CN106282020B - A kind of method of the autotrophic denitrification micropopulation of orienting enriching different proportion - Google Patents
A kind of method of the autotrophic denitrification micropopulation of orienting enriching different proportion Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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Abstract
The invention discloses a kind of methods of the autotrophic denitrification micropopulation of orienting enriching different proportion.After the present invention can be by simply cultivating sludge living, it can accelerate the formation of autotrophic denitrification sludge using packing property, further it may be selected to mould different types of denitrifying sludge, as formed the autotrophic denitrification micropopulation being dominant with ammonia oxidation microbiological, efficiently complete the work that mineralized nitrogen is nitrite, and form the autotrophic denitrification micropopulation being dominant with anaerobic ammonia oxidation microbiological, efficiently complete the work of total nitrogen removal, therefore, the present invention selects characteristic to design corresponding filler and reaction unit according to it, orientation segmentation selection autotrophic denitrification microorganism, it is set to carry out more efficient deposit efficiency on different functional stuffings, improve its activity, i.e. to ammonia oxidation microbiological and anaerobic ammonia oxidation microbiological carry out selective enrichment with separate, so as to remove natural water under micro-oxygen conditions, ammonia nitrogen and total polluted by nitrogen in municipal wastewater.
Description
Technical field
The invention belongs to water process and environmental technology field, and in particular to one kind is with magnetic iron ore, modified zeolite orienting enriching
The method of autotrophic denitrification microorganism.
Background technique
Currently, the water environment pollution problem in China receives significant attention, ammonia nitrogen, total nitrogen, organic matter and novel toxic are dirty
The problem of contaminating object is emerged one after another.The control of total nitrogen total emission volumn is implemented to coastal ground level and the above city in national new " ten, water "
System requires, so that the requirement to water process improves a higher level.
For accounting for pollutant-ammonia nitrogen of 60% or more water body total nitrogen, from the degradation of all kinds of nitrogen-containing pollutants
Journey.In general, ammonia nitrogen only converts under the action of ammonia oxidation microbiological, transformation time is related to dissolved oxygen concentration,
Its accumulation often causes further anoxic in polluted-water, and anoxic has the accumulation for further resulting in ammonia nitrogen.In addition, ammonia nitrogen is water
Nutrient in body can lead to water eutrophication, while to the toxic property of fish and many aquatiles.Therefore, people
Improvement for ammonia and nitrogen pollution conducts extensive research.After removing ammonia nitrogen using the method for excess aeration, lead to a large amount of ammonia
Nitrogen is converted into nitrate, and the problem for keeping water body total nitrogen excessively high starts to emerge.Water body denitrification mainly passes through with trace contaminant removal
The metabolic activity of microorganism is completed.The removal process of traditional microbiological total nitrogen needs to carry out denitrification again by ammoxidation, nitrification
Process.It is nitrate that this process, which needs a large amount of be aerated mineralized nitrogen, then removes nitre using denitrification under organic conditions
Hydrochlorate.It completely removes and needs to consume a large amount of electric energy and carbon source (methanol).The ammonia and nitrogen pollution got worse is handled, has allowed and has unbearably weighed
Negative water treatment system energy consumption increases severely, and the appearance of emerging pollutant, and the pressure of processing will be further aggravated.Currently, novel
Denitrogenation technology is always the forward position of environmental project research.Autotrophic denitrification technique is exactly that one of which takes off with energy-efficient very much
Nitrogen technology.Autotrophic denitrification technology can remove ammonia nitrogen and total nitrogen under conditions of lacking organic carbon source, greatly save power energy consumption with
Carbon source consumption, therefore be a kind of very promising denitrification process.Therefore, it develops one kind and is directly applied for cell or country sewage
Pretreatment, can remove acetaminophen but also simultaneous removing total nitrogen, ammonia nitrogen novel energy-saving environment-friendly water treatment system, not only favorably
Dirty cities and towns Water Pollution Problem can not be cut in alleviating to improve, it helps the safety for improving water environment, to the following 3-5 distributing
The development of water treatment technology has great importance.
However, starting autotrophic denitrification micropopulation needs phase since the doubling time of autotrophic denitrification micropopulation is considerably long
When the long time, it is allowed to be restricted in engineer application.How to accelerate its incubation is the key that practical application.
