CN103484370B - A kind of method utilizing farm animal excrement waste water to cultivate spirulina - Google Patents
A kind of method utilizing farm animal excrement waste water to cultivate spirulina Download PDFInfo
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- CN103484370B CN103484370B CN201310387428.1A CN201310387428A CN103484370B CN 103484370 B CN103484370 B CN 103484370B CN 201310387428 A CN201310387428 A CN 201310387428A CN 103484370 B CN103484370 B CN 103484370B
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- spirulina
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention provides a kind of method utilizing farm animal excrement waste water to cultivate spirulina, belong to sewage disposal and algal culture field.The pre-treatment of the method comprises the steps: (1) farm animal excrement waste water: filter, get filtrate and carry out sterilizing, obtains nutrient solution; (2) domestication of spirulina: algae kind is inoculated in fresh water or seawater and carries out domestication cultivation? (3) cultivation of spirulina: the spirulina of logarithmic phase in step (2) is inoculated in the nutrient solution of step (1) and cultivates; (4) the gathering of spirulina: step (3) is cultivated gained spirulina and filters, cleaning, regathers spirulina, then carry out drying to spirulina; (5) filtrate is reclaimed.The invention solves farm animal excrement contaminate environment, problem that SPIRULINA CULTIVATION cost is high.The method can realize protection of the environment, reduces SPIRULINA CULTIVATION cost and can produce triple effects of necessary for human nutritive substance.
Description
Technical field
The present invention relates to sewage disposal and algal culture field, particularly a kind of method utilizing farm animal excrement waste water to cultivate spirulina.
Background technology
Along with expanding economy, livestock and poultry farms in scale gets more and more, so the amount of the farm animal excrement focused on is very huge, if treatment process is improper, is easy to cause contaminate environment.The treatment process of the farm animal excrement of traditional livestock and poultry farm is: (1) directly discharges; (2) be thrown in pond and breed fish; (3) solid-liquid separation, makees compost solid drying, and liquid directly discharges; (4) utilize fermentation to produce biogas, biogas does fuel, and the liquid after fermentation directly discharges.Traditional method also can turn waste into wealth to a certain extent, refuse is fully utilized, but be finally all that the waste liquid containing a large amount of phosphoric acid salt, ammonium salt is directly discharged, cause the change of rivers, pond chemical oxygen demand (COD), and other is addicted to the algal bloom of phosphoric acid salt, ammonium salt, still rivers are polluted, change natural ecological environment.The method solving this pollution problem is decomposed farm animal excrement with algae, utilizes the phosphoric acid salt in waste water, ammonium salt to synthesize the nutritive substance of people's needs.
Spirulina is one of algae that the known nutritive ingredient of the current mankind is abundant, the most balanced, it contains rich in protein, VITAMIN, beta carotene, gamma linolenic acid, also contain the trace element of Chlorophyll A and multiple needed by human, these effective constituents have under certain condition and reduce cholesterol, improve human immunologic function, promote prostate gland synthesis, press down cancer anti-cancer and accelerate the multiple medicine and health care function such as wound healing, thus, the demand of spirulina is larger.Current cultivation spirulina mostly uses natural lake or fresh water or sea farming, still need to add the nutritive substance needed for growth of spirulina platensis, or process nutrient solution, to reach the condition of applicable growth of spirulina platensis, this will cause the toxigenic capacity of spirulina high.
Summary of the invention
In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of method utilizing farm animal excrement waste water to cultivate spirulina.The method utilizes farm animal excrement waste water as the raw material of SPIRULINA CULTIVATION, spirulina can utilize phosphate in waste water, ammonium salt to synthesize nutritive substance, can realize contaminated solution problem, reduces SPIRULINA CULTIVATION cost and can produce triple effects of necessary for human nutritive substance.
