CN103396950A - Biogas slurry ecological purification method based on microalgae cultivation - Google Patents
Biogas slurry ecological purification method based on microalgae cultivation Download PDFInfo
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Abstract
The invention discloses a biogas slurry ecological purification method based on microalgae cultivation. The method comprises the following steps: (1) biogas slurry pretreatment; (2) habituated culture of autotrophic microalgae, namely, obtaining algae strains capable of rapidly growing in 70%-100% biogas slurry; (3) preparation of a seed solution; (4) a biogas slurry purification method during growing of microalgae, namely, after expanded cultivation, inoculating the habituated algae species into an open photobioreactor for cultivation, obtaining chlorella biomass growing by high density through a semi-continuous culture method and optimized methods such as fed-batch cultivation, and purifying the biogas slurry; (5) biochemical breaking of microalgae cell walls, namely, introducing the microalgae cells into a biochemical wall breaking pool, putting freshwater fish into the pool, and obtaining algae slurry subjected to wall breaking; and (6) collection of the microalgae cells and recycling of the biogas slurry. According to the method, not only is a method provided for purifying the biogas slurry, but also the obtained algae cells and the biogas slurry can be recycled, so that ecologicalization treatment of the biogas slurry is realized, the environment is improved, and furthermore, the economic and social benefits are produced.
Description
Technical field
The present invention relates to the purifying method of a kind of natural pond liquid, particularly relate to a kind of natural pond based on both culturing microalgae liquid ecological purification method.
Background technology
Natural pond liquid, be otherwise known as " anaerobic fermented liquid " is that the organic matters such as animal excrement, stalk are residual after anaerobically fermenting has a water miscible by product.Along with agricultural structure adjustment and new countryside construction, intensive livestock and poultry breeding industry fast development, as the product natural pond liquid after biogas anaerobic fermentation, at present the generation in China every year is about more than 200,000,000 ton, these waste liquids are fertile or directly discharge as agriculture, whether seldom consider emission compliance and safety for agricultural use.In fact, natural pond liquid still contains a large amount of nitrogen, phosphorus and metal after anaerobic digestion, is regarded as high-concentration sewage.Take natural pond, pig farm liquid as example, its COD is still at 2000～4000mg/L, and total nitrogen is at 317～423mg/L, and total phosphorus is at 34.7～52.4mg/L, and total potassium, at 225～350mg/L, also has the heavy metals such as copper and zinc in addition, and directly discharging can cause body eutrophication.Along with the attention of country to environment protection, the strict control of sewage drainage standard, livestock and poultry farm discharged wastewater met the national standard and utilization have become the important factor that affects plant's survival and development.
Method for the treatment of city waste water is continued to use in the purifying method research of natural pond liquid more, adopt the methods such as physics, chemistry, biology, as the natural biology facture such as biological pond method and artificial swamp and adopt the industrial treatment pattern of the reactors such as MBR, SBR, reverse osmosis, but these methods are due to characteristics such as the too high and operative technique difficulty of running cost are large at present, be difficult to promote the raiser, and aforesaid method is not fairly obvious to the N in water, P removal effect, therefore, natural pond liquid directly discharges and remains main processing mode at present after simple Air Exposure.Also there are some that natural pond liquid is used for field irrigation, natural pond immersion kind, foliage-spray, soilless culture etc.; but due to transportation cost with use security problems; make the usage quantity of natural pond liquid seldom; a large amount of natural ponds liquid that the large-scale cultivation industry of can't dissolving anaerobically fermenting produces, therefore directly discharge the main processing mode that is still present natural pond liquid.
Summary of the invention
The purpose of this invention is to provide a kind of natural pond based on both culturing microalgae liquid ecological purification method, solve the unprocessed direct exhaust emission environment of a large amount of natural ponds liquid that present livestock and poultry cultivation produces, adopt that conventional sewage water treatment method cost is high, operation is difficult, denitrogenation, phosphor-removing effect are poor, the common problem that is difficult to promote.
