CN113321312A - High concentration of CO2Method for atmosphere domestication of microalgae high ammonia nitrogen resistant biogas slurry - Google Patents
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Abstract
The invention discloses high concentration CO2The method for acclimatizing microalgae high ammonia nitrogen resistant biogas slurry in atmosphere is characterized by comprising the following steps: step one, algae seed collection and culture: water area near farm for collecting biogas slurry to be treatedCulturing the microalgae in the culture medium; step two, microalgae enrichment culture: inoculating the microalgae solution obtained in the step one and the biogas slurry of a farm in a photobioreactor according to a certain proportion, and culturing under a proper condition, wherein CO is continuously introduced during culturing2Mixing the gas; step three, microalgae domestication: inoculating the microalgae solution obtained in the step two and the biogas slurry in the farm according to a certain proportion into a photobioreactor, and culturing under a proper condition, wherein CO is continuously introduced during culturing2Mixing the gas; step four, carrying out low-speed centrifugation treatment on the microalgae liquid obtained in the step three, and inoculating microalgae seeds at the bottom of a centrifugal tube and the biogas slurry in a farm to obtain finally domesticated high ammonia nitrogen resistant algae seeds; the invention can be widely applied to the fields of breeding industry, environmental protection and the like.
Description
Technical Field
The invention relates to the field of sewage treatment, in particular to high-concentration CO2A method for acclimatizing microalgae high ammonia nitrogen resistant biogas slurry.
Background
With the development of science and the progress of society, the living standard of people is continuously improved, the demand of people for meat food is increased day by day, and the livestock and poultry industry is developed in a large scale. However, the large-scale development of the livestock and poultry industry also brings a series of environmental problems. A large amount of sewage with high ammonia nitrogen, high COD and rich phosphorus needs to reach the discharge standard urgently, the instability of the water quality and the odor bring huge pressure to the environment, and the work of wastewater treatment faces more serious challenges.
Microalgae is a sewage purification organism with great potential, and can utilize nutrient substances such as ammonia nitrogen, phosphorus and the like in the livestock and poultry breeding sewage to grow so as to achieve the aim of purifying the sewage, so that the wastewater can be purified by culturing the microalgae. At the present stage, microalgae used by researchers for sewage treatment are generally pure algae cultured in laboratories, but the pure algae cannot be well adapted to complicated and variable biogas slurry environments with severe conditions, the sewage purification effect is not satisfactory, and the stability of the pure algae is greatly deficient when the pure algae is used for treating wastewater with high ammonia nitrogen and high concentration fluctuation. Based on the invention, natural microalgae are directly collected in a water area near a farm as the algae for treating biogas slurry. The water areas near the culturing farm are polluted by the biogas slurry of the culturing farm to different degrees, and can be approximately regarded as the biogas slurry diluted by many times, so that the microalgae growing in the water areas for a long time are adapted to the environment and have great potential for decomposing pollutants in the biogas slurry. In addition, the microalgae may be a collection of algal groups, and may also contain certain fungi. The fungi have certain protection and buffering effects on the wrapping of the microalgae, so that the microalgae has stronger adaptability to complex and variable environments, the characteristics of the microalgae adapting to the pig raising wastewater are facilitated, and higher denitrification and dephosphorization effects can be achieved. Therefore, the selection of microalgae in a water area near a farm as the species for treating biogas slurry can be a good scheme.
