CN101824385A - Method for cultivating chlorella with methane waste liquor - Google Patents

Abstract

The invention discloses a method for cultivating chlorella with methane waste liquor, comprising the following steps: preparing the methane waste liquor into mixed culturing liquid, cultivating pyrenoid chlorella, etc. In the invention, methane waste liquor is used as main material of culturing liquid, thereby remarkably reducing cultivation cost, purifying methane waste liquor, obtaining pyrenoid chlorella with high economic value, and having better economic and social benefits.

Description

Utilize methane waste liquor to cultivate the method for chlorella

Technical field

The present invention relates to utilize methane waste liquor to cultivate the method for chlorella, relate in particular to a kind of method of utilizing methane waste liquor to cultivate chlorella.

Background technology

In the biogas waste water, nutritive element such as nitrogen, phosphorus and organic content are abundant, exceeding standard and excessively discharging of it is an important factor that causes body eutrophication and peripheral water environment to pollute, although developed many treatment technology and equipment at present at biogas waste water, but because correlation technique is improved and ripe and higher running cost and energy consumption not enough, practical application effect is unsatisfactory.

Chlorella pyrenoidesa is a unicellular algae, is distributed widely in the whole world, is a kind of important bait of aquatic living things.Simultaneously, the content of its protein, fat and carbohydrate is very high, and multivitamin is arranged again, thereby can be used as the part of feed.Studies have shown that the glycoprotein in the chlorella has the significant activity that presses down the anti-knurl of knurl, enhancing immunity and anti-virus infection.Therefore, cultivate the exploitation chlorella and have higher economic worth.At present laboratory and producing is gone up the abundant f/2 nutrient solution of element such as main nitrogenous, the phosphorus that adopts artificial preparation and is cultivated.Because nutritive element and trace element are numerous in the nutrient solution, not only preparation trouble has also increased the cultivation cost.

Discover that little algae such as chlorella can absorb, consume nutritive element and organic matters such as nitrogen in the water body, phosphorus, and can absorb water body and carbon dioxide in air is carried out photosynthesis.Therefore, utilize methane waste liquor to cultivate chlorella, can not only purifying marsh gas waste water, reduce carbon emission, and the chlorella of results also can be used for the exploitation of bait, feed and the medicine of aquatic animal, this both reduced biogas waste water processing costs, reduced environmental pollution, also the production for economic little algaes such as chlorellas provides a development approach cheaply, can play the effect that kills two birds with one stone.

Summary of the invention

The object of the present invention is to provide the lower methane waste liquor that utilizes of a kind of cost of development to cultivate the method for chlorella.

For achieving the above object, technical scheme of the present invention is as follows:

The present invention is a kind of method of utilizing methane waste liquor to cultivate chlorella, and it may further comprise the steps:

(1) the methane waste liquor filtration is made into mixed-culture medium: in methane waste liquor, add the material of following content,

SODIUMNITRATE 0-50mg/L;

Potassium primary phosphate 0-3mg/L;

Water glass 0-6mg/L

Ironic citrate 0-2mg/L;

(2) cultivate Chlorella pyrenoidesa: with the mixed-culture medium produced under 0-35 ℃ of temperature, pH3-7, illumination 500LX-20000LX, the initial inoculation density of Chlorella pyrenoidesa is 0.002-5mg/mL, cultivates can obtain a large amount of Chlorella pyrenoidesas in 5-30 days.

Compare with existing culture technique, outstanding advantage of the present invention is to have adopted the nutrient solution based on methane waste liquor, thereby has reduced the cultivation cost significantly, and when obtaining this little algae, realizes the purification to methane waste liquor.

Method in the chief editor who spreads out according to thunder " cultivation water environment chemical experiment ", to the nitrogen in the methane waste liquor, indexs such as phosphorus are measured (nitric nitrogen employing zinc-cadmium reduction method, ammonia-state nitrogen adopts the indophenols blue laws, nitrite nitrogen adopts diazonium-azo light-intensity method, active phosphorus adopts molybdenum-antimony-anti-method), the result shows, growth (as shown in Figure 1) along with Chlorella pyrenoidesa, nitric nitrogen in the water body, nitrite nitrogen, the content of nutritive elements such as active phosphorus declines to a great extent (as shown in Figure 2), cultivation through 11 days, Chlorella pyrenoidesa reaches 75.2% to the clearance rate of active phosphorus, clearance rate to nitric nitrogen is 52.8%, clearance rate to nitrite nitrogen is 84.1%, and the 3rd day of ammonia-state nitrogen is just removed fully, thereby has purified methane waste liquor preferably.

The present invention utilizes methane waste liquor to cultivate Chlorella pyrenoidesa, in purifying marsh gas waste water, has also obtained to have the Chlorella pyrenoidesa of higher economic worth, has better economic and social benefit.

The present invention is further illustrated below in conjunction with the drawings and specific embodiments.

Description of drawings

Fig. 1 is the growth curve chart of Chlorella pyrenoidesa of the present invention in methane waste liquor;

Fig. 2 is the purification graphic representation of Chlorella pyrenoidesa of the present invention to methane waste liquor.

Embodiment

Embodiment 1

Get the methane waste liquor 1L after the filtration, add SODIUMNITRATE 25mg, potassium primary phosphate 1.5mg, water glass 3mg, ironic citrate 1mg adds Chlorella pyrenoidesa algae kind 10mg again, under 20 ℃ of temperature, pH5, illumination 05000LX cultivate down can obtain a large amount of Chlorella pyrenoidesas in 10 days.

Embodiment 2

Get the methane waste liquor 1L after the filtration, add SODIUMNITRATE 0mg, potassium primary phosphate 0mg, water glass 0mg, ironic citrate 0mg adds Chlorella pyrenoidesa algae kind 2mg again, and under 0 ℃ of temperature, pH3, illumination 5000LX cultivate down can obtain a large amount of Chlorella pyrenoidesas in 5 days.

Embodiment 3

Get the methane waste liquor 1L after the filtration, add SODIUMNITRATE 50mg, potassium primary phosphate 3mg, water glass 6mg, ironic citrate 2mg adds Chlorella pyrenoidesa algae kind 5g again, under 35 ℃ of temperature, pH7, illumination 20000LX cultivate down can obtain a large amount of Chlorella pyrenoidesas in 30 days.

Embodiment 4

Get the methane waste liquor 1L after the filtration, add SODIUMNITRATE 30mg, potassium primary phosphate 2mg, water glass 4mg, ironic citrate 0.8mg adds Chlorella pyrenoidesa algae kind 4g again, under 25 ℃ of temperature, pH6, illumination 4000LX cultivate down can obtain a large amount of Chlorella pyrenoidesas in 15 days.

Claims (1)

1. method of utilizing methane waste liquor to cultivate chlorella, it is characterized in that: it may further comprise the steps:

(1) the methane waste liquor filtration is made into mixed-culture medium: in methane waste liquor, add the material of following content,

SODIUMNITRATE 0-50mg/L;

Potassium primary phosphate 0-3mg/L;

Water glass 0-6mg/L

Ironic citrate 0-2mg/L;

(2) cultivate Chlorella pyrenoidesa: with the mixed-culture medium produced under 0-35 ℃ of temperature, pH3-7, illumination 500LX-20000LX, the initial inoculation density of Chlorella pyrenoidesa is 0.002-5mg/mL, cultivates can obtain a large amount of Chlorella pyrenoidesas in 5-30 days.

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