A kind of natural pond based on both culturing microalgae liquid ecological purification method, comprise the steps:
(1) natural pond liquid pre-treatment: the natural pond liquid after anaerobically fermenting after solid-liquid separation, aerobic treatment, enters sterilization pool sterilization, equalizing tank carries out potential of hydrogen and nutritive ingredient adjustment successively, and the water of equalizing tank is introduced seed and spread cultivation in pond and open optical-biological reaction pond;
(2) domestication of the little algae of autotrophic type is cultivated: in concentration 30-100% scope, the natural pond liquid that the concentration of using raises successively, as nutrient solution, is cultivated little algae according to the method domestication of microbial acclimation, acquisition can be in the liquid of 70%-100% natural pond the algae strain of Fast Growth;
(3) preparation of seed liquor: the chlorella algae strain after taming is inoculated into open seed and spreads cultivation in pond and cultivate 3-5 days, obtains to be in the chlorella seed liquid of logarithmic phase, i.e. the algae kind;
(4) incubation growth of little algae coupling natural pond liquid purifies: the algae kind that is in logarithmic phase after spreading cultivation is inoculated in Race-way photobioreactor cultivates, and makes natural pond liquid reach the secondary wastewater discharge standard when obtaining high-density chlorella biomass;
(5) the biochemical broken wall of microalgae cell: the microalgae cell that will cultivate in the liquid of natural pond 7-9 days is introduced biochemical broken wall pond, and the fresh-water fishes of dropping into some amount and population cultivate, and chlorella is carried out broken wall treatment;
(6) gathering of microalgae cell: adopt the frustule after the flocculating settling method is collected broken wall, obtain the chlorella algae slurry, can be used for the production of high-protein feed additive; The top clear liquor be collected in pond as reuse water can be used for breeding fish, the purposes such as irrigation.
Natural pond based on both culturing microalgae of the present invention liquid ecological purification method, wherein step (1) is specially: the natural pond liquid after anaerobically fermenting enters aeration tank and carries out Air Exposure after solid-liquid separating machine separates, natural pond liquid after aeration enters sterilization pool, be that the 8-10% chlorine bleach liquor carries out disinfection with concentration, addition is 1-3mL/L, with the Sulfothiorine of same concentrations neutralize thereafter, described clorox stoste effective chlorine content % 〉=10.0;
The adjustment of described nutritive ingredient refers to the supplementary adjustment of lettuce, potential of hydrogen and nutritive salt composition; Described aeration process adds the unslaked lime of water body volume 0.3-0.5 ‰, and after continuous aeration 24h, interval 3 hours is continuous aeration 24h again, precipitation 2h.
Natural pond based on both culturing microalgae of the present invention liquid ecological purification method, wherein step (2) is specially: chlorella is placed in illumination box, in being 30%, 50%, 70%, 80% and 100% natural pond liquid, concentration tames successively, culture temperature is 23-25 ℃, pH6.5-7.5, illumination 3500-4000Lx; The well-grown algae strain of picking is inoculated in the natural pond liquid of greater concn from the liquid of lower concentration natural pond, and finally taming, filter out can the algae strain of Fast Growth in the liquid of 70-100% natural pond respectively.
Natural pond based on both culturing microalgae of the present invention liquid ecological purification method, wherein step (3) is specially: the chlorella algae strain after taming is inoculated into 20m
3Open seed spreads cultivation in pond and cultivates 3-5 days, nutrient solution is that natural pond liquid and fresh water mix according to the 5:5 ratio, according to SE formulated nutritive salt, the chlorella algae kind according to after the inoculum size access domestication of 3-5%, make its Initial seeding density reach ten thousand cells/ml of 1000-1500, concrete culture condition is pH6.8-7.5, culture temperature 23-25 ℃, intensity of illumination 3500-4000Lx, cultivated days are 3-5d, be as the criterion vegetative period to reach cell log, obtain to be in the chlorella seed liquid of logarithmic phase.