For solving the problem, the technical solution adopted in the present invention is as follows:
Utilize farm animal excrement waste water to cultivate a method for spirulina, comprise the steps:
(1) pre-treatment of farm animal excrement waste water: filter, get filtrate and carry out sterilizing, obtains nutrient solution;
(2) domestication of spirulina: algae kind is inoculated in fresh water or seawater and carries out domestication cultivation;
(3) cultivation of spirulina: the spirulina of logarithmic phase in step (2) is inoculated in the nutrient solution of step (1) and cultivates;
(4) the gathering of spirulina: step (3) is cultivated gained spirulina and filters, cleaning, regathers spirulina, then carry out drying to spirulina;
(5) filtrate is reclaimed.
Preferably, the filter residue after filtering in step (1) is carried out drying, makes organic fertilizer.
Preferably, in step (1), before sterilization filtrate PH is adjusted to 8.6-9.5.
Preferably, the filtrate of step (1) also through the anaerobically fermenting of 2-3 days, carried out deodorizing before sterilizing, collected the biogas that fermentation produces simultaneously.
Preferably, in step (2), when adopting fresh water acclimatization, fresh water is the water meeting GB5749-85 sanitary standard for drinking water; When adopting seawater acclimation, the practical salinity of described seawater is less than 35, and acclimation temperature is 30 DEG C ~ 37 DEG C, pH8.0 ~ 10.0.
Preferably, step (3) spirulina is placed in U-shaped runway to cultivate, and the pH in culturing process remains on 7.2-10.5, illumination maintains 8000-20000LX, temperature 20-40 DEG C, and do not stop to stir, and incubation time is 10-14 days.
Preferably, in culturing process, pH remains on 8.6-9.5, illumination maintains 8000-12000LX, temperature remains on 25-35 DEG C.
Preferably, the wavelength of light is 430-500nm or 640-660nm.
Preferably, the aeration rate in culturing process is 150m3/hr-250m3/hr.
Preferably, step (4) is with fresh water cleaning and gathering screw algae after filtering, then at 80 DEG C dry 2 hours.
Compared to existing technology, beneficial effect of the present invention is:
One, farm animal excrement is filtered gained waste residue as fertilizer; filtrate is as the nutrient solution of spirulina; the biogas produced in SPIRULINA CULTIVATION process is as fuel; the phosphide of the drainage after gathering screw algae and the content of nitride are less than 5% of the phosphide of original waste water and the content of nitride; reach the requirement of GB/T18920-2002 " urban reclaimed water water quality standard "; can carry out second stage employ, farm animal excrement is fully used, and ecotope is protected.
Two, utilize waste water to cultivate spirulina, save cultivation water and reagent in SPIRULINA CULTIVATION process; Use U-shaped runway to cultivate, decrease cultivation land used, thus reduce the cost of SPIRULINA CULTIVATION.
Three, SPIRULINA CULTIVATION efficiency spirulina mass that is high, that obtain is high.Method of the present invention cultivates spirulina, its long speed ratio classical culture protocols fast 35%, and that the content of nutritive substance and traditional method are cultivated is consistent, and in addition, the lead tolerance of final gained spirulina is less than 0.3mg/kg, far below national standard.
Embodiment
A kind of method utilizing farm animal excrement waste water to cultivate spirulina of the present invention comprises:
(1) pre-treatment of farm animal excrement waste water:
The object of this step is farm animal excrement waste water to process, and becomes the nutrient solution being applicable to growth of spirulina platensis, and waste water pre-treatment is mainly through filtration, pH value adjustment and sterilizing.First be that farm animal excrement and wash-down water thereof are filtered, remove thick slag, using after thick slag drying as organic fertilizer; Again the pH value of filtrate is adjusted to 8.6-9.5, preferably adjust with HCl and NaOH, this scope is the PH of the most applicable growth of spirulina platensis; Then filtrate is carried out sterilizing, preferably carry out sterilizing with ozone; Gained liquid is the nutrient solution of spirulina.As one preferred embodiment, before sterilization steps, filtrate can be carried out anaerobically fermenting 2-3 days, deodorizing object can be reached, collect the biogas produced.
