CN106282020A - A kind of method of the autotrophic denitrification micropopulation of orienting enriching different proportion - Google Patents
A kind of method of the autotrophic denitrification micropopulation of orienting enriching different proportion Download PDFInfo
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Abstract
The method that the invention discloses the autotrophic denitrification micropopulation of a kind of orienting enriching different proportion.After the present invention can be by simple cultivation mud alive, utilize packing property can accelerate the formation of autotrophic denitrification mud, further may select and mould different types of denitrifying sludge, the autotrophic denitrification micropopulation being dominant with ammonia oxidation microbiological such as formation, efficiently complete the work that mineralized nitrogen is nitrite, and form the autotrophic denitrification micropopulation being dominant with anaerobic ammonia oxidation microbiological, efficiently complete the work that total nitrogen is removed, therefore, the present invention designs corresponding filler and reaction unit according to its selectivity characteristic, orientation segmentation selects autotrophic denitrification microorganism, it is made to carry out more efficient deposit efficiency on different functional stuffings, improve its activity, i.e. ammonia oxidation microbiological is carried out selective enrichment with anaerobic ammonia oxidation microbiological and separates, it is thus possible to remove natural water under micro-oxygen conditions, ammonia nitrogen in municipal wastewater and total polluted by nitrogen.
Description
Technical field
The invention belongs to water process and environmental technology field, be specifically related to a kind of with magnetic iron ore, modified zeolite orienting enriching
The method of autotrophic denitrification microorganism.
Background technology
At present, the water environment pollution problem of China receives significant attention, ammonia nitrogen, total nitrogen, and Organic substance is dirty with novel toxic
The problem of dye thing emerges in an endless stream.In " ten, water " that country is new, coastal region and above city are implemented total nitrogen total emission volumn control
Requirement processed so that the requirement processing water improves a higher level.
For the pollutant-ammonia nitrogen accounting for water body total nitrogen more than 60%, derive from the degraded of all kinds of nitrogen-containing pollutant
Journey.It is said that in general, ammonia nitrogen only converts under the effect of ammonia oxidation microbiological, its transformation time is relevant to dissolved oxygen concentration,
Its accumulation often causes further anoxia at polluted-water, and anoxia has the accumulation further resulting in ammonia nitrogen.Additionally, ammonia nitrogen is water
Nutrient in body, may result in body eutrophication, simultaneously to Fish and many most toxic property of aquatile.Therefore, people
Improvement for ammonia and nitrogen pollution conducts extensive research.After the method using excess aeration removes ammonia nitrogen, cause a large amount of ammonia
Nitrogen transformation is nitrate, and the problem making water body total nitrogen too high starts to emerge.Water body denitrification is removed with trace contaminant and is mainly passed through
The metabolic activity of microorganism completes.The removal process of traditional microbiological total nitrogen needs to carry out denitrification again through ammoxidation, nitrification
Process.This process needs a large amount of aerations to be nitrate by mineralized nitrogen, then utilizes denitrification to remove nitre under organic conditions
Hydrochlorate.Remove completely and need to consume a large amount of electric energy and carbon source (methanol).Process the most serious ammonia and nitrogen pollution, allowed and unbearably weighed
Negative water treatment system energy consumption increases severely, and the appearance of emerging pollutant, the pressure processed will be increased the weight of further.At present, novel
Denitrogenation technology is always the forward position of environmental project research.Autotrophic denitrification technique is exactly that one of which has the de-of efficient energy-saving very much
Nitrogen technology.Autotrophic denitrification technology can remove ammonia nitrogen and total nitrogen under conditions of organic carbon source lacking, be greatly saved power consumption with
Carbon source consumes, and is therefore the most promising denitrification process of one.Therefore, exploitation one is directly applied for community or country sewage
Pretreatment, can remove acetaminophen again can simultaneous removing total nitrogen, the water treatment system of novel energy-saving environment-friendly of ammonia nitrogen, the most favorably
Dirty Urban Water pollution situation cannot be cut, it helps improve the safety of water environment, to following 3-5 distributing in alleviating to improve
The development of water technology has great importance.
But, owing to the doubling time of autotrophic denitrification micropopulation is considerably long, starting autotrophic denitrification micropopulation needs phase
When the long time, it is allowed to be restricted at engineer applied.How to accelerate the key that its incubation is practical application.
