CN108611276A - A method of recycling treatment being carried out to distillery waste water using microalgae - Google Patents
A method of recycling treatment being carried out to distillery waste water using microalgae Download PDFInfo
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- CN108611276A CN108611276A CN201810339169.8A CN201810339169A CN108611276A CN 108611276 A CN108611276 A CN 108611276A CN 201810339169 A CN201810339169 A CN 201810339169A CN 108611276 A CN108611276 A CN 108611276A
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- microalgae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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Abstract
The invention discloses a kind of methods carrying out recycling treatment to distillery waste water using microalgae.The method of the present invention includes the following steps：Using distillery waste water as fermentation substrate, activated sludge is added and ferments, after fermentation, obtains zymotic fluid；Microalgae is inoculated in culture medium and is cultivated；Microalgae after culture is inoculated in zymotic fluid and carries out Heterotrophic culture, obtains microalgae.The present invention carries out recycling treatment using microalgae to distillery waste water, both microalgae can be cultivated, the gas generated in distillery waste water fermentation process can do combustion gas, also organic substance is further decomposed and is utilized for microdisk electrode, the resource of microdisk electrode has been saved, meanwhile, the bulky grain in treated distillery waste water is decomposed, become clear, is convenient for the processing work of follow-up discharge of wastewater.
Description
Technical field
The invention belongs to distillery waste water treatment technology and environmental technology fields, it is more particularly related to a kind of utilization
The method that microalgae carries out recycling treatment to distillery waste water.
Background technology
Schlempe draff liquid waste water is the waste water that wine brewing largely generates in the process can give birth to its main feature is that COD, nitrogen and phosphorus content are high
The property changed is good, and water environment safety can be seriously affected if do not handled.In people in the long felt and experiment of draff liquid purification method,
The sewage purification system based on anaerobic-aerobic biological degradation method is gradually formed.Anaerobic-aerobic biological degradation method, high concentration have
Machine waste water is degraded into the compounds such as acetic acid, ethyl alcohol, carbon dioxide and hydrogen under anaerobic, by larger molecular organics, then
It is converted it into as methane by methane backeria.After the lower COD value of anaerobic condition processing declines to a great extent, after Air Exposure, waste water COD
Value can substantially meet discharging standards.The master that anaerobic-aerobic biological degradation method is also handled at draff liquid in world wide
Want method.But as processing cost and waste water reclaiming develop, this treatment technology exist removal N, P etc. effect compared with
The shortcomings of methane gas recycling storage utilization of difference, generation is more difficult, and cost is higher.
Microalgae refer to it is a kind of small, it is simple in structure, grow rapid, the adaptable overwhelming majority can carry out it is photosynthetic from
Foster rudimentary plant.Its nutritional mode is largely photoautotrophy, but also has a large amount of chmosynthetic heterotrophs.Microalgae cell is past
Contact can have the bioactive substance of health care and pharmacological action containing multiple proteins, antibiotic, vitamin, aliphatic acid etc.,
Application potential with exploitation high value added product.
Invention content
It is an object of the invention to：Overcome above-mentioned deficiency existing in the prior art, provides a kind of microalgae to distillery waste water
The method for carrying out recycling treatment.
In order to achieve the above-mentioned object of the invention, recycling treatment being carried out to distillery waste water using microalgae the present invention provides a kind of
Method comprising following steps：
(1) using distillery waste water as fermentation substrate, activated sludge is added and ferments, after fermentation, obtains zymotic fluid；
(2) microalgae is inoculated in culture medium and is cultivated；
(3) microalgae after step (2) described culture is inoculated in step (1) described zymotic fluid and carries out Heterotrophic culture, obtained
To microalgae；
Wherein, the preparation method of step (1) described activated sludge is：Take fresh sludge, removal big from sewage treatment plant
It after granule foreign and rubbish, precipitates 30min or more and excludes impurity, remove supernatant, the vinasse that dilution 50% is then added are useless
After water carries out anaerobism preculture 5h, removes supernatant, obtain activated sludge.
Carry out a kind of optimal technical scheme of the method for recycling treatment, step to distillery waste water using microalgae as the present invention
Suddenly in (1), the temperature of the fermentation is 29 ± 1 DEG C, and the time of the fermentation is 16~68h.
Carry out a kind of optimal technical scheme of the method for recycling treatment, step to distillery waste water using microalgae as the present invention
Suddenly in (2), the culture medium includes following component：NH4Cl 600mg/L, KH2PO470mg/L, K2HPO488mg/L,
MgSO4·7H2O 80mg/L, CaCl 50mg/L.
Carry out a kind of optimal technical scheme of the method for recycling treatment, step to distillery waste water using microalgae as the present invention
Suddenly in (2), the culture is stationary culture under illumination condition, and intensity of illumination is 3000~4500Lux, is vibrated 3~4 times daily,
Cultivation temperature is 20~30 DEG C, is cultivated 6 days.