(2) domestication of spirulina:
Spirulina algae kind received in fresh water or seawater and carry out domestication cultivation, fresh water used meets GB5749-85 sanitary standard for drinking water; The practical salinity of seawater is less than 35, and in domestication step, temperature controls at 35 DEG C, and can within the scope of 30 DEG C ~ 37 DEG C, and pH maintains 8.0 ~ 10.0, and this temperature and pH value are optimum growth temp and the pH value of spirulina.
(3) cultivation of spirulina:
The spirulina of logarithmic phase in step (2) is inoculated in the nutrient solution of step (1) gained according to the ratio of 1:3-1:6, and cultivate in U-shaped runway, in the process, PH remains on 7.2-10.5, illumination maintains 8000-20000LX, temperature controls at 35 DEG C, and can within the scope of 20-40 DEG C, and stirring per hour 1 time, incubation time is 10-14 days.The condition optimization cultivated is: pH value 8.6 ~ 9.5, illumination 8000-12000LX, temperature 25 DEG C-35 DEG C.The wavelength of light is preferably 430-500nm or 640-660nm; Aeration rate is 150m
3/ hr-250m
3/ hr.Collect the biogas that this process produces.Wherein, aeration rate refers to by the amount of the methods such as inflation or mechanical agitation to transfer air in nutrient solution.
(4) the gathering of spirulina:
Filtered by spirulina liquor, preferred screen cloth filters, and then repeatedly cleans the spirulina on screen cloth, regathers the spirulina on screen cloth, finally at 80 DEG C dry 2 hours.
(5) filtrate is reclaimed: screen filtration gained filtrate can be reclaimed, because spirulina has employed phosphoric acid salt in waste liquid and ammonium salt, filtrate reaches GB/T18920-2002 " urban reclaimed water water quality standard ", and this water can carry out second stage employ, such as, the diluent of intrasystem stoste can be done, pigsty rinse with, water flowers and plants and flushing road, or be directly used in flushing pig house, can also as the drinking-water of livestock and poultry, if discharged, also meet emission standard.
Wherein, the order of step of the present invention (1) and step (2) is not certain, can successively carry out, and can carry out simultaneously yet.
Below, the present invention is described further to cultivate spirulina for the waste water of 1000 pigs:
Embodiment one
(1) pre-treatment of pig excrements waste water:
First be that farm animal excrement and wash-down water thereof are filtered, remove thick slag, using after thick slag drying as organic fertilizer; With HCl and NaOH, the pH value of filtrate is adjusted to 8.6 again, then passes into ozone and sterilizing is carried out to filtrate; Gained liquid is the nutrient solution of spirulina.
(2) domestication of spirulina:
Spirulina algae kind is received in seawater and carries out domestication cultivation, seawater used; In domestication step, temperature controls at 30 DEG C, and pH maintains 8.0.
(3) cultivation of spirulina:
The spirulina of logarithmic phase in step (2) is inoculated in the nutrient solution of step (1) gained according to the ratio of 1:3, and cultivate in U-shaped runway, in the process, PH remains on 7.2, illumination maintains 20000LX, temperature keeps 20 DEG C, and ceaselessly stir, incubation time is 12 days.The wavelength of light is 430nm, and aeration rate is 150m
3/ hr.Collect the biogas that this process produces.
(4) the gathering of spirulina:
Filtered by spirulina liquor, preferred screen cloth filters, and then repeatedly cleans the spirulina on screen cloth, regathers the spirulina on screen cloth, finally at 80 DEG C dry 2 hours.
(5) filtrate is reclaimed.
The filtrate that the present embodiment finally reclaims: PH is 7.2, and total phosphorus concentration is 27mg/L, and ammonium salt concentration is 39mg/L, and the output of the spirulina of acquisition is 13 tons/year; Lead tolerance 0.2mg/kg.
Embodiment two
(1) pre-treatment of pig excrements waste water:
First be that farm animal excrement and wash-down water thereof are filtered, remove thick slag, using after thick slag drying as organic fertilizer; Again filtrate is carried out anaerobically fermenting 2 days, reach deodorizing object, collect the biogas produced; With HCl and NaOH, the pH value of filtrate is adjusted to 9.0 again, then passes into ozone and sterilizing is carried out to filtrate; Gained liquid is the nutrient solution of spirulina.