Summary of the invention
It is an object of the invention to provide a kind of ammonia nitrogen that can remove in natural water, municipal wastewater under micro-oxygen conditions with total
The method of the autotrophic denitrification micropopulation of the orienting enriching different proportion of polluted by nitrogen.
The method of the autotrophic denitrification micropopulation of the orienting enriching different proportion of the present invention, it is characterised in that include following
Step:
A, the modification of filler
The modification of magnetic iron ore filler: after the Magnet mining water of particle diameter 0.5～1cm is cleaned up, then change with soak with hydrochloric acid
Property, the magnetic iron ore after final rinse water hydrochloric Acid Modification to pH=7 is neutral, dries, and obtains modified magnetic iron ore filler；
The modification of zeolite filler: after the zeolite filler water of particle diameter 0.5-1cm is cleaned up, then change with soak with hydrochloric acid
Property, the zeolite filler after final rinse water hydrochloric Acid Modification to pH=7 is neutral, then soaks modification by NaOH solution, finally uses water
Rinse to pH=7 neutral, dry, obtain modified zeolite filler；
B, the preparation of autotrophic denitrification mud
Take the VSS concentration activated sludge more than 1mg/L, after excluding impurity, precipitation, then replace supernatant with M0 culture fluid
Liquid, continuous aeration and stirring so that it is dissolved oxygen is 2-3mg/L, when ammonia nitrogen degradation efficiency reaches more than 90%, Nitrite accumulation
When rate reaches more than 30%, change M0 culture fluid and continue to cultivate, continuous aeration and stirring so that it is dissolved oxygen is 2～3mg/L, directly
Reaching more than 90% to ammonia nitrogen degradation efficiency, nitrite accumulation rate reaches more than 60%, it is thus achieved that mud A；
Take mud A and move in another one container, aeration so that it is dissolved oxygen is 0.5～1mg/L, when ammonia nitrogen degradation efficiency
When reaching 70～80%, when nitrite degradation rate reaches more than 70%, change M1 culture fluid and continue to cultivate, continuous aeration and stirring
Mixing so that it is dissolved oxygen is 0.5～1mg/L, until ammonia nitrogen degradation efficiency reaches more than 90%, nitrite accumulation rate disappears, and obtains
Obtain mud B, be autotrophic denitrification mud；
Described M0 culture fluid every liter contains: NH4Cl 0.1g, NaHCO30.2g, surplus is water, pH7.0-7.5；
Described M1 culture fluid every liter contains: NH4Cl 0.5g, NaNO20.5g, NaHCO30.5g, surplus is water,
pH7.5-8.0；
The filling of C, reaction unit and filler
Reaction unit includes preaeration district that tandem is connected, inhalant region, modified zeolite packing area, modified magnetic iron ore
Packing area and water outlet settling zone, the front end in described preaeration district is provided with water inlet, and the tail end of described water outlet settling zone is provided with
Outlet, equipped with preaerator in preaeration district, described modified zeolite packing area is equipped with modified zeolite filler, described
Modified magnetic iron ore packing area is equipped with modified magnetic iron ore filler；
D, the startup of reaction unit and operation: the modified zeolite filler that mud B is inoculated in reaction unit and modified magnetic
On iron mine filler, then carry out biofilm, then reaction unit uses continuous stream mode startup optimization.
The modification of described magnetic iron ore filler is to be washed with water by the magnetic iron ore of particle diameter 0.5～1cm, until supernatant is clarified,
Then it is soaked in water 20～35min, then with the ultrasonic 30-60min of 40kHz, adding hydrochloric acid to pH is 3, then soaks 20-
30min, final rinse water to pH=7 is neutral, 120 DEG C of drying, obtains modified magnetic iron ore filler；
The modification of described zeolite filler is to be cleaned by the zeolite filler water of particle diameter 0.5-1cm, then uses 40kHz to surpass
Sound 30-60min, adds and rinses to pH=7 neutral with water after soaking 20-35min in hydrochloric acid, add mass fraction 10%
NaOH solution soak 30min, pH adjust to more than 12, soak zeolite filler 12h, final rinse water to neutral pH=7,120
DEG C dry, obtain modified zeolite filler.