Summary of the invention
Natural water, the ammonia nitrogen in municipal wastewater and total can be removed under micro-oxygen conditions the object of the present invention is to provide a kind of
The method of the autotrophic denitrification micropopulation of the orienting enriching different proportion of polluted by nitrogen.
The method of the autotrophic denitrification micropopulation of orienting enriching different proportion of the invention, which is characterized in that including following
Step:
A, the modification of filler
The modification of magnetic iron ore filler: by the magnetic iron ore of 0.5~1cm of partial size wash with water it is clean after, then changed with salt acid soak
Property, for the magnetic iron ore after final rinse water hydrochloric Acid Modification to pH=7 neutrality, drying obtains modified magnetic iron ore filler；
The modification of zeolite filler: by the zeolite filler of partial size 0.5-1cm wash with water it is clean after, then changed with salt acid soak
Property, zeolite filler after final rinse water hydrochloric Acid Modification to pH=7 neutrality, then impregnated and be modified with NaOH solution, finally use water
It rinses to pH=7 neutrality, drying obtains modified zeolite filler；
B, the preparation of autotrophic denitrification sludge
VSS concentration is taken to be greater than the activated sludge of 1mg/L, after excluding impurity, precipitating, then with M0 culture solution displacement supernatant
Liquid, continuous aeration and stirring, making it dissolve oxygen is 2-3mg/L, when ammonia nitrogen degradation efficiency reaches 90% or more, Nitrite accumulation
When rate reaches 30% or more, replacement M0 culture solution continues to cultivate, continuous aeration and stirring, and making it dissolve oxygen is 2~3mg/L, directly
Reach 90% or more to ammonia nitrogen degradation efficiency, nitrite accumulation rate reaches 60% or more, obtains sludge A；
It takes sludge A to move in another container, is aerated, making it dissolve oxygen is 0.5~1mg/L, when ammonia nitrogen degradation efficiency
When reaching 70~80%, when nitrite degradation rate reaches 70% or more, replacement M1 culture solution continues to cultivate, and continuous aeration and stirs
It mixes, making it dissolve oxygen is 0.5~1mg/L, until ammonia nitrogen degradation efficiency reaches 90% or more, nitrite accumulation rate disappears, and is obtained
Obtain sludge B, as autotrophic denitrification sludge；
Every liter of the M0 culture solution contains: NH4Cl 0.1g, NaHCO30.2g, surplus are water, pH7.0-7.5；
Every liter of the M1 culture solution contains: NH4Cl 0.5g, NaNO20.5g, NaHCO30.5g, surplus are water,
pH7.5-8.0；
C, the filling of reaction unit and filler
Reaction unit includes preaeration area, inhalant region, the modified zeolite packing area, modified magnetic iron ore that tandem is connected
Packing area and water outlet settling zone, the front end in the preaeration area are equipped with water inlet, and the tail end of the water outlet settling zone is equipped with
Water outlet is equipped with preaerator in preaeration area, and the modified zeolite packing area is equipped with modified zeolite filler, described
Modified magnetic iron ore packing area is equipped with modified magnetic iron ore filler；
D, reaction unit is started and run: the modified zeolite filler and modified magnetic sludge B being inoculated into reaction unit
On iron ore filler, biofilm, then reaction unit are then carried out using continuous stream mode starting operation.
The modification of the magnetic iron ore filler is that the magnetic iron ore of 0.5~1cm of partial size is washed with water, until supernatant clarify,
Then be soaked in water 20~35min, then with 40kHz ultrasound 30-60min, and adding hydrochloric acid to pH is 3, then impregnates 20-
30min, final rinse water to pH=7 neutrality, 120 DEG C of drying obtain modified magnetic iron ore filler；
The modification of the zeolite filler is to wash with water the zeolite filler of partial size 0.5-1cm, then super using 40kHz
Sound 30-60min is added in hydrochloric acid after impregnating 20-35min and is rinsed with water to pH=7 neutrality, adds mass fraction 10%
NaOH solution impregnates 30min, and pH is adjusted to 12 or more, impregnates zeolite filler 12h, final rinse water to neutral pH=7,120
DEG C drying, obtain modified zeolite filler.