Natural pond based on both culturing microalgae of the present invention liquid ecological purification method, wherein step (4) is specially: the algae kind after spreading cultivation is inoculated into 100m
3Open optical-biological reaction pond in, inoculum size is 10-15%, inoculum density is 0.8-1.0 * 10
8It is 70%, 80% and 100% natural pond liquid that individual cells/ml, nutrient solution are selected respectively concentration;
Wherein, little algae culturing process adopts feed supplement fed-batch mode and semicontinuous cultural method: according to the micro algae growth curve, added one time of nutrition liquid at 4-5 days with reference to the SE formula, wherein the situation of adding of nitrogen salt and microcosmic salt is determined according to TN and TP concentration that real-time online detects; Cultural method adopts semicontinuous cultivation:, according to the change in concentration rule of COD, TN, TP in the liquid of natural pond, is chosen in 5-7 days pumps and walks the micro algae culturing liquid of 30%-50%, and the fresh natural pond liquid of supplementary equivalent, after this every 3-4 days, upgrade and replace once.
Natural pond based on both culturing microalgae of the present invention liquid ecological purification method, the culture temperature of wherein said chlorella is 22-26 ℃, illumination 5000-8000Lx, light application time 16h/d, pass into air speed 8-10vvm.
Natural pond based on both culturing microalgae of the present invention liquid ecological purification method, wherein step (5) is specially: the chlorella algae liquid that will cultivate in the liquid of natural pond 7-9 days is introduced biochemical broken wall pond, adds fresh-water fishes, and cultivation density is 1-3 bar/m
3, remove fish after 3 days, add flocculation agent precipitation frustule, collect the algae slurry of bottom, pond.
Natural pond based on both culturing microalgae of the present invention liquid ecological purification method, wherein said fresh-water fishes are silver carp, carp and crucian, and described flocculation agent is the alum of 200-300mg/mL, and during sedimentation, the pH value is 9-11.
Natural pond based on both culturing microalgae of the present invention liquid ecological purification method difference from prior art is:
(1) used little algae in the present invention, little algae is a class photo-autotroph, has that photosynthetic efficiency is high, the characteristics of fast growth, and effectively body eutrophication composition and the heavy metals such as absorbed nitrogen, phosphorus in its production process, reach the purpose that purifies natural pond liquid.The micro algae biomass of results is processing and utilization further, and the natural pond liquid after purifying can be used for irrigating, rinsing colony house etc., realizes resource and energy regeneration and the recycling of natural pond liquid, when improving environment, increases economic and social benefit.
(2) the invention solves the unprocessed direct exhaust emission environment of a large amount of natural ponds liquid that present livestock and poultry cultivation produces, adopts that conventional sewage water treatment method cost is high, the operation difficulty, denitrogenation, phosphor-removing effect are poor, the common problem that is difficult to promote; By a kind of method of utilizing little algae culturing process to reduce contamination index in the liquid of natural pond is provided, make it reach emission standard, described method not only purifies for a large amount of natural pond liquid the method that provides, and the natural pond liquid of the frustule of results after can further deeply adding and purify can be recycled, realized the ecology processing of natural pond liquid, produced economic and social benefit when improving environment.
(3), there is complicated operation in traditional physics, chemistry and closed bioreactor sewage water processing mode, and running cost is high, the shortcoming that should not promote, and the present invention adopts open pond both culturing microalgae method to purify waste water, and cost is low, and is simple to operate, very easily promotes.
(4) utilize the characteristics of autotrophic type micro algae growth process energy nitrogen use efficient phosphorus to dispose of sewage in the present invention, can effectively remove TN and TP in sewage, reduce COD, overcome in general sewage water treatment method the shortcoming to the poor removal effect of nitrogen phosphorus in water.
(5) adopt natural pond liquid to cultivate little algae, reach the purpose of purification of water quality in the harvesting microalgae biomass, the microalgae cell of results can continue to serve as the processing of feed etc., produce certain economic benefit, and the natural pond liquid after purifying can reuse be used for breeding fish, irrigation etc., realizes the ecology purification of water quality.