Described reaction unit uses continuous stream mode startup optimization, and it concretely comprises the following steps:
Reaction unit is divided into two stages, the first stage for remove the ammonia nitrogen stage, second stage be total nitrogen, ammonia nitrogen synchronize go
The autotrophic denitrification stable operation stage removed；
First stage cultivates water inlet for simulation sewage, it is desirable to SS is less than 100mg/L, and initial influent ammonia nitrogen is less than
100mg/L, COD concentration is less than 50mg/L, and device hydraulic detention time is 5-7 days, and preaeration district aeration uses water dissolution oxygen
Concentration controls as 2-3mg/L, when water outlet ammonia nitrogen removal frank is stabilized to 60～70%, promotes influent ammonia nitrogen to 100-200mg/L, when
Water outlet ammonia nitrogen removal frank is stabilized to 60-70%, enters second stage；
Second stage water inlet mixes with actual sewage 1:1 by volume for simulation sewage, it is desirable to SS is less than 100mg/L,
Ammonia nitrogen, COD concentration are respectively less than 100mg/L, more than pH7.5, and device hydraulic detention time is 3 days, and preaeration district aeration makes
Water outlet Dissolved Oxygen concentration Control is below 0.5mg/L, when ammonia nitrogen removal frank be more than 80%, nitrogen removal rate more than 60%, enter
Enter steady operation period, complete the startup of reaction unit；
Every liter described of simulation sewage contains: NH4Cl 0.1g, NaHCO30.2g, pH7.0-7.5, surplus is water.
Preaeration district function is pre-oxidation, controls water inlet dissolved oxygen；Inhalant region is then to remove water body by precipitation to suspend
Thing also retains macromolecule contaminant, according to water inlet concentration of suspension and former regimen condition, cleans every 1-3 month.Second lattice are for changing
Property zeolite filler district, primary attachment ammonia nitrogen and cation, the enrichment autotrophic denitrification micropopulation based on ammoxidation；3rd lattice are
Modified magnetic iron ore packing area, the enrichment autotrophic denitrification micropopulation based on Anammox, ammonia under main responsible anoxia condition
Oxidation is removed with total nitrogen partly.
After the present invention can be by simple cultivation mud alive, utilize packing property can accelerate the formation of autotrophic denitrification mud,
Further may select and mould different types of denitrifying sludge, the autotrophic denitrification microorganism being dominant with ammonia oxidation microbiological such as formation
Group, efficiently completes the work that mineralized nitrogen is nitrite, and forms the autotrophic denitrification being dominant with anaerobic ammonia oxidation microbiological
Micropopulation, efficiently completes the work that total nitrogen is removed, and therefore, the present invention designs corresponding filler and reaction dress according to its selectivity characteristic
Putting, orientation segmentation selects autotrophic denitrification microorganism so that it is carries out more efficient deposit efficiency on different functional stuffings, improves it
Activity, i.e. carries out selective enrichment to ammonia oxidation microbiological with anaerobic ammonia oxidation microbiological and separates, it is thus possible at micro-oxygen conditions
Ammonia nitrogen in lower removal natural water, municipal wastewater and total polluted by nitrogen.
Accompanying drawing illustrates:
Fig. 1 is the structure chart of the reaction unit of the present invention；
Wherein 1, preaeration district；2, inhalant region；3, modified zeolite packing area；4, modified magnetic iron ore packing area；5, water precipitating is gone out
Shallow lake district；6, water inlet；7, outlet；8, preaerator；
Fig. 2 is high-flux sequence modification magnetic iron ore packing area, modified zeolite packing area ammonia oxidation microbiological relative abundance (phase
To quantity ratio), the autotrophic denitrification mud of a-inoculation；ZE-modified zeolite packing area；MA-modification magnetic iron ore packing area；
Fig. 3 is high-flux sequence modification magnetic iron ore packing area, modified zeolite packing area ammonia oxidation microbiological relative abundance (phase
To quantity ratio), the autotrophic denitrification mud of a-inoculation；ZE-modified zeolite packing area；MA-modification magnetic iron ore packing area；
Fig. 4 is the ammoxidation activity in different filler enrichment sections, the autotrophic denitrification mud of a-inoculation；ZE-modified zeolite is filled out
Material district；MA-modification magnetic iron ore packing area.
Detailed description of the invention:
Following example are to further illustrate the present invention rather than limitation of the present invention.