Carry out a kind of optimal technical scheme of the method for recycling treatment, step to distillery waste water using microalgae as the present invention
Suddenly in (3), the Heterotrophic culture is cultivated 5 days at 15~30 DEG C.
Carry out a kind of optimal technical scheme of the method for recycling treatment to distillery waste water using microalgae as the present invention, it will
The gas collection generated in step (1) described fermentation process is passed through described after the completion of step (3) described Heterotrophic culture
Gas carries out autotrophy culture.
Carry out a kind of optimal technical scheme of the method for recycling treatment, institute to distillery waste water using microalgae as the present invention
The temperature for stating autotrophy culture is 15~30 DEG C, and intensity of illumination is 4000~8000Lux, is cultivated 7 days.
Wherein, the autotrophic freshwater microalgae after domestication may be selected in microalgae of the present invention, can be in major algae collection
Or be commercially available by commercially available, chlorella, scenedesmus or spirulina specifically may be selected.
Compared with the existing technology, the invention has the advantages that：
(1) present invention generates methane, the i.e. main component of biogas by the distillery waste water that ferments, and can be collected as combustion
Gas.
(2) present invention makes bulky grain therein decompose, waste water becomes clear, after being convenient for by the distillery waste water that ferments
Continuous discharge of wastewater processing work.
(3) present invention is by the distillery waste water that ferments, and larger molecular organics therein are converted into small molecule, and fermentation process is produced
Raw carbon dioxide can further give the growth of microalgae to provide nutriment, save the cost of microdisk electrode.
(4) two stages of fermentation process and microdisk electrode are combined by the method for the present invention, can both cultivate to obtain high-biomass,
The microalgae of high fat content, and combustion gas can be obtained, also nutriment can be provided for micro algae growth, distillery waste water is made to turn waste into wealth,
Reduce environmental pollution, realizes the recycling of distillery waste water.
Specific implementation mode
In order to make the purpose of the present invention, technical solution and advantageous effects be more clear, with reference to embodiments, to this
Invention is further elaborated.It should be understood that embodiment described in this specification is just for the sake of this hair of explanation
It is bright, be not intended to limit the present invention, parameter, ratio of embodiment etc. can adaptation to local conditions make a choice and substance had no to result
It influences.
Embodiment 1
Step 1：Wine lees liquor ferments.It using 500g wine lees liquors as fermentation substrate, is loaded in reaction unit, volume is pressed in inoculation
Than to start to ferment after 15% activated sludge (being derived from sewage treatment plant), temperature is controlled at 29 ± 1 DEG C, and the gas collection of generation exists
In the air accumulator of 2.5L, after the 30h that ferments, it is separated by solid-liquid separation tunning is clarified.After testing, gas production in the process
About 200ml/d, wherein methane content are 50%, other gases are carbon dioxide, hydrogen sulfide etc..
The preparation of activated sludge：Fresh sludge () g is taken from sewage treatment plant, after removing large granular impurity and rubbish, is sunk
Shallow lake 30min or more simultaneously excludes impurity, removes supernatant, and wine lees liquor () g that dilution 50% is then added carries out anaerobism preculture 5h
Afterwards, remove supernatant, obtain activated sludge.
Step 2：The culture of algae：The algae used (is purchased from Univ Minnesota-Twin Cities USA for pirulirua plalensi
Algae collection, UTEX 3680).Medium component is NH4Cl 600mg/L, KH2PO470mg/L, K2HPO488mg/L,
MgSO4·7H2O 80mg/L, CaCl250mg/L, when inoculum concentration 10%, microalgae in the culture medium total fat content up to cell
30% or more of dry weight is oil-rich microalgae.The algae, which has been verified by experiments, to survive in the liquid obtained by acidogenic fermentation.Specific behaviour
As：Culture medium is added separately in conical flask (tool breathable sealing film), algae is accessed through sterile working after 121 DEG C of sterilizings
Kind, it is subsequently placed in stationary culture in illumination box, daily concussion 3 times, cultivation temperature is 25 DEG C, intensity of illumination 3000Lux
(fluorescent lamp) is cultivated 6 days.
Step 3：Heterotrophic culture：It takes in the supernatant to fermented and cultured device after being separated by solid-liquid separation, according to 10% inoculum concentration
(algae OD680To be cultivated after algae obtained by 0.8) the above-mentioned culture of access.Condition of culture is 28 DEG C of temperature, air agitation culture
It is 15g/L, fat content 31% that biomass is measured by sampling after 5 days.