The present invention is based on main improvement in the natural pond liquid ecological purification method of both culturing microalgae is: at first little algae is cultivated with natural pond liquid and purifies and combine, be secondly little algae cultivate and natural pond liquid scavenging process in employing feed supplement stream add and semicontinuous cultural method; The general Closed photobioreactor that adopts is cultivated little algae in the method for existing report, the reactor complicated operation, cultivate cost high, is difficult for promoting, and it focuses on on the liquid cultivating microalgae of natural pond, and the present invention adopts open pond reactor, and equipment cost and running cost are all low, and is simple to operate, easily the raiser, promote, special emphasis is to make natural pond liquid can reach emission standard on purifying treatment to natural pond liquid, and harvesting microalgae is done its and used simultaneously.
In addition, aeration process in the method for the invention adds unslaked lime, can play the auxiliary protozoon that kills, simultaneously particle diameter can be removed larger, the material that biodegradability is not strong, interval 3 hours continuous aeration 24h again after continuous aeration 24h, different aeration modes produce different results, adopt aeration mode of the present invention more to help the breeding of aerobic bacteria, reduce the conversion of water body COD and organonitrogen phosphorus compound, improve the decontamination effect improving of little algae to water body in follow-up workshop section; The present invention adds fresh-water fishes to carry out biochemical broken wall in biochemical broken wall pond, replace traditional physics, the chemical processes such as ultrasonic wave, high-pressure homogenization or enzyme process, and is simple to operate, cost is low, and is effective.
Below in conjunction with accompanying drawing, the natural pond based on both culturing microalgae of the present invention liquid ecological purification method is described further.
Description of drawings
Fig. 1 is the schematic flow sheet of the natural pond based on both culturing microalgae of the present invention liquid ecological purification method;
Fig. 2 is chlorella growth figure in the liquid of different concns natural pond after domestication of the embodiment of the present invention.
Embodiment
Embodiment
As Fig. 1, the natural pond liquid ecological purification method that the present invention is based on both culturing microalgae comprises the steps:
1, natural pond liquid pre-treatment: the natural pond liquid after anaerobically fermenting is through Air Exposure, and the natural pond liquid after aeration enters sterilization pool after solid-liquid separating machine separates, and is the clorox 1-3mL/L sterilization of 8%-10% with concentration, the Sulfothiorine neutralization of same concentrations; Carry out the adjustment of nutritive ingredient in equalizing tank, refer to the supplementary adjustment of lettuce, potential of hydrogen and nutritive salt composition; The water of described equalizing tank is incorporated into seed spread cultivation pond and open optical-biological reaction pond.
Described aeration process adds the unslaked lime of 0.3-0.5 ‰, interval 3 hours continuous aeration 24h again after continuous aeration 24h; Described adjustment measure and little algae culture condition are complementary.
2, the domestication of the little algae of autotrophic type is cultivated: with photoautotrophy type Chlorella vulgaris (Chlorella mulgaris, available from Inst. of Hydrobiology, Chinese Academy of Sciences's algae kind storehouse FACHB) be that the algae kind is in concentration 30-100% scope, the natural pond liquid that the concentration of using raises successively is as nutrient solution, cultivate little algae according to the domestication of the method for microbial acclimation, acquisition can be in the liquid of 70%-100% natural pond the algae strain of Fast Growth; Different concns natural pond liquid domestication chlorella growth situation as shown in Figure 2.
Culture medium prescription is optimized, and, according to the content of total nitrogen (TN) in the liquid of initial natural pond and total phosphorus (TP), with reference to the SE formula, finely tunes, and makes NaNO in nutrient solution
3: 5-15g/100mL, K
2HPO4:3.0-7.5g/100mL, KH
2PO4:10-17.5g/100mL, other is constant.