The problem that microalgae in a water area near a farm cannot adapt to a high ammonia nitrogen environment is also faced when the microalgae is used as the algae to treat biogas slurry. The pig farm wastewater has the characteristics of high concentration of organic matters and suspended matters, high ammonia nitrogen concentration, high treatment difficulty and the like, and although nutrient substances such as the rich ammonia nitrogen in the pig farm wastewater can be utilized by the growth of the microalgae, researches show that the growth of the microalgae can be obviously inhibited and even death can be caused when the concentration of the ammonia nitrogen exceeds 900 mg/L. This is because too high a concentration of ammonia nitrogen will produce more ammonium ions that cannot be rapidly transferred to the synthesis of amino acids, resulting in retarded growth or death of the microalgae. At present, the anaerobic-aerobic combined process is a traditional treatment mode adopted by the wastewater of the large-scale pig farm in China, and the biogas slurry fermented by the method still contains high-concentration nutrient substances such as nitrogen and phosphorus, wherein the concentration of ammonia nitrogen can reach 17-2050 mg/L, the content of COD is 1100-7100 mg/L, the content of total phosphorus is 2-300 mg/L, and the content is far higher than the water pollutant emission standard GB 18596 + 2001 of livestock and poultry breeding industry in China (the concentration of ammonia nitrogen is less than 80mg/L, the concentration of COD is less than 400mg/L, and the concentration of total phosphorus is less than 8 mg/L). The ammonia nitrogen concentration of the fermented biogas slurry still can exceed 900mg/L, and the growth of microalgae can still be inhibited. Microalgae in a water area near a farm adapts to the environment of the biogas slurry pollutants, but the adapted concentration is very low and cannot reach the level of directly treating the biogas slurry, but the ammonia nitrogen tolerance of the microalgae can be trained in a way of gradually increasing the concentration of the biogas slurry. The method improves the tolerance of microalgae to ammonia nitrogen by gradually increasing the concentration gradient domestication of the biogas slurry, so that the microalgae is suitable for the high ammonia nitrogen biogas slurry environment.
The problem of inhibition of microalgae on high ammonia nitrogen biogas slurry can be solved by adopting a gradient domestication mode, but the growth of microalgae needs to provide a corresponding carbon source. The carbon content in the microalgae cells reaches more than 50 percent, but the biogas slurry is only rich in nitrogen, phosphorus and other elements, but the carbon content is low, so that the growth requirement of the microalgae cannot be met. The waste water is treated by the traditional fermentation mode, a large amount of methane can be generated, the common utilization mode of the methane is direct combustion power generation, and the combustion of the methane can bring a large amount of high-concentration CO2Contaminated flue gas of containing CO2The flue gas is the optimal selection of the carbon source of the microalgae.
The microalgae in the invention can purify water by using pollutants such as ammonia nitrogen, phosphorus and the like in biogas slurry and can absorb CO in biogas combustion flue gas2And the mature microalgae can be processed after being harvested to obtain the algae-based multifunctional product. High concentration of CO2The method for acclimatizing the microalgae high ammonia nitrogen resistant biogas slurry has wide prospect in engineering application.
Disclosure of Invention
The technical problem to be solved by the invention is to provide high-concentration CO2A method for acclimatizing microalgae high ammonia nitrogen resistant biogas slurry.
The technical scheme of the invention is as follows: high concentration of CO2The method for acclimatizing microalgae high ammonia nitrogen resistant biogas slurry in atmosphere is characterized by comprising the following steps:
step one, algae seed collection and culture: collecting microalgae in a water area near a farm needing biogas slurry treatment, and culturing the microalgae;
step two, microalgae enrichment culture: inoculating the microalgae solution obtained in the step one and the biogas slurry of a farm in a photobioreactor according to a certain proportion, and culturing under a proper condition, wherein CO is continuously introduced during culturing2Mixing the gas;
step three, microalgae domestication: mixing the microalgae solution obtained in the step two with the biogas slurry in the farm according to the ratio of oneInoculating in a photobioreactor at a certain proportion, culturing under suitable conditions while continuously introducing CO2Mixing the gas;
step four, carrying out low-speed centrifugation treatment on the microalgae liquid obtained in the step three, inoculating microalgae seeds and the livestock farm biogas liquid at the bottom of a centrifugal tube into a serum bottle, placing the serum bottle in a constant-temperature greenhouse for culture, and continuously and quantitatively introducing CO during culture2And (3) supplying the mixed gas to a carbon source to obtain the final domesticated high ammonia nitrogen resistant algae.
High concentration of CO according to the invention2The preferable scheme of the method for acclimatizing microalgae high ammonia nitrogen resistant biogas slurry in atmosphere comprises the culture farm biogas slurry and CO-containing biogas slurry in the second, third and fourth steps2The mixed gas is prepared by the following steps:
A. anaerobic fermentation is carried out on the waste water of the farm to obtain biogas and biogas slurry of the farm;
B. burning the marsh gas to obtain electric energy and CO2And (4) mixing the gases.
High concentration CO according to the invention2The method for atmosphere domestication of the microalgae high ammonia nitrogen resistant biogas slurry has the beneficial effects that: the invention takes the natural microalgae in the water area around the farm to perform gradient acclimation treatment on the sewage, and continuously passes high-concentration CO2The purpose of deep purification of the sewage is achieved under the condition of (1), and the effect is obvious; the invention also utilizes high-concentration CO brought by the combustion of marsh gas generated by sewage fermentation2The flue gas is used as a carbon source, so that the treatment cost is reduced, and CO in the flue gas is treated2Besides, the invention can be widely applied to the fields of breeding industry, environmental protection and the like.