Embodiment 2
The present embodiment after three Heterotrophic cultures, is continued autotrophy process, comprises the concrete steps that embodiment 1 the step of：
Microalgae after Heterotrophic culture is placed in pot type bioreactor, cycle be passed through storage gas tank in gaseous mixture into
Row autotrophy culture, condition of culture are 25 DEG C of temperature, and intensity of illumination 5000Lux measures micro algae biomass 16g/L, grease after 7 days
Content is 33%.
Embodiment 3
Step 1：Drunk fish ferments.It using 500g drunk fish as fermentation substrate, is loaded in reaction unit, is inoculated with
Start to ferment after 25% activated sludge (being derived from methane-generating pit), temperature control is at 29 ± 1 DEG C, and the gas collection of generation is in storage gas tank
In, it is after the 68h that ferments, tunning is clarified.After testing, gas production about 250ml/d, wherein methane content are in the process
50%, other gases are carbon dioxide, hydrogen sulfide etc..Life is measured by sampling with the step two in embodiment 1 in remaining step after 5 days
Object amount is 17g/L, fat content 36%.
Embodiment 4
Step 1：Drunk fish ferments.It is loaded in reaction unit using 2L drunk fish as fermentation substrate, inoculation 20% is lived
Property sludge (being derived from methane-generating pit) after start to ferment, temperature is controlled at 29 ± 1 DEG C, and the gas collection of generation is in air accumulator, fermentation
After 16h, it is separated by solid-liquid separation tunning is clarified.After testing, gas production about 120ml/d, wherein methane in the process
Content is 40%, other gases are carbon dioxide, hydrogen sulfide etc..Remaining is measured by sampling with the step two in embodiment 1 after 5 days
Biomass is 12g/L, fat content 33%.
According to the disclosure and teachings of the above specification, those skilled in the art in the invention can also be to above-mentioned embodiment party
Formula carries out change and modification appropriate.Therefore, the invention is not limited in specific implementation modes disclosed and described above, to this
Some modifications and changes of invention should also be as falling into the scope of the claims of the present invention.In addition, although this specification
In used some specific terms, these terms are merely for convenience of description, does not limit the present invention in any way.
Claims (7)
1. a kind of method carrying out recycling treatment to distillery waste water using microalgae, which is characterized in that include the following steps：
(1) using distillery waste water as fermentation substrate, activated sludge is added and ferments, after fermentation, obtains zymotic fluid；
(2) microalgae is inoculated in culture medium and is cultivated；
(3) microalgae after step (2) described culture is inoculated in step (1) described zymotic fluid and carries out Heterotrophic culture, obtained micro-
Algae；
Wherein, the preparation method of step (1) described activated sludge is：Fresh sludge is taken from sewage treatment plant, removes bulky grain
After impurity and rubbish, precipitation 30min or more simultaneously exclude impurity, remove supernatant, then be added dilution 50% distillery waste water into
After row anaerobism preculture 5h, removes supernatant, obtain activated sludge.
2. the method according to claim 1 for carrying out recycling treatment to distillery waste water using microalgae, which is characterized in that step
Suddenly in (1), the temperature of the fermentation is 29 ± 1 DEG C, and the time of the fermentation is 16~68h.
3. the method according to claim 1 for carrying out recycling treatment to distillery waste water using microalgae, which is characterized in that step
Suddenly in (2), the culture medium includes following component：NH4Cl 600mg/L, KH2PO470mg/L, K2HPO488mg/L, MgSO4·
7H2O 80mg/L, CaCl 50mg/L.
4. the method according to claim 1 for carrying out recycling treatment to distillery waste water using microalgae, which is characterized in that step
Suddenly in (2), the culture is stationary culture under illumination condition, and intensity of illumination is 3000~4500Lux, is vibrated 3~4 times daily,
Cultivation temperature is 20~30 DEG C, is cultivated 6 days.
5. the method according to claim 1 for carrying out recycling treatment to distillery waste water using microalgae, which is characterized in that step
Suddenly in (3), the Heterotrophic culture is cultivated 5 days at 15~30 DEG C.
6. the method according to claim 1 for carrying out recycling treatment to distillery waste water using microalgae, which is characterized in that will
The gas collection generated in step (1) described fermentation process is passed through described after the completion of step (3) described Heterotrophic culture
Gas carries out autotrophy culture.
7. the method according to claim 6 for carrying out recycling treatment to distillery waste water using microalgae, which is characterized in that institute
The temperature for stating autotrophy culture is 15~30 DEG C, and intensity of illumination is 4000~8000Lux, is cultivated 7 days.
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Cited By (2)
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| CN110029066A (en) * | 2019-03-14 | 2019-07-19 | 北京联合大学 | A method of utilizing beer waste water culture chlorella |
| CN112707510A (en) * | 2020-12-08 | 2021-04-27 | 河海大学 | Method for coupling furfural wastewater treatment and microalgae culture |
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