(2) domestication of spirulina:
Spirulina algae kind received in seawater and carry out domestication cultivation, the practical salinity of seawater used is less than 35; In domestication step, temperature controls at 35 DEG C, and pH maintains 9.0.
(3) cultivation of spirulina:
The spirulina of logarithmic phase in step (2) is inoculated in the nutrient solution of step (1) gained according to the ratio of 1:6, and cultivate in U-shaped runway, in the process, PH remains on 9.0, illumination maintains 15000LX, temperature keeps 30 DEG C, and ceaselessly stir, incubation time is 10 days.The wavelength of light is 500nm, and aeration rate is 200m
3/ hr.Collect the biogas that this process produces.
(4) the gathering of spirulina:
Filtered by spirulina liquor, preferred screen cloth filters, and then repeatedly cleans the spirulina on screen cloth, regathers the spirulina on screen cloth, finally at 80 DEG C dry 2 hours.
(5) filtrate is reclaimed.
The filtrate that the present embodiment finally reclaims: PH is 7, and total phosphorus concentration is 20mg/L, and ammonium salt concentration is 48mg/L, and the output of the spirulina of acquisition is 12 tons/year; Lead tolerance 0.1mg/kg.
Embodiment three
(1) pre-treatment of pig excrements waste water:
First be that farm animal excrement and wash-down water thereof are filtered, remove thick slag, using after thick slag drying as organic fertilizer; Again filtrate is carried out anaerobically fermenting 3 days, reach deodorizing object, collect the biogas produced; With HCl and NaOH, the pH value of filtrate is adjusted to 9.5 again, then passes into ozone and sterilizing is carried out to filtrate; Gained liquid is the nutrient solution of spirulina.
(2) domestication of spirulina:
Spirulina algae kind received in seawater and carry out domestication cultivation, the practical salinity of seawater used is less than 35; In domestication step, temperature controls at 37 DEG C, and pH maintains 10.0.
(3) cultivation of spirulina:
The spirulina of logarithmic phase in step (2) is inoculated in the nutrient solution of step (1) gained according to the ratio of 1:4, and cultivate in U-shaped runway, in the process, PH remains on 10.5, illumination maintains 8000LX, temperature keeps 40 DEG C, and ceaselessly stir, incubation time is 14 days.The wavelength of light is 640nm, and aeration rate is 250m
3/ hr.Collect the biogas that this process produces.
(4) the gathering of spirulina:
Filtered by spirulina liquor, preferred screen cloth filters, and then repeatedly cleans the spirulina on screen cloth, regathers the spirulina on screen cloth, finally at 80 DEG C dry 2 hours.
(5) filtrate is reclaimed.
The filtrate that the present embodiment finally reclaims: PH is 7.5, and total phosphorus concentration is 25mg/L, and ammonium salt concentration is 52mg/L, and the output of the spirulina of acquisition is 14 tons/year; Lead tolerance 0.2mg/kg.
Embodiment four
(1) pre-treatment of pig excrements waste water:
First be that farm animal excrement and wash-down water thereof are filtered, remove thick slag, using after thick slag drying as organic fertilizer; With HCL and NaOH, the pH value of filtrate is adjusted to 9.2 again, then passes into ozone and sterilizing is carried out to filtrate; Gained liquid is the nutrient solution of spirulina.
(2) domestication of spirulina:
Spirulina algae kind received in fresh water and carry out domestication cultivation, fresh water used meets GB5749-85 sanitary standard for drinking water; In domestication step, temperature controls at 32 DEG C, and pH maintains 9.5.
(3) cultivation of spirulina:
The spirulina of logarithmic phase in step (2) is inoculated in the nutrient solution of step (1) gained according to the ratio of 1:5, and cultivate in U-shaped runway, in the process, PH remains on 9.5, illumination maintains 17000LX, temperature keeps 35 DEG C, and do not stop to stir, incubation time is 13 days.The wavelength of light is 660nm, and aeration rate is 230/hr.Collect the biogas that this process produces.