The SE original formulation following (formula source: Inst. of Hydrobiology, Chinese Academy of Sciences's algae kind storehouse):
(1) NaNO
3: 25g/100mL), K
2HPO4:7.5g/100mL, MgSO
47H2O:7.5g/100mL, CaCl
22H
2O:2.5g/100mL, KH
2PO4:17.5g/100mL, NaCl:2.5g/100mL, FeCl
3.6H
2O:0.05g/100mL, above inorganic salt addition is 1mL/L.
(2) EDTA-Fe1mL/L, compound method is as follows: 1. 1N HCl: get the 4.1ml concentrated hydrochloric acid with distilled water diluting to 50ml; 2. 0.1N EDTA-Na takes 0.9306g and is dissolved in 50ml distilled water; 3. take FeCl
3.6H
2O0.901g is dissolved in the 1N HCl that the above step of 10ml prepared, and then with the 0.1N EDTA-Na that 10ml has prepared, mixes, and adds distilled water diluting to 1000ml.
(3) micro-1ml/L, fill a prescription as follows: H
3BO
32.86g/L dH
2O, MnCl
24H
2O1.86g/L, ZnSO
47H
2O0.22g/L, Na
2MoO
4.2H
2O0.39g/L, CuSO
4.5H
2O0.08g/L, Co (NO
3)
2.6H
2O0.05g/L.
(4) soil extract 40mL/L, the preparation method is as follows: get garden soil and do not execute overfertilization 200g and be placed in beaker or triangular flask, add 1000 milliliters of distilled water, bottleneck seals with porous plug, and the boiling water heating is 3 hours in water-bath, cooling, precipitate 24 hours, this process is carried out 3 times continuously, then filters, get supernatant liquor, save backup in 4 ℃ of refrigerators after sterilizing in high-pressure sterilizing pot.
3, the preparation of seed liquor (spreading cultivation): the chlorella algae strain after taming is inoculated into 20m
3Open seed spreads cultivation in pond and cultivates 3-5 days, nutrient solution is that natural pond liquid and fresh water mix according to the 5:5 ratio, according to SE formulated nutritive salt, according to the chlorella algae kind after the inoculum size access domestication of 3-5%, make its Initial seeding density reach ten thousand cells/ml of 1000-1500, concrete culture condition is pH6.8-7.5, culture temperature 23-25 ℃, intensity of illumination 3500-4000Lx, cultivated days is 3-5d, be as the criterion vegetative period to reach cell log, obtain to be in the chlorella seed liquid of logarithmic phase, i.e. the algae kind.
4, the incubation growth of little algae coupling natural pond liquid purifies: the algae kind after spreading cultivation is inoculated into 100m
3Open optical-biological reaction pond in, control little algae culture condition and cultural method, make the content of total nitrogen in the liquid of natural pond (TN), total phosphorus (TP), COD and poisonous metal reach " livestock and poultry breeding industry pollutant emission standard " (GB18596-2001) or the town sewage secondary discharge standard.Concrete operation step is as follows:
(1) be in the domestication chlorella algae strain of logarithmic phase according to the inoculum size access of 10-15%, the inoculation initial density is reached for 0.8-1.0 * 10
8Individual cells/ml.
(2) little algae culturing process adopts the feed supplement fed-batch mode, and lettuce originally selects respectively 70%, 80% and 100%., according to the micro algae growth curve, added one time of nutrition liquid at 4-5 days with reference to the SE formula, but the situation of adding of nitrogen salt and microcosmic salt is determined according to TN and TP concentration that real-time online detects.
(2) cultural method adopts semicontinuous cultural method, concentration according to COD, TN, TP in the liquid of natural pond, be chosen in the fresh natural pond liquid that adopted in 5-7 days and upgrade the micro algae culturing liquid that substitutes 30%-50%, after this every 3-4 days, carry out once method optimal way and carry out the purifying treatment of natural pond liquid.