The reaction unit is run using continuous stream mode starting, the specific steps are that:
Reaction unit is divided into two stages, and the first stage is the removal ammonia nitrogen stage, and second stage is total nitrogen, ammonia nitrogen synchronizes
The autotrophic denitrification stable operation stage removed；
First stage culture water inlet is simulation sewage, it is desirable that SS is no more than 100mg/L, and starting influent ammonia nitrogen is no more than
100mg/L, COD concentration are no more than 50mg/L, and device hydraulic detention time is 5-7 days, and preaeration area aeration uses water dissolved oxygen
Concentration control is 2-3mg/L, when water outlet ammonia nitrogen removal frank is stabilized to 60~70%, promotes influent ammonia nitrogen to 100-200mg/L, when
Water outlet ammonia nitrogen removal frank is stabilized to 60-70%, into second stage；
For simulation sewage, 1:1 is mixed by volume with actual sewage for second stage water inlet, it is desirable that and SS is no more than 100mg/L,
Ammonia nitrogen, COD concentration are no more than 100mg/L, pH7.5 or more respectively, and device hydraulic detention time is 3 days, and preaeration area aeration makes
Water outlet Dissolved Oxygen concentration Control is 0.5mg/L hereinafter, when ammonia nitrogen removal frank is 80% or more, 60% or more nitrogen removal rate, into
Enter steady operation period, completes the starting of reaction unit；
Every liter of the simulation sewage contains: NH4Cl 0.1g, NaHCO30.2g, pH7.0-7.5, surplus are water.
Preaeration area function is to pre-oxidize, control water inlet dissolved oxygen；Inhalant region is suspended by precipitating removal water body
Object simultaneously retains macromolecule contaminant, according to water inlet concentration of suspension and raw water situation, every cleaning in 1-3 months.Second lattice are to change
Property zeolite filler area, primary attachment ammonia nitrogen and cation are enriched with autotrophic denitrification micropopulation based on ammoxidation；Third lattice are
Modified magnetic iron ore packing area, is enriched with the autotrophic denitrification micropopulation based on Anammox, is mainly responsible for ammonia under anoxia condition
Oxidation and the total nitrogen of part remove.
After the present invention can be by simply cultivating sludge living, the formation of autotrophic denitrification sludge can be accelerated using packing property,
It further may be selected to mould different types of denitrifying sludge, such as form the autotrophic denitrification microorganism being dominant with ammonia oxidation microbiological
Group is efficiently completed the work that mineralized nitrogen is nitrite, and forms the autotrophic denitrification being dominant with anaerobic ammonia oxidation microbiological
Micropopulation efficiently completes the work of total nitrogen removal, and therefore, the present invention selects characteristic to design corresponding filler to fill with reacting according to it
It sets, orientation segmentation selection autotrophic denitrification microorganism makes it carry out more efficient deposit efficiency on different functional stuffings, improves it
Activity, i.e., to ammonia oxidation microbiological and anaerobic ammonia oxidation microbiological carry out selective enrichment with separate, so as in micro-oxygen conditions
Lower removal natural water, ammonia nitrogen and total polluted by nitrogen in municipal wastewater.
Detailed description of the invention:
Fig. 1 is the structure chart of reaction unit of the invention；
Wherein 1, preaeration area；2, inhalant region；3, modified zeolite packing area；4, modified magnetic iron ore packing area；5, water outlet is heavy
Shallow lake area；6, water inlet；7, water outlet；8, preaerator；
Fig. 2 is high-flux sequence modified magnetic iron ore packing area, modified zeolite packing area ammonia oxidation microbiological relative abundance (phase
To quantity ratio), the autotrophic denitrification sludge of a- inoculation；ZE- modified zeolite packing area；MA- is modified magnetic iron ore packing area；
Fig. 3 is high-flux sequence modified magnetic iron ore packing area, modified zeolite packing area ammonia oxidation microbiological relative abundance (phase
To quantity ratio), the autotrophic denitrification sludge of a- inoculation；ZE- modified zeolite packing area；MA- is modified magnetic iron ore packing area；
Fig. 4 is the ammoxidation activity in different fillers enrichment section, the autotrophic denitrification sludge of a- inoculation；ZE- modified zeolite is filled out
Expect area；MA- is modified magnetic iron ore packing area.