Drawings
FIG. 1 is a schematic view of a wastewater treatment process in a farm.
FIG. 2 shows high concentration CO according to the present invention2A flow schematic diagram of a method for acclimatizing microalgae high ammonia nitrogen resistant biogas slurry in atmosphere.
FIG. 3 is a statistical chart of ammonia nitrogen removal in examples 1, 2 and 3.
Detailed Description
The technical solution of the present invention is further described in detail with reference to the accompanying drawings and specific embodiments. However, it should be noted that the present invention is not limited to the following embodiments.
Referring to FIGS. 1 and 2, high concentration CO2The method for acclimatizing microalgae high ammonia nitrogen resistant biogas slurry in atmosphere comprises the following steps:
step one, algae seed collection and culture: collecting microalgae in a water area near a farm needing biogas slurry treatment, and culturing the microalgae;
step two, microalgae enrichment culture: inoculating the microalgae solution obtained in the step one and the biogas slurry of a farm in a photobioreactor according to a certain proportion, and culturing under a proper condition, wherein CO is continuously introduced during culturing2Mixing the gas;
step three, microalgae domestication: inoculating the microalgae solution obtained in the step two and the biogas slurry in the farm according to a certain proportion into a photobioreactor, and culturing under a proper condition, wherein CO is continuously introduced during culturing2Mixing the gas;
step four, carrying out low-speed centrifugation treatment on the microalgae liquid obtained in the step three, inoculating microalgae seeds and the livestock farm biogas liquid at the bottom of a centrifugal tube into a serum bottle, placing the serum bottle in a constant-temperature greenhouse for culture, and continuously and quantitatively introducing CO during culture2And (3) supplying the mixed gas to a carbon source to obtain the final domesticated high ammonia nitrogen resistant algae.
In a specific embodiment, the biogas slurry and CO-containing biogas slurry in the culturing farm in the second, third and fourth steps2The mixed gas is prepared by the following steps:
A. anaerobic fermentation is carried out on the waste water of the farm to obtain biogas and biogas slurry of the farm;
B. burning the marsh gas to obtain electric energy and CO2And (4) mixing the gases.
Example 1 high concentration of CO2The method for acclimatizing microalgae high ammonia nitrogen resistant biogas slurry in atmosphere comprises the following steps:
step 1: collecting and culturing algae
(1) Collection of algal species
Although the wastewater components in the pig farm plants are approximately the same, the wastewater components are different to some extent, and the microalgae groups most suitable for the wastewater characteristics should be present for the differences. The method directly collects and cultures the microalgae in the water area near the pig-raising factory which needs to treat biogas slurry, and the microalgae can be an aggregate of various algal groups and can also contain certain fungi. The method is favorable for the characteristics of the microalgae which are suitable for the pig raising wastewater, and can achieve higher denitrification and dephosphorization effects.
(2) The culture conditions are as follows: the temperature is 20-30 ℃, the illumination is 80-170 mu mol/(m2 & s), the pH is 6-10, and the growth period is 5-12 days.
Step 2: enrichment culture
Inoculating the natural microalgae algae solution and the pig farm biogas in the step 1 into a serum bottle according to the volume ratio of 1:1 (the pig raising wastewater accounts for 50%), and placing the bottle in a constant-temperature greenhouse for culturing, wherein the culture temperature is set to be 20-30 ℃, and the illumination is set to be 80-170 [ mu ] mol/(m ] mol2S) with constant quantitative introduction of CO2The carbon source was supplied with the mixed gas, and the algal solution was mixed, and the culture period was 6 days. 50 percent of the pig wastewater is purified by microalgae, the COD content in the effluent is 230mg/L, (the discharge standard GB 18596-3The removal rates of-N and TP reached 94% and 53.8%, respectively.
The step not only enriches the microalgae, but also has certain screening and domestication effects.