Embodiment 1:
The magnetic iron ore of magnetic iron ore Fillers selection particle diameter 0.5cm, filler uses tap water to clean, until supernatant is clarified.So
Soak 20min with clear water afterwards, with the ultrasonic 30min of low frequency 40kHz, add volume ratio concentrated hydrochloric acid (mass fraction 37%): water=
The hydrochloric acid solution of 1:9 soaks 30min (adjusting pH is 3), finally rinses to pH=7 neutral with clear water, and 120 DEG C of drying 24h are stand-by,
Obtain modified magnetic iron ore filler.
Selecting the zeolite filler of particle diameter 0.5cm, filler uses tap water to clean, and then uses low frequency 40kHz ultrasonic
30min.Add volume ratio concentrated hydrochloric acid (mass fraction 37%): the hydrochloric acid solution of water=1:9 rinses extremely with clear water after soaking 20min
PH=7 is neutral.The NaOH immersion 30min, pH that add mass fraction 10% concentration adjust to 12, soak zeolite filler 12h.?
Rinsing to neutral (pH=7) with clear water afterwards, 120 DEG C of drying 24h are stand-by, obtain modified zeolite filler.
Taking the active sludge intermixture of the municipal wastewater treatment plant of 1000ml, its VSS (in sewage, forge through high temperature 575 DEG C by float
After burning, the organic concentration vapored away) concentration 2mg/L.After excluding impurity and precipitation, topple over the body such as supernatant addition
Long-pending M0 culture fluid, continuous aeration and stirring, control dissolved oxygen is 2-3mg/L, measures supernatant ammonia nitrogen concentration every 3 days.When
Ammonia nitrogen degradation efficiency reaches 92%, and nitrite accumulation rate reaches more than 50%, then changes equal-volume M0 culture fluid, continuous aeration
With stirring, control dissolved oxygen is 2-3mg/L, cultivates 2 weeks, measures supernatant ammonia nitrogen concentration.When ammonia nitrogen degradation efficiency reaches 95%
Above, nitrite accumulation rate reaches 80%, it is thus achieved that mud A；Half mud A is transferred to another one container, controls aeration,
Making its dissolved oxygen is 1mg/L, measures supernatant ammonia nitrogen concentration every 3 days, and ammonia nitrogen degradation efficiency reaches 70-80%, nitrite
Degradation rate is 100%, changes equal-volume M1 culture fluid, controls aeration so that it is dissolved oxygen is 1mg/L, after repeating to cultivate 3-4 week,
Supernatant ammonia nitrogen, nitrite concentration is measured every 3 days.When ammonia nitrogen degradation efficiency reaches 92%, and Nitrite accumulation disappears, and obtains
Obtain mud B (autotrophic denitrification mud).
As it is shown in figure 1, the reaction unit of the present embodiment includes preaeration district 1, inhalant region 2 that tandem is connected, changes
Property zeolite filler district 3, modified magnetic iron ore packing area 4 and water outlet settling zone 5, the front end in described preaeration district is provided with water inlet 6,
The tail end of described water outlet settling zone is provided with outlet 7, equipped with preaerator 8 in preaeration district, and described modified zeolite
Packing area is equipped with modified zeolite filler, and described modified magnetic iron ore packing area is equipped with modified magnetic iron ore filler, and water is by water inlet 6
Enter, flow through preaeration district 1, inhalant region 2, modified zeolite packing area 3, modified magnetic iron ore packing area 4 and water outlet settling zone 5, from going out
The mouth of a river 7 is flowed out.
On the modified zeolite filler being inoculated in reaction unit by mud B and modified magnetic iron ore filler, then carry out biofilm,
The continuous stream mode startup optimization of reaction unit employing again:
First stage cultivates and intakes as simulating sewage, and water inlet SS (solid suspension concentration), less than 100mg/L, initiates water inlet
Ammonia nitrogen is 50mg/L, COD concentration less than 50mg/L, and device hydraulic detention time is 5 days, room temperature, preaeration district, front end 1 pre-
It is 3mg/L that aerator 8 assists aeration to use the control of water dissolution oxygen concentration.When water outlet ammonia nitrogen removal frank is stabilized to 60-70%, carry
Rise influent ammonia nitrogen to 100mg/L, when water outlet ammonia nitrogen removal frank is stabilized to 70%, then improve influent ammonia nitrogen to 200mg/L, entrance the
Two-stage.