In above-mentioned steps, the culture temperature of chlorella is 22-26 ℃, illumination 5000-8000Lx, and light application time 16h/d, pass into air speed 8-10vvm.Natural pond liquid is after growth cycle purifying treatment of chlorella, find that 70% natural pond liquid can reach best decontamination effect improving, as shown in table 1, each final pollution index value is total nitrogen 199.77mg/L, total phosphorus 2.14mg/L, ammonia nitrogen 1.59mg/L, active phosphorus 0.89mg/L, COD283.2mg/L, each index all reaches national town sewage secondary discharge standard.
Contamination index's contrast before and after table 1 natural pond liquid is processed
5, the biochemical broken wall of microalgae cell: the microalgae cell that will cultivate in the liquid of natural pond 7-9 days is introduced biochemical broken wall pond, cultivates fresh water fishes therein, adds silver carp, carp and crucian, and cultivation density is 1-3 bar/m
3, remove fish after 3 days, carry out biochemical broken wall.
6, gathering of microalgae cell: adopt flocculation agent sedimentation method precipitation frustule, described flocculation agent adopts the alum of 200-300mg/mL, and during sedimentation, pH adjusts to 9-11; In 2h, the rate of descent of chlorella cells can reach more than 85%.Collect the algae slurry of bottom, pond, can be used for the production of high-protein feed additive; The top clear liquor be collected in middle pond as reuse water can be used for breeding fish, the purposes such as irrigation.
Above-described embodiment is described the preferred embodiment of the present invention; not scope of the present invention is limited; design under the prerequisite of spirit not breaking away from the present invention; various distortion and improvement that those of ordinary skills make technical scheme of the present invention, all should fall in the definite protection domain of the claims in the present invention book.
Claims (8)
1. the liquid ecological purification method of the natural pond based on both culturing microalgae, is characterized in that: comprise the steps:
(1) natural pond liquid pre-treatment: the natural pond liquid after anaerobically fermenting after solid-liquid separation, aerobic treatment, enters sterilization pool sterilization, equalizing tank carries out potential of hydrogen and nutritive ingredient adjustment successively, and the water of equalizing tank is introduced seed and spread cultivation in pond and open optical-biological reaction pond;
(2) domestication of the little algae of autotrophic type is cultivated: in concentration 30-100% scope, the natural pond liquid that the concentration of using raises successively, as nutrient solution, is cultivated little algae according to the method domestication of microbial acclimation, acquisition can be in the liquid of 70%-100% natural pond the algae strain of Fast Growth;
(3) preparation of seed liquor: the chlorella algae strain after taming is inoculated into open seed and spreads cultivation in pond and cultivate 3-5 days, obtains to be in the chlorella seed liquid of logarithmic phase, i.e. the algae kind;
(4) incubation growth of little algae coupling natural pond liquid purifies: the algae kind that is in logarithmic phase after spreading cultivation is inoculated in open optical-biological reaction pond cultivates, and makes natural pond liquid reach the secondary wastewater discharge standard when obtaining high-density chlorella biomass;
(5) the biochemical broken wall of microalgae cell: the microalgae cell that will cultivate in the liquid of natural pond 7-9 days is introduced biochemical broken wall pond, and the fresh-water fishes of dropping into some amount and population cultivate, and chlorella is carried out broken wall treatment;
(6) gathering of microalgae cell: adopt the frustule after the flocculating settling method is collected broken wall, obtain the chlorella algae slurry, can be used for the production of high-protein feed additive; The top clear liquor be collected in pond as reuse water can be used for breeding fish, the purposes such as irrigation.
2. the natural pond based on both culturing microalgae according to claim 1 liquid ecological purification method, it is characterized in that: step (1) is specially: the natural pond liquid after anaerobically fermenting enters aeration tank and carries out Air Exposure after solid-liquid separating machine separates, natural pond liquid after aeration enters sterilization pool, be that the 8-10% chlorine bleach liquor carries out disinfection with concentration, addition is 1-3mL/L, with the Sulfothiorine of same concentrations neutralize thereafter, described clorox stoste effective chlorine content % 〉=10.0;
The adjustment of described nutritive ingredient refers to the supplementary adjustment of lettuce, potential of hydrogen and nutritive salt composition; Described aeration process adds the unslaked lime of water body volume 0.3-0.5 ‰, and after continuous aeration 24h, interval 3 hours is continuous aeration 24h again, precipitation 2h.