Specific embodiment:
The following examples are further illustrations of the invention, rather than limiting the invention.
Embodiment 1:
The magnetic iron ore of magnetic iron ore Fillers selection partial size 0.5cm, filler are cleaned using tap water, until supernatant is clarified.So
20min is impregnated with clear water afterwards to be added volume ratio concentrated hydrochloric acid (mass fraction 37%) with low frequency 40kHz ultrasound 30min: water=
The hydrochloric acid solution of 1:9 impregnates 30min (adjustment pH is 3), is finally rinsed with clear water to pH=7 neutrality, and 120 DEG C of drying are stand-by for 24 hours,
Obtain modified magnetic iron ore filler.
The zeolite filler of partial size 0.5cm is selected, filler is cleaned using tap water, then using low frequency 40kHz ultrasound
30min.Be added volume ratio concentrated hydrochloric acid (mass fraction 37%): water=1:9 hydrochloric acid solution impregnate 20min after with clear water rinse to
PH=7 is neutral.The NaOH for adding 10% concentration of mass fraction impregnates 30min, and pH is adjusted to 12, impregnates zeolite filler 12h.Most
It is rinsed afterwards with clear water to neutral (pH=7), 120 DEG C of drying are stand-by for 24 hours, obtain modified zeolite filler.
The active sludge intermixture of the municipal wastewater treatment plant of 1000ml is taken, VSS (forge through 575 DEG C of high temperature in sewage by suspended matter
After burning, the concentration of the organic matter vapored away) concentration 2mg/L.After excluding impurity and precipitating, the bodies such as supernatant and addition are toppled over
Long-pending M0 culture solution, continuous aeration and stirring, control dissolved oxygen was 2-3mg/L, every 3 days measurement supernatant ammonia nitrogen concentrations.When
Ammonia nitrogen degradation efficiency reaches 92%, and nitrite accumulation rate reaches 50% or more, then replaces isometric M0 culture solution, continuous aeration
With stirring, control dissolved oxygen is 2-3mg/L, cultivates 2 weeks, measures supernatant ammonia nitrogen concentration.When ammonia nitrogen degradation efficiency reaches 95%
More than, nitrite accumulation rate reaches 80%, obtains sludge A；Half sludge A is transferred to another container, control is aerated,
Making it dissolve oxygen is 1mg/L, and every 3 days measurement supernatant ammonia nitrogen concentrations, ammonia nitrogen degradation efficiency reached 70-80%, nitrite
Degradation rate is 100%, replaces isometric M1 culture solution, and control aeration, making it dissolve oxygen is 1mg/L, after repeating culture 3-4 weeks,
Every 3 days measurement supernatant ammonia nitrogens, nitrite concentrations.When ammonia nitrogen degradation efficiency reaches 92%, Nitrite accumulation disappears, and obtains
It obtains sludge B (autotrophic denitrification sludge).
As shown in Figure 1, the reaction unit of the present embodiment include tandem be connected preaeration area 1, inhalant region 2, change
Property zeolite filler area 3, modified magnetic iron ore packing area 4 and water outlet settling zone 5, the front end in the preaeration area is equipped with water inlet 6,
The tail end of the water outlet settling zone is equipped with water outlet 7, and preaerator 8, the modified zeolite are housed in preaeration area
Packing area is equipped with modified zeolite filler, and the modification magnetic iron ore packing area is equipped with modified magnetic iron ore filler, and water is by water inlet 6
Into preaeration area 1, inhalant region 2, modified zeolite packing area 3, modified magnetic iron ore packing area 4 and water outlet settling zone 5 being flowed through, from going out
It flows out at the mouth of a river 7.
On the modified zeolite filler and modified magnetic iron ore filler that sludge B is inoculated into reaction unit, biofilm is then carried out,
Reaction unit is using continuous stream mode starting operation again:
First stage culture water inlet is simulation sewage, and water inlet SS (solid suspension concentration) is less than 100mg/L, starting water inlet
Ammonia nitrogen be 50mg/L, COD concentration be no more than 50mg/L, device hydraulic detention time be 5 days, room temperature, front end preaeration area 1 it is pre-
It is 3mg/L that the auxiliary aeration of aerator 8, which uses water Dissolved Oxygen concentration Control,.It is stabilized to 60-70% when being discharged ammonia nitrogen removal frank, is mentioned
It rises influent ammonia nitrogen and arrives 100mg/L, be stabilized to 70% when being discharged ammonia nitrogen removal frank, then improve influent ammonia nitrogen to 200mg/L, into the
Two-stage.