Second stage water inlet is the mixed liquor simulating sewage and actual sewage according to volume ratio 1:1, and its SS is less than 100mg/
L, ammonia nitrogen, COD concentration is respectively 100mg/L, 70mg/L, and pH is 7.5.Device hydraulic detention time shortens to 3 days, and room temperature is auxiliary
Help aeration to use water dissolution oxygen concentration to control as below 0.5mg/L.After measuring the 3rd week ammonia nitrogen removal frank be more than 80%, total nitrogen
Clearance more than 60%, enters steady operation period.
Every liter described of simulation sewage contains: NH4Cl 0.1g, NaHCO30.2g, pH7.0-7.5, surplus is tap water,
Its component is pressed its content mix homogeneously by its compound method.
Described M0 culture fluid every liter contains: NH4Cl 0.1g, NaHCO30.2g, surplus is water, its compound method be by
NH4Cl 0.1g、NaHCO30.2g is added to the water, and uses NaHCO3Solution adjust pH value to 7.0, then constant volume 1L.
Described M1 culture fluid every liter contains: NH4Cl 0.5g, NaNO20.5g, NaHCO30.5g, surplus is water, and it is joined
Method processed is by NH4Cl 0.5g, NaNO20.5g, NaHCO30.5g is added to the water, and uses NaHCO3Solution adjust pH value to 7.5,
Then constant volume 1L.
It is not filled with modified magnetic iron ore filler and modified zeolite filler with modified magnetic iron ore packing area and modified zeolite packing area,
Only filling autotrophic denitrification mud is as comparison.
After 1 month cultivates, Fig. 2 and Fig. 3 is shown by high throughput analysis, and modified magnetic iron ore packing area (is filled with and changes
Property magnetic iron ore filler, lower with) amount of the aerobic ammonia oxidation microorganism of average accumulated be height more than inoculation autotrophic denitrification mud, reach
10%, exceed more than 50% with inoculation autotrophic denitrification mud ratio, on the other hand in terms of the relative abundance of anaerobic ammonia oxidation microbiological
Most to the quantity of modified magnetic iron ore packing area accumulation, averagely reach 10%, and modified zeolite packing area (is filled with modified zeolite
Filler, lower same) it is then 0.08%, all exceed 50% than seed sludge.Find out (Fig. 4) by ammoxidation activity Experimental Comparison, change
Property magnetic iron ore packing area ammoxidation activity the strongest, (unit is: milligram nitrogen/every milligram averagely to reach 2mgN*h-1prtein*g-1
Albumen filler per hour per gram), and modified zeolite packing area is 1.48mgN*h-1prtein*g-1, the mud of inoculation only has
The activity of 50%；Nitrification activity is then the strongest in zeolite section, and magnetic iron ore is the most weak, shows that the different materials composition to microorganism is with active
Cause orthoselection.
Embodiment 2:
The magnetic iron ore of magnetic iron ore Fillers selection particle diameter 1cm, filler uses tap water to clean, until supernatant is clarified.Then
Soak 35min with clear water, with the ultrasonic 60min of low frequency 40kHz, add volume ratio concentrated hydrochloric acid (mass fraction 37%): water=1:9
Hydrochloric acid solution soak 20min (adjust pH be 3), finally rinse to pH=7 neutral with clear water, 120 DEG C to dry 24h stand-by,
To modified magnetic iron ore filler.
Selecting the zeolite filler of particle diameter 1cm, filler uses tap water to clean, and then uses the ultrasonic 60min of low frequency 40kHz.
Add volume ratio concentrated hydrochloric acid (mass fraction 37%): the hydrochloric acid solution of water=1:9 rinses to pH=7 with clear water after soaking 35min
Neutral.The NaOH immersion 30min, pH that add mass fraction 10% concentration adjust to 12, soak zeolite filler 12h.Finally use
Clear water rinses to neutral (pH=7), and 120 DEG C of drying 48h are stand-by, obtain modified zeolite filler.