(4) the gathering of spirulina:
Filtered by spirulina liquor, preferred screen cloth filters, and then repeatedly cleans the spirulina on screen cloth, regathers the spirulina on screen cloth, finally at 80 DEG C dry 2 hours.
(5) filtrate is reclaimed.
The filtrate that the present embodiment finally reclaims: PH is 7.2, and total phosphorus concentration is 18mg/L, and ammonium salt concentration is 43mg/L, and the output of the spirulina of acquisition is 13 tons/year; Lead tolerance 0.1mg/kg.
Above-mentioned embodiment is only the preferred embodiment of the present invention; can not limit the scope of protection of the invention with this, change and the replacement of any unsubstantiality that those skilled in the art does on basis of the present invention all belong to the present invention's scope required for protection.
Claims (1)
1. utilize farm animal excrement waste water to cultivate a method for spirulina, it is characterized in that, comprise the steps:
(1) pre-treatment of pig excrements waste water:
First be that farm animal excrement and wash-down water thereof are filtered, remove thick slag, using after thick slag drying as organic fertilizer; Again filtrate is carried out anaerobically fermenting 3 days, reach deodorizing object, collect the biogas produced; With HCl and NaOH, the pH value of filtrate is adjusted to 9.5 again, then passes into ozone and sterilizing is carried out to filtrate; Gained liquid is the nutrient solution of spirulina;
(2) domestication of spirulina:
Spirulina algae kind received in seawater and carry out domestication cultivation, the practical salinity of seawater used is less than 35; In domestication step, temperature controls at 37 DEG C, and pH value maintains 10.0;
(3) cultivation of spirulina:
The spirulina of logarithmic phase in step (2) is inoculated in the nutrient solution of step (1) gained according to the ratio of 1:4, and cultivate in U-shaped runway, in the process, pH value remains on 10.5, illumination maintains 8000LX, temperature keeps 40 DEG C, and ceaselessly stir, incubation time is 14 days; The wavelength of light is 640nm, and aeration rate is 250m
3/ hr; Collect the biogas that this process produces;
(4) the gathering of spirulina:
Spirulina liquor screen cloth is filtered, then the spirulina on screen cloth is repeatedly cleaned, regather the spirulina on screen cloth, finally at 80 DEG C dry 2 hours;
(5) filtrate is reclaimed.
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| CN103988768B (en) * | 2014-05-30 | 2016-01-27 | 内蒙古科技大学 | A kind of method utilizing spirulina breeding waste water vegetable aquaculture |
| CN104988105A (en) * | 2015-08-14 | 2015-10-21 | 黄观铭 | Spiral seaweed cultivation method based on diversified illumination |
| CN105018348A (en) * | 2015-08-14 | 2015-11-04 | 黄观铭 | Spirulina culturing method based on staged feeding |
| CN104988068A (en) * | 2015-08-14 | 2015-10-21 | 黄观铭 | Method for culturing spirulina |
| CN104988106A (en) * | 2015-08-14 | 2015-10-21 | 黄观铭 | Method for culturing spirulina |
| CN106746177A (en) * | 2016-11-16 | 2017-05-31 | 洮南市龙兴牧业有限公司 | A kind of excrement dirt method for innocent treatment |
| CN107198038B (en) * | 2017-05-24 | 2021-01-15 | 河西学院 | Chlorella powder and spirulina powder composition and feed prepared from composition and used for breeding healthy pigs |
| CN107384832B (en) * | 2017-08-28 | 2020-06-09 | 中国科学院水生生物研究所 | Method for culturing nitrogen-fixing blue algae in large quantity at low cost |
| CN108059566A (en) * | 2018-01-18 | 2018-05-22 | 迪斯科科技集团（宜昌）有限公司 | A kind of poly spirulina peptide biostimulant |
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