And step 3: microalgae domestication
Inoculating the microalgae solution obtained in the step 2 and the hogpen biogas slurry into a serum bottle according to the volume ratio of 1:3 (the waste water in the farm accounts for 75%), and placing the bottle in a constant-temperature greenhouse for culturing, wherein the culture temperature is set to be 20-30 ℃, and the illumination is set to be 80-170 [ mu ] mol/(m)2S) with constant quantitative introduction of CO2The carbon source was supplied with the mixed gas, and the algal solution was mixed, and the culture period was 7 days. 75 percent of the pig raising wastewater is decomposed by microalgae to obtain COD and NH in the effluent3The removal rates of-N and TP reach 86.7%, 98.5% and 100% respectively.
And 4, step 4: taking the microalgae liquid obtained in the step (3) for low-speed centrifugation treatment, taking microalgae seeds at the bottom of a centrifuge tube and inoculating the biogas slurry in a culture farm into a serum bottle(the culture wastewater accounts for 100 percent), and the culture solution is placed in a constant temperature greenhouse for culture, the culture temperature is set to be 20-30 ℃, and the illumination is set to be 80-170 mu mol/(m)2S) with constant quantitative introduction of CO2Supplying carbon source to the mixed gas, mixing the algae solution, and culturing for 7 days to obtain final domesticated algae. Decomposing 100 percent of aquaculture wastewater by microalgae to obtain COD and NH in effluent3The removal rates of-N and TP reach 84.5%, 86.4% and 100% respectively.
Example 2 high concentration of CO2The method for acclimatizing microalgae high ammonia nitrogen resistant biogas slurry in atmosphere comprises the following steps:
step 1: collecting and culturing conditions of algae
Directly collecting microalgae in a water area near a cultivation plant which needs to treat biogas slurry for cultivation, wherein the cultivation conditions are as follows: the temperature is 20-30 ℃, and the illumination is 80-170 mu mol/(m)2S) with a pH of 6-10 and a growth cycle of 5-12 days.
Step 2: enrichment culture
Inoculating the natural microalgae algae solution and the pig farm biogas slurry in the step 1 into a serum bottle according to the volume ratio of 1:1 (the pig raising wastewater accounts for 50%), and placing the bottle in a constant temperature greenhouse for culturing, wherein the culture temperature is set to be 20-30 ℃, and the illumination is set to be 80-170 [ mu ] mol/(m ] mol2S) with constant quantitative introduction of CO2The carbon source was supplied with the mixed gas, and the algal solution was mixed, and the culture period was 6 days. 50 percent of the pig raising wastewater is decomposed by microalgae to obtain COD and NH in the effluent3The removal rates of-N and TP reach 63.3%, 88.2% and 60%, respectively.
The step not only enriches the microalgae, but also has certain screening and domestication effects.
And step 3: microalgae domestication
Inoculating the microalgae solution obtained in the step 2 and the hogpen biogas slurry into a serum bottle according to the volume ratio of 1:3 (the pig raising wastewater accounts for 75%), and placing the bottle in a constant-temperature greenhouse for culturing, wherein the culture temperature is set to be 20-30 ℃, and the illumination is set to be 80-170 [ mu ] mol/(m ] mol2S) with constant quantitative introduction of CO2The carbon source was supplied with the mixed gas, and the algal solution was mixed, and the culture period was 7 days. 75 percent of the pig raising wastewater is decomposed by microalgae to be dischargedCOD and NH of3The removal rates of-N and TP reach 89.9%, 82.7% and 100% respectively.
And 4, step 4: and (3) carrying out low-speed centrifugation treatment on the microalgae liquid obtained in the step (3), inoculating microalgae seeds at the bottom of a centrifuge tube and the biogas slurry in a farm into a serum bottle (the pig wastewater accounts for 100%), placing the bottle in a constant-temperature greenhouse for culture, setting the culture temperature to be 20-30 ℃ and setting the illumination to be 80-170 [ mu ] mol/(m & lt/& gt)2S) with constant quantitative introduction of CO2And supplying a carbon source to the mixed gas, mixing the algae liquid, and culturing for 5-12 days to obtain the finally domesticated algae. Decomposing 100 percent of pig raising wastewater by microalgae to obtain COD and NH in effluent3The removal rates of-N and TP reach 92%, 89.7% and 100% respectively.
Example 3 high concentration of CO2The method for acclimatizing microalgae high ammonia nitrogen resistant biogas slurry in atmosphere comprises the following steps:
step 1: collecting and culturing algae
Directly collecting microalgae in a water area near a cultivation plant which needs to treat biogas slurry for cultivation, wherein the cultivation conditions are as follows: the temperature is 20-30 ℃, and the illumination is 80-170 mu mol/(m)2S) with a pH of 6-10 and a growth cycle of 5-12 days.