Taking the municipal wastewater treatment plant active sludge intermixture of 2000ml, (in sewage, float is through high temperature 575 DEG C calcining for its VSS
After, the organic concentration vapored away) concentration 1.5mg/L.After excluding impurity and precipitation, topple over the body such as supernatant addition
Long-pending M0 culture fluid, continuous aeration and stirring, control dissolved oxygen is 2mg/L, measures supernatant ammonia nitrogen concentration every 4 days.Work as ammonia nitrogen
Degradation efficiency reaches 91%, and nitrite accumulation rate reaches 66%, then changes equal-volume M0 culture fluid, continuous aeration and stirring,
Control dissolved oxygen is 2mg/L, cultivates 2 weeks, measures supernatant ammonia nitrogen concentration.When ammonia nitrogen degradation efficiency reaches more than 95%, nitrous
Hydrochlorate cumulative percentage reaches 80%, it is thus achieved that mud A；Half mud A is transferred to another one container, controls aeration so that it is dissolve
Oxygen is 0.5mg/L, measures supernatant ammonia nitrogen concentration every 3 days, and ammonia nitrogen degradation efficiency reaches 70-80%, nitrite degradation rate
It is 100%, changes equal-volume M1 culture fluid, control aeration so that it is dissolved oxygen is 0.5mg/L, after repeating to cultivate 3-4 week, every 3
It measures supernatant ammonia nitrogen, nitrite concentration.When ammonia nitrogen degradation efficiency reaches 92%, and Nitrite accumulation disappears, it is thus achieved that dirty
Mud B (autotrophic denitrification mud).
Every liter described of simulation sewage contains: NH4Cl 0.1g, NaHCO30.2g, pH7.0-7.5, surplus is tap water,
Its component is pressed its content mix homogeneously by its compound method.
Described M0 culture fluid every liter contains: NH4Cl 0.1g, NaHCO30.2g, surplus is water, its compound method be by
NH4Cl 0.1g、NaHCO30.2g is added to the water, and uses NaHCO3Solution adjust pH value to 7.0, then constant volume 1L.
Described M1 culture fluid every liter contains: NH4Cl 0.5g, NaNO20.5g, NaHCO30.5g, surplus is water, and it is joined
Method processed is by NH4Cl 0.5g, NaNO20.5g, NaHCO30.5g is added to the water, and uses NaHCO3Solution adjust pH value to 7.5,
Then constant volume 1L.
As it is shown in figure 1, the reaction unit of the present embodiment includes preaeration district 1, inhalant region 2 that tandem is connected, changes
Property zeolite filler district 3, modified magnetic iron ore packing area 4 and water outlet settling zone 5, the front end in described preaeration district is provided with water inlet 6,
The tail end of described water outlet settling zone is provided with outlet 7, equipped with preaerator 8 in preaeration district, and described modified zeolite
Packing area is equipped with modified zeolite filler, and described modified magnetic iron ore packing area is equipped with modified magnetic iron ore filler, and water is by water inlet 6
Enter, flow through preaeration district 1, inhalant region 2, modified zeolite packing area 3, modified magnetic iron ore packing area 4 and water outlet settling zone 5, from going out
The mouth of a river 7 is flowed out.
On the modified zeolite filler being inoculated in reaction unit by mud B and modified magnetic iron ore filler, then carry out biofilm,
The continuous stream mode startup optimization of reaction unit employing again:
It is simulation sewage that first stage cultivates water inlet, and water inlet SS is less than 100mg/L, and initial influent ammonia nitrogen, COD are 50mg/
L, device hydraulic detention time is 7 days, and preaeration district auxiliary aeration is used water dissolution oxygen concentration and controlled as 2mg/L.When water outlet ammonia
Nitrogen removal efficiency is stabilized to 70%, promotes influent ammonia nitrogen to 100mg/L, when water outlet ammonia nitrogen removal frank is stabilized to 80%, then improve into
Water ammonia nitrogen is to 150mg/L, when water outlet ammonia nitrogen removal frank is stabilized to 60-70% time, enters second stage；
Second stage water inlet is the mixed liquor that simulation sewage mixes with actual sewage 1:1 by volume, controls SS and is less than
100mg/L, ammonia nitrogen, COD concentration is respectively 100mg/L, 50mg/L, and pH is 7.8.Device hydraulic detention time shortens to 5 days, often
Temperature, auxiliary aeration is used water dissolution oxygen concentration and is controlled as below 0.5mg/L.After measuring the 3rd week ammonia nitrogen removal frank be 80% with
Upper, nitrogen removal rate more than 60%, enters steady operation period.