Second stage water inlet is to simulate sewage and actual sewage according to the mixed liquor of volume ratio 1:1, and SS is less than 100mg/
L, ammonia nitrogen, COD concentration are respectively 100mg/L, 70mg/L, pH 7.5.Device hydraulic detention time is shorten to 3 days, and room temperature is auxiliary
Helping aeration to use water Dissolved Oxygen concentration Control is 0.5mg/L or less.Ammonia nitrogen removal frank is 80% or more, total nitrogen after three weeks for measurement the
60% or more removal rate, into steady operation period.
Every liter of the simulation sewage contains: NH4Cl 0.1g, NaHCO30.2g, pH7.0-7.5, surplus are tap water,
Its component is uniformly mixed by its preparation method by its content.
Every liter of the M0 culture solution contains: NH4Cl 0.1g, NaHCO30.2g, surplus are water, preparation method be by
NH4Cl 0.1g、NaHCO30.2g is added to the water, and uses NaHCO3Solution tune pH value is to 7.0, then constant volume 1L.
Every liter of the M1 culture solution contains: NH4Cl 0.5g, NaNO20.5g, NaHCO30.5g, surplus are water, are matched
Method processed is by NH4Cl 0.5g, NaNO20.5g, NaHCO30.5g is added to the water, and uses NaHCO3Solution tune pH value to 7.5,
Then constant volume 1L.
It is not filled with modified magnetic iron ore filler and modified zeolite filler to be modified magnetic iron ore packing area and modified zeolite packing area,
Only filling autotrophic denitrification sludge is as control.
After culture in 1 month, Fig. 2 and Fig. 3 are shown by high throughput analysis, and modified magnetic iron ore packing area (is filled with and changes
Property magnetic iron ore filler, similarly hereinafter) amount of the aerobic ammonia oxidation microorganism of average accumulated more than inoculation autotrophic denitrification sludge is height, reach
10%, it is higher by 50% or more with inoculation autotrophic denitrification sludge ratio, on the other hand in terms of the relative abundance of anaerobic ammonia oxidation microbiological
Quantity to modified magnetic iron ore packing area accumulation is most, averagely reaches 10%, and modified zeolite packing area (is filled with modified zeolite
Filler, similarly hereinafter) it is then 0.08%, 50% is higher by than seed sludge.(Fig. 4) is found out by ammoxidation activity Experimental Comparison, is changed
Property magnetic iron ore packing area ammoxidation activity is most strong, averagely reaches 2mgN*h-1prtein*g-1 (unit are as follows: milligram nitrogen/every milligram
Albumen filler per hour per gram), and modified zeolite packing area is 1.48mgN*h-1prtein*g-1, the sludge of inoculation only has
50% activity；Nitrification activity is then most strong in zeolite section, and magnetic iron ore is most weak, composition and activity of the display different materials to microorganism
Cause M8003 line.
Embodiment 2:
The magnetic iron ore of magnetic iron ore Fillers selection partial size 1cm, filler are cleaned using tap water, until supernatant is clarified.Then
35min, which is impregnated, with clear water adds volume ratio concentrated hydrochloric acid (mass fraction 37%): water=1:9 with low frequency 40kHz ultrasound 60min
Hydrochloric acid solution impregnate 20min (adjustment pH be 3), finally rinsed with clear water to pH=7 neutrality, 120 DEG C of drying are stand-by for 24 hours, obtain
To modified magnetic iron ore filler.
The zeolite filler of partial size 1cm is selected, filler is cleaned using tap water, then uses low frequency 40kHz ultrasound 60min.