3. the natural pond based on both culturing microalgae according to claim 2 liquid ecological purification method, it is characterized in that: step (2) is specially: chlorella is placed in illumination box, in being 30%, 50%, 70%, 80% and 100% natural pond liquid, concentration tames successively, culture temperature is 23-25 ℃, pH6.5-7.5, illumination 3500-4000Lx; The well-grown algae strain of picking is inoculated in the natural pond liquid of greater concn from the liquid of lower concentration natural pond, and finally taming, filter out can the algae strain of Fast Growth in the liquid of 70-100% natural pond respectively.
4. the natural pond based on both culturing microalgae according to claim 3 liquid ecological purification method, it is characterized in that: step (3) is specially: the chlorella algae strain after taming is inoculated into 20m
3Open seed spreads cultivation in pond and cultivates 3-5 days, nutrient solution is that natural pond liquid and fresh water mix according to the 5:5 ratio, according to SE formulated nutritive salt, the chlorella algae kind according to after the inoculum size access domestication of 3-5%, make its Initial seeding density reach ten thousand cells/ml of 1000-1500, concrete culture condition is pH6.8-7.5, culture temperature 23-25 ℃, intensity of illumination 3500-4000Lx, cultivated days are 3-5d, be as the criterion vegetative period to reach cell log, obtain to be in the chlorella seed liquid of logarithmic phase.
5. according to claim 1 and 2 or 3 or 4 described natural pond based on both culturing microalgae liquid ecological purification methods, it is characterized in that: step (4) is specially: the algae kind after spreading cultivation is inoculated into 100m
3Open optical-biological reaction pond in, inoculum size is 10-15%, inoculum density is 0.8-1.0 * 10
8It is 70%, 80% and 100% natural pond liquid that individual cells/ml, nutrient solution are selected respectively concentration;
Wherein, little algae culturing process adopts feed supplement fed-batch mode and semicontinuous cultural method: according to the micro algae growth curve, added one time of nutrition liquid at 4-5 days with reference to the SE formula, wherein the situation of adding of nitrogen salt and microcosmic salt is determined according to TN and TP concentration that real-time online detects; Cultural method adopts semicontinuous cultivation:, according to the change in concentration rule of COD, TN, TP in the liquid of natural pond, is chosen in 5-7 days pumps and walks the micro algae culturing liquid of 30%-50%, and the fresh natural pond liquid of supplementary equivalent, after this every 3-4 days, upgrade and replace once.
6. the natural pond based on both culturing microalgae according to claim 5 liquid ecological purification method, it is characterized in that: the culture temperature of described chlorella is 22-26 ℃, illumination 5000-8000Lx, light application time 16h/d, pass into air speed 8-10vvm.
7. the natural pond based on both culturing microalgae according to claim 5 liquid ecological purification method, it is characterized in that: step (5) is specially: the chlorella algae liquid that will cultivate in the liquid of natural pond 7-9 days is introduced biochemical broken wall pond, adds fresh-water fishes, and cultivation density is 1-3 bar/m
3, remove fish after 3 days, add flocculation agent precipitation frustule, collect the algae slurry of bottom, pond.
8. the natural pond based on both culturing microalgae according to claim 7 liquid ecological purification method, it is characterized in that: described fresh-water fishes are silver carp, carp and crucian, and described flocculation agent is the alum of 200-300mg/mL, during sedimentation, the pH value is 9-11.
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| CN103922548A (en) * | 2014-05-04 | 2014-07-16 | 北京航空航天大学 | Biological reaction device for culturing high-oil microalgae by using wastewater |
| CN104388313A (en) * | 2014-10-22 | 2015-03-04 | 临沂大学 | Cultivation medium and method adopting crop straw retting liquid for cultivating Navichla corymbosa |
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