Claims (4)
1. the method for the autotrophic denitrification micropopulation of an orienting enriching different proportion, it is characterised in that comprise the following steps:
A, the modification of filler
The modification of magnetic iron ore filler: after the Magnet mining water of particle diameter 0.5～1cm is cleaned up, more modified with soak with hydrochloric acid,
Rinse the magnetic iron ore after hydrochloric Acid Modification with water afterwards neutral to pH=7, dry, obtain modified magnetic iron ore filler；
The modification of zeolite filler: after the zeolite filler water of particle diameter 0.5-1cm is cleaned up, more modified with soak with hydrochloric acid,
Rinsing the zeolite filler after hydrochloric Acid Modification with water afterwards neutral to pH=7, then soak modification by NaOH solution, final rinse water is extremely
PH=7 is neutral, dries, obtains modified zeolite filler；
B, the preparation of autotrophic denitrification mud
Take the VSS concentration activated sludge more than 1mg/L, after excluding impurity, precipitation, then replace supernatant with M0 culture fluid,
Continuous aeration and stirring so that it is dissolved oxygen is 2-3mg/L, when ammonia nitrogen degradation efficiency reaches more than 90%, nitrite accumulation rate
When reaching more than 30%, change M0 culture fluid and continue to cultivate, continuous aeration and stirring so that it is dissolved oxygen is 2～3mg/L, until
Ammonia nitrogen degradation efficiency reaches more than 90%, and nitrite accumulation rate reaches more than 60%, it is thus achieved that mud A；
Taking mud A and move in another one container, aeration so that it is dissolved oxygen is 0.5～1mg/L, when ammonia nitrogen degradation efficiency reaches
When 70～80%, when nitrite degradation rate reaches more than 70%, change M1 culture fluid and continue to cultivate, continuous aeration and stirring,
Making its dissolved oxygen is 0.5～1mg/L, until ammonia nitrogen degradation efficiency reaches more than 90%, nitrite accumulation rate disappears, it is thus achieved that dirty
Mud B, is autotrophic denitrification mud；
Described M0 culture fluid every liter contains: NH4Cl 0.1g, NaHCO30.2g, surplus is water, pH7.0-7.5；
Described M1 culture fluid every liter contains: NH4Cl 0.5g, NaNO20.5g, NaHCO30.5g, surplus is water, pH7.5-
8.0；
The filling of C, reaction unit and filler
Reaction unit includes preaeration district that tandem is connected, inhalant region, modified zeolite packing area, modified magnetic iron ore filler
District and water outlet settling zone, the front end in described preaeration district is provided with water inlet, and the tail end of described water outlet settling zone is provided with water outlet
Mouthful, equipped with preaerator in preaeration district, described modified zeolite packing area is equipped with modified zeolite filler, described modification
Magnetic iron ore packing area is equipped with modified magnetic iron ore filler；
D, the startup of reaction unit and operation: the modified zeolite filler that mud B is inoculated in reaction unit and modified magnetic iron ore
On filler, then carry out biofilm, then reaction unit uses continuous stream mode startup optimization.
Method the most according to claim 1, it is characterised in that the modification of described magnetic iron ore filler be by particle diameter 0.5～
The magnetic iron ore of 1cm washes with water, until supernatant is clarified, is then soaked in water 20～35min, then with the ultrasonic 30-60min of 40kHz,
Adding hydrochloric acid to pH is 3, then soaks 20-30min, and final rinse water to pH=7 is neutral, and 120 DEG C of drying obtain modification
Magnetic iron ore filler.
Method the most according to claim 1, it is characterised in that the modification of described zeolite filler is by particle diameter 0.5-1cm
Zeolite filler use water clean, then use the ultrasonic 30-60min of 40kHz, add and rinse with water after hydrochloric acid soaks 20-35min
Neutral to pH=7, the NaOH solution immersion 30min, pH that add mass fraction 10% adjust to more than 12, soak zeolite filler
12h, final rinse water to neutral pH=7,120 DEG C of drying, obtain modified zeolite filler.