Be added volume ratio concentrated hydrochloric acid (mass fraction 37%): water=1:9 hydrochloric acid solution is rinsed with clear water to pH=7 after impregnating 35min
It is neutral.The NaOH for adding 10% concentration of mass fraction impregnates 30min, and pH is adjusted to 12, impregnates zeolite filler 12h.Finally use
Clear water is rinsed to neutral (pH=7), and 120 DEG C of drying 48h are stand-by, obtain modified zeolite filler.
The municipal wastewater treatment plant active sludge intermixture of 2000ml is taken, VSS (calcine through 575 DEG C of high temperature in sewage by suspended matter
Afterwards, the concentration of the organic matter vapored away) concentration 1.5mg/L.After excluding impurity and precipitating, the bodies such as supernatant and addition are toppled over
Product M0 culture solution, continuous aeration and stirring, control dissolved oxygen was 2mg/L, every 4 days measurement supernatant ammonia nitrogen concentrations.Work as ammonia nitrogen
Degradation efficiency reaches 91%, and nitrite accumulation rate reaches 66%, then replaces isometric M0 culture solution, continuous aeration and stirring,
Control dissolved oxygen is 2mg/L, is cultivated 2 weeks, and supernatant ammonia nitrogen concentration is measured.When ammonia nitrogen degradation efficiency reaches 95% or more, nitrous
Hydrochlorate cumulative percentage reaches 80%, obtains sludge A；Half sludge A is transferred to another container, control aeration makes it dissolve
Oxygen is 0.5mg/L, and every 3 days measurement supernatant ammonia nitrogen concentrations, ammonia nitrogen degradation efficiency reached 70-80%, nitrite degradation rate
It is 100%, replaces isometric M1 culture solution, control aeration, making it dissolve oxygen is 0.5mg/L, after repeating culture 3-4 weeks, every 3
Its measurement supernatant ammonia nitrogen, nitrite concentration.When ammonia nitrogen degradation efficiency reaches 92%, Nitrite accumulation disappears, and obtains dirty
Mud B (autotrophic denitrification sludge).
Every liter of the simulation sewage contains: NH4Cl 0.1g, NaHCO30.2g, pH7.0-7.5, surplus are tap water,
Its component is uniformly mixed by its preparation method by its content.
Every liter of the M0 culture solution contains: NH4Cl 0.1g, NaHCO30.2g, surplus are water, preparation method be by
NH4Cl 0.1g、NaHCO30.2g is added to the water, and uses NaHCO3Solution tune pH value is to 7.0, then constant volume 1L.
Every liter of the M1 culture solution contains: NH4Cl 0.5g, NaNO20.5g, NaHCO30.5g, surplus are water, are matched
Method processed is by NH4Cl 0.5g, NaNO20.5g, NaHCO30.5g is added to the water, and uses NaHCO3Solution tune pH value to 7.5,
Then constant volume 1L.
As shown in Figure 1, the reaction unit of the present embodiment include tandem be connected preaeration area 1, inhalant region 2, change
Property zeolite filler area 3, modified magnetic iron ore packing area 4 and water outlet settling zone 5, the front end in the preaeration area is equipped with water inlet 6,
The tail end of the water outlet settling zone is equipped with water outlet 7, and preaerator 8, the modified zeolite are housed in preaeration area
Packing area is equipped with modified zeolite filler, and the modification magnetic iron ore packing area is equipped with modified magnetic iron ore filler, and water is by water inlet 6
Into preaeration area 1, inhalant region 2, modified zeolite packing area 3, modified magnetic iron ore packing area 4 and water outlet settling zone 5 being flowed through, from going out
It flows out at the mouth of a river 7.
On the modified zeolite filler and modified magnetic iron ore filler that sludge B is inoculated into reaction unit, biofilm is then carried out,
Reaction unit is using continuous stream mode starting operation again:
First stage culture water inlet is simulation sewage, and water inlet SS is less than 100mg/L, originates influent ammonia nitrogen, COD 50mg/
L, device hydraulic detention time are 7 days, and it is 2mg/L that preaeration area auxiliary aeration, which uses water Dissolved Oxygen concentration Control,.When water outlet ammonia
Nitrogen removal efficiency is stabilized to 70%, promotes influent ammonia nitrogen and arrives 100mg/L, is stabilized to 80% when being discharged ammonia nitrogen removal frank, then improve into
Water ammonia nitrogen is to 150mg/L, when water outlet ammonia nitrogen removal frank is stabilized to 60-70%, into second stage；
Second stage water inlet is simulation sewage and the actual sewage mixed liquor that 1:1 is mixed by volume, controls SS and is less than
100mg/L, ammonia nitrogen, COD concentration are respectively 100mg/L, 50mg/L, pH 7.8.Device hydraulic detention time shorten to 5 days, often
Temperature, it is 0.5mg/L or less that auxiliary aeration, which uses water Dissolved Oxygen concentration Control,.Measurement the after three weeks ammonia nitrogen removal frank be 80% with
Upper, 60% or more nitrogen removal rate, into steady operation period.
