5 days; Cultural method adopts Semi-continuous cultivation: according to the change in concentration rule of COD, TN, TP in the liquid of natural pond, selects to walk the micro algae culturing liquid of 30%-50% at 5-7 days pumps, and supplements the fresh natural pond liquid of equivalent, after this upgrades every 3-4 days and replaces once.
6. the natural pond liquid ecological purification method based on both culturing microalgae according to claim 5, is characterized in that: the culture temperature of described chlorella is 22-26 DEG C, and illumination 5000-8000Lx, light application time 16h/d, pass into air speed 8-10vvm.
7. the natural pond liquid ecological purification method based on both culturing microalgae according to claim 5, it is characterized in that: step (5) is specially: the chlorella algae liquid cultivated in the liquid of natural pond 7-9 days is introduced biochemical broken wall pond, add fresh-water fishes, cultivation density is 1-3 bar/m 3, remove fish after 3 days, add flocculation agent sedimentation frustule, collect the algae slurry bottom pond.
8. the natural pond liquid ecological purification method based on both culturing microalgae according to claim 7, it is characterized in that: described fresh-water fishes are silver carp, carp and crucian, and described flocculation agent is the alum of 200-300mg/mL, during sedimentation, pH value is 9-11.
CN201310347109.8A 2013-08-09 2013-08-09 A kind of natural pond liquid ecological purification method based on both culturing microalgae CN103396950B (en)

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