Method the most according to claim 1, it is characterised in that described reaction unit uses continuous stream mode to start fortune
OK, it concretely comprises the following steps:
Reaction unit is divided into two stages, and the first stage, second stage was total nitrogen, ammonia nitrogen synchronization removal in order to remove the ammonia nitrogen stage
Autotrophic denitrification stable operation stage；
First stage cultivates water inlet for simulation sewage, it is desirable to SS is less than 100mg/L, initial influent ammonia nitrogen less than 100mg/L,
COD concentration is less than 50mg/L, and device hydraulic detention time is 5-7 days, and preaeration district aeration is used water dissolution oxygen concentration and controlled
For 2-3mg/L, when water outlet ammonia nitrogen removal frank is stabilized to 60～70%, promote influent ammonia nitrogen to 100-200mg/L, when water outlet ammonia nitrogen
Clearance is stabilized to 60-70%, enters second stage；
Second stage water inlet mixes with actual sewage 1:1 by volume for simulation sewage, it is desirable to SS less than 100mg/L, ammonia nitrogen,
COD concentration is respectively less than 100mg/L, more than pH7.5, and device hydraulic detention time is 3 days, and preaeration district aeration is used water-soluble
Solve oxygen concentration to control as below 0.5mg/L, when ammonia nitrogen removal frank be more than 80%, nitrogen removal rate more than 60%, enter stable
Runtime, complete the startup of reaction unit；
Every liter of sewage of simulation contains: NH4Cl 0.1g, NaHCO30.2g, pH7.0-7.5, surplus is water.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107185505A (en) * | 2017-06-12 | 2017-09-22 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of modified zeolite polyurethane compounded mix and its method of modifying and application |
| CN107226600A (en) * | 2017-06-16 | 2017-10-03 | 广州泰通环保科技投资有限公司 | River bottom mud repair system |
| CN108611276A (en) * | 2018-04-16 | 2018-10-02 | 仲恺农业工程学院 | A method of recycling treatment being carried out to distillery waste water using microalgae |
| CN114230021A (en) * | 2021-12-17 | 2022-03-25 | 南京大学 | Biological composite filler and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020015606A (en) * | 2000-08-22 | 2002-02-28 | 김흥기 | complex wastewater treatment device using 3 level zeolite filter |
| CN203269716U (en) * | 2012-12-24 | 2013-11-06 | 深圳市深港产学研环保工程技术股份有限公司 | Artificial rapid infiltration system for industrial park sewage treatment |
-
2016
- 2016-08-30 CN CN201610771705.2A patent/CN106282020B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020015606A (en) * | 2000-08-22 | 2002-02-28 | 김흥기 | complex wastewater treatment device using 3 level zeolite filter |
| CN203269716U (en) * | 2012-12-24 | 2013-11-06 | 深圳市深港产学研环保工程技术股份有限公司 | Artificial rapid infiltration system for industrial park sewage treatment |
Non-Patent Citations (4)
| Title |
|---|
| SUQING WU等: "Preparation of ceramic filler from reusing sewage sludge and application in biological aerated filter for soy protein secondary wastewater treatment", 《JOURNAL OF HAZARDOUS MATERIALS》 * |
| 耿淑英等: "SBＲ 系统外加磁场对微生物群落多样性和处理效果的影响", 《环境科学》 * |
| 郭小马等: "分格复合填料曝气生物滤池脱氮除磷特性及微生物", 《环境科学学报》 * |
| 钟玉鸣: "浅谈SBR污水处理技术的进展", 《广州环境科学》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107185505A (en) * | 2017-06-12 | 2017-09-22 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of modified zeolite polyurethane compounded mix and its method of modifying and application |
| CN107185505B (en) * | 2017-06-12 | 2020-09-08 | 广东省微生物研究所（广东省微生物分析检测中心） | Modified zeolite-polyurethane composite filler and modification method and application thereof |
| CN107226600A (en) * | 2017-06-16 | 2017-10-03 | 广州泰通环保科技投资有限公司 | River bottom mud repair system |
| CN108611276A (en) * | 2018-04-16 | 2018-10-02 | 仲恺农业工程学院 | A method of recycling treatment being carried out to distillery waste water using microalgae |
| CN114230021A (en) * | 2021-12-17 | 2022-03-25 | 南京大学 | Biological composite filler and preparation method and application thereof |
| CN114230021B (en) * | 2021-12-17 | 2023-03-07 | 南京大学 | Biological composite filler and preparation method and application thereof |
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