Claims (2)
1. a kind of method of the autotrophic denitrification micropopulation of orienting enriching different proportion, which comprises the following steps:
A, the modification of filler
The modification of magnetic iron ore filler is that the magnetic iron ore of 0.5~1cm of partial size is washed with water, and until supernatant clarification, then uses water logging
Steep 20~35min, then use 40kHz ultrasound 30-60min, add hydrochloric acid to pH be 3, then impregnate 20-30min, finally use water
It rinses to pH=7 neutrality, 120 DEG C of drying obtain modified magnetic iron ore filler；
The modification of zeolite filler is to wash with water the zeolite filler of partial size 0.5-1cm, then uses 40kHz ultrasound 30-
60min is added in hydrochloric acid after impregnating 20-35min and is rinsed with water to pH=7 neutrality, and the NaOH for adding mass fraction 10% is molten
Liquid impregnates 30min, and pH is adjusted to 12 or more, impregnates zeolite filler 12h, and final rinse water to neutral pH=7,120 DEG C is dried,
Obtain modified zeolite filler；
B, the preparation of autotrophic denitrification sludge
VSS concentration is taken to be greater than the activated sludge of 1mg/L, after excluding impurity, precipitating, then with M0 culture solution displacement supernatant,
Continuous aeration and stirring, making it dissolve oxygen is 2-3mg/L, when ammonia nitrogen degradation efficiency reaches 90% or more, nitrite accumulation rate
When reaching 30% or more, replacement M0 culture solution continues to cultivate, continuous aeration and stirring, and making it dissolve oxygen is 2~3mg/L, until
Ammonia nitrogen degradation efficiency reaches 90% or more, and nitrite accumulation rate reaches 60% or more, obtains sludge A；
It takes sludge A to move in another container, is aerated, making it dissolve oxygen is 0.5~1mg/L, when ammonia nitrogen degradation efficiency reaches
When 70~80%, when nitrite degradation rate reaches 70% or more, replacement M1 culture solution continues to cultivate, continuous aeration and stirring,
Making it dissolve oxygen is 0.5~1mg/L, until ammonia nitrogen degradation efficiency reaches 90% or more, nitrite accumulation rate disappears, and is obtained dirty
Mud B, as autotrophic denitrification sludge；
Every liter of the M0 culture solution contains: NH4Cl 0.1g, NaHCO30.2g, surplus are water, pH7.0-7.5；
Every liter of the M1 culture solution contains: NH4Cl 0.5g, NaNO20.5g, NaHCO30.5g, surplus are water, pH7.5-
8.0；
C, the filling of reaction unit and filler
Reaction unit includes preaeration area, inhalant region, the modified zeolite packing area, modified magnetic iron ore filler that tandem is connected
Area and water outlet settling zone, the front end in the preaeration area are equipped with water inlet, and the tail end of the water outlet settling zone is equipped with water outlet
Mouthful, preaerator is housed in preaeration area, the modified zeolite packing area is equipped with modified zeolite filler, the modification
Magnetic iron ore packing area is equipped with modified magnetic iron ore filler；
D, reaction unit is started and run: the modified zeolite filler and modified magnetic iron ore sludge B being inoculated into reaction unit
On filler, biofilm, then reaction unit are then carried out using continuous stream mode starting operation；
Modified zeolite packing area, absorption ammonia nitrogen and cation, are enriched with the autotrophic denitrification micropopulation based on ammoxidation；Modified magnetic
Iron ore packing area is enriched with the autotrophic denitrification micropopulation based on Anammox, is responsible for ammoxidation and part under anoxia condition
Total nitrogen removal.