Step 2: enrichment culture
Inoculating the natural microalgae algae solution and the pig farm biogas slurry in the step 1 into a serum bottle according to the volume ratio of 1:1 (the pig raising wastewater accounts for 50%), and placing the bottle in a constant temperature greenhouse for culturing, wherein the culture temperature is set to be 20-30 ℃, and the illumination is set to be 80-170 [ mu ] mol/(m ] mol2S) and continuously and quantitatively feeding a mixed gas containing CO2 to supply a carbon source, and mixing the algae solution, wherein the culture period is 6 days. 50 percent of the pig raising wastewater is decomposed by microalgae to obtain COD and NH in the effluent3The removal rates of-N and TP reach 56.9%, 87.4% and 96.1%, respectively.
The step not only enriches the microalgae, but also has certain screening and domestication effects.
And step 3: microalgae domestication
Inoculating the microalgae solution obtained in the step 2 and the hogpen biogas slurry into a serum bottle according to the volume ratio of 1:3 (the pig raising wastewater accounts for 75 percent), and placing the bottle at a constant temperatureCulturing in a greenhouse at 20-30 deg.C and illumination at 80-170 μmol/(m)2S) with constant quantitative introduction of CO2The carbon source was supplied with the mixed gas, and the algal solution was mixed, and the culture period was 6 days. 75 percent of the pig raising wastewater is decomposed by microalgae to obtain COD and NH in the effluent3The removal rates of-N and TP reach 70%, 98.7% and 100% respectively.
Step 4, performing low-speed centrifugation treatment on the microalgae liquid obtained in the step 3, inoculating the microalgae seeds at the bottom of a centrifuge tube and the pig farm biogas liquid into a serum bottle (the pig raising wastewater accounts for 100%), and placing the bottle in a constant-temperature greenhouse for culture, wherein the culture temperature is set to be 20-30 ℃, and the illumination is set to be 80-170 [ mu ] mol/(m ] mol2S) and continuously and quantitatively feeding a CO 2-containing mixed gas to supply a carbon source, mixing the algae solution, and culturing for 7 days to obtain the final domesticated algae. Decomposing 100 percent of pig raising wastewater by microalgae to obtain COD and NH in effluent3The removal rates of-N and TP reach 84.5%, 86% and 100% respectively.
Referring to fig. 3, the method of the invention has the advantages of low cost, simple operation, good denitrification and dephosphorization effect and extremely high success rate. While treating the biogas slurry wastewater, the device can also absorb CO generated by the combustion of biogas generated in the fermentation process2。
While embodiments of the invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.
Claims (2)
1. High concentration of CO2The method for acclimatizing microalgae high ammonia nitrogen resistant biogas slurry in atmosphere is characterized by comprising the following steps:
step one, algae seed collection and culture: collecting microalgae in a water area near a farm needing biogas slurry treatment, and culturing the microalgae;
step two, microalgae enrichment culture: inoculating the microalgae solution obtained in the step one and the biogas slurry in a culture farm according to a certain proportion into a photobioreactor, culturing under a proper condition,continuously introducing CO during culture2Mixing the gas;
step three, microalgae domestication: inoculating the microalgae solution obtained in the step two and the biogas slurry in the farm according to a certain proportion into a photobioreactor, and culturing under a proper condition, wherein CO is continuously introduced during culturing2Mixing the gas;
step four, carrying out low-speed centrifugation treatment on the microalgae liquid obtained in the step three, inoculating microalgae seeds and the livestock farm biogas liquid at the bottom of a centrifugal tube into a serum bottle, placing the serum bottle in a constant-temperature greenhouse for culture, and continuously and quantitatively introducing CO during culture2And (3) supplying the mixed gas to a carbon source to obtain the final domesticated high ammonia nitrogen resistant algae.
2. The high concentration CO of claim 12The method for atmosphere domestication of microalgae high ammonia nitrogen resistant biogas slurry is characterized by comprising the following steps: biogas slurry and CO-containing biogas slurry in farms in the second, third and fourth steps2The mixed gas is prepared by the following steps:
A. anaerobic fermentation is carried out on the waste water of the farm to obtain biogas and biogas slurry of the farm;
B. burning the marsh gas to obtain electric energy and CO2And (4) mixing the gases.
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