2. the method according to claim 1, wherein the reaction unit is using continuous stream mode starting fortune
Row,
The specific steps are that:
Reaction unit is divided into two stages, and the first stage is the removal ammonia nitrogen stage, and second stage is total nitrogen, the synchronous removal of ammonia nitrogen
Autotrophic denitrification stable operation stage；
First stage culture water inlet for simulation sewage, it is desirable that SS be no more than 100mg/L, starting influent ammonia nitrogen be no more than 100mg/L,
COD concentration is no more than 50mg/L, and device hydraulic detention time is 5-7 days, and preaeration area aeration uses water Dissolved Oxygen concentration Control
For 2-3mg/L, 60~70% are stabilized to when being discharged ammonia nitrogen removal frank, promotes influent ammonia nitrogen to 100-200mg/L, when water outlet ammonia nitrogen
Removal rate is stabilized to 60-70%, into second stage；
For simulation sewage, 1:1 is mixed by volume with actual sewage for second stage water inlet, it is desirable that SS no more than 100mg/L, ammonia nitrogen,
COD concentration is no more than 100mg/L, pH7.5 or more respectively, and device hydraulic detention time is 3 days, and preaeration area aeration is used water-soluble
Solution oxygen concentration control be 0.5mg/L hereinafter, work as ammonia nitrogen removal frank be 80% or more, 60% or more nitrogen removal rate, into stabilization
Runtime completes the starting of reaction unit；
Every liter of sewage of simulation contains: NH4Cl 0.1g, NaHCO30.2g, pH7.0-7.5, surplus are water.
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| CN201610771705.2A CN106282020B (en) | 2016-08-30 | 2016-08-30 | A kind of method of the autotrophic denitrification micropopulation of orienting enriching different proportion |
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| CN201610771705.2A CN106282020B (en) | 2016-08-30 | 2016-08-30 | A kind of method of the autotrophic denitrification micropopulation of orienting enriching different proportion |
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| CN106282020B true CN106282020B (en) | 2019-07-12 |
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| CN107226600A (en) * | 2017-06-16 | 2017-10-03 | 广州泰通环保科技投资有限公司 | River bottom mud repair system |
| CN108611276A (en) * | 2018-04-16 | 2018-10-02 | 仲恺农业工程学院 | A method of recycling treatment being carried out to distillery waste water using microalgae |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020015606A (en) * | 2000-08-22 | 2002-02-28 | 김흥기 | complex wastewater treatment device using 3 level zeolite filter |
| CN203269716U (en) * | 2012-12-24 | 2013-11-06 | 深圳市深港产学研环保工程技术股份有限公司 | Artificial rapid infiltration system for industrial park sewage treatment |
-
2016
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020015606A (en) * | 2000-08-22 | 2002-02-28 | 김흥기 | complex wastewater treatment device using 3 level zeolite filter |
| CN203269716U (en) * | 2012-12-24 | 2013-11-06 | 深圳市深港产学研环保工程技术股份有限公司 | Artificial rapid infiltration system for industrial park sewage treatment |
Non-Patent Citations (4)
| Title |
|---|
| Preparation of ceramic filler from reusing sewage sludge and application in biological aerated filter for soy protein secondary wastewater treatment;Suqing Wu等;《Journal of Hazardous Materials》;20151231;第283卷;第608-616页 |
| SBＲ 系统外加磁场对微生物群落多样性和处理效果的影响;耿淑英等;《环境科学》;20171130;第38卷(第11期);第4715-4724页 |
| 分格复合填料曝气生物滤池脱氮除磷特性及微生物;郭小马等;《环境科学学报》;20150131;第35卷(第1期);第152-160页 |
| 浅谈SBR污水处理技术的进展;钟玉鸣;《广州环境科学》;20050930;第20卷(第3期);第20-27页 |
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Address after: Guangzhou City, Guangdong province 510070 martyrs Road No. 100 building No. 56 Patentee after: Guangdong Institute of Microbiology (Guangdong province microbiological analysis and testing center) Address before: Guangzhou City, Guangdong province 510070 martyrs Road No. 100 building No. 56 Patentee before: Guangdong Institute of Microbiology |